WO1998030683A2 - Nuclear transfer with differentiated fetal and adult donor cells - Google Patents

Nuclear transfer with differentiated fetal and adult donor cells Download PDF

Info

Publication number
WO1998030683A2
WO1998030683A2 PCT/US1998/000002 US9800002W WO9830683A2 WO 1998030683 A2 WO1998030683 A2 WO 1998030683A2 US 9800002 W US9800002 W US 9800002W WO 9830683 A2 WO9830683 A2 WO 9830683A2
Authority
WO
WIPO (PCT)
Prior art keywords
cells
cell
disease
offspring
differentiated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US1998/000002
Other languages
English (en)
French (fr)
Other versions
WO1998030683A3 (en
Inventor
Steven L. Stice
Jose Cibelli
James Robl
Paul Golueke
F. Abel Ponce De Leon
D. Joseph Jerry
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Massachusetts Boston
University of Massachusetts Amherst
Original Assignee
University of Massachusetts Boston
University of Massachusetts Amherst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=25123802&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=WO1998030683(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority to AU60145/98A priority Critical patent/AU742363C/en
Application filed by University of Massachusetts Boston, University of Massachusetts Amherst filed Critical University of Massachusetts Boston
Priority to BR9806872-5A priority patent/BR9806872A/pt
Priority to JP53095898A priority patent/JP2001512964A/ja
Priority to CN988027941A priority patent/CN1248288B/zh
Priority to NZ336612A priority patent/NZ336612A/xx
Priority to CA2277192A priority patent/CA2277192C/en
Priority to IL13082998A priority patent/IL130829A0/xx
Priority to EP98903349A priority patent/EP1015572B1/en
Priority to DE69837270T priority patent/DE69837270T2/de
Publication of WO1998030683A2 publication Critical patent/WO1998030683A2/en
Publication of WO1998030683A3 publication Critical patent/WO1998030683A3/en
Priority to IL130829A priority patent/IL130829A/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/18—Drugs for disorders of the alimentary tract or the digestive system for pancreatic disorders, e.g. pancreatic enzymes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P13/00—Drugs for disorders of the urinary system
    • A61P13/02—Drugs for disorders of the urinary system of urine or of the urinary tract, e.g. urine acidifiers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/14—Drugs for disorders of the nervous system for treating abnormal movements, e.g. chorea, dyskinesia
    • A61P25/16—Anti-Parkinson drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/04—Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8778—Swine embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/106—Primate
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/02—Cells from transgenic animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/04—Cells produced using nuclear transfer

Definitions

  • the present invention relates to cloning procedures
  • mammalian oocytes of the same species as the donor nuclei.
  • the nuclei are reprogrammed to direct the development of
  • CICM cultured inner cell mass cells
  • the cloned embryos can also be combined with fertilized
  • ES cells can be passaged in an undifferentiated
  • ES cells can be used as an in vi tro model for
  • Mouse ES cells can give rise to germline chimeras when
  • pluripotential embryonic cell lines from pig blastocysts pluripotential embryonic cell lines from pig blastocysts.
  • embryonic fibroblast feeder layers without the use of conditioned medium, and reportedly differentiate into
  • mouse ES cell lines derived from mouse ICMs isolation of mouse ES cell lines derived from mouse ICMs.
  • embryos are totipotent by nuclear transfer and that
  • ICMs isolated and cultured ICMs from 8 or 9 day bovine
  • fibroblast feeder cells instead of bovine uterus epithelial cells
  • animal stem cells which purportedly may be used to obtain
  • ICM inner cell mass
  • bovine ICM cells as donor nuclei
  • bovine pluripotent embryonic cells in nuclear transfer and the production of chimeric
  • granulosa cells adult cells could be used in a bovine
  • nuclei (less than 100), or with in vitro cell lines. It
  • transgenic mammals i.e., embryos, fetuses, offspring.
  • transgenic mammals by which a desired gene is inserted, removed or modified in a differentiated mammalian cell or
  • CICM cells for therapy or diagnosis.
  • CICM cells including human and ungulate CICM cells, for
  • organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation is therapeutically or organ transplantation
  • the CICM cells may be used
  • NT embryos derived from NT embryos, fetuses or offspring, including
  • tissues may be used within the same species or across
  • vi tro e.g. for study of cell differentiation and for
  • Such therapies include by way of example
  • transgenic or genetically engineered CICM cells produced
  • the invention as nuclear donors for nuclear transplantation.
  • the present invention provides a
  • a mammal e.g., embryos, fetuses,
  • the method comprises:
  • NT unit develops into a fetus.
  • the cells, tissues and/or organs of the fetus are provided.
  • the present invention also includes a method of
  • mammals are Also provided by the present invention.
  • the present invention is preferably used for cloning
  • the present invention provides a
  • the method comprises:
  • the present invention provides improved procedures
  • differentiated fetal or adult, mammalian cells are differentiated fetal or adult, mammalian cells.
  • the nuclei are reprogrammed
  • fetuses and offspring or used to produce CICM cells.
  • cloned embryos can also be combined with fertilized
  • present invention uses differentiated cells.
  • transgenic embryonic cell lines could be readily derived
  • the present invention also allows simplification of
  • these cells can be clonally propagated without cytokines
  • transgenic embryos are obtained from the same according to the invention.
  • transgenic cloned embryos can be used to generate transgenic cloned embryos.
  • the present invention eliminates the need to
  • the present invention can also be used to produce
  • CICM cells fetuses or offspring which can be used, for
  • This process can provide a source of "materials" for many
  • hematopoietic chimerism can be used to avoid immunological
  • the present invention provides a
  • NT unit develops into a fetus.
  • the present invention also includes a method of
  • mammals are Also provided by the present invention.
  • the present invention is preferably used
  • the present invention further provides for the use of
  • the present invention provides a
  • the method comprises:
  • the NT units will be cultured to a size
  • Differentiated mammalian cells are those cells which
  • the differentiated cells are those from at least past the
  • the differentiated cells may be derived from ectoderm,
  • Mammalian cells including human cells, may be any suitable mammalian cells.
  • Mammalian cells including human cells, may be any suitable mammalian cells.
  • the present invention include, by way of example,
  • epithelial cells neural cells, epidermal cells,
  • keratinocytes hematopoietic cells, melanocytes
  • chondrocytes chondrocytes, lymphocytes (B and T lymphocytes) .
  • erythrocytes erythrocytes, macrophages, monocytes, mononuclear cells,
  • donor cells i.e., cells useful in
  • the subject invention may be obtained from any cell or
  • Fibroblast cells are an ideal cell type because they
  • Fibroblast cells are differentiated
  • Suitable mammalian sources for oocytes include sheep,
  • the oocytes will be
  • oocytes obtained from ungulates, and most preferably bovine. Methods for isolation of oocytes are well known in
  • bovine oocytes is slaughterhouse materials.
  • This process generally requires collecting
  • prophase I immature oocytes from mammalian ovaries
  • bovine ovaries obtained at a slaughterhouse
  • this period of time is
  • metaphase II oocytes are collected surgically from either
  • human chorionic gonadotropin hCG or similar hormone.
  • stage oocyte as the recipient oocyte because at this stage
  • the oocyte can be or is sufficiently
  • the oocyte activation period generally ranges from
  • immature oocytes may be washed in HEPES
  • HECM buffered hamster embryo culture medium
  • TCM tissue culture medium
  • gonadotropins such as luteinizing hormone (LH) and
  • FSH follicle stimulating hormone
  • the oocytes will preferably be removed and
  • metaphase II oocytes as determined by the presence of
  • Enucleation may be effected by known methods, such as
  • oocytes are either placed in HECM, optionally containing 7.5 micrograms per milliliter cytochalasin B,
  • an embryo culture medium for example an embryo culture medium such as
  • Enucleation may be accomplished microsurgically using
  • the oocytes may then be screened to identify
  • screening may be effected by staining the oocytes with 1
  • culture medium e.g., CRlaa plus 10% serum.
  • the recipient oocytes will be identical to the recipient oocytes.
  • enucleated oocyte will be used to produce NT units
  • Electrofusion is
  • Sendai virus as a fusogenic agent (Graham, lis e Inot .
  • the mammalian cell and oocyte are identical to each other.
  • the mammalian cell and oocyte are identical to each other.
  • suitable medium until activation e.g., CRlaa medium.
  • the NT unit may be activated by known methods. Such as
  • methods include, e.g., culturing the NT unit at sub-
  • activation may be achieved by
  • activation may be effected by
  • the oocyte This will generally be effected by introducing divalent
  • cations into the oocyte cytoplasm e.g., magnesium,
  • strontium barium or calcium, e.g., in the form of an
  • Phosphorylation may be reduced by known methods
  • kinase inhibitors e.g., serine-
  • threonine kinase inhibitors such as 6 -dimethyl -
  • a phosphatase may be inhibited by introduction of a phosphatase into the
  • oocyte e.g., phosphatase 2A and phosphatase 2B.
  • NT activation is effected by
  • the activated NT units may then be cultured in a
  • bovine embryo culture and maintenance include Ham's F-10
  • FCS fetal calf serum
  • oocytes is TCM-199, and 1 to 20% serum supplement
  • medium includes TCM-199 with Earl salts, 10% fetal calf
  • oviduct cells oviduct cells, BRL cells and uterine cells and STO cells.
  • Patent 5,096,822 to Rosenkrans, Jr. et al . which is
  • CR1 contains the nutritional substances necessary
  • CR1 contains hemicalcium L-lactate in amounts ranging
  • Hemicalcium L-lactate is L-lactate with a hemicalcium salt
  • Hemicalcium L-lactate is
  • L-lactate also serves as valuable mineral and energy
  • CR1 medium does not contain serum
  • media comprising animal cells such as oviductal
  • CR1 medium examples include
  • CR1 medium preferably contains the following
  • the activated NT embryos unit are
  • the activated NT units may be any suitable activated NT units.
  • the activated NT units may be any suitable activated NT units.
  • CRlaa medium containing 10% FCS and 6 mg/ml
  • well plates which preferably contain a
  • Suitable confluent feeder layer Suitable confluent feeder layer. Suitable feeder layers
  • fibroblasts include, by way of example, fibroblasts and epithelial cells
  • fibroblasts e.g., fibroblasts and uterine epithelial cells
  • murine e.g., murine
  • the feeder cells comprise mouse
  • the NT units are cultured on the feeder layer until
  • the NT units reach a size suitable for transferring to a
  • NT units will be cultured until at least about 2 to 400
  • the culturing will be effected under suitable conditions, i.e., about 38.5°C and
  • the stage of the NT embryo is in
  • the present invention can also be used to clone
  • the differentiated cells used for donor nuclei have a desired gene inserted, removed or
  • a desired gene from a mammalian cell may be used for
  • the gene may be heterologous. Included is the
  • the present invention can thus be used to provide
  • transgenic or genetically engineered animals and chimeric
  • transgenic and/or chimeric fetuses can be used
  • a feeder layer e.g., irradiated fibroblast
  • the cells used to obtain the stem cells are typically used to obtain the stem cells.
  • portion of the cultured NT unit which is preferably at
  • NT unit may also be used to obtain ES cells and
  • a layer in a suitable growth medium e.g., alpha MEM
  • growth medium is changed as often as necessary to optimize
  • growth e.g., about every 2-3 days.
  • CICM cells or cell lines One skilled in the art can vary
  • transgenic mammalian CICM cells may be produced
  • transgenic NT units can then be used to produce
  • the resultant CICM cells and cell lines preferably
  • cells may be used for cell transplantation therapies.
  • Human CICM cells have application in the treatment of
  • the CICM cells according to the invention will be induced to
  • the subject human CICM human CICM
  • cells may be induced to differentiate into hematopoietic
  • stem cells muscle cells, cardiac muscle cells, liver
  • cartilage cells cartilage cells, epithelial cells, urinary tract
  • hematopoietic stem cells from an embryonic cell line by
  • cell types including hematopoietic cells, muscle, cardiac
  • the subject CICM cells may be used to obtain any
  • human hematopoietic stem cells may be used in medical treatments requiring bone marrow transplantation.
  • late stage cancers such as ovarian cancer and leukemia, as
  • Hematopoietic stem cells can be obtained, e.g.,
  • epithelial cells or lymphocytes with an enucleated
  • Such hematopoietic cells may be used in the
  • a neurological disorder may be fused with an
  • transplantation of such human neural cells include, by transplantation of such human neural cells
  • Parkinson's disease Alzheimer's disease
  • transplanted fetal brain neural cells make the proper connections with surrounding cells and produce dopamine .
  • anti-rejection drugs such as
  • the present invention should eliminate, or at least
  • cell therapy include, by way of example, spinal cord
  • liver diseases i.e., hypercholesterolemia
  • This methodology can be used to replace defective
  • the gene encoding brain derived growth factor may
  • astrocytes have been transfected with
  • retroviral vectors currently used are down-
  • cells include, by way of example, epidermal growth factor,
  • insulin-like growth factor I and II
  • neurotrophin-3 neurotrophin-3/5 , ciliary neurotrophic
  • AFT-1 AFT-1
  • cytokine genes interleukins, interferons, colony stimulating factors, tumor necrosis factors (alpha
  • CICM cells CICM cells, NT fetuses and NT
  • CICM cell fetuses and offspring
  • brain cells across species (xenografts) .
  • xenografts xenografts
  • brain cells For example, brain cells
  • bovine NT fetuses may be used to treat Parkinson's
  • the subject CICM cells preferably human cells
  • CICM cells may be used in drug studies. Further, the subject CICM cells may be used as
  • Fibroblast cells were plated in
  • FCS fetal calf serum
  • the fibroblasts (100 IU/ml) and streptomycin (50 ⁇ l/ml) .
  • tissue was incubated overnight at 10°C in trypsin EDTA
  • FCS fetal calf serum
  • fibroblast cells can be isolated at virtually any time in
  • This procedure can also be used to isolate
  • fibroblasts from other mammals, including mice.
  • a marker gene foreign heterologous DNA
  • embryonic and adult fibroblast cells introduction of a marker gene (foreign heterologous DNA) into embryonic and adult fibroblast cells.
  • the cells were in a single cell suspension. The cells were
  • PBS phosphate buffered saline
  • the reporter gene construct contained the
  • neomycin phosphotransferase fusion gene (beta-GEO) .
  • FCS fetal calf serum
  • G418 400 ⁇ g/ml was added to growth medium for 15

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biotechnology (AREA)
  • Veterinary Medicine (AREA)
  • Wood Science & Technology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Public Health (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Neurology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Neurosurgery (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Diabetes (AREA)
  • Cardiology (AREA)
  • Reproductive Health (AREA)
  • Hospice & Palliative Care (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Gynecology & Obstetrics (AREA)
  • Cell Biology (AREA)
PCT/US1998/000002 1997-01-10 1998-01-05 Nuclear transfer with differentiated fetal and adult donor cells Ceased WO1998030683A2 (en)

Priority Applications (10)

Application Number Priority Date Filing Date Title
IL13082998A IL130829A0 (en) 1997-01-10 1998-01-05 Nuclear transfer with differentiated fetal and adult donor cells
CA2277192A CA2277192C (en) 1997-01-10 1998-01-05 Nuclear transfer with differentiated fetal and adult donor cells
BR9806872-5A BR9806872A (pt) 1997-01-10 1998-01-05 Transferência nuclear com células diferenciadas doadoras fetais e adultas
JP53095898A JP2001512964A (ja) 1997-01-10 1998-01-05 分化した胎仔および成体ドナー細胞による核移植
CN988027941A CN1248288B (zh) 1997-01-10 1998-01-05 用分化胎儿和成年供体细胞进行的核转移
NZ336612A NZ336612A (en) 1997-01-10 1998-01-05 Cloning using donor nuclei from proliferating somatic cells
EP98903349A EP1015572B1 (en) 1997-01-10 1998-01-05 Nuclear transfer with differentiated fetal and adult donor cells
AU60145/98A AU742363C (en) 1997-01-10 1998-01-05 Nuclear transfer with differentiated fetal and adult donor cells
DE69837270T DE69837270T2 (de) 1997-01-10 1998-01-05 Kerntransfer mit differenzierten fötalen und adulten spenderzellen
IL130829A IL130829A (en) 1997-01-10 1999-07-07 Nuclear transfer with differentiated fetal and adult donor cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/781,752 US5945577A (en) 1997-01-10 1997-01-10 Cloning using donor nuclei from proliferating somatic cells
US08/781,752 1997-01-10

Publications (2)

Publication Number Publication Date
WO1998030683A2 true WO1998030683A2 (en) 1998-07-16
WO1998030683A3 WO1998030683A3 (en) 1998-09-17

Family

ID=25123802

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1998/000002 Ceased WO1998030683A2 (en) 1997-01-10 1998-01-05 Nuclear transfer with differentiated fetal and adult donor cells

Country Status (13)

Country Link
US (4) US5945577A (OSRAM)
EP (2) EP1808484A1 (OSRAM)
JP (2) JP2001512964A (OSRAM)
CN (1) CN1248288B (OSRAM)
AT (1) ATE356203T1 (OSRAM)
AU (1) AU742363C (OSRAM)
BR (1) BR9806872A (OSRAM)
CA (1) CA2277192C (OSRAM)
DE (1) DE69837270T2 (OSRAM)
ES (1) ES2284197T3 (OSRAM)
IL (2) IL130829A0 (OSRAM)
NZ (1) NZ336612A (OSRAM)
WO (1) WO1998030683A2 (OSRAM)

Cited By (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999005266A3 (en) * 1997-07-26 1999-04-15 Wisconsin Alumni Res Found Trans-species nuclear transfer
WO1999036510A1 (de) * 1998-01-16 1999-07-22 Agrobiogen Gmbh Effizienter kerntransfer mit fetalen fibroblasten
WO1999046982A1 (en) * 1998-03-16 1999-09-23 Relag Pty Ltd Porcine nuclear transfer
WO1999053025A1 (en) * 1998-04-15 1999-10-21 Monash University Method of nuclear transfer
WO1999053751A1 (en) * 1998-04-20 1999-10-28 Consorzio Incremento Zootecnico S.R.L. Source of nuclei for nuclear transfer
WO1999063061A1 (en) * 1998-06-02 1999-12-09 Biotechnology And Biological Sciences Research Council Es cell lines from somatic cells via embryo reconstruction and selective ablation
WO1999037143A3 (en) * 1998-01-21 2000-02-03 Univ Hawaii Full term development of animals from enucleated oocytes reconstituted with adult somatic cell nuclei
WO2000008924A1 (en) * 1998-08-11 2000-02-24 Perry Anthony C F Method of performing transgenesis
WO2000012682A1 (en) * 1998-09-01 2000-03-09 Wisconsin Alumni Research Foundation Primate embryonic stem cells with compatible histocompatibility genes
WO2000051424A3 (en) * 1999-03-04 2001-03-22 Ppl Therapeutics Scotland Ltd Genetic modification of somatic cells and uses thereof
WO2001035734A1 (en) * 1999-11-19 2001-05-25 Korea Research Institute Of Bioscience And Biotechnolgoy An improved method for production of porcine clone embryos via somatic cell nuclear transfer
EP1045635A4 (en) * 1998-01-08 2001-10-24 Univ Massachusetts CLONING USING DIFFERENTIATED FETAL CELLS AND ADULT CELLS
WO2002009507A1 (de) * 2000-07-27 2002-02-07 Apogene Gmbh & Co. Kg Somatischer klonierungs-gentransfer zur produktion von rekombinanten proteinen, zellen und organen
WO2001053473A3 (en) * 2000-01-22 2002-05-30 Univ Edinburgh Methods for amplifying genetic material and uses thereof
EP1238584A1 (en) * 2001-03-07 2002-09-11 Director General of National Agricultural Research Organization Method for producing livestock individuals from cells of established cell line
AU753209B2 (en) * 1999-06-30 2002-10-10 Seoul National University Industry Foundation Method for producing cloned tigers by employing inter-species nuclear transplantation technique
AU753207B2 (en) * 1999-06-30 2002-10-10 Seoul National University Industry Foundation Method for producing cloned cows
JP2003512052A (ja) * 1999-10-15 2003-04-02 アドバンスド セル テクノロジー、インコーポレイテッド 分化前駆細胞及び系統欠失胚性幹細胞の産生方法
JP2003512848A (ja) * 1999-11-02 2003-04-08 ユニバーシティ オブ マサチューセッツ、アズ リプリゼンティッド バイ イッツ アマースト キャンパス 遺伝的診断、修飾および増殖のための一倍体ゲノムの使用
WO2001068831A3 (en) * 2000-03-15 2003-08-28 Univ Georgia Res Found Effective nuclear reprogramming in mammals
WO2003085105A1 (en) * 2002-04-01 2003-10-16 Gtc Biotherapeutics, Inc. A method for selecting cell lines to be used for nuclear transfer in mammalian species
EP1692936A1 (en) 2000-10-03 2006-08-23 GIE-CERBM, Centre Europeen de Recherche en Biologie et en Médecine Method for targeted conditional DNA recombination in mice using the cre-ert2 fusion protein
US7129084B2 (en) 2000-08-03 2006-10-31 Therapeutic Human Polyclonals, Inc. Production of humanized antibodies in transgenic animals
EP1127112B1 (en) * 1998-11-02 2007-03-21 Trustees Of Tufts College Methods for cloning animals
US7249569B2 (en) 2001-08-13 2007-07-31 Embrex, Inc. Methods for injecting avian eggs
US7309811B2 (en) 2001-02-23 2007-12-18 Elan Pharmaceuticals, Inc Transgenic mice knockouts of BACE-1
US7414170B2 (en) 1999-11-19 2008-08-19 Kirin Beer Kabushiki Kaisha Transgenic bovines capable of human antibody production
US7420099B2 (en) 2004-04-22 2008-09-02 Kirin Holdings Kabushiki Kaisha Transgenic animals and uses thereof
US7429690B2 (en) 2002-11-08 2008-09-30 Kirin Holdings Kabushiki Kaisha Transgenic bovines having reduced prion protein production
US7491867B2 (en) 1999-11-19 2009-02-17 Kyowa Hakko Kirin Co., Ltd. Expression of xenogenous (human) immunoglobulins in cloned, transgenic ungulates
EP2138583A1 (en) 2000-12-22 2009-12-30 Institut National De La Recherche Agronomique Position-independent and tissue specific expression of a transgene in milk of transgenic animals
EP2267153A1 (en) 2009-05-26 2010-12-29 Université Claude Bernard Lyon 1 Identification of netrin-1 receptor unc5c gene mutation in solid cancers
WO2012020077A1 (en) 2010-08-11 2012-02-16 Inserm (Institut Mational De La Santé Et De La Recherche Médicale) Methods of growing an embryo to a blastocyst stage of development
WO2012112586A1 (en) 2011-02-14 2012-08-23 Revivicor, Inc. Genetically modified pigs for xenotransplantation of vascularized xenografts and derivatives thereof
US8796021B2 (en) 2007-02-23 2014-08-05 Advanced Cell Technology, Inc. Blastomere culture to produce mammalian embryonic stem cells
CN104488853A (zh) * 2015-01-12 2015-04-08 中国农业大学 一种牛胚胎玻璃化冷冻甩管解冻和直接移植方法
EP2987498A1 (en) 2008-05-02 2016-02-24 Laboratoire Français du Fractionnement et des Biotechnologies Treatment of bleeding with low half-life fibrinogen
EP3138403A1 (en) 2005-08-09 2017-03-08 Revivicor, Inc. Transgenic ungulates expressing ctla4-ig and uses thereof
US10501723B2 (en) 2005-08-03 2019-12-10 Astellas Institute For Regenerative Medicine Methods of reprogramming animal somatic cells
US10865383B2 (en) 2011-07-12 2020-12-15 Lineage Cell Therapeutics, Inc. Methods and formulations for orthopedic cell therapy
WO2022109316A1 (en) 2020-11-20 2022-05-27 Revivicor, Inc. Multi-transgenic pigs with growth hormone receptor knockout for xenotransplantation
WO2023044100A1 (en) 2021-09-20 2023-03-23 Revivicor, Inc. Multitran scenic pigs comprising ten genetic modifications for xenotransplantation

Families Citing this family (178)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9517780D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
GB9517779D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US5945577A (en) * 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells
US20090092588A1 (en) * 1997-01-10 2009-04-09 University Of Massachusetts As Represented By Its Amherst Campus Cloned ungulate embryos and animals, use of cells, tissues and organs thereof for transplantation therapies including parkinson's disease
US6235969B1 (en) * 1997-01-10 2001-05-22 University Of Massachusetts Cloning pigs using donor nuclei from non-quiescent differentiated cells
US6011197A (en) * 1997-03-06 2000-01-04 Infigen, Inc. Method of cloning bovines using reprogrammed non-embryonic bovine cells
JP2002512510A (ja) * 1997-03-06 2002-04-23 インフィジェン・インコーポレーテッド 動物のクローニング方法
JP2001509361A (ja) 1997-07-03 2001-07-24 ユニヴァーシティー オヴ マサチューセッツ 分化した無血清飢餓細胞からの供与核を用いるクローニング
US20030177513A1 (en) * 1998-11-02 2003-09-18 Yann Echelard Transgenic and cloned mammals
US6580017B1 (en) * 1998-11-02 2003-06-17 Genzyme Transgenics Corporation Methods of reconstructed goat embryo transfer
US6781030B1 (en) 1998-11-02 2004-08-24 Trustee Of Tufts College, Ballou Hall Methods for cloning mammals using telophase oocytes
US6700037B2 (en) 1998-11-24 2004-03-02 Infigen, Inc. Method of cloning porcine animals
US7531715B1 (en) 1999-01-13 2009-05-12 Ppl Therapeutics (Scotland) Double nuclear transfer method and results thereof
US20020012660A1 (en) * 1999-03-04 2002-01-31 Alan Colman Method of preparing a somatic cells for nuclear transfer
ATE394029T1 (de) * 1999-05-06 2008-05-15 Stem Cell Sciences Australia P Verfahren der zellreprogrammierung durch herstellung eines heterokaryons
EP1109890A4 (en) * 1999-06-30 2004-12-29 Hwang Woo Suk PROCESS FOR OBTAINING CLONED HUMAN EMBRYOS USING INTER-SPECIES TRANSPLANTATION TECHNIQUES OF CORES
US20050255596A1 (en) * 1999-09-07 2005-11-17 West Michael D Methods of repairing tandemly repeated DNA sequences and extending cell life-span nuclear transfer
EP1214443A4 (en) * 1999-09-07 2003-08-27 Advanced Cell Tech Inc RESTORATION OF THE TELOMER AND EXTENSION OF THE CELL LIFETIME IN CLONED ANIMALS FROM SENESCENT SOMATIC CELLS
US20040180430A1 (en) * 1999-09-07 2004-09-16 West Michael D. Methods of restoring telomere length and extending cell lifespan using nuclear transfer
EP1241931A4 (en) * 1999-09-13 2004-12-29 Univ Massachusetts CLONING PIGS USING DONORATION CELLS OR NUCLEAR CELLS OF DIFFERENTIATED CELLS AND PRODUCING PLURIPOTENT PIGS
EP2275559A3 (en) 1999-09-28 2011-03-23 Shire Human Genetic Therapies, Inc. Optimized messenger RNA
WO2002064799A2 (en) 1999-09-28 2002-08-22 Transkaryotic Therapies, Inc. Optimized messenger rna
EP1230342A4 (en) 1999-10-28 2003-04-09 Univ Massachusetts GYNOGENETIC OR ANDROGENETIC PRODUCTION OF PLURIPOTENT CELLS AND CELL LINES AND THEIR USE IN THE PRODUCTION OF DIFFERENTIATED CELLS AND TISSUES
EP1242591A1 (en) * 1999-10-29 2002-09-25 Nexia Biotechnologies, Inc. Production of transgenic animals using nuclear transfer and oocytes recovered by lopu
US7253334B2 (en) * 2000-12-22 2007-08-07 Aurox, Llc Methods for cloning non-human mammals using reprogrammed donor chromatin or donor cells
AU1777301A (en) * 1999-11-19 2001-05-30 Hematech, Llc Production of ungulates, preferably bovines that produce human immunoglobulins
US7820878B2 (en) * 1999-11-19 2010-10-26 Kyowa Hakko Kirin Co., Ltd. Production of ungulates, preferably bovines that produce human immunoglobulins
CN1293188C (zh) * 2000-01-04 2007-01-03 康涅狄格州大学 一种将长期培养的可包括人工诱导基因改变的雌或雄体细胞的细胞核转移到去核受体细胞中实现靶向基因改变的克隆动物的方法
US20060174358A1 (en) * 2001-01-04 2006-08-03 Xiangzhong Yang Method for cloning animals with targetted genetic alterations by transfer of long-term cultured male or female somatic cell nuclei, comprising artificially-induced genetic alterations, to enucleated recipient cells
AU2766501A (en) 2000-01-04 2001-07-16 University Of Connecticut, The Oocyte vitrification technique
US6635802B1 (en) * 2000-01-10 2003-10-21 The Texas A&M University System Nuclear transfer using cells cultured in serum starvation media containing apoptosis inhibitors
AU2001295194A1 (en) * 2000-05-04 2001-11-12 Biotime, Inc. Methods and compositions for producing donor cells
AU2001261968A1 (en) * 2000-05-25 2001-12-03 Oregon Health Sciences University Pt32 sperm protein, sperm c-yes, oocyte cytoplasmic c-yes, and uses thereof
US20030032180A1 (en) * 2000-07-29 2003-02-13 Strelchenko Larissa V. Reconstituted cell lines prepared by nuclear transfer between differentiated cells
US20030172394A1 (en) * 2001-08-01 2003-09-11 Holzer George L Selection and cloning methods
US20040029825A1 (en) * 2001-10-03 2004-02-12 Davies Christopher J Methods of minimizing immunological rejection of a nuclear transfer fetus
WO2002102997A2 (en) * 2000-11-30 2002-12-27 Stemron Inc. Isolated homozygous stem cells differentiated cells derived therefrom and materials and methods for making and using same
US20030027331A1 (en) * 2000-11-30 2003-02-06 Yan Wen Liang Isolated homozygous stem cells, differentiated cells derived therefrom, and materials and methods for making and using same
US7491534B2 (en) * 2000-12-22 2009-02-17 Kirin Holdings Kabushiki Kaisha Methods for altering cell fate to generate T-cells specific for an antigen of interest
US20020142397A1 (en) * 2000-12-22 2002-10-03 Philippe Collas Methods for altering cell fate
NZ526888A (en) * 2001-01-02 2008-07-31 Stemron Inc Isolated homozygous stem (HS) cells and the discovery that these cells may be selected for immunotype and genotype, therefore being useful for diagnosis, transplantation and/or treatment
AUPR247901A0 (en) * 2001-01-10 2001-02-01 Garelag Pty Ltd Activation of nuclear transfer embryos
CA2435857A1 (en) 2001-01-30 2002-09-26 Rebholtz, Amy K. Methods for embryo recipient management
WO2002062131A2 (en) * 2001-02-02 2002-08-15 Infigen, Inc. Method of cloning transgenic mammalian animals using pseudonuclei
WO2002073187A1 (en) * 2001-03-09 2002-09-19 Advanced Cell Technology, Inc. Method for generating replacement cells and/or tissues
WO2002073188A1 (en) * 2001-03-13 2002-09-19 Advanced Cell Technology, Inc. Method for generating replacement cells and/or tissues
WO2002078449A2 (en) * 2001-04-02 2002-10-10 Advanced Cell Technology, Inc. Method for facilitating the production of differentiated cell types and tissues from embryonic and adult pluripotent and multipotent cells
AUPR446701A0 (en) 2001-04-18 2001-05-17 Gene Stream Pty Ltd Transgenic mammals for pharmacological and toxicological studies
US20030092174A1 (en) * 2001-05-14 2003-05-15 Monika Liljedahl Tissues or organs for use in xenotransplantation
US20030153044A1 (en) * 2001-05-14 2003-08-14 Monika Liljedahl Tissues or organs for use in xenotransplantation
WO2002103350A1 (en) * 2001-06-14 2002-12-27 Infigen, Inc. Methods for cloning mammals using remodeling factors
AU2002326353A1 (en) * 2001-07-09 2003-01-29 Alexion Pharmaceuticals Inc. Cloned non-human mammals from contact inhibited donor cells
US20060083722A1 (en) * 2001-07-18 2006-04-20 Advanced Cell Technology, Inc Methods and compositions for cell therapy
US20030017587A1 (en) * 2001-07-18 2003-01-23 Rader William C. Embryonic stem cells, clinical applications and methods for expanding in vitro
US20030051264A1 (en) * 2001-07-31 2003-03-13 Monika Liljedahl Genetically modified cows having reduced susceptibility to mad cow disease
US20030211603A1 (en) * 2001-08-14 2003-11-13 Earp David J. Reprogramming cells for enhanced differentiation capacity using pluripotent stem cells
IL160351A0 (en) * 2001-08-23 2004-07-25 Reliance Life Sciences Pvt Ltd ISOLATION OF INNER CELL MASS FOR THE ESTABLISHMENT OF HUMAN EMBRYONIC STEM CELL (hESC) LINES
JP2005500847A (ja) * 2001-08-24 2005-01-13 アドバンスド セル テクノロジー、インコーポレイテッド 分化誘導薬の同定のためのスクリーニング検定及び細胞治療用の分化細胞の調製
US7527974B2 (en) * 2001-11-06 2009-05-05 Shanghai Second Medical University Embryonic stem cells derived from human somatic cell—rabbit oocyte NT units
AU2002361601A1 (en) * 2001-11-06 2003-05-19 Mark C. Fishman Stem and progenitor cell capture for tissue regeneration
MXPA04005010A (es) * 2001-11-26 2005-04-08 Advanced Cell Tech Inc METODO PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS METODOS PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS HUMANOS REPROGRAMADOS Y CELULAS DEL TALLO HUMANAS AUTOLOGAS E ISOGENICAS.
US7018819B2 (en) * 2001-11-30 2006-03-28 Cellectricon Ab Method and apparatus for manipulation of cells and cell-like structures focused electric fields in microfludic systems and use thereof
US20030113910A1 (en) * 2001-12-18 2003-06-19 Mike Levanduski Pluripotent stem cells derived without the use of embryos or fetal tissue
US20040133931A1 (en) * 2003-01-08 2004-07-08 Gavin William G. Method and system for fusion and activation following nuclear transfer in reconstructed embryos
JP2005515782A (ja) * 2002-01-11 2005-06-02 ジーティーシー バイオセラピューティックス インコーポレイテッド 再構築された胚へ核を移植した後の融合および活性化のための方法およびシステム
WO2003060083A2 (en) * 2002-01-15 2003-07-24 Advanced Cell Technology, Inc. Cloning b and t lymphocytes
US20030134422A1 (en) * 2002-01-16 2003-07-17 Sayre Chauncey Bigelow Stem cell maturation for all tissue lines
US20050170506A1 (en) * 2002-01-16 2005-08-04 Primegen Biotech Llc Therapeutic reprogramming, hybrid stem cells and maturation
US20050090004A1 (en) * 2003-01-16 2005-04-28 Sayre Chauncey B. Stem cell maturation for all tissue lines
AU2003251286B2 (en) * 2002-01-23 2007-08-16 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
US6991066B2 (en) * 2002-02-01 2006-01-31 International Business Machines Corporation Customized self-checkout system
US20030180268A1 (en) * 2002-02-05 2003-09-25 Anthony Atala Tissue engineered construct for supplementing or replacing a damaged organ
US20030229909A1 (en) * 2002-02-13 2003-12-11 The Texas A&M University System Cloning cats by nuclear transplantation
AU2003217569A1 (en) * 2002-02-21 2003-09-09 Advanced Cell Technology, Inc. Pluripotent cells comprising allogenic nucleus and mitochondria
US7736892B2 (en) 2002-02-25 2010-06-15 Kansas State University Research Foundation Cultures, products and methods using umbilical cord matrix cells
US20030161818A1 (en) * 2002-02-25 2003-08-28 Kansas State University Research Foundation Cultures, products and methods using stem cells
US20030232410A1 (en) * 2002-03-21 2003-12-18 Monika Liljedahl Methods and compositions for using zinc finger endonucleases to enhance homologous recombination
US20030217378A1 (en) * 2002-04-05 2003-11-20 The University Of Georgia Research Foundation, Inc Cloning using rapidly matured oocytes
AU2003232003B2 (en) * 2002-04-18 2006-05-11 Idaho Research Foundation Activation of equine oocytes
WO2003090526A1 (en) * 2002-04-24 2003-11-06 University Of Georgia Research Foundation Production of cloned offspring from cooled carcasses
AU2003295309A1 (en) * 2002-04-30 2004-04-19 Nucleotech, Llc Methods for altering cell fate to generate t-cells specific for an antigen of interest
US20040091936A1 (en) 2002-05-24 2004-05-13 Michael West Bank of stem cells for producing cells for transplantation having HLA antigens matching those of transplant recipients, and methods for making and using such a stem cell bank
EP1513928B1 (en) 2002-05-24 2019-08-21 Advanced Cell Technology, Inc. A bank of stem cells for producing cells for transplantation having hla antigens matching those of transplant recipients, and methods for making and using such a stem cell bank
US7612250B2 (en) 2002-07-29 2009-11-03 Trustees Of Tufts College Nuclear transfer embryo formation method
WO2004013299A2 (en) * 2002-08-02 2004-02-12 The General Hospital Corporation Methods for the production of cells and mammals with desired genetic modifications
JP2006502748A (ja) * 2002-09-05 2006-01-26 カリフォルニア インスティテュート オブ テクノロジー 遺伝子ターゲッティングを誘発するキメラヌクレアーゼの使用方法
US20050034175A1 (en) * 2002-09-09 2005-02-10 Latif Zuhair A. Immune cloning and uses thereof
AU2003286442A1 (en) * 2002-10-16 2004-05-04 Advanced Cell Technology, Inc. Methods using gene trapped stem cells for marking pathways of stem cell differentiation and making and isolating differentiated cells
US20040077077A1 (en) * 2002-10-18 2004-04-22 Xiangzhong Yang Novel methods for the production of cloned mammals, mammals cloned according to the methods, and methods of use of same
KR100479638B1 (ko) * 2002-10-21 2005-03-31 주식회사 마크로젠 전기자극 및 막항원 표시자를 이용한 핵이식용 공여체 준비방법 및 이를 이용한 복제동물의 생산방법
US7627373B2 (en) 2002-11-30 2009-12-01 Cardiac Pacemakers, Inc. Method and apparatus for cell and electrical therapy of living tissue
CN1683524A (zh) * 2003-04-08 2005-10-19 东南大学 用体细胞核移植技术制备移核胚胎干细胞的方法
US20060037086A1 (en) * 2003-04-09 2006-02-16 Schatten Gerald P Methods for correcting mitotic spindle defects and optimizing preimplantation embryonic developmental rates associated with somatic cell nuclear transfer in animals
KR20060057528A (ko) * 2003-04-09 2006-05-26 마제-위민스 헬스 코퍼레이션 동물에서 체세포 핵 전이와 연관된 유사분열 방추 결함을교정하는 방법
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
US20120196370A1 (en) 2010-12-03 2012-08-02 Fyodor Urnov Methods and compositions for targeted genomic deletion
US8409861B2 (en) 2003-08-08 2013-04-02 Sangamo Biosciences, Inc. Targeted deletion of cellular DNA sequences
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US7550263B2 (en) * 2003-09-05 2009-06-23 Gtc Biotherapeutics, Inc. Method for the production of fusion proteins in transgenic mammal milk
US7972854B2 (en) 2004-02-05 2011-07-05 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US20050186608A1 (en) * 2004-02-19 2005-08-25 Olsen Byron V. Method for the production of transgenic proteins useful in the treatment of obesity and diabetes
US7840263B2 (en) * 2004-02-27 2010-11-23 Cardiac Pacemakers, Inc. Method and apparatus for device controlled gene expression
US20050197496A1 (en) * 2004-03-04 2005-09-08 Gtc Biotherapeutics, Inc. Methods of protein fractionation using high performance tangential flow filtration
US7764995B2 (en) 2004-06-07 2010-07-27 Cardiac Pacemakers, Inc. Method and apparatus to modulate cellular regeneration post myocardial infarct
US7828711B2 (en) * 2004-08-16 2010-11-09 Cardiac Pacemakers, Inc. Method and apparatus for modulating cellular growth and regeneration using ventricular assist device
JP2006081542A (ja) * 2004-08-18 2006-03-30 Institute Of Physical & Chemical Research クローン哺乳動物の作成方法
WO2006018316A1 (en) * 2004-08-20 2006-02-23 Ingenium Pharmaceuticals Ag Methods for the production of improved live stocks and disease models for therapeutic research
US20060121004A1 (en) * 2004-12-07 2006-06-08 Yann Echelard Methods of reducing the incidence of rejection in tissue transplantation through the use of recombinant human antithrombin
US20060123500A1 (en) * 2004-12-07 2006-06-08 Gtc Biotherapeutics, Inc. Methods of prescreening cells for nuclear transfer procedures
US20060130159A1 (en) * 2004-12-09 2006-06-15 Nick Masiello Method of purifying recombinant MSP 1-42 derived from Plasmodium falciparum
US7981065B2 (en) 2004-12-20 2011-07-19 Cardiac Pacemakers, Inc. Lead electrode incorporating extracellular matrix
US8060219B2 (en) 2004-12-20 2011-11-15 Cardiac Pacemakers, Inc. Epicardial patch including isolated extracellular matrix with pacing electrodes
KR100733012B1 (ko) 2005-07-26 2007-06-28 재단법인서울대학교산학협력재단 복제된 개과 동물 및 이의 생산 방법
US7732202B2 (en) 2005-10-21 2010-06-08 International Stem Cell Corporation Oxygen tension for the parthenogenic activation of human oocytes for the production of human embryonic stem cells
ES2417147T3 (es) 2005-10-21 2013-08-06 Revo Biologics, Inc. Anticuerpos con actividad de citotoxicidad celular dependiente de anticuerpos mejorada, métodos para su producción y uso
US7531632B2 (en) * 2006-02-16 2009-05-12 Gtc Biotherapeutics, Inc. Clarification of transgenic milk using depth filtration
CA2658813A1 (en) * 2006-07-24 2008-01-31 International Stem Cell Corporation Synthetic cornea from retinal stem cells
ES2378407T3 (es) 2006-09-01 2012-04-12 Therapeutic Human Polyclonals, Inc. Aumento de la expresión de inmunoglobulina humana o humanizada en animales transgénicos no humanos
US20100205678A1 (en) * 2007-01-05 2010-08-12 Overstrom Eric W Oocyte spindle-associated factors improve somatic cell cloning
KR100829426B1 (ko) * 2007-01-17 2008-05-15 재단법인서울대학교산학협력재단 복제개의 생산 방법
US20080222745A1 (en) * 2007-03-07 2008-09-11 Utah State University Colcemid-Treatment of Oocytes to enhance Nuclear Transfer Cloning
WO2008124142A1 (en) 2007-04-06 2008-10-16 International Stem Cell Corporation Patient-specific stem cell lines derived from human parthenogenetic blastocysts
KR100868245B1 (ko) * 2007-05-29 2008-11-11 강원대학교산학협력단 데메콜친 처리에 의한 효율적인 복제 동물의 생산방법
WO2009066934A2 (en) * 2007-11-19 2009-05-28 Seoul National University Industry Foundation Methods for improvement of birth rates in canidae on somatic cell nuclear transfer
WO2009088189A2 (ko) 2008-01-04 2009-07-16 Seoul National University Industry Foundation 형질전환된 복제개의 생산방법
US20120321595A1 (en) 2009-11-09 2012-12-20 The Brigham And Women's Hospital, Inc. Treatment of heart disease
PL2613776T3 (pl) 2010-09-01 2021-01-11 Thomas Jefferson University Kompozycja oraz sposób naprawy i regeneracji mięśni
CA2812194C (en) 2010-09-17 2022-12-13 President And Fellows Of Harvard College Functional genomics assay for characterizing pluripotent stem cell utility and safety
SI2709445T1 (sl) 2011-05-16 2018-11-30 The Curators Of The University Of Missouri Živali, odporne proti virusu prašičjega reprodukcijskega in respiratornega sindroma
CA2844435A1 (en) 2011-08-10 2013-02-14 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Highly galactosylated antibodies
EP2599859A1 (en) 2011-11-30 2013-06-05 IMBA-Institut für Molekulare Biotechnologie GmbH Haploid cells
AU2012345676B2 (en) 2011-12-01 2018-10-04 New York Stem Cell Foundation, Inc. Automated system for producing induced pluripotent stem cells or differentiated cells
EP2802647B1 (en) 2012-01-13 2020-10-28 The General Hospital Corporation Isolated human lung progenitor cells and uses thereof
CN104411815B (zh) 2012-04-30 2017-11-28 大学健康网络 用于从hPSC生成胰腺祖细胞和功能性β细胞的方法和组合物
JP6444307B2 (ja) 2012-09-25 2018-12-26 イエール ユニバーシティ 胚体内胚葉を介したヒト肺胞ｉｉ型へのヒトｉｐｓ細胞の分化
MX2015010429A (es) 2013-02-13 2016-05-16 Lab Francais Du Fractionnement Cetuximab con glicosilación modificada y sus usos.
BR112015019348A2 (pt) 2013-02-13 2017-08-22 Lab Francais Du Fractionnement Métodos para produção de proteína com glicosilação modificada e com sialilação aumentada, para aumentar a atividade de sialil transferase na glândula mamária e para produzir sialil transferase, proteína com glicosilação modificada ou proteína com sialilação aumentada, composição, sialil transferase, mamífero transgênico, e, célula epitelial mamária
CN105308071A (zh) 2013-02-13 2016-02-03 法国化学与生物科技实验室 高度半乳糖基化的抗her2抗体及其用途
AU2014217561B2 (en) 2013-02-13 2018-11-15 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Highly galactosylated anti-TNF-alpha antibodies and uses thereof
US9993504B2 (en) 2013-04-05 2018-06-12 University Health Network Methods and compositions for generating chondrocyte lineage cells and/or cartilage like tissue
JP6697380B2 (ja) 2013-06-10 2020-05-20 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 多能性幹細胞の有用性および安全性を特徴付けるための初期発生ゲノムアッセイ
KR102580225B1 (ko) 2013-06-11 2023-09-20 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 SC-β 세포 및 조성물 그리고 그 생성 방법
WO2014201254A1 (en) 2013-06-12 2014-12-18 University Of Washington Through Its Center For Commercialization Methods for maturing cardiomyocytes and uses thereof
WO2015023720A1 (en) 2013-08-16 2015-02-19 Yale University Epithelial cell differentiation of human mesenchymal stromal cells
AU2014321101B2 (en) 2013-09-13 2020-12-03 University Health Network Methods and compositions for generating epicardium cells
WO2015069736A1 (en) 2013-11-08 2015-05-14 The Mclean Hospital Corporation METHODS FOR EFFICIENT GENERATION OF GABAergic INTERNEURONS FROM PLURIPOTENT STEM CELLS
EP3096713B1 (en) 2014-01-23 2022-09-21 President and Fellows of Harvard College Engineered polymeric valves, tubular structures and sheets
CA2950241A1 (en) 2014-06-02 2015-12-10 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Production of fc fragments
DK3183337T3 (en) 2014-08-22 2019-04-01 Procella Therapeutics Ab Use of Jagged 1 / Frizzled 4 as cell surface marker for isolation of human cardiac ventricular progenitor cells
US10596200B2 (en) 2014-08-22 2020-03-24 Procella Therapeutics Ab Use of LIFR or FGFR3 as a cell surface marker for isolating human cardiac ventricular progenitor cells
KR102473092B1 (ko) 2014-09-15 2022-12-01 칠드런'즈 메디컬 센터 코포레이션 히스톤 h3-리신 트리메틸화를 제거함으로써 체세포 핵 이식(scnt) 효율을 증가시키는 방법 및 조성물
WO2016131137A1 (en) 2015-02-17 2016-08-25 University Health Network Methods for making and using sinoatrial node-like pacemaker cardiomyocytes and ventricular-like cardiomyocytes
JP6929527B2 (ja) * 2015-03-04 2021-09-01 株式会社ポル・メド・テック 安定した表現型を示す疾患モデルブタおよびその作製方法
WO2016178087A1 (en) 2015-05-04 2016-11-10 Biosourcing Sa Transgenic production of chorionic gonadotropin
BR112017023503A2 (pt) 2015-05-04 2018-07-31 Biosourcing Sa método, composição, e, mamífero transgênico não humano.
FR3038517B1 (fr) 2015-07-06 2020-02-28 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Utilisation de fragments fc modifies en immunotherapie
RU2018107842A (ru) 2015-08-06 2019-09-09 Дзе Кьюрейторз Оф Дзе Юниверсити Оф Миссури Устойчивые к патогенам животные, имеющие модифицированные гены cd163
WO2017172086A1 (en) 2016-02-19 2017-10-05 Leung Chuen Yan Genetic markers for engraftment of human cardiac ventricular progenitor cells
US11534466B2 (en) 2016-03-09 2022-12-27 Aal Scientifics, Inc. Pancreatic stem cells and uses thereof
WO2017202814A1 (en) 2016-05-24 2017-11-30 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods and pharmaceutical compositions for the treatment of neuropathological disorders characterized by a loss of cortical neurons
CA3042382A1 (en) 2016-11-02 2018-05-11 Aal Scientifics, Inc. Non-mesenchymal human lung stem cells and methods of their use for treating respiratory diseases
US10508263B2 (en) 2016-11-29 2019-12-17 Procella Therapeutics Ab Methods for isolating human cardiac ventricular progenitor cells
WO2019038587A1 (en) 2017-08-23 2019-02-28 Procella Therapeutics Ab USE OF NEUROPILIN-1 (NRP1) AS A CELL SURFACE MARKER FOR ISOLATING HUMAN CARDIAC VENTRICULAR PROGENITOR CELLS
US11618884B2 (en) 2017-11-14 2023-04-04 Inserm (Institut National De La Sante Et De La Recherche Medicale) Regulatory T cells genetically modified for the lymphotoxin alpha gene and uses thereof
WO2019116096A1 (en) 2017-12-15 2019-06-20 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Production of fc fragments
US11160260B2 (en) 2018-04-17 2021-11-02 The Curators Of The University Of Missouri Methods for protecting porcine fetuses from infection with porcine reproductive and respiratory syndrome virus (PRRSV)
WO2019227198A1 (en) 2018-05-31 2019-12-05 University Health Network (Uhn) Methods and compositions comprising tankyrase inhibitors for generating insulin producing cells
AU2019335025A1 (en) 2018-09-07 2021-04-08 Hebecell Corporation Methods and compositions for retinal neuron generation in carrier-free 3D sphere suspension culture
WO2020053661A1 (en) 2018-09-13 2020-03-19 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Methods of purifying antibodies from the milk of transgenic non-human mammals comprising the use of chitosan
EP3969569A1 (en) 2019-05-14 2022-03-23 INSERM (Institut National de la Santé et de la Recherche Médicale) Regulatory t cells targeted by lymphotoxin alpha blocking agent and uses thereof
EP4041867A1 (en) 2019-10-09 2022-08-17 Institut National de la Santé et de la Recherche Médicale (INSERM) T cells modified to express mutated cxcr4 or partially deleted and uses thereof
WO2023178239A1 (en) 2022-03-16 2023-09-21 The Children's Medical Center Corporation Hpsc-derived articular chondrocyte compositions, systems and methods of use thereof
US20230330264A1 (en) 2022-04-15 2023-10-19 Smartcella Solutions Ab COMPOSITIONS AND METHODS FOR EXOSOME-MEDIATED DELIVERY OF mRNA AGENTS
CN119522044A (zh) 2022-05-26 2025-02-25 藤得食品股份有限公司 基于植物的肉丝制品及其生产方法
US20240090531A1 (en) 2022-09-16 2024-03-21 Tender Food, Inc. Plant and animal cell blended meat products and methods of producing the same
JP2026504725A (ja) 2022-12-16 2026-02-09 テンダー フード，インコーポレイテッド 植物ベースの家禽製品及びその製造方法
WO2025217332A1 (en) 2024-04-10 2025-10-16 Tender Food, Inc. Apparatus for production of food-grade fibers and methods of operating the same
WO2026083330A1 (en) 2024-10-17 2026-04-23 Roslin Cell Therapies Limited Compositions and methods for reprogramming of cells

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4994384A (en) * 1986-12-31 1991-02-19 W. R. Grace & Co.-Conn. Multiplying bovine embryos
US5057420A (en) * 1987-06-05 1991-10-15 Granada Biosciences, Inc. Bovine nuclear transplantation
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
AU1439295A (en) * 1993-12-17 1995-07-03 Abs Global, Inc. Ungulate preblastocyst derived embryonic stem cells and use thereof to produce cloned transgenic and chimeric ungulates
AU1595195A (en) * 1993-12-23 1995-07-10 Abs Global, Inc. Embryonic stem cells as nuclear donors and nuclear transfer techniques to produce chimeric and transgenic animals
AUPM621394A0 (en) * 1994-06-10 1994-07-07 Mitchell, John Cathode connection for aluminium smelter pot
JP3790268B2 (ja) * 1994-06-15 2006-06-28 明治乳業株式会社 有蹄類のｅｇ細胞
GB9417831D0 (en) * 1994-09-05 1994-10-26 Biotech & Biolog Scien Res Biological manipulation
DE19532098A1 (de) * 1995-08-30 1997-03-06 Stuemed Gmbh Vorrichtung für endoskopische Operationen, insbesondere spreizbares Stütz-Epi-Hypopharyngo-Laryngoskop nach Feyh-Kastenbauer
GB9517780D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
GB9517779D0 (en) * 1995-08-31 1995-11-01 Roslin Inst Edinburgh Biological manipulation
US5905042A (en) * 1996-04-01 1999-05-18 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Cultured inner cell mass cell lines derived from bovine or porcine embryos
US5945577A (en) * 1997-01-10 1999-08-31 University Of Massachusetts As Represented By Its Amherst Campus Cloning using donor nuclei from proliferating somatic cells

Cited By (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999005266A3 (en) * 1997-07-26 1999-04-15 Wisconsin Alumni Res Found Trans-species nuclear transfer
EP1045635A4 (en) * 1998-01-08 2001-10-24 Univ Massachusetts CLONING USING DIFFERENTIATED FETAL CELLS AND ADULT CELLS
WO1999036510A1 (de) * 1998-01-16 1999-07-22 Agrobiogen Gmbh Effizienter kerntransfer mit fetalen fibroblasten
US6600087B1 (en) 1998-01-16 2003-07-29 Agrobiogen Gmbh Efficient nuclear transfer using fetal fibroblasts and multiple cloning procedures
US6331659B1 (en) 1998-01-21 2001-12-18 University Of Hawaii Cumulus cells as nuclear donors
WO1999037143A3 (en) * 1998-01-21 2000-02-03 Univ Hawaii Full term development of animals from enucleated oocytes reconstituted with adult somatic cell nuclei
WO1999046982A1 (en) * 1998-03-16 1999-09-23 Relag Pty Ltd Porcine nuclear transfer
WO1999053025A1 (en) * 1998-04-15 1999-10-21 Monash University Method of nuclear transfer
WO1999053751A1 (en) * 1998-04-20 1999-10-28 Consorzio Incremento Zootecnico S.R.L. Source of nuclei for nuclear transfer
WO1999063061A1 (en) * 1998-06-02 1999-12-09 Biotechnology And Biological Sciences Research Council Es cell lines from somatic cells via embryo reconstruction and selective ablation
WO2000008924A1 (en) * 1998-08-11 2000-02-24 Perry Anthony C F Method of performing transgenesis
WO2000012682A1 (en) * 1998-09-01 2000-03-09 Wisconsin Alumni Research Foundation Primate embryonic stem cells with compatible histocompatibility genes
EP1127112B1 (en) * 1998-11-02 2007-03-21 Trustees Of Tufts College Methods for cloning animals
WO2000051424A3 (en) * 1999-03-04 2001-03-22 Ppl Therapeutics Scotland Ltd Genetic modification of somatic cells and uses thereof
EP2199392A2 (en) 1999-03-04 2010-06-23 Revivicor, Inc. Genetic modification of somatic cells and uses thereof
JP2011188873A (ja) * 1999-03-04 2011-09-29 Revivicor Inc 体細胞の遺伝的改変とそれらの使用
JP2002537785A (ja) * 1999-03-04 2002-11-12 ピーピーエル セラピューティクス （スコットランド） リミテッド 体細胞の遺伝的改変とそれらの使用
EP2199392A3 (en) * 1999-03-04 2010-12-15 Revivicor, Inc. Genetic modification of somatic cells and uses thereof
AU753209B2 (en) * 1999-06-30 2002-10-10 Seoul National University Industry Foundation Method for producing cloned tigers by employing inter-species nuclear transplantation technique
AU753207B2 (en) * 1999-06-30 2002-10-10 Seoul National University Industry Foundation Method for producing cloned cows
JP2003512052A (ja) * 1999-10-15 2003-04-02 アドバンスド セル テクノロジー、インコーポレイテッド 分化前駆細胞及び系統欠失胚性幹細胞の産生方法
US8753884B2 (en) 1999-10-15 2014-06-17 Advanced Cell Technology Method of differentiation of morula or inner cell mass cells and method of making lineage-defective embryonic stem cells
US20140248700A1 (en) * 1999-10-15 2014-09-04 Advanced Cell Technolgy Method of Differentiation of Morula or Inner Cell Mass Cells and Method of Making Lineage-Defective Embryonic Stem Cells
JP2003512848A (ja) * 1999-11-02 2003-04-08 ユニバーシティ オブ マサチューセッツ、アズ リプリゼンティッド バイ イッツ アマースト キャンパス 遺伝的診断、修飾および増殖のための一倍体ゲノムの使用
US7491867B2 (en) 1999-11-19 2009-02-17 Kyowa Hakko Kirin Co., Ltd. Expression of xenogenous (human) immunoglobulins in cloned, transgenic ungulates
US7414170B2 (en) 1999-11-19 2008-08-19 Kirin Beer Kabushiki Kaisha Transgenic bovines capable of human antibody production
WO2001035734A1 (en) * 1999-11-19 2001-05-25 Korea Research Institute Of Bioscience And Biotechnolgoy An improved method for production of porcine clone embryos via somatic cell nuclear transfer
EP1762624A3 (en) * 2000-01-22 2007-11-07 The University Court Of The University Of Edinburgh Methods for amplifying genetic material and uses thereof
WO2001053473A3 (en) * 2000-01-22 2002-05-30 Univ Edinburgh Methods for amplifying genetic material and uses thereof
US6906238B2 (en) 2000-03-15 2005-06-14 University Of Georgia Research Foundation, Inc. Effective nuclear reprogramming in mammals using CDK2 inhibitors
WO2001068831A3 (en) * 2000-03-15 2003-08-28 Univ Georgia Res Found Effective nuclear reprogramming in mammals
WO2002009507A1 (de) * 2000-07-27 2002-02-07 Apogene Gmbh & Co. Kg Somatischer klonierungs-gentransfer zur produktion von rekombinanten proteinen, zellen und organen
US8030537B1 (en) 2000-07-27 2011-10-04 Apogene Gmbh & Co. Kg Somatic cloning gene transfer for the production of recombinant proteins, cells and organs
US7129084B2 (en) 2000-08-03 2006-10-31 Therapeutic Human Polyclonals, Inc. Production of humanized antibodies in transgenic animals
EP1692936A1 (en) 2000-10-03 2006-08-23 GIE-CERBM, Centre Europeen de Recherche en Biologie et en Médecine Method for targeted conditional DNA recombination in mice using the cre-ert2 fusion protein
EP2138583A1 (en) 2000-12-22 2009-12-30 Institut National De La Recherche Agronomique Position-independent and tissue specific expression of a transgene in milk of transgenic animals
US7309811B2 (en) 2001-02-23 2007-12-18 Elan Pharmaceuticals, Inc Transgenic mice knockouts of BACE-1
US6489536B2 (en) 2001-03-07 2002-12-03 Director General Of National Agricultural Research Organization Method for producing cloned bovines from cells of a bovine intramuscular preadipocyte cell line
EP1238584A1 (en) * 2001-03-07 2002-09-11 Director General of National Agricultural Research Organization Method for producing livestock individuals from cells of established cell line
US7249569B2 (en) 2001-08-13 2007-07-31 Embrex, Inc. Methods for injecting avian eggs
WO2003085105A1 (en) * 2002-04-01 2003-10-16 Gtc Biotherapeutics, Inc. A method for selecting cell lines to be used for nuclear transfer in mammalian species
JP2005528095A (ja) * 2002-04-01 2005-09-22 ジーティーシー バイオセラピューティックス インコーポレイテッド 哺乳類種における核移植に使用するための細胞株を選択する方法
US7429690B2 (en) 2002-11-08 2008-09-30 Kirin Holdings Kabushiki Kaisha Transgenic bovines having reduced prion protein production
US7807863B2 (en) 2002-11-08 2010-10-05 Kyowa Hakko Kirin Co., Ltd. Transgenic bovine having reduced prion protein activity and uses thereof
US7420099B2 (en) 2004-04-22 2008-09-02 Kirin Holdings Kabushiki Kaisha Transgenic animals and uses thereof
US7928285B2 (en) 2004-04-22 2011-04-19 Kyowa Hakko Kirin Co., Ltd. Method of producing xenogenous antibodies using a bovine
US10501723B2 (en) 2005-08-03 2019-12-10 Astellas Institute For Regenerative Medicine Methods of reprogramming animal somatic cells
EP3138403A1 (en) 2005-08-09 2017-03-08 Revivicor, Inc. Transgenic ungulates expressing ctla4-ig and uses thereof
US8796021B2 (en) 2007-02-23 2014-08-05 Advanced Cell Technology, Inc. Blastomere culture to produce mammalian embryonic stem cells
US10584313B2 (en) 2007-02-23 2020-03-10 Astellas Institute For Regenerative Medicine Method of producing a differentiated mammalian cell comprising culturing a single mammalian blastomere
EP2987498A1 (en) 2008-05-02 2016-02-24 Laboratoire Français du Fractionnement et des Biotechnologies Treatment of bleeding with low half-life fibrinogen
EP3225252A1 (en) 2008-05-02 2017-10-04 Laboratoire Français du Fractionnement et des Biotechnologies Treatment of bleeding with low half-life fibrinogen
EP2267153A1 (en) 2009-05-26 2010-12-29 Université Claude Bernard Lyon 1 Identification of netrin-1 receptor unc5c gene mutation in solid cancers
WO2012020077A1 (en) 2010-08-11 2012-02-16 Inserm (Institut Mational De La Santé Et De La Recherche Médicale) Methods of growing an embryo to a blastocyst stage of development
WO2012112586A1 (en) 2011-02-14 2012-08-23 Revivicor, Inc. Genetically modified pigs for xenotransplantation of vascularized xenografts and derivatives thereof
US11179496B2 (en) 2011-02-14 2021-11-23 Revivicor, Inc. Genetically modified pigs for xenotransplantation of vascularized xenografts and derivatives thereof
US12569592B2 (en) 2011-02-14 2026-03-10 Revivicor, Inc. Genetically modified pigs for xenotransplantation of vascularized xenografts and derivatives thereof
US10865383B2 (en) 2011-07-12 2020-12-15 Lineage Cell Therapeutics, Inc. Methods and formulations for orthopedic cell therapy
CN104488853A (zh) * 2015-01-12 2015-04-08 中国农业大学 一种牛胚胎玻璃化冷冻甩管解冻和直接移植方法
WO2022109316A1 (en) 2020-11-20 2022-05-27 Revivicor, Inc. Multi-transgenic pigs with growth hormone receptor knockout for xenotransplantation
WO2023044100A1 (en) 2021-09-20 2023-03-23 Revivicor, Inc. Multitran scenic pigs comprising ten genetic modifications for xenotransplantation

Also Published As

Publication number Publication date
ES2284197T3 (es) 2007-11-01
CN1248288B (zh) 2011-11-09
WO1998030683A3 (en) 1998-09-17
IL130829A0 (en) 2001-01-28
CA2277192C (en) 2011-03-15
US20020010949A1 (en) 2002-01-24
NZ336612A (en) 2002-09-27
US5945577A (en) 1999-08-31
DE69837270T2 (de) 2008-01-24
DE69837270D1 (de) 2007-04-19
JP2007167078A (ja) 2007-07-05
ATE356203T1 (de) 2007-03-15
AU742363B2 (en) 2002-01-03
EP1808484A1 (en) 2007-07-18
AU6014598A (en) 1998-08-03
BR9806872A (pt) 2000-04-18
AU742363C (en) 2007-06-28
US20050108785A1 (en) 2005-05-19
CA2277192A1 (en) 1998-07-16
JP2001512964A (ja) 2001-08-28
EP1015572A2 (en) 2000-07-05
US6235970B1 (en) 2001-05-22
CN1248288A (zh) 2000-03-22
IL130829A (en) 2008-11-26
EP1015572B1 (en) 2007-03-07

Similar Documents

Publication Publication Date Title
AU742363C (en) Nuclear transfer with differentiated fetal and adult donor cells
US6235969B1 (en) Cloning pigs using donor nuclei from non-quiescent differentiated cells
AU740709B2 (en) Embryonic or stem-like cell lines produced by cross species nuclear transplanta tion
US6215041B1 (en) Cloning using donor nuclei from a non-quiesecent somatic cells
NZ502129A (en) Cloning using donor nuclei from non-serum starved, differentiated cells
US20010012513A1 (en) Embryonic or stem-like cell lines produced by cross species nuclear transplantation
EP1241931A1 (en) Cloning pigs using donor cells or nuclei from differentiated cells and production of pluripotent porcine.
US20050095704A1 (en) Embryonic or stem-like cell lines produced by cross species nuclear transplantation
AU2011202964A1 (en) Nuclear transfer with differentiated fetal and adult donor cells
AU2006202185A1 (en) Cloning using donor nuclei from non-serum starved, differentiated cells
AU2006236032A1 (en) Nuclear transfer with differentiated fetal and adult donor cells
MXPA00000201A (en) Cloning using donor nuclei from non-serum starved, differentiated cells
MXPA99006464A (en) Nuclear transfer with differentiated fetal and adult donor cells

Legal Events

Date Code Title Description
WWE Wipo information: entry into national phase

Ref document number: 130829

Country of ref document: IL

Ref document number: 98802794.1

Country of ref document: CN

AK Designated states

Kind code of ref document: A2

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW SD SZ UG ZW AT BE CH DE DK ES FI FR GB GR IE IT LU MC

AK Designated states

Kind code of ref document: A3

Designated state(s): AL AM AT AU AZ BA BB BG BR BY CA CH CN CU CZ DE DK EE ES FI GB GE GH HU ID IL IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MD MG MK MN MW MX NO NZ PL PT RO RU SD SE SG SI SK SL TJ TM TR TT UA UG UZ VN YU ZW AM AZ BY KG KZ MD RU TJ TM

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): GH GM KE LS MW SD SZ UG ZW AT BE CH DE DK ES FI FR GB GR IE IT LU MC

DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 336612

Country of ref document: NZ

ENP Entry into the national phase

Ref document number: 2277192

Country of ref document: CA

Ref document number: 2277192

Country of ref document: CA

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: PA/a/1999/006464

Country of ref document: MX

Ref document number: 60145/98

Country of ref document: AU

ENP Entry into the national phase

Ref document number: 1998 530958

Country of ref document: JP

Kind code of ref document: A

WWE Wipo information: entry into national phase

Ref document number: 1998903349

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642

WWP Wipo information: published in national office

Ref document number: 1998903349

Country of ref document: EP

WWG Wipo information: grant in national office

Ref document number: 1998903349

Country of ref document: EP

WWG Wipo information: grant in national office

Ref document number: 60145/98

Country of ref document: AU