Classifications

• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
  • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
  • G01N35/1081—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane
  • G01N35/109—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices characterised by the means for relatively moving the transfer device and the containers in an horizontal plane with two horizontal degrees of freedom
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N11/00—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties
  • G01N11/02—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties by measuring flow of the material
  • G01N11/04—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties by measuring flow of the material through a restricted passage, e.g. tube, aperture
  • G01N11/08—Investigating flow properties of materials, e.g. viscosity, plasticity; Analysing materials by determining flow properties by measuring flow of the material through a restricted passage, e.g. tube, aperture by measuring pressure required to produce a known flow
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
  • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
  • G01N33/483—Physical analysis of biological material
  • G01N33/487—Physical analysis of biological material of liquid biological material
  • G01N33/49—Blood
• • G—PHYSICS
  • G01—MEASURING; TESTING
  • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
  • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
  • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
  • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
  • G01N2035/1025—Fluid level sensing

Definitions

• the present invention relates to a liquid viscosity measuring device, and more particularly, to a liquid viscosity measuring device that can simultaneously measure the viscosity (viscosity) of a liquid when a liquid sample is suctioned by a dispensing device or the like.
• a collected blood sample 1 ⁇ is put into a test tube 12 and first centrifuged, whereby blood 0 is It is roughly separated into plasma component 14 and red blood cell component 16.
• a small amount of white blood cell component 18 is expressed between plasma component 14 and red blood cell component 16.
• the method of dispensing blood performed by the conventional dispensing apparatus is roughly divided into two steps, a plasma dispensing step and a red blood cell dispensing step.
• the plasma dispensing step the plasma component 14 is sucked by the nozzle tip 20 and the blood component is dispensed into a plurality of other containers 22 one by one.
• the red blood cell dispensing process the red blood cell component was aspirated by the nozzle tip 20 at a force of 16 f, then was once transferred to a diluting container (not shown), mixed with the diluent, and then diluted.
• the red blood cell component is again aspirated by the nozzle tip, and is dispensed into a plurality of other containers 24 in increments of 5.
• the reagents (for plasma component! «And red blood cell component reagents) are applied to the devices 22 and 24, respectively.
• the containers 22 and 24 are sent to the measuring device, and the coagulation measurement of the separation inside the container is performed optically. Judgment such as h type is performed.
• the sex of the red blood-requiring component is useful information in a diagnosis or the like, and is useful information as a judgment material in setting a suction pressure when dispensing blood.
• a water measuring device is not provided, and the viscosity of a blood sample or the like is measured by another measuring device.
• an object of the present invention is to provide a liquid viscosity measuring device capable of measuring viscosity simultaneously with suctioning a liquid sample.
• Another object of the present invention is to provide a dispensing device capable of measuring viscosity simultaneously with dispensing without preparing blood for viscosity measurement.
• the present invention relates to a method of obtaining the time from the start of suction of a liquid at a predetermined initial suction pressure until the pressure of the suction system reaches a predetermined value, and the viscosity of the liquid fiber.
• the present invention provides a liquid sample suction device, comprising: a nozzle tip for sucking 1 liter of liquid; a suction pump connected to the nozzle tip; and a pressure sensor for measuring a pressure of a suction system. It is assumed that a viscosity measuring device force 5 'is provided, and the viscosity of the liquid I is measured simultaneously with the suction.
• a viscosity table expressing the relationship between the arrival time and the viscosity of the liquid is created in advance, and the viscosity measuring unit measures the arrival time of the liquid, and refers to the viscous table to check the liquid I »Find the viscosity of.
• blood viscosity can be measured simultaneously with dispensing.
• Another advantage is that viscosity measurement can be performed without preparing a specific amount of blood for viscosity measurement.
• FIG. 5 shows a schematic constitutional force s of a general suction device in a dispensing device or the like.
• the nozzle tip 2 0, is the pump 1 0 4 force 5 'connected through an air hose 1 0 2.
• This pump 104 is composed of a cylinder I 06 and a piston 108.
• a pressure sensor 110 is provided to detect the internal pressure of the air hose 102.
• FIG. 6 shows a pressure change in the suction system detected by the pressure sensor 110 after the initial suction pressure is generated by pulling the piston I08 shown in FIG.
• the time from one-force to atmospheric pressure increases. Therefore, if we now consider the E force value, the time to reach / 3 (arrival time) will differ depending on the viscosity.
• FIG. 7 shows the relationship between the arrival time and the viscosity, and as shown in the drawing, the relationship is almost proportional. This is also demonstrated by calculations.
• FIG. 1 shows ⁇ If of the dispensing device 30 according to the present effort, and FIG. 1 is a perspective view thereof. [0 0 2 2]
• This dispensing device 30 is for dispensing the plasma component and the red blood cell component after centrifugation in the present embodiment, in other words, for performing preprocessing dispensing for blood type determination. .
• a nozzle part 32 for aspirating blood which is illustrated substantially at the center of the figure, is held by an XYZ robot 34, and the nozzle part 32 is freely movable three-dimensionally. I have.
• FIG. 2 shows a cross-sectional view of a main part of the nozzle section 32.
• the nozzle section 32 is composed of a nozzle base 35 and a disposable chip (hereinafter, referred to as a chip) 36 forming a nozzle tip.
• a disposable nozzle tip is used.
• the tip of the nozzle base 35 is press-inserted into the upper opening of the tip 36, and the tip of the nozzle base 35 is fitted into the upper opening of the chip 36 in this manner. Thereby, the tip 36 is securely fixed to the nozzle base 35.
• a disposable chip hereinafter, referred to as a chip
• the chip 36 is made of, for example, a plastic material, and the nozzle base 35 is made of metal. Etc.
• the X Upsilon Zeta robot 3 4 4 and X X driver 3, and Y ⁇ 3 4 y is composed of a Z drive portion 3 4 Z, the Z driving unit 3 4 z nozzle unit Elevator section with 3 2 3 8 Elevated up and down.
• the elevator section 38 has a limit switch 40 for performing functions such as a jamming sensor.
• Reference numeral 40 denotes an upwardly applied external acting force applied to the nozzle 32.
• a diluting liquid pipe 42 for discharging the diluting liquid is fixedly disposed in the Z driving section 34z.
• One end of an air hose 44 is connected to the nozzle section 32, and the other end of the air hose 44 is connected to a cylinder 46 that functions as a suction / discharge pump.
• one end of the diluent hose 48 is connected to the diluent pipe 42, and the other end is an electromagnetic valve. It is connected to a cylinder 52 via a lube 50.
• a pressure sensor 547 for measuring the internal pressure in the air hose 44 is connected between the cylinder 4-6 and the nozzle ⁇ B32.
• the signal from the limit switch 40 is sent to the apparatus main body via the signal cable 56.
• the rack 60 placed on the dispensing 58 holds a plurality of ⁇ f 62 containing the blood after the centrifugal separation force s is applied. That is, as shown in FIG. 4, the blood 62 which is separated from the plasma component and the erythrocyte component and the force s is contained in the tube 62. Further, on a horizontal table 64 provided on the dispensing table 58, a dilution tray 68 having a plurality of dilution vessels 66 and a microplate 70 are placed.
• the microplate 70 is formed with a plurality of vessels which are containers for dispensing blood components to be dispensed or diluted red blood cell components. After dispensing all blood, the microplate 70 is used as an apparatus for blood type determination, where optical determination is performed. Note that the revenge determination may be made by visual judgment.
• the nozzle tip is disposable, that is, a disposable type, a plurality of new tip forces s ' are prepared in the tip stand 72, and the tips are made in order.
• a chip disposal tray 74 is also provided.
• the blood component or the red blood cell component can be freely absorbed by the tip 36 of the nozzle portion 32 and transferred to another container.
• this dispensing device can be used for purposes other than dispensing blood ⁇ ", and various applications are possible.
• FIG. 3 is a block diagram showing a schematic configuration of the dispensing apparatus of the present embodiment.
• the internal pressure of the air hose 44 is detected by the pressure sensor 54, and the sensor signal is amplified by the DC amplifier 78.
• the amplified signal is sent to the A / D converter 82 via the limiter circuit 80.
• the limiter circuit 80 is a protection circuit for suppressing an excessive input.
• the / 0 converter 82 converts the sensor signal into a digital signal and sends it to the controller 84.
• the control section 84 is constituted by, for example, a computer or the like, and controls the internal volume of the cylinder 46, controls the XYZ robot 34, and the like.
• the control section 84 includes a viscosity measuring section 86 and a table 88. The viscometer 86 and table 88 will be described below.
• the relationship between the arrival time and the viscosity is stored in Table 88.
• a predetermined initial suction pressure I is generated by the pump under ⁇ 1 of the control unit 84, and thereafter, A pressure change has been detected by the pressure sensor 54.
• the viscosity measuring unit 86 measures the time from when the initial suction pressure is applied to when the pressure reaches a predetermined pressure value / ?. Further, the viscosity measuring unit 86 obtains the viscosity of the blood by making the measurement time correspond to the table 88. The obtained viscosity is displayed in a ⁇ part (not shown), and the result is provided to the control unit 84 for controlling the pump.
• the viscosity measuring unit 86 the viscosity can be measured simultaneously with the suction of the blood, so that no special time is required for the viscosity measurement, and the viscosity can be measured extremely easily.
• g _ PTC / JP92 / 01343 93/08475 Another advantage is that no special blood sample needs to be prepared for viscosity measurement.
• the viscosity can be easily measured by measuring the arrival time of the liquid. Therefore, there is an advantage 5 that the viscosity can be measured simultaneously with the suction of the liquid. Therefore, it is particularly effective in a dispensing device for dispensing blood ⁇ ".
• FIG. 4 is a cross-sectional view illustrating a cross section of a main part of a nozzle portion 32.
• FIG. 9 is an explanatory diagram showing the dispensing of plasma ⁇ red blood cells as preprocessing for blood type determination o
• FIG. 1 is a schematic diagram showing a schematic configuration of a general bowing device.
• FIG. 9 is an s diagram showing the relationship between the time elapsed after the initial suction pressure was applied and the change in the pressure of the suction I system for each viscosity liquid.
• FIG. 4 is an explanatory diagram illustrating a relationship between an arrival time and an angle.

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• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Physics & Mathematics (AREA)
• Chemical & Material Sciences (AREA)
• Engineering & Computer Science (AREA)
• General Health & Medical Sciences (AREA)
• Biomedical Technology (AREA)
• General Physics & Mathematics (AREA)
• Immunology (AREA)
• Pathology (AREA)
• Biochemistry (AREA)
• Analytical Chemistry (AREA)
• Hematology (AREA)
• Ecology (AREA)
• Biophysics (AREA)
• Molecular Biology (AREA)
• Urology & Nephrology (AREA)
• Food Science & Technology (AREA)
• Medicinal Chemistry (AREA)
• Automatic Analysis And Handling Materials Therefor (AREA)
• Sampling And Sample Adjustment (AREA)
• Investigating Or Analysing Biological Materials (AREA)

Priority Applications (2)

Applications Claiming Priority (2)

Publications (1)

Family

ID=17500693

Family Applications (1)

Country Status (7)

Cited By (3)

Families Citing this family (14)

Citations (11)

Family Cites Families (5)

• 1991
  • 1991-10-18 JP JP3271484A patent/JP2552408B2/ja not_active Expired - Fee Related
• 1992
  • 1992-10-12 TW TW81108084A patent/TW221672B/zh active
  • 1992-10-15 DE DE69220500T patent/DE69220500T2/de not_active Expired - Lifetime
  • 1992-10-15 AU AU27716/92A patent/AU2771692A/en not_active Abandoned
  • 1992-10-15 CA CA 2121500 patent/CA2121500A1/en not_active Abandoned
  • 1992-10-15 EP EP92921482A patent/EP0608425B1/en not_active Expired - Lifetime
  • 1992-10-15 WO PCT/JP1992/001343 patent/WO1993008475A1/ja active IP Right Grant

Patent Citations (11)

Non-Patent Citations (1)

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