US5631348A - Protein sequence of the plant toxin gelonin - Google Patents

Protein sequence of the plant toxin gelonin Download PDF

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US5631348A
US5631348A US08/254,662 US25466294A US5631348A US 5631348 A US5631348 A US 5631348A US 25466294 A US25466294 A US 25466294A US 5631348 A US5631348 A US 5631348A
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gelonin
sequence
fragment
amino acid
protein
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Michael Rosenblum
William J. Kohr
Bharat Aggarwal
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/55—Fusion polypeptide containing a fusion with a toxin, e.g. diphteria toxin

Definitions

  • This invention relates to substantially purified gelonin, toxic fragments thereof, the DNA sequences encoding gelonin and use of the DNA for producing, by recombinant technology, gelonin, toxic fragments thereof and fusion proteins. More specifically, the invention relates to the primary amino acid sequence of gelonin, and of the DNA encoding said gelonin and the production of synthetic gelonin and toxic fragments thereof.
  • Toxic moieties thus far investigated with these delivery agents include radionuclides (Ghose, et al. (1967) Br, Med, J. 1:90-96), cytotoxic drugs commonly employed in cancer chemotherapy (Thorp and Ross (1982) Immun. Rev, 62:119-157; Deweger, et al. (1982) Immun. Rev. 62:29-45; Arnon and Sela (1982) Immun. Rev. 62:5-27; Pimm, et al. (1982) Cancer Immun. Immunotherap, 12:125-134; Rowland and Axton (1985) Cancer Immun. Immunotherap. 19:1-7) and proteins derived from bacteria and plants such as diptheria or ricin (Jansen, et al.
  • a specific molecule is designed by replacing the nonspecific B chain with an antibody or a hormone.
  • Bacterial and plant toxins such as diphtheria toxin (DT), Pseudomonas aeruginosa toxin A, abrin, ricin, mistletoe, modeccin, and Shigella toxin, are potent cytocidal agents due to their ability to disrupt a critical cellular function.
  • DT and ricin inhibit cellular protein synthesis by inactivation of elongation factor-2 and inactivation of ribosomal 60s subunits, respectively (Bacterial Toxins and Cell Membranes, Eds. Jelajaszewicz and Wadstrom (1978) Academic Press, p. 291).
  • These toxins are extremely potent because they are enzymes and act catalytically rather than stoichiometrically.
  • the molecules of these toxins are composed of an enzymatically active polypeptide chain or fragment, commonly called “A” chain or fragment, linked to one or more polypeptide chains or fragments, commonly called “B” chains or fragments, that bind the molecule to the cell surface and enable the A chain to reach its site of action, e.g., the cytosol, and carry out its disruptive function.
  • the act of gaining access to the cytosol is called variously "internalization", “intoxication”, or “translocation”.
  • These protein toxins belong to a class bearing two chains referred to as A and B chains.
  • the B chain has the ability to bind to almost all cells whereas the cytotoxic activity is exhibited by the A chain. It is believed that the A chain must be timely liberated from the B chain-frequently by reduction of a disulfide bond-in order to make the A chain functional.
  • These natural toxins are generally not selective for a given cell or tissue type because their B chains recognize and bind to receptors that are present on a variety of cells.
  • toxin molecule which is not cytotoxic to a variety of cells when administered alone has been limited.
  • Such naturally occurring single chain toxins known to date include, but are not limited to, pokeweed antiviral protein (Ramakrishnan and Houston (1984) Cancer Res. 44:201-208), saponin (Thorpe, et al. (1985) J. Natl, Cancer Inst. 75:151-159), and gelonin (Stirpe, et al (1980) J. Biol. Chem.
  • Gelonin is a single chain polypeptide isolated from seeds of a plant, Gelonium multiforum, having a molecular weight of approximately 28,000-30,000 kd.
  • Gelonin is a basic glycoprotein with an approximate isoelectric point of 8.15 and contains mannose and glucosamine residues (Falasca, et al. (1982) Biochem J, 207:505-509).
  • this protein In contrast to other plant and bacterial toxins, this protein is not toxic to cells by itself, but when delivered to cells through a carrier, it damages the 60s ribosomal subunit. In vivo and in vitro biological data suggest that gelonin is equivalent or superior to other plant toxins.
  • gelonin as a cytotoxic agent chemically attached to monoclonal antibodies or to peptide hormone cellular targeting ligands.
  • chemical modification of gelonin and cellular targeting moleties can reduce targeting efficiently and cytotoxic potential of gelonin itself.
  • natural sources of gelonin are subject to variability in harvesting and plant growth which can affect gelonin cytotoxic activity.
  • the ability to produce a synthetic gelonin toxin, chemically or utilizing recombinant technology, provides a plentiful, reproducible source of the toxin.
  • the present invention provides substantially pure gelonin having the amino acid sequence shown in FIG. 1.
  • the present invention also provides the DNA sequence for gelonin shown in FIG. 2. Utilization of the sequences of the present invention to produce substantially pure gelonin in plentiful amounts by recombinant technology provides abundant amounts of the toxin which were not heretofore available from natural sources.
  • FIG. 1 shows the amino acid sequence of gelonin.
  • FIG. 2 demonstrates the cDNA encoding for gelonin.
  • FIG. 3 demonstrates the homology of the gelonin amino acid sequence with the sequence of trichosanthin, Ricin A chain, Agglutinin precursor isolated from Castor bean and Abrin A chain.
  • FIG. 4 demonstrates the HPLC profile of CNBr fragments.
  • FIGS. 5A, 5B and 5C demonstrates the HPLC profile of (A) Lys-c, (B) Staphyloccus protease, and (C) Hydroxylamine digests of gelonin.
  • FIGS. 6A, 6B, 6C and 6D demonstrates the hydrophobicity plots of gelonin (A), trichosanthin (B), abrin (C), ricin (D), and agglutinin precursor (E).
  • substantially pure when applied to the gelonin protein of the present invention means that the polypeptide is essentially free of other plant proteins normally associated with the gelonin in its natural state and exhibiting reproducible electrophoretic or chromatographic response, elution profiles, and toxic activity.
  • substantially pure is not meant to exclude artificial or synthetic mixtures of the gelonin protein with other compounds.
  • Gelonin was purified from the seeds of the plant Gilonin multiforum by techniques known to those of skill in the art.
  • the amino acid sequence was determined utilizing a modification of the Edman degradation method.
  • Internal sequence of proteins is generally obtained by digesting or cutting up the large protein molecule into smaller pieces with a combination of enzymes and chemical cleaveges.
  • native gelonin When native gelonin was exposed to various protolytic enzyzme digestions, it was found to be incompletely cleaved. This was found to be partly due to a disulfide bond in the N-terminal part of the molecule. Breaking of this bond by reduction and alkylation with iodoacetic acid yielded a fragment that was less soluable than the native material at the pH required for enzymatic digestion.
  • Gelonin was cleaved with cyanogen bromide into 3 large peptides. Protein aliquots (0.2 mg/ml) were dissolved in 70% formic acid. A crystal of cyanogen bromide was added to the solution and the reaction allowed to proceed for at least 18 hours. The solution was then diluted with water and was applied to a small sequencing column. After sample application, a gradient of 1% to 10% n-propanol with 0.1% TFA was used to elute the protein fragmants. The elution profile is shown on FIG. 4.
  • Enzymatic digestion of the whole protein or of CNBr fragmants yeilded overlapping peptides Enzymatic digestions with Lysyl endopeptidase in 0.1% SDS 100 mM Tris pH 8.0, Staphylococcus Aureus Protease in 0.1% SDS or trypsin in 0.1% Tween 20 were carried out. Gelonin contains one cysteine residue at position 49. Reduction and carboxymethylation yeilds a protein which recovers better on reverse phase HPLC and is more susceptible to enzymatic digestion. Therefore, most of the enzymatic digestions were carried out in 0.1% SDS or 0.1% Tween.
  • This method yielded a new sequence that had not been seen before.
  • This new sequence showed the existence of an Asn-Gly combination.
  • This combination of amino acids is cleaveable by a chemical method using hydroxylamine.
  • Hydroxylamine cleavage was carried out by adding 100 ug of gelonin to freshly prepared hydroxylamine (2M) in 0.2 M Tris (pH 9.0) with 2M NaCl, mm EDTA and 10% ethanol. After incubation for 7 hours at room temperature, the entire reaction mixture was applied to a sequencing column. The colulmn was then washed with 1% TFA in water and either eluted with an acetonitrile gradient or was sequenced directly as a mixture. This chemical cleavege produced a large hydrophobic peptide that contained about a 200 amino acid sequence which connected with the Asp-Ala-Pro at residue 70. The elution profile is shown on FIG. 5C.
  • Gelonin protein comprises 258 amino acids, the sequence of which is demonstrated on FIG. 1.
  • the amino acid sequence of gelonin was compared to other known sequences available in sequence data banks (Genbank, PIR, EMBL) to determine whether gelonin has any areas of homology with other proteins. Comparison of the gelonin amino acid sequence with other proteins having known amino acid sequences, demonstrated that the gelonin sequence is unique. Homology of certain portions of the gelonin sequence to portions of other proteins was detected.
  • gelonin demonstrates a 36.0% homology with alphatrichosanthin from Trichosanthin kirilowi, 33.8% homology with Abrin A chain from Indian Licquorice, 35.2% homology with agglutinin precursor from Castor bean, 33.7% homology with Ricin D, A chain from Castor bean and 27.3% homology with antiviral protein (MAP) from Mirabills jalapa.
  • MAP antiviral protein
  • Hydrophobicity plots shown on FIG. 6A-6E demonstrate a similarity to hydrophobic regions of trichosanthin, Ricin and to other ribosomal inhibiting proteins.
  • a plot of the hydropathy of the gelonin structure shows a hydrophobic region in residues 35-80 and 150-180. These are areas in which substantial folding of the molecule probably occurs. This similar hydrophobic pattern is also observed for other toxins (see FIGS. 6A-6E) and may suggest that the active enzymatic center may be contained within these folded regions. Therefore, the active enzymatic site may not be found in a linear region of the molecule and these structures may need to be adequately folded to attain the proper enzymatic center.
  • recombinant gelonin can be produced. Mutations can be specifically introduced into the molecule in order to provide recombinant gelonin lacking carbohydrate groups which can misdirect gelonin-antibody conjugates.
  • Recombinant gelonin molecules can be produced by site directed mutagenesis to have greater toxic activity than the native molecule, to be more effectively internalized once bound to the cell surface by a carrier such as a monoclonal antibody or a targeting ligand such as IL-2, EGF, IFN, etc., to resist lysosomal degradation and thus be more stable and longer acting as a toxic moiety.
  • Recombinant gelonin molecules can also be engineered as fusion products to contain other functional modalities to kill cells such as an enzymatic activity, TNF, IFN activity, a second toxic activity, such as diptheria toxin action (wherein said second activity was through a different biological pathway than gelonin), thus creating a "supertoxin” or a toxin with multifunctional actions.
  • Fusion proteins can be engineered with gelonin to carry drugs such as chemotherapeutic agents or isotopes for radioimaging or radiotherapy.
  • Gelonin peptides may have application as abortofacient agents, immuno suppressive agents, anticancer agents and as antiviral agents (such as an anti-HIV agent).
  • Gelonin was isolated from the seeds of the plant Gelonim multiforum essentially according to the procedure as described (Stirpe, et al. (1980) J. Biol, Chem 255 6947-6953). Briefly, gelonin was extracted from the seeds by homogenization in buffered saline solution (pH 7.4). The supernatant was concentrated after dialysis against 5 mM sodium phosphate (pH 6.5) and the gelonin further purified by ion exchange chromatography as described below. The purity of the gelonin toxin was assessed by high pressure liquid chromatography (HPLC) and sodium dodecylsulphate-polyacylamide gel electrophoreseis (SDS-Page). Gelonin toxin migrated as a single band with an approximate molecular weight of 29-30,000 daltons.
  • HPLC high pressure liquid chromatography
  • SDS-Page sodium dodecylsulphate-polyacylamide gel electrophoreseis
  • Gelonin toxin activity was measured as described in Example 2 by protein synthesis inhibition in a cell-free system.
  • Material which bound to the ion exchange resin was eluted with 400 ml of 0 to 0.3 M linear NaCl gradient at a rate of 25 ml hour at 4° C. Five ml fractions were collected. The fractions were monitored at 280 nm in a spectrophotometer. The gelonin eluted in about fractions 55-70 and was the last major elution peak. These fractions were pooled, dialyzed against 0.1 M NaCl in 0.1 M Na 2 HPO 4 buffer (pH 7.4). The sample was then applied to a Cibacron blue sepharose column (24 ⁇ 2 cm) previously equilibrated with 0.1 M Na 2 HPO 4 /0.1 M NaCl buffer.
  • the column was washed with 3 column volumes of buffer and eluted with a 400 ml linear salt gradient (from 0.1 M NaCl to 2 M NaCl). Elution of the bound material was monitored by Lowry assay of the column fractions. The fractions containing the single protein peak were pooled and dialyzed overnight at 4° C. against PBS. Gelonin toxin was purified to greater than 97% purity as estimated from silver stained PAGE. The purity and the molecular weight of each preparation was checked on high pressure liquid chromotography using a TSK 3000 gel permeation column with 50 mM sodium phosphate buffer, pH 7.4 and 15% sodium dodecylsulphate-polyacrylamide gel electrophoresis (SDS-page). Gelonin migrated as a single band with an approximate molecular weight of 29-30,000 daltons.
  • the gelonin activity was monitored in a cell-free protein synthesis inhibition assay.
  • the cell-free protein synthesis inhibition assay was performed by sequentially adding to 50 ul rabbit reticulocyte lysate, thawed immediately before use, mixing after each addition, the following components: 0.5 ml of 0.2 M Tris HCl (pH 7.8), 8.9 ml of ethylene glycol, and 0.25 ml of 1 M HCl).
  • SAEM salt-amino acid-energy mixture
  • 14 C-leucine incorporation was monitored in an aliquot of the mixture by precipitating synthesized protein on glass fiber filters, washing in 10% TCA and acetone, and monitoring the radioactivity in a Beta-counter using Aquasol scintillation fluid. Utilizing this assay, purified gelonin had a specific activity of 4 ⁇ 10 9 U/mg protein. A unit of gelonin activity is the amount of gelonin protein which causes 50% inhibition of incorporation of [ 14 C] leucine into protein in the cell free assay.
  • the gelonin amino acid sequence was determined by the Edman degradation method using an automated amino acid sequencer as described in European Patent Application No. EP-257735. Large peptides and unfragmented protein were applied to the reverse phase portion of the sequence reaction chamber. Unwanted buffer components were washed off with excess water. The protein or peptide sample was then sequenced by Edman chemistry and the extracted ATZ amino acid derivatives were converted to the PTH form by 25% TFA in H 2 O at 65° C. PTH samples were identified by reverse phase analytical separation on a Np 1090 column.
  • the protein was digested with various proteolytic and chemical agents and then the peptides were purified by high performances liquid chromatography.
  • Gelonin was found quite resistant to the exposure of trypsin (cleaves after arginine and lysine residues) and acetyl trypsin (cleaves only after lysine residue). The protein was found resistant to as much as 5% (w/w) of the enzyme.
  • the resistance of gelonin to the proteolytic enzyme trypsin is not due to a lack of trypsin cleavage sites, since gelonin contains 21 lysine and 12 arginine residues.
  • Peak 2 was sequenced directly and gave a sequence starting with a Val (V) that ran for 47 cy and was not interruptable after the ala at cy 47.
  • Peak 3 was sequenced and gave the same sequence as peak 2.
  • SDS gels of peaks 2 and 3 as well as Lysc digestion of peaks 2 and 3 showed that peak 3 contained the C-terminal CNBr peptide as well.
  • Subsequent trypsin digestion of gelonin produced a peptide that connected these two CNBr peptide sequences. This trypsin peptide when sequenced gave the sequence TSGANGMFSEAVELER.
  • Peak 4 and 5 both gave the N-terminal sequence GLDT . . . . This was used for some digestion by Lysc, 1/8, to give peptides from its C-terminal end.
  • Samples of whole protein or CNBr fragments were digested with Lysyl endopeptidase (Wako Chemical Dallas, Tex.) in 0.1% SDS 100 mmTris pH 8.0 or Staphylococcus Aureus Protease (Pierce) in.1% SDS or Trypsin (Sigma) in 0.1% Tween 20. Digestion mixtures were separated by HPLC and collected peptides were sequenced on the prototype sequence use gas-phase Edman sequencing methods.
  • FIG. 1 shows the amino acid sequence of gelonin.
  • Gelonin contains a total of approximately 258 amino acid residues.
  • the DNA sequence was deduced from this amino acid sequence.
  • the degenerate DNA sequence is shown on FIG. 2.
  • fragments and derivatives of either the gelonin amino acid sequence or the DNA sequence coding for gelonin may inhibit cellular protein synthesis but not bind to a cell surface receptor.

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US5837491A (en) * 1991-11-04 1998-11-17 Xoma Corporation Polynucleotides encoding gelonin sequences
US6146850A (en) * 1991-11-04 2000-11-14 Xoma Corporation Proteins encoding gelonin sequences
US6146631A (en) * 1991-11-04 2000-11-14 Xoma Corporation Immunotoxins comprising ribosome-inactivating proteins
USRE37462E1 (en) 1990-08-14 2001-12-04 The Research Development Foundation Protein sequence of the plant toxin gelonin
US20030086919A1 (en) * 2001-07-17 2003-05-08 Rosenblum Michael G. Therapeutic agents comprising pro-apoptotic proteins
US6599505B1 (en) * 1992-04-10 2003-07-29 Research Development Foundation Immunotoxins directed against CD33 related surface antigens
US20030176331A1 (en) * 2001-02-12 2003-09-18 Rosenblum Michael G. Modified proteins, designer toxins, and methods of making thereof
US20040013691A1 (en) * 2002-06-12 2004-01-22 Rosenblum Michael G. Immunotoxin as a therapeutic agent and uses thereof
US20050214307A1 (en) * 1990-04-19 2005-09-29 Research Development Foundation Antibody conjugates for treatment of neoplastic disease
US20060171919A1 (en) * 2005-02-01 2006-08-03 Research Development Foundation Targeted polypeptides
WO2014127211A1 (en) 2013-02-15 2014-08-21 Research Development Foundation Deimmunized gelonin molecules and therapies

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RU2142015C1 (ru) * 1991-09-06 1999-11-27 Рисерч Дивелопмент Фаундейшн Фрагмент днк, кодирующий белок гелонина, и способ его получения, вектор, обеспечивающий экспрессию белка гелонина, рекомбинантный штамм e.coli - продуцент белка гелонина
US5684129A (en) * 1992-07-07 1997-11-04 Fish; Eleanor N. Interferon receptor binding peptides

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