US7153955B2 - Pentopyranosyl nucleic acid arrays, and uses thereof - Google Patents
Pentopyranosyl nucleic acid arrays, and uses thereof Download PDFInfo
- Publication number
- US7153955B2 US7153955B2 US10/150,402 US15040202A US7153955B2 US 7153955 B2 US7153955 B2 US 7153955B2 US 15040202 A US15040202 A US 15040202A US 7153955 B2 US7153955 B2 US 7153955B2
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- Prior art keywords
- mmol
- benzoyl
- mixture
- solution
- ribopyranosyl
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Definitions
- the present invention relates to a pentopyranosylnucleoside of the formula (I) or of the formula (II)
- p-NAs Pyranosylnucleic acids
- p-NAs are in general structural types which are isomeric to the natural RNA, in which the pentose units are present in the pyranose form and are repetitively linked by phosphodiester groups between the positions C-2′ and C-4′ ( FIG. 1 ).
- Nucleobase is understood here as meaning the canonical nucleobases A, T, U, C, G, but also the pairs isoguanine/isocytosine and 2,6-diaminopurine/xanthine and, within the meaning of the present invention, also other purines and pyrimidines.
- p-NAs namely the p-RNAs derived from ribose
- Eschenmoser et al. see Pitsch, S. et al. Helv. Chim. Acta 1993, 76, 2161; Pitsch, S. et al. Helv. Chim Acta 1995, 78, 1621; Angew. Chem. 1996, 108, 1619–1623). They exclusively form so-called Watson-Crick-paired, i.e. purine-pyrimidine- and purine-purine-paired, antiparallel, reversibly “melting”, quasilinear and stable duplexes.
- Homochiral p-RNA strands of the opposite sense of chirality likewise pair controllably and are strictly non-helical in the duplex formed.
- This specificity which is valuable for the construction of supramolecular units, is associated with the relatively low flexibility of the ribopyranose phosphate backbone and with the strong inclination of the base plane to the strand axis and the tendency resulting from this for intercatenary base stacking in the resulting duplex and can finally be attributed to the participation of a 2′,4′-cis-disubstituted ribopyranose ring in the construction of the backbone.
- p-NAs pairing systems form a pairing system which is orthogonal to natural nucleic acids, i.e. they do not pair with the DNAs and RNAs occurring in the natural form, which is of importance, in particular, in the diagnostic field.
- Eschenmoser et al. (1993, supra) has for the first time prepared a p-RNA, as shown in FIG. 2 and illustrated below.
- a suitable protected nucleobase was reacted with the anomer mixture of the tetrabenzoylribopyranose by action of bis(trimethylsilyl)acetamide and of a Lewis acid such as, for example, trimethylsilyl trifluoromethanesulphonate (analogously to H. Vorbrüggen, K. Krolikiewicz, B. Bennua, Chem. Ber. 1981, 114, 1234).
- the carrier-bonded component in the 4′-position was repeatedly acidically deprotected, a phosphoramidite was coupled on under the action of a coupling reagent, e.g. a tetrazole derivative, still free 4′-oxygen atoms were acetylated and the phosphorus atom was oxidized in order thus to obtain the oligomeric product.
- a coupling reagent e.g. a tetrazole derivative
- a biomolecule e.g. DNA or RNA
- Biomolecules of this type are used, for example, in analytical systems for signal amplification, where a DNA molecule whose sequence is to be analysed is on the one hand to be immobilized by means of such a non-covalent DNA linker on a solid support, and on the other hand is to be bonded to a signal-amplifying branched DNA molecule (bDNA)(see, for example, S. Urdea, Biol/Technol.
- bDNA signal-amplifying branched DNA molecule
- the object of the present invention was therefore to provide novel biomolecules and a process for their preparation in which the above-described disadvantages can be avoided.
- p-NAs as an orthogonal pairing system which does not intervene in the DNA or RNA pairing process solves this problem advantageously, as a result of which the sensitivity of the analytical processes described can be markedly increased.
- One subject of the present invention is therefore the use of pentopyranosylnucleotides or pentopyranosylnucleic acids preferably in the form of a conjugate comprising a pentopyranosylnucleotide or a pentopyranosylnucleic acid and a biomolecule for the production of an electronic component, in particular in the form of a diagnostic.
- Conjugates within the meaning of the present invention are covalently bonded hybrids of p-NAs and other biomolecules, preferably a peptide, protein or a nucleic acid, for example an antibody or a functional moiety thereof or a DNA and/or RNA occurring in its natural form.
- Functional moieties of antibodies are, for example, Fv fragments (Skerra & Plückthun (1988) Science 240, 1038), single-chain Fv fragments (scFv; Bird et al. (1988), Science 242, 423; Huston et al. (1988) Proc. Natl. Acad. Sci. USA, 85, 5879) or Fab fragments (Better et al. (1988) Science 240, 1041).
- Biomolecule within the meaning of the present invention is understood as meaning a naturally occurring substance or a substance derived from a naturally occurring substance.
- they are in this case p-RNA/DNA or p-RNA/RNA conjugates.
- Conjugates are preferably used when the functions “sequence recognition” and “non-covalent bonding” must be realized in a molecule, since the conjugates according to the invention contain two pairing systems which are orthogonal to one another.
- p-NAs and in particular the p-RNAs form stable duplexes with one another and in general do not pair with the DNAs and RNAs occurring in their natural form. This property makes p-NAs preferred pairing systems.
- Such pairing systems are supramolecular systems of non-covalent interaction, which are distinguished by selectivity, stability and reversibility, and their properties are preferably influenced thermodynamically, i.e. by temperature, pH and concentration.
- such pairing systems can also be used, for example, as “molecular adhesive” for the bringing together of different metal clusters to give cluster associations having potentially novel properties [see, for example, R. L. Letsinger, et al., Nature 1996, 382, 607–9; P. G. Schultz et al., Nature 1996, 382, 609–11].
- the p-NAs are also suitable for use in the field of nanotechnology, for example for the production of novel materials, diagnostics and therapeutics and also microelectronic, photonic or optoelectronic components and for the controlled bringing together of molecular species to give supramolecular units, such as, for example, for the (combinatorial) synthesis of protein assemblies [see, for example, A. Lombardi, J. W. Bryson, W. F. DeGrado, Biomoleküls (Pept. Sci.) 1997, 40, 495–504], as p-NAs form pairing systems which are strongly and thermodynamically controllable.
- a further application therefore especially arises in the diagnostic and drug discovery field due to the possibility of providing functional, preferably biological units, such as proteins or DNA/RNA sections, with a p-NA code which does not interfere with the natural nucleic acids (see, for example, WO 93/20242).
- a DNA oligonucleotide for example, is additionally synthesized. This process can also be carried out in the reverse sequence.
- p-RNA oligomers having amino-terminal linkers and, for example, DNA oligomers having, for example, thiol linkers are synthesized in separate operations.
- An iodoacetylation of the p-RNA oligomer and the coupling of the two units according to protocols known from the literature (T. Zhu et al., Bioconjug. Chem. 1994, 5, 312) is then preferably carried. out.
- Convergent processes prove to be particularly preferred on account of their flexibility.
- arrays are arrangements of immobilized recognition species which, especially in analysis and diagnosis, play an important role in the simultaneous determination of analytes. Examples are peptide arrays (Fodor et al., Nature 1993, 364, 555) and nucleic acid arrays (Southern et al. Genomics 1992, 13, 1008; Heller, U.S. Pat. No. 5,632,957). A higher flexibility of these arrays can be achieved by binding the recognition species to coding oligonucleotides and the associated, complementary strands to certain positions on a solid carrier.
- the recognition species are non-covalently bonded to the desired positions.
- various types of recognition species such as, for example, DNA sections, antibodies, can only be arranged simultaneously on a solid carrier by use of hybridization conditions (see FIG. 3 ).
- the prerequisite for this, however, are codons and anticodons which are extremely strong and selective—in order to keep the coding sections as short as possible—and do not interfere with natural nucleic acid necessary.
- p-NAs preferably p-RNAs, are particularly advantageously suitable for this.
- carrier within the meaning of the present invention is understood as meaning material, in particular chip material, which is present in solid or alternatively gelatinous form.
- Suitable carrier materials are, for example, ceramic, metal, in particular noble metal, glasses, plastics, crystalline materials or thin layers of the carrier, in particular of the materials mentioned, or (bio)molecular filaments such as cellulose, structural proteins.
- the present invention therefore also relates to the use of pentopyranosylnucleic acids, preferably ribopyranosylnucleic acids for encoding recognition species, preferably natural DNA or RNA strands or proteins, in particular antibodies or functional moieties of antibodies. These can then be hybridized with the appropriate codons on a solid carrier according to FIG. 3 .
- recognition species preferably natural DNA or RNA strands or proteins, in particular antibodies or functional moieties of antibodies.
- the species to be detected are bonded to the array in a certain pattern which is then recorded indirectly (e.g. by fluorescence labelling of the recognition species) or directly (e.g. by impedance measurement at the linkage point of the codon).
- the hybridization is then eliminated by suitable conditions (temperature, salts, solvents, electrophoretic processes) so that again only the carrier having the codons remains. This is then again loaded with other recognition species and is used, for example, for the same analyte for the determination of another sample.
- suitable conditions temperature, salts, solvents, electrophoretic processes
- the pentopyranosylnucleoside is a compound of the formula (I)
- R 12 , R 13 , R 14 and R 15 independently of one another, identically or differently, are in each case H, OR 7 , where R 7 has the abovementioned meaning, or C n H 2n+1 , or C n H 2n ⁇ 1 , where n has the abovementioned meaning, and
- R 1′ is equal to H, OH, Hal where Hal is equal to Br or Cl, or a radical selected from
- X′ is in each case ⁇ N—, ⁇ C(R 16′ )— or —N(R 17 )—, where R 16′ and R 17′ independently of one another have the abovementioned meaning of R 16 or R 17 , and S c1′ and S c2′ have the abovementioned meaning of S a1 and S c2 .
- the pentopyranosylnucleoside is in general a ribo-, arabino-, lyxo- and/or xylopyranosylnucleoside, preferably a ribopyranosylnucleoside, where the pentopyranosyl moiety can be in the D configuration, but also in the L configuration.
- the pentopyranosylnucleoside is a pentopyranosylpurine,-2,6-diaminopurine, -6-purinethiol, -pyridine, -pyrimidine, -adenosine, -guanosine, -isoguanosine, -6-thioguanosine, -xanthine, -hypoxanthine, -thymidine, -cytosine, -isocytosine, -indole, -tryptamine, -N-phthaloyltryptamine, -uracil, -caffeine, -theobromine, -theophylline, -benzotriazole or -acridine, in particular a pentopyranosylpurine, -pyrimidine, -adenosine, -guanosine, -thymidine, -cytosine, in particular a pentopyra
- the compounds also include pentopyranosylnucleosides which can be used as linkers, i.e. as compounds having functional groups which can bond covalently to biomolecules, such as, for example, nucleic acids occurring in their natural form or modified nucleic acids, such as DNA, RNA but also p-NAs, preferably pRNAs. This is surprising, as no linkers are yet known for p-NAs.
- these include pentopyranosylnucleosides in which R 2 , R 3 , R 4 , R 2′ , R 3′ and/or R 4′ is a 2-phthalimidoethyl or allyloxy radical.
- Preferred linkers according to the present invention are, for example, uracil-based linkers in which the 5-position of the uracil has preferably been modified, e.g. N-phthaloylaminoethyluracil, but also indole-based linkers, preferably tryptamine derivatives, such as, for example, N-phthaloyltryptamine.
- pentopyranosyl-N,N-diacylnucleosides preferably purines, in particular adenosine, guanosine or 6-thioguanosine, are also made available, whose nucleobase can be completely deprotected in a simple manner.
- the invention therefore also includes pentopyranosylnucleosides in which R 2 , R 3 , R 4 , R 2′ , R 3′ and/or R 4′ is a radical of the formula —N[C(O)R 9 ] 2 , in particular N 6 , N 6 -dibenzoyl-9-( ⁇ -D-ribopyranosyl)-adenosine.
- the present invention makes available pentopyranosylnucleosides which carry a protective group, preferably a protective group which can be removed by base or metal catalysis, in particular an acyl group, particularly preferably a benzoyl group, exclusively on the 3′-oxygen atom of the pentopyranoside moiety.
- a protective group preferably a protective group which can be removed by base or metal catalysis
- an acyl group particularly preferably a benzoyl group
- These compounds serve, for example, as starting substances for the direct introduction of a further protective group, preferably of an acid- or base-labile protective group, in particular of a trityl group, particularly preferably a dimethoxytrityl group, onto the 4′-oxygen atom of the pentopyranoside moiety without additional steps which reduce the yield, such as, for example, additional purification steps.
- the present invention makes available pentopyranosylnucleosides which carry a protective group, preferably an acid- or base-labile protective group, in particular a trityl group, particularly preferably a dimethoxytrityl group, exclusively on the 4′-oxygen atom of the pentopyranoside moiety.
- a protective group preferably an acid- or base-labile protective group, in particular a trityl group, particularly preferably a dimethoxytrityl group, exclusively on the 4′-oxygen atom of the pentopyranoside moiety.
- a further protective group preferably of a protective group which can be removed by base or metal catalysis
- an acyl group particularly preferably of a benzoyl group, e.g. on the 2′-oxygen atom of the pentopyranoside moiety, without additional steps which reduce the yield, such as, for example, additional purification steps.
- the pentopyranosidenucleosides according to the invention can be reacted in a so-called one-pot reaction, which increases the yields and is therefore particularly advantageous.
- Suitable precursors for the oligonucleotide synthesis are, for example, 4′-DMT-pentopyranosylnucleoside-2′-phosphitamide/-H-phosphonate, preferably a 4′DMT-ribopyranosylnucleoside-2′-phosphitamide/-H-phosphonate, in particular a 4′-DMT-ribopyranosyladenine-, -guanine-, -cytosine-, -thymidine-, -xanthine-, hypoxanthine-, or -uracil-2′-phosphitamide/-H-phosphonate and an N-benzoyl-4′-DMT-ribopyranosyladenine-, -guanine- or -cytosine-2′-phosphitamide/-H-phosphonate and an N-isobutylroyl-4′-DMT-ribopyranosyladenine-, -guanine-
- the pentapyranosylnucleosides can be particularly advantageously prepared according to the present invention in that, starting from the unprotected pentopyranoside,
- a rearrangement of the protective group from the 2′-position to the 3′-position takes place, which in general is carried out in the presence of a base, in particular in the presence of N-ethyldiisopropylamine and/or triethylamine.
- this reaction can be carried out particularly advantageously in the same reaction container as the one-pot reaction.
- the pyranosylnucleoside is protected by a protective group S c1 , S c2 , S c1′ or S c2′ which is acid-labile, base-labile or can be removed with metal catalysis, the protective groups S c1 and S c1′ preferably being different from the protective groups S c2 and S c2′ .
- the protective groups mentioned are an acyl group, preferably an acetyl, benzoyl, nitrobenzoyl and/or methoxybenzoyl group, trityl groups, preferably a 4,4′-dimethoxytrityl (DMT) group or a ⁇ -eliminable group, preferably a group of the formula —OCH 2 CH 2 R 18 where R 18 is equal to a cyano or p-nitrophenyl radical or a fluorenylmethyloxycarbonyl (Fmoc) group.
- acyl group preferably an acetyl, benzoyl, nitrobenzoyl and/or methoxybenzoyl group
- trityl groups preferably a 4,4′-dimethoxytrityl (DMT) group or a ⁇ -eliminable group, preferably a group of the formula —OCH 2 CH 2 R 18 where R 18 is equal to a cyano or p-nitropheny
- the 2′- or 3′-position is protected by a protective group which is base-labile or can be removed with metal catalysis, preferably by an acyl group, in particular by an acetyl, benzoyl, nitrobenzoyl and/or methoxybenzoyl group, and/or the 4′-position is protected by an acid- or base-labile protective group, preferably by a trityl and/or Fmoc group, in particular by a DMT group.
- a protective group which is base-labile or can be removed with metal catalysis preferably by an acyl group, in particular by an acetyl, benzoyl, nitrobenzoyl and/or methoxybenzoyl group
- an acid- or base-labile protective group preferably by a trityl and/or Fmoc group, in particular by a DMT group.
- this process consequently manages without acetal protective groups, such as acetals or ketals, which avoids additional chromatographic intermediate purifications and consequently allows the reactions to be carried out as one-pot reactions with surprisingly high space/time yields.
- the protective groups mentioned are preferably introduced at low temperatures, as by this means they can be introduced surprisingly selectively.
- a benzoyl group takes place by reaction with benzoyl chloride in pyridine or in a pyridine/methylene chloride mixture at low temperatures.
- a DMT group can be introduced, for example, by reaction with DMTCl in the presence of a base, e.g. of N-ethyldiisopropylamine (Hünig's base), and, for example, of pyridine, methylene chloride or a pyridine/methylene chloride mixture at room temperature.
- reaction products are purified by chromatography. Purification after the tritylation is not necessary according to the process according to the invention, which is particularly advantageous.
- the final product if necessary, can additionally be further purified by crystallization.
- pentopyranoses such as, for example, tetrabenzoylpentopyranoses, preferably ⁇ -tetrabenzoylribopyranoses (R. Jeanloz, J. Am. Chem. Soc. 1948, 70, 4052).
- a linker according to formula (II), in which R 4′ is (C n H 2n )NR 10′ R 11′ and R 10′ R 11′ is linked by means of a radical of the formula (III) having the meaning already designated, is advantageously prepared by the following process:
- indole derivatives as linkers have the advantage of the ability to fluoresce and are therefore particularly preferred for nanotechnology applications in which it may be a matter of detecting very small amounts of substance.
- indole-1-ribosides have already been described in N. N. Suvorov et al., Biol. Aktivn. Soedin., Akad. Nauk SSSR 1965, 60 and Tetrahedron 1967, 23, 4653.
- there is no analogous process for preparing 3-substituted derivatives In general, their preparation takes place via the formation of an aminal of the unprotected sugar component and an indoline, which is then converted into the indole-1-riboside by oxidation.
- indole-1-glucosides and -1-arabinosides have been described (Y. V. Dobriynin et al. Khim.-Farm Zh. 1978, 12, 33), whose 3-substituted derivatives were usually prepared by means of Dahlsmeier's reaction.
- This route for the introduction of aminoethyl units into the 3-position of the indole is too complicated, however, for industrial application.
- a linker according to formula (I), in which X and Y independently of one another, identically or differently, are in each case ⁇ C(R 16 ) where R 16 is equal to H or C n H 2n and Z ⁇ C(R 16 )— where R 16 is equal to (C n H 2n )NR 10 R 11 is therefore advantageously prepared by the following process:
- the nucleosidation partner of the sugar used is preferably tryptamine, in particular N-acyl derivatives of tryptamine, especially N-phthaloyltryptamine.
- the 4′-protected, preferably, the 3′,4′-protected pentopyranosylnucleosides are phosphitylated in a further step or bonded to a solid phase.
- Phosphitylation is carried out, for example, by means of monoallyl N-diisopropylchlorophosphoramidite in the presence of a base, e.g. N-ethyldiisopropylamine or by means of phosphorus trichloride and imidazole or tetrazole and subsequent hydrolysis with addition of base.
- a base e.g. N-ethyldiisopropylamine
- the product is a phosphoramidite
- an H-phosphonate e.g. “long-chain alkylamino-controlled pore glass” (CPG, Sigma Chemie, Kunststoff)
- the compounds obtained serve, for example, for the preparation of pentopyranosylnucleic acids, in which, preferably
- Acidic activators such as pyridinium hydrochloride, preferably benzimidazolium triflate, are suitable as a coupling reagent when phosphoramidites are employed, preferably after recrystallizing in acetonitrile and after dissolving in acetonitrile, as in contrast to 5-(4-nitrophenyl)-1H-tetrazole as a coupling reagent no blockage of the coupling reagent lines and contamination of the product takes place.
- pyridinium hydrochloride preferably benzimidazolium triflate
- Arylsulphonyl chlorides, diphenyl chlorophosphate, pivaloyl chloride or adamantoyl chloride are particularly suitable as a coupling reagent when H-phosphonates are employed.
- a salt such as sodium chloride
- Allyloxy groups can preferably be removed by palladium [Pd(0)] complexes, e.g. before hyrazinolysis.
- pentofuranosylnucleosides e.g. adenosine, guanosine, cytidine, thymidine and/or uracil occurring in their natural form
- step (a) and/or step (b) can also be incorporated in step (a) and/or step (b), which leads, for example, to a mixed p-NA-DNA or p-NA-RNA.
- amino-terminal linkers can thus be synthesized which carry both an activatable phosphorus compound and an acid-labile protective group, such as DMT, and can therefore easily be used in automatable oligonucleotide synthesis (see, for example, P. S. Nelson et al., Nucleic Acid Res. 1989, 17, 7179; L. J. Arnold et al., WO 8902439).
- a further subject of the present invention also relates to an electronic component in particular in the form of a diagnostic, comprising an above-described pentopyranosylnucleoside or a pentopyranosylnucleoside in the form of a conjugate, and a process for the preparation of a conjugate, in which a pentopyranosylnucleoside or a pentopyranosylnucleic acid is combined with a biomolecule, as already described in detail above.
- FIG. 1 shows a section of the structure of RNA in its naturally occurring form (left) and in the form of a p-NA (right).
- FIG. 2 schematically shows the synthesis of a p-ribo(A,U)-oligonucleotide according to Eschenmoser et al (1993).
- FIG. 3 schematically shows an arrangement of immobilized recognition structures (arrays) on a solid carrier.
- DMTCl dimethoxytrityl chloride
- the reaction mixture was treated with 2.46 g (20.5 mmol; 0.1 eq.) of 4-dimethylaminopyridine (DMAP), cooled to ⁇ 6° C. and 27.9 ml (0.24 mol; 1.2 eq.) of benzoyl chloride (BzCl) in 30 ml of pyridine were added dropwise between ⁇ 6 and ⁇ 1° C. in the course of 15 min and the mixture was stirred for 10 min. To complete the reaction, a further 2.8 ml (24 mmol; 0.12 eq.) of BzCl were in each case added with cooling at an interval of 25 min and the mixture was finally stirred for 20 min.
- DMAP 4-dimethylaminopyridine
- BzCl benzoyl chloride
- the residue was taken up in 2 l of ethyl acetate, the molecular sieve was filtered off, the org. phase was extracted three times with 1 l of water each time and extracted once by stirring with 1.2 l of 10% strength citric acid and the org. phase was again separated off, extracted once with 1 l of water and finally with 1 l of saturated NaHCO 3 solution.
- the org. phase was dried using sodium sulphate, filtered and concentrated (220 g of residue).
- the residue was first filtered through silica gel 60 (20 ⁇ 10 cm) using a step gradient of heptane/ethyl acetate, 1:1 to 0.1) for prepurification, then chromatographed on silica gel 60 (30 ⁇ 10 cm; step gradient of dichloromethane/ethyl acetate, 1:0 to 1:1).
- N 4 -benzoyl-1-( ⁇ -D-ribopyranosyl)cytosine 1 were dissolved in 830 ml of dimethylformamide (DMF) and 1.5 l of pyridine (both solvents dried and stored over molecular sieve 3 ⁇ ) with warming to 124° C. 23.0 g (0.163 mol; 1.05 eq.) of BzCl, dissolved in 210 ml of pyridine, were added dropwise at ⁇ 58° to ⁇ 63° C. in the course of 3.5 h. The batch was stirred overnight in a cooling bath.
- DMF dimethylformamide
- pyridine both solvents dried and stored over molecular sieve 3 ⁇
- the G triol A (393 mg, 1.0 mmol) was dissolved in 4 ml of dry dichloromethane. The solution was treated with trimethyl orthobenzoate (0.52 ml, 3.0 mmol) and camphorsulphonic acid (58 mg, 0.25 mmol) and stirred for 15 h at room temperature. The mixture was then cooled to 0° C. and treated with 2 ml of mixture of acetonitrile, water and trifluoroacetic acid (50:5:1), which was precooled to 0° C. The mixture was stirred for 10 min and the solvent was removed in vacuo. The residue was purified by flash chromatography on silica gel (2.3 ⁇ 21 cm) using dichloromethane/methanol 100:3. 25 mg (5%) of 4-O-benzoyl compound 139 mg (28%) of mixed fractions and 205 mg (41%) of the desired 3-O-benzoyl compound B were obtained.
- the diol B (101 mg, 0.2 mmol) was suspended in 3.2 ml of dry dichloromethane.
- the suspension was treated with 171 ⁇ l (1.0 mmol) of N-ethyldiisopropylamine, 320 ⁇ l (3.96 mmol) of pyridine and 102 mg (0.3 mmol) of DMTCl and stirred at room temperature. After 24 h, a further 102 mg (0.3 mmol) of DMTCl were added and the mixture was again stirred for 24 h. It was then diluted with 30 ml of dichloromethane.
- hydroxyethyluracil 28 is possible on a large scale according to a known method (J. D. Fissekis, A. Myles, G. B. Brown, J. Org. Chem. 1964, 29, 2670).
- g-Butyrolactone 25 was formylated with methyl formate, the sodium salt 26 was reacted to give the urea derivative 27 and this was cyclized to give the hydroxyethyluracil 28 (Scheme 4).
- Hydroxyethyluracil 28 was mesylated with methanesulphonyl chloride in pyridine to give 29 (J. D. Fissekis, F. Sweet, J. Org. Chem. 1973, 38, 264).
- the 2′-benzoate 36 was tritylated in the 4′-position in yields. of greater than 90% using dimethoxytrityl chloride in the presence of Hünig's base in dichloromethane.
- the rearrangement of 4′-DMT-2′-benzoate 37 to the 4′-DMT-3′-benzoate 38 was carried out in the presence of DMAP, p-nitrophenol and Hünig's base in n-propanol/pyridine 5:2. After chromatography, 38 is obtained.
- 4′-DMT-3′-benzoate 38 was finally reacted with ClP(OAll)N(iPr) 2 in the presence of Hünig's base to give the phosphoramidite 39 (Scheme 6). This can be employed for the automated oligonucleotide synthesis without changing the synthesis protocol.
- N-phthaloyltryptamine is obtained from phthalic anhydride and tryptamine as described (Kuehne et al J. Org. Chem. 43, 13, 1978, 2733–2735). This is reduced with borane-THF to give the indoline (analogously to A. Giannis, et al., Angew. Chem. 1989, 101, 220).
- the 3-substituted indoline is first reacted with ribose to give the nucleoside triol and then with acetic anhydride to give the triacetate.
- the mixture is oxidized with 2,3-dichloro-5,6-dicyanoparaquinone and the acetates are cleaved with sodium methoxide, benzoylated selectively in the 2′-position, DM-tritylated selectively in the 4′-position, and the migration reaction is carried out to give the 3′-benzoate.
- the formation of the phosphoramidite is carried out in the customary manner. This can be employed for the automated oligbnucleotide synthesis without alteration of the synthesis protocols.
- 6-Amino-2(S)-hydroxyhexanoic acid (1) was prepared from L-lysine in a manner known from the literature by diazotization and subsequent hydrolysis (K.-I. Aketa, Chem. Pharm Bull. 1976, 24, 621).
- indole linker phosphoramidite and 244 mg of sphoramidite are weighed into a synthesizer vial and left in a high vacuum for 3 h in a desiccator over KOH together with the column packed with 28.1 mg of CDP support, loaded with A unit.
- the phosphoramidites are dissolved 1 ml (indole linker) or 2.5 ml (A phosphoramidite) of acetonitrile and a few beads of the molecular sieve are added and left closed in a desiccator over KOH.
- the support is suspended using aqueous 0.1 molar sodium diethyldithiocarbamate solution and left at RT. For 45 min. It is filtered off with suction, and washed with water, acetone, ethanol and dichloromethane.
- the support is suspended in 1.5 ml of 24% strength hydrazine hydrate solution, shaken for, 24–36 h at 4° C. and diluted to 7 ml with 0.1 molar triethylammonium hydrogencarbonate buffer (TEAB buffer). It was washed until hydrazine-free by means of a Waters Sep-Pak cartridge. It is treated with 5 ml of an 80% strength formic acid solution, and concentrated to dryness after 30 min.
- TEAB buffer triethylammonium hydrogencarbonate buffer
- a p-RNA oligomer of the sequence A 8 i.e. an octamer, is first prepared on the Eppendorf Ecosyn D 300+ as described in Example 2 and the following reagents are then exchanged: 6% strength dichloroacetic acid for 2% strength trichloroacetic acid, iodine in collidine for iodine in pyridine, benzimidazolium triflate solution for tetrazole solution.
- a DNA oligomer of the sequence GATTC is further synthesized according to known methods (M. J. Gait, Oligonucleotide Synthesis, IRL Press, Oxford, UK 1984). The deallylation, hydrazinolysis, HPL chromatography and desalting is carried out as described for the p-RNA oligomer (see above) and yields the desired conjugate.
- a p-RNA oligomer having the sequence 4′-indole linker-A 8 -2′ is prepared, purified, and iodoacetylated.
- a DNA oligomer of the sequence GATTC-thiol linker is synthesized according to known methods (M. J. Gait, Oligonucleotide Synthesis, IRL Press, Oxford, UK 1984) and purified (3′-thiol linker from Glen Research: No. 20-2933). On allowing the two fragments to stand (T. Zhu et al., Bioconjug. Chem. 1994, 5, 312) in buffered solution, the conjugate results, which is finally purified by means of HPLC.
- a p-RNA oligomer of the sequence TAGGCAAT which is provided with an amino group at the 4′-end by means of the 5′-amino modifier 5 of Eurogentec (2-(2-(4-monomethoxytrityl)aminoethoxy)ethyl 2-cyanoethyl (N,N-diisopropyl)phosphoramidite), was synthesized and worked up.
- the oligonucleotide (17.4 OD, 0.175 ⁇ mol) was taken up in 0.5 ml of basic buffer, 1.14 mg (2.5 ⁇ mol) of biotin-N-hydroxysuccinimide ester were dissolved in 114 ⁇ l of DMF (abs.) and the solution was allowed to stand at RT for 1 h.
- the resulting conjugate was purified by means of preparative HPLC and the pure product was desalted using a Sepak.
- the last DMT (dimethoxytrityl) or MMT (monomethoxytrityl) protective group was not removed from biotin or cyanine monomers.
- the detection of the last coupling with the modified phorphoramidites was carried out after the synthesis with 1% of the resin by means of a trityl cation absorption in UV (503 nm).
- the allyl ether protective groups were removed with a solution of tetrakis(triphenylphosphine)palladium (272 mg), triphenylphosphine (272 mg) and diethylammonium hydrogencarbonate in CH 2 Cl 2 (15 ml) after 5 hours at RT.
- the glass supports were then washed with CH 2 Cl 2 (30 ml), acetone (30 ml) and water (30 ml).
- the resin was rinsed with an aqueous 0.1 M sodium diethyldithiocarbamate hydrate solution. The abovementioned washing operation was carried out once more in a reverse order.
- the resin was then dried in a high vacuum for 10 minutes.
- the removal step from the glass support with simultaneous debenzoylation was carried out in 24% hydrazine hydrate solution (6 ml) at 4° C.
- the oligonucleotide “Trityl ON” was freed from the hydrazine by means of an activated (acetonitrile, 20 ml) Waters Sep-Pak Cartridge.
- the hydrazine was washed with TEAB 0.1 M (30 ml).
- the oligonucleotide was then eluted with acetonitrile/TEAB, 0.1 M (10 ml).
- the oligos were freeze-dried for storage.
- Buffer A 0.1 molar triethylammonium acetate buffer in water
- Buffer B 0.1 molar triethylammonium acetate buffer in water:acetonitrile 1:4
- a Waters Sep-Pak Cartridge RP-18 (from 15 OD 2 g of packing) was activated with 2 ⁇ 10 ml of acetonitrile and 2 ⁇ 10 ml of water, the oligo was applied and allowed to sink in, and the reaction vessel was washed with 2 ⁇ 10 ml of water, rewashed with 3 ⁇ 10 ml of water in order to remove salt and reagent, and eluted first with 5 ⁇ 1 ml of 50:1 water: acetonitrile and then with 1:1 water:acetonitrile. The product eluted in the 1:1 fractions in very good purity. The fractions were concentrated in the cold and in the dark, combined, and concentrated again.
- the yields were determined by means of UV absorption spectrometry at 260 nm.
- Buffer system Borax/HCl buffer from Riedel-de Ha ⁇ n, pH 8.0, was mixed in the ratio 1:1 with a 10 millimolar solution of EDTA disodium salt in water and adjusted to pH 6.3 using HCl. A solution was obtained by this means which contained 5 mM Na 2 EDTA.
- the standard conditions are:
- Buffer A 0.1 molar triethylammonium acetate buffer in water
- Buffer B 0.1 molar triethylammonium acetate buffer in water:acetonitrile 1:4
- Buffer system Borax/HCl buffer from Riedel-de Haen, pH 8.0, was mixed in the ratio 1:1 with a 10 millimolar solution of EDTA disodium salt in water and adjusted to pH 6.6 using HCl. A solution was obtained by this means which contains 5 mM Na 2 EDTA.
- the batch was left at room temperature in the dark until conversion was complete.
- the reaction was monitored by means of HPLC analysis. In this case, the starting material had disappeared after 70 hours.
- Buffer A 0.1 molar triethylammonium acetate buffer in water
- Buffer B 0.1 molar triethylammonium acetate buffer in water:acetonitrile 1:4
- a Waters Sep-Pak Cartridge RP-18 (from 15 OD 2 g of packing) was activated with 3 ⁇ 10 ml of acetonitrile and 3 ⁇ 10 ml of water, the oligo was applied and allowed to sink in, the reaction vessel was rewashed with 2 ⁇ 10 ml of water, and the cartridge was rewashed with 3 ⁇ 10 ml of water in order to remove salt and excess peptide, and eluted with 1:1 water: acetonitrile until no more product eluted by UV spectroscopy. The fractions were concentrated in the cold and in the dark, combined, and concentrated again.
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US10/150,402 US7153955B2 (en) | 1997-09-22 | 2002-05-16 | Pentopyranosyl nucleic acid arrays, and uses thereof |
US11/499,543 US7501506B2 (en) | 1997-09-22 | 2006-08-03 | Pentopyranosyl nucleic acid conjugates |
US12/389,789 US7777024B2 (en) | 1997-09-22 | 2009-02-20 | Process for preparing a pentopyranosyl nucleic acid conjugate |
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DE19741715A DE19741715A1 (de) | 1997-09-22 | 1997-09-22 | Pentopyranosyl-Nucleosid, seine Herstellung und Verwendung |
US09/509,039 US6506896B1 (en) | 1997-09-22 | 1998-09-21 | Use of a pentopyranosyl nucleoside for producing an electronic component, and conjugates of said pentopyranosyl nucleoside |
PCT/EP1998/005999 WO1999015541A2 (de) | 1997-09-22 | 1998-09-21 | Verwendung eines pentopyranosyl-nucleosids zur herstellung eines elektronischen bauteils sowie konjugate davon |
US10/150,402 US7153955B2 (en) | 1997-09-22 | 2002-05-16 | Pentopyranosyl nucleic acid arrays, and uses thereof |
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PCT/EP1998/005999 Continuation WO1999015541A2 (de) | 1997-09-22 | 1998-09-21 | Verwendung eines pentopyranosyl-nucleosids zur herstellung eines elektronischen bauteils sowie konjugate davon |
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US09/509,039 Expired - Lifetime US6506896B1 (en) | 1997-09-22 | 1998-09-21 | Use of a pentopyranosyl nucleoside for producing an electronic component, and conjugates of said pentopyranosyl nucleoside |
US10/150,402 Expired - Fee Related US7153955B2 (en) | 1997-09-22 | 2002-05-16 | Pentopyranosyl nucleic acid arrays, and uses thereof |
US10/644,592 Pending US20040198966A1 (en) | 1997-09-22 | 2003-08-19 | Pentopyranosylnucleoside, its preparation and use |
US10/654,274 Abandoned US20050053945A1 (en) | 1997-09-22 | 2003-09-02 | Process for the preparation of a pentopyranosyl conjugate |
US11/499,543 Expired - Fee Related US7501506B2 (en) | 1997-09-22 | 2006-08-03 | Pentopyranosyl nucleic acid conjugates |
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US09/509,058 Expired - Lifetime US6608186B1 (en) | 1997-09-22 | 1998-09-21 | Pyranosyl nucleic acid conjugates |
US09/509,039 Expired - Lifetime US6506896B1 (en) | 1997-09-22 | 1998-09-21 | Use of a pentopyranosyl nucleoside for producing an electronic component, and conjugates of said pentopyranosyl nucleoside |
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US11/499,543 Expired - Fee Related US7501506B2 (en) | 1997-09-22 | 2006-08-03 | Pentopyranosyl nucleic acid conjugates |
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