USRE44962E1 - Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants - Google Patents
Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants Download PDFInfo
- Publication number
- USRE44962E1 USRE44962E1 US14/017,108 US201314017108A USRE44962E US RE44962 E1 USRE44962 E1 US RE44962E1 US 201314017108 A US201314017108 A US 201314017108A US RE44962 E USRE44962 E US RE44962E
- Authority
- US
- United States
- Prior art keywords
- val
- ala
- pro
- arg
- gene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- XDTMQSROBMDMFD-UHFFFAOYSA-N C1CCCCC1 Chemical compound C1CCCCC1 XDTMQSROBMDMFD-UHFFFAOYSA-N 0.000 description 2
- AMWRNGUBJXXNQP-UHFFFAOYSA-N C(CC1)CCC11CCC2(CCCCC2)CC1 Chemical compound C(CC1)CCC11CCC2(CCCCC2)CC1 AMWRNGUBJXXNQP-UHFFFAOYSA-N 0.000 description 1
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N CP(=O)(O)CCC(N)C(=O)O Chemical compound CP(=O)(O)CCC(N)C(=O)O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 1
- GINJFDRNADDBIN-UHFFFAOYSA-N [H]C(N)(CCP(C)(=O)O)C(=O)NC([H])(C)C(=O)NC([H])(C)C(=O)O Chemical compound [H]C(N)(CCP(C)(=O)O)C(=O)NC([H])(C)C(=O)NC([H])(C)C(=O)O GINJFDRNADDBIN-UHFFFAOYSA-N 0.000 description 1
- FYOUEMXQLBSXLS-JTWCQKHYSA-N [H]P(=O)(O)CCC(N)C(=O)NC(C)C(=O)N[C@@H](C)C=O.[H]P(=O)(O)CCC(N)C(=O)O Chemical compound [H]P(=O)(O)CCC(N)C(=O)NC(C)C(=O)N[C@@H](C)C=O.[H]P(=O)(O)CCC(N)C(=O)O FYOUEMXQLBSXLS-JTWCQKHYSA-N 0.000 description 1
- UHOVQNZJYSORNB-UHFFFAOYSA-N c1ccccc1 Chemical compound c1ccccc1 UHOVQNZJYSORNB-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/12—Processes for modifying agronomic input traits, e.g. crop yield
- A01H1/122—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- A01H1/123—Processes for modifying agronomic input traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
- C12N15/8277—Phosphinotricin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S47/00—Plant husbandry
- Y10S47/01—Methods of plant-breeding and including chromosome multiplication
Definitions
- the invention relates to a process for protecting plant cells and plants against the action of glutamine synthetase inhibitors.
- It relates further to non-biologically transformed plant cells and plants displaying resistance to glutamine synthetase inhibitors as well as to suitable DNA fragments and recombinants containing nucleotide sequences encoding resistance to glutamine synthetase inhibitors.
- Glutamine synthetase (hereafter simply designated by GS) constitutes in most plants one of the essential enzymes for the development and life of plant cells. It is known that GS converts glutamate into glutamine. GS is involved in an efficient pathway (the only one known nowadays) in most plants for the detoxification of ammonia released by nitrate reduction, aminoacid degradation or photorespiration. Therefore potent inhibitors of GS are very toxic to plant cells. A particular class of herbicides has been developped, based on the toxic effect due to inhibit inhibition of GS in plants.
- These herbicides comprise as active ingredient a GS inhibitor.
- Bialaphos and phosphinothricin are two such inhibitors of the action of GS, (ref. 16, 17) and have been shown to possess excellent herbicidal properties (see more particularly ref. 2 as concerns Bialaphos).
- Bialaphos has the following formula (I)
- PPT has the following formula (II)
- Bialaphos was first disclosed as having antibiotic properties, which enabled it to be used as a pesticide or a fungicide.
- Bialaphos can be produced according to the process disclosed in U.S. Pat. No. 3,832,394, assigned to MEIJI SEIKA KAISHA LTD., which patent is incorporated herein by reference. It comprises cultivating Streptomyces hygroscopicus, such as the strain available at the American Type Culture Collection, under the ATCC number 21,705, and recovering Bialaphos from its culture medium. However, other strains, such as Streptomyces viridochromogenes, also produce this compound (ref. 1).
- tripeptide antibiotics which contain a PPT moiety are or might be discovered in nature as well, e.g. phosalacin (ref. 15).
- PPT is also obtained by chemical synthesis and is commercially distributed by the industrial Company HOECHST.
- Streptomyces species which produce highly active antibiotics which are known to incapacitate procaryotic cell functions or enzymes.
- the Streptomyces species which produce these antibiotics would themselves be destroyed if they had not a self defence mechanism against these antibiotics.
- This self defence mechanism has been found in several instances to comprise an enzyme capable of inhibiting the antibiotic effect, thus of avoiding autotoxicity for the Streptomyces species concerned. This modification is generally reversed when the molecule is exported from the cell.
- Streptomyces hygroscopicus ATCC 21,705 also possesses a gene encoding an enzyme responsible of the inactivation of the antibiotic properties of Bialaphos. Experiments carried out by the applicants have lead to the isolation of such a gene and its use in a process for controlling the action of GS inhibitors, based on PPT or derived products.
- An object of the invention is to provide a new process for controlling the action in plant cells and plants of GS inhibitors.
- Another object of the invention is to provide DNA fragments and DNA recombinants, particularly modified vectors containing said DNA fragments, which DNA fragments contain nucleotide sequences capable, when incorporated in plant cells and plants, to protect them against the action of GS inhibitors.
- a further object of the invention is to provide non-biologically transformed plant cells and plants capable of neutralizing or inactivating GS inhibitors.
- a further object of the invention is to provide a process for selectively protecting plant species against herbicides of a GS inhibitor type.
- an object of the invention is to provide a DNA fragment transferable to plant cells- and to whole plants—capable of protecting them against the herbicidal effects of Bialaphos and of structurally analogous herbicides.
- a further object of the invention is to provide plant cells resistant to the products of the class examplified by Bialaphos, which products possess the PPT unit in their structure.
- the process according to the invention for controlling the action in plant cells and plants of a GS inhibitor when contacted therewith comprises providing said plants with a heterologous DNA fragment including a foreign nucleotide sequence, capable of being expressed in the form of a protein in said plant cells and plants, under condition such as to cause said heterologus DNA fragment to be integrated stably through generations in the cells of said plants, and wherein said protein has an enzymatic activity capable of inactivating or neutralization of said glutamine synthetase inhibitor.
- a preferred DNA fragment is one derived from an antibiotic-producing-Streptomyces strain (or a sequence comprising a nucleotide sequence encoding the same activity) and which encodes resistance to a said GS inhibitors.
- Preferred nucleotide sequences for use in this invention encode a protein which has acetyl tranferase activity with respect to said GS inhibitors.
- a most preferred DNA fragment according to the invention comprises a nucleotide sequence coding for a polypeptide having a PPT acetyl transferase activity.
- a particular DNA fragment according to the invention for the subsequent transformation of plant cells, consists of a nucleotide sequence coding for at least part of a polypeptide having the following sequence (SEQ ID NO:1):
- a preferred DNA fragment consists of the following nucleotide sequence (SEQ ID NO:2):
- the invention also relates to any DNA fragment differing from the preferred one indicated hereabove by the replacement of any of its nucleotides by others, yet without modifying the genetic information of the preferred DNA sequence mentioned hereabove (normally within the meaning of the universal genetic code), and furthermore to any equivalent DNA sequence which would encode a polypeptide having the same properties, particularly a Bialaphos-resistance-activity.
- the invention pertains to DNA fragments which, when introduced into such plant cells, would also confer on them a protection against other GS inhibitors, for instance of intermediate products involved in the natural biosynthesis of phosphinotricin, such as the compounds designated by the abbreviations MP101 (III), MP102 (IV), the formula of which are indicated hereafter:
- the invention has opened the route to the production of DNA fragments which, upon proper incorporation into plant cells and plants, can protect them against GS inhibitors when contacted therewith, as this will be shown in a detailed manner in relation to Bialaphos and PPT in the examples which will follow.
- any fragment encoding an enzymatic activity which would protect plant cells and plants against said GS inhibitors, by inactivationg, should be viewed as an equivalent of the preferred fragments which have been disclosed hereabove. This would apply especially to any DNA fragments that would result from genetic screening of the genomic DNAs of strains, particularly of antibiotic-producing strains, likely to possess genes which, even-though structurally different, would encode similar activity with respect to Bialaphos or PPT, or even with respect to other GS inhibitors. This applies to any gene in other strains producing a PPT derivative.
- the invention also relates to DNA recombinants containing the above defined Bialaphos-resistance DNA fragments recombined with heterologous DNA, said heterologous DNA containing regulation elements and said Bialaphos-resistance DNA being under the control of said regulation elements in such manner as to be expressible in a foreign cellular environment compatible with said regulation elements.
- the abovesaid Bialaphos-resistance-DNA fragments contained in said DNA recombinants are devoid of any DNA region involved in the biosynthesis of Bialaphos, when said Bialaphos-resistance-DNA fragment originate themselves from Bialaphos-producing strains.
- heterologous DNA is meant a DNA of an other origin than that from which said Bialaphos-resistance-DNA originated, e.g. is different from that of a Streptomyces hygroscopicus or Streptomyces viridochromogenes or even more preferably a DNA foreign to Streptomyces DNA.
- Particularly said regulation elements are those which are capable of controlling the transcription and translation of DNA sequences normally associated with them in said foreign environment.
- Cellular refers both to microorganisms and to cell cultures.
- This heterologous DNA may be a bacterial DNA, particularly when it is desired to produce a large amount of the recombinant DNA, such as for amplification purposes.
- a preferred heterologous DNA consists of DNA of E. coli or of DNA compatible with E. coli. It may be DNA of the same origin as that of the cells concerned or other DNA, for instance viral or plasmidic DNA known as capable of replicating in the cells concerned.
- Preferred recombinant DNA contains heterologous DNA compatible with plant cells, particularly Ti-plasmid DNA.
- Particularly preferred recombinants are those which contain GS inhibitor inactivating DNA under the control of a promoter recognized by plant cells, particularly those plant cells on which inactivation of GS inhibitors is to be conferred.
- Preferred recombinants according to the invention further relate to modified vectors, particularly plasmids, containing said GS-inhibitor-inactivating DNA so positioned with respect to regulation elements, including particularly promoter elements, that they enable said GS inhibitor-inactivating DNA to be transcribed and translated in the cellular environment which is compatible with said heterologous DNA.
- Advantageous vectors are those so engineered as to cause stable incorporation of said GS inhibitor inactivating DNA in foreign cells, particularly in their genomic DNA.
- Preferred modified vectors are those which enable the stable transformation of plant cells and which confer to the corresponding cells, the capability of inactivating GS inhibitors.
- the initiation codon of the Bialaphos-resistance-gene of the Streptomyces hygroscopicus strain used herein is a GTG codon.
- the Bialaphos-resistance-gene is modified by substitution of an ATG initiation codon for the initiation codon GTG, which ATG enables translation initiation in plant cells.
- the plant promoter sequence which has been used was constituted by a promoter of the 35 S cauliflower mosaic virus. Needless to say that the man skilled in the art will be capable of selecting other plant promoters, when more appropriate in relation to the plant species concerned.
- the heterologous DNA fragment is fused to a gene or DNA fragment encoding a transit peptide, said last mentioned fragment being then intercalated between the GS inhibitor inactivating gene and the plant promoter selected.
- transit peptide refers to a polypeptide fragment which is normally associated with a chloroplast protein or a chloroplast protein sub-unit in a precursor protein encoded by plant cell nuclear DNA.
- the transit peptide then separates from the chloroplast protein or is proteolitically removed, during the translocation process of the latter protein into the chloroplasts.
- suitable transit peptides are those associated with the small subunit of ribulose-1,5 biphosphate (RuBP) carboxylase or that associated with the chlorophyl a/b binding proteins.
- the invention also relates to a process, which can be generally defined as a process for producing plants and reproduction material of said plants including a heterologous genetic material stably integrated therein and capable of being expressed in said plants or reproduction material in the form of a protein capable of inactivating or neutralizing the activity of a glutamine synthetase-inhibitor, comprising the non biological steps of producing plants cells or plant tissue including said heterologous genetic material from starting plant cells or plant tissue not able to express that inhibiting or neutralizing activity, regenerating plants or reproduction material of said plants or both from said plant cells or plant tissue including said genetic material and, optionally, biologically replicating said last mentioned plants or reproduction material or both, wherein said non-biological steps of producing said plant cells or plant tissue including said heterologous genetic material, comprises transforming said starting plant cells or plant tissue with a DNA-recombinant containing a nucleotide sequence encoding said protein, as well as the regulatory elements selected among those which are capable of enabling the expression of said nucleotide sequence
- the invention also relates to the cell cultures containing Bialaphos-resistance-DNA, or more generally said GS-inhibitor-inactivating DNA, which cell cultures have the property of being resistant to a composition containing a GS inhibitor, when cultured in a medium containing a such composition at dosages which would be destructive for non transformed cells.
- the invention concerns more particularly those plant cells or cell cultures in which the Bialaphos-resistance DNA is stably integrated and which remains present over successive generations of said plant cells.
- a GS inhibitor more particularly Bialaphos or PPT, can also be considered as a way of characterizing the plant cells of this invention.
- the invention relates to plant cells, reproduction material, particularly seeds, as well as plants containing a foreign or heterologous DNA fragment stably integrated in their respective genomic DNAs, said fragments being transferred throughout generations of such plant cells, reproduction material, seeds and plants, wherein said DNA fragment encodes a protein inducing a non-variety-specific enzymatic activity capable of inactivating or neutralizing GS inhibitors, particularly Bialaphos and PPT, more particularly to confer on said plant cells, reproduction material, seeds and plants a corresponding non-variety-specific phenotype of resistance to GS inhibitors.
- Non-variety-specific enzymatic activity or phenotype aims at referring to the fact that they are not characteristic of specific plant genes or species as this will be illustrated in a non-limitative way by the examples which will follow. They are induced in said plant materials by essentially non-biological processes applicable to plants belonging to species normally unrelated with one another and comprising the incorporation into said plant material of heterologous DNA, e.g. bacterial DNA or chemically synthesized DNA, which does not normally occur in said plant material or which normally cannot be incorporated therein by natural breeding processes, and which yet confers a common phenotype (e.g. herbicide resistance) to them.
- heterologous DNA e.g. bacterial DNA or chemically synthesized DNA
- the invention is of particular advantageous use in processes for protecting field-cultivated plant species against weeds, which processes comprise the step of treating the field with an herbicide, e.g. Bialaphos or PPT in a dosage effective to kill said weeds, wherein the cultivated plant species then contains in their genome a DNA fragment encoding a protein having an enzymatic activity capable of neutralizing or inactivating said GS inhibitor.
- an herbicide e.g. Bialaphos or PPT
- effective doses for use in the abovesaid process range from about 0.4 to about 1.6 kg/Hectare of Bialaphos or PPT.
- the following disclosure also provides the technique which can be applied to other strains producing compounds with a PPT moiety.
- Bialaphos-inactivating-DNA fragment designated thereafter by Bialaphos-resistance gene or “sfr” gene, isolated by the above described technique into plasmids which can be used for transforming plant cells and conferring to them a resistance against Bialaphos, also merely by way of example for non-limitative illustration purposes.
- FIG. 1 is a restriction map of a plasmid containing a Streptomyces hygroscopicus DNA fragment encoding Bialaphos-resistance, which plasmid, designated hereafter as pBG1 has been constructed according to the disclosure which follows;
- FIG. 2 shows the nucleotide sequence (SEQ ID NO:12) of a smaller fragment obtained from pBG1, subcloned into another plasmid (pBG39) and containing the resistance gene;
- FIG. 3 shows the construction of a series of plasmids given by way of example, which plasmids aim at providing suitable adaptation means for the insertion therein of the Bialaphos-resistance gene or “sfr” gene;
- FIGS. 4A and 4B show the construction of a series of plasmids given by way of example, which plasmids contain suitable plant cell promoter sequences able to initiate transcription and expression of the foreign gene inserted under their control into said plasmids;
- FIG. 5A shows a determined fragment of the nucleotide sequence (SEQ ID NO:13) of the plasmid obtained in FIG. 3 ;
- FIG. 5B shows the reconstruction of the first codons of a Bialaphos-resistance gene, from a FokI/BglII fragment obtained from pBG39 and the substitution of an ATG initiation codon for the GTG initiation codon of the natural “sfr” gene (SEQ ID NO:14);
- FIG. 5C shows the reconstruction of the entire “sfr” gene, namely the last codons thereof (SEQ ID NO:15), and its insertion into a plasmid obtained in FIGS. 4A and 4B ;
- FIG. 6A shows an expression vector containing the “sfr” gene placed under the control of a plant cell promoter
- FIG. 6B shows another expression vector deriving from the one shown in FIG. 6A , by the substitution of some nucleotides.
- FIG. 7 shows the construction of a series of plasmids given by way of examples, to ultimately produce plasmids containing the promoter region and the transit peptide sequence of a determined plant cell gene, for the insertion of the “sfr” gene under the control of said promoter region and downstream of said transit peptide sequence (SEQ ID NOS: 16 and 17).
- FIGS. 8 to 11 will be referred to hereafter.
- the following experiment was set up to isolate a Bialaphos-resistance-gene from S. hygroscopicus, according to standard techniques for cloning into Streptomyces.
- lividans strain 66 protoplasts by a transformation procedure mediated by polyethylene-glycol (PEG) as described hereafter. These protoplasts gave rise to 5 ⁇ 10 7 colonies and 4 ⁇ 10 4 pocks after regeneration on 20 plates of R2 agar containing 0.5% of Difco yeast extract (R2 YE). Preparation and composition of the different mediums and buffers used in the disclosed experiments are described hereinafter. When these lawns were replica-plated on minimal medium plates containing 50 ⁇ g ml ⁇ 1 Bialaphos, drug resistant colonies appeared at a frequency of 1 per 10 4 transformants. After purification of the drug resistant colonies, there plasmid DNA was isolated and used to retransform S. lividans protoplasts.
- PEG polyethylene-glycol
- Non selective regeneration followed by replication to Bialaphos-containing-medium demonstrated a 100% correlation between pocks and Bialaphos resistant growth.
- the recombinant plasmids of several resistant clones all contained a 1.7 Kb PstI insert (see FIG. 1 ).
- the 1.7 Kb PstI insert was then subcloned into the high copy number streptomycete vector pIJ385 to generate plasmid pBG20.
- S. lividans strains which contained pBG20 were more than 500 times more resistant to Bialaphos.
- S. lividans growth is normally inhibited in minimal medium containing 1 ⁇ g/ml Bialaphos; growth of transformants containing pBG20 was not noticeably inhibited in a medium containing 500 ⁇ g/ml Bialaphos.
- the PstI fragment was also subcloned in either orientation into the PstI site of the plasmid pBR322, to produce plasmids pBG1 and pBG2, according to their orientation.
- a test on minimal M9 medium demonstrated that E. coli E8767 containing pBG1 or pBG2 was resistant to Bialaphos.
- a ⁇ 1.65 Kb PstI-BamHI fragment was subcloned from pBG1 into the plasmid pUC19 to produce the plasmid pBG39, and conferred Bialaphos resistance to E. coli, W3110, C600 and JM83.
- the 1,65 Kb PstI-BamHI fragment in pBG39 was shown to direct the synthesis of a 22 Kd protein.
- this 1,65 Kb insert includes a fragment coding for a 22 Kd protein and will be called “sfr” gene.
- a 625 bp Sau3A fragment was subcloned from pBG39 into pUC19 and still conferred Bialaphos resistance to a E. coli W3110 host.
- the resulting clones were pBG93 and pBG94, according to the orientation.
- the protein encoded by these clones was detected by using coupled transcription-translation systems derived from extracts of S. lividans (ref. 7). Depending on the orientation of the Sau3A fragment, translation products of different sizes were observed; 22 Kd for pBG94 and ⁇ 28 Kd for pBG93. This indicated that the Sau3A fragment did not contain the entire resistance gene and that a fusion protein was formed which included a polypeptide sequence resulting from the translation of a pUC19 sequence.
- This sequence matched an amino-acid sequence which was deduced from the open reading frame of the 625 bp Sau3A fragment. It corresponded to the stretch from codon 3 to codon 12.
- the NH 2 -terminus of the 22 Kd protein was upstream of this sequence. It was determined that translation of the actual protein was likely to be initiated 2 amino-acids earlier at a GTG initiation codon. GTG is often used as initiator codon in Streptomyces and translated as methionine. The protein translated from the GTG initiation codon would be 183 amino-acids long and would have a molecular weight of 20 550. This was in good agreement with the observed approximate molecular weight of 22 000.
- the termination codon was located just downstream of the Sau3A site. Cloning of the 625 bp Sau3A fragment in a BamHI site digested pUC19 did not result in the reconstruction of the termination codon. This explained the fusion proteins which were observed in the in vitro transcription-translation analysis.
- PPT is the portion of Bialaphos which inhibits glutamine synthetase (GS) and that N-acetyl PPT is not an inhibitor.
- S. hygroscopicus ATCC 21 705 derivates were shown to contain a PPT acetyl transferase which was not found in S. lividans. The activity does not acetylate the Bialaphos tripeptide.
- lividans carrying the resistance gene cloned in pBG20 or pBG16 also contained the activity which could acetylate PPT but not Bialaphos.
- the PPT derived reaction product produced by extracts of pBG20/S. lividans was isolated in order to confirm that it was indeed acetyl-PPT. Analysis by mass spectroscopy showed that the molecular weight had increased relative to PPT by the equivalent of one acetyl group. It was thus concluded that the 625 bp Sau3A fragment contained sequences which code for PPT acetyl transferase.
- 1° P medium 10.3 g of sucrose, 0.025 g of K 2 SO 4 , 0.203 g of MgCl 2 .6H 2 O and 0.2 ml of a trace element solution are dissolved in 80 ml of distilled water and autoclaved. Then in order, 1 ml of KH 2 PO 4 (0.5%), 10 ml of CaCl 2 , 2H 2 O (3.68%), and 10 ml of TES buffer (0.25 M), pH: 7.2) are added.
- Trace element solution (per liter): ZnCl 2 , 40 mg; FeCl 3 .6H 2 O, 200 mg; CuCl 2 .2H 2 O, 10 mg; MnCl 2 .4H 2 O, 10 mg; Na 2 B 4 O 7 .10H 2 O, 10 mg; (NH 4 ) 6 Mo 7 O 24 .4H 2 O, 10 mg.
- a “sfr” gene cassette was constructed to allow subsequent cloning in plant expression vectors.
- the complementary synthetic oligonucleotides were (SEQ ID NOS:5-6) 5′-CATGAGCCCAGAAC and 3′-TCGGGTCTTGCTGC.
- the 5′ end of the “sfr” gene could be reformed and the GTG initiation codon substituted for a codon well translated by plant cells, particularly an ATG codon.
- the DNA fragment containing the oligonucleotides linked to the “sfr” gene was then inserted into an appropriate plasmid, which contained a determined nucleotide sequence thereafter designated by an “adapter” fragment.
- This adapter fragment comprised:
- the “sfr” gene was then inserted into another plasmid, which contained a suitable plant promoter sequence.
- the plant promoter sequence consisted of the cauliflower mosaic virus promoter sequence (p35S).
- p35S cauliflower mosaic virus promoter sequence
- the invention is not limited to the use of this particular promoter.
- Other sequences could be chosen as promoters suitable in plants, for example the TR 1′-2′ promoter region and the promoter fragment of a Rubisco small subunit gene from Argbidopsis thaliana hereafter described.
- plasmid pLK56.2 aimed at obtaining a suitable adaptor including the following sequence of restriction sites: SmaI, BamHI, NcoI, KpnI, BglII, MluI, BamHI, HindIII and XbaI.
- the starting plasmids used for this construction were those disclosed by BOTTERMAN (ref. 11).
- LGA low melting point agarose
- the plasmid pLK56 was cleaved by the enzymes BamHI and NdeI.
- a NcoI-NdeI fragment (referred to in the drawings by arc “a” in broken line) obtained from plasmid pJB64 was substituted in pLK56 for the BamHI-NdeI fragment shown at “b”. Ligation was possible after filling in the BamHI and NcoI protruding ends with the DNA polymerase I of E. coli (Klenow's fragment).
- This plasmid was cleaved by the enzymes XbaI and PstI.
- the obtained plasmid pLK56.2 contained a nucleotide sequence which comprised the necessary restriction sites for the subsequent insertion of the “sfr” gene.
- Plasmid pGV825 is described in DEBLAERE et al. (ref. 10). Plasmid pJB63 is from BOTTERMAN (ref. 11).
- pGV825 was linearized with PvuII and recircularized by the T4 DNA ligase, resulting in the deletion of an internal PvuII fragment shown at (e), (plasmid pGV956).
- pGV956 was then cleaved by BamHI and BglII.
- the BamHI-HindIII fragment (f) obtained from pJB63 was dephosphorylated with calf intestine phosphatase (CIP) and substituted for the BamHI-BglII fragment of pGV956.
- Plasmid pGV1500 was obtained after recircularization by means of T4 DNA ligase.
- This plasmid contains the promoter fragment in front of the 3′ end of the T-DNA transcript 7 and a BamHI and ClaI sites for cloning.
- CP3 is a plasmid derived from pBR322 and which contains the 35S promoter region of cauliflower mosaic virus within a BamHI fragment.
- pGSH150 was cut by BamHI and BglII.
- a BamHI fragment obtained from mGV2 (ref. 12) was inserted in pGSJ250 at the BglII site to form plasmid pGSJ260.
- pLK56.2 was further modified as discussed below to yield pGSR1.
- a second plasmid so modified as to achieve transport of the Bialaphos-resistance enzymes to the chloroplasts of plant cells.
- FIG. 5A SEQ ID NO:13
- the nucleotide sequence of the adapter of pLK56.2 is shown.
- the locations of BamHI, NcoI, BglII restriction sites are shown.
- This adapter fragment was cleaved by the enzymes NcoI and BglII.
- FIG. 5B shows the FokI-BglII fragment (j) obtained from pBG39. The locations of these two restriction sites are shown on FIG. 2 .
- the first codons of the “sfr” gene were reformed, particularly the 5′ end of the gene in which a ATG initiation codon was substituted for the initial GTG codon.
- the plasmid pGSJ260 was then opened by BamHI ( FIG. 5C ) and the BamHI fragment obtained from pGSR1, which contained the entire “sfr” gene, was inserted into pGSJ260.
- the obtained plasmid, pGSR2 contained a pBR322 replicon, a bacterial streptomycin resistance gene (SDM-SP-AD-transferase) and an engineered T-DNA consisting of:
- the chimeric “sfr” gene consisting of:
- the 3′ untranslated region including the polyadenylation signal of T-DNA transcript 7.
- pGSR2 was introduced into Agrobacterium tumefaciens recipient C58ClRif® (pGV2260) according to the procedure described by DEBLAERE et al. (ref. 10).
- This strain was used to introduce the chimeric “sfr” gene in N. tabacum SR 1 plants.
- pGSR2 Two variant plasmids deriving from pGSR2, namely pGSFR280 and pGSFR281, have been constructed. They differ in the untranslated sequence following the transcription initiation site. In pGSR2, this fragment consists of the following sequence (SEQ ID NO:7):
- All plasmids are introduced in Agrobacterium by cointegration in the acceptor plamids pGV2260 yielding the respective plasmids pGSFR1280, pGSFR1281, pGSFR1160 and pGSFR1161.
- nucleotide sequence which contained the “sfr” gene was fused to a DNA sequence encoding a transit peptide so as to enable its transport into chloroplasts.
- a fragment of the “sfr” gene was isolated from the adapter fragment above described and fused to a transit peptide. With synthetic oligonucleotides, the entire “sfr” gene was reconstructed and fused to a transit peptide.
- the plasmid (plasmid pATS3 mentioned below) which contained the nucleotide sequence encoding the transit peptide comprised also the promoter sequence thereof.
- Plasmid pLK57 is from BOTTERMAN, (ref. 11).
- Plasmid pATS3 is a pUC19 clone which contains a 2 Kb EcoRI genomic DNA fragment from Arabidopsis thaliana comprising the promoter region and the transit peptide nucleotide sequence of the gene, the expression thereof is the small subunit of ribulose biphosphate carboxylase (ssu).
- the A. thaliana small subunit was isolated as a 1 500 bp EcoRI-SphI fragment. The SphI cleavage site exactly occurs at the site where the coding region of the mature ssu protein starts.
- Plasmids pLK57 and pATS3 were opened with EcoRI and SphI. After recircularization by means of the T4 DNA ligase, a recombinant plasmid pLKAB1 containing the sequence encoding the transit peptide (Tp) and its promoter region (Pssu) was obtained.
- the N-terminal gene sequence was first modified. Since it was observed that N-terminal gene fusions with the “sfr” gene retain their enzymatic activity, the second codon (AGC) was modified to a GAC, yielding an NcoI site overlapping with the ATG initiator site.
- a new plasmid, pGSSFR2 was obtained. It only differs from pGSR1 ( FIG. 5B ), by that mutation.
- the NcoI-BamHI fragment obtained from pGSFR2 was fused at the SphI end of the transit peptide sequence.
- the “sfr” gene fragment was fused correctly to the ATG initiator of the ssu gene (not shown in figures).
- the Bialaphos-resistance induced in plants by the expression of chimeric genes, when the latter have been transformed with appropriate vectors containing said chimeric genes, has been demonstrated as follows.
- the recombinant plasmids containing the “sfr” gene were introduced separately by mobilization into Agrobacterium strain C58C 1 Rif® (pGV2260) according to the procedure described by DEBLAERE and al., Nucl. Acid. Res., 13, p. 1 477, 1985.
- Recombinant strains containing hybrid Ti plasmides were formed. These strains were used to infect and transform leaf discs of different plant species, according to a method essentially as described by HORSH and al., 1985, Science, vol. 227. Transformation procedure of these different plant species given by way of example, is described thereafter.
- Plants are used 6 to 8 weeks after subculture on medium A 1
- Remaining parts are cut into segments of about 0.25 cm 2 and are placed in the infection medium A 10 (about 12 segments in a 9 cm Petri dish containing 10 ml A 10 ).
- Segments are then infected with 25 ⁇ l per Petri dish of a late log culture of the Agrobacterium strain grown in min A medium.
- Petri dish are incubated for 2 to 3 days at low light intensity.
- medium is removed and replaced by 20 ml of medium A 10 containing 500 mg/l clarofan.
- the leaf discs are placed on medium A 11 containing a selective agent:
- Leaf discs are transferred to fresh medium weekly.
- Potatoes are peeled and washed with water.
- the outer layer is removed (1 to 1.5 cm)
- the central part is cut into discs of about 1 cm 2 and 2 to 3 mm thick.
- Discs are placed on medium C 1 (4 pieces in a 9 cm Petri dish).
- Discs are incubated for 2 days at low light intensity.
- Discs are dried on a filter paper and transferred to medium C 2 with 100 mg/l kanamycin.
- the calli are transferred to medium C 8 containing 50 mg/l kanamycin.
- the calli are transferred to elongation medium C 9 containing 50 mg/l Kanamycin.
- Elongated shoots are separated and transferred to rooting medium C 11 .
- Rooted shoots are propagated on medium C 5 .
- Plants are used 6 weeks after subculture on medium A 1 .
- Midrib is removed from the leaves.
- Leaves are cut in segments of about 0.25 to 1 cm 2 (the edges of the leaves are not wounded, so that only maximum 3 sides of the leaf pieces is wounded).
- Segments are placed in infection medium B 1 (upside down), about 10 segments in a 9 cm Petri dish.
- Segments are then infected wiht 20 ⁇ l per Petri dish of a late log culture of the Agrobacterium strain grown in min A medium.
- Petri dishes incubate for 2 days at low light intensity.
- Medium is removed after 2 days and replaced by 20 ml of medium B 1 containing 500 mg/l clarofan.
- the leaf discs are placed in medium B 2 +50 or 100 mg/l kanamycin.
- calli are transferred to elongation medium B 9 with 50 or 100 mg/l kanamycin.
- Elongated shoots are separated and transferred to medium B 8 for rooting.
- Plants are propagated on medium A 1 .
- Temperature of the greenhouse is about 22° C. for tobaccos and tomato, and above 10° C. to 15° C. for potato.
- Nicotiana tabacum cv. Petit Havana SR1 plants transformed with the chimeric “sfr” genes as present in pGSFR1161 or pGSFR1281, as well as unstransformed control plants (from 10 cm to 50 cm high) are treated with 20 1 BASTA®/ha.
- Control SR1 plants die after 6 days, while transformed plants are fully resistant to 20 1 BASTA®/ha and continue growing undistinguishable from untreated plants. No visible damage is detected, also the treatment is repeated every two weeks. The treatment has no effect in subsequent flowering.
- the recommended dose oF BASTA® herbicide in agriculture is 2.5-7.5 l/ha.
- Untransformed and transformed potato plants (Solanum tuberosum cv. Berolina) (20 cm high) with the chimeric “sfr” gene as present in pGSFR1161 or pGSFR1281 are treated with 20 1 BASTA®/ha. Control plants die after 6 days while the transformed plants do not show any visible damage. They grow undistiguishable from untreated plants.
- Untransformed and transformed tomato plants (lycopersium esculentum c.v. luculus) (25 cm high) with the chimeric “sfr” gene as present in pGSFR1161 and pGSFR1281 are treated with 20 1 BASTA®/ha. control plants die after six days while transformed plants are fully resistant. They do not show any visible damage and grow undistiguishable from untreated plants.
- potato plants expressing chimeric “sfr” genes as present in pGSFR1161 or pGSFR1281 are grown in a greenhouse compartment at 20° C. under high humidity. Plants are innoculated by spraying 1 ml of a suspension of 10 6 Phytophtora infestans spores per ml. Plants grow in growth chambers (20° C., 95% humidity, 4 000 lux) until fungal disease symptoms are visible (one week). One set of the plants are at that moment sprayed with Bialaphos at a dose of 8 l/ha. Two weeks later, untreated plants are completely ingested by the fungus. The growth of the fungus is stopped on the Bialaphos treated plants and no further disease symptoms evolve. The plants are effectively protected by the fungicide action of Bialaphos.
- Transformed tobacco plants expressing the chimeric “sfr” gene present in pGSFR1161 and pGSFR1281 are brought to flowering in the greenhouse. They show a normal fertility.
- F1 seedlings 10 resistant F1 seedlings are grown to maturity and seeds are harvested.
- F2 seedlings are grown as described above and tested for PPT-resistance by spraying BASTA® at a dose of 8 l/ha.
- Some of the F1 plants produce F2 seedlings which are all PPT-resistant showing that these plants are homozygous for the resistance gene.
- the 5 invention also concerns plant cells and plants non-essentially-biologically-transformed with a GS inhibitor-inactivating-gene according to the invention.
- plant cells and plants are non-biologically-transformed with the “sfr” gene hereabove described.
- Such plant cells and plants possess, stably integrated in their genome, a non-variety-specific character which render them able to produce detectable amounts of phosphinotricinacetyl transferase.
- This character confers to the transformed plant cells and plants a non-variety-specific enzymatic activity capable of inactivating or neutralizing GS inhibitors like Bialaphos and PPT.
- plant cells and plants transformed according to the invention are rendered resistant against the herbicidal effects of Bialaphos and related compounds.
- Bialaphos was first described as a fungicide, transformed plants can also be protected against fungal diseases by spraying with the compound several times.
- Bialaphos or related compounds is applied several times, particularly at time intervals of about 20 to 100 days.
- the invention also concerns a new process for selectively protecting a plant species against fungal diseases and selectively destroying weeds in a field comprising the steps of treating a field with an herbicide, wherein the plant species contain in their genome a DNA fragment encoding a protein having an enzymatic activity capable of neutralizing or inactivating GS inhibitors and wherein the used herbicide comprises as active ingredient a GS inhibitor.
- plant species are transformed with a DNA fragment comprising the “sfr” gene as described hereabove, and the used herbicide is PPT or a related compound.
- a solution of PPT or related compound is applied over the field, for example by spraying, several times after emergence of the plant species to be cultivated, until early and late germinating weeds are destroyed.
- fields Before emergence of the desired plant species, fields can be treated with any available herbicide, including Bialaphos-type herbicides.
- Bialaphos or related compound is applied several times.
- the herbicide is applied at time intervals of about from 20 to 100 days.
- plants to be cultivated are transformed in such a way as to resist to the herbicidal effects of Bialaphos-type herbicides, fields can be treated even after emergence of the cultivated plants.
- Bialaphos or related compoud is applied at a dose ranging from about 0.4 to about 1.6 kg/ha, and diluted in a liquid carrier at a concentration such as to enable its application to the field at a rate ranging from about 2 to about 8 l/ha.
- the North European sugarbeet is planted from March 15 up to April 15, depending upon the weather condition and more precisely on the precipitation and average temperature.
- the weeds problems are more or less the same in each country and can cause difficulties until the crop closes its canopy around mid-July.
- pre-emergence herbicides have been successivefully used.
- Such compounds are for example those commercially available under the registered trademarks: PYRAMIN®, GOLTIX® and VENZAR®.
- PYRAMIN® commercially available under the registered trademarks: PYRAMIN®, GOLTIX® and VENZAR®.
- the susceptibility to dry weather conditions of these products as well as the lack of residual activity to control late germinating weeds have led the farmer to use post-emergence products in addition to pre-emergence ones.
- post-emergence herbicides consist of Bialaphos or related compounds, which offer a good level of growth control of annual grasses (Bromus, Avena spp., Alopecurus, POA) and broadleaves (Galium, Polygonum, Senecio, Solanum, Mercurialis).
- Post-emergence herbicides can be applied at different moments of the growth of sugarbeet; at a cotyledon level, two-leave level or at a four-leave level.
- Table (II) thereafter represents possible systems of field-treatment, given by way of example.
- the post-emergence herbicide of the class of Bialaphos used is BASTA®, in combination with different pre-emergence herbicides. Concentrations are indicated in l/ha or kg/ha.
- Potatoes are grown in Europe on about 8.10 6 Ha.
- the major products used for weed control are Linuron/mono-linuron or the compound commercially available under the denomination METRABUZIN
- weeds such as Galium and Solanum plus late germinating Chenopodium and Polygonum are not always effectively controlled, while control of the annual grasses is also sometime erratic.
- Table (III) thereafter represents some examples given by way of example of field-treatment in the case of potatoes.
- strains PGSJ260 and pBG39 used hereabove have been deposited on Dec. 12, 1985, at the “German Collection of Micro-organisms” (DEUTSCHE SAMMLUNG VON MIKROORGANISMEN) at Gottingen, Germany. They received the deposition numbers DSM 3 606 and DSM 3 607 respectively.
- FIG. 8 shows the restriction map of a plasmid pJS1 containing another Bialaphos-resistance-gene
- FIG. 9 shows the nucleotide sequence of the “sfrsv” gene containing the resistance gene
- FIG. 10 shows the amino acid homology of “sfrsv” gene and “sfr” gene
- FIG. 11 shows the construction of a plasmid, given by way of example, which contains the “sfrsv” gene and suitable for the transformation of plant cells.
- Bialaphos-resistance-gene has been isolated form another Bialaphos-producing-strains, i.e. streptomyces viridochromogenes. This second resistance-gene is thereafter designated by “sfrsv” gene.
- This second preferred DNA fragment according to the invention for the subsequent transformation of plant cells, consists of a nucleotide sequence (SEQ ID NO:11) coding for at least part of a polypeptide having the following sequence:
- This second preferred DNA fragment consists of the following nucleotide sequence (SEQ ID NO:12):
- the strain Streptomyces viridochromogenes DSM 40736 (ref 1) was grown and total DNA of this strain was prepared according to standard techniques. DNA samples were digested respectively with PstI, SmaI and Sau3AI in three different reactions and separated on an agarose gel, together with plasmid DNA from pGSR1 ( FIG. 5B ) digested with BamHI. In a Southern analysis the DNA was blotted on a nitrocellulose filter and hybridized with the labbeled BamHI fragment from pGSR1 containing the “sfr” gene.
- a restriction fragment was showing strong homology with the probe: a PstI fragment of about 3 kb, a SmaI fragment of about 1.2 kb and Sau3AI fragment of 0.5 kb.
- PstI restriction fragments were directly cloned in the Escherichia coli vector pUC8. 3000 colonies obtained after transformation were transferred to nitrocellulose filters, and hybridized with the “sfr” probe. Positive candidates were further tested for their growth on minimal medium plates containing 300 ⁇ g/ml PPT. One transformant that grew on PPT-containing-medium was further analysed.
- the plasmid map and relevant restriction sites of this plasmid pJS1 are represented in FIG. 8 .
- the strain MC1061 (pJS1) has been deposited on Mar. 6, 1987 at the DEUTSCHE SAMMLUNG VON MIKROORGANISMEN (DSM) under deposition number DSM 4023.
- the clone restriction fragment has been sequenced according to the Maxam and Gilbert method and the coding region of the gene could be identified through homology.
- the sequence of the “sfrsv” gene is represented in FIG. 9 and the homology on the nucleotide and amino acid sequence level with “sfr” gene is shown in FIG. 10 .
- a “sfrsv gene cassette” was also constructed to allow subsequent cloning in plant expression vectors.
- a BanII-BglII fragment containing the “sfrsv” coding region without the initiation codon GTG was isolated from PJS1. This fragment was ligated in the vector pLK56-2 digested with NcoI and BglII, together with a synthetic oligonucleotide 5′-CATGAGCC-3′, similar with the one described for “sfr” gene and shown in FIG. 5 .
- the construction of pGSR1SV is schematically shown in FIG. 11 . Since similar cassettes of both genes are present in respectively pGSR1 and pGSR1SV, previous described constructions for the expression of the “sfr” gene in plants can be repeated.
- Enzymatic analysis of crude extracts from E. coli strains carrying plasmid pGSR1SV demonstrated the synthesis of an acetylase which could acetylate PPT. This was shown by thin layer chromotography of the reaction porducts.
- the “sfrsv” gene was then inserted into the plasmid vector pGSJ260 ( FIG. 4B ) under the control of the CaMV 35s promoter, to yield a plasmid pGS2SV, similar to pGSR2 ( FIG. 6A ) except that the “sfrsv” gene is substituted for the “sfr” gene.
- herbicide resistance genes of the above type may be obtained from many other microorganisms that produce PPT or PPT derivatives. Herbicide resistance gene can then be incorporated in plant cells with a view of protecting them against the action of such Glutamine Synthetase inhibitors.
- a Bialaphos-resistance-gene is obtained from Kitasotosporia (ref. 15).
- Transformed plant cells and plants which contain the “sfrsv” resistance gene can be obtained with plasmid pGSR2SV, using the same Agrobacterium-mediated-transformation system as hereabove described for the transformation of different plant species with the “sfr” gene.
- Plants are regenerated and tested for their resistance with similar spraying tests as described hereabove. All plants behaved similarly and show resistance against herbicides consisting of glutamine synthetase inhibitors.
- the inventors also pertains to the combination of the plants resistant to an inhibitor of glutamine synthetase as defined above with the corresponding inhibitor of glutamine synthetase for use in the production of cultures of said plants free form weeds.
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
The invention relates to a DNA fragment containing a determined gene, the expression of which inhibits the antibiotic and herbicidal effects of Bialaphos and related products.
It also relates to recombinant vectors, containing such DNA fragment, which enable this protective gene to be introduced and expressed into cells and plant cells.
Description
This application is a divisional of application Ser. No. 07/525,300, filed May 17, 1990, now U.S. Pat. No. 5,561,236, which is a continuation of application Ser. No. 07/131,140, filed Nov. 5, 1987, abandoned, which is a continuation under 35 USC §§363 and 120 of PCT/EP 87/00141, filed Mar. 11, 1987, published as WO 87/05629.
The invention relates to a process for protecting plant cells and plants against the action of glutamine synthetase inhibitors.
It also relates to applications of such process, particularly to the development of herbicide resistance into determined plants.
It relates further to non-biologically transformed plant cells and plants displaying resistance to glutamine synthetase inhibitors as well as to suitable DNA fragments and recombinants containing nucleotide sequences encoding resistance to glutamine synthetase inhibitors.
Glutamine synthetase (hereafter simply designated by GS) constitutes in most plants one of the essential enzymes for the development and life of plant cells. It is known that GS converts glutamate into glutamine. GS is involved in an efficient pathway (the only one known nowadays) in most plants for the detoxification of ammonia released by nitrate reduction, aminoacid degradation or photorespiration. Therefore potent inhibitors of GS are very toxic to plant cells. A particular class of herbicides has been developped, based on the toxic effect due to inhibit inhibition of GS in plants.
These herbicides comprise as active ingredient a GS inhibitor.
There are at least two possible ways which might lead to plants resistant to the inhibitors of the action of glutamine synthetase; (1) by changing the target. It can be envisaged that mutations in the GS enzyme can lead to insensitivity towards the herbicide; (2) by inactivation of the herbicide. Breakdown or modification of the herbicide inside the plant could lead to resistance.
Bialaphos and phosphinothricin (hereafter simply designated by PPT) are two such inhibitors of the action of GS, (ref. 16, 17) and have been shown to possess excellent herbicidal properties (see more particularly ref. 2 as concerns Bialaphos).
Bialaphos has the following formula (I)
PPT has the following formula (II)
Thus the structural difference between PPT and Bialaphos resides in the absence of two alanine aminoacids in the case of PPT.
These two herbicides are non selective. They inhibit growth of all the different species of plants present on the soil, accordingly cause their total destruction.
Bialaphos was first disclosed as having antibiotic properties, which enabled it to be used as a pesticide or a fungicide. Bialaphos can be produced according to the process disclosed in U.S. Pat. No. 3,832,394, assigned to MEIJI SEIKA KAISHA LTD., which patent is incorporated herein by reference. It comprises cultivating Streptomyces hygroscopicus, such as the strain available at the American Type Culture Collection, under the ATCC number 21,705, and recovering Bialaphos from its culture medium. However, other strains, such as Streptomyces viridochromogenes, also produce this compound (ref. 1).
Other tripeptide antibiotics which contain a PPT moiety are or might be discovered in nature as well, e.g. phosalacin (ref. 15).
PPT is also obtained by chemical synthesis and is commercially distributed by the industrial Company HOECHST.
A number of Streptomyces species have been disclosed which produce highly active antibiotics which are known to incapacitate procaryotic cell functions or enzymes. The Streptomyces species which produce these antibiotics would themselves be destroyed if they had not a self defence mechanism against these antibiotics. This self defence mechanism has been found in several instances to comprise an enzyme capable of inhibiting the antibiotic effect, thus of avoiding autotoxicity for the Streptomyces species concerned. This modification is generally reversed when the molecule is exported from the cell.
The existence of a gene which encodes an enzyme able to modify the antibiotic so as to inhibit the antibiotic effect against the host has been demonstrated in several Streptomyces producing antibiotics, for example, in S. fradiae, S. azureus, S. vinaceus, S. erythreus, producing neomycin, thiostrepton, viomycin, and MLS (Macrolide Lincosamide Streptogramin) antibiotics respectively (ref. 4), (ref. 5), (ref. 6),(ref. 14 by CHATER et al., 1982 describes standard techniques which can be used for bringing these effects to light).
In accordance with the present invention, it has been found that Streptomyces hygroscopicus ATCC 21,705, also possesses a gene encoding an enzyme responsible of the inactivation of the antibiotic properties of Bialaphos. Experiments carried out by the applicants have lead to the isolation of such a gene and its use in a process for controlling the action of GS inhibitors, based on PPT or derived products.
An object of the invention is to provide a new process for controlling the action in plant cells and plants of GS inhibitors.
Another object of the invention is to provide DNA fragments and DNA recombinants, particularly modified vectors containing said DNA fragments, which DNA fragments contain nucleotide sequences capable, when incorporated in plant cells and plants, to protect them against the action of GS inhibitors.
A further object of the invention is to provide non-biologically transformed plant cells and plants capable of neutralizing or inactivating GS inhibitors.
A further object of the invention is to provide a process for selectively protecting plant species against herbicides of a GS inhibitor type.
More specifically an object of the invention is to provide a DNA fragment transferable to plant cells- and to whole plants—capable of protecting them against the herbicidal effects of Bialaphos and of structurally analogous herbicides.
A further object of the invention is to provide plant cells resistant to the products of the class examplified by Bialaphos, which products possess the PPT unit in their structure.
The process according to the invention for controlling the action in plant cells and plants of a GS inhibitor when contacted therewith, comprises providing said plants with a heterologous DNA fragment including a foreign nucleotide sequence, capable of being expressed in the form of a protein in said plant cells and plants, under condition such as to cause said heterologus DNA fragment to be integrated stably through generations in the cells of said plants, and wherein said protein has an enzymatic activity capable of inactivating or neutralization of said glutamine synthetase inhibitor.
A preferred DNA fragment is one derived from an antibiotic-producing-Streptomyces strain (or a sequence comprising a nucleotide sequence encoding the same activity) and which encodes resistance to a said GS inhibitors.
Preferred nucleotide sequences for use in this invention encode a protein which has acetyl tranferase activity with respect to said GS inhibitors.
A most preferred DNA fragment according to the invention comprises a nucleotide sequence coding for a polypeptide having a PPT acetyl transferase activity.
A particular DNA fragment according to the invention, for the subsequent transformation of plant cells, consists of a nucleotide sequence coding for at least part of a polypeptide having the following sequence (SEQ ID NO:1):
X SER PRO GLU | |
183 | |
ARG ARG PRO ALA ASP ILE ARG ARG ALA THR GLU ALA ASP MET PRO | |
228 | |
ALA VAL CYS THR ILE VAL ASN HIS TYR ILE GLU THR SER THR VAL | |
273 | |
ASN PHE ARG THR GLU PRO GLN GLU PRO GLN GLU TRP THR ASP ASP | |
318 | |
LEU VAL ARG LEU ARG GLU ARG TYR PRO TRP LEU VAL ALA GLU VAL | |
363 | |
ASP GLY GLU VAL ALA GLY ILE ALA TYR ALA GLY PRO TRP LYS ALA | |
408 | |
ARG ASN ALA TYR ASP TRP THR ALA GLU SER THR VAL TYR VAL SER | |
453 | |
PRO ARG HIS GLN ARG THR GLY LEU GLY SER THR LEU TYR THR HIS | |
498 | |
LEU LEU LYS SER LEU GLU ALA GLN GLY PHE LYS SER VAL VAL ALA | |
543 | |
VAL ILE GLY LEU PRO ASN ASP PRO SER VAL ARG MET HIS GLU ALA | |
588 | |
LEU GLY TYR ALA PRO ARG GLY MET LEU ARG ALA ALA GLY PHE LYS | |
633 | |
HIS GLY ASN TRP HIS ASP VAL GLY PHE TRP GLN LEU ASP PHE SER | |
678 | |
LEU PRO VAL PRO PRO ARG PRO VAL LEU PRO VAL THR GLU ILE | |
723 |
in which X represents MET or VAL, which part of said polypeptide is of sufficient length to confer protection against Bialaphos to plant cells, when incorportated genetically and expressed therein, i.e. as termed hereafter “plant-protecting capability” against Bialaphos.
A preferred DNA fragment consists of the following nucleotide sequence (SEQ ID NO:2):
GTG AGC CCA GAA | |
183 | |
CGA CGC CCG GCC GAC ATC CGC CGT GCC ACC GAG GCG GAC ATG CCG | |
228 | |
GCG GTC TGC ACC ATC GTC AAC CAC TAC ATC GAG ACA AGC ACG GTC | |
273 | |
AAC TTC CGT ACC GAG CCG CAG GAA CCG CAG GAG TGG ACG GAC GAC | |
318 | |
CTC GTC CGT CTG CGG GAG CGC TAT CCC TGG CTC GTC GCC GAG GTG | |
363 | |
GAC GGC GAG GTC GCC GGC ATC GCC TAC GCG GGC CCC TGG AAG GCA | |
408 | |
CGC AAC GCC TAC GAC TGG ACG GCC GAG TCG ACC GTG TAC GTC TCC | |
453 | |
CCC CGC CAC CAG CGG ACG GGA CTG GGC TCC ACG CTC TAC ACC CAC | |
498 | |
CTG CTG AAG TCC CTG GAG GCA CAG GGC TTC AAG AGC GTG GTC GCT | |
543 | |
GTC ATC GGG CTG CCC AAC GAC CCG AGC GTG CGC ATG CAC GAG GCG | |
588 | |
CTC GGA TAT GCC CCC CGC GGC ATG CTG CGG GCG GCC GGC TTC AAG | |
633 | |
CAC GGG AAC TGG CAT GAC GTG GGT TTC TGG CAG CTG GAC TTC AGC | |
678 | |
CTG CCG GTA CCG CCC CGT CCG GTC CTG CCC GTC ACC GAG ATC | |
723 |
or of a part thereof expressing a polypeptide having plant-protecting capability against Bialaphos.
The invention also relates to any DNA fragment differing from the preferred one indicated hereabove by the replacement of any of its nucleotides by others, yet without modifying the genetic information of the preferred DNA sequence mentioned hereabove (normally within the meaning of the universal genetic code), and furthermore to any equivalent DNA sequence which would encode a polypeptide having the same properties, particularly a Bialaphos-resistance-activity.
It will be understood that the man skilled in the art should be capable of readily assessing those parts of the nucleotide sequences that could be removed from either side of any of the DNA fragments according to the invention, for instance by removing terminal parts on either side of said DNA fragment, such as by an exonucleolytic enzyme, for instance Bal31, by recloning the remaining fragment in a suitable plasmid and by assaying the capacity of the modified plasmid to transform appropriate cells and to protect it against the Bialaphos antibiotic or herbicide as disclosed later, whichever assay is appropriate.
For the easiness of language, these DNA fragments will be termed hereafter as “Bialaphos-resistance DNA”. In a similar manner, the corresponding polypeptide will be termed as “Bialaphos-resistance enzyme”.
While in the preceding discussion particular emphasis has been put on DNA fragments capable, when introduced into plant cells and plants, to confer on them protection against Bialaphos or PPT, it should be understood that the invention should in no way be deemed as limited thereto.
In a same manner, the invention pertains to DNA fragments which, when introduced into such plant cells, would also confer on them a protection against other GS inhibitors, for instance of intermediate products involved in the natural biosynthesis of phosphinotricin, such as the compounds designated by the abbreviations MP101 (III), MP102 (IV), the formula of which are indicated hereafter:
More generally, the invention has opened the route to the production of DNA fragments which, upon proper incorporation into plant cells and plants, can protect them against GS inhibitors when contacted therewith, as this will be shown in a detailed manner in relation to Bialaphos and PPT in the examples which will follow.
This having been established, it will be appreciated that any fragment encoding an enzymatic activity which would protect plant cells and plants against said GS inhibitors, by inactivationg, should be viewed as an equivalent of the preferred fragments which have been disclosed hereabove. This would apply especially to any DNA fragments that would result from genetic screening of the genomic DNAs of strains, particularly of antibiotic-producing strains, likely to possess genes which, even-though structurally different, would encode similar activity with respect to Bialaphos or PPT, or even with respect to other GS inhibitors. This applies to any gene in other strains producing a PPT derivative.
Therefore, it should be understood that the language “Bialaphos-resistance DNA” or “Bialaphos-resistance enzyme” used thereafter as a matter of convenience is intended to relate not only to the DNAs and enzymes specifically concerned with resistance to PPT or most directly related derivatives, but more generally with other DNAs and enzymes which would be capable, under the same circumstances, of inactivating the action in plants of GS inhibitors.
The invention also relates to DNA recombinants containing the above defined Bialaphos-resistance DNA fragments recombined with heterologous DNA, said heterologous DNA containing regulation elements and said Bialaphos-resistance DNA being under the control of said regulation elements in such manner as to be expressible in a foreign cellular environment compatible with said regulation elements. Particularly the abovesaid Bialaphos-resistance-DNA fragments contained in said DNA recombinants are devoid of any DNA region involved in the biosynthesis of Bialaphos, when said Bialaphos-resistance-DNA fragment originate themselves from Bialaphos-producing strains.
By “heterologous DNA” is meant a DNA of an other origin than that from which said Bialaphos-resistance-DNA originated, e.g. is different from that of a Streptomyces hygroscopicus or Streptomyces viridochromogenes or even more preferably a DNA foreign to Streptomyces DNA. Particularly said regulation elements are those which are capable of controlling the transcription and translation of DNA sequences normally associated with them in said foreign environment. “Cellular” refers both to microorganisms and to cell cultures.
This heterologous DNA may be a bacterial DNA, particularly when it is desired to produce a large amount of the recombinant DNA, such as for amplification purposes. In that respect a preferred heterologous DNA consists of DNA of E. coli or of DNA compatible with E. coli. It may be DNA of the same origin as that of the cells concerned or other DNA, for instance viral or plasmidic DNA known as capable of replicating in the cells concerned.
Preferred recombinant DNA contains heterologous DNA compatible with plant cells, particularly Ti-plasmid DNA.
Particularly preferred recombinants are those which contain GS inhibitor inactivating DNA under the control of a promoter recognized by plant cells, particularly those plant cells on which inactivation of GS inhibitors is to be conferred.
Preferred recombinants according to the invention further relate to modified vectors, particularly plasmids, containing said GS-inhibitor-inactivating DNA so positioned with respect to regulation elements, including particularly promoter elements, that they enable said GS inhibitor-inactivating DNA to be transcribed and translated in the cellular environment which is compatible with said heterologous DNA. Advantageous vectors are those so engineered as to cause stable incorporation of said GS inhibitor inactivating DNA in foreign cells, particularly in their genomic DNA. Preferred modified vectors are those which enable the stable transformation of plant cells and which confer to the corresponding cells, the capability of inactivating GS inhibitors.
It seems that, as described later, the initiation codon of the Bialaphos-resistance-gene of the Streptomyces hygroscopicus strain used herein is a GTG codon. But in preferred recombinant DNAs or vectors, the Bialaphos-resistance-gene is modified by substitution of an ATG initiation codon for the initiation codon GTG, which ATG enables translation initiation in plant cells.
In the example which follows, the plant promoter sequence which has been used was constituted by a promoter of the 35 S cauliflower mosaic virus. Needless to say that the man skilled in the art will be capable of selecting other plant promoters, when more appropriate in relation to the plant species concerned.
According to an other preferred embodiment of the invention, particularly when it is desired to achieve transport of the enzyme encoded by the Bialaphos-resistance-DNA into the chloroplasts, the heterologous DNA fragment is fused to a gene or DNA fragment encoding a transit peptide, said last mentioned fragment being then intercalated between the GS inhibitor inactivating gene and the plant promoter selected.
As concerns means capable of achieving such constructions, reference can be made to the following British applications 84 32757 filed on Dec. 28, 1984 and 85 00336 filed on Jan. 7, 1985 and to the related applications filed in the United-States of America (No. 06/755,173, filed on Jul. 15, 1985), in the European Patent Office (No. 85 402596.2, filed on Dec. 20, 1985), in Japan (No. 299 730, filed on Dec. 27, 1985), in Israel (No. 77 466 filed on Dec. 27, 1985) and in Australia (No. 5 165 485, filed on Dec. 24, 1985), all of which are incorporated herein by reference.
Reference can also be made to the scientific literature, particularly to the following articles:
VAN DEN BROECK et al., 1985, Nature, 313, 358-363;
SCHREIER and al., Embo. J., vol. 4, No. 1, 25-32.
These articles are also incorporated herein by reference.
For the sake of the record, be it recalled here that under the expression “transit peptide”, one refers to a polypeptide fragment which is normally associated with a chloroplast protein or a chloroplast protein sub-unit in a precursor protein encoded by plant cell nuclear DNA. The transit peptide then separates from the chloroplast protein or is proteolitically removed, during the translocation process of the latter protein into the chloroplasts. Examples of suitable transit peptides are those associated with the small subunit of ribulose-1,5 biphosphate (RuBP) carboxylase or that associated with the chlorophyl a/b binding proteins.
There is thus provided DNA fragments and DNA recombinants which are suitable for use in the process defined hereafter.
More particularly the invention also relates to a process, which can be generally defined as a process for producing plants and reproduction material of said plants including a heterologous genetic material stably integrated therein and capable of being expressed in said plants or reproduction material in the form of a protein capable of inactivating or neutralizing the activity of a glutamine synthetase-inhibitor, comprising the non biological steps of producing plants cells or plant tissue including said heterologous genetic material from starting plant cells or plant tissue not able to express that inhibiting or neutralizing activity, regenerating plants or reproduction material of said plants or both from said plant cells or plant tissue including said genetic material and, optionally, biologically replicating said last mentioned plants or reproduction material or both, wherein said non-biological steps of producing said plant cells or plant tissue including said heterologous genetic material, comprises transforming said starting plant cells or plant tissue with a DNA-recombinant containing a nucleotide sequence encoding said protein, as well as the regulatory elements selected among those which are capable of enabling the expression of said nucleotide sequence in said plant cells or plant tissue, and to cause the stable integration of said nucleotide sequence in said plant cells and tissue, as well as in the plant and reproduction material processed therefrom throughout generations.
The invention also relates to the cell cultures containing Bialaphos-resistance-DNA, or more generally said GS-inhibitor-inactivating DNA, which cell cultures have the property of being resistant to a composition containing a GS inhibitor, when cultured in a medium containing a such composition at dosages which would be destructive for non transformed cells.
The invention concerns more particularly those plant cells or cell cultures in which the Bialaphos-resistance DNA is stably integrated and which remains present over successive generations of said plant cells. Thus the resistance to a GS inhibitor, more particularly Bialaphos or PPT, can also be considered as a way of characterizing the plant cells of this invention.
Optionally one may also resort to hybridization experiments between the genomic DNA obtained from said plant cells with a probe containing a GS inhibitor inactivating DNA sequence.
More generally the invention relates to plant cells, reproduction material, particularly seeds, as well as plants containing a foreign or heterologous DNA fragment stably integrated in their respective genomic DNAs, said fragments being transferred throughout generations of such plant cells, reproduction material, seeds and plants, wherein said DNA fragment encodes a protein inducing a non-variety-specific enzymatic activity capable of inactivating or neutralizing GS inhibitors, particularly Bialaphos and PPT, more particularly to confer on said plant cells, reproduction material, seeds and plants a corresponding non-variety-specific phenotype of resistance to GS inhibitors.
“Non-variety-specific” enzymatic activity or phenotype aims at referring to the fact that they are not characteristic of specific plant genes or species as this will be illustrated in a non-limitative way by the examples which will follow. They are induced in said plant materials by essentially non-biological processes applicable to plants belonging to species normally unrelated with one another and comprising the incorporation into said plant material of heterologous DNA, e.g. bacterial DNA or chemically synthesized DNA, which does not normally occur in said plant material or which normally cannot be incorporated therein by natural breeding processes, and which yet confers a common phenotype (e.g. herbicide resistance) to them.
The invention is of particular advantageous use in processes for protecting field-cultivated plant species against weeds, which processes comprise the step of treating the field with an herbicide, e.g. Bialaphos or PPT in a dosage effective to kill said weeds, wherein the cultivated plant species then contains in their genome a DNA fragment encoding a protein having an enzymatic activity capable of neutralizing or inactivating said GS inhibitor.
By way of illustration only, effective doses for use in the abovesaid process range from about 0.4 to about 1.6 kg/Hectare of Bialaphos or PPT.
There follows now a disclosure of how the preferred DNA fragment described hereabove was isolated starting from the Streptomyces hygroscopicus strain available at the American Type Culture Collection under deposition number ATCC 21 705, by way of exemplification only.
The following disclosure also provides the technique which can be applied to other strains producing compounds with a PPT moiety.
The disclosure will then be completed with the description of the insertion of a preferred DNA fragment conferring to the transformed cells the capability of inactivating Bialaphos and PPT. Thus the Bialaphos-inactivating-DNA fragment designated thereafter by Bialaphos-resistance gene or “sfr” gene, isolated by the above described technique into plasmids which can be used for transforming plant cells and conferring to them a resistance against Bialaphos, also merely by way of example for non-limitative illustration purposes.
The following disclosure is made with reference to the drawings in which:
The following experiment was set up to isolate a Bialaphos-resistance-gene from S. hygroscopicus, according to standard techniques for cloning into Streptomyces.
2.5 μg of S. hygroscopicus genomic DNA and 0.5 μg of Streptomyces vector pIJ61 were cleaved with PstI according to the method described in ref. 6. The vector, fragments and genomic fragments were mixed and ligated (4 hours at 10° C. followed by 72 hours at 4° C. in ligation salts which contain 66 mM Tris-HCl (pH 7.5), 1 mM EDTA, 10 mM MgCl2, 10 mM 2-mercaptoethanol and 0.1 mM ATP) at a total DNA concentration of 40 μg ml−1 with T4 DNA ligase. Ligation products were introduced into 3×109 S. lividans strain 66 protoplasts by a transformation procedure mediated by polyethylene-glycol (PEG) as described hereafter. These protoplasts gave rise to 5×107 colonies and 4×104 pocks after regeneration on 20 plates of R2 agar containing 0.5% of Difco yeast extract (R2 YE). Preparation and composition of the different mediums and buffers used in the disclosed experiments are described hereinafter. When these lawns were replica-plated on minimal medium plates containing 50 μg ml−1 Bialaphos, drug resistant colonies appeared at a frequency of 1 per 104 transformants. After purification of the drug resistant colonies, there plasmid DNA was isolated and used to retransform S. lividans protoplasts. Non selective regeneration followed by replication to Bialaphos-containing-medium demonstrated a 100% correlation between pocks and Bialaphos resistant growth. The recombinant plasmids of several resistant clones all contained a 1.7 Kb PstI insert (see FIG. 1 ).
Subcloning of the Herbicide Resistance Gene
The 1.7 Kb PstI insert was then subcloned into the high copy number streptomycete vector pIJ385 to generate plasmid pBG20. S. lividans strains which contained pBG20 were more than 500 times more resistant to Bialaphos. S. lividans growth is normally inhibited in minimal medium containing 1 μg/ml Bialaphos; growth of transformants containing pBG20 was not noticeably inhibited in a medium containing 500 μg/ml Bialaphos. The PstI fragment was also subcloned in either orientation into the PstI site of the plasmid pBR322, to produce plasmids pBG1 and pBG2, according to their orientation. A test on minimal M9 medium demonstrated that E. coli E8767 containing pBG1 or pBG2 was resistant to Bialaphos.
A ±1.65 Kb PstI-BamHI fragment was subcloned from pBG1 into the plasmid pUC19 to produce the plasmid pBG39, and conferred Bialaphos resistance to E. coli, W3110, C600 and JM83.
Using an in vitro coupled transcription-translation system (ref. 5) from S. lividans extracts, the 1,65 Kb PstI-BamHI fragment in pBG39 was shown to direct the synthesis of a 22 Kd protein. In the following, this 1,65 Kb insert includes a fragment coding for a 22 Kd protein and will be called “sfr” gene.
Fine Mapping and Sequencing of the Gene
A 625 bp Sau3A fragment was subcloned from pBG39 into pUC19 and still conferred Bialaphos resistance to a E. coli W3110 host. The resulting clones were pBG93 and pBG94, according to the orientation.
The orientation of the gene in the Sau3A fragment was indicated by experiments which have shown that Bialaphos resistance could be induced with IPTG from the pUC19 lac promoter in pBG93. In the presence of IPTG (0.5 mM) the resistance of pBG93/W3110 increased from 5 to 50 μg/ml on a M9 medium containing Bialaphos. The W3110 host devoid of pBG93, did not grow on M9 medium containing 5 μg/ml Bialaphos. These experiments demonstrated that the Sau3A fragment could be subcloned without loss of activity. They also provided for the proper orientation as shown in the FIG. 2 , enclosed thereafter. The protein encoded by these clones was detected by using coupled transcription-translation systems derived from extracts of S. lividans (ref. 7). Depending on the orientation of the Sau3A fragment, translation products of different sizes were observed; 22 Kd for pBG94 and ±28 Kd for pBG93. This indicated that the Sau3A fragment did not contain the entire resistance gene and that a fusion protein was formed which included a polypeptide sequence resulting from the translation of a pUC19 sequence.
In order to obtain large amounts of the protein, a 1.7 Kb PstI fragment from pBG1 was cloned into the high copy number Streptomycete replicon pIJ385. The obtained plasmid, pBG20, was used to transform S. hygroscopicus. Transformants which contained this plasmid had more than 5 times as much PPT acetylating activity and also had increased amounts of a 22 kd protein on sodium dodecylsulfate gels (SDS gels). Furthermore, both the acetyl transferase and the 22 kd protein appeared when the production of Bialaphos begun. The correlation of the in vitro data, kinetics of synthesis, and amplified expression associated with pBG20 transformants strongly implied that this 22 Kd band was the gene product.
The complete nucleotide sequence of the 625 bp Sau3A fragment was determined as well as of flanking sequences. Computer analysis revealed the presence of an open reading frame over the entire length of the Sau3A fragment.
Characterization of the sfr Gene Product
A series of experiments were performed to determine that the open reading frame of the “sfr” gene indeed encoded the Bialaphos resistance enzyme. To determine the 5′ end of the resistance gene, the NH2-terminal sequence of the enzyme was determined. As concerns more particularly the technique used to determine the said sequence, reference is made to the technique developed by J. VANDEKERCKHOVE, Eur. J. Bioc. 152, p. 9-19, 1985, and to French patent applications No. 85 14579 filed on Oct. 1, 1985 and No. 85 13046 filed on September 2, 1985, all of which are incorporated herein by reference.
This technique allows the immobilization on glass fibre sheets coated with the polyquaternary amine commercially available under the registered trademark POLYBRENE of proteins and of nucleic acids previously separated on a sodium dodecylsulfate containing polyacrylamide gel. The transfer is carried out essentially as for the protein blotting on nitrocellulose membranes (ref. 8). This allows the determination of amino-acid composition and partial sequence of the immobilized proteins. The portion of the sheet carrying the immobilized 22 kd protein produced by S. hygroscopicus pBG20 was cut out and the disc was mounted in the reaction chambre of a gas-phase sequenator to subject the glass-fibre bound 22 Kd protein to the Edman degradation procedure. The following amino-acid sequence was obtained SEQ ID NO:4):
Pro-Glu-Arg-Arg-Pro-Ala-Asp-Ile-Arg-Arg
This sequence matched an amino-acid sequence which was deduced from the open reading frame of the 625 bp Sau3A fragment. It corresponded to the stretch from codon 3 to codon 12.
Thus, the NH2-terminus of the 22 Kd protein was upstream of this sequence. It was determined that translation of the actual protein was likely to be initiated 2 amino-acids earlier at a GTG initiation codon. GTG is often used as initiator codon in Streptomyces and translated as methionine. The protein translated from the GTG initiation codon would be 183 amino-acids long and would have a molecular weight of 20 550. This was in good agreement with the observed approximate molecular weight of 22 000.
Furthermore, the termination codon, TGA, was located just downstream of the Sau3A site. Cloning of the 625 bp Sau3A fragment in a BamHI site digested pUC19 did not result in the reconstruction of the termination codon. This explained the fusion proteins which were observed in the in vitro transcription-translation analysis.
Mechanism of PPT-Resistance
Having defined a first phenotype and some of the physical characteristics of the resistance gene and its gene product, a series of experiments was then carried out to understand the mechanism by which it confers resistance. As described hereabove, PPT is the portion of Bialaphos which inhibits glutamine synthetase (GS) and that N-acetyl PPT is not an inhibitor. Using a standard assay (ref. 9), S. hygroscopicus ATCC 21 705 derivates were shown to contain a PPT acetyl transferase which was not found in S. lividans. The activity does not acetylate the Bialaphos tripeptide. S. lividans carrying the resistance gene cloned in pBG20 or pBG16 (a plasmid containing the 625 bp Sau3A fragment cloned into another streptomycete vector, pIJ680) also contained the activity which could acetylate PPT but not Bialaphos. The PPT derived reaction product produced by extracts of pBG20/S. lividans was isolated in order to confirm that it was indeed acetyl-PPT. Analysis by mass spectroscopy showed that the molecular weight had increased relative to PPT by the equivalent of one acetyl group. It was thus concluded that the 625 bp Sau3A fragment contained sequences which code for PPT acetyl transferase.
The experimental conditions and reagents used in the techniques disclosed hereabove were as follows:
Preparation and Composition of the Mediums and Buffers Above Used
1° P medium: 10.3 g of sucrose, 0.025 g of K2SO4, 0.203 g of MgCl2.6H2O and 0.2 ml of a trace element solution are dissolved in 80 ml of distilled water and autoclaved. Then in order, 1 ml of KH2PO4 (0.5%), 10 ml of CaCl2, 2H2O (3.68%), and 10 ml of TES buffer (0.25 M), pH: 7.2) are added. Trace element solution (per liter): ZnCl2, 40 mg; FeCl3.6H2O, 200 mg; CuCl2.2H2O, 10 mg; MnCl2.4H2O, 10 mg; Na2B4O7.10H2O, 10 mg; (NH4)6Mo7O24.4H2O, 10 mg.
2° R2YE: 10.3 g of sucrose, 0.025 g of K2SO4, 1.012 g of MgCl2.6H2O, 1 g of glucose, 0.01 g of Difco 25 casamino acids, and 2.2 g of Difco agar are dissolved in 80 ml distilled water and autoclaved. 0.2 ml of trace element solution, 1 ml of KH2PO4 (0.5%), 8.02 ml of CaCl2.2H2O (3.68%), 1.5 ml of L-proline (20%), 10 ml of TES buffer (0.25 M) (pH: 7.2), 0.5 ml of (1 M) NaOH, 5 ml of yeast extract (10%) are sequentially added.
3° TE: 10 mM TRIS HCl, 1 mM EDTA, pH 8.0.
4° YEME: Difco yeast extract (0.3%), Difco peptone (0.5%), oxoid malt extract (0.3%), glucose (1%).
Transformation of S. lividans Protoplasts
- 1. A culture composed of 25 ml YEME, 34% sucrose, 0.005 M MgCl2, 0.5% glycine, in a 250 ml baffled flask, is centrifuged during 30 to 36 hours.
- 2. The pellet is suspended in 10.3% sucrose and centrifuged. This washing is repeated once.
- 3. The mycelium is suspended in 4 ml lysozyme solution (1 mg/ml in P medium with CaCl2 and MgCl2 concentrations reduced to 0.0025 M) and incubated at 30° C. for 15 to 60 minutes.
- 4. The solution is mixed by pipetting three times in a 5 ml pipette and incubated for further 15 minutes.
- 5. P medium (5 ml) is added and mixed by pipetting as in step 4.
- 6. The solution is filtered through cotton wool and protoplasts are gently sedimented in a bench centrifuge at 800×G during 7 minutes.
- 7. Protoplasts are suspended in 4 ml P medium and centrifuged again.
- 8. Step 7 is repeated and protoplasts are suspended in the drop of P medium left after pouring off the supernatant (for transformation).
- 9. DNA is added in less than 20 μl TE.
- 10. 0.5
ml PEG 1 000 solution (2.5 g PEG dissolved in 7.5 ml of 2.5% sucrose, 0.0014 K2SO4, 0.1 M CaCl2, 0.05 M TRIS-maleic acid, pH 8.0, plus trace elements) is immediately added and pipetted once to mix the components. - 11. After 60 seconds, 5 ml of P medium are added and the protoplasts are sedimented by gentle centrifugation.
- 12. The pellet is suspended in P medium (1 ml).
- 13. 0.1 ml is plated out on R2YE plates (for transformation dry plates to 85% of their fresh weigh e.g. in a laminar flow cabinet).
- 14. Incubation at 30° C.
A—Construction of a “sfr” Gene Cassette
A “sfr” gene cassette was constructed to allow subsequent cloning in plant expression vectors.
Isolation of a FokI-BglII fragment from the plasmid pBG39 containing a “sfr” gene fragment led to the loss of the first codons, including the initiation codon, and of the last codons, including the stop codon.
This fragment of the “sfr” gene could be reconstructed in vitro with synthetic oligonucleotides which encode appropriate amino-acids.
The complementary synthetic oligonucleotides were (SEQ ID NOS:5-6) 5′-CATGAGCCCAGAAC and 3′-TCGGGTCTTGCTGC.
By using such synthetic oligonucleotides, the 5′ end of the “sfr” gene could be reformed and the GTG initiation codon substituted for a codon well translated by plant cells, particularly an ATG codon.
The DNA fragment containing the oligonucleotides linked to the “sfr” gene was then inserted into an appropriate plasmid, which contained a determined nucleotide sequence thereafter designated by an “adapter” fragment.
This adapter fragment comprised:
a TGA termination codon which enabled the last codons of the “sfr” gene to be reformed;
appropriate restriction sites which enabled the insertion of the fragment of the nucleotide sequence comprising the “sfr” gene partially reformed with the synthetic oligonucleotides; this insertion resulted in the reconstruction of an intact “sfr” gene;
appropriate restriction sites for the isolation of the entire “sfr” gene.
The “sfr” gene was then inserted into another plasmid, which contained a suitable plant promoter sequence. The plant promoter sequence consisted of the cauliflower mosaic virus promoter sequence (p35S). Of course the invention is not limited to the use of this particular promoter. Other sequences could be chosen as promoters suitable in plants, for example the TR 1′-2′ promoter region and the promoter fragment of a Rubisco small subunit gene from Argbidopsis thaliana hereafter described.
1° Construction of the Plasmid pLK56.2 (FIG. 3 )
The construction of plasmid pLK56.2 aimed at obtaining a suitable adaptor including the following sequence of restriction sites: SmaI, BamHI, NcoI, KpnI, BglII, MluI, BamHI, HindIII and XbaI.
The starting plasmids used for this construction, pLK56, pJB64 and pLK33 were those disclosed by BOTTERMAN (ref. 11).
The DNA fragments hereafter described were isolated and separated from low melting point agarose (LGA).
The plasmid pLK56 was cleaved by the enzymes BamHI and NdeI. A NcoI-NdeI fragment (referred to in the drawings by arc “a” in broken line) obtained from plasmid pJB64 was substituted in pLK56 for the BamHI-NdeI fragment shown at “b”. Ligation was possible after filling in the BamHI and NcoI protruding ends with the DNA polymerase I of E. coli (Klenow's fragment).
Particularly recircularization took place by means of a T4 DNA ligase. A new plasmid pLK56.3 was obtained.
This plasmid was cleaved by the enzymes XbaI and PstI.
The BamHI-PstI fragment of pLK33 (c) (on FIG. 3 ) was substituted for the XbaI-PstI fragment (d) of pLK56.3, after repairing of their respective ends by Klenow's fragment.
After recircularization by means of the T4 DNA ligase, the obtained plasmid pLK56.2 contained a nucleotide sequence which comprised the necessary restriction sites for the subsequent insertion of the “sfr” gene.
2° Construction of the Plasmid pGSH150 (FIG. 4A )
Parallel with the last discussed construction, there was produced a plasmid containing a promoter sequence recognized by the polymerases of plant cells and including suitable restriction sites, downstream of said promoter sequence in the direction of transcription, which restriction sites are then intented to enable the accomodation of the “sfr” gene then obtainable from pLK56.2, under the control of said plant promoter.
Plasmid pGV825 is described in DEBLAERE et al. (ref. 10). Plasmid pJB63 is from BOTTERMAN (ref. 11).
pGV825 was linearized with PvuII and recircularized by the T4 DNA ligase, resulting in the deletion of an internal PvuII fragment shown at (e), (plasmid pGV956).
pGV956 was then cleaved by BamHI and BglII.
The BamHI-HindIII fragment (f) obtained from pJB63 was dephosphorylated with calf intestine phosphatase (CIP) and substituted for the BamHI-BglII fragment of pGV956.
Plasmid pGV1500 was obtained after recircularization by means of T4 DNA ligase.
An EcoRI-HindIII fragment obtained from plasmid pGSH50 was purified. The latter plasmid carried the dual TR 1′-2′ promoter fragment described in VELTEN et al., (ref. 13). This fragment was inserted in pGV1500, digested with HpaI and HindIII and yielded pGSH150.
This plasmid contains the promoter fragment in front of the 3′ end of the T-DNA transcript 7 and a BamHI and ClaI sites for cloning.
3° Construction of the Plasmid pGSJ260 (FIG. 4B )
CP3 is a plasmid derived from pBR322 and which contains the 35S promoter region of cauliflower mosaic virus within a BamHI fragment.
pGSH150 was cut by BamHI and BglII.
The BamHI-BglII fragment (h) of CP3, which contained the nucleotide sequence of p35S promoter, was substituted for the BamHI-BglII fragment (i) in pGSH150 to form plasmid pGSJ250. pGSJ250 was then opened at its BglII restriction site.
A BamHI fragment obtained from mGV2 (ref. 12) was inserted in pGSJ250 at the BglII site to form plasmid pGSJ260.
However prior to inserting the “sfr” gene obtainable from pLK56.2 into plasmid pGSJ260, it was still desirable to further modify the first in order to permit insertion in a more practical manner. Thus pLK56.2 was further modified as discussed below to yield pGSR1.
Starting from plasmid pGSJ260, two plasmid constructions for subsequent transformations of plant cells were made:
a first plasmid permitting the expression of the “sfr” gene in the cytoplasm of plant cells, and
a second plasmid so modified as to achieve transport of the Bialaphos-resistance enzymes to the chloroplasts of plant cells.
First Case: Plasmid Enabling the Expression of the “sfr” Gene in the Cytoplasm of Plant Cells
Cloning of the sfr Gene Cassette in a Plant Expression Vector (pGSR2) (FIG. 5 )
On FIG. 5A (SEQ ID NO:13), the nucleotide sequence of the adapter of pLK56.2 is shown. In particular, the locations of BamHI, NcoI, BglII restriction sites are shown.
This adapter fragment was cleaved by the enzymes NcoI and BglII.
Using synthetic oligonucleotides, the first codons of the “sfr” gene were reformed, particularly the 5′ end of the gene in which a ATG initiation codon was substituted for the initial GTG codon.
This FokI-BglII fragment completed with the synthetic oligonucleotides was then substituted in pLK56.2 for the NcoI-BglII fragment of the adapter. The 3′ end of the gene was thus reformed too, after recircularization with T4 DNA ligased. The plasmid obtained, pGSR1, thus contained the entire “sfr” gene inserted in its adapter.
The plasmid pGSJ260 was then opened by BamHI (FIG. 5C ) and the BamHI fragment obtained from pGSR1, which contained the entire “sfr” gene, was inserted into pGSJ260.
The obtained plasmid, pGSR2 (see FIG. 6A ) contained a pBR322 replicon, a bacterial streptomycin resistance gene (SDM-SP-AD-transferase) and an engineered T-DNA consisting of:
the border fragments of the T-DNA;
a chimeric kanamycin gene which provided a dominant selectable marker in plant cells; and
a chimeric “sfr” gene.
The chimeric “sfr” gene consisting of:
the promoter region of the cauliflower mosaic virus (p35S);
the “sfr” gene cassette as described in FIG. 5 ;
the 3′ untranslated region, including the polyadenylation signal of T-DNA transcript 7.
pGSR2 was introduced into Agrobacterium tumefaciens recipient C58ClRif® (pGV2260) according to the procedure described by DEBLAERE et al. (ref. 10).
This strain was used to introduce the chimeric “sfr” gene in N. tabacum SR1 plants.
Two variant plasmids deriving from pGSR2, namely pGSFR280 and pGSFR281, have been constructed. They differ in the untranslated sequence following the transcription initiation site. In pGSR2, this fragment consists of the following sequence (SEQ ID NO:7):
GAGGACACGCTGAAATCACCAGTCTCGGATCC ATG;
while it consists of (SEQ ID NO:8):
GAGGACACGCTGAAATCACCAGTCTCTC- TACAAATCGATCCATG
in pGSR280 and of (SEQ ID NO:9)
GAGGACACGCTGAAATCACCAGTCTCTC- TACAAATCGATG
in pGSFR281, with an ATG codon being the initiation codon of the “sfr” gene. The “sfr” gene is also fused to the TR1′-2′ promoter in the plasmid pGSH150 (FIG. 4A ) yielding pGSFR160 and pGSFR161 (FIG. 6B ). These plasmids contain slight differences in the pTR2 “sfr” gene configuration: the “sfr” gene is correctly fused to the endogenous gene 2′ ATG in pGSFR161 (for sequences see ref. 13), whereas 4 extra base pairs (ATCC) are present just ahead of the ATG codon in pGSFR160. Otherwise, plasmids p65FR161 and p65FR160 are completely identical.
All plasmids are introduced in Agrobacterium by cointegration in the acceptor plamids pGV2260 yielding the respective plasmids pGSFR1280, pGSFR1281, pGSFR1160 and pGSFR1161.
Second Case: Construction of a Plasmid Containing the “sfr” Gene Downstream of a DNA Sequence Encoding a Transit Peptide and Suitable for Achieving Subsequent Translocation of the “sfr” Gene Expression Product into Plant-Cell-Chloroplasts
In another set of experiments, the nucleotide sequence which contained the “sfr” gene was fused to a DNA sequence encoding a transit peptide so as to enable its transport into chloroplasts.
A fragment of the “sfr” gene was isolated from the adapter fragment above described and fused to a transit peptide. With synthetic oligonucleotides, the entire “sfr” gene was reconstructed and fused to a transit peptide.
The plasmid (plasmid pATS3 mentioned below) which contained the nucleotide sequence encoding the transit peptide comprised also the promoter sequence thereof.
Construction of the Plasmid pGSR4 which Contains the “sfr” Gene Fused to a DNA Sequence Encoding Transit Peptide (FIG. 7 )
Plasmid pLK57 is from BOTTERMAN, (ref. 11). Plasmid pATS3 is a pUC19 clone which contains a 2 Kb EcoRI genomic DNA fragment from Arabidopsis thaliana comprising the promoter region and the transit peptide nucleotide sequence of the gene, the expression thereof is the small subunit of ribulose biphosphate carboxylase (ssu). The A. thaliana small subunit was isolated as a 1 500 bp EcoRI-SphI fragment. The SphI cleavage site exactly occurs at the site where the coding region of the mature ssu protein starts.
Plasmids pLK57 and pATS3 were opened with EcoRI and SphI. After recircularization by means of the T4 DNA ligase, a recombinant plasmid pLKAB1 containing the sequence encoding the transit peptide (Tp) and its promoter region (Pssu) was obtained.
In order to correctly fuse the “sfr” gene at the cleavage site of the signal peptide, the N-terminal gene sequence was first modified. Since it was observed that N-terminal gene fusions with the “sfr” gene retain their enzymatic activity, the second codon (AGC) was modified to a GAC, yielding an NcoI site overlapping with the ATG initiator site. A new plasmid, pGSSFR2 was obtained. It only differs from pGSR1 (FIG. 5B ), by that mutation. The NcoI-BamHI fragment obtained from pGSFR2 was fused at the SphI end of the transit peptide sequence. In parallel, the “sfr” gene fragment was fused correctly to the ATG initiator of the ssu gene (not shown in figures).
Introduction of the “sfr” Gene into a Different Plant Species
The Bialaphos-resistance induced in plants by the expression of chimeric genes, when the latter have been transformed with appropriate vectors containing said chimeric genes, has been demonstrated as follows. The recombinant plasmids containing the “sfr” gene were introduced separately by mobilization into Agrobacterium strain C58C1 Rif® (pGV2260) according to the procedure described by DEBLAERE and al., Nucl. Acid. Res., 13, p. 1 477, 1985. Recombinant strains containing hybrid Ti plasmides were formed. These strains were used to infect and transform leaf discs of different plant species, according to a method essentially as described by HORSH and al., 1985, Science, vol. 227. Transformation procedure of these different plant species given by way of example, is described thereafter.
- 1. Leaf Disc Transformation of Nicotiana tabacum
Used Media are described thereafter:
A1 MS salt/2 | +1% | sucrose | |
0.8% | agar | ||
pH 5.7 | |||
A10 B5-medium | +250 | mg/l NH4NO3 | |
750 | mg/l CaCl2 2H2O | ||
0.5 | g/l 2-(N-Morpholino)ethane- | ||
sulfonic acid (MES) pH 5.7 | |||
30 | g/l sucrose | ||
A11 B5-medium | +250 | mg/l NH4NO3 | |
0.5 | g/l MES pH 5.7 | ||
2% | glucose | ||
0.8 | agar | ||
40 | mg/l adenine | ||
+1 | mg/l 6-Benzylaminopurine | ||
(BAP) | |||
0.1 | mg/l Indole-3-acetic acid | ||
(IAA) | |||
500 | mg/l Claforan | ||
A12 B5-medium | +250 | mg/l NH4NO3 | |
0.5 | g/l MES pH 5.7 | ||
2% | glucose | ||
0.8 | agar | ||
40 | mg/l adenine | ||
+1 | mg/ |
||
200 | mg/l claforan | ||
A13 MS-salt/2 | +3% | sucrose | |
0.5 | MES g/l pH 5.7 | ||
0.7 | agar | ||
200 | mg/l claforan | ||
Bacterial medium = min A: | (Miller 1972) 60 mM | ||
K2HPO4, 3H2O, | |||
33 mM KH2PO4; 7.5 mM (NH4)2 | |||
SO4 1.7M trinatriumcitrat; 1 mM | |||
MgSO4; | |||
2 g/l glucose; 50 mg/l vitamine B1 | |||
Plant Material:
Nicotiana tabacum cv. Petit Havana SR1
Plants are used 6 to 8 weeks after subculture on medium A1
Infection:
midribs and edges are removed from leaves.
Remaining parts are cut into segments of about 0.25 cm2 and are placed in the infection medium A10 (about 12 segments in a 9 cm Petri dish containing 10 ml A10).
Segments are then infected with 25 μl per Petri dish of a late log culture of the Agrobacterium strain grown in min A medium.
Petri dish are incubated for 2 to 3 days at low light intensity.
After 2 to 3 days medium is removed and replaced by 20 ml of medium A10 containing 500 mg/l clarofan.
Selection and Shoot Induction
The leaf discs are placed on medium A11 containing a selective agent:
100 mg/l kanamycin and
10 to 100 mg/l phosphinotricin.
Leaf discs are transferred to fresh medium weekly.
After 3 to 4 weeks regenating calli arise. They are separated and placed on medium A12 with the same concentration of selective agent as used for the selection.
Rooting
After 2 to 3 weeks the calli are covered with shoots, which can be isolated and transferred to rooting medium A13 (without selection).
Rooting takes 1 to 2 weeks.
After a few more weeks, these plants are propagated on medium A1.
- 2. Tuber Disc Infection of Solanum tuberosum (Potato)
Used media are described thereafter:
C1 B5-medium | +250 | mg/l NH4NO3 |
300 | mg/l (CaCH2PO4)2 | |
0.5 | g/l MES pH 5.7 | |
0.5 | g/l polyvinylpyrrolidone (PVP) | |
40 | g/l mannitol (=0.22M) | |
0.8% | agar | |
40 | mg/l adenine | |
C2 B5-medium | +250 | mg/l NH4NO3 |
400 | mg/l glutamine | |
0.5 | g/l MES pH 5.7 | |
0.5 | g/l PVP | |
40 | g/l mannitol | |
40 | mg/l adenine | |
0.8% | agar | |
+0.5 | mg/l transzeatine | |
0.1 | mg/l IAA | |
500 | mg/l clarofan | |
C5 MS salt/2 | +3% | sucrose |
0.7% | agar | |
pH 5.7 | ||
C7 B5-medium | +250 | mg/l NH4NO3 |
400 | mg/l glutamine | |
0.5 | g/l MES pH 5.7 | |
0.5 | g/l PVP | |
20 | g/l mannitol | |
20 | g/l glucose | |
40 | mg/l adenine | |
0.6% | agarose | |
+0.5 | mg/l transzeatine | |
0.1 | mg/l IAA | |
500 | mg/l clarofan | |
C8 B5-medium | +250 | mg/l NH4NO3 |
400 | mg/l glutamine | |
0.5 | g/l MES pH 5.7 | |
0.5 | g/l PVP | |
20 | g/l mannitol | |
20 | g/l glucose | |
40 | mg/l adenine | |
0.6% | agarose | |
+200 | mg/l clarofan | |
1 | mg/l transzeatine | |
C9 B5-medium | +250 | mg/l NH4NO3 |
400 | mg/l glutamine | |
0.5 | g/l MES pH 5.7 | |
0.5 | g/l PVP | |
20 | g/l mannitol | |
20 | g/l glucose | |
40 | mg/l adenine | |
0.6% | agarose | |
+1 | mg/l transzeatine | |
0.01 | mg/l Gibberellic acid A3 | |
(GA3) | ||
100 | mg/l clarofan | |
C11 MS salt/2 | +6% | sucrose |
0.7% | agar | |
Bacterial medium = min | (Miller 1972 60 mM K2HPO4•3H2O; | |
A: | 33 mM KH2PO4; 7.5 mM (NH4)2SO4; | |
1.7 trinatriumcitrat; 1 mM | ||
MgSO4; | ||
2 g/l glucose; 50 mg/l vitamine B1. | ||
Plant Material
Tubers of Solanum tuberosum c.v Berolina
c.v Désirée
Infection
Potatoes are peeled and washed with water.
Then they are washed with concentrated commercial bleach for 20 minutes, and
rinsed 3 to 5 times with sterile water.
The outer layer is removed (1 to 1.5 cm)
The central part is cut into discs of about 1 cm2 and 2 to 3 mm thick.
Discs are placed on medium C1 (4 pieces in a 9 cm Petri dish).
10 μl of a late log culture of an Agrobacterium strain grown in min A medium is applied on each disc.
Discs are incubated for 2 days at low light intensity.
Selection and Shoot Induction
Discs are dried on a filter paper and transferred to medium C2 with 100 mg/l kanamycin.
After one month small calli are removed from the discs and transferred to medium C7 containing 50 mg/l kanamycin.
After a few more weeks, the calli are transferred to medium C8 containing 50 mg/l kanamycin.
If little shoots start to develop, the calli are transferred to elongation medium C9 containing 50 mg/l Kanamycin.
Rooting
Elongated shoots are separated and transferred to rooting medium C11.
Rooted shoots are propagated on medium C5.
- 3. Leaf Disc Infection of Lycopersicum esculentum (Tomato)
Used media are described thereafter
A1 MS salt/2 | +1% | sucrose |
0.8% | agar | |
pH 5.7 | ||
B1 B5-medium | +250 | mg/l NH4NO3 |
0.5 | g/l MES pH 5.7 | |
0.5 | g/l PVP | |
300 | mg/l Ca (H2PO4)2 | |
2% | glucose | |
40 | mg/l adenine | |
40 | g/l mannitol | |
B2 B5-medium | +250 | mg/l NH4NO3 |
0.5 | g/l MES pH 5.7 | |
0.5 | g/l PVP | |
400 | mg/l glutamine | |
2% | glucose | |
0.6% | agarose | |
40 | mg/l adenine | |
40 | g/l mannitol | |
+0.5 | mg/l transzeatine | |
0.01 | mg/l IAA | |
500 | mg/l claforan | |
B3 B5-medium | +250 | mg/l NH4NO3 |
0.5 | g/l MES pH 5.7 | |
0.5 | g/l PVP | |
400 | mg/l glutamine | |
2% | glucose | |
0.6% | agarose | |
40 | mg/l adenine | |
30 | g/l mannitol | |
+0.5 | mg/l transzeatine | |
0.01 | mg/l IAA | |
500 | mg/l clarofan | |
B4 B5-medium | +250 | mg/l NH4NO3 |
0.5 | g/l MES pH 5.7 | |
0.5 | g/l PVP | |
400 | mg/l glutamine | |
2% | glucose | |
0.6% | agarose | |
40 | mg/l adenine | |
20 | g/l mannitol | |
+0.5 | mg/l transzeatine | |
0.01 | mg/l IAA | |
500 | mg/l clarofan | |
B5 B5-medium | +250 | mg/l NH4NO3 |
0.5 | g/l MES pH 5.7 | |
0.5 | g/l PVP | |
400 | mg/l glutamine | |
2% | glucose | |
0.6% | agarose | |
40 | mg/l adenine | |
10 | g/l mannitol | |
+0.5 | mg/l transzeatine | |
0.01 | mg/l IAA | |
500 | mg/l clarofan | |
B6 B5-medium | +250 | mg/l NH4NO3 |
0.5 | g/l MES pH 5.7 | |
0.5 | g/l PVP | |
400 | mg/l glutamine | |
2% | glucose | |
0.6% | agarose | |
40 | mg/l adenine | |
+0.5 | mg/l transzeatine | |
0.01 | mg/l IAA | |
200 | mg/l clarofan | |
B7 B5-medium | +250 | mg/l NH4NO3 |
0.5 | g/l MES pH 5.7 | |
0.5 | g/l PVP | |
400 | mg/l glutamine | |
2% | glucose | |
0.6% | agarose | |
40 | mg/l adenine | |
+1 | mg/l transzeatine | |
200 | mg/l clarofan | |
B8 MS salt/2 | +2% | sucrose |
0.5 | g/l MES pH 5.7 | |
0.7% | agar | |
B9 B5-medium | +250 | mg/l NH4NO3 |
0.5 | g/l MES pH 5.7 | |
0.5 | g/l PVP | |
2% | glucose | |
0.6% | agarose | |
40 | mg/l adenine | |
+1 | mg/l transzeatine | |
0.01 | mg/l GA3 | |
Bacterial medium = min | (Miller 1972) 60 mM | |
A: | K2HPO4•3H2O; | |
33 mM KH2PO4; 7.5 mM (NH4)2SO4; | ||
1.7M trinatriumcitrat; 1 mM MgSO4; | ||
2 g/l glucose; 50 mg/l vitamin B1 | ||
Plant Material
Lycopersicum esculentum cv. Lucullus.
Plants are used 6 weeks after subculture on medium A1.
Infection
Midrib is removed from the leaves.
Leaves are cut in segments of about 0.25 to 1 cm2 (the edges of the leaves are not wounded, so that only maximum 3 sides of the leaf pieces is wounded).
Segments are placed in infection medium B1 (upside down), about 10 segments in a 9 cm Petri dish.
Segments are then infected wiht 20 μl per Petri dish of a late log culture of the Agrobacterium strain grown in min A medium.
Petri dishes incubate for 2 days at low light intensity.
Medium is removed after 2 days and replaced by 20 ml of medium B1 containing 500 mg/l clarofan.
Selection and Shoot Induction
The leaf discs are placed in medium B2+50 or 100 mg/l kanamycin.
Each 5 days the osmotic pressure of the medium is lowered by decreasing the mannitol concentration, transfers are done consecutively in medium B3, B4, B5, and B6.
After one month calli with meristems are separated from the leaf discs and placed on medium B7 with 50 or 100 mg/l kanamycin.
Once little shoots have formed, calli are transferred to elongation medium B9 with 50 or 100 mg/l kanamycin.
Rooting
Elongated shoots are separated and transferred to medium B8 for rooting.
Plants are propagated on medium A1.
Greenhouse Tests for Herbicide Resistance
Material and Method
In this experiment, two herbicides comprising phosphinotricin as active ingredient, are used.
These compounds are those commercially available under the registered trademarks BASTA® and MEIJI HERBIACE®.
These products are diluted to 2% with tap water. Spraying is carried out on a square meter area from the four corners. Temperature of the greenhouse is about 22° C. for tobaccos and tomato, and above 10° C. to 15° C. for potato.
Results
Tobacco Spraytest
a) Nicotiana tabacum cv. Petit Havana SR1 plants transformed with the chimeric “sfr” genes as present in pGSFR1161 or pGSFR1281, as well as unstransformed control plants (from 10 cm to 50 cm high) are treated with 20 1 BASTA®/ha. Control SR1 plants die after 6 days, while transformed plants are fully resistant to 20 1 BASTA®/ha and continue growing undistinguishable from untreated plants. No visible damage is detected, also the treatment is repeated every two weeks. The treatment has no effect in subsequent flowering. The recommended dose oF BASTA® herbicide in agriculture is 2.5-7.5 l/ha.
b) A similar experiment is performed using 8 l/ha MEIJI HERBIACE®. The transformed plants (the same as above) are fully resistant and continue growing undistinguishable from untreated plants. No visible damage is detectable.
Potato Spraytest
Untransformed and transformed potato plants (Solanum tuberosum cv. Berolina) (20 cm high) with the chimeric “sfr” gene as present in pGSFR1161 or pGSFR1281 are treated with 20 1 BASTA®/ha. Control plants die after 6 days while the transformed plants do not show any visible damage. They grow undistiguishable from untreated plants.
Tomato Spraytest
Untransformed and transformed tomato plants (lycopersium esculentum c.v. luculus) (25 cm high) with the chimeric “sfr” gene as present in pGSFR1161 and pGSFR1281 are treated with 20 1 BASTA®/ha. control plants die after six days while transformed plants are fully resistant. They do not show any visible damage and grow undistiguishable from untreated plants.
Growth Control of Phytopathogenic Fungi with Transformed Plants
In another set of experiments, potato plants expressing chimeric “sfr” genes as present in pGSFR1161 or pGSFR1281 are grown in a greenhouse compartment at 20° C. under high humidity. Plants are innoculated by spraying 1 ml of a suspension of 106 Phytophtora infestans spores per ml. Plants grow in growth chambers (20° C., 95% humidity, 4 000 lux) until fungal disease symptoms are visible (one week). One set of the plants are at that moment sprayed with Bialaphos at a dose of 8 l/ha. Two weeks later, untreated plants are completely ingested by the fungus. The growth of the fungus is stopped on the Bialaphos treated plants and no further disease symptoms evolve. The plants are effectively protected by the fungicide action of Bialaphos.
Transmission of the PPT Resistance through Seeds
Transformed tobacco plants expressing the chimeric “sfr” gene present in pGSFR1161 and pGSFR1281 are brought to flowering in the greenhouse. They show a normal fertility.
About 500 F1 seeds of each plant are sown in soil, F1 designating seeds of the first generation, i.e directly issued from the originally transformed plants. When seedlings are 2-3 cm high, they are sprayed with 8 1 BASTA®/ha. 7 days later, healthy and damaged plants can be distinguished in a ratio of approximately 3 to 1. this shows that PPT resistance is inherited as a dominant marker encoded by a single locus.
10 resistant F1 seedlings are grown to maturity and seeds are harvested. F2 seedlings are grown as described above and tested for PPT-resistance by spraying BASTA® at a dose of 8 l/ha. Some of the F1 plants produce F2 seedlings which are all PPT-resistant showing that these plants are homozygous for the resistance gene. The 5 invention also concerns plant cells and plants non-essentially-biologically-transformed with a GS inhibitor-inactivating-gene according to the invention.
In a preferred embodiment of the invention, plant cells and plants are non-biologically-transformed with the “sfr” gene hereabove described.
Such plant cells and plants possess, stably integrated in their genome, a non-variety-specific character which render them able to produce detectable amounts of phosphinotricinacetyl transferase.
This character confers to the transformed plant cells and plants a non-variety-specific enzymatic activity capable of inactivating or neutralizing GS inhibitors like Bialaphos and PPT.
Accordingly, plant cells and plants transformed according to the invention are rendered resistant against the herbicidal effects of Bialaphos and related compounds.
Since Bialaphos was first described as a fungicide, transformed plants can also be protected against fungal diseases by spraying with the compound several times.
In a preferred embodiment, Bialaphos or related compounds is applied several times, particularly at time intervals of about 20 to 100 days.
The invention also concerns a new process for selectively protecting a plant species against fungal diseases and selectively destroying weeds in a field comprising the steps of treating a field with an herbicide, wherein the plant species contain in their genome a DNA fragment encoding a protein having an enzymatic activity capable of neutralizing or inactivating GS inhibitors and wherein the used herbicide comprises as active ingredient a GS inhibitor.
It comes without saying that the process according to the invention can be employed with the same efficiency, either to only destroy weeds in a field, if plants are not infected with fungi, either to only stop the development of fungi if the latter appears after destruction of weeds.
In a preferred embodiment of the process according to the invention, plant species are transformed with a DNA fragment comprising the “sfr” gene as described hereabove, and the used herbicide is PPT or a related compound.
Accordingly, a solution of PPT or related compound is applied over the field, for example by spraying, several times after emergence of the plant species to be cultivated, until early and late germinating weeds are destroyed.
It is quite evident that before emergence of plant species to be cultivated, the field can be treated with an herbicidal composition to destroy weeds.
On the same hand, fields can be treated even before the plant species to be cultivated are sowed.
Before emergence of the desired plant species, fields can be treated with any available herbicide, including Bialaphos-type herbicides.
After emergence of the desired plant species, Bialaphos or related compound is applied several times.
In a preferred embodiment, the herbicide is applied at time intervals of about from 20 to 100 days.
Since plants to be cultivated are transformed in such a way as to resist to the herbicidal effects of Bialaphos-type herbicides, fields can be treated even after emergence of the cultivated plants.
This is particularly useful to totally destroy early and late germinating weeds, without any effect on the plants to be produced.
Preferably, Bialaphos or related compoud is applied at a dose ranging from about 0.4 to about 1.6 kg/ha, and diluted in a liquid carrier at a concentration such as to enable its application to the field at a rate ranging from about 2 to about 8 l/ha.
There follows examples, given by way of illustration, of some embodiments of the process with different plant species.
Sugarbeets
The North European sugarbeet is planted from March 15 up to April 15, depending upon the weather condition and more precisely on the precipitation and average temperature. the weeds problems are more or less the same in each country and can cause difficulties until the crop closes its canopy around mid-July.
Weed problems can be separated in three situations:
-
- early germination of the grassy weeds,
- early germinating broadleaved weeds,
- late germinating broadleaved weeds.
Up to now, pre-emergence herbicides have been succesfully used. Such compounds are for example those commercially available under the registered trademarks: PYRAMIN®, GOLTIX® and VENZAR®. However, the susceptibility to dry weather conditions of these products as well as the lack of residual activity to control late germinating weeds have led the farmer to use post-emergence products in addition to pre-emergence ones.
Table (I) thereafter indicates the active ingredients contained in the herbicidal compositions cited in the following examples.
TABLE I | ||||
Commercial Name | Active Ingredient | Formulation | ||
AVADEXR | Diallate | EC 400 g/l | ||
AVADEX BWR | Triallate | EC 400 g/l | ||
GOLTIXR | Metamitron | WP 70% | ||
RONEETR | Cycloate | EC 718 g/l | ||
TRAMATR | Ethofumerate | EC 200 g/l | ||
FERVINALR | Alloxydime-sodium | SP 75% | ||
BASTAR | Phosphinotricin | 200 g/l | ||
PYRAMIN FLR | Chloridazon | SC 430 g/l | ||
According to the invention, post-emergence herbicides consist of Bialaphos or related compounds, which offer a good level of growth control of annual grasses (Bromus, Avena spp., Alopecurus, POA) and broadleaves (Galium, Polygonum, Senecio, Solanum, Mercurialis).
Post-emergence herbicides can be applied at different moments of the growth of sugarbeet; at a cotyledon level, two-leave level or at a four-leave level.
Table (II) thereafter represents possible systems of field-treatment, given by way of example.
In those examples, the post-emergence herbicide of the class of Bialaphos used is BASTA®, in combination with different pre-emergence herbicides. Concentrations are indicated in l/ha or kg/ha.
TABLE II |
POSSIBLE WEEDCONTROL SYSTEMS IN SUGARBEETS, BASED ON THE USE OF |
BASTAR, PROVIDING BEETS ARE MADE RESISTANT AGAINST THE LATTER |
CHEMICAL (in lt or kg/ha). |
Pre-sowing | Pre-emergence | Cotyledons | Two-leaves | Four leaves | |
1. | AVADEXR | — | BASTAR | BASTAR/tramat | — |
3.5 |
3 |
3 lt 1.5 lt | — | ||
2. | AVADEXR | GOLTIXR | — | — | — |
3.5 lt | 4 |
||||
3 | RONEETR | GOLTIXR | — | — | — |
4 |
5 kg | ||||
4. | RONEETR | GOLTIXR | — | BASTAR | — |
4 lt | 2.5 |
3 |
|||
5. | TRAMATR | — | — | BASTAR | BASTAR/ |
5 |
3 |
2 |
|||
6. | — | GOLTIXR | — | BASTAR | — |
2.5 |
3 lt | ||||
7. | — | — | BASTAR/tramat | — | BASTAR/ |
3 lt 1.7 |
3 |
||||
8. | PYRAMINR | — | BASTAR | Venzar | — |
6 |
3 |
1 kg | |||
9. | — | — | BASTAR | BASTAR/GOLTIXR | — |
3 |
3 |
||||
10. | DIALLATER | PYRAMINR | BASTAR/Metamitron | — | |
3.5 |
6 |
3 |
|||
Potatoes
Potatoes are grown in Europe on about 8.106 Ha. The major products used for weed control are Linuron/mono-linuron or the compound commercially available under the denomination METRABUZIN
These products perform well against most weedspecies.
However, weeds such as Galium and Solanum plus late germinating Chenopodium and Polygonum are not always effectively controlled, while control of the annual grasses is also sometime erratic.
Once again, late germinating broadleaved weeds are only controllable by post-emergence applications of herbicides such as BASTA®.
Table (III) thereafter represents some examples given by way of example of field-treatment in the case of potatoes.
TABLE III |
Weeds control systems in potatoes, based on the use of BASTR, |
providing potatoes are rendered resistant to BASTAR. |
Linuron + monolinuron | (375 g + 375 g/ha) prior to emergence | |
BASTAR | 3-4 lt/ha after emergence (5-15 cm) | |
BASTAR/fluazifop-butyl | 3-4 lt/ha + 2 lt/ha after emergence (5-15 cm) | |
| WP | 50% (AFALONR) |
Monolinuron | WP 47.5% (ARESSINR) | |
fluazifop-butyl | EL 250 g/l (FUSILADER) | |
The strains PGSJ260 and pBG39 used hereabove have been deposited on Dec. 12, 1985, at the “German Collection of Micro-organisms” (DEUTSCHE SAMMLUNG VON MIKROORGANISMEN) at Gottingen, Germany. They received the deposition numbers DSM 3 606 and DSM 3 607 respectively.
Further embodiments of the invention are described hereafter with reference to the figures in which:
Another Bialaphos-resistance-gene has been isolated form another Bialaphos-producing-strains, i.e. streptomyces viridochromogenes. This second resistance-gene is thereafter designated by “sfrsv” gene.
This second preferred DNA fragment according to the invention, for the subsequent transformation of plant cells, consists of a nucleotide sequence (SEQ ID NO:11) coding for at least part of a polypeptide having the following sequence:
V S P E R R P V E I R P A T A A D M | |
A A V C D I V N H Y I E T S T V N P | |
R T E P Q T P Q E W I D D L E R L Q | |
D R Y P W L V A E V E G V V A G I A | |
Y A G P W K A R N A Y D W T V E S T | |
V Y V S H R H Q R L G L G S T L Y T | |
H L L K S M E A Q G F K S V V A V I | |
G L P N D P S V R L H E A L G Y T A | |
R G T L R A A G Y K H G G W H D V G | |
F W Q R D F E L P A P P R P V R P V | |
T Q I * |
which part of said polypeptide is of sufficient length to confer protection against Bialaphos-“plant-protecting-capability”-, to plant cells, when incorporated genetically and expressed therein. Reference will also be made hereafter to the “plant-protecting-capability”against Bialaphos of the abovesaid nucleotide sequence.
Meaning of the designation of amino acids by a single letter is given therafter.
Alanine | A | Leucine | L | ||
Arginine | R | Lysine | K | ||
Asparagine | N | Methionine | M | ||
Aspartic Acid | D | Phenylalanine | F | ||
Cysteine | C | Proline | P | ||
Cystine | C | Serine | S | ||
Glycine | G | Threoriine | T | ||
Glutamic Acid | E | Tryptophan | W | ||
Glutamine | Q | Tyrosine | Y | ||
Histidine | H | Valine | V | ||
Isoleucine | I | ||||
This second preferred DNA fragment consists of the following nucleotide sequence (SEQ ID NO:12):
TAAAGAGGTGCCCGCCACCCGCTTTCGCAGAACACCGAAGGAGACCACAC |
↓ |
GTGAGCCCAGAACGACGCCCGGTCGAGATCCGTCCCGCCACCGCCGCCGA |
CATGGCGGCGGTCTGCGACATCGTCAATCACTACATCGAGACGAGCACGG |
TCAACTTCCGTACGGAGCCGCAGACTCCGCAGGAGTGGATCGACGACCTG |
GAGCGCCTCCAGGACCGCTACCCCTGGCTCGTCGCCGAGGTGGAGGGCGT |
CGTCGCCGGCATCGCCTACGCCGGCCCCTGGAAGGCCCGCAACGCCTACG |
ACTGGACCGTCGAGTCGACGGTGTACGTCTCCCACCGGCACCAGCGGCTC |
GGACTGGGCTCCACCCTCTACACCCACCTGCTGAAGTCCATGGAGGCCCA |
GGGCTTCAAGAGCGTGGTCGCCGTCATCGGACTGCCCAACGACCCGAGCG |
TGCGCCTGCACGAGGCGCTCGGATACACCGCGCGCGGGACGCTGCGGGCA |
GCCGGCTACAAGCACGGGGGCTGGCACGACGTGGGGTTCTGGCAGCGCGA |
CTTCGAGCTGCCGGCCCCGCCCCGCCCCGTCCGGCCCGTCACACAGATCT |
↑ |
GAGCGGAGAGCGCATGGC |
or of a part thereof expressing a polypeptide having plant-protecting capability against Bialaphos;
There follows hereafter the description of experiments carried out for the isolation of the “sfrsv” resistance gene, the construction of expression vectors which contain the resistance gene and which allow the subsequent transformation of plant cells, in order to render them resistant to GS inhibitors.
Cloning of the Bialaphos-Resistance-“sfrsv” Gene from Streptomyces viridochromogenes
The strain Streptomyces viridochromogenes DSM 40736 (ref 1) was grown and total DNA of this strain was prepared according to standard techniques. DNA samples were digested respectively with PstI, SmaI and Sau3AI in three different reactions and separated on an agarose gel, together with plasmid DNA from pGSR1 (FIG. 5B ) digested with BamHI. In a Southern analysis the DNA was blotted on a nitrocellulose filter and hybridized with the labbeled BamHI fragment from pGSR1 containing the “sfr” gene. In all four lanes of the gel, a restriction fragment was showing strong homology with the probe: a PstI fragment of about 3 kb, a SmaI fragment of about 1.2 kb and Sau3AI fragment of 0.5 kb. In order to clone this gene, PstI restriction fragments were directly cloned in the Escherichia coli vector pUC8. 3000 colonies obtained after transformation were transferred to nitrocellulose filters, and hybridized with the “sfr” probe. Positive candidates were further tested for their growth on minimal medium plates containing 300 μg/ml PPT. One transformant that grew on PPT-containing-medium was further analysed. The plasmid map and relevant restriction sites of this plasmid pJS1 are represented in FIG. 8 . The strain MC1061 (pJS1) has been deposited on Mar. 6, 1987 at the DEUTSCHE SAMMLUNG VON MIKROORGANISMEN (DSM) under deposition number DSM 4023. The clone restriction fragment has been sequenced according to the Maxam and Gilbert method and the coding region of the gene could be identified through homology. The sequence of the “sfrsv” gene is represented in FIG. 9 and the homology on the nucleotide and amino acid sequence level with “sfr” gene is shown in FIG. 10 .
Expression of the “sfrsv” Gene
A “sfrsv gene cassette” was also constructed to allow subsequent cloning in plant expression vectors. A BanII-BglII fragment containing the “sfrsv” coding region without the initiation codon GTG was isolated from PJS1. This fragment was ligated in the vector pLK56-2 digested with NcoI and BglII, together with a synthetic oligonucleotide 5′-CATGAGCC-3′, similar with the one described for “sfr” gene and shown in FIG. 5 . The construction of pGSR1SV is schematically shown in FIG. 11 . Since similar cassettes of both genes are present in respectively pGSR1 and pGSR1SV, previous described constructions for the expression of the “sfr” gene in plants can be repeated.
Enzymatic analysis of crude extracts from E. coli strains carrying plasmid pGSR1SV demonstrated the synthesis of an acetylase which could acetylate PPT. This was shown by thin layer chromotography of the reaction porducts.
The “sfrsv” gene was then inserted into the plasmid vector pGSJ260 (FIG. 4B ) under the control of the CaMV 35s promoter, to yield a plasmid pGS2SV, similar to pGSR2 (FIG. 6A ) except that the “sfrsv” gene is substituted for the “sfr” gene.
It is clear that herbicide resistance genes of the above type may be obtained from many other microorganisms that produce PPT or PPT derivatives. Herbicide resistance gene can then be incorporated in plant cells with a view of protecting them against the action of such Glutamine Synthetase inhibitors. For instance, a Bialaphos-resistance-gene is obtained from Kitasotosporia (ref. 15).
Transformed plant cells and plants which contain the “sfrsv” resistance gene can be obtained with plasmid pGSR2SV, using the same Agrobacterium-mediated-transformation system as hereabove described for the transformation of different plant species with the “sfr” gene.
Plants are regenerated and tested for their resistance with similar spraying tests as described hereabove. All plants behaved similarly and show resistance against herbicides consisting of glutamine synthetase inhibitors.
Finally, the inventors also pertains to the combination of the plants resistant to an inhibitor of glutamine synthetase as defined above with the corresponding inhibitor of glutamine synthetase for use in the production of cultures of said plants free form weeds.
- 1. BAYER et al., HELVETICA CHEMICA ACTA, 1972
- 2. WAKABAYASHI K. and MATSUNAKA S., Proc. 1982, British Crop Protection Conference, 439-450
- 3. M. MASON et al., PHYTOCHEMISTRY, 1982, vol. 21, No. 4, p. 855-857.
- 4. C. J. THOMPSON et al., NATURE, Jul. 31, 1980, vol. 286, No. 5 772, p. 525-527
- 5. C. J. THOMPSON et al., JOURNAL OF BACTERIOLOGY, August 1982, p. 678-685
- 6. C. J. THOMPSON et al.,
GENE 20, 1982, p. 51-62 - 7. C. J. THOMPSON et al., MOL. GEN. GENET., 1984, 195, p. 39-43
- 8. TOWBIN et al., PROC. NATL. ACAD. SCI. USA, 1979, 76, p. 4 350-4 354
- 9. METHODS OF ENZYMOLOGY, V.XLIII, p. 737-755
- 10. DEBLAERE H. et al., 1985, Nucl. Acid. Res., 13, 1 477
- 11. BOTTERMAN J., February 1986, Ph. D. Thesis, State University of Ghent
- 12. DEBLAERE R., February 1986, Ph. D Thesis, Free University of Brussel, Belgium
- 13. VELTEN et al, EMBO J. 1984, vol. 3, No. 12, p. 2 7232-2 730
- 14. CHATER et al, Gene cloning in Streptomyces. Curr. Top. Microbiol. Immunol., 1982, 96, p. 69-75
- 15. OMURA et al, J. of Antibiotics, Vol. 37, 8, 939-940, 1984
- 16. MURAKAMI et al, Mol. Gen. Genet., 205, 42-50, 1986
- 17. MANDERSCHEID and WILD, J. Plant Phys., 123, 135-142, 1986
Claims (2)
1. An isolated DNA encoding a protein having phosphinothricin acetyltransferase activity, or a variant thereof retaining said activity, said protein comprising the amino acid sequence (SEQ ID No. 1):
in which X is Met or Val, said DNA consisting of between 549 and 625 nucleotides wherein X is encoded by ATG.
2. The isolated DNA of claim 1 , consisting of the nucleotide sequence (SEQ ID No. 2 from nucleotide 2 to nucleotide 549):
in which N is A or G.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US14/017,108 USRE44962E1 (en) | 1986-03-11 | 2013-09-03 | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants |
Applications Claiming Priority (9)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP86400521 | 1986-03-11 | ||
EP87400141A EP0242236B2 (en) | 1986-03-11 | 1987-01-21 | Plant cells resistant to glutamine synthetase inhibitors, made by genetic engineering |
GB87400141.5 | 1987-01-21 | ||
GB86400521.0 | 1987-01-21 | ||
PCT/EP1987/000141 WO1987005629A1 (en) | 1986-03-11 | 1987-03-11 | Plant cells resistant to glutamine-synthetase inhibitors, made by genetic engineering |
US13114087A | 1987-11-05 | 1987-11-05 | |
US07/525,300 US5561236A (en) | 1986-03-11 | 1990-05-17 | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants |
US08/465,219 US7112665B1 (en) | 1986-03-11 | 1995-06-05 | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants |
US14/017,108 USRE44962E1 (en) | 1986-03-11 | 2013-09-03 | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants |
Related Parent Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US08/465,219 Reissue US7112665B1 (en) | 1986-03-11 | 1995-06-05 | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants |
Publications (1)
Publication Number | Publication Date |
---|---|
USRE44962E1 true USRE44962E1 (en) | 2014-06-24 |
Family
ID=26105540
Family Applications (5)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US07/525,300 Expired - Lifetime US5561236A (en) | 1986-03-11 | 1990-05-17 | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants |
US08/463,241 Expired - Lifetime US5646024A (en) | 1986-03-11 | 1995-06-05 | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants |
US08/465,219 Ceased US7112665B1 (en) | 1986-03-11 | 1995-06-05 | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants |
US08/477,320 Expired - Lifetime US5648477A (en) | 1986-03-11 | 1995-06-07 | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants |
US14/017,108 Expired - Lifetime USRE44962E1 (en) | 1986-03-11 | 2013-09-03 | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants |
Family Applications Before (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US07/525,300 Expired - Lifetime US5561236A (en) | 1986-03-11 | 1990-05-17 | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants |
US08/463,241 Expired - Lifetime US5646024A (en) | 1986-03-11 | 1995-06-05 | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants |
US08/465,219 Ceased US7112665B1 (en) | 1986-03-11 | 1995-06-05 | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants |
US08/477,320 Expired - Lifetime US5648477A (en) | 1986-03-11 | 1995-06-07 | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants |
Country Status (21)
Country | Link |
---|---|
US (5) | US5561236A (en) |
EP (2) | EP0242236B2 (en) |
JP (2) | JP3142848B2 (en) |
KR (1) | KR880701281A (en) |
AT (2) | ATE57390T1 (en) |
AU (1) | AU612570B2 (en) |
BR (1) | BR8706204A (en) |
CL (1) | CL2004001483A1 (en) |
DE (2) | DE3765449D1 (en) |
DK (2) | DK175656B1 (en) |
ES (2) | ES2018274T5 (en) |
FI (1) | FI874883A0 (en) |
GR (3) | GR3001220T3 (en) |
HK (1) | HK1000519A1 (en) |
HU (1) | HU213580B (en) |
IL (1) | IL81838A (en) |
NO (1) | NO874673D0 (en) |
OA (1) | OA08771A (en) |
PT (1) | PT84448B (en) |
WO (1) | WO1987005629A1 (en) |
ZA (1) | ZA871754B (en) |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9992943B2 (en) | 2016-04-11 | 2018-06-12 | Bayer Cropscience Lp | Cotton variety ST 4949GLT |
US10010041B2 (en) | 2016-04-11 | 2018-07-03 | Bayer Cropscience Lp | Cotton variety ST 4848GLT |
US10448611B2 (en) | 2016-04-11 | 2019-10-22 | Basf Agricultural Solutions Seed, Us Llc | Cotton variety FM 1911GLT |
US10470428B2 (en) | 2018-03-07 | 2019-11-12 | Basf Agricultural Solutions Seed, Us Llc | Cotton variety ST 5471GLTP |
US10827718B2 (en) | 2017-02-13 | 2020-11-10 | Basf Agricultural Solutions Seed, Us Llc | Cotton variety ST 5020GLT |
US10874082B2 (en) | 2017-02-13 | 2020-12-29 | Basf Agricultural Solutions Seed, Us Llc | Cotton variety FM 1953GLTP |
US11076548B2 (en) | 2018-03-07 | 2021-08-03 | Basf Agricultural Solutions Seed, Us Llc | Cotton variety ST 5122GLT |
US11213003B2 (en) | 2019-02-12 | 2022-01-04 | BASF Agricultural Solutions Seed US LLC | Cotton variety ST 4550GLTP |
US11234407B2 (en) | 2019-02-12 | 2022-02-01 | BASF Agricultural Solutions Seed US LLC | Cotton variety FM 1621GL |
US11284595B2 (en) | 2019-02-12 | 2022-03-29 | BASF Agricultural Solutions Seed US LLC | Cotton variety FM 2398GLTP |
Families Citing this family (1282)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0242236B2 (en) * | 1986-03-11 | 1996-08-21 | Plant Genetic Systems N.V. | Plant cells resistant to glutamine synthetase inhibitors, made by genetic engineering |
CA1293701C (en) * | 1986-07-28 | 1991-12-31 | Peter J. Dart | Agricultural products and methods |
US5276268A (en) * | 1986-08-23 | 1994-01-04 | Hoechst Aktiengesellschaft | Phosphinothricin-resistance gene, and its use |
US5273894A (en) * | 1986-08-23 | 1993-12-28 | Hoechst Aktiengesellschaft | Phosphinothricin-resistance gene, and its use |
ES2038631T3 (en) * | 1986-08-23 | 1993-08-01 | Hoechst Aktiengesellschaft | PROCEDURE FOR OBTAINING A RESISTANCE GENE AGAINST PHOSPHINOTRICIN (PTC). |
US5637489A (en) * | 1986-08-23 | 1997-06-10 | Hoechst Aktiengesellschaft | Phosphinothricin-resistance gene, and its use |
EP0265556A1 (en) * | 1986-10-31 | 1988-05-04 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Stable binary agrobacterium vectors and their use |
CN87100603A (en) * | 1987-01-21 | 1988-08-10 | 昂科公司 | Vaccines against melanoma |
DE3715958A1 (en) * | 1987-05-13 | 1988-11-24 | Hoechst Ag | HERBICIDE-RESISTANT CULTURAL PLANTS, METHOD FOR THEIR SELECTION AND REGENERATION |
DE3732972A1 (en) * | 1987-07-02 | 1989-01-12 | Hoechst Ag | RESISTANCE GENES TO PHOSPHINOTHRICIN AND ITS USE |
FR2629098B1 (en) * | 1988-03-23 | 1990-08-10 | Rhone Poulenc Agrochimie | CHEMICAL GENE OF HERBICIDE RESISTANCE |
GB8825402D0 (en) * | 1988-10-31 | 1988-11-30 | Cambridge Advanced Tech | Sulfonamide resistance genes |
US6803499B1 (en) | 1989-08-09 | 2004-10-12 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
US5550318A (en) * | 1990-04-17 | 1996-08-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
US7705215B1 (en) | 1990-04-17 | 2010-04-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
ATE199098T1 (en) * | 1989-11-17 | 2001-02-15 | Monsanto Co | SOYBEAN PLANTS RESISTANT TO GLUTAMINE SYNTHETASE INHIBITORS |
JP3209744B2 (en) | 1990-01-22 | 2001-09-17 | デカルブ・ジェネティクス・コーポレーション | Transgenic corn with fruiting ability |
US6329574B1 (en) | 1990-01-22 | 2001-12-11 | Dekalb Genetics Corporation | High lysine fertile transgenic corn plants |
US5484956A (en) * | 1990-01-22 | 1996-01-16 | Dekalb Genetics Corporation | Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin |
US6946587B1 (en) * | 1990-01-22 | 2005-09-20 | Dekalb Genetics Corporation | Method for preparing fertile transgenic corn plants |
US6777589B1 (en) * | 1990-01-22 | 2004-08-17 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
US6025545A (en) * | 1990-01-22 | 2000-02-15 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
US5908810A (en) * | 1990-02-02 | 1999-06-01 | Hoechst Schering Agrevo Gmbh | Method of improving the growth of crop plants which are resistant to glutamine synthetase inhibitors |
US5739082A (en) * | 1990-02-02 | 1998-04-14 | Hoechst Schering Agrevo Gmbh | Method of improving the yield of herbicide-resistant crop plants |
DE4327056A1 (en) * | 1993-08-12 | 1995-02-16 | Hoechst Schering Agrevo Gmbh | Process for increasing the yield of herbicide-resistant crops |
US6395966B1 (en) * | 1990-08-09 | 2002-05-28 | Dekalb Genetics Corp. | Fertile transgenic maize plants containing a gene encoding the pat protein |
US5367110A (en) * | 1990-11-13 | 1994-11-22 | Yeda Research And Development Co. Ltd. | Transgenic plants overproducing threonine and lysine |
USRE36449E (en) * | 1991-03-05 | 1999-12-14 | Rhone-Poulenc Agro | Chimeric gene for the transformation of plants |
GB9225845D0 (en) * | 1992-12-10 | 1993-02-03 | Nickerson Biocem Ltd | Modified plants |
WO1994024264A1 (en) | 1993-04-09 | 1994-10-27 | Plant Genetic Systems N.V. | New bacillus thuringiensis strains and their insecticidal proteins |
US6281411B1 (en) | 1993-08-25 | 2001-08-28 | Dekalb Genetics Corporation | Transgenic monocots plants with increased glycine-betaine content |
US6118047A (en) | 1993-08-25 | 2000-09-12 | Dekalb Genetic Corporation | Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction |
US6326527B1 (en) * | 1993-08-25 | 2001-12-04 | Dekalb Genetics Corporation | Method for altering the nutritional content of plant seed |
US5780709A (en) * | 1993-08-25 | 1998-07-14 | Dekalb Genetics Corporation | Transgenic maize with increased mannitol content |
US6114608A (en) | 1997-03-14 | 2000-09-05 | Novartis Ag | Nucleic acid construct comprising bacillus thuringiensis cry1Ab gene |
GB9502468D0 (en) | 1995-02-09 | 1995-03-29 | Gene Shears Pty Ltd | DNA Sequence |
GB9506684D0 (en) * | 1995-03-31 | 1995-05-24 | Nickerson Biocem Ltd | Control of pod dehiscence |
EP0757102A1 (en) | 1995-08-04 | 1997-02-05 | Plant Genetic Systems N.V. | Genetic transformation using a PARP inhibitor |
AU1306097A (en) * | 1996-01-04 | 1997-08-01 | Ciba-Geigy Ag | Herbicidal composition and method of weed control |
US6472587B1 (en) | 1997-03-25 | 2002-10-29 | Basf Aktiengesellschaft | Production of a 5-(2-chloro-4-(trifluoromethyl)phenoxy)-2-nitrobenzoic acid or 7-chloro-3-methyllquinoline-8-carboxylic acid-tolerant plant by expressing an exogenous 5-(2-chloro-4-(trifluoromethyl)phenoxy)-2-nitrobenzoic acid or 7-chloro-3-methyllquinoline-8-carboxylic acid-binding antibody in the plant |
DE69837916T2 (en) | 1997-04-03 | 2008-02-28 | DeKalb Genetics Corp., DeKalb | USE OF GLYPHOSATE RESISTANT MAIN LINES |
US6040497A (en) * | 1997-04-03 | 2000-03-21 | Dekalb Genetics Corporation | Glyphosate resistant maize lines |
US5994623A (en) * | 1997-04-09 | 1999-11-30 | E. I. Du Pont De Nemours And Company | Corn 4-α-glucanotransferase |
WO1998050553A1 (en) | 1997-05-07 | 1998-11-12 | E.I. Du Pont De Nemours And Company | Starch biosynthetic enzymes |
BR9809967A (en) | 1997-06-06 | 2000-08-01 | Du Pont | Isolated nucleic acid fragment, chimeric gene, transformed host cell, polypeptide, method of altering the level of expression of an enzyme, method of obtaining a nucleic acid fragment, product and method for assessing the ability of at least one compound to inhibit the activity of an enzyme |
US7161064B2 (en) | 1997-08-12 | 2007-01-09 | North Carolina State University | Method for producing stably transformed duckweed using microprojectile bombardment |
US6521433B1 (en) | 1997-09-17 | 2003-02-18 | E. I. Du Pont De Nemours And Company | cDNA sequences from plants that encode activities associated with isoflavone biosynthesis |
WO1999018239A1 (en) * | 1997-10-02 | 1999-04-15 | Smithkline Beecham Corporation | Antimicrobial drug screening using a recombinant cell comprising a rna-dependent amidotransferase gene |
US5979854A (en) * | 1997-12-03 | 1999-11-09 | Lundgren; Curt | Strut apparatus for holding drywall panels and building materials in position |
WO1999041975A1 (en) | 1998-02-19 | 1999-08-26 | Cotton Incorporated | A method for the production of transgenic plants using apical shoot tips |
US6437223B1 (en) | 1998-03-13 | 2002-08-20 | Syngenta Participations Ag | Inbred maize line 2070BT |
NZ505988A (en) | 1998-03-20 | 2003-10-31 | Edgar B Cahoon | Delta-5 acyl-CoA desaturase and fatty acyl-CoA elongase and subsequent recombinant methods to produce seed oil comprising destaurated fatty acid |
CN1202246C (en) | 1998-04-08 | 2005-05-18 | 联邦科学和工业研究组织 | Methods for means for obtaining modified phenotypes |
US6693185B2 (en) | 1998-07-17 | 2004-02-17 | Bayer Bioscience N.V. | Methods and means to modulate programmed cell death in eukaryotic cells |
DE19836684A1 (en) | 1998-08-13 | 2000-02-17 | Hoechst Schering Agrevo Gmbh | Use of a synergistic herbicidal combination including a glufosinate- or glyphosate-type, imidazolinone or protoporphyrinogen oxidase to control weeds in rice |
DE19836660A1 (en) | 1998-08-13 | 2000-02-17 | Hoechst Schering Agrevo Gmbh | Use of a synergistic herbicide combination including a glufosinate- or glyphosate-type, imidazolinone or protoporphyrinogen oxidase inhibitory azole herbicide to control weeds in soya |
PT1104243E (en) | 1998-08-13 | 2013-05-07 | Bayer Cropscience Ag | Herbicides for tolerant or resistant corn cultures |
DE19836700A1 (en) | 1998-08-13 | 2000-02-17 | Hoechst Schering Agrevo Gmbh | Use of a synergistic herbicide combination including a glufosinate- or glyphosate-type, imidazolinone or protoporphyrinogen oxidase inhibitory azole herbicide to control weeds in cereals |
DE19836659A1 (en) | 1998-08-13 | 2000-02-17 | Hoechst Schering Agrevo Gmbh | Use of synergistic herbicide combination including glufosinate- or glyphosate-type, imidazolinone, protoporphyrinogen oxidase inhibitory azole or hydroxybenzonitrile herbicide, to control weeds in cotton |
DE19836673A1 (en) † | 1998-08-13 | 2000-02-17 | Hoechst Schering Agrevo Gmbh | Use of a synergistic herbicidal combination including a glufosinate- or glyphosate-type or imidazolinone herbicide to control weeds in sugar beet |
AU1336200A (en) * | 1998-11-03 | 2000-05-22 | Aventis Cropscience N.V. | Glufosinate tolerant rice |
US6333449B1 (en) | 1998-11-03 | 2001-12-25 | Plant Genetic Systems, N.V. | Glufosinate tolerant rice |
EP1177299B1 (en) | 1999-05-07 | 2008-10-29 | E.I. Du Pont De Nemours And Company | Auxin transport proteins |
US6483013B1 (en) | 1999-05-19 | 2002-11-19 | Bayer Bioscience N.V. | Method for agrobacterium mediated transformation of cotton |
US6451564B1 (en) | 1999-07-02 | 2002-09-17 | Massachusetts Institute Of Technology | Methods for producing L-isoleucine |
WO2001007061A1 (en) * | 1999-07-27 | 2001-02-01 | Smithkline Beecham Corporation | Whole cell assay |
US20060242729A1 (en) | 1999-07-30 | 2006-10-26 | Cahoon Edgar B | Polynucleotides encoding proteins involved in plant metabolism |
GB9918061D0 (en) | 1999-07-30 | 1999-10-06 | Univ Bath | Modified plants |
DE60043701D1 (en) | 1999-08-16 | 2010-03-04 | Du Pont | PROCESS FOR PREPARING CALENDULA ACID, A FATTY ACID WITH DELTA-8, 10, 12 CONJUGATED DOUBLE BINDINGS AND DIMORPHOLIC ACID, A FATTY ACID WITH A 9-HYDROXYL GROUP AND DELTA-10, 12 CONJUGATED DOUBLE BINDINGS |
GB9923306D0 (en) | 1999-10-01 | 1999-12-08 | Isis Innovation | Diagnostic and therapeutic epitope, and transgenic plant |
CA2389990C (en) | 1999-11-10 | 2007-05-29 | The University Of Washington | Compositions and methods for modulation of plant cell division |
US6943010B1 (en) | 1999-11-19 | 2005-09-13 | Hortense W. Dodo | Down-regulation and silencing of allergen genes in transgenic peanut seeds |
AU784649B2 (en) | 1999-12-28 | 2006-05-18 | Bayer Cropscience Nv | Insecticidal proteins from Bacillus thuringiensis |
FR2803484B1 (en) | 2000-01-06 | 2004-06-25 | Biogemma Fr | PROCESS FOR OBTAINING PLANTS WITH ENRICHED CYSTEIN AND GLUTATHIONE CONTENT |
US6831040B1 (en) | 2000-01-27 | 2004-12-14 | The Regents Of The University Of California | Use of prolines for improving growth and other properties of plants and algae |
EP1311659B1 (en) | 2000-08-22 | 2006-03-08 | E. I. du Pont de Nemours and Company | Nucleotide sequences of a new class of diverged delta-9 stearoyl-acp desaturase genes |
FR2815969B1 (en) | 2000-10-30 | 2004-12-10 | Aventis Cropscience Sa | TOLERANT PLANTS WITH HERBICIDES BY METABOLIC BYPASS |
GB0031558D0 (en) | 2000-12-22 | 2001-02-07 | Biogemma Uk Ltd | Elongase promoters |
EP2206703A1 (en) | 2008-12-30 | 2010-07-14 | Bayer CropScience AG | Pyrimidine derivatives and use thereof for combating undesired plant growth |
EP1988099B1 (en) | 2001-01-09 | 2012-11-14 | Bayer CropScience NV | Bacillus thuringiensis insecticidal proteins |
EP1362113B1 (en) | 2001-02-22 | 2011-01-12 | Biogemma | Constitutive promoter from arabidopsis |
GB0124574D0 (en) | 2001-10-12 | 2001-12-05 | Biogemma Uk Ltd | Oil biosynthesis |
EP2278017B1 (en) | 2002-02-26 | 2015-03-25 | Syngenta Limited | A method of selectively producing male or female sterile plants |
EP2360179A1 (en) | 2002-03-22 | 2011-08-24 | Bayer BioScience N.V. | Novel bacillus thuringiensis insecticidal proteins |
GB0212885D0 (en) | 2002-06-05 | 2002-07-17 | Isis Innovation | Therapeutic epitopes and uses thereof |
EP1371419A1 (en) * | 2002-06-12 | 2003-12-17 | F. Hoffmann-La Roche AG | Method and device for detecting the presence of an analyte in a test sample |
EP1517605A2 (en) | 2002-06-28 | 2005-03-30 | University of Guelph | Harvest-inducible genes from alfalfa (medicago sativa) and methods of use thereof |
US7329798B2 (en) * | 2002-06-28 | 2008-02-12 | University Of Guelph | Harvest-inducible regulatory elements and methods of using same |
EP1862473B1 (en) | 2002-07-31 | 2009-05-06 | Bayer BioScience N.V. | Corn root preferential promoters and uses thereof |
US7205454B2 (en) * | 2002-07-31 | 2007-04-17 | Bayer Bioscience N.V. | Corn root preferential promoters and uses thereof |
US7994400B2 (en) | 2002-08-16 | 2011-08-09 | Royster-Clark Resources, Llc | Plant seed mixtures |
US7476777B2 (en) | 2002-09-17 | 2009-01-13 | Ceres, Inc. | Biological containment system |
WO2004027038A2 (en) * | 2002-09-17 | 2004-04-01 | Ceres, Inc. | Biological containment system |
EP1592674B1 (en) | 2003-02-05 | 2014-03-05 | Bayer CropScience AG | Amino 1, 3, 5-triazines n-substituted with chiral bicyclic radicals, process for their preparation, compositions thereof and their use as herbicides and plant growth regulators |
US7365240B2 (en) | 2003-02-05 | 2008-04-29 | Divergence, Inc. | Nucleic acids encoding anthelmintic agents and plants made therefrom |
AU2004227360B2 (en) | 2003-04-04 | 2009-05-28 | Pioneer Hi-Bred International, Inc. | Modulation of cytokinin activity in plants |
CN1863914B (en) | 2003-04-29 | 2011-03-09 | 先锋高级育种国际公司 | Novel glyphosate-n-acetyltransferase (GAT) genes |
CN1871346B (en) | 2003-06-23 | 2013-05-29 | 先锋高级育种国际公司 | Engineering single-gene-controlled staygreen potential into plants |
SI2025756T1 (en) | 2003-11-18 | 2011-10-28 | Bayer Bioscience Nv | Improved targeted DNA insertion in plants |
EP1699291B1 (en) | 2003-12-24 | 2009-09-02 | Bayer CropScience AG | Plant growth regulation |
US7368629B2 (en) | 2004-02-04 | 2008-05-06 | Divergence, Inc. | Nucleic acids encoding anthelmintic agents and plants made therefrom |
DE102004016496A1 (en) | 2004-04-03 | 2005-10-20 | Bayer Cropscience Gmbh | Herbicidal 3-amino-2-thiomethylbenzoylpyrazole |
US10105437B2 (en) | 2004-04-28 | 2018-10-23 | Btg International Limited | Epitopes related to coeliac disease |
CA2564521C (en) | 2004-04-28 | 2017-04-11 | Btg International Limited | Epitopes related to coeliac disease |
EP2308977B2 (en) | 2004-04-30 | 2017-04-26 | Dow AgroSciences LLC | Novel herbicide resistance gene |
US8785731B2 (en) | 2004-09-30 | 2014-07-22 | Dow Agrosciences, Llc. | Canola plants with high oleic and low linolenic |
US7429692B2 (en) | 2004-10-14 | 2008-09-30 | Ceres, Inc. | Sucrose synthase 3 promoter from rice and uses thereof |
US20070199095A1 (en) | 2005-10-13 | 2007-08-23 | Edwards Allen | Methods for producing hybrid seed |
PL2039252T3 (en) | 2005-02-22 | 2011-12-30 | Basf Se | Composition and method for improving plant health |
US7968764B2 (en) | 2005-05-02 | 2011-06-28 | Purdue Research Foundation | Methods for increasing the yield of fermentable sugars from plant stover |
WO2007007147A2 (en) | 2005-07-08 | 2007-01-18 | Universidad Nacional Autonoma De Mexico Instituto De Biotecnologia | Novel bacterial proteins with pesticidal activity |
US8993846B2 (en) | 2005-09-06 | 2015-03-31 | Monsanto Technology Llc | Vectors and methods for improved plant transformation efficiency |
PL1941045T3 (en) | 2005-09-06 | 2011-09-30 | Stichting Wageningen Res | Use of a nucleic acid sequence for the generation of a transgenic plant having enhanced drought tolerance |
CN103361316B (en) | 2005-10-28 | 2017-05-17 | 美国陶氏益农公司 | Novel herbicide resistance genes |
WO2007055996A2 (en) | 2005-11-03 | 2007-05-18 | Liat Mintz | Compositions, reagents and kits for and methods of diagnosing, monitoring and treating hormonal imbalance |
DE102005057250A1 (en) | 2005-11-29 | 2007-06-06 | Bayer Cropscience Gmbh | Active ingredients to increase stress control in plants against abiotic stress and methods for their discovery |
NZ571115A (en) | 2006-03-02 | 2011-11-25 | Athenix Corp | Method and compositions for improved enzyme activity in transgenic plant |
WO2007107302A2 (en) | 2006-03-21 | 2007-09-27 | Bayer Bioscience N.V. | Novel genes encoding insecticidal proteins |
BRPI0709783B1 (en) | 2006-05-09 | 2017-05-30 | Univ Missouri | plant artificial minichromosomes produced by telomere truncation and method of production thereof |
EP2027275A2 (en) | 2006-05-16 | 2009-02-25 | Monsanto Technology, LLC | Use of non-agrobacterium bacterial species for plant transformation |
US7855326B2 (en) | 2006-06-06 | 2010-12-21 | Monsanto Technology Llc | Methods for weed control using plants having dicamba-degrading enzymatic activity |
BRPI0712484B1 (en) | 2006-06-06 | 2017-06-06 | Monsanto Technology Llc | method for selecting transformed cells |
BRPI0711953A2 (en) | 2006-06-08 | 2012-01-17 | Athenix Corp | isolated polynucleotide and polypeptide conferring resistance to glutamine synthetase and methods for producing transgenic plants and plant cells having improved nitrogen production and utilization |
US7951995B2 (en) | 2006-06-28 | 2011-05-31 | Pioneer Hi-Bred International, Inc. | Soybean event 3560.4.3.5 and compositions and methods for the identification and detection thereof |
US7928295B2 (en) * | 2006-08-24 | 2011-04-19 | Bayer Bioscience N.V. | Herbicide tolerant rice plants and methods for identifying same |
US7939721B2 (en) | 2006-10-25 | 2011-05-10 | Monsanto Technology Llc | Cropping systems for managing weeds |
CA2667117C (en) | 2006-11-10 | 2016-04-26 | Basf Se | Crystalline modification of fipronil |
AU2007316639B2 (en) | 2006-11-10 | 2012-12-20 | Basf Se | Crystalline modification of fipronil |
UA110598C2 (en) | 2006-11-10 | 2016-01-25 | Басф Се | Method of receiving crystalline modification of fipronil |
US8188136B2 (en) | 2006-11-10 | 2012-05-29 | Basf Se | Crystalline modification of fipronil |
ES2553331T3 (en) * | 2006-12-07 | 2015-12-07 | Dow Agrosciences Llc | New selectable marker genes |
EP2258177A3 (en) | 2006-12-15 | 2011-11-09 | Rohm and Haas Company | Mixtures comprising 1-methylcyclopropene |
DE102006059941A1 (en) | 2006-12-19 | 2008-06-26 | Bayer Cropscience Ag | Substituted 2,4-diamino-1,3,5-triazines, process for their preparation and their use as herbicides and plant growth regulators |
CL2007003744A1 (en) * | 2006-12-22 | 2008-07-11 | Bayer Cropscience Ag | COMPOSITION THAT INCLUDES A 2-PYRIDILMETILBENZAMIDE DERIVATIVE AND AN INSECTICIDE COMPOUND; AND METHOD TO CONTROL FITOPATOGENOS CULTURES AND INSECTS FACING OR PREVENTIVELY. |
CL2007003743A1 (en) * | 2006-12-22 | 2008-07-11 | Bayer Cropscience Ag | COMPOSITION THAT INCLUDES FENAMIDONA AND AN INSECTICIDE COMPOUND; AND METHOD TO CONTROL FITOPATOGENOS CULTURES AND INSECTS FACING OR PREVENTIVELY. |
EP2066177B1 (en) | 2007-01-19 | 2014-12-24 | Basf Se | Fungicidal mixtures of 1-methylpyrazole-4-ylcarboxylic acid anilides and azolopyrimidinylamines |
ATE549325T1 (en) | 2007-01-26 | 2012-03-15 | Basf Se | 3-AMINO-1,2-BENZISOTHIAZOLE COMPOUNDS TO FIGHT ANIMAL PLAGUE II |
EP1952691A3 (en) | 2007-01-31 | 2008-09-17 | Basf Se | Method for improving plant health by application of a triazolopyrimidine derivative |
EP1952690A3 (en) | 2007-01-31 | 2009-04-22 | Basf Se | Pesticidal mixtures based on triazolopyrimidines and insecticides |
WO2008095924A2 (en) | 2007-02-06 | 2008-08-14 | Basf Se | Insecticides as safener for fungicides with phytotoxic action |
US8445746B2 (en) | 2007-02-23 | 2013-05-21 | University Of Georgia Research Foundation, Inc. | Compositions and methods for identifying genetic sequences with toxin resistance in plants |
US7838729B2 (en) | 2007-02-26 | 2010-11-23 | Monsanto Technology Llc | Chloroplast transit peptides for efficient targeting of DMO and uses thereof |
EP3290520B1 (en) | 2007-03-09 | 2021-07-14 | Monsanto Technology LLC | Preparation and use of plant embryo explants for transformation |
EP1969934A1 (en) * | 2007-03-12 | 2008-09-17 | Bayer CropScience AG | 4-cycloalkyl or 4-aryl substituted phenoxy phenylamidines and their use as fungicides |
US20100167926A1 (en) * | 2007-03-12 | 2010-07-01 | Bayer Cropscience Ag | 3-substituted phenoxyphenylamidines and use thereof as fungicides |
BRPI0808798A2 (en) * | 2007-03-12 | 2014-10-07 | Bayer Cropscience Ag | 3,5-DISSUBSTITUTED PHENOXYPHENYLAMIDINS AND THEIR USE AS FUNGICIDES |
DE102007012168A1 (en) | 2007-03-12 | 2008-09-18 | Bayer Cropscience Ag | New thiazole derivatives useful as herbicides and plant growth regulators |
EP1969930A1 (en) * | 2007-03-12 | 2008-09-17 | Bayer CropScience AG | Phenoxy phenylamidines and their use as fungicides |
EP1969929A1 (en) * | 2007-03-12 | 2008-09-17 | Bayer CropScience AG | Substituted phenylamidines and their use as fungicides |
DE102007029603A1 (en) | 2007-06-27 | 2009-01-08 | Bayer Cropscience Ag | Phenylamidine for herbicide agent, controlling unwanted plants, and for use as herbicides, comprises general formula |
EP1969931A1 (en) * | 2007-03-12 | 2008-09-17 | Bayer CropScience Aktiengesellschaft | Fluoroalkyl phenylamidines and their use as fungicides |
BRPI0808786A2 (en) * | 2007-03-12 | 2014-09-16 | Bayer Cropscience Ag | DI-HALOGENOPHENOXYPHYMYLAMIDINES AND ITS USE AS FUNGICIDES |
CA2683735A1 (en) | 2007-04-12 | 2008-10-23 | Dow Agrosciences Llc | Novel canola cultivars having high yield and stabilized fatty acid profiles |
US7723577B2 (en) | 2007-04-12 | 2010-05-25 | Dow Agrosciences Llc | Canola cultivar DN040847 |
US7723580B2 (en) | 2007-04-12 | 2010-05-25 | Dow Agrosciences Llc | Canola cultivar DN040844 |
US7723581B2 (en) | 2007-04-12 | 2010-05-25 | Dow Agrosciences Llc | Canola cultivar DN040845 |
US7728195B2 (en) | 2007-04-12 | 2010-06-01 | Dow Agrosciences Llc | Canola cultivar DN040856 |
US7723582B2 (en) | 2007-04-12 | 2010-05-25 | Dow Agrosciences Llc | Canola cultivar DN041100 |
US7723578B2 (en) | 2007-04-12 | 2010-05-25 | Dow Agrosciences Llc | Canola cultivar DN040839 |
US7718852B2 (en) | 2007-04-12 | 2010-05-18 | Dow Agrosciences Llc | Canola cultivar DN040241 |
US7723579B2 (en) | 2007-04-12 | 2010-05-25 | Dow Agrosciences Llc | Canola cultivar DN040244 |
CN104206402B (en) | 2007-04-12 | 2018-04-24 | 巴斯夫欧洲公司 | Pesticide combination comprising cyanosulfoximine compounds |
EP1980150A1 (en) | 2007-04-13 | 2008-10-15 | Basf Se | Fungicidal mixtures based on triazolopyrimidine compounds |
JP2010524869A (en) | 2007-04-19 | 2010-07-22 | バイエル・クロツプサイエンス・アクチエンゲゼルシヤフト | Thiadiazolyloxyphenylamidines and their use as fungicides |
US8288315B2 (en) | 2007-04-25 | 2012-10-16 | Basf Se | Fungicide mixtures |
CN101688216B (en) | 2007-06-01 | 2014-03-26 | 拜尔作物科学公司 | Novel genes encoding insecticidal proteins |
DE102007026875A1 (en) | 2007-06-11 | 2008-12-24 | Bayer Cropscience Ag | 3-Cyclopropyl-4- (3-thiobenzoyl) pyrazoles and their use as herbicides |
WO2009029739A2 (en) | 2007-08-29 | 2009-03-05 | E. I. Du Pont De Nemours And Company | Methods involving genes encoding nucleoside diphosphatase kinase (ndk) polypeptides and homologs thereof for modifying the plant's root architecture |
WO2009035852A2 (en) | 2007-09-11 | 2009-03-19 | Monsanto Technology Llc | Increased alpha-prime beta-conglycinin soybeans |
JP2010539213A (en) | 2007-09-20 | 2010-12-16 | ビーエーエスエフ ソシエタス・ヨーロピア | Combinations containing bactericidal strains and active ingredients |
DE102007045955A1 (en) | 2007-09-26 | 2009-04-09 | Bayer Cropscience Ag | Active agent combination, useful e.g. for combating animal pests and treating seeds of transgenic plants, comprises substituted amino-furan-2-one compound and at least one compound e.g. diazinon, isoxathion, carbofuran or aldicarb |
DE102007045922A1 (en) * | 2007-09-26 | 2009-04-02 | Bayer Cropscience Ag | Drug combinations with insecticidal and acaricidal properties |
JP2010540495A (en) | 2007-09-26 | 2010-12-24 | ビーエーエスエフ ソシエタス・ヨーロピア | Three-component bactericidal composition comprising boscalid and chlorothalonil |
DE102007045953B4 (en) | 2007-09-26 | 2018-07-05 | Bayer Intellectual Property Gmbh | Drug combinations with insecticidal and acaricidal properties |
DE102007045956A1 (en) * | 2007-09-26 | 2009-04-09 | Bayer Cropscience Ag | Combination of active ingredients with insecticidal and acaricidal properties |
DE102007045919B4 (en) | 2007-09-26 | 2018-07-05 | Bayer Intellectual Property Gmbh | Drug combinations with insecticidal and acaricidal properties |
DE102007045957A1 (en) | 2007-09-26 | 2009-04-09 | Bayer Cropscience Ag | Active agent combination, useful e.g. for combating animal pests e.g. insects and treating seeds of transgenic plants, comprises substituted amino-furan-2-one compound and at least one compound e.g. benzoyl urea, buprofezin and cyromazine |
DE102007045920B4 (en) | 2007-09-26 | 2018-07-05 | Bayer Intellectual Property Gmbh | Synergistic drug combinations |
EP2090168A1 (en) | 2008-02-12 | 2009-08-19 | Bayer CropScience AG | Method for improving plant growth |
MX2010002746A (en) * | 2007-10-02 | 2010-06-01 | Bayer Cropscience Ag | Methods of improving plant growth. |
BRPI0817911B8 (en) | 2007-10-05 | 2022-06-28 | Dow Agrosciences Llc | PROCESSES FOR TRANSFER OF MOLECULAR SUBSTANCES INTO PLANT CELLS AND PROCESS FOR EXPRESSION OF A GENE |
EP2052604A1 (en) | 2007-10-24 | 2009-04-29 | Bayer CropScience AG | Salts of 2-lodo-N-[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)carbamoyl] benzol sulphonamide, method for its manufacture and its application as herbicide and plant growth regulator |
EP2052603A1 (en) | 2007-10-24 | 2009-04-29 | Bayer CropScience AG | Application of 2-lodo-N-[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)carbamoyl] benzol sulphonamide and/or its salts for inhibiting unwanted plant growth in selected agricultural crop cultures or non-cultivated land |
US8097712B2 (en) | 2007-11-07 | 2012-01-17 | Beelogics Inc. | Compositions for conferring tolerance to viral disease in social insects, and the use thereof |
EP2617831A3 (en) | 2007-11-20 | 2013-08-07 | E. I. du Pont de Nemours and Company | Plants with altered root architecture, related constructs and methods involving genes encoding leucine rich repeat kinase (llrk) polypeptides and homologs thereof |
EP2065374A1 (en) | 2007-11-30 | 2009-06-03 | Bayer CropScience AG | 2-(benzyl- and 1H-pyrazol-4-ylmethyl)sulfinyl-thiazol-derivatives as herbicides and plant growth regulators |
EP2065373A1 (en) | 2007-11-30 | 2009-06-03 | Bayer CropScience AG | Chiral 3-(benzylsulfinyl)-5,5-dimethyl-4,5-dihydroisoxazole and 5,5-dimethyl-3-[(1H-pyrazol-4-ylmethyl) sulfinyl]-4,5-dihydroisoxazole derivatives, methods for their preparation and their use as herbicides and plant growth regulators |
CA2704271C (en) | 2007-12-03 | 2016-07-19 | Syngenta Participations Ag | Engineering enzymatically susceptible phytases |
WO2009085982A1 (en) | 2007-12-19 | 2009-07-09 | Monsanto Technology Llc | Method to enhance yield and purity of hybrid crops |
DE102008006005A1 (en) | 2008-01-25 | 2009-07-30 | Bayer Cropscience Ag | New N-azinyl-N'-pyridylsulfonyl-urea compounds useful e.g. as herbicide, plant growth regulator and plant protection regulator and to combat undesirable plant growth e.g. Agrostis in special plant cultures e.g. wheat, barley and rye |
EP2072512A1 (en) | 2007-12-20 | 2009-06-24 | Bayer CropScience AG | Herbicide compounds based on N-Azinyl-N'-pyridylsulfonyl-ureas |
EP2072506A1 (en) | 2007-12-21 | 2009-06-24 | Bayer CropScience AG | Thiazolyloxyphenylamidine or thiadiazolyloxyphenylamidine und its use as fungicide |
EP2554674B1 (en) | 2007-12-21 | 2014-11-05 | Keygene N.V. | Trichome specific promoters |
PL2259675T3 (en) * | 2008-02-22 | 2016-03-31 | Basf Se | Fungicidal compositions comprising 3'-bromine-2,3,4,6'-tetramethoxy-2'-6-dimethylbenzophenone |
EP2103216A1 (en) | 2008-03-19 | 2009-09-23 | Bayer CropScience AG | Selected salts from 3-(5,6-dihydro-1,4,2-dioxazin-3-yl)-N-[(4,6-dimethoxypyrimidin-2-yl)carbamoyl] pyridine-2-sulfonamide, methods for their production and their usage as herbicides and plant growth regulators |
EP2105437A1 (en) | 2008-03-26 | 2009-09-30 | Bayer CropScience Aktiengesellschaft | 4-(3-Aminobenzoyl)-5-cyclopropylisoxazols as herbicides |
EP2110019A1 (en) | 2008-04-19 | 2009-10-21 | Bayer CropScience AG | Herbicidal compounds based on N-Azinyl-N'-phenylsulfonylureas |
EP2112149A1 (en) | 2008-04-22 | 2009-10-28 | Bayer CropScience Aktiengesellschaft | 2-[(1H-Pyrazol-4-ylmethyl)-sulfonyl]-oxazole derivatives, 2-[(1H-pyrazol-4-ylmethyl)-sulfanyl]-oxazole derivatives and chiral 2-[(1H-pyrazol-4-ylmethyl)-sulfinyl]-oxazole derivatives, method for production of same and their use as herbicides and plant growth regulators |
EP2112143A1 (en) | 2008-04-22 | 2009-10-28 | Bayer CropScience AG | 2-(benzylsulfonyl)-oxazol-derivatives, chiral 2-(benzylsulfinyl]-oxazol derivatives, 2-(benzylsulfanyl-oxazol) derivatives, process for their preparation, as well as their use as herbicide and plant growth regulators |
EP2113172A1 (en) * | 2008-04-28 | 2009-11-04 | Bayer CropScience AG | Method for improved utilisation of the production potential of transgene plants |
US7964774B2 (en) | 2008-05-14 | 2011-06-21 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV384196 |
US8829282B2 (en) | 2008-05-14 | 2014-09-09 | Monsanto Technology, Llc | Plants and seeds of spring canola variety SCV425044 |
US7935870B2 (en) | 2008-05-14 | 2011-05-03 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV354718 |
US7947877B2 (en) | 2008-05-14 | 2011-05-24 | Monosanto Technology LLC | Plants and seeds of spring canola variety SCV328921 |
EP2127521A1 (en) | 2008-05-29 | 2009-12-02 | Bayer CropScience Aktiengesellschaft | 4-(3-Alkylsulfinylbenzoyl)pyrazoles as herbicides |
CN102118966A (en) * | 2008-06-11 | 2011-07-06 | 陶氏益农公司 | Constructs for expressing herbicide tolerance genes, related plants, and related trait combinations |
EP2135865A1 (en) | 2008-06-17 | 2009-12-23 | Bayer CropScience AG | Substituted 1-(diazinyl)pyrazol-4-yl acetic acids, method for their production and their use as herbicides and plant growth regulators |
EA020599B1 (en) | 2008-07-04 | 2014-12-30 | Басф Се | Fungicidal mixtures comprising substituted 1-methylpyrazol-4-ylcarboxanilides and abamectin |
EP2145537A1 (en) | 2008-07-09 | 2010-01-20 | Bayer CropScience AG | Plant growth regulator |
EP2300617B1 (en) | 2008-07-16 | 2016-03-23 | Monsanto Technology LLC | Methods and vectors for producing transgenic plants |
US8697941B2 (en) | 2008-07-23 | 2014-04-15 | Pioneer Hi-Bred International, Inc. | Molecular markers linked to PPO inhibitor tolerance in soybeans |
US8748695B2 (en) | 2008-07-23 | 2014-06-10 | Pioneer Hi-Bred International, Inc. | Molecular markers linked to PPO inhibitor tolerance in soybeans |
EP2147919A1 (en) | 2008-07-24 | 2010-01-27 | Bayer CropScience Aktiengesellschaft | Heterocyclic substituted amides, method for their manufacture and their use as herbicides |
EP2315760B1 (en) | 2008-07-29 | 2013-03-06 | Basf Se | Piperazine compounds with herbicidal effect |
EP2168434A1 (en) | 2008-08-02 | 2010-03-31 | Bayer CropScience AG | Use of azols to increase resistance of plants of parts of plants to abiotic stress |
JP2011530276A (en) | 2008-08-08 | 2011-12-22 | バイエル・バイオサイエンス・エヌ・ヴェー | Methods for characterizing and identifying plant fibers |
KR20110044900A (en) | 2008-08-14 | 2011-05-02 | 바이엘 크롭사이언스 아게 | Insecticidal 4-phenyl-1H-pyrazole |
DE102008041695A1 (en) * | 2008-08-29 | 2010-03-04 | Bayer Cropscience Ag | Methods for improving plant growth |
EP2183969A3 (en) | 2008-10-29 | 2011-01-05 | Basf Se | Method for increasing the number of seedlings per number of sowed grains of seed |
AU2009296625B2 (en) | 2008-09-26 | 2015-06-11 | Basf Agrochemical Products B.V. | Herbicide-resistant AHAS-mutants and methods of use |
US20110183848A1 (en) | 2008-10-02 | 2011-07-28 | Basf Se | Piperazine Compounds With Herbicidal Effect |
US8367873B2 (en) | 2008-10-10 | 2013-02-05 | Bayer Cropscience Ag | Phenyl-substituted bicyclooctane-1,3-dione derivatives |
WO2010046423A2 (en) | 2008-10-22 | 2010-04-29 | Basf Se | Use of sulfonylurea herbicides on cultivated plants |
WO2010046422A2 (en) | 2008-10-22 | 2010-04-29 | Basf Se | Use of auxin type herbicides on cultivated plants |
EP2362723A1 (en) | 2008-11-04 | 2011-09-07 | Dow Agrosciences LLC | Omega-9 quality brassica juncea |
EP2210492A1 (en) | 2008-11-29 | 2010-07-28 | Bayer CropScience AG | Herbicide safener combination |
EP2191719A1 (en) | 2008-11-29 | 2010-06-02 | Bayer CropScience AG | Herbicide safener combination |
EP2191716A1 (en) | 2008-11-29 | 2010-06-02 | Bayer CropScience AG | Herbicide safener combination |
EP2191720A1 (en) | 2008-11-29 | 2010-06-02 | Bayer CropScience AG | Herbicide-safener combination |
US8846946B2 (en) | 2008-12-02 | 2014-09-30 | Bayer Cropscience Ag | Germinal alkoxy/alkylspirocyclic substituted tetramate derivatives |
US8389443B2 (en) | 2008-12-02 | 2013-03-05 | Bayer Cropscience Ag | Geminal alkoxy/alkylspirocyclic substituted tetramate derivatives |
EP2201838A1 (en) | 2008-12-05 | 2010-06-30 | Bayer CropScience AG | Active ingredient-beneficial organism combinations with insecticide and acaricide properties |
EP2194052A1 (en) | 2008-12-06 | 2010-06-09 | Bayer CropScience AG | Substituted 1.(1-thiazolyl)- and 1-(isothiazolyl)pyrazol-4-yl acetic acids, method for their production and their use as herbicides and plant growth regulators |
EP2376636A1 (en) | 2008-12-17 | 2011-10-19 | E. I. du Pont de Nemours and Company | Plants having altered agronomic characteristics under nitrogen limiting conditions and related constructs and methods involving genes encoding lnt9 polypeptides |
DE102008063561A1 (en) | 2008-12-18 | 2010-08-19 | Bayer Cropscience Ag | Hydrazides, process for their preparation and their use as herbicides and insecticides |
EP2198709A1 (en) | 2008-12-19 | 2010-06-23 | Bayer CropScience AG | Method for treating resistant animal pests |
EP2204366A1 (en) | 2008-12-19 | 2010-07-07 | Bayer CropScience AG | Herbicidal and insecticidal phenyl-substituted pyridazinones |
US20120004114A1 (en) | 2008-12-22 | 2012-01-05 | E. I. Du Pont De Nemours And Company And Pioneer Hi-Bred International | Nucleotide sequences encoding gsh1 polypeptides and methods of use |
WO2010075966A1 (en) | 2008-12-29 | 2010-07-08 | Bayer Cropscience Ag | Method for improved use of the production potential of genetically modified plants |
EP2204094A1 (en) | 2008-12-29 | 2010-07-07 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants Introduction |
EP2223602A1 (en) | 2009-02-23 | 2010-09-01 | Bayer CropScience AG | Method for improved utilisation of the production potential of genetically modified plants |
EP2210879A1 (en) | 2008-12-30 | 2010-07-28 | Bayer CropScience AG | Pyrimidine derivatives and use thereof for combating undesired plant growth |
EP2039772A2 (en) | 2009-01-06 | 2009-03-25 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants introduction |
EP2039771A2 (en) | 2009-01-06 | 2009-03-25 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants |
EP2039770A2 (en) | 2009-01-06 | 2009-03-25 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants |
US20110239315A1 (en) | 2009-01-12 | 2011-09-29 | Ulla Bonas | Modular dna-binding domains and methods of use |
EP2206723A1 (en) | 2009-01-12 | 2010-07-14 | Bonas, Ulla | Modular DNA-binding domains |
KR20110106448A (en) | 2009-01-19 | 2011-09-28 | 바이엘 크롭사이언스 아게 | Cyclic diones and their use as inseciticides, acaricides and/or fungicides |
ES2619279T3 (en) | 2009-01-22 | 2017-06-26 | Syngenta Participations Ag. | Hydroxyphenylpyruvate Dioxygenase mutant polypeptides and methods of use |
US9347046B2 (en) | 2009-01-22 | 2016-05-24 | Syngenta Participations Ag | Hydroxyphenylpyruvate dioxygenase polypeptides and methods of use |
EP2227951A1 (en) | 2009-01-23 | 2010-09-15 | Bayer CropScience AG | Application of enaminocarbonyl compounds for combating viruses transmitted by insects |
ES2524819T3 (en) | 2009-01-27 | 2014-12-12 | Basf Se | Seed Treatment Procedure |
ES2406131T3 (en) | 2009-01-28 | 2013-06-05 | Bayer Intellectual Property Gmbh | Fungicidal derivatives of N-cycloalkyl-N-bicyclomethylene-carboxamine |
AR075126A1 (en) | 2009-01-29 | 2011-03-09 | Bayer Cropscience Ag | METHOD FOR THE BEST USE OF THE TRANSGENIC PLANTS PRODUCTION POTENTIAL |
WO2010089244A1 (en) | 2009-02-03 | 2010-08-12 | Basf Se | Method for dressing seeds |
US8373025B2 (en) | 2009-02-09 | 2013-02-12 | Chromatin Germplasm, Llc | Herbicide resistant sorghum |
AR075573A1 (en) | 2009-02-11 | 2011-04-20 | Basf Se | DIMETHOMORPH AS A PESTICIDE PROTECTOR WITH PHYTO-TOXIC EFFECTS |
WO2010092014A2 (en) | 2009-02-11 | 2010-08-19 | Basf Se | Pesticidal mixtures |
CN102307478A (en) | 2009-02-11 | 2012-01-04 | 巴斯夫欧洲公司 | Pesticidal mixtures |
WO2010092031A2 (en) | 2009-02-11 | 2010-08-19 | Basf Se | Pesticidal mixtures |
BRPI1005355A2 (en) | 2009-02-11 | 2016-02-10 | Basf Se | mixtures, pesticide composition, method for pest control and / or for improving plant health, method for protecting plant propagation material against pests and plant propagation material |
CN102317259B (en) | 2009-02-17 | 2015-12-02 | 拜尔农科股份公司 | Fungicidal N-(phenylcycloalkyl) carboxylic acid amides, N-(benzylic cycloalkyl group) carboxylic acid amides and thiocarboxamide derivative |
EP2218717A1 (en) | 2009-02-17 | 2010-08-18 | Bayer CropScience AG | Fungicidal N-((HET)Arylethyl)thiocarboxamide derivatives |
TW201031331A (en) | 2009-02-19 | 2010-09-01 | Bayer Cropscience Ag | Pesticide composition comprising a tetrazolyloxime derivative and a fungicide or an insecticide active substance |
GB0903346D0 (en) | 2009-02-27 | 2009-04-08 | Cambridge Advanced Tech | Transgenic Plants |
WO2010101818A1 (en) | 2009-03-02 | 2010-09-10 | Pioneer Hi-Bred International, Inc. | Nac transcriptional activators involved in abiotic stress tolerance |
AU2010220293B2 (en) | 2009-03-04 | 2014-09-11 | Basf Se | 3-arylquinazolin-4-one compounds for combating invertebrate pests |
CA2752044A1 (en) | 2009-03-09 | 2010-09-16 | Stephen M. Allen | Drought tolerant plants and methods involving genes encoding type c3hc4 ring finger zinc-finger family polypeptides |
ES2632567T3 (en) | 2009-03-11 | 2017-09-14 | Bayer Intellectual Property Gmbh | Ketoenols substituted with haloalkylmenyloxyphenyl |
DE102009001469A1 (en) | 2009-03-11 | 2009-09-24 | Bayer Cropscience Ag | Improving utilization of productive potential of transgenic plant by controlling e.g. animal pest, and/or by improving plant health, comprises treating the transgenic plant with active agent composition comprising prothioconazole |
WO2010103065A1 (en) | 2009-03-11 | 2010-09-16 | Basf Se | Fungicidal compositions and their use |
WO2010106008A2 (en) | 2009-03-16 | 2010-09-23 | Basf Se | Fungicidal compositions comprising fluopyram and metrafenone |
DE102009001681A1 (en) | 2009-03-20 | 2010-09-23 | Bayer Cropscience Ag | Improving utilization of production potential of a transgenic plant by controlling animal pests, phytopathogenic fungi, microorganisms and/or improving plant health, comprises treating plant with a drug composition comprising iprovalicarb |
KR20120014241A (en) | 2009-03-20 | 2012-02-16 | 바스프 에스이 | Method for treatment of crop with an encapsulated pesticide |
EP2229813A1 (en) | 2009-03-21 | 2010-09-22 | Bayer CropScience AG | Pyrimidine-4-ylpropandinitrile derivatives, method for their manufacture and their use as herbicides and plant growth regulators |
DE102009001732A1 (en) | 2009-03-23 | 2010-09-30 | Bayer Cropscience Ag | Improving the production potential of transgenic plant, by combating e.g. animal pests and/or microorganism, and/or increasing plant health, comprises treating the plants with active agent composition comprising trifloxystrobin |
DE102009001730A1 (en) | 2009-03-23 | 2010-09-30 | Bayer Cropscience Ag | Improving utilization of production potential of a transgenic plant by controlling animal pests, phytopathogenic fungi and/or microorganisms and/or the plant health, comprises treating plant with a drug composition comprising spiroxamine |
DE102009001728A1 (en) | 2009-03-23 | 2010-09-30 | Bayer Cropscience Ag | Improving the production potential of transgenic plant, by combating e.g. animal pests and/or microorganism, and/or increasing plant health, comprises treating the plants with active agent composition comprising fluoxastrobin |
EA020314B9 (en) | 2009-03-25 | 2015-03-31 | Байер Кропсайенс Аг | Pesticidal combinations of biologically active ingredients |
BRPI0924839B1 (en) | 2009-03-25 | 2018-03-20 | Bayer Intellectual Property Gmbh | Active substance combinations with insecticidal and acaricidal properties, their uses and method for controlling animal pests |
EP2410849A1 (en) | 2009-03-25 | 2012-02-01 | Bayer CropScience AG | Active ingredient combinations having insecticidal and acaricidal properties |
EP2232995A1 (en) | 2009-03-25 | 2010-09-29 | Bayer CropScience AG | Method for improved utilisation of the production potential of transgenic plants |
NZ595345A (en) | 2009-03-25 | 2014-01-31 | Bayer Cropscience Ag | Active ingredient combinations with insecticidal and acaricidal properties |
JP5462354B2 (en) | 2009-03-25 | 2014-04-02 | バイエル・クロップサイエンス・アーゲー | Active ingredient combinations with insecticidal and acaricidal properties |
NZ594887A (en) | 2009-03-26 | 2013-11-29 | Basf Se | Use of synthetic and biological fungicides in combination for controlling harmful fungi |
CN102369199A (en) | 2009-04-01 | 2012-03-07 | 巴斯夫欧洲公司 | Isoxazoline compounds for combating invertebrate pests |
US9232785B2 (en) | 2009-04-02 | 2016-01-12 | Basf Se | Method for reducing sunburn damage in plants |
EP2239331A1 (en) | 2009-04-07 | 2010-10-13 | Bayer CropScience AG | Method for improved utilization of the production potential of transgenic plants |
US8071864B2 (en) | 2009-04-15 | 2011-12-06 | Monsanto Technology Llc | Plants and seeds of corn variety CV897903 |
US8071865B2 (en) | 2009-04-15 | 2011-12-06 | Monsanto Technology Llc | Plants and seeds of corn variety CV589782 |
US8362332B2 (en) | 2009-04-15 | 2013-01-29 | Monsanto Technology Llc | Plants and seeds of corn variety CV165560 |
EP2245935A1 (en) | 2009-05-02 | 2010-11-03 | Bayer CropScience AG | Herbicide compounds based on N-Azinyl-N-pyridylsulfonyl-uric substances |
JP5771189B2 (en) | 2009-05-06 | 2015-08-26 | バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングBayer Intellectual Property GmbH | Cyclopentanedione compounds and their use as insecticides, acaricides and / or antifungal agents |
AR076839A1 (en) | 2009-05-15 | 2011-07-13 | Bayer Cropscience Ag | FUNGICIDE DERIVATIVES OF PIRAZOL CARBOXAMIDAS |
EP2251331A1 (en) | 2009-05-15 | 2010-11-17 | Bayer CropScience AG | Fungicide pyrazole carboxamides derivatives |
JP5892927B2 (en) | 2009-05-19 | 2016-03-23 | バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングBayer Intellectual Property GmbH | Spiroheterocyclic tetronic acid derivatives with herbicidal activity |
EP2255626A1 (en) | 2009-05-27 | 2010-12-01 | Bayer CropScience AG | Use of succinate dehydrogenase inhibitors to increase resistance of plants or parts of plants to abiotic stress |
PL2437595T3 (en) * | 2009-06-02 | 2019-05-31 | Bayer Cropscience Ag | Use of fluopyram for controlling sclerotinia ssp |
MX2011013011A (en) | 2009-06-05 | 2012-02-28 | Univ Florida | Isolation and targeted suppression of lignin biosynthetic genes from sugarcane. |
BRPI1010843A2 (en) | 2009-06-08 | 2015-09-08 | Nunhems Bv | drought tolerant plants |
US20120047603A1 (en) | 2009-06-09 | 2012-02-23 | Allen Stephen M | Drought tolerant plants and related constructs and methods involving genes encoding fatty acid desaturase family polypeptides |
EP2440663A1 (en) | 2009-06-09 | 2012-04-18 | Pioneer Hi-Bred International Inc. | Early endosperm promoter and methods of use |
US20120077676A1 (en) | 2009-06-12 | 2012-03-29 | Basf Se | Antifungal 1,2,4-Triazolyl Derivatives Having a 5-Sulfur Substituent |
WO2019106641A2 (en) | 2017-12-03 | 2019-06-06 | Seedx Technologies Inc. | Systems and methods for sorting of seeds |
US8071848B2 (en) | 2009-06-17 | 2011-12-06 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV218328 |
EP2442653A2 (en) | 2009-06-18 | 2012-04-25 | Basf Se | Fungicidal mixtures |
EP2443099A1 (en) | 2009-06-18 | 2012-04-25 | Basf Se | Antifungal 1, 2, 4-triazolyl derivatives having a 5- sulfur substituent |
EP2443097A1 (en) | 2009-06-18 | 2012-04-25 | Basf Se | Antifungal 1, 2, 4-triazolyl derivatives |
EP2443098A1 (en) | 2009-06-18 | 2012-04-25 | Basf Se | Antifungal 1, 2, 4-triazolyl derivatives |
KR20120062679A (en) | 2009-06-18 | 2012-06-14 | 바스프 에스이 | Triazole compounds carrying a sulfur substituent |
WO2010146116A1 (en) | 2009-06-18 | 2010-12-23 | Basf Se | Triazole compounds carrying a sulfur substituent |
WO2010146115A1 (en) | 2009-06-18 | 2010-12-23 | Basf Se | Triazole compounds carrying a sulfur substituent |
SI2443102T1 (en) | 2009-06-19 | 2013-08-30 | Basf Se | Herbicidal benzoxazinones |
WO2010149758A1 (en) | 2009-06-25 | 2010-12-29 | Basf Se | Antifungal 1, 2, 4-triazolyl derivatives |
AR077228A1 (en) | 2009-06-25 | 2011-08-10 | Basf Se | USE OF AGROCHEMICAL MIXTURES TO INCREASE PLANT HEALTH |
AU2010273756A1 (en) | 2009-06-30 | 2011-11-24 | E. I. Du Pont De Nemours And Company | Plant seeds with altered storage compound levels, related constructs and methods involving genes encoding cytosolic pyrophosphatase |
WO2011000498A1 (en) | 2009-07-01 | 2011-01-06 | Bayer Bioscience N.V. | Methods and means for obtaining plants with enhanced glyphosate tolerance |
EP2451804B1 (en) | 2009-07-06 | 2014-04-30 | Basf Se | Pyridazine compounds for controlling invertebrate pests |
WO2011003775A2 (en) | 2009-07-09 | 2011-01-13 | Basf Se | Substituted cyanobutyrates having a herbicidal effect |
WO2011003776A2 (en) | 2009-07-09 | 2011-01-13 | Basf Se | Substituted cyanobutyrates having a herbicidal effect |
BR112012001001A2 (en) | 2009-07-14 | 2016-11-16 | Basf Se | azole compounds of formulas i and ii, compounds of formulas i and i, compounds of formula ix, agricultural composition, use of a pharmaceutical compound, method for treating cancer or virus infections to combat zoopathogenic or humanopathogenic fungi |
EP2453750A2 (en) | 2009-07-16 | 2012-05-23 | Bayer CropScience AG | Synergistic active substance combinations containing phenyl triazoles |
WO2011009804A2 (en) | 2009-07-24 | 2011-01-27 | Basf Se | Pyridine derivatives compounds for controlling invertebrate pests |
US20120129696A1 (en) | 2009-07-28 | 2012-05-24 | Basf Se | Method for increasing the level of free amino acids in storage tissues of perennial plants |
MX2012000421A (en) | 2009-07-28 | 2012-02-08 | Basf Se | Pesticidal suspo-emulsion compositions. |
WO2011012246A1 (en) | 2009-07-29 | 2011-02-03 | Bayer Cropscience Ag | 4-(3-alkylthiobenzoyl)pyrazoles and use thereof as herbicides |
WO2011012248A2 (en) | 2009-07-29 | 2011-02-03 | Bayer Cropscience Ag | 2-(3-aminobenzoyl)-3-cyclopropyl-3-oxopropane nitriles and use thereof as herbicides |
IN2012DN00773A (en) | 2009-07-29 | 2015-06-26 | Bayer Cropscience Ag | |
WO2011014660A1 (en) | 2009-07-30 | 2011-02-03 | Merial Limited | Insecticidal 4-amino-thieno[2,3-d]-pyrimidine compounds and methods of their use |
WO2011015524A2 (en) | 2009-08-03 | 2011-02-10 | Bayer Cropscience Ag | Fungicide heterocycles derivatives |
UY32838A (en) | 2009-08-14 | 2011-01-31 | Basf Se | "HERBICIDE ACTIVE COMPOSITION THAT BENZOXAZINONAS UNDERSTANDS |
CA2770854A1 (en) | 2009-08-20 | 2011-02-24 | Pioneer Hi-Bred International, Inc. | Functional expression of shuffled yeast nitrate transporter (ynt1) in maize to improve nitrate uptake under low nitrate environment |
CA2770872A1 (en) | 2009-08-20 | 2011-02-24 | Pioneer Hi-Bred International, Inc. | Functional expression of yeast nitrate transporter (ynt1) in maize to improve nitrate uptake |
EP2292094A1 (en) | 2009-09-02 | 2011-03-09 | Bayer CropScience AG | Active compound combinations |
RU2624025C2 (en) | 2009-09-17 | 2017-06-30 | МОНСАНТО ТЕКНОЛОДЖИ ЭлЭлСи | Coi mon 87708 transgenic object and methods for its application |
US8440891B2 (en) | 2009-09-22 | 2013-05-14 | Board of Trustees of the University of Akransas, N.A. | Rice cultivar CL 142-AR |
JP2013505909A (en) | 2009-09-24 | 2013-02-21 | ビーエーエスエフ ソシエタス・ヨーロピア | Aminoquinazoline compounds for combating invertebrate pests |
WO2011035878A1 (en) | 2009-09-25 | 2011-03-31 | Bayer Cropscience Ag | Herbicidally effective phenyl-substituted pyridazinones |
US20120178625A1 (en) | 2009-09-25 | 2012-07-12 | Basf Se | Method for reducing pistillate flower abortion in plants |
AU2010303120A1 (en) | 2009-09-29 | 2012-04-19 | Basf Se | Pesticidal mixtures |
WO2011039276A1 (en) | 2009-10-01 | 2011-04-07 | Bayer Cropscience Ag | Oxathiazinyl(het)arylsulfonylureas, processes and intermediates for preparation thereof and use thereof as crop protection agents and crop growth regulators |
CA2775093A1 (en) | 2009-10-02 | 2011-04-07 | Pioneer Hi-Bred International, Inc. | Down-regulation of acc synthase for improved plant performance |
WO2011042378A1 (en) | 2009-10-09 | 2011-04-14 | Basf Se | Substituted cyanobutyrates having herbicidal effect |
US8962584B2 (en) | 2009-10-14 | 2015-02-24 | Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. | Compositions for controlling Varroa mites in bees |
WO2011045271A1 (en) | 2009-10-15 | 2011-04-21 | Bayer Cropscience Ag | Herbicidally active, heterocyclyl-substituted pyridazinones |
US8440892B2 (en) | 2009-10-15 | 2013-05-14 | Board Of Trustees Of The University Of Arkansas, N.A. | Rice cultivar CL 181-AR |
IN2012DN02333A (en) | 2009-10-16 | 2015-08-21 | Dow Agrosciences Llc | |
US8604280B2 (en) | 2009-10-19 | 2013-12-10 | J.R. Simplot Company | Glyphosate tolerant perennial ryegrass named ‘replay’ |
US8604279B2 (en) | 2009-10-19 | 2013-12-10 | J.R. Simplot Company | Glyphosate tolerant perennial ryegrass named ‘JS501’ |
WO2011050271A1 (en) | 2009-10-23 | 2011-04-28 | Monsanto Technology Llc | Methods and compositions for expression of transgenes in plants |
CA2775146A1 (en) | 2009-10-26 | 2011-05-12 | Pioneer Hi-Bred International, Inc. | Somatic ovule specific promoter and methods of use |
DE102010042867A1 (en) | 2009-10-28 | 2011-06-01 | Basf Se | Use of heterocyclic compounds as herbicides and for controlling undesirable plants in culture of useful plants e.g. wheat, barley, rye, oats, millet and rice |
WO2011051212A1 (en) | 2009-10-28 | 2011-05-05 | Basf Se | Use of heteroaromatic compounds as herbicides |
DE102010042864A1 (en) | 2009-10-30 | 2011-06-01 | Basf Se | Substituted thioamides with herbicidal activity |
AR078829A1 (en) | 2009-10-30 | 2011-12-07 | Du Pont | PLANTS AND SEEDS WITH ALTERED LEVELS OF STORAGE COMPOUND, RELATED CONSTRUCTIONS AND METHODS RELATED TO GENES THAT CODIFY SIMILAR PROTEINS TO THE BACTERIAL ALDOLASES OF CLASS II OF THE ACID 2,4- DIHYDROXI-HEPT-2-ENO-1,7-DIO-1,7 |
WO2011051393A1 (en) | 2009-11-02 | 2011-05-05 | Basf Se | Herbicidal tetrahydrophthalimides |
US8329619B2 (en) | 2009-11-03 | 2012-12-11 | Basf Se | Substituted quinolinones having herbicidal action |
JP2013510113A (en) | 2009-11-06 | 2013-03-21 | ビーエーエスエフ ソシエタス・ヨーロピア | Crystalline complex of 4-hydroxybenzoic acid and selected pesticide |
WO2011057989A1 (en) | 2009-11-11 | 2011-05-19 | Basf Se | Heterocyclic compounds having herbicidal action |
WO2011057942A1 (en) | 2009-11-12 | 2011-05-19 | Basf Se | Insecticidal methods using pyridine compounds |
WO2011058036A1 (en) | 2009-11-13 | 2011-05-19 | Basf Se | Tricyclic compounds having herbicidal action |
WO2011057935A1 (en) | 2009-11-13 | 2011-05-19 | Basf Se | 3-(3,4-dihydro-2h-benzo [1,4]oxazin-6-yl)-1h-pyrimidin-2,4-dione compounds as herbicides |
US9023874B2 (en) | 2009-11-17 | 2015-05-05 | Merial, Inc. | Fluorinated oxa or thia heteroarylalkylsulfide derivatives for combating invertebrate pests |
EP2327700A1 (en) | 2009-11-21 | 2011-06-01 | Bayer CropScience AG | Dialkyl triazinamines and use thereof for combating undesired plant growth |
EP2504442B1 (en) | 2009-11-24 | 2014-07-16 | Katholieke Universiteit Leuven, K.U. Leuven R&D | Banana promoters |
AU2010325563B2 (en) | 2009-11-27 | 2017-02-02 | Basf Plant Science Company Gmbh | Chimeric endonucleases and uses thereof |
WO2011064188A1 (en) | 2009-11-27 | 2011-06-03 | Basf Se | Insecticidal methods using nitrogen-containing heteroaromatic compounds |
WO2011064736A1 (en) | 2009-11-27 | 2011-06-03 | Basf Plant Science Company Gmbh | Optimized endonucleases and uses thereof |
CN102762726A (en) | 2009-11-27 | 2012-10-31 | 巴斯夫植物科学有限公司 | Chimeric endonucleases and uses thereof |
WO2011067184A1 (en) | 2009-12-01 | 2011-06-09 | Basf Se | 3- (4, 5 -dihydroisoxazol- 5 -yl) benzoylpyrazole compounds and mixtures thereof with safeners |
ES2546100T3 (en) | 2009-12-04 | 2015-09-18 | Merial, Inc. | Bis-organosulfurized pesticide compounds |
WO2011069912A1 (en) | 2009-12-07 | 2011-06-16 | Basf Se | Triazole compounds, use thereof and agents containing said compounds |
WO2011069955A1 (en) | 2009-12-07 | 2011-06-16 | Basf Se | Sulfonimidamide compounds for combating animal pests |
WO2011069916A1 (en) | 2009-12-08 | 2011-06-16 | Basf Se | Triazole compounds, use thereof as a fungicide, and agents comprising same |
EA025427B1 (en) | 2009-12-08 | 2016-12-30 | Басф Се | Pesticidal mixtures |
CN102638989B (en) | 2009-12-08 | 2015-01-28 | 巴斯夫欧洲公司 | Pesticidal mixtures |
WO2011069894A1 (en) | 2009-12-08 | 2011-06-16 | Basf Se | Triazole compounds, use thereof, and agents containing same |
EP2343280A1 (en) | 2009-12-10 | 2011-07-13 | Bayer CropScience AG | Fungicide quinoline derivatives |
WO2011072246A2 (en) | 2009-12-10 | 2011-06-16 | Regents Of The University Of Minnesota | Tal effector-mediated dna modification |
WO2011073098A1 (en) | 2009-12-15 | 2011-06-23 | Bayer Cropscience Ag | 1-(heteroaryl)-pyrazol-4-yl-acetic acids, method for the production thereof, and the use thereof as herbicides and plant growth regulators |
WO2011082953A2 (en) | 2009-12-17 | 2011-07-14 | Bayer Cropscience Ag | Herbicidal agents comprising flufenacet |
WO2011082956A2 (en) | 2009-12-17 | 2011-07-14 | Bayer Cropscience Ag | Herbicidal agents containing flufenacet |
LT2512249T (en) | 2009-12-17 | 2016-09-26 | Bayer Intellectual Property Gmbh | Herbicides comprising flufenacet |
ES2601837T3 (en) | 2009-12-17 | 2017-02-16 | Bayer Intellectual Property Gmbh | Herbicidal agents containing flufenacet |
WO2011082959A2 (en) | 2009-12-17 | 2011-07-14 | Bayer Cropscience Ag | Herbicidal agents containing flufenacet |
WO2011082964A1 (en) | 2009-12-17 | 2011-07-14 | Bayer Cropscience Ag | Herbicidal agents containing flufenacet |
WO2011082954A2 (en) | 2009-12-17 | 2011-07-14 | Bayer Cropscience Ag | Herbicidal agents containing flufenacet |
WO2011082966A2 (en) | 2009-12-17 | 2011-07-14 | Bayer Cropscience Ag | Herbicidal agents containing flufenacet |
WO2011082955A2 (en) | 2009-12-17 | 2011-07-14 | Bayer Cropscience Ag | Herbicidal agents comprising flufenacet |
WO2011082968A2 (en) | 2009-12-17 | 2011-07-14 | Bayer Cropscience Ag | Herbicidal agents containing flufenacet |
WO2011082957A2 (en) | 2009-12-17 | 2011-07-14 | Bayer Cropscience Ag | Herbicidal agents containing flufenacet |
US20120291159A1 (en) | 2009-12-18 | 2012-11-15 | Basf Se | Azoline Compounds for Combating Invertebrate Pests |
WO2011073143A1 (en) | 2009-12-18 | 2011-06-23 | Basf Se | Substituted cyanobutyrates having herbicidal action |
BR112012015697A2 (en) | 2009-12-23 | 2015-08-25 | Bayer Intelectual Property Gmbh | Herbicide tolerant plants of hppd inhibitors. |
UY33140A (en) | 2009-12-23 | 2011-07-29 | Bayer Cropscience Ag | TOLERANT PLANTS TO INHIBITING HERBICIDES OF HPPD |
UY33141A (en) | 2009-12-23 | 2011-07-29 | Bayer Cropscience Ag | TOLERANT PLANTS TO INHIBITING HERBICIDES OF HPPD |
CN102762725A (en) | 2009-12-23 | 2012-10-31 | 拜尔知识产权有限公司 | Plants tolerant to hppd inhibitor herbicides |
ES2658990T3 (en) | 2009-12-23 | 2018-03-13 | Bayer Intellectual Property Gmbh | HPPD-inhibiting herbicide-tolerant plants |
TWI528898B (en) | 2009-12-28 | 2016-04-11 | 拜耳知識產權公司 | Fungicide hydroximoyl-heterocycles derivatives |
BR112012012340A2 (en) | 2009-12-28 | 2015-09-08 | Bayer Cropscience Ag | compost, fungicidal composition and method for the control of plant pathogenic fungus |
JP5782658B2 (en) | 2009-12-28 | 2015-09-24 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | Fungicide hydroxymoyl-tetrazole derivative |
CN102711456B (en) | 2010-01-18 | 2015-05-13 | 巴斯夫欧洲公司 | Compound comprising a pesticide and an alkoxylate of 2-propylheptyl amine |
WO2011089071A2 (en) * | 2010-01-22 | 2011-07-28 | Bayer Cropscience Ag | Acaricide and/or insecticide active substance combinations |
WO2011094199A1 (en) * | 2010-01-26 | 2011-08-04 | Pioneer Hi-Bred International, Inc. | Polynucleotide and polypeptide sequences associated with herbicide tolerance |
JP2013518084A (en) | 2010-02-01 | 2013-05-20 | ビーエーエスエフ ソシエタス・ヨーロピア | Substituted ketonic isoxazoline compounds and derivatives for controlling pests |
EP2531603A2 (en) | 2010-02-02 | 2012-12-12 | E.I. Du Pont De Nemours And Company | Plants with altered root architecture, related constructs and methods involving genes encoding lectin protein kinase (lpk) polypeptides and homologs thereof |
US8378177B2 (en) | 2010-02-03 | 2013-02-19 | Dow Agrosciences, Llc | Canola cultivar DN051493 |
ES2545113T3 (en) | 2010-02-10 | 2015-09-08 | Bayer Intellectual Property Gmbh | Tetramic acid derivatives substituted in a spiroheterocyclic manner |
WO2011098417A1 (en) | 2010-02-10 | 2011-08-18 | Basf Se | Substituted cyanobutyrates having herbicidal action |
CN102834378B (en) | 2010-02-10 | 2016-07-06 | 拜耳知识产权有限责任公司 | The ring-type keto-enol that xenyl replaces |
WO2011101303A2 (en) | 2010-02-16 | 2011-08-25 | Basf Se | Compound comprising a pesticide and an alkoxylate of isoheptadecylamine |
CN102781235B (en) | 2010-02-19 | 2015-04-22 | 拜耳知识产权有限责任公司 | 3-aminocarbonyl-substituted benzoylcyclohexanediones and their use as herbicides |
US8148611B2 (en) | 2010-02-26 | 2012-04-03 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV453784 |
US8143488B2 (en) | 2010-02-26 | 2012-03-27 | Monsanto Technoloy LLC | Plants and seeds of spring canola variety SCV470336 |
US8138394B2 (en) | 2010-02-26 | 2012-03-20 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV431158 |
WO2011109618A2 (en) | 2010-03-03 | 2011-09-09 | E. I. Du Pont De Nemours And Company | Plant seeds with altered storage compound levels, related constructs and methods involving genes encoding oxidoreductase motif polypeptides |
WO2011107445A1 (en) | 2010-03-04 | 2011-09-09 | Bayer Cropscience Ag | Hydrate and anhydrous crystal form of the sodium salt of 2-iodo-n-[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)carbamoyl]benzenesulfonamide, process for preparation thereof and use thereof as herbicides and plant growth regulators |
US20110218103A1 (en) | 2010-03-04 | 2011-09-08 | Bayer Cropscience Ag | Fluoroalkyl-substituted 2-amidobenzimidazoles |
US20130047297A1 (en) | 2010-03-08 | 2013-02-21 | Robert D. Sammons | Polynucleotide molecules for gene regulation in plants |
US8581048B2 (en) | 2010-03-09 | 2013-11-12 | Monsanto Technology, Llc | Plants and seeds of spring canola variety SCV119103 |
WO2011110583A2 (en) | 2010-03-10 | 2011-09-15 | Basf Se | Fungicidal mixtures comprising triazole derivatives |
US8153865B2 (en) | 2010-03-11 | 2012-04-10 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV152154 |
EP2366791A1 (en) | 2010-03-16 | 2011-09-21 | Biogemma | Method for modifying the flowering date of a plant |
WO2011113786A2 (en) | 2010-03-17 | 2011-09-22 | Basf Se | Compound comprising a pesticide and an alkoxylate of branched nonyl amine |
WO2011113861A2 (en) | 2010-03-18 | 2011-09-22 | Bayer Cropscience Ag | Aryl and hetaryl sulfonamides as active agents against abiotic plant stress |
BR112012023757B1 (en) | 2010-03-23 | 2020-10-20 | Basf Se | pyridazine compound, method to control invertebrate pests and method to protect plant propagation material |
CN102834391A (en) | 2010-03-23 | 2012-12-19 | 巴斯夫欧洲公司 | Pyridazine compounds for controlling invertebrate pests |
CN102858780A (en) | 2010-03-23 | 2013-01-02 | 巴斯夫欧洲公司 | Substituted pyridazines having herbicidal action |
CN102822178B (en) | 2010-03-23 | 2015-10-21 | 巴斯夫欧洲公司 | There is the pyrido thiazine of herbicide effect |
EP2550271A1 (en) | 2010-03-23 | 2013-01-30 | Basf Se | Substituted pyridines having herbicidal action |
AR081526A1 (en) | 2010-03-23 | 2012-10-03 | Basf Se | PIRIDAZINAS REPLACED THAT HAVE HERBICITY ACTION |
EP2550264B1 (en) | 2010-03-23 | 2016-06-08 | Basf Se | Pyridazine compounds for controlling invertebrate pests |
JP2013522339A (en) | 2010-03-23 | 2013-06-13 | ビーエーエスエフ ソシエタス・ヨーロピア | Substituted pyridines with herbicidal action |
JP2013522335A (en) | 2010-03-23 | 2013-06-13 | ビーエーエスエフ ソシエタス・ヨーロピア | Pyrazinothiazine with herbicidal activity |
WO2011117184A1 (en) | 2010-03-24 | 2011-09-29 | Bayer Cropscience Ag | Fludioxonil derivates |
CN102933073B (en) | 2010-03-31 | 2015-11-25 | 陶氏益农公司 | For biomolecule delivery being entered the plant peptide GAMMA-zeins in plant cell |
EP2371823A1 (en) | 2010-04-01 | 2011-10-05 | Bayer CropScience AG | Cyclopropyl-substituted phenylsulfonylamino(thio)carbonyltriazolinones, their production and use as herbicides and plant growth regulators |
EP2555619A2 (en) | 2010-04-06 | 2013-02-13 | Bayer Intellectual Property GmbH | Use of 4-phenylbutyric acid and/or the salts thereof for enhancing the stress tolerance of plants |
BR112012025848A2 (en) | 2010-04-09 | 2015-09-08 | Bayer Ip Gmbh | The use of (1-cyanocyclopropyl) phenylphosphinic acid derivatives, its esters and / or salts thereof to increase the tolerance of plants to abiotic stress. |
WO2011134913A1 (en) | 2010-04-28 | 2011-11-03 | Bayer Cropscience Ag | Fungicide hydroximoyl-heterocycles derivatives |
WO2011134911A2 (en) | 2010-04-28 | 2011-11-03 | Bayer Cropscience Ag | Fungicide hydroximoyl-tetrazole derivatives |
JP2013525400A (en) | 2010-04-28 | 2013-06-20 | バイエル・クロップサイエンス・アーゲー | Fungicide hydroxymoyl-heterocyclic derivative |
JP2013529074A (en) | 2010-05-04 | 2013-07-18 | ビーエーエスエフ ソシエタス・ヨーロピア | Plants with increased resistance to herbicides |
WO2011138280A2 (en) | 2010-05-04 | 2011-11-10 | Bayer Cropscience Ag | Herbicide/safener combinations comprising arylpyridazinones and safener |
AU2011256838B2 (en) | 2010-05-17 | 2014-10-09 | Sangamo Therapeutics, Inc. | Novel DNA-binding proteins and uses thereof |
US20110287933A1 (en) | 2010-05-21 | 2011-11-24 | Bayer Cropscience Ag | Herbicidal composition for tolerant or resistant oilseed rape crops |
EP2571364A1 (en) | 2010-05-21 | 2013-03-27 | Bayer Intellectual Property GmbH | Herbicidal agents for tolerant or resistant rice cultures |
CN103025166A (en) | 2010-05-21 | 2013-04-03 | 拜耳知识产权有限责任公司 | Herbicidal agents for tolerant or resistant grain cultures |
CN103025168A (en) | 2010-05-21 | 2013-04-03 | 拜耳知识产权有限责任公司 | Herbicidal agents for tolerant or resistant corn cultures |
CN103025161A (en) | 2010-05-24 | 2013-04-03 | 明治制果药业株式会社 | Noxious organism control agent |
MX344585B (en) | 2010-05-28 | 2016-12-20 | Nunhems Bv | Plants with increased fruit size. |
TWI584733B (en) | 2010-05-28 | 2017-06-01 | 巴地斯顏料化工廠 | Pesticidal mixture comprising abamectin, its use and methods of using the mixture |
TWI501727B (en) | 2010-05-28 | 2015-10-01 | Basf Se | Pesticidal mixtures |
CA2800369C (en) | 2010-05-31 | 2018-07-10 | Basf Se | Method for increasing the health of a plant |
JP5730992B2 (en) | 2010-06-03 | 2015-06-10 | バイエル・クロップサイエンス・アーゲーBayer Cropscience Ag | N-[(Heta) arylethyl)] pyrazole (thio) carboxamides and their hetero-substituted analogues |
ES2532971T3 (en) | 2010-06-03 | 2015-04-06 | Bayer Intellectual Property Gmbh | N - [(het) arylalkyl)] pyrazole (thio) carboxamides and their hetero substituted analogs |
UA110703C2 (en) | 2010-06-03 | 2016-02-10 | Байєр Кропсайнс Аг | Fungicidal n-[(trisubstitutedsilyl)methyl]carboxamide |
EP2580336B1 (en) | 2010-06-09 | 2017-05-10 | Bayer CropScience NV | Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering |
AU2011264075B2 (en) | 2010-06-09 | 2015-01-29 | Bayer Cropscience Nv | Methods and means to modify a plant genome at a nucleotide sequence commonly used in plant genome engineering |
BR112012031981A2 (en) | 2010-06-16 | 2015-09-15 | Basf Se | aqueous composition of active ingredient, process, use, method for controlling phytopathogenic fungi and seed |
WO2011161131A1 (en) | 2010-06-25 | 2011-12-29 | Basf Se | Herbicidal mixtures |
GB201010740D0 (en) | 2010-06-25 | 2010-08-11 | Univ Warwick | A plant |
WO2011161132A1 (en) | 2010-06-25 | 2011-12-29 | Basf Se | Pesticidal mixtures |
BR112012033668A2 (en) | 2010-07-01 | 2017-06-13 | Du Pont | transgenic plant and seed, method for producing a transgenic plant, product and / or by-product obtained from the transgenic seed, isolated polynucleotide and plant or seed comprising a recombinant DNA construct |
BR112013000434A2 (en) | 2010-07-07 | 2016-05-17 | Dow Agrosciences Llc | Linear DNA molecule delivery using pegylated quantum dots for stable transformation in plants. |
AU2011276127B2 (en) | 2010-07-08 | 2015-03-19 | University Of Copenhagen | Glucosinolate transporter protein and uses thereof |
WO2012007426A1 (en) | 2010-07-13 | 2012-01-19 | Basf Se | Azoline substituted isoxazoline benzamide compounds for combating animal pests |
WO2012010579A2 (en) | 2010-07-20 | 2012-01-26 | Bayer Cropscience Ag | Benzocycloalkenes as antifungal agents |
KR101911761B1 (en) | 2010-07-21 | 2018-10-25 | 바이엘 인텔렉쳐 프로퍼티 게엠베하 | (4-Halogenalkyl-3-thiobenzoyl)cyclohexanediones and use thereof as herbicides |
WO2012010574A1 (en) | 2010-07-21 | 2012-01-26 | Bayer Cropscience Ag | (4-trifluormethyl-3-thiobenzoyl)cyclohexanediones and use thereof as herbicides |
EP2595963B1 (en) | 2010-07-21 | 2014-11-12 | Bayer Intellectual Property GmbH | 4-(4-halogenalkyl-3-thiobenzoyl)pyrazoles and use thereof as herbicides |
BR112013003135A2 (en) | 2010-08-13 | 2017-11-07 | Pioneer Hi Bred Int | isolated or recombinant polynucleotide and polypeptide, nucleic acid construct, cell, plant, plant explant, transgenic seed, plant cell production method for weed control and detection of an hppd polypeptide and a polynucleotide. |
DE102011080568A1 (en) | 2010-08-16 | 2012-02-16 | Basf Se | New substituted cyanobutyrate compounds useful for combating weeds in culture plants e.g. cotton, rice, maize or wheat |
WO2012022729A2 (en) | 2010-08-20 | 2012-02-23 | Basf Se | Method for improving the health of a plant |
US9222100B2 (en) | 2010-08-24 | 2015-12-29 | Monsanto Technology Llc | Methods and DNA constructs for autoregulating transgene silencing |
CA2805770A1 (en) | 2010-08-24 | 2012-03-01 | Basf Se | Agrochemical mixtures for increasing the health of a plant |
JP5990170B2 (en) | 2010-09-01 | 2016-09-07 | バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングBayer Intellectual Property GmbH | Ketosultams and diketopyridines with herbicidal activity |
BR112013005070B1 (en) | 2010-09-01 | 2018-04-03 | Bayer Intellectual Property Gmbh | N- (TETRAZOL-5-IL) - AND N- (TRIAZOL-5-IL) ARYLARBOXAMIDES COMPOSITIONS, HERBICIDE COMPOSITION, ITS USES AS HERBICIDES AND METHOD TO CONTROL UNDESIRED PLANTS |
CN103189382A (en) | 2010-09-01 | 2013-07-03 | 拜耳知识产权有限责任公司 | Herbicide-effective pyridyl ketosultams |
PL2611300T3 (en) | 2010-09-03 | 2016-10-31 | Substituted annelated dihydropyrimidinone compounds | |
BR112013005382B1 (en) | 2010-09-13 | 2020-02-18 | Basf Se | USE OF A 3-PYRIDILLA COMPOUND, METHOD FOR CONTROLLING INVERTEBRATED PLACES, METHOD FOR PROTECTING VEGETABLE PROPAGATION MATERIAL AND / OR PLANTS AND AGRICULTURAL COMPOSITION |
BR112013005869A2 (en) | 2010-09-13 | 2019-09-24 | Basf Se | '' Method for controlling invertebrate pests, use of a compost, method, plant propagation material and agricultural composition '' |
WO2012034960A1 (en) | 2010-09-13 | 2012-03-22 | Basf Se | Pyridine compounds for controlling invertebrate pests ii |
AU2011303970B2 (en) | 2010-09-14 | 2015-08-13 | Basf Se | Composition containing a pyripyropene insecticide and an adjuvant |
CN103118537B (en) | 2010-09-14 | 2015-08-12 | 巴斯夫欧洲公司 | Composition pesticide containing pyripyropene insecticide and alkali |
EP2460406A1 (en) | 2010-12-01 | 2012-06-06 | Bayer CropScience AG | Use of fluopyram for controlling nematodes in nematode resistant crops |
BR112013006612A2 (en) | 2010-09-22 | 2017-10-24 | Bayer Ip Gmbh | use of biological or chemical control agents for insect and nematode control in resistant crops |
JP5905011B2 (en) | 2010-09-22 | 2016-04-20 | ブリティッシュ アメリカン タバコ (インヴェストメンツ) リミテッドBritish American Tobacco (Investments) Limited | Transformed plant |
JP2013540113A (en) | 2010-10-01 | 2013-10-31 | ビーエーエスエフ ソシエタス・ヨーロピア | Herbicidal benzoxazinone |
US20130184320A1 (en) | 2010-10-01 | 2013-07-18 | Basf Se | Imine Compounds |
CN103228627A (en) | 2010-10-01 | 2013-07-31 | 巴斯夫欧洲公司 | Imine substituted 2, 4 -diaryl - pyrroline derivatives as pesticides |
CN103237447A (en) | 2010-10-07 | 2013-08-07 | 巴斯夫欧洲公司 | Use of strobilurins for increasing the gluten strength in winter cereals |
CN103338638B (en) | 2010-10-07 | 2016-04-06 | 拜尔农科股份公司 | Comprise the fungicide composite of tetrazole radical 9 oxime derivate and Thiazolylpiperidine derivatives |
EP2443923A1 (en) | 2010-10-25 | 2012-04-25 | Basf Se | Composition comprising a pesticide and polycarboxylate ether |
JP2013540765A (en) | 2010-10-11 | 2013-11-07 | ビーエーエスエフ ソシエタス・ヨーロピア | Composition comprising a pesticide and a polycarboxylate ether |
UA107865C2 (en) | 2010-10-21 | 2015-02-25 | Байєр Інтелекчуал Проперті Гмбх | Heterocyclic carboxamides |
KR20130132816A (en) | 2010-10-21 | 2013-12-05 | 바이엘 인텔렉쳐 프로퍼티 게엠베하 | 1-(heterocyclic carbonyl) piperidines |
CA2815272A1 (en) | 2010-10-22 | 2012-04-26 | Bayer Intellectual Property Gmbh | Novel substituted picolinic acids, salts and acid derivatives thereof, and use thereof as herbicides |
CN103339096A (en) | 2010-11-02 | 2013-10-02 | 拜耳知识产权有限责任公司 | Phenyl-substituted bicyclooctane-1,3-dione-derivatives |
MX2013004878A (en) | 2010-11-02 | 2013-07-02 | Bayer Ip Gmbh | N-hetarylmethyl pyrazolylcarboxamides. |
MX2013005258A (en) | 2010-11-15 | 2013-07-05 | Bayer Ip Gmbh | N-aryl pyrazole(thio)carboxamides. |
CN107266368A (en) | 2010-11-15 | 2017-10-20 | 拜耳知识产权有限责任公司 | 5 halo-pyrazole formamides |
EP2640191A1 (en) | 2010-11-15 | 2013-09-25 | Bayer Intellectual Property GmbH | 5-halogenopyrazole(thio)carboxamides |
AU2010364322C1 (en) | 2010-11-24 | 2013-09-19 | E. I. Du Pont De Nemours And Company | Brassica GAT event DP-073496-4 and compositions and methods for the identification and/or detection thereof |
WO2012071039A1 (en) | 2010-11-24 | 2012-05-31 | Pioner Hi-Bred International, Inc. | Brassica gat event dp-061061-7 and compositions and methods for the identification and/or detection thereof |
EP2645856A1 (en) | 2010-12-01 | 2013-10-09 | Bayer Intellectual Property GmbH | Use of fluopyram for controlling nematodes in crops and for increasing yield |
EP2460407A1 (en) | 2010-12-01 | 2012-06-06 | Bayer CropScience AG | Agent combinations comprising pyridylethyl benzamides and other agents |
WO2012074868A2 (en) | 2010-12-03 | 2012-06-07 | Ms Technologies, Llc | Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells |
AR083029A1 (en) | 2010-12-09 | 2013-01-23 | Syngenta Participations Ag | METHODS AND COMPOSITIONS THAT USE SMALL INTERFERENT ARN (ARNIP) FOR THE CONTROL OF NEMATODES IN PLANTS |
WO2012076704A2 (en) | 2010-12-10 | 2012-06-14 | Basf Se | Pyrazole compounds for controlling invertebrate pests |
TWI667347B (en) | 2010-12-15 | 2019-08-01 | 瑞士商先正達合夥公司 | Soybean event syht0h2 and compositions and methods for detection thereof |
CN103261424B (en) | 2010-12-16 | 2017-12-12 | 巴斯夫农业公司 | There is the plant of the tolerance of enhancing to herbicide |
KR20140037804A (en) | 2010-12-16 | 2014-03-27 | 바이엘 인텔렉쳐 프로퍼티 게엠베하 | 6-(2-aminophenyl)picolinates and their use as herbicides |
AU2011347752A1 (en) | 2010-12-20 | 2013-07-11 | Basf Se | Pesticidal active mixtures comprising pyrazole compounds |
US20130274104A1 (en) | 2010-12-22 | 2013-10-17 | Basf Se | Agrochemical mixtures for increasing the health of a plant |
WO2012085081A1 (en) | 2010-12-22 | 2012-06-28 | Basf Se | Sulfoximinamide compounds for combating invertebrate pests ii |
EP2471776A1 (en) | 2010-12-28 | 2012-07-04 | Bayer CropScience AG | Pyridin-2-ylpropandinitriles and their use as herbicides |
EP2658853A1 (en) | 2010-12-29 | 2013-11-06 | Bayer Intellectual Property GmbH | Fungicide hydroximoyl-tetrazole derivatives |
EP2474542A1 (en) | 2010-12-29 | 2012-07-11 | Bayer CropScience AG | Fungicide hydroximoyl-tetrazole derivatives |
US9603322B2 (en) | 2010-12-30 | 2017-03-28 | Agrigenetics, Inc. | Canola cultivars having high yield and stabilized fatty acid profiles |
US8563811B2 (en) | 2010-12-30 | 2013-10-22 | Agrigenetics, Inc. | Canola cultivar DN040845A |
US8558065B2 (en) | 2010-12-30 | 2013-10-15 | Agrigenetics, Inc. | Canola cultivar G31064 |
US8563810B2 (en) | 2010-12-30 | 2013-10-22 | Agrigenetics, Inc. | Canola cultivar DN040244A |
US8530726B2 (en) | 2010-12-30 | 2013-09-10 | Agrigenetics, Inc. | Canola cultivar G030994 |
EP2471363A1 (en) | 2010-12-30 | 2012-07-04 | Bayer CropScience AG | Use of aryl-, heteroaryl- and benzylsulfonamide carboxylic acids, -carboxylic acid esters, -carboxylic acid amides and -carbonitriles and/or its salts for increasing stress tolerance in plants |
US8558064B2 (en) | 2010-12-30 | 2013-10-15 | Agrigenetics, Inc. | Canola cultivar CL31613 |
BR112013018531A2 (en) | 2011-01-24 | 2016-10-18 | Bayer Cropscience Nv | use of rd29 promoter or fragments thereof for stress-inducible expression of cotton transgenes |
EP2481284A3 (en) | 2011-01-27 | 2012-10-17 | Basf Se | Pesticidal mixtures |
CA2827398A1 (en) | 2011-02-17 | 2012-08-23 | Bayer Intellectual Property Gmbh | Substituted 3-(biphenyl-3-yl)-8,8-difluoro-4-hydroxy-1-azaspiro[4.5]dec-3-en-2-ones for therapy |
EA022869B1 (en) | 2011-02-28 | 2016-03-31 | Басф Се | Composition comprising a pesticide, a surfactant and an alkoxylate of 2-propylheptylamine |
AU2012222517B2 (en) | 2011-03-01 | 2016-09-22 | Bayer Intellectual Property Gmbh | 2-acyloxy-pyrrolin-4-ones |
EP2494867A1 (en) | 2011-03-01 | 2012-09-05 | Bayer CropScience AG | Halogen-substituted compounds in combination with fungicides |
CA2823999C (en) | 2011-03-10 | 2020-03-24 | Bayer Intellectual Property Gmbh | Use of lipochito-oligosaccharide compounds for safeguarding seed safety of treated seeds |
BR112013023502A2 (en) | 2011-03-14 | 2016-08-02 | Bayer Ip Gmbh | compound (i), fungicidal composition, method for the control of crop phytopathogenic fungi, use of the compounds of formula (i) and process for producing the compositions |
CA2830090C (en) | 2011-03-15 | 2019-07-16 | Bayer Intellectual Property Gmbh | Herbicide safener compositions |
KR101856998B1 (en) | 2011-03-15 | 2018-05-14 | 바이엘 인텔렉쳐 프로퍼티 게엠베하 | N-(1,2,5-oxadiazol-3-yl)-, n-(tetrazol-5-yl)- and n-(triazol-5-yl)bicycloarylcarboxamides and their use as herbicides |
EP2686316B1 (en) | 2011-03-15 | 2015-04-22 | Bayer Intellectual Property GmbH | N-(1,2,5-oxadiazol-3-yl)pyridinecarboxamides and use thereof as herbicides |
US8648230B2 (en) | 2011-03-18 | 2014-02-11 | Ms Technologies, Llc | Regulatory regions preferentially expressing in non-pollen plant tissue |
EP2693878B8 (en) | 2011-03-18 | 2018-08-29 | Bayer CropScience Aktiengesellschaft | Substituted (3r,4r)-4-cyan-3,4-diphenylbutanoates, method for the production thereof and use thereof as herbicides and plant growth regulators |
JP2014516919A (en) | 2011-03-18 | 2014-07-17 | バイエル・インテレクチユアル・プロパテイー・ゲー・エム・ベー・ハー | Substituted 4-cyano-3- (2,6-difluorophenyl) -4-phenylbutanoic acid compounds, processes for their preparation and their use as herbicides and plant growth regulators |
EA026386B1 (en) | 2011-03-22 | 2017-04-28 | Байер Интеллектуэль Проперти Гмбх | N-(1,3,4-oxadiazol-2-yl)arylcarboxamide or a salt thereof and use thereof as herbicide |
PL2688405T3 (en) | 2011-03-23 | 2018-05-30 | Basf Se | Compositions containing polymeric, ionic compounds comprising imidazolium groups |
JP6067671B2 (en) | 2011-03-23 | 2017-01-25 | ダウ アグロサイエンシィズ エルエルシー | Quantum dot carrier peptide conjugates suitable for imaging and delivery applications in plants |
PL2691379T3 (en) | 2011-03-31 | 2017-05-31 | Bayer Intellectual Property Gmbh | Herbicidally and fungicidally active 3-phenylisoxazoline-5-carboxamides and 3-phenylisoxazoline-5-thioamides |
US9179680B2 (en) | 2011-04-06 | 2015-11-10 | Basf Se | Substituted pyrimidinium compounds for combating animal pests |
US8513487B2 (en) | 2011-04-07 | 2013-08-20 | Zenon LISIECZKO | Plants and seeds of spring canola variety ND-662c |
US8513494B2 (en) | 2011-04-08 | 2013-08-20 | Chunren Wu | Plants and seeds of spring canola variety SCV695971 |
JP2014512358A (en) | 2011-04-08 | 2014-05-22 | バイエル・インテレクチユアル・プロパテイー・ゲー・エム・ベー・ハー | Fungicide hydroxymoyl-tetrazole derivative |
AR085872A1 (en) | 2011-04-08 | 2013-10-30 | Basf Se | HETEROBICICLIC DERIVATIVES N-SUBSTITUTES USEFUL TO COMBAT PARASITES IN PLANTS AND / OR ANIMALS, COMPOSITIONS THAT CONTAIN THEM AND METHODS TO COMBAT SUCH PESTS |
AR085568A1 (en) | 2011-04-15 | 2013-10-09 | Bayer Cropscience Ag | 5- (BICYCLE [4.1.0] HEPT-3-EN-2-IL) -PENTA-2,4-DIENOS AND 5- (BICYCLE [4.1.0] HEPT-3-EN-2-IL) -PENT- 2-IN-4-INOS REPLACED AS ACTIVE PRINCIPLES AGAINST ABIOTIC STRESS OF PLANTS |
AR090010A1 (en) | 2011-04-15 | 2014-10-15 | Bayer Cropscience Ag | 5- (CICLOHEX-2-EN-1-IL) -PENTA-2,4-DIENOS AND 5- (CICLOHEX-2-EN-1-IL) -PENT-2-EN-4-INOS REPLACED AS ACTIVE PRINCIPLES AGAINST THE ABIOTIC STRESS OF PLANTS, USES AND TREATMENT METHODS |
EP2511255A1 (en) | 2011-04-15 | 2012-10-17 | Bayer CropScience AG | Substituted prop-2-in-1-ol and prop-2-en-1-ol derivatives |
UY34014A (en) | 2011-04-15 | 2012-11-30 | Dow Agrosciences Llc | SYNTHETIC GENES TO EXPRESS PROTEINS IN CORN CELLS, CONSTRUCTIONS, TRANSGENIC PLANTS, PEST CONTROL METHODS AND COMPOSITIONS |
AR085585A1 (en) | 2011-04-15 | 2013-10-09 | Bayer Cropscience Ag | VINIL- AND ALQUINILCICLOHEXANOLES SUBSTITUTED AS ACTIVE PRINCIPLES AGAINST STRIPS ABIOTIQUE OF PLANTS |
GB201106964D0 (en) | 2011-04-21 | 2011-06-08 | Rothamsted Res Ltd | A method |
PT2699563T (en) | 2011-04-21 | 2016-07-14 | Basf Se | Novel pesticidal pyrazole compounds |
HUE043158T2 (en) | 2011-04-22 | 2019-08-28 | Bayer Cropscience Ag | Active compound compositions comprising a (thio)carboxamide derivative and a fungicidal compound |
WO2012148835A1 (en) | 2011-04-29 | 2012-11-01 | Pioneer Hi-Bred International, Inc. | Down-regulation of a homeodomain-leucine zipper i-class homeobox gene for improved plant performance |
US8507761B2 (en) | 2011-05-05 | 2013-08-13 | Teresa Huskowska | Plants and seeds of spring canola variety SCV372145 |
US8513495B2 (en) | 2011-05-10 | 2013-08-20 | Dale Burns | Plants and seeds of spring canola variety SCV291489 |
EP2524602A1 (en) | 2011-05-20 | 2012-11-21 | Bayer CropScience AG | Herbicide agent for tolerant or resistant soya cultures |
WO2012165961A1 (en) | 2011-05-31 | 2012-12-06 | Keygene N.V. | Pest resistant plants |
WO2012168124A1 (en) | 2011-06-06 | 2012-12-13 | Bayer Cropscience Nv | Methods and means to modify a plant genome at a preselected site |
FR2976950A1 (en) | 2011-06-24 | 2012-12-28 | Genoplante Valor | OBTAINING PLANTS HAVING IMPROVED TOLERANCE TO WATER STRESS |
PE20141518A1 (en) | 2011-07-01 | 2014-11-17 | Monsanto Technology Llc | METHODS AND COMPOSITIONS FOR THE SELECTIVE REGULATION OF PROTEIN EXPRESSION |
JP2014520776A (en) | 2011-07-04 | 2014-08-25 | バイエル・インテレクチユアル・プロパテイー・ゲー・エム・ベー・ハー | Use of substituted isoquinolinones, isoquinoline diones, isoquinoline triones and dihydroisoquinolinones or their salts in each case as active agents against abiotic stresses in plants |
EP2731933B1 (en) | 2011-07-15 | 2015-09-30 | Bayer Intellectual Property GmbH | 2,3-diphenyl-valeronitrile derivatives, method for the production thereof and use thereof as herbicides and plant growth regulators |
WO2013010946A2 (en) | 2011-07-15 | 2013-01-24 | Basf Se | Pesticidal methods using substituted 3-pyridyl thiazole compounds and derivatives for combating animal pests i |
US9179676B2 (en) | 2011-07-27 | 2015-11-10 | Bayer Intellectual Property Gmbh | Substituted picolinic acids and pyrimidine-4-carboxylic acids, method for the production thereof and use thereof as herbicides and plant growth regulators |
DE102011079991A1 (en) | 2011-07-28 | 2012-09-13 | Bayer Crop Science Ag | Use of seed treating-agent comprising nicotinoid insecticide as a safener for avoiding or reducing phytotoxic effects of herbicide on useful plants, preferably crop plants |
EP2486795A1 (en) | 2011-07-28 | 2012-08-15 | Bayer Cropscience AG | Use of seed treatment agents from the nicotinoid insecticide group as safeners for oxadiozole herbicides |
DE102011079997A1 (en) | 2011-07-28 | 2012-09-13 | Bayer Corpscience Ag | Use of seed treatment agents comprising pyrazole insecticides e.g. as safeners for avoiding or reducing phytotoxic effects of herbicides e.g. carbamate, thiocarbamate and haloacetanilide, on crops, preferably cultural crops |
EP2486797A1 (en) | 2011-07-28 | 2012-08-15 | Bayer CropScience AG | Use of seed treatment agents from the carbamate insecticide group as safeners for oxadiozole herbicides |
DE102011080001A1 (en) | 2011-07-28 | 2012-10-25 | Bayer Cropscience Ag | Use of seed treatment active substance comprising carbamate insecticides, e.g. as safeners for avoiding or reducing phytotoxic effects of herbicides on useful plants, preferably crop plants, and in crop plants protective agents |
DE102011080016A1 (en) | 2011-07-28 | 2012-10-25 | Bayer Cropscience Ag | Use of seed treatment active substance comprising strobilurin fungicides, e.g. as safeners for avoiding or reducing phytotoxic effects of herbicides on useful plants, preferably crop plants, and in crop plants protective agents |
EP2486796A1 (en) | 2011-07-28 | 2012-08-15 | Bayer CropScience AG | Use of seed treatment agents from the pyrazole insecticide group as safeners for oxadiozole herbicides |
DE102011080020A1 (en) | 2011-07-28 | 2012-09-13 | Bayer Cropscience Ag | Use of seed treatment agents, comprising dicarboximide fungicides as safeners, for preventing or reducing phytotoxic effects of herbicides on useful plants, preferably cultivated plants |
DE102011080010A1 (en) | 2011-07-28 | 2012-10-25 | Bayer Cropscience Ag | Use of seed treatment agents comprising anilide and thiazole fungicides, e.g. as safeners for avoiding or reducing phytotoxic effects of herbicides e.g. carbamate, thiocarbamate and haloacetanilide, on crops, preferably cultural crops |
DE102011080004A1 (en) | 2011-07-28 | 2012-09-13 | Bayer Cropscience Ag | Use of seed treatment agents, comprising carbamate fungicides as safeners, for preventing or reducing phytotoxic effects of herbicides on useful plants, preferably cultivated plants |
DE102011080007A1 (en) | 2011-07-28 | 2012-09-13 | Bayer Cropscience Ag | Use of seed treatment agents comprising conazole or triazole fungicides e.g. as safeners for avoiding or reducing phytotoxic effects of herbicides e.g. carbamate, thiocarbamate and haloacetanilide, on crops, preferably cultural crops |
US8822378B2 (en) | 2011-08-03 | 2014-09-02 | Bayer Intellectual Property Gmbh | N-(tetrazol-5-yl)- and N-(triazol-5-yl)arylcarboxamides and use thereof as herbicides |
US8785729B2 (en) | 2011-08-09 | 2014-07-22 | Nunhems, B.V. | Lettuce variety redglace |
US9265252B2 (en) | 2011-08-10 | 2016-02-23 | Bayer Intellectual Property Gmbh | Active compound combinations comprising specific tetramic acid derivatives |
JP6042433B2 (en) | 2011-08-11 | 2016-12-14 | バイエル・インテレクチュアル・プロパティ・ゲゼルシャフト・ミット・ベシュレンクテル・ハフツングBayer Intellectual Property GmbH | 1,2,4-Triazolyl-substituted ketoenols |
IN2014CN01024A (en) | 2011-08-12 | 2015-04-10 | Basf Se | |
CA2843083A1 (en) | 2011-08-12 | 2013-02-21 | Basf Se | Anthranilamide compounds and their use as pesticides |
JP2014522876A (en) | 2011-08-12 | 2014-09-08 | ビーエーエスエフ ソシエタス・ヨーロピア | N-thio-anthranilamide compounds and their use as pesticides |
EP2742037B1 (en) | 2011-08-12 | 2015-10-14 | Basf Se | N-thio-anthranilamide compounds and their use as pesticides |
CN103717612A (en) | 2011-08-12 | 2014-04-09 | 拜尔作物科学公司 | Guard cell-specific expression of transgenes in cotton |
BR112014002970A2 (en) | 2011-08-12 | 2017-02-21 | Basf Se | compost, method for preparing a compost, agricultural or veterinary composition, method for combating or controlling invertebrate pests, method for protecting plant cultivation, method for protecting seeds, seed, uses of compost and method for treating |
WO2013024003A1 (en) | 2011-08-12 | 2013-02-21 | Basf Se | N-thio-anthranilamide compounds and their use as pesticides |
EP2742021A1 (en) | 2011-08-12 | 2014-06-18 | Basf Se | Aniline type compounds |
CN103889960A (en) | 2011-08-18 | 2014-06-25 | 巴斯夫欧洲公司 | Carbamoylmethoxy- and carbamoylmethylthio- and carbamoylmethylamino benzamides for combating invertebrate pests |
US20140243196A1 (en) | 2011-08-18 | 2014-08-28 | Basf Se | Carbamoylmethoxy- and Carbamoylmethylthio- and Carbamoylmethylamino Benzamides for Combating Invertebrate Pests |
JP2014524434A (en) | 2011-08-18 | 2014-09-22 | ビーエーエスエフ ソシエタス・ヨーロピア | Carbamoylmethoxybenzamide and carbamoylmethylthiobenzamide and carbamoylmethylaminobenzamide for combating harmful invertebrates |
EP2748161A1 (en) | 2011-08-22 | 2014-07-02 | Bayer Intellectual Property GmbH | Fungicide hydroximoyl-tetrazole derivatives |
BR122014004140B8 (en) | 2011-08-22 | 2023-03-28 | Bayer Cropscience Ag | RECOMBINANT VECTOR OR RECOMBINANT CONSTRUCTION, AS WELL AS METHODS FOR OBTAINING AND PRODUCING A COTTON PLANT OR PLANT CELL TOLERANT TO AN HPPD INHIBITOR, AND FOR CULTIVATING A FIELD OF COTTON PLANTS |
EP2561759A1 (en) | 2011-08-26 | 2013-02-27 | Bayer Cropscience AG | Fluoroalkyl-substituted 2-amidobenzimidazoles and their effect on plant growth |
IN2014CN02367A (en) | 2011-09-02 | 2015-06-19 | Basf Se | |
CN104023535A (en) | 2011-09-02 | 2014-09-03 | 巴斯夫欧洲公司 | Use of pesticidal active 3-arylquinazolin-4-one derivatives in soil application methods |
JP2014525424A (en) | 2011-09-02 | 2014-09-29 | ビーエーエスエフ ソシエタス・ヨーロピア | Agricultural mixture containing arylquinazolinone compounds |
US8754293B2 (en) | 2011-09-09 | 2014-06-17 | Nunhems B.V. | Lettuce variety intred |
WO2013034621A1 (en) | 2011-09-09 | 2013-03-14 | Bayer Intellectual Property Gmbh | Acyl-homoserine lactone derivatives for improving plant yield |
CN103874681B (en) | 2011-09-12 | 2017-01-18 | 拜耳知识产权有限责任公司 | Fungicidal 4-substituted-3-{phenyl[(heterocyclylmethoxy)imino]methyl}-1,2,4-oxadizol-5(4H)-one derivatives |
CA2848689A1 (en) | 2011-09-13 | 2013-03-21 | Monsanto Technology Llc | Methods and compositions for weed control targeting pds |
US9840715B1 (en) | 2011-09-13 | 2017-12-12 | Monsanto Technology Llc | Methods and compositions for delaying senescence and improving disease tolerance and yield in plants |
MX362812B (en) | 2011-09-13 | 2019-02-13 | Monsanto Technology Llc | Methods and compositions for weed control. |
US10829828B2 (en) | 2011-09-13 | 2020-11-10 | Monsanto Technology Llc | Methods and compositions for weed control |
WO2013040005A1 (en) | 2011-09-13 | 2013-03-21 | Monsanto Technology Llc | Methods and compositions for weed control |
MX343072B (en) | 2011-09-13 | 2016-10-21 | Monsanto Technology Llc | Methods and compositions for weed control. |
US10760086B2 (en) | 2011-09-13 | 2020-09-01 | Monsanto Technology Llc | Methods and compositions for weed control |
US10806146B2 (en) | 2011-09-13 | 2020-10-20 | Monsanto Technology Llc | Methods and compositions for weed control |
UA116092C2 (en) | 2011-09-13 | 2018-02-12 | Монсанто Текнолоджи Ллс | Methods and compositions for weed control |
AU2012308686B2 (en) | 2011-09-13 | 2018-05-10 | Monsanto Technology Llc | Methods and compositions for weed control |
EP2756085B1 (en) | 2011-09-13 | 2019-03-20 | Monsanto Technology LLC | Methods and compositions for weed control |
EP2756083B1 (en) | 2011-09-13 | 2020-08-19 | Monsanto Technology LLC | Methods and compositions for weed control |
US9920326B1 (en) | 2011-09-14 | 2018-03-20 | Monsanto Technology Llc | Methods and compositions for increasing invertase activity in plants |
AR087874A1 (en) | 2011-09-16 | 2014-04-23 | Bayer Ip Gmbh | USE OF ACILSULPHONAMIDES TO IMPROVE THE PERFORMANCE OF PLANTS |
WO2013037956A1 (en) | 2011-09-16 | 2013-03-21 | Bayer Intellectual Property Gmbh | Use of 5-phenyl- or 5-benzyl-2 isoxazoline-3 carboxylates for improving plant yield |
BR112014005990B1 (en) | 2011-09-16 | 2019-12-31 | Bayer Ip Gmbh | method for inducing a specific plant growth regulating response |
EP2757886A1 (en) | 2011-09-23 | 2014-07-30 | Bayer Intellectual Property GmbH | Use of 4-substituted 1-phenyl-pyrazole-3-carboxylic-acid derivatives as agents against abiotic plant stress |
EP2764101B1 (en) | 2011-10-04 | 2017-03-29 | Bayer Intellectual Property GmbH | RNAi FOR THE CONTROL OF FUNGI AND OOMYCETES BY INHIBITING SACCHAROPINE DEHYDROGENASE GENE |
WO2013050324A1 (en) | 2011-10-06 | 2013-04-11 | Bayer Intellectual Property Gmbh | Combination, containing 4-phenylbutyric acid (4-pba) or a salt thereof (component (a)) and one or more selected additional agronomically active compounds (component(s) (b)), that reduces abiotic plant stress |
US20140259223A1 (en) | 2011-10-19 | 2014-09-11 | Keygene N.V. | Methods for producing cinnamolide and/or drimendiol |
EA025426B1 (en) | 2011-10-31 | 2016-12-30 | Байер Интеллектчуал Проперти Гмбх | Substituted 4-cyano-3-phenyl-4-(pyridin-3-yl)butanoates, processes for preparation thereof and use thereof as herbicides and plant growth regulators |
HUP1400505A2 (en) | 2011-11-03 | 2015-03-02 | Syngenta Participations Ag | Polynucleotides, polypeptides and methods for enhancing photossimilation in plants |
EP2589293A1 (en) | 2011-11-03 | 2013-05-08 | Bayer CropScience AG | Herbicide safener compounds containing N-(Tetrazol-5-yl)- and N-(Triazol-5-yl)aryl carboxylic acid amides |
US8957096B2 (en) | 2011-11-03 | 2015-02-17 | Bayer Intellectual Property Gmbh | Herbicidally active oxime-ether-substituted benzoylamides |
EP2589598A1 (en) | 2011-11-03 | 2013-05-08 | Bayer CropScience AG | 5-phenyl substituted N-(Tetrazol-5-yl)- and N-(Triazol-5-yl)aryl carboxylic acid amides and use of same as herbicides |
SI2776038T1 (en) | 2011-11-11 | 2018-06-29 | Gilead Apollo, Llc | Acc inhibitors and uses thereof |
US20140323306A1 (en) | 2011-11-14 | 2014-10-30 | Basf Se | Substituted 1,2,5-Oxadiazole Compounds and Their Use as Herbicides |
MX2014005607A (en) | 2011-11-16 | 2014-07-30 | Basf Se | Substituted 1,2,5-oxadiazole compounds and their use as herbicides ii. |
CN104039780A (en) | 2011-11-18 | 2014-09-10 | 巴斯夫欧洲公司 | Substituted 1,2,5-oxadiazole compounds and their use as herbicides III |
US9210857B1 (en) | 2011-11-21 | 2015-12-15 | Agrigenetics, Inc. | Canola inbred CL102407R |
US9204602B1 (en) | 2011-11-21 | 2015-12-08 | Agrigenetics, Inc. | Canola inbred CL77606R |
US9204601B1 (en) | 2011-11-21 | 2015-12-08 | Agrigenetics, Inc. | Canola inbred CL60855R |
EP2782920B1 (en) | 2011-11-21 | 2016-12-21 | Bayer Intellectual Property GmbH | Fungicide n-[(trisubstitutedsilyl)methyl]-carboxamide derivatives |
CN105906567B (en) | 2011-11-30 | 2019-01-22 | 拜耳知识产权有限责任公司 | Antifungal N- bicyclic alkyl and N- tricyclic alkyl (thio) carboxamide derivative |
UA116532C2 (en) | 2011-12-13 | 2018-04-10 | Байєр Інтеллектуал Проперті Гмбх | N-(1,2,5-oxadiazol-3-yl)-, n-(1,3,4-oxadiazol-2-yl)-, n-(tetrazol-5-yl)- und n-(triazol-5-yl)-arylcarbonsaureamide und ihre verwendung als herbizide |
WO2013095125A1 (en) | 2011-12-16 | 2013-06-27 | Keygene N.V. | Method for producing a plant having enhanced disease resistance to nematodes |
AR089249A1 (en) | 2011-12-19 | 2014-08-06 | Bayer Ip Gmbh | 4-CIANO-3-PHENYL-4- (PIRIDIN-3-IL) SUBSTITUTED BUTANOATS, PROCEDURES FOR THEIR PREPARATION AND ITS USE AS HERBICIDES AND AS REGULATORS OF GROWTH OF PLANTS |
AU2012357896B9 (en) | 2011-12-19 | 2016-12-15 | Bayer Cropscience Ag | Use of anthranilic acid diamide derivatives for pest control in transgenic crops |
US20130167262A1 (en) | 2011-12-21 | 2013-06-27 | The Curators Of The University Of Missouri | Soybean variety s05-11268 |
CN104023724A (en) | 2011-12-21 | 2014-09-03 | 巴斯夫欧洲公司 | N-thio-anthranilamide compounds and their use as pesticides |
US9204603B2 (en) | 2011-12-21 | 2015-12-08 | The Curators Of The University Of Missouri | Soybean variety S05-11482 |
EP2794601B1 (en) | 2011-12-23 | 2019-02-20 | Basf Se | Isothiazoline compounds for combating invertebrate pests |
KR102028903B1 (en) | 2011-12-29 | 2019-10-07 | 바이엘 인텔렉쳐 프로퍼티 게엠베하 | Fungicidal 3-[(pyridin-2-ylmethoxyimino)(phenyl)methyl]-2-substituted-1,2,4-oxadiazol-5(2h)-one derivatives |
CN104039769B (en) | 2011-12-29 | 2016-10-19 | 拜耳知识产权有限责任公司 | 3-[(1,3-thiazole-4-yl methoxyimino) (phenyl) methyl]-2-substituted-1,2,4-diazole-5 (2H) the-one derivant of antifungal |
US9380756B2 (en) | 2012-01-04 | 2016-07-05 | Nunhems B.V. | Lettuce variety multigreen 50 |
US9006515B2 (en) | 2012-01-06 | 2015-04-14 | Pioneer Hi Bred International Inc | Pollen preferred promoters and methods of use |
BR112014016791A2 (en) | 2012-01-06 | 2019-09-24 | Pioneer Hi Bred Int | isolated nucleic acid molecule, expression cassette, vector, plant cell, plant, transgenic seed, method for expression of a polynucleotide in a plant or plant cell, method for expression of a polynucleotide, preferably in egg tissues of a plant |
WO2013104705A1 (en) | 2012-01-11 | 2013-07-18 | Bayer Intellectual Property Gmbh | Tetrazol-5-yl- and triazol-5-yl-aryl compounds and use thereof as herbicides |
US9499831B2 (en) | 2012-01-17 | 2016-11-22 | Pioneer Hi-Bred International, Inc. | Plant transcription factors, promoters and uses thereof |
CN104219950B (en) | 2012-02-01 | 2018-02-06 | 陶氏益农公司 | Chloroplast transit peptides derived from artificial Btassica |
WO2013113789A1 (en) | 2012-02-02 | 2013-08-08 | Basf Se | N-thio-anthranilamide compounds and their use as pesticides |
BR112014020164B1 (en) | 2012-02-16 | 2022-09-13 | Syngenta Participations Ag | MANIPULATED PESTICIDE VIP3 POLYPEPTIDE, NUCLEIC ACID MOLECULE, VECTOR AND COMPOSITION |
US9156784B2 (en) | 2012-02-21 | 2015-10-13 | Bayer Intellectual Property Gmbh | Herbicidal sulfinimidoyl- and sulfonimidoyl benzoyl derivatives |
US9169219B2 (en) | 2012-02-21 | 2015-10-27 | Bayer Intellectual Property Gmbh | Herbicidally active 4-nitro-substituted N-(tetrazol-5-yl)-, N-(triazol-5-yl)-, and N-(1,3,4-0XADIAZOL-2-yl)aryl carboxylic acid amides |
WO2013124228A1 (en) | 2012-02-21 | 2013-08-29 | Bayer Intellectual Property Gmbh | Herbicidal 3 - ( sulfin- /sulfonimidoyl) - benzamides |
US9198425B2 (en) | 2012-02-21 | 2015-12-01 | Bayer Intellectual Property Gmbh | Herbicidally-effective sulfinyl aminobenzamides |
WO2013124246A1 (en) | 2012-02-22 | 2013-08-29 | Bayer Intellectual Property Gmbh | Herbicidally active 4-dialkoxymethyl-2-phenylpyrimidines |
PT2816897T (en) | 2012-02-22 | 2018-04-02 | Bayer Cropscience Ag | Use of fluopyram for controlling wood diseases in grape |
UA113198C2 (en) | 2012-02-27 | 2016-12-26 | COMBINATIONS OF ACTIVE COMPOUNDS | |
CA2865571A1 (en) | 2012-02-29 | 2013-09-06 | Bayer Cropscience Nv | Als inhibitor herbicide tolerant b. napus mutants |
BR112014021199A2 (en) | 2012-03-01 | 2018-05-08 | Basf Se | composition, method for preparing composition, polymer, method for preparing polymer, method for controlling fungi and seed |
MX360700B (en) | 2012-03-12 | 2018-11-14 | Basf Se | Liquid concentrate formulation containing a pyripyropene insecticide ii. |
KR102056608B1 (en) | 2012-03-12 | 2019-12-17 | 바스프 에스이 | Method for producing an aqueous suspension concentrate formulation of a pyripyropene insecticide |
US9596843B2 (en) | 2012-03-12 | 2017-03-21 | Basf Se | Liquid concentrate formulation containing a pyripyropene insecticide I |
US20150203864A1 (en) | 2012-03-13 | 2015-07-23 | University Of Guelph | Myb55 promoter and use thereof |
US20150031535A1 (en) | 2012-03-13 | 2015-01-29 | Basf Se | Liquid concentrate formulation containing a pyripyropene insecticide III |
WO2013138309A1 (en) | 2012-03-13 | 2013-09-19 | Pioneer Hi-Bred International, Inc. | Genetic reduction of male fertility in plants |
EA201491670A1 (en) | 2012-03-13 | 2015-07-30 | Пайонир Хай-Бред Интернэшнл, Инк. | GENETIC REDUCTION OF MALE REPRODUCTIVE FUNCTION IN PLANTS |
GB201204862D0 (en) | 2012-03-20 | 2012-05-02 | Cambridge Advanced Tech | Transgenic plants |
GB201204871D0 (en) | 2012-03-20 | 2012-05-02 | Cambridge Advanced Tech | Transgenic plants |
GB201204869D0 (en) | 2012-03-20 | 2012-05-02 | Cambridge Advanced Tech | Transgenic plants |
WO2013139949A1 (en) | 2012-03-23 | 2013-09-26 | Bayer Intellectual Property Gmbh | Compositions comprising a strigolactame compound for enhanced plant growth and yield |
WO2013143927A1 (en) | 2012-03-29 | 2013-10-03 | Basf Se | Co-crystals of dicamba and a co-crystal former b |
CN104203925A (en) | 2012-03-29 | 2014-12-10 | 拜耳知识产权有限责任公司 | 5-aminopyrimidine derivatives and use thereof for combating undesired plant growth |
WO2013144228A1 (en) | 2012-03-29 | 2013-10-03 | Basf Se | Pesticidal methods using heterocyclic compounds and derivatives for combating animal pests |
WO2013144223A1 (en) | 2012-03-30 | 2013-10-03 | Basf Se | N-substituted pyrimidinylidene compounds and derivatives for combating animal pests |
ES2626360T3 (en) | 2012-03-30 | 2017-07-24 | Basf Se | N-substituted thiocarbonyl pyridinylidene compounds and their use to combat animal pests |
US20150065343A1 (en) | 2012-04-02 | 2015-03-05 | Basf Se | Acrylamide compounds for combating invertebrate pests |
WO2013149903A1 (en) | 2012-04-03 | 2013-10-10 | Basf Se | N- substituted hetero - bicyclic furanone derivatives for combating animal |
WO2013150115A1 (en) | 2012-04-05 | 2013-10-10 | Basf Se | N- substituted hetero - bicyclic compounds and derivatives for combating animal pests |
US9357778B2 (en) | 2012-04-12 | 2016-06-07 | Bayer Cropscience Ag | N-acyl-2-(cyclo)alkypyrrolidines and piperidines useful as fungicides |
KR102062517B1 (en) | 2012-04-20 | 2020-01-06 | 바이엘 크롭사이언스 악티엔게젤샤프트 | N-cycloalkyl-n-[(heterocyclylphenyl)methylene]-(thio)carboxamide derivatives |
JP2015516396A (en) | 2012-04-20 | 2015-06-11 | バイエル・クロップサイエンス・アーゲーBayer Cropscience Ag | N-cycloalkyl-N-[(trisubstituted silylphenyl) methylene]-(thio) carboxamide derivatives |
WO2013160230A1 (en) | 2012-04-23 | 2013-10-31 | Bayer Cropscience Nv | Targeted genome engineering in plants |
US8835720B2 (en) | 2012-04-26 | 2014-09-16 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV967592 |
US8802935B2 (en) | 2012-04-26 | 2014-08-12 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV942568 |
US8878009B2 (en) | 2012-04-26 | 2014-11-04 | Monsanto Technology, LLP | Plants and seeds of spring canola variety SCV318181 |
US8859857B2 (en) | 2012-04-26 | 2014-10-14 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV259778 |
US20150291570A1 (en) | 2012-04-27 | 2015-10-15 | Basf Se | Substituted N-(tetrazol-5-yl)- and N-(triazol-5-yl)arylcarboxamide compounds and their use as herbicides |
JP2015519316A (en) | 2012-04-27 | 2015-07-09 | ビーエーエスエフ ソシエタス・ヨーロピアBasf Se | Substituted N- (tetrazol-5-yl)-and N- (triazol-5-yl) hetarylcarboxamide compounds and their use as herbicides |
BR112014026789B1 (en) | 2012-04-27 | 2019-10-22 | Basf Se | n- (tetrazol-5-yl) and n- (triazol-5-yl) aryl carboxamides compounds, composition, use of a compound and method for controlling unwanted vegetation |
IN2014MN02211A (en) | 2012-04-27 | 2015-07-10 | Basf Se | |
EA025517B1 (en) | 2012-05-03 | 2016-12-30 | Байер Кропсайенс Аг | N-(tetrazol-5-yl)- and n-(triazol-5-yl)arylcarboxamide salts and use thereof as herbicides |
EA201401213A1 (en) | 2012-05-04 | 2015-04-30 | Басф Се | SUBSTITUTED PYRAZOL-CONTAINING COMPOUNDS AND THEIR APPLICATION AS PESTICIDES |
EP2662364A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazole tetrahydronaphthyl carboxamides |
EP2662370A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole benzofuranyl carboxamides |
EP2662360A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole indanyl carboxamides |
EP2662362A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazole indanyl carboxamides |
BR112014027133A2 (en) | 2012-05-09 | 2017-06-27 | Basf Se | compound, agricultural or veterinary composition, method for controlling invertebrate pests, plant propagating material and method for treating or protecting an animal. |
EP2662361A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | Pyrazol indanyl carboxamides |
EP2847170B1 (en) | 2012-05-09 | 2017-11-08 | Bayer CropScience AG | Pyrazole indanyl carboxamides |
EP2662363A1 (en) | 2012-05-09 | 2013-11-13 | Bayer CropScience AG | 5-Halogenopyrazole biphenylcarboxamides |
BR112014027644A2 (en) | 2012-05-09 | 2017-06-27 | Bayer Cropscience Ag | 5-halopyrazole indanyl carboxamides |
AR091104A1 (en) | 2012-05-22 | 2015-01-14 | Bayer Cropscience Ag | COMBINATIONS OF ACTIVE COMPOUNDS THAT INCLUDE A LIPO-CHYTOOLIGOSACARIDE DERIVATIVE AND A NEMATICIDE, INSECTICIDE OR FUNGICIDE COMPOUND |
US9512113B2 (en) | 2012-05-24 | 2016-12-06 | Bayer Cropscience Ag | N-(tetrazol-5-yl)- and N-(triazol-5-yl)arylcarboxylic thioamides and use thereof as herbicides |
IN2014MN02404A (en) | 2012-05-24 | 2015-08-21 | Seeds Ltd Ab | |
BR112014028882B1 (en) | 2012-05-24 | 2019-09-03 | Bayer Cropscience Ag | herbicidal composition and method for weed control in crops |
CN104487439B (en) | 2012-05-24 | 2017-06-09 | 巴斯夫欧洲公司 | N Thio-anthranilamids compound and its purposes as insecticide |
CN104540951B (en) | 2012-06-07 | 2017-07-04 | 美国陶氏益农公司 | Use the construct and method of the two-way constitutive promoter express transgenic of Btassica |
WO2013186089A2 (en) | 2012-06-14 | 2013-12-19 | Basf Se | Pesticidal methods using substituted 3-pyridyl thiazole compounds and derivatives for combating animal pests |
CA2876426A1 (en) | 2012-06-15 | 2013-12-19 | E. I. Du Pont De Nemours And Company | Methods and compositions involving als variants with native substrate preference |
CA2877290A1 (en) | 2012-06-19 | 2013-12-27 | Daniel F. Voytas | Gene targeting in plants using dna viruses |
AR091489A1 (en) | 2012-06-19 | 2015-02-11 | Basf Se | PLANTS THAT HAVE A GREATER TOLERANCE TO HERBICIDES INHIBITORS OF PROTOPORFIRINOGENO OXIDASA (PPO) |
EP3646731A1 (en) | 2012-06-20 | 2020-05-06 | Basf Se | Pesticidal mixtures comprising a pyrazole compound |
WO2013189777A1 (en) | 2012-06-21 | 2013-12-27 | Basf Se | Adjuvant comprising a 2-propylheptylamine alkoxylate, sugar-based surfactant, and drift-control agent and/or humectant |
WO2014001248A1 (en) | 2012-06-27 | 2014-01-03 | Bayer Cropscience Ag | Herbicidal compositions comprising flufenacet |
WO2014001361A1 (en) | 2012-06-27 | 2014-01-03 | Bayer Cropscience Ag | Herbicidal agents containing flufenacet |
HUE042995T2 (en) | 2012-06-27 | 2019-07-29 | Bayer Cropscience Ag | Herbicidal agents containing flufenacet |
WO2014001357A1 (en) | 2012-06-27 | 2014-01-03 | Bayer Cropscience Ag | Herbicidal agents containing flufenacet |
US10119147B2 (en) | 2012-07-06 | 2018-11-06 | Washington State University | Brassica plants with modified seed oil composition |
EP2684879A1 (en) | 2012-07-09 | 2014-01-15 | Basf Se | Substituted mesoionic compounds for combating animal pests |
EP2871958A1 (en) | 2012-07-11 | 2015-05-20 | Bayer CropScience AG | Use of fungicidal combinations for increasing the tolerance of a plant towards abiotic stress |
CA2882143A1 (en) | 2012-07-26 | 2014-01-30 | Dow Agrosciences Llc | High-throughput dna fragment assembly |
AU2012208997B1 (en) | 2012-07-30 | 2013-09-19 | Dlf Usa Inc. | An alfalfa variety named magnum salt |
CA3116898C (en) | 2012-08-17 | 2022-09-27 | Dow Agrosciences Llc | Use of a maize untranslated region for transgene expression in plants |
BR112015004648A2 (en) | 2012-09-05 | 2017-07-04 | Bayer Cropscience Ag | bicycloaryl carboxylic acid amides with herbicidal activity |
EA201590482A1 (en) | 2012-09-05 | 2015-07-30 | Байер Кропсайенс Аг | APPLICATION OF SUBSTITUTED 2-AMIDOBENZIMIDAZOLES, 2-AMIDOBENZOXAZOLES AND 2-AMIDOBENZOTHIAZOLES OR THEIR SALTS AS A BIOLOGICALLY ACTIVE SUBSTANCE AGAINST THE ABIOTIC STRESS RESISTANCE |
UA118090C2 (en) | 2012-09-07 | 2018-11-26 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks |
CA2884162C (en) | 2012-09-07 | 2020-12-29 | Dow Agrosciences Llc | Fad3 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks |
BR112015006299A2 (en) | 2012-09-21 | 2017-07-04 | Basf Se | '' compost, agricultural composition, method for the protection of crop plants, method for the protection of plant propagating material and propagating material '' |
CN104797564B (en) | 2012-09-25 | 2018-05-04 | 拜尔农作物科学股份公司 | The 3- phenyl-isoxazole oxazoline -5- thioamides of the 3- phenyl-isoxazoles oxazoline -5- formamides and 5- epoxides of weeding and antifungal 5- epoxides-substituted-substituted |
WO2014053403A1 (en) | 2012-10-01 | 2014-04-10 | Basf Se | Method of controlling insecticide resistant insects |
WO2014053401A2 (en) | 2012-10-01 | 2014-04-10 | Basf Se | Method of improving plant health |
CN104768377A (en) | 2012-10-01 | 2015-07-08 | 巴斯夫欧洲公司 | Pesticidally active mixtures comprising anthranilamide compounds |
WO2014053407A1 (en) | 2012-10-01 | 2014-04-10 | Basf Se | N-thio-anthranilamide compounds and their use as pesticides |
WO2014053406A1 (en) | 2012-10-01 | 2014-04-10 | Basf Se | Method of controlling ryanodine-modulator insecticide resistant insects |
AR094139A1 (en) | 2012-10-01 | 2015-07-15 | Basf Se | ACTIVE MIXTURES AS PESTICIDES, WHICH INCLUDE ANTRANILAMIDE COMPOUNDS |
JP2015532274A (en) | 2012-10-01 | 2015-11-09 | ビーエーエスエフ ソシエタス・ヨーロピアBasf Se | Use of N-thio-anthranilamide compounds in cultivated plants |
JP6182215B2 (en) | 2012-10-04 | 2017-08-16 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | 1,2,4-triazine-3,5-dione-6-carboxamide and its use as a herbicide |
WO2014059155A1 (en) | 2012-10-11 | 2014-04-17 | Pioneer Hi-Bred International, Inc. | Guard cell promoters and uses thereof |
BR112015008706A2 (en) | 2012-10-18 | 2018-02-06 | Monsanto Technology Llc | methods and compositions for plant pest control |
JP6153619B2 (en) | 2012-10-19 | 2017-06-28 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | Combinations of active compounds including carboxamide derivatives |
MX2015004773A (en) | 2012-10-19 | 2015-08-14 | Bayer Cropscience Ag | Method of plant growth promotion using carboxamide derivatives. |
MX2015004778A (en) | 2012-10-19 | 2015-08-14 | Bayer Cropscience Ag | Method for enhancing tolerance to abiotic stress in plants using carboxamide or thiocarboxamide derivatives. |
MX363731B (en) | 2012-10-19 | 2019-04-01 | Bayer Cropscience Ag | Method for treating plants against fungi resistant to fungicides using carboxamide or thiocarboxamide derivatives. |
EP2914094B1 (en) | 2012-11-01 | 2021-07-21 | Cellectis | Plants for production of therapeutic proteins |
UA117816C2 (en) | 2012-11-06 | 2018-10-10 | Байєр Кропсайєнс Акцієнгезелльшафт | Herbicidal combinations for tolerant soybean cultures |
WO2014079820A1 (en) | 2012-11-22 | 2014-05-30 | Basf Se | Use of anthranilamide compounds for reducing insect-vectored viral infections |
WO2014079957A1 (en) | 2012-11-23 | 2014-05-30 | Bayer Cropscience Ag | Selective inhibition of ethylene signal transduction |
EP2735231A1 (en) | 2012-11-23 | 2014-05-28 | Bayer CropScience AG | Active compound combinations |
US20150307894A1 (en) | 2012-11-28 | 2015-10-29 | Monsanto Technology Llc | Transgenic Plants With Enhanced Traits |
US9447430B1 (en) | 2012-11-29 | 2016-09-20 | Agrigenetics, Inc. | Canola inbred line G2X0023AB |
US9445564B1 (en) | 2012-11-29 | 2016-09-20 | Agrigenetics, Inc. | Canola inbred line DN051465A |
US9414556B1 (en) | 2012-11-29 | 2016-08-16 | Agrigenetics, Inc. | Canola inbred restorer line G98014R |
WO2014083089A1 (en) | 2012-11-30 | 2014-06-05 | Bayer Cropscience Ag | Ternary fungicidal and pesticidal mixtures |
MX2015006327A (en) | 2012-11-30 | 2015-10-05 | Bayer Cropscience Ag | Ternary fungicidal mixtures. |
BR112015012473A2 (en) | 2012-11-30 | 2017-07-11 | Bayer Cropscience Ag | pesticide and fungicide binary mixtures |
EA201500580A1 (en) | 2012-11-30 | 2016-01-29 | Байер Кропсайенс Акциенгезельшафт | DOUBLE FUNGICIDE MIXTURES |
JP6367214B2 (en) | 2012-11-30 | 2018-08-01 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | Two-component fungicide mixture or two-component pesticide mixture |
EP2740720A1 (en) | 2012-12-05 | 2014-06-11 | Bayer CropScience AG | Substituted bicyclic and tricyclic pent-2-en-4-inic acid derivatives and their use for enhancing the stress tolerance in plants |
BR112015013084A2 (en) | 2012-12-05 | 2017-09-26 | British American Tobacco Investments Ltd | method of modulating lignin density in a plant, use of a genetic construct, use of a transgenic plant, tobacco product, smoking article |
EP2740356A1 (en) | 2012-12-05 | 2014-06-11 | Bayer CropScience AG | Substituted (2Z)-5(1-Hydroxycyclohexyl)pent-2-en-4-inic acid derivatives |
BR112015012926A2 (en) | 2012-12-05 | 2017-07-11 | Bayer Cropscience Ag | use of 1- (aryl ethinyl) -, 1- (heteroaryl ethinyl) -, 1- (heterocyclyl ethinyl) substituted and 1- (cycloalkenyl ethinyl) cyclohexanols as active agents against abiotic plant stress |
EP2928887B1 (en) | 2012-12-06 | 2016-11-16 | Bayer CropScience AG | N-(oxazol-2-yl)aryl carboxylic acid amides and use of same as herbicides |
WO2014086737A1 (en) | 2012-12-06 | 2014-06-12 | Bayer Cropscience Ag | Condensed 2-pyridone-3-carboxamides and the use thereof as herbicides |
AR093909A1 (en) | 2012-12-12 | 2015-06-24 | Bayer Cropscience Ag | USE OF ACTIVE INGREDIENTS TO CONTROL NEMATODES IN CULTURES RESISTANT TO NEMATODES |
US20140173775A1 (en) | 2012-12-13 | 2014-06-19 | Pioneer Hi-Bred International, Inc. | Methods and compositions for producing and selecting transgenic plants |
JP2016501264A (en) | 2012-12-14 | 2016-01-18 | ビーエーエスエフ ソシエタス・ヨーロピアBasf Se | Malononitrile compounds for controlling animal pests |
AR094006A1 (en) | 2012-12-18 | 2015-07-01 | Bayer Cropscience Ag | HERBICIDE AGENTS CONTAINING ACLONIFEN |
AR093996A1 (en) | 2012-12-18 | 2015-07-01 | Bayer Cropscience Ag | BACTERICIDAL COMBINATIONS AND BINARY FUNGICIDES |
AR093998A1 (en) | 2012-12-18 | 2015-07-01 | Bayer Cropscience Ag | HERBICIDE AGENTS CONTAINING ACLONIFEN |
AR093997A1 (en) | 2012-12-18 | 2015-07-01 | Bayer Cropscience Ag | HERBICIDE AGENTS CONTAINING ACLONIFEN |
US9428459B2 (en) | 2012-12-19 | 2016-08-30 | Bayer Cropscience Ag | Difluoromethyl-nicotinic- tetrahydronaphtyl carboxamides |
AU2013361220A1 (en) | 2012-12-21 | 2015-04-02 | Pioneer Hi-Bred International, Inc. | Compositions and methods for auxin-analog conjugation |
JP2016505585A (en) | 2012-12-21 | 2016-02-25 | ビーエーエスエフ ソシエタス・ヨーロピアBasf Se | Cycloclabine and its derivatives for controlling invertebrate pests |
US20140178561A1 (en) | 2012-12-21 | 2014-06-26 | Cellectis | Potatoes with reduced cold-induced sweetening |
US20150368236A1 (en) | 2012-12-27 | 2015-12-24 | Basf Se | 2-(pyridin-3-yl)-5-hetaryl-thiazole compounds carrying an imine or imine-derived substituent for combating invertebrate pests |
CN105358695B (en) | 2013-01-01 | 2019-07-12 | A.B.种子有限公司 | Method by dsRNA introduced plant seed to adjust gene expression |
US10683505B2 (en) | 2013-01-01 | 2020-06-16 | Monsanto Technology Llc | Methods of introducing dsRNA to plant seeds for modulating gene expression |
US20140203176A1 (en) | 2013-01-23 | 2014-07-24 | Dow Agrosciences Llc | Systems and methods for real-time sampling and analysis of biomolecules beneath the surface of biological tissue |
US10000767B2 (en) | 2013-01-28 | 2018-06-19 | Monsanto Technology Llc | Methods and compositions for plant pest control |
WO2014128136A1 (en) | 2013-02-20 | 2014-08-28 | Basf Se | Anthranilamide compounds and their use as pesticides |
US9538716B1 (en) | 2013-02-21 | 2017-01-10 | Agrigenetics, Inc. | Canola inbred restorer line CE185942R |
US9426953B1 (en) | 2013-02-21 | 2016-08-30 | Agrigenetics, Inc. | Canola hybrid cultivar CE216910H |
US9414557B1 (en) | 2013-02-21 | 2016-08-16 | Agrigenetics, Inc. | Canola inbred restorer line CE185952R |
US20140245475A1 (en) | 2013-02-27 | 2014-08-28 | Bayer Cropscience Lp | Cotton variety st 4946glb2 |
EP2964033A1 (en) | 2013-03-07 | 2016-01-13 | Basf Se | Co-crystals of pyrimethanil and selected dithiine tetracarboximide |
WO2014135608A1 (en) | 2013-03-07 | 2014-09-12 | Bayer Cropscience Ag | Fungicidal 3-{phenyl[(heterocyclylmethoxy)imino]methyl}-heterocycle derivatives |
WO2014159306A1 (en) | 2013-03-13 | 2014-10-02 | Pioneer Hi-Bred International, Inc. | Glyphosate application for weed control in brassica |
CA2905027A1 (en) | 2013-03-13 | 2014-10-09 | Monsanto Technology Llc | Methods and compositions for weed control |
EP2967082A4 (en) | 2013-03-13 | 2016-11-02 | Monsanto Technology Llc | Methods and compositions for weed control |
US20160017360A1 (en) | 2013-03-13 | 2016-01-21 | Pioneer Hi-Bred International, Inc. | Functional expression of bacterial major facilitator superfamily mfs gene in maize to improve agronomic traits and grain yield |
US20160010101A1 (en) | 2013-03-13 | 2016-01-14 | Pioneer Hi-Bred International, Inc. | Enhanced nitrate uptake and nitrate translocation by over- expressing maize functional low-affinity nitrate transporters in transgenic maize |
US20140283211A1 (en) | 2013-03-14 | 2014-09-18 | Monsanto Technology Llc | Methods and Compositions for Plant Pest Control |
US20140304857A1 (en) | 2013-03-14 | 2014-10-09 | Pioneer Hi Bred International Inc | Maize stress related transcription factor 18 and uses thereof |
BR112015023286A2 (en) | 2013-03-14 | 2018-03-06 | Arzeda Corp | recombinant polypeptide with dicamba decarboxylase activity, polynucleotide construct, cell, method of producing a host cell comprising a heterologous polynucleotide encoding a dicamba decarboxylase activity, method for decarboxylating dicamba, a dicamba derivative or a dicamba metabolite, method for detecting a polypeptide and method for detecting the presence of a polynucleotide encoding a polypeptide having dicamba decarboxylase activity |
EP2970363B1 (en) | 2013-03-14 | 2020-07-08 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
WO2014142647A1 (en) | 2013-03-14 | 2014-09-18 | Wageningen Universiteit | Fungals strains with improved citric acid and itaconic acid production |
US20160053277A1 (en) | 2013-03-14 | 2016-02-25 | Pioneer Hi-Bred International, Inc. | Compositions Having Dicamba Decarboxylase Activity and Methods of Use |
US10568328B2 (en) | 2013-03-15 | 2020-02-25 | Monsanto Technology Llc | Methods and compositions for weed control |
US10023877B2 (en) | 2013-03-15 | 2018-07-17 | Pioneer Hi-Bred International, Inc. | PHI-4 polypeptides and methods for their use |
US10113162B2 (en) | 2013-03-15 | 2018-10-30 | Cellectis | Modifying soybean oil composition through targeted knockout of the FAD2-1A/1B genes |
WO2014143996A2 (en) | 2013-03-15 | 2014-09-18 | Pioneer Hi-Bred International, Inc. | Compositions and methods of use of acc oxidase polynucleotides and polypeptides |
US20160053274A1 (en) | 2013-04-02 | 2016-02-25 | Bayer Cropscience Nv | Targeted genome engineering in eukaryotes |
WO2014161908A1 (en) | 2013-04-05 | 2014-10-09 | Bayer Cropscience Nv | Brassica plants comprising mutant da1 alleles |
JP2016522800A (en) | 2013-04-12 | 2016-08-04 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | New triazoline thione derivatives |
MX2015014365A (en) | 2013-04-12 | 2015-12-07 | Bayer Cropscience Ag | Novel triazole derivatives. |
EP2986117A1 (en) | 2013-04-19 | 2016-02-24 | Bayer CropScience Aktiengesellschaft | Binary insecticidal or pesticidal mixture |
CA2909725A1 (en) | 2013-04-19 | 2014-10-23 | Bayer Cropscience Aktiengesellschaft | Method for improved utilization of the production potential of transgenic plants |
US20160050923A1 (en) | 2013-04-19 | 2016-02-25 | Basf Se | N-substituted acyl-imino-pyridine compounds and derivatives for combating animal pests |
WO2014177514A1 (en) | 2013-04-30 | 2014-11-06 | Bayer Cropscience Ag | Nematicidal n-substituted phenethylcarboxamides |
TW201507722A (en) | 2013-04-30 | 2015-03-01 | Bayer Cropscience Ag | N-(2-halogen-2-phenethyl)carboxamides as nematicides and endoparasiticides |
CA2911818A1 (en) | 2013-05-10 | 2014-11-13 | Nimbus Apollo, Inc. | Acc inhibitors and uses thereof |
WO2014184019A1 (en) | 2013-05-15 | 2014-11-20 | Basf Se | N-(1,2,5-oxadiazol-3-yl)carboxamide compounds and their use as herbicides |
BR112015028597A2 (en) | 2013-05-15 | 2017-07-25 | Basf Se | n- (tetrazol-5-yl) - and n- (5-triazol-yl) arylcarboxamides, composition, use of a compound and method for controlling vegetation |
WO2014184014A1 (en) | 2013-05-15 | 2014-11-20 | Basf Se | N-(1,2,5-oxadiazol-3-yl)carboxamide compounds and their use as herbicides |
WO2014184058A1 (en) | 2013-05-15 | 2014-11-20 | Basf Se | Substituted 1,2,5-oxadiazole compounds and their use as herbicides |
AR096517A1 (en) | 2013-06-07 | 2016-01-13 | Bayer Cropscience Ag | DERIVATIVES OF 5-HIDROXI-2,3-DIFENYLPENTANONITRILE REPLACED, PROCEDURES FOR THEIR PREPARATION AND ITS USE AS HERBICIDES AND / OR REGULATORS OF GROWTH OF PLANTS |
AU2014278519B2 (en) | 2013-06-11 | 2020-09-10 | Syngenta Participations Ag | Methods for generating transgenic plants |
DE102013010026A1 (en) | 2013-06-17 | 2014-12-18 | Kws Saat Ag | Resistance gene against Rizomania |
EP2815649A1 (en) | 2013-06-18 | 2014-12-24 | Basf Se | Fungicidal mixtures II comprising strobilurin-type fungicides |
EP3013802B1 (en) | 2013-06-26 | 2019-08-14 | Bayer Cropscience AG | N-cycloalkyl-n-[(bicyclylphenyl)methylene]-(thio)carboxamide derivatives |
AU2014289341A1 (en) | 2013-07-09 | 2016-01-28 | Bayer Cropscience Aktiengesellschaft | Use of selected pyridone carboxamides or salts thereof as active substances against abiotic plant stress |
WO2015004242A1 (en) | 2013-07-12 | 2015-01-15 | Bayer Cropscience Nv | Als inhibitor herbicide tolerant mutant plants |
AR097138A1 (en) | 2013-07-15 | 2016-02-24 | Basf Se | PESTICIDED COMPOUNDS |
CN105377836B (en) | 2013-07-18 | 2018-04-10 | 巴斯夫欧洲公司 | Substituted N (base of 1,2,4 triazole 3) aryl carboxamide compounds and its purposes as herbicide |
MX359191B (en) | 2013-07-19 | 2018-09-18 | Monsanto Technology Llc | Compositions and methods for controlling leptinotarsa. |
US9850496B2 (en) | 2013-07-19 | 2017-12-26 | Monsanto Technology Llc | Compositions and methods for controlling Leptinotarsa |
WO2015013509A1 (en) | 2013-07-25 | 2015-01-29 | Pioneer Hi-Bred International, Inc. | Method for producing hybrid brassica seed |
EP2835052A1 (en) | 2013-08-07 | 2015-02-11 | Basf Se | Fungicidal mixtures comprising pyrimidine fungicides |
EP2837287A1 (en) | 2013-08-15 | 2015-02-18 | Bayer CropScience AG | Use of prothioconazole for increasing root growth of Brassicaceae |
AR097362A1 (en) | 2013-08-16 | 2016-03-09 | Cheminova As | COMBINATION OF 2-METHYLBYPHENYL-3-ILLAMETABLE (Z) - (1R) -CIS-3- (2-CHLORINE-3,3,3-TRIFLUORPROP-1-ENIL) -2, 2-DIMETHYLCYCLOPROPANOCARBOXYLATE WITH AT LEAST ONE INSECTICIDE , ACARICIDE, NEMATICIDE AND / OR FUNGICIDE |
EP3032942B1 (en) | 2013-08-16 | 2020-03-11 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
EP3043635B1 (en) | 2013-09-13 | 2020-02-12 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CA2922506A1 (en) | 2013-09-19 | 2015-03-26 | Basf Se | N-acylimino heterocyclic compounds |
EP3049517B1 (en) | 2013-09-24 | 2018-04-11 | Bayer CropScience NV | Hetero-transglycosylase and uses thereof |
WO2015054106A1 (en) | 2013-10-07 | 2015-04-16 | Monsanto Technology Llc | Transgenic plants with enhanced traits |
WO2015052178A1 (en) | 2013-10-10 | 2015-04-16 | Basf Se | 1,2,5-oxadiazole compounds and their use as herbicides |
CN105636950A (en) | 2013-10-10 | 2016-06-01 | 巴斯夫欧洲公司 | Substituted n-(tetrazol-5-yl)- and n-(triazol-5-yl)arylcarboxamide compounds and their use as herbicides |
WO2015052173A1 (en) | 2013-10-10 | 2015-04-16 | Basf Se | Tetrazole and triazole compounds and their use as herbicides |
US10329578B2 (en) | 2013-10-18 | 2019-06-25 | Pioneer Hi-Bred International, Inc. | Glyphosate-N-acetyltransferase (GLYAT) sequences and methods of use |
WO2015055757A1 (en) | 2013-10-18 | 2015-04-23 | Basf Se | Use of pesticidal active carboxamide derivative in soil and seed application and treatment methods |
WO2015061548A1 (en) | 2013-10-25 | 2015-04-30 | Pioneer Hi-Bred International, Inc. | Stem canker tolerant soybeans and methods of use |
JP6452687B2 (en) | 2013-10-25 | 2019-01-16 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | Herbicidal composition containing N- (1,3,4-oxadiazol-2-yl) -arylcarboxylic amides |
EP3066109A4 (en) | 2013-11-04 | 2017-11-29 | Dow AgroSciences LLC | Optimal soybean loci |
AU2014341927B2 (en) | 2013-11-04 | 2017-12-14 | Corteva Agriscience Llc | Optimal maize loci |
CA2928666C (en) | 2013-11-04 | 2023-05-23 | Dow Agrosciences Llc | Optimal maize loci for targeted genome modification |
MX2016005778A (en) | 2013-11-04 | 2016-12-20 | Monsanto Technology Llc | Compositions and methods for controlling arthropod parasite and pest infestations. |
MX358066B (en) | 2013-11-04 | 2018-08-03 | Dow Agrosciences Llc | Optimal soybean loci. |
EP2868197A1 (en) | 2013-11-05 | 2015-05-06 | Basf Se | Herbicidal compositions |
EP2868196A1 (en) | 2013-11-05 | 2015-05-06 | Basf Se | Herbicidal compositions |
EP3068772A1 (en) | 2013-11-15 | 2016-09-21 | Bayer CropScience Aktiengesellschaft | 2-hetaryl-pyridazinone derivatives and their use as herbicides |
CA2932484A1 (en) | 2013-12-05 | 2015-06-11 | Bayer Cropscience Aktiengesellschaft | N-cycloalkyl-n-{[2-(1-substitutedcycloalkyl)phenyl]methylene}-(thio)carboxamide derivatives |
WO2015082587A1 (en) | 2013-12-05 | 2015-06-11 | Bayer Cropscience Ag | N-cycloalkyl-n-{[2-(1-substitutedcycloalkyl)phenyl]methylene}-(thio)carboxamide derivatives |
CN111793640A (en) | 2013-12-06 | 2020-10-20 | 奥驰亚客户服务公司 | Tobacco plants and methods of using such plants |
UA119253C2 (en) | 2013-12-10 | 2019-05-27 | Біолоджикс, Інк. | Compositions and methods for virus control in varroa mite and bees |
RU2659002C1 (en) | 2013-12-10 | 2018-06-26 | ДАУ АГРОСАЙЕНСИЗ ЭлЭлСи | Synergistic protection against weeds using the herbicides and of agricultural culture improved stability with the use of combinations, including 2,4-d-choline and gluphosinate, in tolerant relative to 2,4-d-choline and gluphosinate soy, corn, cotton |
EP3083581A1 (en) | 2013-12-18 | 2016-10-26 | Basf Se | N-substituted imino heterocyclic compounds |
EA201691198A1 (en) | 2013-12-18 | 2016-11-30 | Басф Агро Б.В. | PLANTS OWNED BY ENHANCED TOLERANCE TO HERBICIDES |
US20160318897A1 (en) | 2013-12-18 | 2016-11-03 | Basf Se | Azole compounds carrying an imine-derived substituent |
WO2015094884A1 (en) | 2013-12-20 | 2015-06-25 | Dow Agrosciences Llc | Synergistic herbicidal weed control |
TW201527316A (en) | 2013-12-31 | 2015-07-16 | Dow Agrosciences Llc | Novel maize ubiquitin promoters |
TW201527314A (en) | 2013-12-31 | 2015-07-16 | Dow Agrosciences Llc | Novel maize ubiquitin promoters |
TW201527312A (en) | 2013-12-31 | 2015-07-16 | Dow Agrosciences Llc | Novel maize ubiquitin promoters |
US10683513B2 (en) | 2013-12-31 | 2020-06-16 | Dow Agrosciences Llc | Tissue-specific expression and hybrid plant production |
TW201527313A (en) | 2013-12-31 | 2015-07-16 | Dow Agrosciences Llc | Novel maize ubiquitin promoters |
WO2015104422A1 (en) | 2014-01-13 | 2015-07-16 | Basf Se | Dihydrothiophene compounds for controlling invertebrate pests |
AU2015206585A1 (en) | 2014-01-15 | 2016-07-21 | Monsanto Technology Llc | Methods and compositions for weed control using EPSPS polynucleotides |
AR100304A1 (en) | 2014-02-05 | 2016-09-28 | Basf Corp | SEED COATING FORMULATION |
EP3102684B1 (en) | 2014-02-07 | 2020-05-06 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
WO2015120276A1 (en) | 2014-02-07 | 2015-08-13 | Pioneer Hi Bred International Inc | Insecticidal proteins and methods for their use |
EP2907807A1 (en) | 2014-02-18 | 2015-08-19 | Basf Se | Benzamide compounds and their use as herbicides |
US9596816B1 (en) | 2014-02-28 | 2017-03-21 | Agrigenetics, Inc. | Canola inbred restorer line CL215695R |
TW201538518A (en) | 2014-02-28 | 2015-10-16 | Dow Agrosciences Llc | Root specific expression conferred by chimeric gene regulatory elements |
US9554534B1 (en) | 2014-02-28 | 2017-01-31 | Agrigenetics, Inc. | Canola inbred line CL1992625A |
US9844195B1 (en) | 2014-02-28 | 2017-12-19 | Agrigenetics, Inc. | Canola hybrid cultivar CL2537387H |
US9854763B1 (en) | 2014-02-28 | 2018-01-02 | Agrigenetics, Inc. | Canola inbred line CL1992625B |
EP2918582A1 (en) | 2014-03-11 | 2015-09-16 | Bayer CropScience AG | 5-(Azinedionyl)-pyridazinone derivatives and their use as herbicides |
EP2918581A1 (en) | 2014-03-11 | 2015-09-16 | Bayer CropScience AG | 2-(Azinedionyl)-pyridazinone derivatives and their use as herbicides |
EP3125676A4 (en) | 2014-04-01 | 2018-02-14 | Monsanto Technology LLC | Compositions and methods for controlling insect pests |
WO2015150465A2 (en) | 2014-04-03 | 2015-10-08 | Basf Se | Plants having increased tolerance to herbicides |
US9522936B2 (en) | 2014-04-24 | 2016-12-20 | Sangamo Biosciences, Inc. | Engineered transcription activator like effector (TALE) proteins |
RU2704450C2 (en) | 2014-05-06 | 2019-10-28 | Басф Се | Composition containing pesticide and hydroxyalkyl ether of polyoxyethylene glycol |
EP3145918B1 (en) | 2014-05-21 | 2018-01-03 | Bayer CropScience Aktiengesellschaft | 2-(hetero)aryl pyridazinones and their use as herbicides |
AR100448A1 (en) | 2014-05-21 | 2016-10-05 | Bayer Cropscience Ag | 5- (HETERO) ARIL-PIRIDAZINONAS AND ITS USE AS A HERBICIDE |
AR100785A1 (en) | 2014-06-09 | 2016-11-02 | Dow Agrosciences Llc | HERBICIDE CONTROL OF MALEZA FROM COMBINATIONS OF FLUROXIPIR AND INHIBITORS OF ALS |
CA2952906A1 (en) | 2014-06-20 | 2015-12-23 | Cellectis | Potatoes with reduced granule-bound starch synthase |
CA2953347A1 (en) | 2014-06-23 | 2015-12-30 | Monsanto Technology Llc | Compositions and methods for regulating gene expression via rna interference |
EA036537B1 (en) | 2014-06-25 | 2020-11-20 | Басф Агро Б.В. | Pesticidal compositions |
WO2015200539A1 (en) | 2014-06-25 | 2015-12-30 | Monsanto Technology Llc | Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression |
EP2962567A1 (en) | 2014-07-01 | 2016-01-06 | Basf Se | Ternary mixtures comprising biopesticides and at least two chemical insecticides |
US10113174B2 (en) | 2014-07-02 | 2018-10-30 | Altria Client Services Llc | Tobacco having altered leaf properties and methods of making and using |
US9686931B2 (en) | 2014-07-07 | 2017-06-27 | Alforex Seeds LLC | Hybrid alfalfa variety named HybriForce-3400 |
US10626409B2 (en) | 2014-07-08 | 2020-04-21 | Altria Client Services Llc | Genetic locus imparting a low anatabine trait in tobacco and methods of using |
UA120058C2 (en) | 2014-07-14 | 2019-09-25 | Басф Се | Pesticidal compositions |
AR101214A1 (en) | 2014-07-22 | 2016-11-30 | Bayer Cropscience Ag | CIANO-CICLOALQUILPENTA-2,4-DIENOS, CIANO-CICLOALQUILPENT-2-EN-4-INAS, CIANO-HETEROCICLILPENTA-2,4-DIENOS AND CYANO-HETEROCICLILPENT-2-EN-4-INAS REPLACED AS ACTIVE PRINCIPLES PLANTS ABIOTIC |
EP3174982A4 (en) | 2014-07-29 | 2018-06-20 | Monsanto Technology LLC | Compositions and methods for controlling insect pests |
EP2979549A1 (en) | 2014-07-31 | 2016-02-03 | Basf Se | Method for improving the health of a plant |
CA2955828A1 (en) | 2014-08-08 | 2016-02-11 | Pioneer Hi-Bred International, Inc. | Ubiquitin promoters and introns and methods of use |
WO2016034615A1 (en) | 2014-09-02 | 2016-03-10 | BASF Agro B.V. | Aqueous insecticide formulation containing hyperbranched polymer |
WO2016044092A1 (en) | 2014-09-17 | 2016-03-24 | Pioneer Hi Bred International Inc | Compositions and methods to control insect pests |
WO2016049531A1 (en) | 2014-09-26 | 2016-03-31 | Purecircle Usa Inc. | Single nucleotide polymorphism (snp) markers for stevia |
CA2962242A1 (en) | 2014-09-29 | 2016-04-07 | Agrigenetics, Inc. | Low lignin non-transgenic alfalfa varieties and methods for producing the same |
CN113652416A (en) | 2014-10-06 | 2021-11-16 | 奥驰亚客户服务有限公司 | Genetic control of axillary bud growth in tobacco plants |
US10435706B2 (en) | 2014-10-16 | 2019-10-08 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
EP3227448A1 (en) | 2014-12-01 | 2017-10-11 | Basf Se | A method for screening of genes conferring increased tolerance to herbicides |
EP3028573A1 (en) | 2014-12-05 | 2016-06-08 | Basf Se | Use of a triazole fungicide on transgenic plants |
US10165751B2 (en) | 2014-12-05 | 2019-01-01 | Agrigenetics, Inc. | Canola inbred line G30853A |
US9986702B1 (en) | 2014-12-05 | 2018-06-05 | Agrigenetics, Inc. | Canola inbred restorer line G1934899R |
WO2016091674A1 (en) | 2014-12-12 | 2016-06-16 | Basf Se | Use of cyclaniliprole on cultivated plants |
US10537109B2 (en) | 2014-12-12 | 2020-01-21 | Syngenta Participations Ag | Compositions and methods for controlling plant pests |
WO2016091675A1 (en) | 2014-12-12 | 2016-06-16 | Basf Se | Method for improving the health of a plant |
AR103024A1 (en) | 2014-12-18 | 2017-04-12 | Bayer Cropscience Ag | SELECTED PYRIDONCARBOXAMIDS OR ITS SALTS AS ACTIVE SUBSTANCES AGAINST ABIOTIC PLANTS STRESS |
US20170359965A1 (en) | 2014-12-19 | 2017-12-21 | E I Du Pont De Nemours And Company | Polylactic acid compositions with accelerated degradation rate and increased heat stability |
US9743603B2 (en) | 2014-12-22 | 2017-08-29 | Monsanto Technology Llc | Cotton variety 14R925B2XF |
US9743605B2 (en) | 2014-12-22 | 2017-08-29 | Monsanto Technology Llc | Cotton variety 14R950B2XF |
US9788507B2 (en) | 2014-12-22 | 2017-10-17 | Monsanto Technology Llc | Cotton variety 14R955B2XF |
US9668447B2 (en) | 2014-12-22 | 2017-06-06 | Monsanto Technology Llc | Cotton variety 14R941B2XF |
US9723801B2 (en) | 2014-12-22 | 2017-08-08 | Monsanto Technology Llc | Cotton variety 14R952B2XF |
US9820451B2 (en) | 2014-12-22 | 2017-11-21 | Monsanto Technology Llc | Cotton variety 14R948B2XF |
US9615530B2 (en) | 2014-12-22 | 2017-04-11 | Monsanto Technology Llc | Cotton variety 14R938B2XF |
US9820452B2 (en) | 2014-12-22 | 2017-11-21 | Monsanto Technology Llc | Cotton variety 14R915B2XF |
US9717208B2 (en) | 2014-12-22 | 2017-08-01 | Monsanto Technology Llc | Cotton variety 14R953B2XF |
US9826691B2 (en) | 2014-12-22 | 2017-11-28 | Monsanto Technology Llc | Cotton variety 14R914B2XF |
US9820450B2 (en) | 2014-12-22 | 2017-11-21 | Monsanto Technology Llc | Cotton variety 14R942B2XF |
US9743602B2 (en) | 2014-12-22 | 2017-08-29 | Monsanto Technology Llc | Cotton variety 14R911B2XF |
US9743604B2 (en) | 2014-12-22 | 2017-08-29 | Monsanto Technology Llc | Cotton variety 14R949B2XF |
US9924654B2 (en) | 2014-12-22 | 2018-03-27 | Monsanto Technology Llc | Cotton variety 14R922B2XF |
WO2016113333A1 (en) | 2015-01-16 | 2016-07-21 | Bayer Cropscience Nv | Leaf-preferential promoters and uses thereof |
EP3247200A4 (en) | 2015-01-21 | 2018-06-13 | Basf Se | Plants having increased tolerance to herbicides |
RU2723049C2 (en) | 2015-01-22 | 2020-06-08 | Монсанто Текнолоджи Ллс | Compositions and methods for controlling leptinotarsa |
US10757935B2 (en) | 2015-02-10 | 2020-09-01 | Basf Se | Composition comprising a pesticide and an alkoxylated ester |
AR103649A1 (en) | 2015-02-11 | 2017-05-24 | Basf Se | HYDROXYPHENYL PYRUVATE DIOXYGENASES RESISTANT TO HERBICIDES |
WO2016128519A1 (en) | 2015-02-12 | 2016-08-18 | Bayer Cropscience Nv | Shoot apex-preferential promoters and uses thereof |
EP3061346A1 (en) | 2015-02-26 | 2016-08-31 | Bayer CropScience AG | Use of fluopyram and biological control agents to control harmful fungi |
US9854765B2 (en) | 2015-02-26 | 2018-01-02 | Monsanto Technology Llc | Cotton variety 14R934B2XF |
ES2761806T3 (en) | 2015-03-17 | 2020-05-21 | Bayer Cropscience Ag | Salts of n- (1,3,4-oxadiazol-2-yl) aryl carboxylic acid amides and their use as herbicides |
US9968047B2 (en) | 2015-03-24 | 2018-05-15 | Agrigenetics, Inc. | Canola hybrid cultivar CL2562968H |
AU2016239537B2 (en) | 2015-03-31 | 2020-02-06 | Basf Se | Composition comprising a pesticide and isononanoic acid N,N-dimethyl amide |
US10314270B2 (en) | 2015-04-01 | 2019-06-11 | Agrigenetics, Inc. | Canola hybrid cultivar G3697124H |
CA2980505A1 (en) | 2015-04-07 | 2016-10-13 | Basf Agrochemical Products B.V. | Use of an insecticidal carboxamide compound against pests on cultivated plants |
EP3283476B1 (en) | 2015-04-13 | 2019-08-14 | Bayer Cropscience AG | N-cycloalkyl-n-(biheterocyclyethylene)-(thio)carboxamide derivatives |
US9968050B2 (en) | 2015-04-15 | 2018-05-15 | Agrigenetics, Inc. | Canola inbred restorer line G175274R |
US10306852B2 (en) | 2015-04-15 | 2019-06-04 | Agrigenetics, Inc. | Canola inbred line G1992650A |
US9968051B2 (en) | 2015-04-15 | 2018-05-15 | Agrigenetics, Inc. | Canola hybrid cultivar G2537376H |
US9974262B2 (en) | 2015-04-15 | 2018-05-22 | Agrigenetics, Inc. | Canola inbred restorer line CL134904R |
WO2016174042A1 (en) | 2015-04-27 | 2016-11-03 | BASF Agro B.V. | Pesticidal compositions |
US10494642B2 (en) | 2015-04-28 | 2019-12-03 | Basf Agricultural Solutions Seed, Us Llc | Brassica plants with modified seed oil composition |
RU2017144238A (en) | 2015-05-19 | 2019-06-19 | Пайонир Хай-Бред Интернэшнл, Инк. | INSECTICIDAL PROTEINS AND METHODS OF THEIR APPLICATION |
UY36703A (en) | 2015-06-02 | 2016-12-30 | Monsanto Technology Llc | COMPOSITIONS AND METHODS FOR THE ADMINISTRATION OF A POLINUCLEOTIDE ON A PLANT |
CN108024517A (en) | 2015-06-03 | 2018-05-11 | 孟山都技术公司 | For the method and composition introducing nucleic acid in plant |
AU2016278142A1 (en) | 2015-06-16 | 2017-11-30 | E. I. Du Pont De Nemours And Company | Compositions and methods to control insect pests |
AU2016279062A1 (en) | 2015-06-18 | 2019-03-28 | Omar O. Abudayyeh | Novel CRISPR enzymes and systems |
EP3111763A1 (en) | 2015-07-02 | 2017-01-04 | BASF Agro B.V. | Pesticidal compositions comprising a triazole compound |
PL3316692T3 (en) | 2015-07-02 | 2021-10-11 | BASF Agro B.V. | Pesticidal compositions comprising a triazole compound |
ES2747753T3 (en) | 2015-07-03 | 2020-03-11 | Bayer Cropscience Ag | N- (1,3,4-Oxadiazol-2-yl) arylcarboxamide derivatives with herbicidal action |
JP6677749B2 (en) | 2015-07-03 | 2020-04-08 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | N- (tetrazol-5-yl) arylcarboxamide derivatives and N- (triazol-5-yl) arylcarboxamide derivatives having herbicidal action |
EP3118199A1 (en) | 2015-07-13 | 2017-01-18 | Bayer CropScience AG | Herbicidal n-(tetrazol-5-yl)-, n-(triazol-5-yl)- and n-(1,3,4-oxadiazol-2-yl)arylcarboxamide derivatives |
MX2018001523A (en) | 2015-08-06 | 2018-03-15 | Pioneer Hi Bred Int | Plant derived insecticidal proteins and methods for their use. |
CN114032251A (en) | 2015-08-07 | 2022-02-11 | 拜尔作物科学公司 | Root-preferred and stress-inducible promoters and uses thereof |
BR112018003545B1 (en) | 2015-08-25 | 2021-11-16 | Bayer Cropscience Aktiengesellschaft | REPLACED BENZOYLAMIDE KETOXYME |
EP3341483B1 (en) | 2015-08-28 | 2019-12-18 | Pioneer Hi-Bred International, Inc. | Ochrobactrum-mediated transformation of plants |
JP6978152B2 (en) | 2015-09-04 | 2021-12-08 | キージーン ナムローゼ フェンノートシャップ | Multiphase spore reproductive gene |
US10837024B2 (en) | 2015-09-17 | 2020-11-17 | Cellectis | Modifying messenger RNA stability in plant transformations |
JP6932125B2 (en) | 2015-09-28 | 2021-09-08 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | Acylated N- (1,2,5-oxadiazole-3-yl)-, N- (1,3,4-oxadiazole-2-yl)-, N- (tetrazole-5-yl)- And N- (triazole-5-yl) -arylcarboxamide, and their use as a herbicide |
US11732269B2 (en) | 2015-10-02 | 2023-08-22 | Monsanto Technology Llc | Recombinant maize B chromosome sequence and uses thereof |
AU2016331020B2 (en) | 2015-10-02 | 2021-04-29 | Keygene N.V. | Method for the production of haploid and subsequent doubled haploid plants |
BR112018006573A2 (en) | 2015-10-02 | 2018-10-09 | method for the production of haploid plants and subsequent duplicate haploids | |
US20180312864A1 (en) | 2015-10-22 | 2018-11-01 | Basf Se | Plants having increased tolerance to herbicides |
WO2017083092A1 (en) | 2015-11-10 | 2017-05-18 | Dow Agrosciences Llc | Methods and systems for predicting the risk of transgene silencing |
ES2939977T3 (en) | 2015-11-25 | 2023-04-28 | Gilead Apollo Llc | Triazole ACC inhibitors and uses thereof |
EP3380480B1 (en) | 2015-11-25 | 2022-12-14 | Gilead Apollo, LLC | Pyrazole acc inhibitors and uses thereof |
HUE053175T2 (en) | 2015-11-25 | 2021-06-28 | Gilead Apollo Llc | Ester acc inhibitors and uses thereof |
MA43177A (en) | 2015-12-15 | 2018-09-12 | Bayer Cropscience Lp | BRASSICAE RESISTANT TO PLASMODIOPHORA BRASSICAE (HERNIA) |
MX2018007393A (en) | 2015-12-16 | 2018-08-15 | Syngenta Participations Ag | Genetic regions & genes associated with increased yield in plants. |
CR20180370A (en) | 2015-12-17 | 2018-10-18 | Basf Se | BENZAMIDA COMPOUNDS AND THEIR USES AS HERBICIDES |
US9980448B2 (en) | 2015-12-18 | 2018-05-29 | Monsanto Technology Llc | Cotton variety 15R515B2XF |
US9854762B2 (en) | 2015-12-18 | 2018-01-02 | Monsanto Technology Llc | Cotton variety 15R509B2XF |
US9854761B2 (en) | 2015-12-18 | 2018-01-02 | Monsanto Technology Llc | Cotton variety 15R510B2XF |
US9961845B2 (en) | 2015-12-18 | 2018-05-08 | Monsanto Technology Llc | Cotton variety 15R551B2XF |
BR112018012887B1 (en) | 2015-12-22 | 2024-02-06 | Pioneer Hi-Bred International, Inc | EXPRESSION CASSETTE, VECTOR, METHODS FOR OBTAINING PLANT CELL AND TRANSGENIC PLANT, METHODS FOR EXPRESSING A POLYNUCLEOTIDE |
CN105399674B (en) | 2015-12-31 | 2017-02-15 | 青岛清原化合物有限公司 | Pyrazole compound or salt thereof, and preparation method, herbicide composition and application thereof |
CA3011521A1 (en) | 2016-01-26 | 2017-08-03 | Monsanto Technology Llc | Compositions and methods for controlling insect pests |
WO2017134601A1 (en) | 2016-02-02 | 2017-08-10 | Cellectis | Modifying soybean oil composition through targeted knockout of the fad3a/b/c genes |
WO2017136204A1 (en) | 2016-02-05 | 2017-08-10 | Pioneer Hi-Bred International, Inc. | Genetic loci associated with brown stem rot resistance in soybean and methods of use |
WO2017140612A1 (en) | 2016-02-18 | 2017-08-24 | Bayer Cropscience Aktiengesellschaft | Quinazolinedione-6-carbonyl derivatives and their use as herbicides |
UY37137A (en) | 2016-02-24 | 2017-09-29 | Merial Inc | ANTIPARASITARY COMPOUNDS OF ISOXAZOLINE, INJECTABLE FORMULATIONS OF PROLONGED ACTION THAT INCLUDE THEM, METHODS AND USES OF THE SAME |
WO2017144402A1 (en) | 2016-02-24 | 2017-08-31 | Bayer Cropscience Aktiengesellschaft | N-(5-halogen-1,3,4-oxadiazol-2-yl)aryl carboxylic acid amides and the use thereof as herbicides |
EA201891962A1 (en) | 2016-03-07 | 2019-04-30 | Байер Кропсайенс Акциенгезельшафт | HERBICIDE COMPOSITIONS CONTAINING ACTIVE CONNECTIONS FROM GRFD INHIBITORS GROUP, SAFENEROV AND TRIAZINS |
US20190098899A1 (en) | 2016-03-10 | 2019-04-04 | Basf Se | Fungicidal mixtures iii comprising strobilurin-type fungicides |
US10219463B2 (en) | 2016-03-25 | 2019-03-05 | Monsanto Technology Llc | Cotton variety 15R513B2XF |
WO2017178322A1 (en) | 2016-04-11 | 2017-10-19 | Bayer Cropscience Nv | Seed-specific and endosperm-preferential promoters and uses thereof |
US10975380B2 (en) | 2016-04-11 | 2021-04-13 | Basf Agricultural Solutions Seed, Us Llc | Seed-specific and endosperm-preferental promoters and uses thereof |
CA3020367A1 (en) | 2016-04-13 | 2017-10-19 | Bayer Cropscience Nv | Seed-and funiculus-preferential promoters and uses thereof |
WO2017178368A1 (en) | 2016-04-13 | 2017-10-19 | Bayer Cropscience Nv | Seed-specific and embryo-preferential promoters and uses thereof |
EP3960863A1 (en) | 2016-05-04 | 2022-03-02 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
EP3054014A3 (en) | 2016-05-10 | 2016-11-23 | BASF Plant Science Company GmbH | Use of a fungicide on transgenic plants |
JP2019516373A (en) | 2016-05-20 | 2019-06-20 | キージーン ナムローゼ フェンノートシャップ | Method of producing haploid and subsequent doubled haploid plants |
CN109154003A (en) | 2016-05-20 | 2019-01-04 | 巴斯夫农业公司 | Double transit peptides for target polypeptide |
EP3245872A1 (en) | 2016-05-20 | 2017-11-22 | BASF Agro B.V. | Pesticidal compositions |
WO2017207368A1 (en) | 2016-06-02 | 2017-12-07 | BASF Agro B.V. | Fungicidal compositions |
US20190185867A1 (en) | 2016-06-16 | 2019-06-20 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
EP4032965A1 (en) | 2016-06-16 | 2022-07-27 | Nuseed Nutritional Australia Pty Ltd | Elite event canola ns-b50027-4 |
EA201892783A1 (en) | 2016-06-16 | 2019-07-31 | НЬЮСИД ПиТиВай ЛТД. | INBREED TRANSGENIC CANOLA LINE NS-B50027-4 AND ITS SEEDS |
US10982226B2 (en) | 2016-06-20 | 2021-04-20 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Green alga bicarbonate transporter and uses thereof |
WO2017220467A1 (en) | 2016-06-24 | 2017-12-28 | Bayer Cropscience Aktiengesellschaft | 3-amino-1,2,4-triazine derivatives and their use for controlling undesired plant growth |
US20190194676A1 (en) | 2016-06-24 | 2019-06-27 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
CA3029271A1 (en) | 2016-06-28 | 2018-01-04 | Cellectis | Altering expression of gene products in plants through targeted insertion of nucleic acid sequences |
EP3475427A4 (en) | 2016-06-28 | 2019-11-06 | Monsanto Technology LLC | Methods and compositions for use in genome modification in plants |
US10136599B2 (en) | 2016-06-29 | 2018-11-27 | Monsanto Technology Llc | Cotton variety 15R535B2XF |
EP3954202A1 (en) | 2016-07-01 | 2022-02-16 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins from plants and methods for their use |
US20210292778A1 (en) | 2016-07-12 | 2021-09-23 | Pioneer Hi-Bred International, Inc. | Compositions and methods to control insect pests |
CA3030803A1 (en) | 2016-07-15 | 2018-01-18 | Basf Se | Plants having increased tolerance to herbicides |
BR112019001483A2 (en) | 2016-07-27 | 2019-09-10 | Basf Agro Bv | method for controlling unwanted vegetation at a plantation site |
RU2019104918A (en) | 2016-07-29 | 2020-08-28 | Байер Кропсайенс Акциенгезельшафт | COMBINATIONS OF ACTIVE COMPOUNDS AND METHODS FOR PROTECTING PLANT REPRODUCTION MATERIAL |
US10426110B2 (en) | 2016-09-07 | 2019-10-01 | Agrigenetics, Inc. | Canola inbred restorer line CL2503899R |
US10588281B2 (en) | 2016-09-07 | 2020-03-17 | Agrigenetics, Inc. | Canola hybrid cultivar G5428584H |
US10244716B2 (en) | 2016-09-07 | 2019-04-02 | Agrigenetics, Inc. | Canola hybrid cultivar CL3701975H |
US10463004B2 (en) | 2016-09-07 | 2019-11-05 | Agrigenetics, Inc. | Canola inbred line G1466454A/B |
US10420296B2 (en) | 2016-09-07 | 2019-09-24 | Agrigenetics, Inc. | Canola inbred restorer line G263532R |
BR112019005668A2 (en) | 2016-09-22 | 2019-06-04 | Bayer Ag | new triazole derivatives |
WO2018054829A1 (en) | 2016-09-22 | 2018-03-29 | Bayer Cropscience Aktiengesellschaft | Novel triazole derivatives and their use as fungicides |
US20190225974A1 (en) | 2016-09-23 | 2019-07-25 | BASF Agricultural Solutions Seed US LLC | Targeted genome optimization in plants |
US11266174B2 (en) | 2016-10-07 | 2022-03-08 | Altria Client Services Llc | Tobacco plants having increased nitrogen efficiency and methods of using such plants |
US20190261630A1 (en) | 2016-10-26 | 2019-08-29 | Bayer Cropscience Aktiengesellschaft | Use of pyraziflumid for controlling sclerotinia spp in seed treatment applications |
EP4050021A1 (en) | 2016-11-01 | 2022-08-31 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
CA3042857A1 (en) | 2016-11-16 | 2018-05-24 | Cellectis | Methods for altering amino acid content in plants through frameshift mutations |
CN110035660B (en) | 2016-12-07 | 2021-10-26 | 拜耳作物科学股份公司 | Herbicidal combination containing triafamone and indoxachlor |
US20190387661A1 (en) | 2016-12-08 | 2019-12-26 | Bayer Cropscience Aktiengesellschaft | Use of insecticides for controlling wireworms |
AU2017370528B2 (en) | 2016-12-08 | 2024-01-11 | Syngenta Participations Ag | Methods for improving transformation frequency |
EP3332645A1 (en) | 2016-12-12 | 2018-06-13 | Bayer Cropscience AG | Use of substituted pyrimidine diones or their salts as agents to combat abiotic plant stress |
WO2018108627A1 (en) | 2016-12-12 | 2018-06-21 | Bayer Cropscience Aktiengesellschaft | Use of substituted indolinylmethyl sulfonamides, or the salts thereof for increasing the stress tolerance of plants |
CA3044728A1 (en) | 2016-12-20 | 2018-06-28 | BASF Agro B.V. | Plants having increased tolerance to herbicides |
EP3338552A1 (en) | 2016-12-21 | 2018-06-27 | Basf Se | Use of a tetrazolinone fungicide on transgenic plants |
US9963714B1 (en) | 2016-12-30 | 2018-05-08 | Monsanto Technology Llc | Cotton variety 16R247NRB2XF |
EP3354738A1 (en) | 2017-01-30 | 2018-08-01 | Kws Saat Se | Transgenic maize plant exhibiting increased yield and drought tolerance |
WO2018141575A1 (en) | 2017-02-01 | 2018-08-09 | Basf Se | Emulsifiable concentrate |
RU2758117C2 (en) | 2017-02-07 | 2021-10-26 | Юниверсити Оф Кентукки Рисёрч Фаундейшн | Method for increasing the content of sucrose ester in tobacco plants |
KR20190116987A (en) | 2017-02-13 | 2019-10-15 | 바이엘 크롭사이언스 악티엔게젤샤프트 | Substituted benzyl-4-aminopicolinic acid esters and pyrimidino-4-carboxylic acid esters, methods for their preparation, and their use as herbicides and plant growth regulators |
EP3360872A1 (en) | 2017-02-13 | 2018-08-15 | Bayer CropScience Aktiengesellschaft | Substituted benzyl-4-aminopicolinic acid esters and pyrimidin-4-carboxylic acid ester, process for their preparation and use as herbicides and regulators of plant growth |
US10499605B2 (en) | 2017-03-03 | 2019-12-10 | Monsanto Technology Llc | Cotton variety 16R251NRB2XF |
US11542519B2 (en) | 2017-03-23 | 2023-01-03 | Basf Se | Anther-specific promoter and uses thereof |
EP3378316A1 (en) | 2017-03-24 | 2018-09-26 | Bayer Aktiengesellschaft | Herbicidal mixtures |
EP3378315A1 (en) | 2017-03-24 | 2018-09-26 | Bayer CropScience Aktiengesellschaft | Herbicidal mixtures comprising 2-[2,4-dichlorophenyl)methyl]-4,4-dimethyl-3-isoxazolidinone |
WO2018177871A1 (en) | 2017-03-30 | 2018-10-04 | Bayer Cropscience Aktiengesellschaft | Substituted n-(-1,3,4-oxadiazole-2-yl)aryl carboxamides and the use thereof as herbicides |
JP7107962B2 (en) | 2017-04-05 | 2022-07-27 | バイエル・クロップサイエンス・アクチェンゲゼルシャフト | 2-Amino-5-oxyalkyl-pyrimidine derivatives and their use for controlling unwanted plant growth |
CA3060622A1 (en) | 2017-04-25 | 2018-11-01 | Cellectis | Alfalfa with reduced lignin composition |
CA3062225A1 (en) | 2017-05-04 | 2018-11-08 | Bayer Cropscience Aktiengesellschaft | 4-difluoromethyl benzoyl amides with herbicidal action |
EP3618620A1 (en) | 2017-05-04 | 2020-03-11 | Bayer CropScience Aktiengesellschaft | Herbicide safener compositions containing quinazolinedione-6-carbonyl derivatives |
US11591601B2 (en) | 2017-05-05 | 2023-02-28 | The Broad Institute, Inc. | Methods for identification and modification of lncRNA associated with target genotypes and phenotypes |
EP3630734A1 (en) | 2017-05-30 | 2020-04-08 | Basf Se | Benzamide compounds and their use as herbicides |
AU2018276360A1 (en) | 2017-05-30 | 2019-12-19 | Basf Se | Benzamide compounds and their use as herbicides II |
CA2968905A1 (en) | 2017-05-31 | 2018-11-30 | Bayer Cropscience Lp | Canola hybrid variety 5cn0125 |
CA2968938A1 (en) | 2017-05-31 | 2018-11-30 | Bayer Cropscience Lp | Canola hybrid variety 5cn0130 |
US11613522B2 (en) | 2017-06-13 | 2023-03-28 | Bayer Aktiengesellschaft | Herbicidally active 3-phenylisoxazoline-5-carboxamides of tetrahydro- and dihydrofurancarboxamides |
EP3638665B1 (en) | 2017-06-13 | 2021-07-21 | Bayer Aktiengesellschaft | Herbicidal 3-phenylisoxazoline-5-carboxamides of tetrahydro and dihydrofuran carboxylic acids and esters |
AR112112A1 (en) | 2017-06-20 | 2019-09-18 | Basf Se | BENZAMIDE COMPOUNDS AND THEIR USE AS HERBICIDES |
AU2018287131B2 (en) | 2017-06-23 | 2023-10-12 | Basf Se | Pesticidal mixtures comprising a pyrazole compound |
WO2018237107A1 (en) | 2017-06-23 | 2018-12-27 | University Of Kentucky Research Foundation | Method |
WO2019001793A1 (en) | 2017-06-26 | 2019-01-03 | Bayer Cropscience Nv | Regeneration of cereals |
CN110891923A (en) | 2017-07-10 | 2020-03-17 | 巴斯夫欧洲公司 | Mixtures comprising Urease Inhibitors (UI) and nitrification inhibitors, such as 2- (3, 4-dimethyl-1H-pyrazol-1-yl) succinic acid (DMPSA) or 3, 4-dimethylpyrazolium glycolate (DMPG) |
WO2019016385A1 (en) | 2017-07-21 | 2019-01-24 | Basf Se | Benzamide compounds and their use as herbicides |
BR112020001585A2 (en) | 2017-07-27 | 2020-08-11 | Basf Se | uses of a composition and method to control harmful plants in a glufosinate-tolerant field culture |
WO2019025153A1 (en) | 2017-07-31 | 2019-02-07 | Bayer Cropscience Aktiengesellschaft | Use of substituted n-sulfonyl-n'-aryl diaminoalkanes and n-sulfonyl-n'-heteroaryl diaminoalkanes or salts thereof for increasing the stress tolerance in plants |
TWI771440B (en) | 2017-08-04 | 2022-07-21 | 德商拜耳廠股份有限公司 | 3-acylbenzamides and their use as herbicides |
CN111164077B (en) | 2017-08-17 | 2023-12-19 | 拜耳公司 | Herbicidal 3-phenyl-5-trifluoromethyl isoxazoline-5-carboxamides of cyclopentylcarboxylic acids and esters thereof |
EP3673054A4 (en) | 2017-08-22 | 2021-06-02 | Napigen, Inc. | Organelle genome modification using polynucleotide guided endonuclease |
EP3675625A1 (en) | 2017-09-01 | 2020-07-08 | Altria Client Services LLC | Methods and compositions related to improved nitrogen utilization efficiency in tobacco |
CA3073848A1 (en) | 2017-09-21 | 2019-03-28 | The Broad Institute, Inc. | Systems, methods, and compositions for targeted nucleic acid editing |
CN111373046A (en) | 2017-09-25 | 2020-07-03 | 先锋国际良种公司 | Tissue-preferred promoters and methods of use |
EP3360417A1 (en) | 2017-11-02 | 2018-08-15 | Bayer CropScience Aktiengesellschaft | Use of sulfonylindol as herbicide |
CN111246738A (en) | 2017-11-15 | 2020-06-05 | 巴斯夫欧洲公司 | Tank mix |
JP7220713B2 (en) | 2017-11-20 | 2023-02-10 | バイエル アクチェンゲゼルシャフト | herbicidally active bicyclic benzamide |
AU2018376915A1 (en) | 2017-11-29 | 2020-05-21 | Basf Se | Plants having increased tolerance to herbicides |
WO2019105995A1 (en) | 2017-11-29 | 2019-06-06 | Basf Se | Benzamide compounds and their use as herbicides |
EP3707640A1 (en) | 2017-12-03 | 2020-09-16 | Seedx Technologies Inc. | Systems and methods for sorting of seeds |
WO2019106638A1 (en) | 2017-12-03 | 2019-06-06 | Seedx Technologies Inc. | Systems and methods for sorting of seeds |
CN111448194A (en) | 2017-12-04 | 2020-07-24 | 拜耳作物科学股份公司 | 3-amino- [1,2, 4] -triazole derivatives and their use for controlling undesired vegetation |
WO2019123194A1 (en) | 2017-12-20 | 2019-06-27 | Pi Industries Ltd. | Anthranilamides, their use as insecticide and processes for preparing the same. |
BR112020012455B1 (en) | 2017-12-20 | 2024-04-30 | Pi Industries Ltd | PYRAZOLOPYRIDINE-DIAMIDES, THEIR USE AS INSECTICIDE AND PROCESSES FOR THEIR PREPARATION |
WO2019122347A1 (en) | 2017-12-22 | 2019-06-27 | Basf Se | N-(1,2,5-oxadiazol-3-yl)-benzamide compounds and their use as herbicides |
WO2019122345A1 (en) | 2017-12-22 | 2019-06-27 | Basf Se | Benzamide compounds and their use as herbicides |
US10856513B2 (en) | 2017-12-28 | 2020-12-08 | Monsanto Technology Llc | Cotton variety 16R338B3XF |
US10863715B2 (en) | 2017-12-28 | 2020-12-15 | Monsanto Technology Llc | Cotton variety 16R343B3XF |
US10939656B2 (en) | 2017-12-28 | 2021-03-09 | Monsanto Technology Llc | Cotton variety 16R228NRB2XF |
US10869455B2 (en) | 2017-12-28 | 2020-12-22 | Monsanto Technology Llc | Cotton variety 16R345B3XF |
US10863714B2 (en) | 2017-12-28 | 2020-12-15 | Monsanto Technology Llc | Cotton variety 16R330B3XF |
US10842122B2 (en) | 2017-12-28 | 2020-11-24 | Monsanto Technology Llc | Cotton variety 16R123XF |
US10842121B2 (en) | 2017-12-28 | 2020-11-24 | Monsanto Technology Llc | Cotton variety 16R141XF |
US10925248B2 (en) | 2017-12-28 | 2021-02-23 | Monsanto Technology Llc | Cotton variety 16R225NRB2XF |
US10856512B2 (en) | 2017-12-28 | 2020-12-08 | Monsanto Technology Llc | Cotton variety 16R336B3XF |
US10842120B2 (en) | 2017-12-28 | 2020-11-24 | Monsanto Technology Llc | Cotton variety 16R125XF |
US10874079B2 (en) | 2017-12-28 | 2020-12-29 | Monsanto Technology Llc | Cotton variety 16R351B3XF |
US10842123B2 (en) | 2017-12-28 | 2020-11-24 | Monsanto Technology Llc | Cotton variety 16R324B3XF |
US10863713B2 (en) | 2017-12-28 | 2020-12-15 | Monsanto Technology Llc | Cotton variety 16R341B3XF |
US10849302B2 (en) | 2017-12-28 | 2020-12-01 | Monsanto Technology Llc | Cotton variety 16R335B3XF |
EP3508480A1 (en) | 2018-01-08 | 2019-07-10 | Basf Se | Benzamide compounds and their use as herbicides |
BR112020013929A2 (en) | 2018-01-17 | 2020-12-01 | Basf Se | plants or parts of the plant, seed, plant cells, plant product, progeny or descending plant, methods for controlling weeds, for producing a plant and for producing a descending plant, nucleic acid molecule, expression cassette, vector, polypeptide, method to produce a plant product and plant product |
JP7217751B2 (en) | 2018-01-25 | 2023-02-03 | バイエル・アクチエンゲゼルシヤフト | 3-phenylisoxazoline-5-carboxamides of cyclopentenylcarboxylic acid derivatives exhibiting herbicidal activity |
BR112020014473A2 (en) | 2018-01-30 | 2020-12-01 | Pi Industries Ltd. | new anthranilamides, their use as an insecticide and processes to prepare them |
WO2019149260A1 (en) | 2018-02-02 | 2019-08-08 | 青岛清原化合物有限公司 | Pyridazinol compound, derivative thereof, preparation method therefor, herbicidal composition and use thereof |
WO2019162309A1 (en) | 2018-02-21 | 2019-08-29 | Basf Se | Benzamide compounds and their use as herbicides |
WO2019162308A1 (en) | 2018-02-21 | 2019-08-29 | Basf Se | Benzamide compounds and their use as herbicides |
US10968257B2 (en) | 2018-04-03 | 2021-04-06 | The Broad Institute, Inc. | Target recognition motifs and uses thereof |
CA3004115A1 (en) | 2018-05-07 | 2019-11-07 | Bayer Cropscience Lp | Canola hybrid variety 6cn0122 |
CN112334446A (en) | 2018-05-15 | 2021-02-05 | 拜耳公司 | 2-bromo-6-alkoxyphenyl-substituted pyrrolin-2-ones and their use as herbicides |
AR115089A1 (en) | 2018-05-15 | 2020-11-25 | Bayer Ag | 2-ALKYL-6-ALCOXIFENIL-3-PIRROLIN-2-ONAS SPECIALLY SUBSTITUTED AND THEIR USE AS HERBICIDES |
WO2019219584A1 (en) | 2018-05-15 | 2019-11-21 | Bayer Aktiengesellschaft | New spiro cyclohexyl pyrrolin-2-ones and their use as herbicides |
AR115087A1 (en) | 2018-05-15 | 2020-11-25 | Bayer Ag | 3- (4-ALKINYL-6-ALCOXI-2-CHLOROPHENIL) -3-PYRROLIN-2-ONAS, A METHOD FOR ITS PREPARATION AND ITS USE AS HERBICIDES |
EP3569065A1 (en) | 2018-05-17 | 2019-11-20 | Biotensidon International AG | Composition with herbicidal activity |
CA3096516A1 (en) | 2018-05-22 | 2019-11-28 | Pioneer Hi-Bred International, Inc. | Plant regulatory elements and methods of use thereof |
WO2019228787A1 (en) | 2018-05-29 | 2019-12-05 | Bayer Aktiengesellschaft | Specifically substituted 2-alkyl-6-alkoxyphenyl-3-pyrrolin-2-ones and their use as herbicides |
WO2019228788A1 (en) | 2018-05-29 | 2019-12-05 | Bayer Aktiengesellschaft | 2-bromo-6-alkoxyphenyl-substituted pyrrolin-2-ones and their use as herbicides |
US20210323950A1 (en) | 2018-06-04 | 2021-10-21 | Bayer Aktiengesellschaft | Herbicidally active bicyclic benzoylpyrazoles |
AU2019296278A1 (en) | 2018-06-27 | 2021-02-04 | Basf Se | Thermostable rubisco activase and uses thereof |
CA3097915A1 (en) | 2018-06-28 | 2020-01-02 | Pioneer Hi-Bred International, Inc. | Methods for selecting transformed plants |
JP2021529820A (en) | 2018-07-16 | 2021-11-04 | バイエル・アクチエンゲゼルシヤフト | Herbicide mixture containing acronifen and symmethyrin |
EP3826466A1 (en) | 2018-07-26 | 2021-06-02 | Bayer Aktiengesellschaft | Use of the succinate dehydrogenase inhibitor fluopyram for controlling root rot complex and/or seedling disease complex caused by rhizoctonia solani, fusarium species and pythium species in brassicaceae species |
WO2020035486A1 (en) | 2018-08-13 | 2020-02-20 | Aarhus Universitet | Genetically altered plants expressing heterologous receptors that recognize lipo-chitooligosaccharides |
WO2020035488A1 (en) | 2018-08-13 | 2020-02-20 | Aarhus Universitet | Genetically altered lysm receptors with altered agonist specificity and affinity |
BR112021004865A2 (en) | 2018-09-17 | 2021-06-01 | Bayer Aktiengesellschaft | use of the fungicide isoflucypram to control claviceps purpurea and reduce sclerotia in cereals |
US20220039383A1 (en) | 2018-09-17 | 2022-02-10 | Bayer Aktiengesellschaft | Use of the Succinate Dehydrogenase Inhibitor Fluopyram for Controlling Claviceps Purpurea and Reducing Sclerotia in Cereals |
WO2020058010A1 (en) | 2018-09-19 | 2020-03-26 | Basf Se | Pesticidal mixtures comprising a mesoionic compound |
WO2020058062A1 (en) | 2018-09-19 | 2020-03-26 | Bayer Aktiengesellschaft | Herbicidally active substituted phenylpyrimidine hydrazides |
CA3112042A1 (en) | 2018-09-28 | 2020-04-02 | Basf Se | Method of controlling pests by seed treatment application of a mesoionic compound or mixture thereof |
BR112021008329A2 (en) | 2018-10-31 | 2021-08-03 | Pioneer Hi-Bred International, Inc. | compositions and methods for ochrobactrum-mediated plant transformation |
CN111253333A (en) | 2018-11-30 | 2020-06-09 | 青岛清原化合物有限公司 | N- (1,3, 4-oxadiazole-2-yl) aryl formamide or salt thereof, preparation method, herbicidal composition and application |
AU2019392735A1 (en) | 2018-12-06 | 2021-06-17 | Wageningen Universiteit | Methods of genetically altering a plant NIN-gene to be responsive to cytokinin |
CA3124110A1 (en) | 2018-12-17 | 2020-06-25 | The Broad Institute, Inc. | Crispr-associated transposase systems and methods of use thereof |
US10918070B2 (en) | 2018-12-19 | 2021-02-16 | Monsanto Technology Llc | Cotton variety 17R806B3XF |
US10905086B2 (en) | 2018-12-19 | 2021-02-02 | Monsanto Technology Llc | Cotton variety 17R820B3XF |
US10918071B2 (en) | 2018-12-19 | 2021-02-16 | Monsanto Technology Llc | Cotton variety 16R346B3XF |
US10905085B2 (en) | 2018-12-19 | 2021-02-02 | Monsanto Technology Llc | Cotton variety 17R808B3XF |
US10874081B2 (en) | 2018-12-19 | 2020-12-29 | Monsanto Technology Llc | Cotton variety 17R709XF |
US20220098153A1 (en) | 2018-12-27 | 2022-03-31 | Qingdao Kingagroot Chemical Compound Co., Ltd. | R-type pyridyloxycarboxylic acid, salt and ester derivative thereof, and preparation method therefor, and herbicidal composition and application thereof |
US10918074B2 (en) | 2019-01-09 | 2021-02-16 | Monsanto Technology Llc | Cotton variety 17R844B3XF |
US10939658B2 (en) | 2019-01-09 | 2021-03-09 | Monsanto Technology Llc | Cotton variety 16R232B2XF |
US10939659B2 (en) | 2019-01-09 | 2021-03-09 | Monsanto Technology Llc | Cotton variety 16R353B3XF |
US10918076B2 (en) | 2019-01-09 | 2021-02-16 | Monsanto Technology Llc | Cotton variety 17R817B3XF |
US10939661B2 (en) | 2019-01-09 | 2021-03-09 | Monsanto Technology Llc | Cotton variety 17R815B3XF |
US10939660B2 (en) | 2019-01-09 | 2021-03-09 | Monsanto Technology Llc | Cotton variety 17R740XF |
US10925250B2 (en) | 2019-01-09 | 2021-02-23 | Monsanto Technology Llc | Cotton variety 17R816B3XF |
US10918073B2 (en) | 2019-01-09 | 2021-02-16 | Monsanto Technology Llc | Cotton variety 17R819B3XF |
US10918034B2 (en) | 2019-01-09 | 2021-02-16 | Monsanto Technology Llc | Cotton variety 17R737XF |
US10918075B2 (en) | 2019-01-09 | 2021-02-16 | Monsanto Technology Llc | Cotton variety 17R845B3XF |
US10918072B2 (en) | 2019-01-09 | 2021-02-16 | Monsanto Technology Llc | Cotton variety 17R814B3XF |
US10918077B2 (en) | 2019-01-09 | 2021-02-16 | Monsanto Technology Llc | Cotton variety 17R827B3XF |
US10905087B2 (en) | 2019-01-09 | 2021-02-02 | Monsanto Technology Llc | Cotton variety 16R246NRB2XF |
US10925249B2 (en) | 2019-01-09 | 2021-02-23 | Monsanto Technology Llc | Cotton variety 17R933NRB3XF |
EP3680223A1 (en) | 2019-01-10 | 2020-07-15 | Basf Se | Mixture comprising an urease inhibitor (ui) and a nitrification inhibitor (ni) such as an ni mixture comprising 2-(3,4-dimethyl-1h-pyrazol-1-yl)succinic acid (dmpsa) and dicyandiamide (dcd) |
EP3892618B1 (en) | 2019-01-14 | 2024-04-10 | Qingdao KingAgroot Chemical Compound Co., Ltd. | 4-pyridinyl formamide compound or derivative thereof, preparation method therefor, herbicidal composition and use thereof |
EA202191910A1 (en) | 2019-01-14 | 2021-11-16 | Байер Акциенгезельшафт | HERBICIDAL SUBSTITUTED N-TETRAZOLYL-ARYL CARBOXAMIDES |
WO2020169509A1 (en) | 2019-02-20 | 2020-08-27 | Bayer Aktiengesellschaft | Herbicidally active 4-(4-trifluormethyl-6-cycloropylpyrazolyl)pyrimidines |
AR118243A1 (en) | 2019-03-07 | 2021-09-22 | Pi Industries Ltd | FUSED HETEROCYCLIC COMPOUNDS AND THEIR USE AS PEST CONTROL AGENTS |
BR112021017924A2 (en) | 2019-03-12 | 2021-11-16 | Bayer Ag | Herbicidally active 3-phenylisoxazoline-5-carboxamides of cyclopentenyl-carboxylic acid esters containing s |
JP2022525174A (en) | 2019-03-15 | 2022-05-11 | バイエル・アクチエンゲゼルシヤフト | Specifically substituted 3- (2-alkoxy-6-alkyl-4-propynylphenyl) -3-pyrroline-2-ones and their use as herbicides |
EP3938348A1 (en) | 2019-03-15 | 2022-01-19 | Bayer Aktiengesellschaft | Novel 3-(2-brom-4-alkynyl-6-alkoxyphenyl)-3-pyrrolin-2-ones and their use as herbicides |
US20230059463A1 (en) | 2019-03-15 | 2023-02-23 | Bayer Aktiengesellschaft | 3-(2-bromo-4-alkynyl-6-alkoxyphenyl)-substituted 5-spirocyclohexyl-3-pyrrolin-2-ones and their use as herbicides |
EP3938346A1 (en) | 2019-03-15 | 2022-01-19 | Bayer Aktiengesellschaft | Specifically substituted 3-(2-halogen-6-alkyl-4-propinylphenyl)-3-pyrrolin-2-ones and to the use thereof as herbicides |
JP2022524861A (en) | 2019-03-15 | 2022-05-10 | バイエル・アクチエンゲゼルシヤフト | Specifically substituted 3-phenyl-5-spirocyclopentyl-3-pyrrolin-2-ones and their use as herbicides |
WO2020187995A1 (en) | 2019-03-21 | 2020-09-24 | University Of Essex Enterprises Limited | Methods of enhancing biomass in a plant through stimulation of rubp regeneration and electron transport |
CA3040289A1 (en) | 2019-04-12 | 2020-10-12 | BASF Agricultural Solutions Seed US LLC | Canola hybrid variety 7cn0298 |
CN113874388A (en) | 2019-05-29 | 2021-12-31 | 主基因有限公司 | Parthenogenesis genes |
US20220322661A1 (en) | 2019-06-03 | 2022-10-13 | Bayer Aktiengesellschaft | Adjuvant combinations as foliar uptake accelerator for herbicidal compositions |
EP3975720A1 (en) | 2019-06-03 | 2022-04-06 | Bayer Aktiengesellschaft | 1-phenyl-5-azinyl pyrazolyl-3-oxyalkyl acids and their use for controlling undesired plant growth |
AR119140A1 (en) | 2019-06-13 | 2021-11-24 | Pi Industries Ltd | FUSED HETEROCYCLIC COMPOUNDS AND THEIR USE AS PEST CONTROL AGENTS |
CA3047768A1 (en) | 2019-06-21 | 2020-12-21 | BASF Agricultural Solutions Seed US LLC | Canola hybrid variety 7cn0425 |
WO2021004838A2 (en) | 2019-07-05 | 2021-01-14 | BASF Agricultural Solutions Seed US LLC | Rubisco activase with reduced adp inhibition and uses thereof |
AU2020326547A1 (en) | 2019-08-02 | 2022-02-24 | Howard Hughes Medical Institute | Rubisco-binding protein motifs and uses thereof |
GB201911068D0 (en) | 2019-08-02 | 2019-09-18 | Univ Edinburgh | Modified higher plants |
US10993407B2 (en) | 2019-08-15 | 2021-05-04 | Monsanto Technology Llc | Cotton variety 17R818B3XF |
AR119794A1 (en) | 2019-08-19 | 2022-01-12 | Univ Aarhus | EXOPOLYSACCHARIDE RECEPTORS MODIFIED TO RECOGNIZE AND STRUCTURE THE MICROBIOTA |
WO2021033141A1 (en) | 2019-08-20 | 2021-02-25 | Pi Industries Ltd. | Fused heterocyclic compounds and their use as pest control agents |
AR119790A1 (en) | 2019-08-29 | 2022-01-12 | Pi Industries Ltd | ISOXAZOLINE COMPOUNDS AND THEIR USE AS PEST CONTROL AGENTS |
CN113574173A (en) | 2019-09-17 | 2021-10-29 | 北京大北农生物技术有限公司 | Mutant hydroxyphenylpyruvate dioxygenase polypeptide, coding gene and application thereof |
CN116803993A (en) | 2019-10-23 | 2023-09-26 | 青岛清原化合物有限公司 | Aryl formamide compound containing chiral sulfur oxide or salt thereof, preparation method, weeding composition and application |
MX2022005295A (en) | 2019-11-01 | 2022-05-24 | Purecircle Usa Inc | Stevia cultivar '18136109'. |
CN117567451A (en) | 2019-11-07 | 2024-02-20 | 青岛清原化合物有限公司 | Aromatic compound containing substituted isoxazoline, preparation method thereof, weeding composition and application |
US11154029B2 (en) | 2019-12-16 | 2021-10-26 | Monsanto Technology Llc | Cotton variety 18R445B3XF |
US11026392B1 (en) | 2019-12-16 | 2021-06-08 | Monsanto Technology Llc | Cotton variety 18R421B3XF |
US11051483B1 (en) | 2019-12-16 | 2021-07-06 | Monsanto Technology Llc | Cotton variety 18R448B3XF |
US11064672B2 (en) | 2019-12-16 | 2021-07-20 | Monsanto Technology Llc | Cotton variety 18R438B3XF |
US11039594B1 (en) | 2019-12-16 | 2021-06-22 | Monsanto Technology Llc | Cotton variety 18R423B3XF |
AU2020409657A1 (en) | 2019-12-19 | 2022-07-07 | Bayer Aktiengesellschaft | 1,5-diphenylpyrazolyl-3-oxyalkyl acids and 1-phenyl-5-thienylpyrazolyl-3-oxyalkyl acids and the use thereof for control of undesired plant growth |
CA3162521A1 (en) | 2019-12-23 | 2021-07-01 | Basf Se | Enzyme enhanced root uptake of agrochemical active compound |
BR112022013644A2 (en) | 2020-01-11 | 2022-09-13 | Qingdao Kingagroot Chemical Compound Co Ltd | IMINOARYL COMPOUND SUBSTITUTED BY CARBOXYLIC ACID DERIVATIVE, PREPARATION METHOD, HERBICIDAL COMPOSITION AND USE THEREOF |
CA3135347A1 (en) | 2020-01-16 | 2021-07-22 | Qingdao Kingagroot Chemical Compound Co., Ltd. | A fused-ring substituted aromatic compound, preparation method, herbicidal composition and use thereof |
US20230063109A1 (en) | 2020-01-16 | 2023-03-02 | Basf Se | Mixtures comprising nitrification inhibitors and carriers |
CA3164114A1 (en) | 2020-01-16 | 2021-07-22 | Gregor Pasda | Mixtures comprising a solid carrier comprising an urease inhibitor and a further solid carrier comprising a nitrification inhibitor |
US11134645B2 (en) | 2020-01-24 | 2021-10-05 | Monsanto Technology Llc | Cotton variety 18R441B3XF |
US11197454B2 (en) | 2020-01-24 | 2021-12-14 | Monsanto Technology Llc | Cotton variety 18R062 |
US11134646B2 (en) | 2020-01-24 | 2021-10-05 | Monsanto Technology Llc | Cotton variety 18R459B3XF |
US11206800B2 (en) | 2020-01-24 | 2021-12-28 | Monsanto Technology Llc | Cotton variety 18R067 |
US11310991B2 (en) | 2020-01-24 | 2022-04-26 | Monsanto Technology Llc | Cotton variety 18R540B3XF |
US11185046B2 (en) | 2020-01-24 | 2021-11-30 | Monsanto Technology Llc | Cotton variety 18R409B3XF |
US11129353B2 (en) | 2020-01-24 | 2021-09-28 | Monsanto Technology Llc | Cotton variety 18R419B3XF |
US11129352B2 (en) | 2020-01-24 | 2021-09-28 | Monsanto Technology Llc | Cotton variety 18R418B3XF |
US11129354B2 (en) | 2020-01-24 | 2021-09-28 | Monsanto Technology Llc | Cotton variety 18R435B3XF |
US11197455B2 (en) | 2020-02-12 | 2021-12-14 | Monsanto Technology Llc | Cotton variety 18R410B3XF |
US11330787B2 (en) | 2020-02-12 | 2022-05-17 | Monsanto Technology Llc | Cotton variety 18R420B3XF |
US11154030B2 (en) | 2020-02-12 | 2021-10-26 | Monsanto Technology Llc | Cotton variety 16R020 |
AR121344A1 (en) | 2020-02-18 | 2022-05-11 | Pi Industries Ltd | FUSED HETEROCYCLIC COMPOUNDS AND THEIR USE AS PEST CONTROL AGENTS |
WO2021170794A1 (en) | 2020-02-28 | 2021-09-02 | Cambridge Enterprise Limited | Methods, plants and compositions for overcoming nutrient suppression of mycorrhizal symbiosis |
WO2021204669A1 (en) | 2020-04-07 | 2021-10-14 | Bayer Aktiengesellschaft | Substituted isophthalic acid diamides |
EP4132917B1 (en) | 2020-04-07 | 2024-01-24 | Bayer Aktiengesellschaft | Substituted isophtalic acid diamides |
CA3179386A1 (en) | 2020-04-07 | 2021-10-14 | Bayer Aktiengesellschaft | Substituted isophthalic acid diamides |
CN115667231A (en) | 2020-04-07 | 2023-01-31 | 拜耳公司 | Substituted isophthalic acid diamides and their use as herbicides |
WO2021205000A2 (en) | 2020-04-09 | 2021-10-14 | R.J. Reynolds Tobacco Company | Method |
WO2021204884A1 (en) | 2020-04-09 | 2021-10-14 | Bayer Aktiengesellschaft | 3-(4-alkenyl-phenyl)-3-pyrrolin-2-ones and their use as herbicides |
WO2021209486A1 (en) | 2020-04-15 | 2021-10-21 | Bayer Aktiengesellschaft | Specifically substituted pyrroline-2-ones and their use as herbicides |
US11672218B2 (en) | 2020-04-24 | 2023-06-13 | BASF Agricultural Solutions Seed US LLC | Canola hybrid variety 7CN0065 |
US11997965B2 (en) | 2020-04-24 | 2024-06-04 | BASF Agricultural Solutions Seed US LLC | Canola hybrid variety 8CN0001 |
US11997966B2 (en) | 2020-04-24 | 2024-06-04 | BASF Agricultural Solutions Seed US LLC | Canola hybrid variety 7CN0020 |
EP4143181A1 (en) | 2020-04-29 | 2023-03-08 | Bayer Aktiengesellschaft | 1-pyrazinylpyrazolyl-3-oxyalkyl acids and their derivatives, and their use for control of undesired plant growth |
CN116323952A (en) | 2020-05-19 | 2023-06-23 | 奥尔胡斯大学 | LYSM receptor motif |
BR112022022128A2 (en) | 2020-05-27 | 2022-12-13 | Bayer Ag | SPECIFICALLY SUBSTITUTED PIRROLIN-2-ONES AND THEIR USE AS HERBICIDES |
TW202216700A (en) | 2020-07-02 | 2022-05-01 | 印度商皮埃企業有限公司 | Isoxazoline compounds and their use as pest control agents |
CN116234444A (en) | 2020-07-06 | 2023-06-06 | 皮埃企业有限公司 | Mixtures with insecticidal activity comprising sulfur-containing heterocyclylalkoxy compounds, oxides or salts thereof |
US20230235352A1 (en) | 2020-07-14 | 2023-07-27 | Pioneer Hi-Bred International, Inc. | Insecticidal proteins and methods for their use |
TW202220557A (en) | 2020-07-27 | 2022-06-01 | 印度商皮埃企業有限公司 | A pesticidally active mixture comprising pyrazolopyridine anthranilamide compound, oxides or salts thereof |
EP4203677A1 (en) | 2020-08-26 | 2023-07-05 | Vindara, Inc. | Method and/or compositions for lettuce (lactuca sativa) breeding and/or varieties developed thereby |
IL301700A (en) | 2020-10-13 | 2023-05-01 | Keygene Nv | Modified promoter of a parthenogenesis gene |
JP2023549462A (en) | 2020-10-23 | 2023-11-27 | バイエル・アクチエンゲゼルシヤフト | 1-(Pyridyl)-5-azinylpyrazole derivatives and their use for the control of undesirable plant growth |
CN116917283A (en) | 2020-12-11 | 2023-10-20 | 皮埃企业有限公司 | Isoxazoline compounds and their use as pest control agents |
AU2021410073A1 (en) | 2020-12-21 | 2023-07-06 | BASF Agricultural Solutions Seed US LLC | Brassica napus plants comprising an improved fertility restorer |
US11432519B2 (en) | 2020-12-22 | 2022-09-06 | Monsanto Technology Llc | Cotton variety 19R227B3XF |
US11344000B1 (en) | 2020-12-22 | 2022-05-31 | Monsanto Technology Llc | Cotton variety 19R113B3XF |
US11432521B2 (en) | 2020-12-22 | 2022-09-06 | Monsanto Technology Llc | Cotton variety 19R242NRB3XF |
US11317592B1 (en) | 2020-12-22 | 2022-05-03 | Monsanto Technology Llc | Cotton variety 19R238NRB3XF |
US11432520B2 (en) | 2020-12-22 | 2022-09-06 | Monsanto Technology Llc | Cotton variety 19R228B3XF |
EP4026833A1 (en) | 2021-01-12 | 2022-07-13 | Bayer Aktiengesellschaft | Herbicidally active 2-(het)arylmethyl pyrimidines |
US11602115B2 (en) | 2021-02-24 | 2023-03-14 | Monsanto Technology | Cotton variety 19R233B3XF |
US11606927B2 (en) | 2021-02-24 | 2023-03-21 | Monsanto Technology Llc | Cotton variety 19R130B3XF |
US11737420B2 (en) | 2021-02-24 | 2023-08-29 | Monsanto Technology Llc | Cotton variety 19R241NRB3XF |
US11589545B2 (en) | 2021-02-24 | 2023-02-28 | Monsanto Technology Llc | Cotton variety 19R240B3XF |
US11602116B2 (en) | 2021-02-24 | 2023-03-14 | Monsanto Technology Llc | Cotton variety 19R236B3XF |
US11589544B2 (en) | 2021-02-24 | 2023-02-28 | Monsanto Technology Llc | Cotton variety 19R229B3XF |
US11602114B2 (en) | 2021-02-24 | 2023-03-14 | Monsanto Technology Llc | Cotton variety 19R107B3XF |
US11606928B2 (en) | 2021-02-24 | 2023-03-21 | Monsanto Technology Llc | Cotton variety 19R231B3XF |
US11432522B1 (en) | 2021-02-24 | 2022-09-06 | Monsanto Technology Llc | Cotton variety 19R244B3XF |
US11617335B2 (en) | 2021-02-26 | 2023-04-04 | Monsanto Technology Llc | Cotton variety 19R249B3XF |
US11432523B1 (en) | 2021-02-26 | 2022-09-06 | Monsanto Technology Llc | Cotton variety 19R125B3XF |
US11617336B2 (en) | 2021-02-26 | 2023-04-04 | Monsanto Technology Llc | Cotton variety 19R254B3XF |
US11602117B2 (en) | 2021-02-26 | 2023-03-14 | Monsanto Technology Llc | Cotton variety 19R245B3XF |
US11497188B2 (en) | 2021-02-26 | 2022-11-15 | Monsanto Technology Llc | Cotton variety 19R132B3XF |
BR112023016920A2 (en) | 2021-03-12 | 2023-10-10 | Bayer Ag | CHIRAL N-(1,3,4-OXADIAZOLE-2-YL)PHENYL CARBOXYLIC ACID AMIDES AND THEIR USE AS HERBICIDES |
TW202304919A (en) | 2021-03-31 | 2023-02-01 | 印度商皮埃企業有限公司 | Fused heterocyclic compounds and their use as pest control agents |
CN115336534A (en) | 2021-05-12 | 2022-11-15 | 北京大北农生物技术有限公司 | Use of protoporphyrinogen oxidase |
CA3219711A1 (en) | 2021-05-26 | 2022-12-01 | Stephen P. Long | C4 plants with increased photosynthetic efficiency |
WO2022253700A1 (en) | 2021-06-01 | 2022-12-08 | Bayer Aktiengesellschaft | Specifically substituted pyrroline-2-ones and their use as herbicides |
IL309609A (en) | 2021-06-25 | 2024-02-01 | Bayer Ag | (1,4,5-trisubstituted-1h-pyrazole-3-yl)oxy-2-alkoxy alkyl acids and their derivatives, their salts and their use as herbicidal agents |
WO2023274869A1 (en) | 2021-06-29 | 2023-01-05 | Bayer Aktiengesellschaft | 3-(4-alkenyl-phenyl)-3-pyrrolino-2-ones and their use as herbicides |
CA3225637A1 (en) | 2021-07-02 | 2023-01-05 | Bayer Aktiengesellschaft | Herbicidal compositions containing cinmethyline and ethofumesate |
AR126252A1 (en) | 2021-07-08 | 2023-10-04 | Bayer Ag | SUBSTITUTED BENZOIC ACID AMIDES |
CN117794357A (en) | 2021-07-23 | 2024-03-29 | 巴斯夫农业种子解决方案美国有限责任公司 | Black shank-resistant plants and methods for identifying black shank-resistant plants |
CN117897378A (en) | 2021-09-03 | 2024-04-16 | 巴斯夫农业种子解决方案美国有限责任公司 | Plants with increased tolerance to herbicides |
AR126996A1 (en) | 2021-09-08 | 2023-12-06 | Pi Industries Ltd | ISOXAZOLINE COMPOUNDS AND THEIR USE AS PEST CONTROL AGENTS |
TW202328089A (en) | 2021-09-08 | 2023-07-16 | 印度商皮埃企業有限公司 | Sulfoximines/sulfilimine containing aromatic caboxamide compounds and their use therof |
WO2023044364A1 (en) | 2021-09-15 | 2023-03-23 | Enko Chem, Inc. | Protoporphyrinogen oxidase inhibitors |
WO2023052562A1 (en) | 2021-10-01 | 2023-04-06 | Basf Se | Wheat plants with an increased yield |
AU2022358470A1 (en) | 2021-10-01 | 2024-04-11 | Basf Se | Plants with improved properties |
WO2023084473A1 (en) | 2021-11-15 | 2023-05-19 | Pi Industries Ltd. | Bicyclic heteroaromatic compounds and their use as pest control agents |
WO2023099381A1 (en) | 2021-12-01 | 2023-06-08 | Bayer Aktiengesellschaft | (1,4,5-trisubstituted-1h-pyrazole-3-yl)oxy-2-alkoxythio alkyl acids and derivatives thereof, their salts and their use as herbicidal active agents |
WO2023137309A2 (en) | 2022-01-14 | 2023-07-20 | Enko Chem, Inc. | Protoporphyrinogen oxidase inhibitors |
US12004476B2 (en) | 2022-01-26 | 2024-06-11 | Monsanto Technology Llc. | Cotton variety 20R750B3XF |
US11985950B2 (en) | 2022-01-26 | 2024-05-21 | Monsanto Technology Llc | Cotton variety 19R114B3XF |
WO2023154887A1 (en) | 2022-02-11 | 2023-08-17 | Northeast Agricultural University | Methods and compositions for increasing protein and/or oil content and modifying oil profile in a plant |
TW202342756A (en) | 2022-03-01 | 2023-11-01 | 美商巴斯夫農業解決方案種子美國有限責任公司 | Cas12a nickases |
US20230323480A1 (en) | 2022-04-11 | 2023-10-12 | The Regents Of The University Of California | Methods of screening for plant gain of function mutations and compositions therefor |
WO2023218484A1 (en) | 2022-05-11 | 2023-11-16 | Pi Industries Ltd. | Bicyclic compounds and their use as pest control agents |
WO2023222226A1 (en) | 2022-05-19 | 2023-11-23 | Evonik Operations Gmbh | Enzymatic method for producing l-glufosinate |
WO2023222227A1 (en) | 2022-05-19 | 2023-11-23 | Evonik Operations Gmbh | Enzymatic method for producing l-glufosinate |
WO2024020360A1 (en) | 2022-07-18 | 2024-01-25 | Pairwise Plants Services, Inc. | Mustard green plants named 'pwrg-1', 'pwrg-2,' and 'pwsgc' |
WO2024041926A1 (en) | 2022-08-25 | 2024-02-29 | Bayer Aktiengesellschaft | Herbicidal compositions |
WO2024041925A1 (en) | 2022-08-25 | 2024-02-29 | Bayer Aktiengesellschaft | Herbicidal compositions |
WO2024078871A1 (en) | 2022-10-14 | 2024-04-18 | Bayer Aktiengesellschaft | 1-pyridyl-5-phenylpyrazolyl-3-oxy- and -3-thioalkyl acids and derivatives and their use for controlling undesired plant growth |
WO2024099765A2 (en) | 2022-11-10 | 2024-05-16 | BASF Agricultural Solutions Seed US LLC | Transcription regulating nucleotide sequences and methods of use |
US20240200085A1 (en) | 2022-12-15 | 2024-06-20 | Aarhus Universitet | Synthetic activation of multimeric transmembrane receptors |
Citations (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2007976A (en) | 1977-11-08 | 1979-05-31 | Meiji Seika Kaisha | Herbicidal compositions and herbicidal processes |
US4407956A (en) | 1981-03-13 | 1983-10-04 | The Regents Of The University Of California | Cloned cauliflower mosaic virus DNA as a plant vehicle |
US4459355A (en) | 1982-07-12 | 1984-07-10 | International Paper Company | Method for transforming plant cells |
WO1984002913A1 (en) | 1983-01-17 | 1984-08-02 | Monsanto Co | Chimeric genes suitable for expression in plant cells |
WO1984002920A1 (en) | 1983-01-17 | 1984-08-02 | Monsanto Co | Genetically transformed plants |
EP0173327A2 (en) | 1984-08-30 | 1986-03-05 | Meiji Seika Kabushiki Kaisha | Bialaphos producing gene |
WO1986002097A1 (en) | 1984-10-01 | 1986-04-10 | The General Hospital Corporation | Plant cells resistant to herbicidal glutamine synthetase inhibitors |
EP0196375A1 (en) | 1985-03-28 | 1986-10-08 | Meiji Seika Kabushiki Kaisha | Gene coding for signal peptides and utilization thereof |
US4769061A (en) | 1983-01-05 | 1988-09-06 | Calgene Inc. | Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase, production and use |
US5273894A (en) | 1986-08-23 | 1993-12-28 | Hoechst Aktiengesellschaft | Phosphinothricin-resistance gene, and its use |
US5276268A (en) | 1986-08-23 | 1994-01-04 | Hoechst Aktiengesellschaft | Phosphinothricin-resistance gene, and its use |
US5637489A (en) | 1986-08-23 | 1997-06-10 | Hoechst Aktiengesellschaft | Phosphinothricin-resistance gene, and its use |
US5648477A (en) | 1986-03-11 | 1997-07-15 | Plant Genetic Systems, N.V. | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0185005B1 (en) * | 1984-12-10 | 1992-01-22 | Monsanto Company | Insertion of the bacillus thuringiensis crystal protein gene into plant-colonizing microorganisms and their use |
AU590597B2 (en) * | 1985-08-07 | 1989-11-09 | Monsanto Technology Llc | Glyphosate-resistant plants |
US4795855A (en) * | 1985-11-14 | 1989-01-03 | Joanne Fillatti | Transformation and foreign gene expression with woody species |
ES2038631T3 (en) * | 1986-08-23 | 1993-08-01 | Hoechst Aktiengesellschaft | PROCEDURE FOR OBTAINING A RESISTANCE GENE AGAINST PHOSPHINOTRICIN (PTC). |
-
1987
- 1987-01-21 EP EP87400141A patent/EP0242236B2/en not_active Expired - Lifetime
- 1987-01-21 AT AT87400141T patent/ATE57390T1/en not_active IP Right Cessation
- 1987-01-21 ES ES87400141T patent/ES2018274T5/en not_active Expired - Lifetime
- 1987-01-21 DE DE8787400141T patent/DE3765449D1/en not_active Expired - Lifetime
- 1987-03-10 IL IL8183887A patent/IL81838A/en not_active IP Right Cessation
- 1987-03-11 ZA ZA871754A patent/ZA871754B/en unknown
- 1987-03-11 AU AU71673/87A patent/AU612570B2/en not_active Expired
- 1987-03-11 PT PT84448A patent/PT84448B/en unknown
- 1987-03-11 HU HU872100A patent/HU213580B/en unknown
- 1987-03-11 EP EP87400544A patent/EP0242246B1/en not_active Expired - Lifetime
- 1987-03-11 AT AT87400544T patent/ATE82323T1/en not_active IP Right Cessation
- 1987-03-11 DE DE8787400544T patent/DE3782526T2/en not_active Expired - Lifetime
- 1987-03-11 JP JP62502222A patent/JP3142848B2/en not_active Expired - Lifetime
- 1987-03-11 BR BR8706204A patent/BR8706204A/en unknown
- 1987-03-11 WO PCT/EP1987/000141 patent/WO1987005629A1/en active Application Filing
- 1987-03-11 ES ES87400544T patent/ES2052588T3/en not_active Expired - Lifetime
- 1987-11-04 FI FI874883A patent/FI874883A0/en not_active IP Right Cessation
- 1987-11-06 OA OA59219A patent/OA08771A/en unknown
- 1987-11-10 NO NO874673A patent/NO874673D0/en unknown
- 1987-11-10 DK DK198705898A patent/DK175656B1/en not_active IP Right Cessation
- 1987-11-11 KR KR1019870701032A patent/KR880701281A/en not_active Application Discontinuation
-
1990
- 1990-05-17 US US07/525,300 patent/US5561236A/en not_active Expired - Lifetime
- 1990-12-20 GR GR90401090T patent/GR3001220T3/en unknown
-
1993
- 1993-01-28 GR GR930400183T patent/GR3006936T3/el unknown
-
1995
- 1995-06-05 US US08/463,241 patent/US5646024A/en not_active Expired - Lifetime
- 1995-06-05 US US08/465,219 patent/US7112665B1/en not_active Ceased
- 1995-06-07 US US08/477,320 patent/US5648477A/en not_active Expired - Lifetime
-
1996
- 1996-10-16 GR GR960402753T patent/GR3021380T3/en unknown
-
1997
- 1997-11-06 HK HK97102114A patent/HK1000519A1/en not_active IP Right Cessation
-
1998
- 1998-11-24 JP JP33286298A patent/JP3483785B2/en not_active Expired - Lifetime
-
1999
- 1999-01-25 DK DK199900090A patent/DK175642B1/en not_active IP Right Cessation
-
2004
- 2004-06-15 CL CL200401483A patent/CL2004001483A1/en unknown
-
2013
- 2013-09-03 US US14/017,108 patent/USRE44962E1/en not_active Expired - Lifetime
Patent Citations (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB2007976A (en) | 1977-11-08 | 1979-05-31 | Meiji Seika Kaisha | Herbicidal compositions and herbicidal processes |
US4407956A (en) | 1981-03-13 | 1983-10-04 | The Regents Of The University Of California | Cloned cauliflower mosaic virus DNA as a plant vehicle |
US4459355A (en) | 1982-07-12 | 1984-07-10 | International Paper Company | Method for transforming plant cells |
US4769061A (en) | 1983-01-05 | 1988-09-06 | Calgene Inc. | Inhibition resistant 5-enolpyruvyl-3-phosphoshikimate synthase, production and use |
WO1984002913A1 (en) | 1983-01-17 | 1984-08-02 | Monsanto Co | Chimeric genes suitable for expression in plant cells |
WO1984002920A1 (en) | 1983-01-17 | 1984-08-02 | Monsanto Co | Genetically transformed plants |
EP0173327A2 (en) | 1984-08-30 | 1986-03-05 | Meiji Seika Kabushiki Kaisha | Bialaphos producing gene |
WO1986002097A1 (en) | 1984-10-01 | 1986-04-10 | The General Hospital Corporation | Plant cells resistant to herbicidal glutamine synthetase inhibitors |
EP0196375A1 (en) | 1985-03-28 | 1986-10-08 | Meiji Seika Kabushiki Kaisha | Gene coding for signal peptides and utilization thereof |
US5648477A (en) | 1986-03-11 | 1997-07-15 | Plant Genetic Systems, N.V. | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants |
US5273894A (en) | 1986-08-23 | 1993-12-28 | Hoechst Aktiengesellschaft | Phosphinothricin-resistance gene, and its use |
US5276268A (en) | 1986-08-23 | 1994-01-04 | Hoechst Aktiengesellschaft | Phosphinothricin-resistance gene, and its use |
US5637489A (en) | 1986-08-23 | 1997-06-10 | Hoechst Aktiengesellschaft | Phosphinothricin-resistance gene, and its use |
US5879903A (en) | 1986-08-23 | 1999-03-09 | Hoechst Aktiengesellschaft | Phosphinothricin-resistance gene, and its use |
Non-Patent Citations (48)
Title |
---|
"Report On The Filing Or Determination Of An Action Regarding A Patent Or Trademark", U.S. District Court in the Eastern District of Virginia; Case No. 2:12cv46, dated Jan. 25, 2012, 2 pages. |
Bayer et al., Helvetica Chimica Acta, vol. 55, No. 25, pp. 224-239 (1972). |
Bowie et al 1990, Science 247:1306-1310. |
Browun et al 1998, Science 282:1315-1317. |
Cashmore, Proc. Natl. Acad. Sci. USA, vol. 81, pp. 2960-2964 (1984). |
Comai et al 1985, Nature 317:741-744. |
Coruzzi et al. "Nucleotide Sequences of Two Pea cDNA Clones Encoding the Small Subunit of Riboluse 1,5-Bisphosphate Carboxylase . . . ", Journal of Biological Chem. vol. 258, No. 3, Feb. 10, 1983, pp. 1399-1402. |
Cullimore et al., "Purification and Properties of two forms of glutamine synthetase from the plant fraction of Phaseolus root nodules", Planta Journal, 1983, 157: 245-253. |
De Block et al., "Engineering herbicide resistance in plants by expression of a detoxifying enzyme", EMBO Journal vol. 6, No. 9, 1987, pp. 2513-2518. |
De Cleene, M., "The Susceptibility of Monocotyledons to Agrobacterium tumefaciens", Pythopathology, 1985, 113: 81-89. |
Donn et al., "Herbicide-Resistant Alfalfa Cells: An Example of Gene Amplification in Plants", Journal of Molecular and Applied Genetics, 1984, vol. 2, No. 6, pp. 621-635. |
Donn et al., Chem Abstracts, 1985, vol. 102, No. 144025J: 140. |
E. Strauch et al., "Cloning of a Phosphinothricin N-acetyltransferase Gene from Streptomyces viridochromogenes Tu494 and its Expression in Streptomyces lividans and Escherichia coli", Gene, 63:65-74 (1988), Elsevier Science, Oxford, United Kingdom. |
Erickson et al., Chemical Abstracts, vol. 104, No. 9, p. 311, 64619g (Mar. 3, 1986). |
Fayerman et al., Biotechnology, vol. 4, No. 9, pp. 786-789 (Sep. 1986). |
Goodman et al., "Gene Transfer in Crop Improvement", Science, Apr. 3, 1987, vol. 236, No. 4797, pp. 48-54. |
Hill et al 1998, Biochem. Biophys. Res. Comm. 244:573-577. |
Jones et al., Chemical Abstracts, vol. 104, No. 5, p. 152, 29747a (Feb. 1986). |
Journal of Cellular Biochemistry, Abstracts, Mar. 31-Apr. 29, 1984, Alan R. Liss, Inc., New York. |
Kobayashi et al., The Journal of Antibiotics, vol. 49, No. 5, pp. 688-693 (May 1986). |
Langelueddeke et al. Chemical Abstracts, vol. 98, p. 242, 48585v (1982). |
Larkin et al., "Somaclonal Variation-a Novel Source of Variability from Cell Cultures for Plant Improvement", Journal for Molecular Appl Genet. vol. 60, 1981, pp. 197-214. |
Lazar et al., "Transforming Growth Factor: Mutation pf Aspartic Acid 47 and Leucine 48 Results in Different Biological Activities", Molecular and Cellular Biology, vol. 8, Mar. 1988, pp. 1247-1252. |
Leason et al., "Inhibition of Pea Leaf Glutamine Synthetase by Methionine Sulphoximine, Phosphinothricin and Other Glutamate Analogues", Phytochemistry, vol. 21, No. 4, pp. 855-857, 1982. |
Mason et al., Phytochemistry, vol. 21, No. 4, pp. 855-857 (1982). |
Murakami et al., "The bialaphos biosynthetic genes of Streptomyces hygroscopicus: Molecular cloning and characterization of the gene cluster", Mol Gen Genet. vol. 205, 1986, pp. 42-50. |
Murakami et al., Chemical Abstracts, vol. 106, p. 1149, 1151u (1987). |
Sanders et al., "Amplification and cloning of the Chinese hamster glutamine synthetase gene", The EMBO Journal, vol. 3, No. 1, pp. 65-71, (1984). |
Schreier et al. EMBO J., vol. 4, pp. 25-32, (1985). |
Scolnik et al., "The Wild-Type Gene for Glutamine Synthetase Restores Ammonia Control of the Nitrogen Fixation . . . ", Journal of Bacteriology, vol. 155, No. 1, Jul. 1983, pp. 180-185. |
Shields, "Engineering Herbicide Resistance", Nature, vol. 317, Oct. 1985, p. 668. |
Stalker et al., "Herbicide Resistance in Transgenic Plants Expressing a Bacterial Detoxification Gene", Science, Oct. 21, 1988, pp. 419-423. |
T. W. Goodwin and E. I. Mercer, "Introduction to Plant Biochemistry", 1983, 2nd ed., pp. 349-353. |
Thompson (Sep. 3-8, 1984) Genetic Engineering and Antibiotic Production, Rev. Biol., vol. 77, No. 4, pp. 567-568. |
Thompson et al 1987, The EMBO Journal 6(9):2519-2523. |
Thompson et al., Journal of Bacteriology, vol. 151, No. 2, pp. 678-685 (1982). |
Thompson et al., Mol. Gen. Genet., vol. 195, pp. 39-43 (1984). |
Thompson et al., Nature, vol. 286, No. 5772, pp. 525-527 (1980). |
Thompson et al., Proc. Natl. Acad. Sci. USA, vol. 80, pp. 5190-5194 (1983). |
Thompson, et al., Gene, vol. 20, pp. 51-62 (1982). |
Vara et al., "Cloning and expression of a puromycin N-acetyl transferase gene from Streptomyces alboniger in Streptomyces lividans and Escherichia coli", Gene, vol. 33, No. 2, 1985, pp. 197-206. |
Vasil, "Progress In The Regeneration and Genetic Manipulation of Cereal Crops", Biotechnology vol. 6, Apr. 1988, pp. 397-402. |
Velten et al. Nucleic Acids Research, vol. 13, No. 19, pp. 6981-6998 (1985). |
W. Wohlleben et al., "Nucleotide Sequence of the Phosphinothricin N-acetyltransferase Gene from Streptomyces viridochromogenes Tu 494 and its Expression in Nicotiana tabacum", Gene, 70:25-37 (1988), Elsevier Science, Oxford, United Kingdom. |
Wedler et al., "Interaction of a new y-glutamyl-phosphate analog, 4-(phosphonoacetyl) . . . ", Archives of Biochemistry and Biophysics, vol. 202, No. 2, Jul. 1980, pp. 482-490. |
Winnacker, Ernst-L, "From Genes to Clones: Introduction to Gene Technology", 1987, pp. 413-415. |
Worthing (Ed.), The Pesticide Manual, 7th Ed., p. 302, 1983. |
Zalacain et al. Nucleic Acids Research, vol. 14, No. 4, pp. 1565-1581 (Feb. 1986). |
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9992943B2 (en) | 2016-04-11 | 2018-06-12 | Bayer Cropscience Lp | Cotton variety ST 4949GLT |
US10010041B2 (en) | 2016-04-11 | 2018-07-03 | Bayer Cropscience Lp | Cotton variety ST 4848GLT |
US10448611B2 (en) | 2016-04-11 | 2019-10-22 | Basf Agricultural Solutions Seed, Us Llc | Cotton variety FM 1911GLT |
US10827718B2 (en) | 2017-02-13 | 2020-11-10 | Basf Agricultural Solutions Seed, Us Llc | Cotton variety ST 5020GLT |
US10874082B2 (en) | 2017-02-13 | 2020-12-29 | Basf Agricultural Solutions Seed, Us Llc | Cotton variety FM 1953GLTP |
US10470428B2 (en) | 2018-03-07 | 2019-11-12 | Basf Agricultural Solutions Seed, Us Llc | Cotton variety ST 5471GLTP |
US11076548B2 (en) | 2018-03-07 | 2021-08-03 | Basf Agricultural Solutions Seed, Us Llc | Cotton variety ST 5122GLT |
US11213003B2 (en) | 2019-02-12 | 2022-01-04 | BASF Agricultural Solutions Seed US LLC | Cotton variety ST 4550GLTP |
US11234407B2 (en) | 2019-02-12 | 2022-02-01 | BASF Agricultural Solutions Seed US LLC | Cotton variety FM 1621GL |
US11284595B2 (en) | 2019-02-12 | 2022-03-29 | BASF Agricultural Solutions Seed US LLC | Cotton variety FM 2398GLTP |
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
USRE44962E1 (en) | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments and recombinants for use in the production of said cells and plants | |
EP0554240B1 (en) | Expression of herbicide metabolizing cytochromes p450 | |
JP2511036B2 (en) | Glutathione S-transferase gene and herbicide-tolerant plant containing the gene | |
US5559024A (en) | Chimeric nitrilase-encoding gene for herbicidal resistance | |
CA2261094C (en) | Chimera gene with several herbicide resistant genes, plant cell and plant resistant to several herbicides | |
AU652610B2 (en) | Chimeric gene for the transformation of plants | |
US5073677A (en) | Herbicidal tolerant plants containing rat glutathione S-transferase gene | |
CA1341470C (en) | Genetically engineered plant cells resistant to glutamine synthetase inhibitors | |
CA1341531C (en) | Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibittors, dna fragments and recombinants for use in the production of said cells andplants | |
EP0805865A1 (en) | Deoxyribonucleic acid coding for glutathion-s-transferase and its use | |
IE83968B1 (en) | Transit peptide DNA sequence |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
RR | Request for reexamination filed |
Effective date: 20141107 |
|
RR | Request for reexamination filed |
Effective date: 20150601 |
|
RR | Request for reexamination filed |
Effective date: 20150807 |
|
MAFP | Maintenance fee payment |
Free format text: PAYMENT OF MAINTENANCE FEE, 12TH YEAR, LARGE ENTITY (ORIGINAL EVENT CODE: M1553) Year of fee payment: 12 |
|
CONR | Reexamination decision confirms claims |
Kind code of ref document: C1 Free format text: REEXAMINATION CERTIFICATE Filing date: 20141107 Effective date: 20210212 |
|
CC | Certificate of correction | ||
CC | Certificate of correction | ||
CC | Certificate of correction |