WO2005062025A1 - Verfahren zur spektroskopischen analyse einer biologischen oder chemischen substanz - Google Patents
Verfahren zur spektroskopischen analyse einer biologischen oder chemischen substanz Download PDFInfo
- Publication number
- WO2005062025A1 WO2005062025A1 PCT/EP2004/014397 EP2004014397W WO2005062025A1 WO 2005062025 A1 WO2005062025 A1 WO 2005062025A1 EP 2004014397 W EP2004014397 W EP 2004014397W WO 2005062025 A1 WO2005062025 A1 WO 2005062025A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- sample
- electromagnetic radiation
- ionized
- substance
- spectrum
- Prior art date
Links
- 239000000126 substance Substances 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 19
- 238000004611 spectroscopical analysis Methods 0.000 title claims description 9
- 230000005670 electromagnetic radiation Effects 0.000 claims abstract description 28
- 238000001228 spectrum Methods 0.000 claims abstract description 9
- 230000003595 spectral effect Effects 0.000 claims abstract description 8
- 238000001514 detection method Methods 0.000 claims description 6
- 239000002245 particle Substances 0.000 claims description 5
- 238000010884 ion-beam technique Methods 0.000 claims description 4
- 238000000132 electrospray ionisation Methods 0.000 claims 1
- 230000005855 radiation Effects 0.000 description 10
- 238000004458 analytical method Methods 0.000 description 7
- 238000011835 investigation Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000003993 interaction Effects 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000027455 binding Effects 0.000 description 1
- 238000009739 binding Methods 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000009878 intermolecular interaction Effects 0.000 description 1
- 230000008863 intramolecular interaction Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 230000012846 protein folding Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3577—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing liquids, e.g. polluted water
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3581—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using far infrared light; using Terahertz radiation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3563—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing solids; Preparation of samples therefor
Definitions
- the invention relates to a method for the spectroscopic analysis of a preferably biological or chemical substance, in which a sample of the substance is irradiated with electromagnetic radiation, the spectrum of the electromagnetic radiation influenced by the sample is registered, and the spectral distribution is used to infer the sample properties becomes.
- the object of the invention is to further develop methods of the type described at the outset such that the examination of biological or chemical substances by means of electromagnetic radiation, the frequencies of which are in the infrared range and above, also leads to meaningful results in their native state.
- the spectrum of the electromagnetic radiation influenced by the sample is registered and it is concluded from the spectral distribution that the sample properties are derived the sample is at least partially ionized, electromagnetic radiation with a frequency in the range of infrared, gigahertz or terahertz frequency is used, and the influence of ionized sample parts on the spectrum of the electromagnetic radiation is registered and evaluated.
- spectroscopy at frequencies in the ranges mentioned is possible for the first time for the investigation of substances, in particular biological molecules in their native state.
- the method can also be used for spectroscopic examinations in which molecules remain in their native state on the one hand, but on the other hand background disturbances are also to be minimized, in particular because in this way the volume of the interfering water content in the sample is reduced. This is the case, for example, when analyzing samples in physico-chemistry, in medical diagnostics, drug discovery, genomics and proteomics.
- the background reduction is essentially achieved by generating small, electrically charged droplets of the sample with the ionization of the sample or of sample parts and registering and evaluating their influence on the spectrum of the electromagnetic radiation.
- droplets of the order of 100 to 1 ⁇ m are generated, so that on the one hand the disturbing absorption of the solvent, such as water, is minimized as far as possible, but the chemical environment of the sample is not significantly disturbed.
- the application extends not only to terahertz spectroscopy, but also to spectroscopy with electromagnetic radiation in the infrared and in the gigahertz frequency range.
- the ionization of the sample or the formation of small electrically charged droplets can be realized in a technologically advantageous manner by an electrospray apparatus, as described, for example, by M. Wilm et al. (1996), "Femtomole sequencing of proteins from polyacrylamide gels by nanoelectrospray ass spectrometry", Nature, Vol. 379 (6564), pp. 466-469.
- Embodiments of the method according to the invention are also advantageous, in which systems for beam processing are used in order to influence the direction and / or the distribution of the ion beam in the analysis space, if necessary.
- electromagnetic lenses such as quadrupole lenses
- So-called ion cages which are known per se from the prior art, are advantageously used for ion storage. They allow a longer integration time of the sample particles, which means that the amount of sample substance required for a significant analysis can be kept low.
- all conventional radiation sources which emit radiation in the range of the infrared frequencies and in the gigahertz range, but in particular terahertz radiation sources, can serve as sources of the electromagnetic radiation.
- Radar radiation sources may also be suitable for carrying out the method according to the invention.
- a sample 1 to be analyzed a device 2 for ionizing sample 1 or at least a part thereof, a system 3 for beam processing, a source 4 for electromagnetic radiation and a detection device 5 for receiving samples 1 electromagnetic radiation is suitable and communicates with an evaluation device which is not shown in the drawing.
- sample 1 can come from a wide variety of fields in biology or chemistry.
- sample 1 is a biological substance in which the receptor-ligand interaction is to be investigated.
- sample droplets subsequently enter the system 3 for beam processing, which can be used to influence or change the direction and the distribution of the sample droplets within an analysis space 6 as required.
- the electromagnetic radiation is received with the aid of the detection device 5.
- the received signals are fed to the evaluation device and evaluated there in a manner known per se with regard to the spectral distribution of the electromagnetic radiation. From the comparison of the spectral distribution of the uninfluenced by the sample radiation with the spectral distribution of d he influenced sample radiation conclusions are drawn on the properties of Sample 1 and obtained information about the corresponding molecules in their In ⁇ formation content over the previous possibilities prior go beyond technology.
Landscapes
- Physics & Mathematics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Toxicology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
Description
Claims
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE2003161903 DE10361903A1 (de) | 2003-12-22 | 2003-12-22 | Verfahren zur spektroskopischen Analyse einer biologischen oder chemischen Substanz |
DE10361903.8 | 2003-12-22 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2005062025A1 true WO2005062025A1 (de) | 2005-07-07 |
Family
ID=34706737
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/EP2004/014397 WO2005062025A1 (de) | 2003-12-22 | 2004-12-17 | Verfahren zur spektroskopischen analyse einer biologischen oder chemischen substanz |
Country Status (2)
Country | Link |
---|---|
DE (1) | DE10361903A1 (de) |
WO (1) | WO2005062025A1 (de) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7355420B2 (en) | 2001-08-21 | 2008-04-08 | Cascade Microtech, Inc. | Membrane probing system |
US7420381B2 (en) | 2004-09-13 | 2008-09-02 | Cascade Microtech, Inc. | Double sided probing structures |
US7492172B2 (en) | 2003-05-23 | 2009-02-17 | Cascade Microtech, Inc. | Chuck for holding a device under test |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5247842A (en) * | 1991-09-30 | 1993-09-28 | Tsi Incorporated | Electrospray apparatus for producing uniform submicrometer droplets |
US20020113144A1 (en) * | 1999-09-06 | 2002-08-22 | Hitachi, Ltd. | Analytical apparatus using nebulizer |
US20020158196A1 (en) * | 2001-03-29 | 2002-10-31 | Berggren William Travis | Piezoelectric charged droplet source |
Family Cites Families (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4036167A (en) * | 1976-01-30 | 1977-07-19 | Inficon Leybold-Heraeus Inc. | Apparatus for monitoring vacuum deposition processes |
DD127021B1 (de) * | 1976-04-29 | 1979-12-27 | Joachim Mohr | Vorrichtung fuer spektrochemische untersuchungen, insbesondere fuer die laser-mikrospektralanalyse |
DD127779A1 (de) * | 1976-05-27 | 1977-10-12 | Winfried Quillfeldt | Anordnung fuer die laser-spektralanalyse |
DE4006790A1 (de) * | 1990-03-03 | 1991-09-12 | Kernforschungsz Karlsruhe | Verfahren zur reduktion der spektralen breite von anregungsspektrallinien |
DE10006361A1 (de) * | 1999-05-25 | 2000-11-30 | Deutsche Telekom Ag | Miniaturisierte Terahertz-Strahlungsquelle |
DE10002970B4 (de) * | 2000-01-25 | 2004-09-16 | Gkss-Forschungszentrum Geesthacht Gmbh | Vorrichtung zur Analyse von in tröpfchenförmigen Flüssigkeitsproben enthaltenen Elementen |
DE10002969A1 (de) * | 2000-01-25 | 2001-08-02 | Geesthacht Gkss Forschung | Vorrichtung zur Analyse von in Flüssigkeitsproben enthaltenen Elementen |
DE10054476A1 (de) * | 2000-07-10 | 2002-01-31 | Bolivar Peter Haring | Verfahren zum Nachweis von Polynucleotidsequenzen |
DE10125849A1 (de) * | 2001-05-25 | 2002-11-28 | Carbotec Ges Fuer Instr Analyt | Elektrosprayvorrichtung |
-
2003
- 2003-12-22 DE DE2003161903 patent/DE10361903A1/de not_active Withdrawn
-
2004
- 2004-12-17 WO PCT/EP2004/014397 patent/WO2005062025A1/de active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5247842A (en) * | 1991-09-30 | 1993-09-28 | Tsi Incorporated | Electrospray apparatus for producing uniform submicrometer droplets |
US20020113144A1 (en) * | 1999-09-06 | 2002-08-22 | Hitachi, Ltd. | Analytical apparatus using nebulizer |
US20020158196A1 (en) * | 2001-03-29 | 2002-10-31 | Berggren William Travis | Piezoelectric charged droplet source |
Non-Patent Citations (1)
Title |
---|
NAGEL M ET AL: "Integrated THz technology for label-free genetic diagnostics", APPLIED PHYSICS LETTERS, AMERICAN INSTITUTE OF PHYSICS. NEW YORK, US, vol. 80, no. 1, 7 January 2002 (2002-01-07), pages 154 - 156, XP012030208, ISSN: 0003-6951 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7355420B2 (en) | 2001-08-21 | 2008-04-08 | Cascade Microtech, Inc. | Membrane probing system |
US7492175B2 (en) | 2001-08-21 | 2009-02-17 | Cascade Microtech, Inc. | Membrane probing system |
US7492172B2 (en) | 2003-05-23 | 2009-02-17 | Cascade Microtech, Inc. | Chuck for holding a device under test |
US7876115B2 (en) | 2003-05-23 | 2011-01-25 | Cascade Microtech, Inc. | Chuck for holding a device under test |
US7420381B2 (en) | 2004-09-13 | 2008-09-02 | Cascade Microtech, Inc. | Double sided probing structures |
US8013623B2 (en) | 2004-09-13 | 2011-09-06 | Cascade Microtech, Inc. | Double sided probing structures |
Also Published As
Publication number | Publication date |
---|---|
DE10361903A1 (de) | 2005-07-28 |
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