WO1999038874A2 - Electrophoretic nucleic acid purification method - Google Patents

Electrophoretic nucleic acid purification method Download PDF

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Publication number
WO1999038874A2
WO1999038874A2 PCT/US1999/001137 US9901137W WO9938874A2 WO 1999038874 A2 WO1999038874 A2 WO 1999038874A2 US 9901137 W US9901137 W US 9901137W WO 9938874 A2 WO9938874 A2 WO 9938874A2
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acid
electrophoresis
well
electrical field
matrix
Prior art date
Application number
PCT/US1999/001137
Other languages
English (en)
French (fr)
Other versions
WO1999038874A3 (en
Inventor
Gary Slater
J. William Efcavitch
Guy Drouin
Pascal Mayer
Jean Rousseau
Hong Yan Zhou
Claudia Chiesa
Robert Ruhfel
Roger O'neill
Original Assignee
The Perkin-Elmer Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by The Perkin-Elmer Corporation filed Critical The Perkin-Elmer Corporation
Priority to DE69928230T priority Critical patent/DE69928230T2/de
Priority to AT99902378T priority patent/ATE309260T1/de
Priority to EP99902378A priority patent/EP1056758B1/de
Priority to JP2000529341A priority patent/JP2002502020A/ja
Priority to AU22374/99A priority patent/AU734170B2/en
Priority to CA002318973A priority patent/CA2318973A1/en
Publication of WO1999038874A2 publication Critical patent/WO1999038874A2/en
Publication of WO1999038874A3 publication Critical patent/WO1999038874A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Electrochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Information Retrieval, Db Structures And Fs Structures Therefor (AREA)
  • Saccharide Compounds (AREA)
PCT/US1999/001137 1998-01-30 1999-01-19 Electrophoretic nucleic acid purification method WO1999038874A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
DE69928230T DE69928230T2 (de) 1998-01-30 1999-01-19 Elektrophoretisches nukleinsäure-aufreinigungsverfahren
AT99902378T ATE309260T1 (de) 1998-01-30 1999-01-19 Elektrophoretische reinigungsmethode von nukleinsäuren
EP99902378A EP1056758B1 (de) 1998-01-30 1999-01-19 Elektrophoretische reinigungsmethode von nukleinsäuren
JP2000529341A JP2002502020A (ja) 1998-01-30 1999-01-19 電気泳動による核酸精製法
AU22374/99A AU734170B2 (en) 1998-01-30 1999-01-19 Electrophoretic nucleic acid purification method
CA002318973A CA2318973A1 (en) 1998-01-30 1999-01-19 Electrophoretic nucleic acid purification method

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/016,531 1998-01-30
US09/016,531 US6146511A (en) 1998-01-30 1998-01-30 Electrophoretic nucleic acid purification method

Publications (2)

Publication Number Publication Date
WO1999038874A2 true WO1999038874A2 (en) 1999-08-05
WO1999038874A3 WO1999038874A3 (en) 1999-09-23

Family

ID=21777601

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1999/001137 WO1999038874A2 (en) 1998-01-30 1999-01-19 Electrophoretic nucleic acid purification method

Country Status (8)

Country Link
US (2) US6146511A (de)
EP (1) EP1056758B1 (de)
JP (1) JP2002502020A (de)
AT (1) ATE309260T1 (de)
AU (1) AU734170B2 (de)
CA (1) CA2318973A1 (de)
DE (1) DE69928230T2 (de)
WO (1) WO1999038874A2 (de)

Cited By (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004048398A1 (ja) * 2002-11-28 2004-06-10 Arkray Inc. 核酸の濃縮精製方法および装置
WO2005045023A1 (ja) 2003-11-10 2005-05-19 Arkray Inc. 核酸の濃縮精製方法および装置
CN1332972C (zh) * 2002-11-28 2007-08-22 爱科来株式会社 浓缩和纯化核酸的方法和装置
US8133371B2 (en) 2004-02-02 2012-03-13 The University Of British Columbia Scodaphoresis and methods and apparatus for moving and concentrating particles
US8182666B2 (en) 2005-02-07 2012-05-22 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
US8475641B2 (en) 2008-02-01 2013-07-02 The University Of British Columbia Methods and apparatus for particle introduction and recovery
US8518228B2 (en) 2011-05-20 2013-08-27 The University Of British Columbia Systems and methods for enhanced SCODA
US8529744B2 (en) 2004-02-02 2013-09-10 Boreal Genomics Corp. Enrichment of nucleic acid targets
US8877028B2 (en) 2009-04-21 2014-11-04 The University Of British Columbia System and methods for detection of particles
US9186685B2 (en) 2012-01-13 2015-11-17 The University Of British Columbia Multiple arm apparatus and methods for separation of particles
US9340835B2 (en) 2013-03-15 2016-05-17 Boreal Genomics Corp. Method for separating homoduplexed and heteroduplexed nucleic acids
US9512477B2 (en) 2012-05-04 2016-12-06 Boreal Genomics Inc. Biomarker anaylsis using scodaphoresis
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins
CN115228262A (zh) * 2022-08-22 2022-10-25 湖南国测生物科技有限公司 实验室密闭空间内核酸污染的立体净化方法

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6146511A (en) 1998-01-30 2000-11-14 The Perkin-Elmer Corporation Electrophoretic nucleic acid purification method
AU3931699A (en) * 1998-05-04 1999-11-23 Fraunhofer-Gesellschaft Zur Forderung Der Angewandten Forschung E.V. Method and device for isolating nucleic acids
AU2001274887A1 (en) * 2000-05-23 2001-12-03 Mj Geneworks, Inc. Electrophoretic stacking of dna and other samples prior to electrophoresis
JP2004527739A (ja) * 2001-03-08 2004-09-09 エスログ・バイオテクノロジー・リミテッド 電気泳動のための装置及び方法
US7503904B2 (en) * 2002-04-25 2009-03-17 Cardiac Pacemakers, Inc. Dual balloon telescoping guiding catheter
US6905585B2 (en) * 2002-09-11 2005-06-14 Temple University Of The Commonwealth System Of Higher Education Automated system for high-throughput electrophoretic separations
US7601491B2 (en) * 2003-02-06 2009-10-13 Becton, Dickinson And Company Pretreatment method for extraction of nucleic acid from biological samples and kits therefor
JP2005110503A (ja) * 2003-10-02 2005-04-28 Arkray Inc 核酸の精製方法及びデバイス
CN101317086B (zh) * 2005-10-04 2013-08-14 海德威技术公司 分析物的微观流体检测
JP4195039B2 (ja) * 2006-02-16 2008-12-10 善之 辻本 検体の前処理方法および試薬
KR100661482B1 (ko) * 2006-04-03 2006-12-27 전남대학교산학협력단 Dna 정제를 위한 수집홈을 갖는 전기영동장치
US20080047835A1 (en) * 2006-08-22 2008-02-28 Macconnell William P Genomic DNA Purifier
JP2010502962A (ja) * 2006-08-31 2010-01-28 ライフ テクノロジーズ コーポレーション 電気泳動ゲルから生体分子を単離し、かつ収集する方法、カセット、ゲル及び装置
EP2257796A2 (de) * 2008-03-03 2010-12-08 Oregon Health and Science University Verfahren zur reinigung biologischer makromoleküle
CA2772606A1 (en) 2009-09-01 2011-03-10 Oregon Health & Science University Reversible current gel electrophoresis device for separating biological macromolecules
JP5246128B2 (ja) * 2009-10-09 2013-07-24 凸版印刷株式会社 電気泳動用ゲルカセット製造方法及び電気泳動用ゲルの充填方法
US9952176B2 (en) * 2011-06-16 2018-04-24 British Columbia Cancer Agency Branch Automated size selection of nucleic acids
US11207679B2 (en) 2018-04-13 2021-12-28 Regents Of The University Of Minnesota DNA extraction device
JP6905961B2 (ja) * 2018-07-12 2021-07-21 株式会社日立ハイテク 電気泳動方法、電気泳動システム及び電気泳動ゲル
JP7471436B2 (ja) 2020-09-24 2024-04-19 株式会社日立ハイテク 電気泳動回収装置、及び核酸前処理装置
CN114277027A (zh) * 2021-12-23 2022-04-05 深圳大学 一种用于电泳分离物回收的用具及其使用方法

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Publication number Priority date Publication date Assignee Title
WO1986000989A1 (en) * 1984-08-01 1986-02-13 Schumacher Juergen Electrophoretic process for the separation of biopolymers
EP0545689A1 (de) * 1991-12-04 1993-06-09 University Of North Carolina At Chapel Hill Verbesserung der elektroforetischen Trennung von einzelnen DNA Strangen durch asymetrische Feldumkehrung
EP0644420A2 (de) * 1993-09-09 1995-03-22 The University Of North Carolina At Chapel Hill Verfahren und Vorrichtung für Gelelektrophorese
WO1995014921A1 (en) * 1993-11-23 1995-06-01 Perkin-Elmer Corporation Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
EP0773225A2 (de) * 1995-11-08 1997-05-14 The Perkin-Elmer Corporation Einschliessen von Matrizen zur Sequenzierung von Nukleinsäuren in Probenmischungen bei verflochtenen Polymernetzwerken

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JPS6113148A (ja) * 1984-06-29 1986-01-21 Hitachi Ltd 連続型核酸断片電気泳動装置
CA2012379C (en) * 1989-04-24 2000-01-25 Gary W. Slater Processes for the preparation and separation of macromolecules
US5284940A (en) * 1990-11-14 1994-02-08 Hri Research, Inc. Preparation for nucleic acid samples
US5217593A (en) * 1991-03-13 1993-06-08 Macconnell William P Nucleic acid purification system and method
US5139637A (en) * 1991-03-13 1992-08-18 Macconnell William P Plasmid purification system and method
US5209831A (en) * 1991-06-14 1993-05-11 Macconnell William P Bufferless electrophoresis system and method
DE69332852T2 (de) * 1992-09-11 2003-12-04 Hoffmann La Roche Nachweis von Nukleinsäuren im Blut
US5482836A (en) * 1993-01-14 1996-01-09 The Regents Of The University Of California DNA purification by triplex-affinity capture and affinity capture electrophoresis
US5650055A (en) * 1993-04-07 1997-07-22 Margolis; Joel Electrophoresis separation method and apparatus using barrier separation and polarity reversing
US5384022A (en) * 1993-09-08 1995-01-24 Cornell Research Foundation, Inc. Method and apparatus for electrophoretic DNA band isolation
US5538614A (en) * 1994-08-17 1996-07-23 Han; Dawn D. Macromolecule recovery cassette
US5587062A (en) * 1996-01-24 1996-12-24 Shimadzu Corporation Sample collecting apparatus by gel electrophoresis
US6120985A (en) * 1997-10-31 2000-09-19 Bbi Bioseq, Inc. Pressure-enhanced extraction and purification
US6146511A (en) 1998-01-30 2000-11-14 The Perkin-Elmer Corporation Electrophoretic nucleic acid purification method

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986000989A1 (en) * 1984-08-01 1986-02-13 Schumacher Juergen Electrophoretic process for the separation of biopolymers
EP0545689A1 (de) * 1991-12-04 1993-06-09 University Of North Carolina At Chapel Hill Verbesserung der elektroforetischen Trennung von einzelnen DNA Strangen durch asymetrische Feldumkehrung
EP0644420A2 (de) * 1993-09-09 1995-03-22 The University Of North Carolina At Chapel Hill Verfahren und Vorrichtung für Gelelektrophorese
WO1995014921A1 (en) * 1993-11-23 1995-06-01 Perkin-Elmer Corporation Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
WO1995014922A1 (en) * 1993-11-23 1995-06-01 Perkin-Elmer Corporation Entrapment of nucleic acid sequencing template in sample mixtures by entangled polymer networks
EP0773225A2 (de) * 1995-11-08 1997-05-14 The Perkin-Elmer Corporation Einschliessen von Matrizen zur Sequenzierung von Nukleinsäuren in Probenmischungen bei verflochtenen Polymernetzwerken

Non-Patent Citations (1)

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See also references of EP1056758A2 *

Cited By (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004048398A1 (ja) * 2002-11-28 2004-06-10 Arkray Inc. 核酸の濃縮精製方法および装置
CN1332972C (zh) * 2002-11-28 2007-08-22 爱科来株式会社 浓缩和纯化核酸的方法和装置
EP1568705A1 (de) * 2002-11-28 2005-08-31 Arkray Inc. Verfahren und apparat zum einengen und zur aufreinigung von nukleinsäure
EP1568705A4 (de) * 2002-11-28 2007-04-25 Arkray Inc Verfahren und apparat zum einengen und zur aufreinigung von nukleinsäure
EP1696026A1 (de) * 2003-11-10 2006-08-30 Arkray Inc. Verfahren zur konzentration und reinigung von nukleinsäure sowie vorrichtung dafür
EP1696026A4 (de) * 2003-11-10 2007-05-09 Arkray Inc Verfahren zur konzentration und reinigung von nukleinsäure sowie vorrichtung dafür
WO2005045023A1 (ja) 2003-11-10 2005-05-19 Arkray Inc. 核酸の濃縮精製方法および装置
US9011661B2 (en) 2004-02-02 2015-04-21 Boreal Genomics, Inc. Enrichment of nucleic acid targets
US8133371B2 (en) 2004-02-02 2012-03-13 The University Of British Columbia Scodaphoresis and methods and apparatus for moving and concentrating particles
US11795497B2 (en) 2004-02-02 2023-10-24 Quantum-Si Incorporated Enrichment of nucleic acid targets
US8480871B2 (en) 2004-02-02 2013-07-09 The University Of British Columbia Scodaphoresis and methods and apparatus for moving and concentrating particles
US10975421B2 (en) 2004-02-02 2021-04-13 Quantum-Si Incorporated Enrichment of nucleic acid targets
US8529744B2 (en) 2004-02-02 2013-09-10 Boreal Genomics Corp. Enrichment of nucleic acid targets
US10738351B2 (en) 2004-02-02 2020-08-11 Quantum-Si Incorporated Enrichment of nucleic acid targets
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
US9534304B2 (en) 2004-02-02 2017-01-03 The University Of British Columbia Scodaphoresis and methods and apparatus for moving and concentrating particles
US8182666B2 (en) 2005-02-07 2012-05-22 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
US8608929B2 (en) 2005-02-07 2013-12-17 The University Of British Columbia Apparatus and methods for concentrating and separating particles such as molecules
US8852416B2 (en) 2008-02-01 2014-10-07 The University Of British Columbia Methods and apparatus for particle introduction and recovery
US8475641B2 (en) 2008-02-01 2013-07-02 The University Of British Columbia Methods and apparatus for particle introduction and recovery
US8877028B2 (en) 2009-04-21 2014-11-04 The University Of British Columbia System and methods for detection of particles
US10400266B2 (en) 2011-05-20 2019-09-03 The University Of British Columbia Systems and methods for enhanced SCODA
US9434938B2 (en) 2011-05-20 2016-09-06 The University Of British Columbia Systems and methods for enhanced SCODA
US10829800B2 (en) 2011-05-20 2020-11-10 The University Of British Columbia Systems and methods for enhanced SCODA
US8518228B2 (en) 2011-05-20 2013-08-27 The University Of British Columbia Systems and methods for enhanced SCODA
US9186685B2 (en) 2012-01-13 2015-11-17 The University Of British Columbia Multiple arm apparatus and methods for separation of particles
US9555354B2 (en) 2012-01-13 2017-01-31 The University Of British Columbia Multiple arm apparatus and methods for separation of particles
US9512477B2 (en) 2012-05-04 2016-12-06 Boreal Genomics Inc. Biomarker anaylsis using scodaphoresis
US9340835B2 (en) 2013-03-15 2016-05-17 Boreal Genomics Corp. Method for separating homoduplexed and heteroduplexed nucleic acids
US11130986B2 (en) 2015-05-20 2021-09-28 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins
US11898196B2 (en) 2015-05-20 2024-02-13 Quantum-Si Incorporated Method for isolating target nucleic acid using heteroduplex binding proteins
CN115228262A (zh) * 2022-08-22 2022-10-25 湖南国测生物科技有限公司 实验室密闭空间内核酸污染的立体净化方法
CN115228262B (zh) * 2022-08-22 2023-12-01 湖南国测生物科技有限公司 实验室密闭空间内核酸污染的立体净化方法

Also Published As

Publication number Publication date
US6146511A (en) 2000-11-14
WO1999038874A3 (en) 1999-09-23
DE69928230D1 (de) 2005-12-15
EP1056758B1 (de) 2005-11-09
US6827830B1 (en) 2004-12-07
ATE309260T1 (de) 2005-11-15
AU2237499A (en) 1999-08-16
DE69928230T2 (de) 2006-07-20
EP1056758A2 (de) 2000-12-06
CA2318973A1 (en) 1999-08-05
JP2002502020A (ja) 2002-01-22
AU734170B2 (en) 2001-06-07

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