WO1999052356A1 - Creation de tissus tridimensionnels - Google Patents

Creation de tissus tridimensionnels Download PDF

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Publication number
WO1999052356A1
WO1999052356A1 PCT/US1999/007816 US9907816W WO9952356A1 WO 1999052356 A1 WO1999052356 A1 WO 1999052356A1 US 9907816 W US9907816 W US 9907816W WO 9952356 A1 WO9952356 A1 WO 9952356A1
Authority
WO
WIPO (PCT)
Prior art keywords
cells
vascularized
providing
constructs
dimensional tissue
Prior art date
Application number
PCT/US1999/007816
Other languages
English (en)
Inventor
Craig R. Halberstadt
Walter D. Holder, Jr.
Original Assignee
Charlotte-Mecklenberg Hospital Authority
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Charlotte-Mecklenberg Hospital Authority filed Critical Charlotte-Mecklenberg Hospital Authority
Priority to JP2000542979A priority Critical patent/JP2002511284A/ja
Priority to CA002330104A priority patent/CA2330104A1/fr
Priority to AU35520/99A priority patent/AU3552099A/en
Priority to EP99917384A priority patent/EP1069822A1/fr
Publication of WO1999052356A1 publication Critical patent/WO1999052356A1/fr

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/74—Alginate

Definitions

  • Figure 11 is a bar graph comparing width of alginate-RGD bead implant at different times.
  • the macroporous lattice structure of the porous construct is formed during the process of polymerization of one or more polymers using conventional methods for making macroporous structures.
  • the macropores in the construct are formed by dissolution of a polymer or removal of one material after polymerization.
  • the construct is formed from a porous matrix of polymers in which the pores are formed by dissolution of one of the polymers.
  • the second construct need not be macroporous.
  • the second construct contains signals which stimulate the formation of a vascularized bed.
  • An example of such signal is the tri- peptide arginine-glycine-aspartate (RGD). This tri-peptide can be optimally linked to the second construct.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Transplantation (AREA)
  • Microbiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Dermatology (AREA)
  • Vascular Medicine (AREA)
  • Epidemiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Botany (AREA)
  • Materials For Medical Uses (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne un procédé d'apport d'un tissu tridimensionnel vascularisé dans un sujet vivant. Le procédé consiste à) à créer à partir d'une matière biocompatible capable de support l'adhésion, la croissance et la migration cellulaires, une construction poreuse contenant des cellules à transplanter et b) à administrer la construction dans une zone d'intérêt dans le sujet vivant pour former un tissu tridimensionnel vascularisé. La construction préférentielle présente une dimension dont la distance de la surface la plus externe au centre de la construction mesure entre environ 50 νm et environ 500 νm. La construction préférentielle présente aussi une structure poreuse interconnectée dont la granulométrie des pores est de l'ordre d'environ 10 νm ne dépassant pas 300νm. Les cellules contenues dans la construction préférentielle ne dépassent pas une distance de 250 νm à partir de la surface externe de la construction.
PCT/US1999/007816 1998-04-09 1999-04-09 Creation de tissus tridimensionnels WO1999052356A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2000542979A JP2002511284A (ja) 1998-04-09 1999-04-09 三次元組織の創造
CA002330104A CA2330104A1 (fr) 1998-04-09 1999-04-09 Creation de tissus tridimensionnels
AU35520/99A AU3552099A (en) 1998-04-09 1999-04-09 Creation of three-dimensional tissues
EP99917384A EP1069822A1 (fr) 1998-04-09 1999-04-09 Creation de tissus tridimensionnels

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5861998A 1998-04-09 1998-04-09
US09/058,619 1998-04-09

Publications (1)

Publication Number Publication Date
WO1999052356A1 true WO1999052356A1 (fr) 1999-10-21

Family

ID=22017933

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/007816 WO1999052356A1 (fr) 1998-04-09 1999-04-09 Creation de tissus tridimensionnels

Country Status (5)

Country Link
EP (1) EP1069822A1 (fr)
JP (1) JP2002511284A (fr)
AU (1) AU3552099A (fr)
CA (1) CA2330104A1 (fr)
WO (1) WO1999052356A1 (fr)

Cited By (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000066036A3 (fr) * 1999-04-30 2001-02-08 Massachusetts Gen Hospital Fabrication de tissus vascularises a l'aide de moules bidimensionnels microfabriques
EP1230939A1 (fr) * 2001-02-13 2002-08-14 Haverich, Axel, Prof. Dr. Matrice tissulaire bioartificielle vascularisée et tissu bioartificiel vascularisé
WO2002078439A2 (fr) * 2001-03-30 2002-10-10 The Arizona Board Of Regents On Behalf Of The University Of Arizona Constructions prevascularisees d'implantation destinees a fournir une perfusion sanguine
WO2003018752A2 (fr) * 2001-08-23 2003-03-06 The Wistar Institute Of Anatomy And Biology Culture organotypique intestinale et son utilisation
JP2003516364A (ja) * 1999-12-08 2003-05-13 ウラディミロビチ ズィービン，ドミトリー 哺乳動物の生体組織内でカプセルを形成するためのポリアクリルアミドゲルの使用方法、細胞培養方法並びに癌及び真性糖尿病の治療法
WO2003082366A1 (fr) * 2002-03-28 2003-10-09 Japan As Represented By President Of National Cardiovascular Center Materiau support d'ingenierie tissulaire, vaisseau artificiel, element de manchette et revetement destine a des implants
WO2004112860A1 (fr) * 2003-06-18 2004-12-29 Hokkaido Technology Licensing Office Co., Ltd. Materiau pour l'adhesion et la proliferation cellulaire
WO2005061019A2 (fr) * 2003-12-10 2005-07-07 Cellular Bioengineering, Inc. (Cbi) Procedes et composition de reconstruction de caracteristiques de tissus mous
DE19952847B4 (de) * 1999-10-01 2006-03-23 Minuth, Will, Prof. Dr. Vorrichtung zum Kultivieren und/oder Differenzieren und/oder Halten von Zellen und/oder Geweben
US7265199B2 (en) 2000-04-11 2007-09-04 Celonova Biosciences Germany Gmbh Poly-tri-fluoro-ethoxypolyphosphazene coverings and films
US20080025956A1 (en) * 2004-02-09 2008-01-31 Indiana University Research And Technology Corporation Blood vessel formation from endothelial colony forming cells
WO2008060822A2 (fr) * 2006-10-20 2008-05-22 Keracure, Inc. Dispositifs à cellules mise en culture sur des supports flexibles
WO2009048314A1 (fr) * 2007-10-08 2009-04-16 Sureshan Sivananthan Matrice pouvant être mise à l'échelle pour la culture in vivo d'os et de cartilage
US7670797B2 (en) 2003-01-16 2010-03-02 The General Hospital Corporation Method of determining toxicity with three dimensional structures
US7776021B2 (en) 2000-04-28 2010-08-17 The Charles Stark Draper Laboratory Micromachined bilayer unit for filtration of small molecules
US7922764B2 (en) 2006-10-10 2011-04-12 Celonova Bioscience, Inc. Bioprosthetic heart valve with polyphosphazene
US8007821B2 (en) 2001-01-11 2011-08-30 Celonova Biosciences Germany Gmbh Substrates containing polyphosphazene as matrices and substrates containing polyphosphazene with microstructured surface
US8080418B2 (en) 2007-03-09 2011-12-20 Corning Incorporated Method of making a three dimensional cell culture matrix
US8361781B2 (en) 2006-01-24 2013-01-29 Brown University Cell aggregation and encapsulation device and method
US8500822B2 (en) 2004-06-01 2013-08-06 Dai Nippon Printing Co., Ltd. Artificial tissue construct and method for producing the same
US8501476B2 (en) 2009-10-07 2013-08-06 Brown University Assays and methods for fusing cell aggregates to form proto-tissues
US9080146B2 (en) 2001-01-11 2015-07-14 Celonova Biosciences, Inc. Substrates containing polyphosphazene as matrices and substrates containing polyphosphazene with a micro-structured surface
US9107850B2 (en) 2004-10-25 2015-08-18 Celonova Biosciences, Inc. Color-coded and sized loadable polymeric particles for therapeutic and/or diagnostic applications and methods of preparing and using the same
US9114162B2 (en) 2004-10-25 2015-08-25 Celonova Biosciences, Inc. Loadable polymeric particles for enhanced imaging in clinical applications and methods of preparing and using the same
US9243278B2 (en) 2011-09-22 2016-01-26 Brown University Mechanotransduction by the synergistic action of heterotypic cell interactions
US9468680B2 (en) 2011-09-22 2016-10-18 Brown University Differential effects of drugs on transport in a multi-layer 3D spheroid model
US10973770B2 (en) 2004-10-25 2021-04-13 Varian Medical Systems, Inc. Color-coded and sized loadable polymeric particles for therapeutic and/or diagnostic applications and methods of preparing and using the same
US11096997B2 (en) 2005-12-13 2021-08-24 President And Fellows Of Harvard College Scaffolds for cell transplantation
US11278604B2 (en) 2012-04-16 2022-03-22 President And Fellows Of Harvard College Mesoporous silica compositions comprising inflammatory cytokines comprising inflammatory cytokines for modulating immune responses
US11555177B2 (en) 2016-07-13 2023-01-17 President And Fellows Of Harvard College Antigen-presenting cell-mimetic scaffolds and methods for making and using the same
US11752238B2 (en) 2016-02-06 2023-09-12 President And Fellows Of Harvard College Recapitulating the hematopoietic niche to reconstitute immunity
US11786457B2 (en) 2015-01-30 2023-10-17 President And Fellows Of Harvard College Peritumoral and intratumoral materials for cancer therapy

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2004201641B2 (en) * 1999-04-30 2007-04-19 The Charles Stark Draper Laboratory, Inc. Fabrication of Vascularized Tissue Using Microfabricated Two-Dimensional Molds
WO2004072104A2 (fr) * 2003-02-11 2004-08-26 Northwestern University Procedes et matieres pour revetements de surface nanocristallins et liaison de nanofibres d'amphiphiles peptidiques sur ceux-ci
US20070233274A1 (en) * 2004-04-12 2007-10-04 Hideyuki Miyake Artificial Tissue and Process for Producing the Same
JP5024823B2 (ja) * 2007-05-30 2012-09-12 独立行政法人産業技術総合研究所 細胞運動性評価セルチップ
WO2010067904A1 (fr) * 2008-12-12 2010-06-17 国立大学法人東京大学 Procédé de co-culture cellulaire hiérarchique tridimensionnelle
KR102015738B1 (ko) 2015-09-11 2019-08-28 후지필름 가부시키가이샤 젤라틴 구조체 제조 방법, 및 젤라틴 구조체 제조 시스템

Citations (1)

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US5716404A (en) * 1994-12-16 1998-02-10 Massachusetts Institute Of Technology Breast tissue engineering

Patent Citations (1)

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Non-Patent Citations (2)

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Title
BARRERA D. A., ET AL.: "SYNTHESIS AND RGD PEPTIDE MODIFICATION OF A NEW BIODEGRADABLE COPOLYMER: POLY(LACTIC ACID-CO-LYSINE).", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, AMERICAN CHEMICAL SOCIETY, US, vol. 115., no. 23., 1 January 1993 (1993-01-01), US, pages 11010/11011., XP002921405, ISSN: 0002-7863, DOI: 10.1021/ja00076a077 *
GRANDE D. A., ET AL.: "EVALUATION OF MATRIX SCAFFOLDS FOR TISSUE ENGINEERING OF ARTICULAR CARTILAGE GRAFTS.", JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, WILEY, NEW YORK, NY, US, vol. 34., 1 January 1997 (1997-01-01), US, pages 211 - 220., XP002921406, ISSN: 0021-9304, DOI: 10.1002/(SICI)1097-4636(199702)34:2<211::AID-JBM10>3.0.CO;2-L *

Cited By (48)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000066036A3 (fr) * 1999-04-30 2001-02-08 Massachusetts Gen Hospital Fabrication de tissus vascularises a l'aide de moules bidimensionnels microfabriques
US6455311B1 (en) 1999-04-30 2002-09-24 The General Hospital Corporation Fabrication of vascularized tissue
DE19952847B4 (de) * 1999-10-01 2006-03-23 Minuth, Will, Prof. Dr. Vorrichtung zum Kultivieren und/oder Differenzieren und/oder Halten von Zellen und/oder Geweben
JP2003516364A (ja) * 1999-12-08 2003-05-13 ウラディミロビチ ズィービン，ドミトリー 哺乳動物の生体組織内でカプセルを形成するためのポリアクリルアミドゲルの使用方法、細胞培養方法並びに癌及び真性糖尿病の治療法
US7265199B2 (en) 2000-04-11 2007-09-04 Celonova Biosciences Germany Gmbh Poly-tri-fluoro-ethoxypolyphosphazene coverings and films
US7776021B2 (en) 2000-04-28 2010-08-17 The Charles Stark Draper Laboratory Micromachined bilayer unit for filtration of small molecules
US8007821B2 (en) 2001-01-11 2011-08-30 Celonova Biosciences Germany Gmbh Substrates containing polyphosphazene as matrices and substrates containing polyphosphazene with microstructured surface
US9080146B2 (en) 2001-01-11 2015-07-14 Celonova Biosciences, Inc. Substrates containing polyphosphazene as matrices and substrates containing polyphosphazene with a micro-structured surface
WO2002064179A3 (fr) * 2001-02-13 2002-11-21 Axel Haverich Matrice tissulaire bioartificielle a vascularisation primaire, tissu bioartificiel a vascularisation primaire, leur procede de realisation et leur utilisation
US7732125B2 (en) 2001-02-13 2010-06-08 Corlife Gbr Bioartificial primarily vascularized tissue matrix, and bioartificial primarily vascularized tissue, method for the production and use of the same
WO2002064179A2 (fr) * 2001-02-13 2002-08-22 Axel Haverich Matrice tissulaire bioartificielle a vascularisation primaire, tissu bioartificiel a vascularisation primaire, leur procede de realisation et leur utilisation
EP1230939A1 (fr) * 2001-02-13 2002-08-14 Haverich, Axel, Prof. Dr. Matrice tissulaire bioartificielle vascularisée et tissu bioartificiel vascularisé
WO2002078439A3 (fr) * 2001-03-30 2002-11-14 Univ Arizona Constructions prevascularisees d'implantation destinees a fournir une perfusion sanguine
WO2002078439A2 (fr) * 2001-03-30 2002-10-10 The Arizona Board Of Regents On Behalf Of The University Of Arizona Constructions prevascularisees d'implantation destinees a fournir une perfusion sanguine
US7217570B2 (en) 2001-08-23 2007-05-15 The Wistar Institute Of Anatomy And Biology Organotypic intestinal culture and methods of use thereof
WO2003018752A3 (fr) * 2001-08-23 2003-06-12 Wistar Inst Culture organotypique intestinale et son utilisation
WO2003018752A2 (fr) * 2001-08-23 2003-03-06 The Wistar Institute Of Anatomy And Biology Culture organotypique intestinale et son utilisation
WO2003082366A1 (fr) * 2002-03-28 2003-10-09 Japan As Represented By President Of National Cardiovascular Center Materiau support d'ingenierie tissulaire, vaisseau artificiel, element de manchette et revetement destine a des implants
US7670797B2 (en) 2003-01-16 2010-03-02 The General Hospital Corporation Method of determining toxicity with three dimensional structures
US8173361B2 (en) 2003-01-16 2012-05-08 The General Hospital Corporation Method of determining metabolism of a test agent
WO2004112860A1 (fr) * 2003-06-18 2004-12-29 Hokkaido Technology Licensing Office Co., Ltd. Materiau pour l'adhesion et la proliferation cellulaire
WO2005061019A2 (fr) * 2003-12-10 2005-07-07 Cellular Bioengineering, Inc. (Cbi) Procedes et composition de reconstruction de caracteristiques de tissus mous
US7753955B2 (en) 2003-12-10 2010-07-13 Cellular Bioengineering, Inc. Methods and composition for soft tissue feature reconstruction
AU2004305574B2 (en) * 2003-12-10 2010-08-26 Cellular Bioengineering, Inc. Methods and composition for soft tissue feature reconstruction
WO2005061019A3 (fr) * 2003-12-10 2005-09-22 Cellular Bioengineering Inc Cb Procedes et composition de reconstruction de caracteristiques de tissus mous
US20080025956A1 (en) * 2004-02-09 2008-01-31 Indiana University Research And Technology Corporation Blood vessel formation from endothelial colony forming cells
US9034648B2 (en) 2004-06-01 2015-05-19 Dai Nippon Printing Co., Ltd. Artificial tissue construct and method for producing the same
US8500822B2 (en) 2004-06-01 2013-08-06 Dai Nippon Printing Co., Ltd. Artificial tissue construct and method for producing the same
US10973770B2 (en) 2004-10-25 2021-04-13 Varian Medical Systems, Inc. Color-coded and sized loadable polymeric particles for therapeutic and/or diagnostic applications and methods of preparing and using the same
US9597419B2 (en) 2004-10-25 2017-03-21 Boston Scientific Limited Loadable polymeric particles for enhanced imaging in clinical applications and methods of preparing and using the same
US9114162B2 (en) 2004-10-25 2015-08-25 Celonova Biosciences, Inc. Loadable polymeric particles for enhanced imaging in clinical applications and methods of preparing and using the same
US9107850B2 (en) 2004-10-25 2015-08-18 Celonova Biosciences, Inc. Color-coded and sized loadable polymeric particles for therapeutic and/or diagnostic applications and methods of preparing and using the same
US11096997B2 (en) 2005-12-13 2021-08-24 President And Fellows Of Harvard College Scaffolds for cell transplantation
US9587213B2 (en) 2006-01-24 2017-03-07 Brown University Methods and devices for encapsulating cells
US8361781B2 (en) 2006-01-24 2013-01-29 Brown University Cell aggregation and encapsulation device and method
US7922764B2 (en) 2006-10-10 2011-04-12 Celonova Bioscience, Inc. Bioprosthetic heart valve with polyphosphazene
WO2008060822A2 (fr) * 2006-10-20 2008-05-22 Keracure, Inc. Dispositifs à cellules mise en culture sur des supports flexibles
WO2008060822A3 (fr) * 2006-10-20 2008-07-24 Keracure Inc Dispositifs à cellules mise en culture sur des supports flexibles
US8658851B2 (en) 2006-10-20 2014-02-25 Keracure, Inc. Devices with cells cultured on flexible supports
US8080418B2 (en) 2007-03-09 2011-12-20 Corning Incorporated Method of making a three dimensional cell culture matrix
WO2009048314A1 (fr) * 2007-10-08 2009-04-16 Sureshan Sivananthan Matrice pouvant être mise à l'échelle pour la culture in vivo d'os et de cartilage
US8501476B2 (en) 2009-10-07 2013-08-06 Brown University Assays and methods for fusing cell aggregates to form proto-tissues
US9468680B2 (en) 2011-09-22 2016-10-18 Brown University Differential effects of drugs on transport in a multi-layer 3D spheroid model
US9243278B2 (en) 2011-09-22 2016-01-26 Brown University Mechanotransduction by the synergistic action of heterotypic cell interactions
US11278604B2 (en) 2012-04-16 2022-03-22 President And Fellows Of Harvard College Mesoporous silica compositions comprising inflammatory cytokines comprising inflammatory cytokines for modulating immune responses
US11786457B2 (en) 2015-01-30 2023-10-17 President And Fellows Of Harvard College Peritumoral and intratumoral materials for cancer therapy
US11752238B2 (en) 2016-02-06 2023-09-12 President And Fellows Of Harvard College Recapitulating the hematopoietic niche to reconstitute immunity
US11555177B2 (en) 2016-07-13 2023-01-17 President And Fellows Of Harvard College Antigen-presenting cell-mimetic scaffolds and methods for making and using the same

Also Published As

Publication number Publication date
EP1069822A1 (fr) 2001-01-24
AU3552099A (en) 1999-11-01
JP2002511284A (ja) 2002-04-16
CA2330104A1 (fr) 1999-10-21

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