WO2005097982A3 - Pufa-pks gene aus ulkenia - Google Patents

Pufa-pks gene aus ulkenia Download PDF

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Publication number
WO2005097982A3
WO2005097982A3 PCT/EP2005/003701 EP2005003701W WO2005097982A3 WO 2005097982 A3 WO2005097982 A3 WO 2005097982A3 EP 2005003701 W EP2005003701 W EP 2005003701W WO 2005097982 A3 WO2005097982 A3 WO 2005097982A3
Authority
WO
WIPO (PCT)
Prior art keywords
ulkenia
pufa
pks
pks genes
sequences
Prior art date
Application number
PCT/EP2005/003701
Other languages
English (en)
French (fr)
Other versions
WO2005097982A2 (de
Inventor
Markus Luy
Matthias Ruesing
Thomas Kiy
Original Assignee
Nutrinova Gmbh
Markus Luy
Matthias Ruesing
Thomas Kiy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nutrinova Gmbh, Markus Luy, Matthias Ruesing, Thomas Kiy filed Critical Nutrinova Gmbh
Priority to KR1020067023437A priority Critical patent/KR101484097B1/ko
Priority to BRPI0509747-9A priority patent/BRPI0509747A/pt
Priority to KR1020137020015A priority patent/KR20130114225A/ko
Priority to US11/547,921 priority patent/US7939305B2/en
Priority to JP2007506732A priority patent/JP2007532104A/ja
Priority to CA002563427A priority patent/CA2563427A1/en
Priority to EP05751638A priority patent/EP1733029A2/de
Priority to AU2005231964A priority patent/AU2005231964B2/en
Publication of WO2005097982A2 publication Critical patent/WO2005097982A2/de
Priority to IL178613A priority patent/IL178613A0/en
Publication of WO2005097982A3 publication Critical patent/WO2005097982A3/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

Die Erfindung beschreibt Gene, welche für Polyketidsynthasen (PKS) spezifische Sequenzen kodieren. Die daraus synthetisierte PKS ist charakterisiert durch ihre enzymatische Fähigkeit PUFAs (polyunsaturated fatty acids) zu produzieren. Die Erfindung umfasst weiterhin die Identifizierung der entsprechenden DNA-Sequenzen sowie den Einsatz der Nukleotidsequenzen für die Herstellung rekombinanter bzw. transgener Organismen.
PCT/EP2005/003701 2004-04-08 2005-04-08 Pufa-pks gene aus ulkenia WO2005097982A2 (de)

Priority Applications (9)

Application Number Priority Date Filing Date Title
KR1020067023437A KR101484097B1 (ko) 2004-04-08 2005-04-08 울케니아의 ｐｕｆａ―ｐｋｓ 유전자
BRPI0509747-9A BRPI0509747A (pt) 2004-04-08 2005-04-08 gens pufa-pks de ulkenia
KR1020137020015A KR20130114225A (ko) 2004-04-08 2005-04-08 울케니아의 ｐｕｆａ―ｐｋｓ 유전자
US11/547,921 US7939305B2 (en) 2004-04-08 2005-04-08 PUFA-PKS genes from Ulkenia
JP2007506732A JP2007532104A (ja) 2004-04-08 2005-04-08 ウルケニア（Ｕｌｋｅｎｉａ）由来のＰＵＦＡ−ＰＫＳ遺伝子
CA002563427A CA2563427A1 (en) 2004-04-08 2005-04-08 Pufa-pks genes from ulkenia
EP05751638A EP1733029A2 (de) 2004-04-08 2005-04-08 Pufa-pks gene aus ulkenia
AU2005231964A AU2005231964B2 (en) 2004-04-08 2005-04-08 PUFA-PKS genes from ulkenia
IL178613A IL178613A0 (en) 2004-04-08 2006-10-15 Pufa-pks genes from ulkenia

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004017370.2 2004-04-08
DE102004017370A DE102004017370A1 (de) 2004-04-08 2004-04-08 PUFA-PKS Gene aus Ulkenia

Publications (2)

Publication Number Publication Date
WO2005097982A2 WO2005097982A2 (de) 2005-10-20
WO2005097982A3 true WO2005097982A3 (de) 2007-04-05

Family

ID=35062272

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2005/003701 WO2005097982A2 (de) 2004-04-08 2005-04-08 Pufa-pks gene aus ulkenia

Country Status (11)

Country Link
US (1) US7939305B2 (de)
EP (1) EP1733029A2 (de)
JP (2) JP2007532104A (de)
KR (2) KR20130114225A (de)
CN (2) CN101087882A (de)
AU (1) AU2005231964B2 (de)
BR (1) BRPI0509747A (de)
CA (1) CA2563427A1 (de)
DE (1) DE102004017370A1 (de)
IL (1) IL178613A0 (de)
WO (1) WO2005097982A2 (de)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8853432B2 (en) 2004-04-22 2014-10-07 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cell
CN108753810B (zh) * 2018-05-22 2021-06-18 昆明理工大学 一种转录调节蛋白基因orf2的用途

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US5340742A (en) 1988-09-07 1994-08-23 Omegatech Inc. Process for growing thraustochytrium and schizochytrium using non-chloride salts to produce a microfloral biomass having omega-3-highly unsaturated fatty acids
US8003772B2 (en) 1999-01-14 2011-08-23 Martek Biosciences Corporation Chimeric PUFA polyketide synthase systems and uses thereof
KR20100116233A (ko) * 2000-01-28 2010-10-29 마텍 바이오싸이언스스 코포레이션 발효기 내에서 진핵 미생물의 고밀도 배양에 의한 고도불포화 지방산을 함유하는 지질의 증진된 생산 방법
CA3056163C (en) 2004-04-22 2021-04-06 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
WO2007106903A2 (en) * 2006-03-15 2007-09-20 Martek Biosciences Corporation Polyunsaturated fatty acid production in heterologous organisms using pufa polyketide synthase systems
CN101578363A (zh) 2006-08-29 2009-11-11 联邦科学技术研究组织 脂肪酸的合成
ES2644883T3 (es) 2008-11-18 2017-11-30 Commonwealth Scientific And Industrial Research Organisation Enzimas y métodos para producir ácidos grasos omega-3
ES2623610T3 (es) 2009-03-19 2017-07-11 Dsm Ip Assets B.V. Moléculas de ácido nucleico de ácido graso poliinsaturado sintasas, y polipéptidos, composiciones y métodos de preparación y usos de los mismos
US9062315B2 (en) 2010-10-01 2015-06-23 Kyushu University, National University Corporation Method for transformation of stramenopile
SG11201408362SA (en) 2012-06-15 2015-01-29 Commw Scient Ind Res Org Production of long chain polyunsaturated fatty acids in plant cells
CA2933909A1 (en) 2013-12-18 2015-06-25 Grains Research And Development Corporation Lipid comprising long chain polyunsaturated fatty acids
WO2015116671A2 (en) 2014-01-28 2015-08-06 Dsm Ip Assets B.V. Factors for the production and accumulation of polyunsaturated fatty acids (pufas) derived from pufa synthases
CA2953008C (en) 2014-06-27 2024-03-19 Nuseed Pty Ltd Lipid comprising docosapentaenoic acid
US9932599B2 (en) * 2015-03-02 2018-04-03 Synthetic Genomics, Inc. Regulatory elements from labyrinthulomycetes microorganisms
WO2017161005A1 (en) 2016-03-16 2017-09-21 Synthetic Genomics, Inc. Production of proteins in labyrinthulomycetes
KR102442450B1 (ko) 2016-05-12 2022-09-14 디에스엠 아이피 어셋츠 비.브이. 미세조류에서 오메가-3 다중불포화 지방산 생산을 증가시키는 방법
JOP20170154B1 (ar) 2016-08-01 2023-03-28 Omeros Corp تركيبات وطرق لتثبيط masp-3 لعلاج أمراض واضطرابات مختلفة
US10633454B2 (en) 2016-11-01 2020-04-28 Conagen Inc. Expression of modified glycoproteins and glycopeptides
WO2018219171A1 (zh) * 2017-05-31 2018-12-06 厦门汇盛生物有限公司 一株生产dha和epa的细菌、该细菌基因组中的6个基因片段及它们的应用
WO2020032258A1 (ja) * 2018-08-10 2020-02-13 協和発酵バイオ株式会社 多価不飽和脂肪酸を生産する微生物及び多価不飽和脂肪酸の製造法
WO2020032261A1 (ja) * 2018-08-10 2020-02-13 協和発酵バイオ株式会社 エイコサペンタエン酸を生産する微生物及びエイコサペンタエン酸の製造法
CN110577921B (zh) * 2019-05-28 2021-04-02 浙江工业大学 产两性霉素b的重组结节链霉菌及其应用
CN114107074B (zh) * 2021-11-18 2024-04-09 厦门大学 一种过表达3-酮酰基合酶基因的裂殖壶菌基因工程菌株的构建方法及其应用

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8853432B2 (en) 2004-04-22 2014-10-07 Commonwealth Scientific And Industrial Research Organisation Synthesis of long-chain polyunsaturated fatty acids by recombinant cell
CN108753810B (zh) * 2018-05-22 2021-06-18 昆明理工大学 一种转录调节蛋白基因orf2的用途

Also Published As

Publication number Publication date
CN103981156A (zh) 2014-08-13
WO2005097982A2 (de) 2005-10-20
JP2007532104A (ja) 2007-11-15
US7939305B2 (en) 2011-05-10
KR20130114225A (ko) 2013-10-16
CN101087882A (zh) 2007-12-12
KR101484097B1 (ko) 2015-01-23
DE102004017370A1 (de) 2005-10-27
BRPI0509747A (pt) 2007-09-25
AU2005231964B2 (en) 2012-03-08
IL178613A0 (en) 2007-02-11
KR20070056002A (ko) 2007-05-31
US20090093033A1 (en) 2009-04-09
EP1733029A2 (de) 2006-12-20
AU2005231964A1 (en) 2005-10-20
JP2012205595A (ja) 2012-10-25
CA2563427A1 (en) 2005-10-20

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