WO2001005938A1 - Procede d'obtention de champignons de ganoderma lucidum - Google Patents

Procede d'obtention de champignons de ganoderma lucidum Download PDF

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Publication number
WO2001005938A1
WO2001005938A1 PCT/CN2000/000201 CN0000201W WO0105938A1 WO 2001005938 A1 WO2001005938 A1 WO 2001005938A1 CN 0000201 W CN0000201 W CN 0000201W WO 0105938 A1 WO0105938 A1 WO 0105938A1
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WO
WIPO (PCT)
Prior art keywords
weight
ppm
ganoderma lucidum
selenium
ganoderma
Prior art date
Application number
PCT/CN2000/000201
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English (en)
Chinese (zh)
Inventor
Quancai Li
Original Assignee
Quancai Li
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN 99108733 external-priority patent/CN1277060A/zh
Application filed by Quancai Li filed Critical Quancai Li
Publication of WO2001005938A1 publication Critical patent/WO2001005938A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution

Definitions

  • the present invention relates to a method for producing ganoderma, and in particular, the present invention relates to a method for producing ganoderma mycelium containing selenium and other various elements. current technology
  • Ganoderma G an0 dermaLucidum The taste is mild and the temperature is warm, which can nourish the deficiency of the cold, and replenish the solid.
  • Selenium is one of the essential trace elements in the human body. Selenium is found in many functional proteases in the body. Compared with inorganic selenium, organic selenium is more conducive to human body absorption and has good safety. Selenium compounds can scavenge lipid free radicals and hydroxyl radicals. Selenium can affect the expression and regulation of cancer cell genes, and selenium intervention has been used to obtain good anti-cancer effects in cancer-prone areas. China has a low-selenium zone covering 15 provinces from northeast to southwest. The average daily selenium for adults in China is 26.42ug, and the normal daily demand for adults is 50-200ug.
  • iron, zinc, copper, manganese, selenium, chromium, fluorine, and iodine are essential elements of the human body.
  • the human body's requirements for these elements are: 10-20 mg of iron, 15 mg of zinc, 2-3 mg of copper, 3 ⁇ 4 50-200 g, 2.5-5 mg of manganese, 100ug of cobalt, 50-120ug of chromium, and 2 of fluorine 1-3mg, iodine 1.5mg.
  • These elements each have their special functions for the synthesis of certain adenine and hormones in the human body, energy conversion, reproduction, growth, development, brain thinking and memory, information transmission, and transmission of the genetic code.
  • Elements are the only basic nutrients that cannot be synthesized by the body itself. When the elements coexist in the human body, there is not only a quantitative relationship, but more importantly, a proportional relationship and a complex interaction, which are specifically manifested in synergy and antagonism, or both.
  • the existence of trace elements in Chinese medicine is mainly to form complexes with organic matter.
  • the amino acids, peptides, proteins, and nucleic acids contained in the mycelium of Ganoderma lucidum can be used as excellent complexes of chromium compounds.
  • Ganoderma lucidum mycelium synthesizes complexes with selenium and a series of trace elements, which not only enriches traditional Chinese medicine, but also provides a new approach for the study of trace elements in traditional Chinese medicine.
  • the purpose of the present invention is to adopt an advanced liquid deep fermentation process, use a special liquid culture solution adapted to the process, and add selenium and a series of trace elements to prepare a multi-linguine ganoderma selenium mycelium dry product and a water agent, and make it It can supplement biological selenium for selenium-deficient people, and has curative effects on liver, cardiovascular diseases, tumors, and AIDS prevention.
  • the invention provides a method for producing ganoderma mycelium, including:
  • red ganoderma mycelium was cultured in a medium of 50-200ppm sodium selenite;
  • the medium for cultivating red ganoderma mycelium of the present invention preferably further contains ferrous sulfate 200-1000 ppm, zinc sulfate 500-3000 ppm, copper sulfate 50-200 ppm, and manganese sulfate 100-500 ppm.
  • ferrous sulfate 200-1000 ppm preferably further contains ferrous sulfate 200-1000 ppm, zinc sulfate 500-3000 ppm, copper sulfate 50-200 ppm, and manganese sulfate 100-500 ppm.
  • the biological selenium dry product selenium is> 1000 ppm
  • the aqueous agent selenium is> 50 ppm. Its toxicity is extremely low, and its maximum tolerance to mice is 1080 times higher than the maximum safe intake of human diet. Examples
  • the primary seed tank is 50 liters, the fixed volume is 35 liters, and the inoculation volume is 1500-2000 ml (5% -7%).
  • 500 liters of secondary seed tank 350 liters of fixed volume, 35 liters (10%) of primary seed liquid.
  • sterilize the seed tank (135'C-140 ° C, 2.5-2.8 kg / cm 2 ) for 1 hour, fill the fermentation broth and then sterilize the fermentation broth: preheat the interlayer to 90 'C, 10 After a few minutes, the steam was raised to 115 V, 1.2 kg / cm 2 , and the temperature was kept constant for 8 minutes. Then the temperature was lowered and the temperature was reduced to 28 ° C.
  • Inoculation must use a dedicated inoculation tube and inoculation bottle. Fermentation temperature is 25'C—28 ° C, PH5.0-5.5, ventilation volume 1: 0.5—1.0, stirring speed is 180 rpm. Quality control sampling during the whole fermentation: at 0, check for miscellaneous bacteria, 0D value, initial sugar: 12, 24, 36, 48 hours, check for 0D, residual sugar, PH value. 48 hours normal value: PH5, 0, 0D net length of 0.5, one unit of sugar consumption.
  • the 5 ton fermentation tank has a fixed capacity of 3500 liters and an inoculation volume of 350 liters.
  • the high-temperature boiled seed oil is added as a defoamer at 2% of the fermentation broth.
  • the fermentation temperature is 25'C-26'C.
  • the initial ventilation is 1: 0.5, and it can be increased to 1 in the middle. : 1.
  • Stirring rate is 180 rpm. Measure sugar, nitrogen, and pH every 12 hours after 72 hours of culture. Measure sugar, nitrogen, pH, and sterility every 12 hours after 72 hours of incubation.
  • the standard of irrigation is : PH5, residual sugar 2.5, ammonia nitrogen ⁇ 30 ⁇ g / ml.
  • the qualified fermentation liquid in the fermentor was centrifuged, rinsed with sterile water, filtered with 100 mesh, and freeze-dried to obtain dried Ganoderma lucidum selenium. Add twice the sterile water to the washed mycelium, and heat it to 50 ° C. After 35-50 minutes, the mycelium will dissolve automatically. After aseptic filling, the mycelium solution will be obtained.
  • Duolian Ganoderma selenium dry product contains selenium> 1000ppm.
  • the liquid preparation contains selenium> 50 ⁇ ! 11, and the contents of iron, zinc, copper, manganese, iodine, chromium, fluorine, and boron all exceed the content of conventional ganoderma mycelium fermentation products.

Abstract

L'invention concerne un procédé d'obtention de champignons de Ganoderma lucidum consistant à cultiver les germes Ganoderma lucidum CGMCC 0475 dans un milieu contenant entre 1,5 et 2,5 % en poids de glucose, entre 2 et 3 % en poids de poudre de maïs, entre 0,5 et 1 % en poids de peptone, entre 1, 5 et 2,5 % en poids d'extrait de boeuf, entre 0,1 et 0,2 % en poids de Kh2PO4, entre 30 et 50 ppm de Na2SeO3 ayant un pH compris entre 5,0 et 5,5 ; à inoculer la culture précitée dans le milieu contenant entre 1 et 2 % en poids de glucose, entre 3 et 4 % en poids de poudre de maïs, entre 0,5 et 1 % en poids de peptone, entre 2 et 3 % en poids d'extrait de boeuf, entre 30 et 50 ppm de Na2SeO3 ayant un pH compris entre 5,0 et 5,5 de manière à obtenir les champignons de Ganoderma lucidum; c) à purifier et à préparer ces champignons. Lesdits champignons contiennent > 1000 ppm de sélénium (poids sec) ou > 50 ppm de sélénium (poids humide).
PCT/CN2000/000201 1999-07-15 2000-07-14 Procede d'obtention de champignons de ganoderma lucidum WO2001005938A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN 99108733 CN1277060A (zh) 1999-06-11 1999-07-15 多元灵芝硒的生产方法
CN99108733.X 1999-07-15

Publications (1)

Publication Number Publication Date
WO2001005938A1 true WO2001005938A1 (fr) 2001-01-25

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Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT500435A1 (de) * 2004-05-18 2005-12-15 G N V Gesundheits Und Naturpro Nahrungsergänzungsmittel
SG129276A1 (en) * 2003-04-10 2007-02-26 Auric Pacific Nutritech Pte Lt Large-scale production method for cordyceps and ganoderma
CN104620853A (zh) * 2015-02-04 2015-05-20 深圳福山生物科技有限公司 一种富硒灵芝孢子粉或子实体的培育方法
CN105009930A (zh) * 2014-04-25 2015-11-04 江苏中祥高科技实业有限公司 一种富硒灵芝菌丝体生产方法
CN105176832A (zh) * 2015-07-23 2015-12-23 广西仙草堂制药有限责任公司 一种富硒灵芝菌种的制作方法
US20170280629A1 (en) * 2016-04-01 2017-10-05 Ami Cosmetic Co., Ltd. Methods of Producing Ganoderma Lucidum Mycelia Having Enhanced Ganodermanondiol Content
CN110257261A (zh) * 2019-07-04 2019-09-20 硒利技术研发有限公司 一种富硒牛樟芝菌丝体的生产方法
CN112353719A (zh) * 2020-11-02 2021-02-12 郭光波 一种灵芝抗衰老面霜及其制备方法
CN113767812A (zh) * 2021-09-23 2021-12-10 山西农业大学山西功能食品研究院 一种灵芝菌株及其红肉灵芝子实体的培养方法
CN114214208A (zh) * 2021-12-14 2022-03-22 江南大学 一株高耐硒灵芝突变体菌株及其应用
CN114250153A (zh) * 2021-12-15 2022-03-29 江南大学 高耐硒灵芝Ganoderma lucidum JNUSE-200及其富硒发酵策略
CN115053752A (zh) * 2022-06-30 2022-09-16 安徽金寨仙芝灵生物科技有限公司 一种新型灵芝菌种的培育方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1170758A (zh) * 1996-07-17 1998-01-21 谭新国 富含多种微量元素的灵芝菌丝体及其生产方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1170758A (zh) * 1996-07-17 1998-01-21 谭新国 富含多种微量元素的灵芝菌丝体及其生产方法

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG129276A1 (en) * 2003-04-10 2007-02-26 Auric Pacific Nutritech Pte Lt Large-scale production method for cordyceps and ganoderma
AT500435A1 (de) * 2004-05-18 2005-12-15 G N V Gesundheits Und Naturpro Nahrungsergänzungsmittel
AT500435B1 (de) * 2004-05-18 2006-10-15 G N V Gesundheits Und Naturpro Aphrodisiakum
CN105009930A (zh) * 2014-04-25 2015-11-04 江苏中祥高科技实业有限公司 一种富硒灵芝菌丝体生产方法
CN105009930B (zh) * 2014-04-25 2018-03-09 江苏中祥高科技实业有限公司 一种富硒灵芝菌丝体生产方法
CN104620853A (zh) * 2015-02-04 2015-05-20 深圳福山生物科技有限公司 一种富硒灵芝孢子粉或子实体的培育方法
CN105176832A (zh) * 2015-07-23 2015-12-23 广西仙草堂制药有限责任公司 一种富硒灵芝菌种的制作方法
US20170280629A1 (en) * 2016-04-01 2017-10-05 Ami Cosmetic Co., Ltd. Methods of Producing Ganoderma Lucidum Mycelia Having Enhanced Ganodermanondiol Content
CN110257261A (zh) * 2019-07-04 2019-09-20 硒利技术研发有限公司 一种富硒牛樟芝菌丝体的生产方法
CN112353719A (zh) * 2020-11-02 2021-02-12 郭光波 一种灵芝抗衰老面霜及其制备方法
CN113767812A (zh) * 2021-09-23 2021-12-10 山西农业大学山西功能食品研究院 一种灵芝菌株及其红肉灵芝子实体的培养方法
CN113767812B (zh) * 2021-09-23 2023-04-25 山西农业大学山西功能食品研究院 一种灵芝菌株及其红肉灵芝子实体的培养方法
CN114214208A (zh) * 2021-12-14 2022-03-22 江南大学 一株高耐硒灵芝突变体菌株及其应用
CN114250153A (zh) * 2021-12-15 2022-03-29 江南大学 高耐硒灵芝Ganoderma lucidum JNUSE-200及其富硒发酵策略
CN114250153B (zh) * 2021-12-15 2023-07-25 江南大学 高耐硒灵芝Ganoderma lucidum JNUSE-200及其富硒发酵策略
CN115053752A (zh) * 2022-06-30 2022-09-16 安徽金寨仙芝灵生物科技有限公司 一种新型灵芝菌种的培育方法

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