WO1987000548A1 - Systeme et dispositif de fermentation dans des conteneurs souples - Google Patents

Systeme et dispositif de fermentation dans des conteneurs souples Download PDF

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Publication number
WO1987000548A1
WO1987000548A1 PCT/SE1986/000342 SE8600342W WO8700548A1 WO 1987000548 A1 WO1987000548 A1 WO 1987000548A1 SE 8600342 W SE8600342 W SE 8600342W WO 8700548 A1 WO8700548 A1 WO 8700548A1
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WO
WIPO (PCT)
Prior art keywords
soft
liquid
envelope
valve
gas
Prior art date
Application number
PCT/SE1986/000342
Other languages
English (en)
Inventor
Paul Henri LEFÈBVRE
Edouard Colinet
Henri J. L. LEFÈBVRE
Original Assignee
Alfa-Laval Food Engineering Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alfa-Laval Food Engineering Ab filed Critical Alfa-Laval Food Engineering Ab
Publication of WO1987000548A1 publication Critical patent/WO1987000548A1/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel

Definitions

  • the present invention relates to a system for fermenta ⁇ tion of initially sterile liquids by microorganisms in pure culture under conditons avoiding all infection of other microorganisms, the fermentation being either conti- nuous or not, the method using one or several soft contai ⁇ ners arranged in series or parallel to each other and being initially sterile or completely sterilized, as well as cultivation of microorganisms.
  • the invention also relates to refermentation of liquids not being recontaminated by undesired microorganisms as well as cultivation of microorganisms.
  • the soft container(s) consist of soft, flexible envelo ⁇ pes of a any general form, but especially cylindrical ones and of containers with se ispherical ends or other end sections submersed in a liquid possibly being water in which possibly are solved or dispersed solids, liquids or salts to provide the liquid with the desired features.
  • the whole system is sterilized before it is used and, if no accident occurs, the sterilization does not need to be redone during the lifetime of the system under the con ⁇ dition that the liquid to be fermented as well as the air or the oxygen are perfectly sterilized by methods known in the art before they are admitted into the system, that the culture is rigorously pure and asepticly admitted into the system, that the withdrawal of fermented and stored liqu ⁇ ids is aseptically performed and that the content in the soft container is kept in circulation, this being achieved by pumping or by a mixer, and also that the evacuation of the fermentation gases does not allow any microorganism others than the ones being used to enter the liquid, this being the object of a controlled fermentation of a selec ⁇ ted microorganism.
  • the soft container at the start being empty and steri ⁇ le is aseptically fed with sterile liquid and with pure culture and oxygen or sterile air according to the requi ⁇ rements considering the type of microorganism and thus the fermentation being used, the sterile liquid having a low content of gas.
  • the liquid is mixed e.g. by pumping in a closed circuit. It is kept at the desired temperature and is completely protected against parasitic fermentation, the possibly occuring fermentation gases being evacuated from the upper part of the soft container to the exteriors or to a recuperating device passing through an apparatus eliminating all hazards of reinfection of the content in the soft container.
  • the microorganisms are kept dispersed in the soft con ⁇ tainer, the content of which is kept in circulation, the deposits with a significant thickness on the containers soft diaphragm being regularly separated due to the exte ⁇ rior action on these soft diaphragms by localized forces causing deformations of the diaphragm; these localized forces are provided mechanically or hydraulically by loca ⁇ lized beams using the liquid in which the soft container is immersed.
  • the circulating pump guaranteeing the circulation of the liquid during the process of fermentation is of a type with large capacity and low pressure or of a type with low capacity and high pressure, if the circulation of the con ⁇ tent in the soft container is maintained indirectly by a hydroejector, but this pump is preferably an aseptic pump of a new type to be described below (figure 2) or an ana ⁇ logous pump.
  • This fluid is chosen amongst a variety of possible com ⁇ positions so that it cannot damage the organoleptic pro ⁇ perties of the liquid transported by the pump or the health of the user if traces thereof are penetrating into the liquid.
  • the aseptic pump described in figure 2 is badly suited for the purpose, if the quantity per hour is very large, of the order of 500-1000 m or more.
  • the diameter of the pipes are lying between 0,5 and 1 m and even more and, moreover, it is preferred to design the pump in accordance with the figures of the drawing.
  • the movable container 7 is not conve ⁇ nient. It is replaced by a chamber or a tank with large dimensions.
  • the pump or the pipe including a screw, its shaft and the packing of the pump and also the flanges for connec ⁇ tion to the net of rigid piping are sterilized.
  • the antiseptic liquid is then directly poured over the normally very warm and sterile parts, through which a re- infection could be possible if there is a microleakage, whereafter the tank is quickly filled with antiseptic li ⁇ quid, thus being equivalent to the container 7 in figure 2
  • Tight valves with very large diameters are not necessa ⁇ ry when, in case of a repair, it is important to isolate the very large pump of the soft containers , because, in case the pressure In the liquid piping does not surmount 0,5 kg/cm2, it is sufficient to flatten two soft sections of the piping for transmission of the liquid, one down ⁇ stream and the other upstream of the circulating pump, to be able to isolate the latter one. It then remains in a proper way to resterilize the part of the device which has been Infected and to reinstall the circulating pump for operation guaranteeing the total and permanent aseptic condition due to the antiseptic liquid.
  • the exterior pressure on the soft envelopes can be maintained hydraulically for obtaining pressure balance between the inner and the outer parts of the enve ⁇ lope.
  • the pump includes a steam inlet, an outlet for condensate and a device for feeding in sterile air or inert gas.
  • the simplest antiseptic liquids suitable are concentra ⁇ ted solutions of caustic soda or potassium hydroxide as well as strong acids such as sulphuric acid, but some ten other compositions can also achieve the aseptic condition in simpler apparatus which not have been especially desig- nad for aseptic operation with one or several possibili ⁇ ties for microleakage which allow for reinfection of the product circulating in the apparatus.
  • the latter can be a pump, a dispersing device, a colloidal grinding device, a continuous centrifugal separator and even a simple connec ⁇ tion consisting of dis ountable flanges or pipe fittings.
  • a remarkable property of this new technology is that the preservation of the aseptic condition is guaranteed during extremely long periods of time in a very simple, reliable and cheap manner, the elimination of hazards for reinfection being total as long as, from time to time, a survey is made of the volume of the bactericidal immersion liquid and its composition.
  • the diaphragm forming the soft containers wall can be chosen as a function of what the liquid requires and the fermentation it is submitted to.
  • the method of the present invention is using one or seve ⁇ ral of the methods below:
  • the process allows the performing of discontinous fer ⁇ mentations, the container being filled, the liquid being fermented and the fermented liquid then been transferred to other containers, equal or unequal. This way of opera- tion is necessary for certain fermentations.
  • the fermented beer in cir ⁇ culation having a density of the order of 1,015 with an initial, already augmented density of 1,072 by way of ex- ample.
  • diaphragm and of the treatment for dega- sing the immersion liquid depends on the grade of anaero- bicity to be preserved in the liquid during fermentation.
  • the thickness of the barrier layer can be augmented or the content of oxygen in the immersion liquid be reduced.
  • the immersion liquid is degassed and is covered with a barrier film or a gas very poor of oxygen or these precau ⁇ tions are accumulated.
  • 300m container about 2,13 cm oxygen/day. The oxidation is zero.
  • the rigid containers from be ⁇ neath with deaerated and/or cooled water.
  • the soft buffert containers with the only task to asep- ticly store sterile fermentable products can in may cases be sterilic-d before they are put into place.
  • the same is valid for certain soft containers, pipings and apparatus being used within the scope of the present invention, whereas other devices preferably are designed to be steri- lized before they are put into place and to be able to be resterilized each time it is appropiate to use e.g. a new pure culture of the microorganism in question or even another type of microorganism.
  • thermoresistant spores 100 C during 20 hours to destroy most of the thermoresistant spores to increase the letha ⁇ lity of the steam or the humid heat due to one or several classical chemical products such as strong acids, alkali, formaldehyde, hydroperoxide, halogen and derivats of chlo ⁇ rine or iodine, quaternary ammonia compounds, nitrites, sulphuric acid.
  • Germicides are added in gaseous form or in form of a mist in the steam flow or in a mixture of 'sterile warm gas and mist of warm water' , if there is a need for opera- o ting below 100 C.
  • a new technique for sterilization of a combination of pipes, soft envelope - rigid pipe or apparatus - soft en ⁇ velope consists of a sterilization of the rigid element in an autoclave and under pressure.
  • FIG. 3 shows the necessary sterilization device
  • the clamps 8 are adapted and then the rigid element 2 is ele ⁇ vated between the reinfected parts.
  • the pressure is slowly reduced and air or a neutral gas is admitted at 7 depending on the application.
  • This apparatus is especially interesting for steriliza ⁇ tion units and for aseptic cooling independent of the fluid to be treated, as It allows to eliminate the hazards for reinfection and even to maintain at an elevated tempe ⁇ rature the interior of the tight valves equipped e.g. with diaphragms.
  • The. interior of the unit in figure 5 can be sterilized independent of the soft containers and their pipes, the most common case being a simultaneous sterilization of the entire system.
  • gases steam and antiseptic agent or a mixture of sterile, non condensable gas, steam and antiseptic agent
  • the gases being used to sterilize the soft containers and their ac ⁇ cessories and pipes are entering from 1 in at 2 and steri- lize the contents in the gas holder 3, being already par ⁇ tially disinfected by the circulation of the antiseptic liquid 7 by the pump 8 and the distributor 9.
  • the liquid is heated up.
  • the ac ⁇ cessories and the pipes the warm or cooking liquid 7 is concentrated with antiseptic agent and the pump 8 is star ⁇ ted.
  • antiseptic agent is varying depending on, if the gas arriving in the gas holder is clean or polluted with organic agent.
  • the antiseptic agent being scarcely sensitive to orga- nic agents is e.g. formaldehyde, sulfltes in a slightly acid environment andhalogen derivats of acetic acid.
  • o Acidified water being kept above 70 C is also suitable, but it is preferred to reduce its pH-value to 1 by a non volatile acid such as sulphuric acid and/or adding an an- tiseptic agent as the aseptic condition must be maintained during power failures.
  • Many fermentations and productions of microorganisms require an insertion into a pilot plant, in which the soft containers have a lower capacity per unit in the order of 1 m or less.
  • the stirring of the contents in the small containers can be effected without a pump by exposing these contai ⁇ ners walls to deformations.
  • the rotating, centrifugal or volumetric pumps being made aseptic are not in all cases suitable in cases of in- dustrial plants, especially pilot plants.
  • the aseptic transfer of a sterile fermentable liquid, stored in a sterile soft container, to a fermentation con ⁇ tainer requests not only an aseptic metering pump but also that It does not allow the microorganisms to contaminate the sterile liquid upstream of the pump.
  • Aseptic extraction of the deposits of organic material or of sediments of microorganisms in the conical bottom in a static decanting device does not permit foreign microor ⁇ ganisms comtaminating the outlet into the open air to penetrate into the decanting device, one microorganism being sufficient.
  • the quantites per hour to be considered can vary extre ⁇ mely, the maximum lying in the order of 10.000 litres and the minimum at 0,0003 litres. This minimum corresponds to the sedimentary volume coming from 100 litres refermented material in one year.
  • Equal to 1 11 Equal to 2, but this is an outlet valve or pressure valve, normally closed.
  • the length of the pumps 7 body and the diameter of the envelope 16-4-20 as well as the form of the envelope bet ⁇ ween the valves 2/3 and 11/12 are functions of the desired quantity per cycle.
  • An possibly sterile cylindrical envelope can be posi ⁇ tioned after that the valves 2/3 and 11/12 have been ope ⁇ ned with the openings 5 and 13.
  • the cofferdam has been tightened with the packings 17 and 21, pressing the envelope 16-20 together against the semi-rigid pipes 15-19, supported by the rigid walls in the pipes 14-18.
  • the semi-rigid pipes 15-19 serve as seals and also to support the soft envelope at the end of the rigid pipes 14-18 at the side of the cofferdam. They prevent the soft envelope to deform In a dangerous way.
  • the cofferdam When the cofferdam is tight and the interior of the soft envelope is sterile or sterilized, the cofferdam is filled completely through the pipe 8 with cold water or other liquid with low boiling point.
  • the valve 9 is closed.
  • the pump is ready to operate as follows:
  • a vacuum at 8 acts on the soft envelope at 4 and on the immersed motor of the activating device 2, withdrawing from the stationary support 3 « The pump sucks up the li ⁇ quid at 22.
  • the vacuum is abolished at 8.
  • the activating device for the suction 2 is closed.
  • the activating device 11 is opened, the pressure rising around the envelope which is flattened and discharges its content at 23.
  • valves 2/3 and 11/12 include one or several clamps compressing the envelope against the stationary supports 3 and 12 and, if necessary to avoid the presence of microor ⁇ ganisms downstream of the nonreturn valve situated in the interior of the cofferdam, at least one valve c the type shown in figure 7 must be installed, downstream of the discharge seal 21.
  • Figure 7 shows:
  • This valve supplied with a spring, is of a type nor ⁇ mally closed.
  • the liquid filling the pipe 8 and the cof ⁇ ferdam 1-7-10 in figure 6 are entering the hydraulic motor acting on the driving device 26 and the valve in figure 7 opens even when the pressure in the cofferdam is dischar ⁇ ging the products in the envelope of the cofferdam through the valve 11/12.
  • the compression of the envelope between 25 and 27 quickly discharges the contents thereof and also the interior of the end of the envelope is not infected, considering the fequency of cleaning and the fact that the soft envelope Is completely flattened over a considerable length between two parallel surfaces, i.e. the surface of the support 27 and the surface of the plate.
  • Figure 7 is schematic and relates only to the principle of this valve, its plate possibly having another profile and evidently with a decreasing thickness at the hinge 24 at the end where the driving device 26 is acting.
  • the security is increased by placing more than one val ⁇ ve of the type shown in figure ⁇ 7 in series, downstream of the pump, and one can even exchange the nonreturn valve 11/12 in it with a valve of the type shown in figure 1.
  • This valve can as a sealing device, instead of the spring 25, include a spring with double driving devices, being one the driving device 26 and the other a driving device parallel with the first one acting on the other end of the plate.
  • Another device comprises a compressor for the soft, un ⁇ der pressure inflated envelope, which normally flattens the envelope but rises when this discharge valve has to be opened.
  • the valv(s) of the type shown in figure 7 can be re ⁇ placed by a socket valve or a diaphragm, normally closed, which is opened and closed at the same time as the valve 11/12 in figure 6.
  • the ideal is to have at least two final valves in series and that the compression of the envelope or the diaphragm is less in the final valve.
  • the cofferdam 1 is In connection with the source for vacuum and the cofferdam 10 with the source for pressure.
  • vacuum and pressure been produced e.g. by a piston pump the suction side of which is connected to the inlet cofferdam and the pressure side with the dis ⁇ charge cofferdam.
  • Pressure variations in the central cof ⁇ ferdam can be obtained thanks to interior and exterior pipes each of them comprising a valve for quantity control and even a spring device. This allows to reduce the load on the diaphragm due to the feeding to the opening of the valves.
  • a decanting device is convenient, with soft walls initially sterile and possibly having a conduit for evacuating the gas, if it is supplied with an aseptic pump acoording to figures 6 and 7. At least the bottom of the decanting device is conical or pyramidal. Its soft walls are deformed slowly but regularly with the purpose to loosen the deposits.
  • conduits for gas are not shown in figure 10, being a side view.
  • the diameter of the conduits can also be augmented due to that these conduits permit workers and even certain ap- paratus to enter. This fact simplifies and improves the production and the installation of the units.
  • domes 8 and 9 possible domes are not shown impro ⁇ ving the collecting of gas. They are situated on the con ⁇ tainers 1 in figure 8 and on the conduits 4 in figure 9. These are details the importance of which is a function of the type of fermentation or cultivation to be performed.
  • Figure 11 represents a cut through a building compri ⁇ sing a single soft container: 1-13 : See figure 1 .
  • the wall of the soft containers with large capacity facing their discharge openings or liquid outlets is rein ⁇ forced and supplied with tension bolts or exterior cables limiting their displacement. They inhibit that the dia- phragm obstructs the opening and stops, if needed, the discharge and acts on an indi .tor for the discharge de ⁇ gree. Another safety device interrupts the filling before an excessive pressure is developed In the diaphragm.
  • At least one part of the soft containers containing the liquid is Immersed.
  • the proportion between the height of liquid and the height of gas can be any one. This depends on the type of fermentation or cultivation.
  • the part of the container filled with gas can also been kept together with any mechanical means whatsoever to par ⁇ tially stabilize the form of the soft containers and to reduce the Thus overpressure to be kept therein, this being achieved by the sterilization washing device descri ⁇ bed in figure •
  • Beer from top fermentation is significantly improved by refermentation in the bottle.
  • the beer refermented in the bottle, is expensive as the storage applies to the packaged product. It has consi ⁇ derable hazards and many comsumers and retailers do not appreciate the presence of deposits on the bottom of the bottle. Therefore only small quantities of luxurious beer are recommended.
  • the aforementioned example is using wort suitable for a top fermentation beer.
  • the sterile wort is asepticly coo ⁇ led and Is stored asepticly in immersed soft containers.
  • the sterile wort is kept in circulation in the aseptic storage container as a buffer and some part with internal haze or deposits in suspension is eliminated asepticly during its aseptic transfer to the soft container for main.
  • fermentation containing yeast for top fermentation lead in asepticly at the time of the start of the device.
  • the o fermentation between 15 and 30 C takes 3-7 days under asepticly performed mixing.
  • the continously fermented beer contains maximum 0,595-1,38% not fermented wort, and also either sterile wort or a sterile sugar sirup is lead in in an aseptic way at the time for the transfer to the soft container(s) for secondary fermentation.
  • the surplus of yeast, mainly lumps and large cells are separated from this flow of green beer by a separator being accordingly regulated.
  • _ o lasts 15-60 days at a temperature of between 15 and 30 C.
  • the mature stale beer is then treated according to well known rules common for the brewery trade by centrifugation or filtration, carbonizing and is tapped on barrels, bott ⁇ les or cans.
  • the stabilization of the beer becomes rather higher than that of top fermented, not refermented beer.
  • the packaged beer re ⁇ quires very small contents of oxygen. This is achieved with new commercial tapping devices (less than 0,1 ppm oxygen after packaging) .
  • the capa ⁇ city of the container(s) is varying for the main"fermenta ⁇ tion between 3X and 7X and that for the secondary fermen ⁇ tation unit from 15X to 60X and it thus is sufficient to adapt the capacity and/or the number of soft containers and their accessories to cover all needs from a pilot plant to a very large brewery.
  • Example 2 This example concerns low fermentation beer. The slower o main fermentation is run rather below 10 C and the secon- o dary fermentation between 1 and 5 c «
  • Continuous fermentation is once again not suitable and therefore it is performed in cylindrical-conical tanks without tryirg to achieve a carbonisation of the fermenta ⁇ tion with carbonic acid.
  • the beer can be sold but it is purified by continuous refermentation. This requires a degassing of the beer, its filtration on fuller's earth or its centrifugation and then pasteurization. The sterile and cooled stale beer is then asepticly lead into the refermentation unit. This latter and the sequence of ope ⁇ rations are analogues to those described in example 1 with the exception of the temperature.
  • an aseptic buffer container is possibly jus- tified as a function of the discharge frequency of the tanks for the main fermentation.
  • the conditions are the same as for example 1, only that one fraction of the green beer after clarifying, pasteuri ⁇ zation and cooling is subjected to to a fermentation in a soft container with the assistance of a selected lactic acid bacterium.
  • the proportions of products been treated with yeast or with a lactic acid depend on the requested beer type and even on the amount of extract which can be fermented by the lactic acid bacteria.
  • the sour beer is filtrated, pasteurized, cooled and then asepticly mixed with the 'mature* beer.
  • the mixture is subjected to a complementar " y maturing improving especially the colloidal stability.
  • the stabilized mixture is then packed as in example 1 according to the rules in this technique.
  • a pilot plant has been erected for a quick start of a fermentation process.
  • This device is using a number of soft containers, whose uniform capacity is less than lm .
  • the unit comprises a big soft container, in which the quantity of sterile and cooled liquid to be fer ⁇ mented is stored protected from all oxidization and the aseptic accessories such as decanting devices, small hyd- rcyclones, sterilizable and movable filters and continuous mini-pasteurizers.
  • Each soft container is immersed in deaerated water.
  • the water serves as a control for the temperature and of the oxidization of the products via the soft containers, pipes and accessories.
  • the parameters varying during the tests are several: the temperatures, types of microorganisms, times and quan ⁇ tities, contents of microorganisms, mixtures of products been fermented by the different microorganisms, elimina- tion of a surplus of microorganisms, decantation, centri ⁇ fugation, filtration.

Abstract

La fermentation ou la culture de micro-organismes a lieu dans des réceptacles souples qui sont stériles au commencement de la fermentation. Le réceptacle souple contient le milieu qui doit fermenter. Le réceptacle, qui possède une enveloppe flexible, est immergé dans un liquide possédant les qualités désirées, par exemple, par rapport à la densité de liquide. Le milieu dans le réceptacle peut être maintenu en agitation en exerçant une action depuis l'extérieur de l'enveloppe flexible.
PCT/SE1986/000342 1985-07-25 1986-07-24 Systeme et dispositif de fermentation dans des conteneurs souples WO1987000548A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
LU86023 1985-07-25
LU86023A LU86023A1 (fr) 1985-07-25 1985-07-25 Systeme et appareillage de fermentation en reservoirs souples

Publications (1)

Publication Number Publication Date
WO1987000548A1 true WO1987000548A1 (fr) 1987-01-29

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EP (1) EP0231333A1 (fr)
AU (1) AU6147186A (fr)
LU (1) LU86023A1 (fr)
WO (1) WO1987000548A1 (fr)

Cited By (7)

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Publication number Priority date Publication date Assignee Title
EP0258795A2 (fr) * 1986-08-27 1988-03-09 Kawasumi Laboratories, Inc. Méthode et appareil pour la culture de cellules
WO2000066706A1 (fr) * 1999-05-04 2000-11-09 Roell Marcel Bioreacteur
FR2803853A1 (fr) * 2000-01-17 2001-07-20 Farzin Sarem Dispositif de culture cellulaire et tissulaire a circulation perfectionnee de fluide de culture
EP2543719A1 (fr) 2011-07-08 2013-01-09 Zellwerk GmbH Bioréacteur Mäander et procédé d'expansion, de différenciation et de récolte dynamiques de cellules hématopoïétiques
WO2015145954A1 (fr) * 2014-03-28 2015-10-01 東洋製罐グループホールディングス株式会社 Procédé de culture cellulaire et système de culture cellulaire
JP5892216B1 (ja) * 2014-09-17 2016-03-23 東洋製罐グループホールディングス株式会社 細胞培養システムにおける送液方法、及び細胞培養システム
NO20172006A1 (no) * 2017-12-18 2018-09-03 Leif Stana Sider og vinproduksjon i flytende foliebag

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FR1215772A (fr) * 1958-11-17 1960-04-20 Installations pour fermentations biologiques liquides
GB1273495A (en) * 1970-09-22 1972-05-10 Porter Lancastrian Ltd Storage and dispensing of carbonated beverages
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DE3515615A1 (de) * 1984-04-30 1985-10-31 Hickinbotham Winemakers PTY. Ltd., Burwood, Victoria Verfahren und vorrichtung zur alkoholfermentation
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GB615258A (en) * 1943-01-12 1949-01-04 Jean Emile Lucien Leperre Methods and means for casking and drawing-off beer and like liquids in a sterilized manner and protecting said liquids from air
FR1215772A (fr) * 1958-11-17 1960-04-20 Installations pour fermentations biologiques liquides
GB1273495A (en) * 1970-09-22 1972-05-10 Porter Lancastrian Ltd Storage and dispensing of carbonated beverages
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Cited By (17)

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Publication number Priority date Publication date Assignee Title
EP0258795A2 (fr) * 1986-08-27 1988-03-09 Kawasumi Laboratories, Inc. Méthode et appareil pour la culture de cellules
EP0258795A3 (en) * 1986-08-27 1988-12-07 Kawasumi Laboratories, Inc. A method for cultivating cells and an instrument therefor
WO2000066706A1 (fr) * 1999-05-04 2000-11-09 Roell Marcel Bioreacteur
CH697035A5 (de) * 1999-05-04 2008-03-31 Marcel Roell Bioreaktor.
FR2803853A1 (fr) * 2000-01-17 2001-07-20 Farzin Sarem Dispositif de culture cellulaire et tissulaire a circulation perfectionnee de fluide de culture
EP1118657A1 (fr) * 2000-01-17 2001-07-25 Cell Tissue Progress Dispositif de culture cellulaire et tissulaire à circulation perfectionnée de fluide de culture.
WO2001053449A1 (fr) * 2000-01-17 2001-07-26 Cell Tissue Progress Dispositif de culture cellulaire et tissulaire a circulation perfectionnee de fluide de culture
US6271027B1 (en) 2000-01-17 2001-08-07 Cell Tissue Progress Cell and tissue culture device with enhanced culture fluid flow
EP2543719A1 (fr) 2011-07-08 2013-01-09 Zellwerk GmbH Bioréacteur Mäander et procédé d'expansion, de différenciation et de récolte dynamiques de cellules hématopoïétiques
DE102011106914A1 (de) 2011-07-08 2013-01-10 Zellwerk Gmbh Mäander- Bioreaktor und Verfahren zu dynamischen Expansion, Differenzierung und Ernte von hämatopoetischen Zellen
WO2015145954A1 (fr) * 2014-03-28 2015-10-01 東洋製罐グループホールディングス株式会社 Procédé de culture cellulaire et système de culture cellulaire
JP2015188392A (ja) * 2014-03-28 2015-11-02 東洋製罐グループホールディングス株式会社 細胞培養方法、及び細胞培養システム
JP5892216B1 (ja) * 2014-09-17 2016-03-23 東洋製罐グループホールディングス株式会社 細胞培養システムにおける送液方法、及び細胞培養システム
WO2016042741A1 (fr) * 2014-09-17 2016-03-24 東洋製罐グループホールディングス株式会社 Procédé de distribution de liquide pour système de culture cellulaire, et système de culture cellulaire
CN106661526A (zh) * 2014-09-17 2017-05-10 东洋制罐集团控股株式会社 细胞培养系统中的送液方法以及细胞培养系统
NO20172006A1 (no) * 2017-12-18 2018-09-03 Leif Stana Sider og vinproduksjon i flytende foliebag
NO342919B1 (no) * 2017-12-18 2018-09-03 Leif Stana Sider og vinproduksjon i flytende foliebag

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EP0231333A1 (fr) 1987-08-12
LU86023A1 (fr) 1986-08-04
AU6147186A (en) 1987-02-10

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