WO2007136736A3 - Methods for nucleic acid sorting and synthesis - Google Patents

Methods for nucleic acid sorting and synthesis Download PDF

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Publication number
WO2007136736A3
WO2007136736A3 PCT/US2007/011886 US2007011886W WO2007136736A3 WO 2007136736 A3 WO2007136736 A3 WO 2007136736A3 US 2007011886 W US2007011886 W US 2007011886W WO 2007136736 A3 WO2007136736 A3 WO 2007136736A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
nucleic acid
synthesis
sorting
acid sorting
Prior art date
Application number
PCT/US2007/011886
Other languages
French (fr)
Other versions
WO2007136736A2 (en
Inventor
George Church
Francisco Cerrina
Brian M Baynes
Edmund R Pitcher
Original Assignee
Codon Devices Inc
George Church
Francisco Cerrina
Brian M Baynes
Edmund R Pitcher
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Codon Devices Inc, George Church, Francisco Cerrina, Brian M Baynes, Edmund R Pitcher filed Critical Codon Devices Inc
Publication of WO2007136736A2 publication Critical patent/WO2007136736A2/en
Publication of WO2007136736A3 publication Critical patent/WO2007136736A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Abstract

Methods of sorting oligonucleotide sequences are provided. The methods include determining the sequences of oligonucleotides and selectively isolating one or more that are determined to have the desired sequence. Methods of nucleic acid preparation are also disclosed.
PCT/US2007/011886 2006-05-19 2007-05-18 Methods for nucleic acid sorting and synthesis WO2007136736A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US80219706P 2006-05-19 2006-05-19
US60/802,197 2006-05-19

Publications (2)

Publication Number Publication Date
WO2007136736A2 WO2007136736A2 (en) 2007-11-29
WO2007136736A3 true WO2007136736A3 (en) 2008-01-24

Family

ID=38578429

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2007/011886 WO2007136736A2 (en) 2006-05-19 2007-05-18 Methods for nucleic acid sorting and synthesis

Country Status (1)

Country Link
WO (1) WO2007136736A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US11845054B2 (en) 2010-11-12 2023-12-19 Gen9, Inc. Methods and devices for nucleic acids synthesis

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8053191B2 (en) 2006-08-31 2011-11-08 Westend Asset Clearinghouse Company, Llc Iterative nucleic acid assembly using activation of vector-encoded traits
US8568979B2 (en) 2006-10-10 2013-10-29 Illumina, Inc. Compositions and methods for representational selection of nucleic acids from complex mixtures using hybridization
WO2010025310A2 (en) 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Methods and devices for high fidelity polynucleotide synthesis
EP3257942B1 (en) 2008-12-18 2020-07-22 ITI Scotland Limited Method for assembly of polynucleic acid sequences
US9347092B2 (en) 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
JP5457222B2 (en) 2009-02-25 2014-04-02 エフ．ホフマン−ラ ロシュ アーゲー Miniaturized high-throughput nucleic acid analysis
PL2414369T3 (en) 2009-04-02 2016-04-29 Fundacion Centro Nac De Investigaciones Oncologicas Carlos Iii Imidazo[2,1-b][1,3,4]thiadiazole derivatives
US10207240B2 (en) 2009-11-03 2019-02-19 Gen9, Inc. Methods and microfluidic devices for the manipulation of droplets in high fidelity polynucleotide assembly
GB2481425A (en) 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
US10457935B2 (en) 2010-11-12 2019-10-29 Gen9, Inc. Protein arrays and methods of using and making the same
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
JP2014526899A (en) 2011-08-26 2014-10-09 ジェン９・インコーポレイテッド Compositions and methods for high fidelity assembly of nucleic acids
WO2013123125A1 (en) * 2012-02-17 2013-08-22 President And Fellows Of Harvard College Assembly of nucleic acid sequences in emulsions
US9150853B2 (en) 2012-03-21 2015-10-06 Gen9, Inc. Methods for screening proteins using DNA encoded chemical libraries as templates for enzyme catalysis
EP4001427A1 (en) * 2012-04-24 2022-05-25 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
AU2013280661A1 (en) 2012-06-25 2015-01-22 Gen9, Inc. Methods for nucleic acid assembly and high throughput sequencing

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WO2005078130A1 (en) * 2004-02-10 2005-08-25 Solexa Limited Arrayed polynucleotides
WO2006026614A2 (en) * 2004-08-27 2006-03-09 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations

Patent Citations (4)

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US20050079510A1 (en) * 2003-01-29 2005-04-14 Jan Berka Bead emulsion nucleic acid amplification
US20050130173A1 (en) * 2003-01-29 2005-06-16 Leamon John H. Methods of amplifying and sequencing nucleic acids
WO2005078130A1 (en) * 2004-02-10 2005-08-25 Solexa Limited Arrayed polynucleotides
WO2006026614A2 (en) * 2004-08-27 2006-03-09 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations

Non-Patent Citations (7)

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Title
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MARGULIES M. ET AL.: "Genome sequencing in microfabricated high-density picolitre reactors", NATURE, NATURE PUBLISHING GROUP, LONDON, GB, vol. 437, no. 7057, 15 September 2005 (2005-09-15), pages 376 - 380, XP002398505, ISSN: 0028-0836 *
MITRA R.D. ET AL.: "Fluorescent in situ sequencing on polymerase colonies", ANALYTICAL BIOCHEMISTRY, ACADEMIC PRESS, SAN DIEGO, CA, US, vol. 320, no. 1, 1 September 2003 (2003-09-01), pages 55 - 65, XP004441395, ISSN: 0003-2697 *
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9216414B2 (en) 2009-11-25 2015-12-22 Gen9, Inc. Microfluidic devices and methods for gene synthesis
US9217144B2 (en) 2010-01-07 2015-12-22 Gen9, Inc. Assembly of high fidelity polynucleotides
US11845054B2 (en) 2010-11-12 2023-12-19 Gen9, Inc. Methods and devices for nucleic acids synthesis

Also Published As

Publication number Publication date
WO2007136736A2 (en) 2007-11-29

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