US20080299092A1 - Cosmetic preparation and method for preparing the same - Google Patents

Cosmetic preparation and method for preparing the same Download PDF

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US20080299092A1
US20080299092A1 US12/148,241 US14824108A US2008299092A1 US 20080299092 A1 US20080299092 A1 US 20080299092A1 US 14824108 A US14824108 A US 14824108A US 2008299092 A1 US2008299092 A1 US 2008299092A1
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dedifferentiated
stem cells
cosmetic preparation
set forth
cells
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Peter Blum
Cornelia Schurch
Daniel Schmid
Fred Zulli
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Mibelle AG
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Mibelle AG
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Assigned to MIBELLE AG reassignment MIBELLE AG ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: BLUM, PETER, SCHMID, DANIEL, SCHURCH, CORNELIA, ZULLI, FRED
Publication of US20080299092A1 publication Critical patent/US20080299092A1/en
Priority to US13/443,995 priority Critical patent/US8580320B2/en
Priority to US14/075,777 priority patent/US9155916B2/en
Abandoned legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/14—Liposomes; Vesicles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/10—Washing or bathing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q5/00—Preparations for care of the hair
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q7/00—Preparations for affecting hair growth
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
    • A61K9/113—Multiple emulsions, e.g. oil-in-water-in-oil
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes

Definitions

  • the present invention relates to the use of dedifferentiated plant cells in cosmetic preparations for protecting of stem cells against intrinsic and extrinsic stress factors, in particular for promoting proliferation of stem cells and for protecting them against apoptosis.
  • the invention relates to the use of dedifferentiated plant cells from fruits of Malus domestica (Apple) cultivar Uttwiler Spaetlauber.
  • the invention relates to a method for cultivating of dedifferentiated plant cells, as well as to the preparation of extracts of plant cell cultures which are suitable for such applications.
  • SC Stem cells
  • SC may be divided into two groups, i.e. embryonal and adult (EC).
  • Embryonal stem cells play a key role in the first development phase of an organism. They are able to endlessly cleave and to develop every necessary type of tissue. Thus, they are able to form from a single cell a whole body, either the plant or the animal form which they originate. Due to this ability they are also called pluripotent cells. Unfortunately, for human beings this ability is restricted to the embryonal phase. In later phases of life of a subject EC are no longer present.
  • the second type of stem cells are adult stem cells. So far, these cells could be identified in many full-grown tissues and organs, such as bone marrow, pancreas, spine, brain, central nervous system, peripheral blood, dental pulp, blood vessel, skeletal muscles, cornea, retina, liver, cord blood, heart, epithelium of the intestinal tract, and dermis.
  • the skin of mammals is a multilamina system which is continuously revolving.
  • the part which is constantly in contact with the outside world is called epidermis.
  • the major task of this specialized Tissue is to protect the body against dehydration, lesions and infections. It is composed four different laminas which are all formed by a single cell type, the so-called cerationocytes. Whereas this cell type is not much differentiated, is nevertheless has its origin in specialized skin stem cells. They are located in the lowermost lamina of the epidermis, the basal lamina.
  • Vegetable extracts and the use of parts of plants, such as leaves, fruits, flowers, stems, bark, inflorescences und roots for cosmetic and medical Applications are known since ancient times. Products derived therefrom may be e.g. essential oils, fibers, starch, flavors, coloring matters, antibiotics, proteins, phenols, acids or fats.
  • the use of plants or plant extracts in cosmetics is rampant. There are a great many of different uses, such as humidification, brighteners, tanning lotions, make-ups, sun filters, scavengers, antioxidants, immunity stimulation, detergents, preserving agents or thickening agents.
  • useful plants and plant component comprises e.g. algae, succulents, berries, carnivorous plants, herbs, cereals and trees.
  • Usual well known examples of plants, however not limited to them, are: Spirulina algae, aloe vera, calendula, ginkgo, ginseng, iris, valerian, sage, lavender, thyme, peppermint, Saint-John's-wort, citrons, peach, guava, avocado, wheat, and oat.
  • the utilization of methods of plant cell culture techniques may help to solve such problems.
  • Said utilization comprises techniques which allow, when observing certain known process steps, to obtain uniform dedifferentiated cells, showing the following advantages as compared with cultivated whole plants:
  • dedifferentiated plant cells utilizes the biological fact, that every plant cell has the ability to build up the whole plant which the cell stems from. This ability is called totipotency and is comparable with the pluripotency of animal ES. Therefore, it may be accepted that dedifferentiated plant cells do have a positive influence on protection and activation of skin stem cells.
  • the main ingredients of apples are various sugars, vitamins, acids, oils, waxes and polyphenols. Recently published studies could prove that especially the overall polyphenols in apple extracts or apple juices rich in polythenols can be useful in preventing and combating colon cancer (Eberhart et al., 2000, Antioxidant Activity of Fresh Apples, Nature, 405, 903 to 904; Liu et al., 2003, Antiproliferative Activity of Apples is not due to Phenolic-induced Hydrogen Peroxide Formation, J. Agric. Food Chem., 51, 1718 to 1723; Kem et al., 2005, Inhibitors of the Epidermal Growth Factor Receptor in Apple Juice Extract, Mol. Nutr.
  • apple show a large antioxidative activity and can increase the antioxidative capacity in blood (Rezk et al., 2002, The Antioxidant Activity of Phloretin: The Disclosure of a new Antioxidant Pharmacophore in Flavonoids, Biochem. Biophys. Res. Commun., 295, 9 to 13; Lee et al., 2003, Major Phenolics in Apple and their Contribution to the Total Antioxidant Capacity, J. Agric. Food Chem., 51, 6516 to 6520; Vrohovsek et al., 2004, Quantitation of Polyphenols in Different Apple Varieties, J. Agric.
  • Dedifferentiated plant cells have a complex matrix of constituents of salts, acids, polyphenols, sugars, fats, proteins and other components.
  • constituents of salts, acids, polyphenols, sugars, fats, proteins and other components In addition to known components there is an unknown fraction of components which possibly is very valuable for cosmetical applications. It is known that raw plat extracts often show a better effect than identified and isolated individual components. Therefore, it is reasonable to use the entire cell lysate for application.
  • liposomes Since transport of materials through the skin barrier is very limited, the technique of producing liposomes for many cosmetic applications was developed (e.g. KR20050091162, KR920005639B, GB2415375, WO2004067012, EP1498420, US2002160064, AU2388099). Application of this technique allows a better penetration of substances into the lower skin laminas. Also, a further advantage of liposomes is the encapsulation of fat-soluble ingredients in the membrane and thus their dispersion in aqueous phases.
  • Main steps of their production comprise dissolving a phospholipid mixture in a suitable solvent (e.g. glycerol or alcohol), intermixing the dissolved lipids with an aqueous phase, applying energy (e.g. by stirring, shaking, pressure or heat) for forming the liposomes.
  • energy e.g. by stirring, shaking, pressure or heat
  • the form of energy can by pressure.
  • Formation of liposomes by means of high pressure homogenization is a known technique. Examples for pharmaceutic or cosmetic preparations may be found e.g. in WO9949716, NZ502840 or EP0782847.
  • solubilizing cells and obtaining their lysate e.g. DE19918619. Therefore, it is possible to solubilize plant cells of suspension cultures and at the same time to extract the oil- and water-soluble agents into empty liposomes. Thereby, stability of the agents and their transportation into the skin can be improved.
  • EP 1,064,932 A1 proposes the use of extracts of dedifferentiated plant cells in deodorants. Other uses are not disclosed.
  • WO 03/077881 A discloses the use of lysates of metabolites of dedifferentiated cells of vine, which were obtained by means of a complicated method, for the preparation of cosmetics. This technique calls for the use of lyophilizates. A use for stimulation and protection of skin stem cells is not envisaged. Furthermore, other species of plants are not disclosed.
  • the main object of the present invention is to create a cosmetic preparation which protects stem cells against intrinsic and extrinsic stress factors, in particular promotes the proliferation of stem cells and protects them against apoptosis.
  • Another object of the present invention is to provide a method for preparing an extract suitable for use in said cosmetic preparation.
  • the abovementioned object is achieved by using an extract of a suspension of dedifferentiated plant cells, preferably of the family of Rosaceae (Rose family), particularly of the subfamily Maloidae (Pome fruit), and more in particular of Malus domestica cultivar Uttwiler Spaetlauber, which is an old and rare kind of apple.
  • Rosaceae Rosaceae
  • Maloidae Pome fruit
  • Malus domestica cultivar Uttwiler Spaetlauber which is an old and rare kind of apple.
  • the method of preparing suitable extracts comprises the following main steps:
  • step (c) the following procedure is followed:
  • FIG. 1 shows the increase in the cell count of umbilical cord stem cells in dependence of different concentrations of a liposomal extract originating from dedifferentiated cells of apples of the cultivar Uttwiler Spaetlauber.
  • the extract was centrifuged and sterilized by filtration.
  • FIG. 2 shows the increase in proliferation capability in a MTS-assay of umbilical cord stem cells in dependence of different concentrations of a liposomal extract originating from dedifferentiated cells of apples of the cultivar Uttwiler Spaetlauber.
  • the extract was centrifuged and sterilized by filtration.
  • FIG. 3 shows microscopical pictures of umbilical cord stem cells.
  • the left photograph shows cells cultivated in a medium without extract, the right photograph cells cultivated together with 0.1 percent of a liposomal extract originating from dedifferentiated cells of apples of the cultivar Uttwiler Spaetlauber.
  • the extract was centrifuged and sterilized by filtration.
  • FIG. 4 shows the proliferation capability of umbilical cord stem cells of a control preparation and of a liposomal extract of different concentrations originating from dedifferentiated cells of apples of the cultivar Uttwiler Spaetlauber, 48 hours after UV irradiation.
  • the extract was centrifuged and sterilized by filtration.
  • FIG. 5 shows the temporal influence of a liposomal extract according to Example 10 on the length of hair follicles.
  • FIG. 6 shows the effect of a preparation in accordance with the present invention as anti-wrinkle cream in Test 1 described hereafter.
  • FIG. 7 shows the effect of a preparation in accordance with the present invention on stressed skin in Test 2 described hereafter.
  • OD is the abbreviation of “optical density”.
  • the obtained suspension culture is cultivated further over several continuous steps from small laboratory flasks (Erlenmeyer flask usually having 200 ml content) to production scale of 50 to 100 liters.
  • small laboratory flasks Erlenmeyer flask usually having 200 ml content
  • production scale 50 to 100 liters.
  • 5 to 10 percent, preferably 10 percent, of the next culture volume of a fully grown cell suspension is used as inoculum.
  • the scale-up may be done in steps of e.g. 0.1/1/10/100 liter.
  • Cultivation volumes exceeding 1 liter necessitate the use of special bioreactors instead of culture flasks used before.
  • Many different systems are available on the market. Execution of cultivation is done, but is not limited thereto, in agitation reactors, bubble columns, loop reactors or newly developed one-way systems suitable for plant cell cultivation. Fir all these cultures the influence of shearing stress which can endamage the cultures. Thus, the most important parameter for selecting a suitable reactor system usually is the manner how the culture is homogenized.
  • control of the culture is very important. In comparison to cultures of yeast or bacteria, measurement of the biomass is difficult, and the growth of biomass has to be measured by means of indirect parameters, such as e.g. consumption of carbon, dropping of conductivity or the pH value or the increase of optical density. Once such a control is established, the end point or the harvest moment, respectively, can be fixed.
  • the cells are solubilized by means of liposomes.
  • the main component of this method is the use of high pressure homogenization of the whole cell broth together with a liposome preparation.
  • the great advantage of this method is its simple and low-cost application.
  • the method comprises the following steps:
  • preservative agents of natural or synthetic origin allowed for cosmetics such as e.g. phenoxyethanol, benzoic acid, propionic acid, alcohol or silver chloride, can be used as preservative agents.
  • antioxidants such as e.g. ascorbic acid or tocopherol, may be added.
  • the described method allows the addition of still further substances useful in the preparation or cosmetic product. Once all compounds are added, the mixture has to be stirred in order to dissolve the preservative agents and other components. This may be done e.g. by means of a paddle mixer, a homogenization rod or by pumping through static mixing elements.
  • Suitable high pressure homogenizators are commercially available on the market.
  • the principle of the reaction chamber has to be selected from different possibilities and has to be previously tested.
  • the number of passages through the reaction chamber necessary for a disintegration of all cell membranes or reaching a desired homogeneity of the extract has to be tested as well.
  • the extract obtained in this manner can directly be incorporated into cosmetic preparations, such as e.g. creams, soaps, lotions, gels or hair seras. If the extract is to be used as semi-finished good a supplemental thickening is possible. All thickening agents of natural or synthetic origin allowed for cosmetics can be used as thickening agents.
  • the ph-value was adjusted to 5.6 with sodium hydroxide solution.
  • Agar was added in a concentration of 0.8 percent as gelling agent. All ingredients were mixed together and sterilized at 121° centigrade for 15 minutes.
  • the induction of the primary callus was carried out in the dark at 25° centigrade.
  • the formed calluses were harvested after two to three weeks and further incubated on the same medium. Several sub-cultivations were carried out until the callus was fully dedifferentiated.
  • Dedifferentiated cell clumps growing on said solid medium were taken, homogenized and placed into the same medium without gelling agent. A finely dispersed suspension was obtained which could be use for larger cultivation systems. The suspensions were grown in the dark at 25 centigrade and a shaking velocity of about 100 rpm.
  • the whole cell broth was mixed with a dispersion containing empty liposomes of a size of about 50 nanometer.
  • the mixture was then four times high pressure homogenized at a pressure of about 1200 bar (1.2*10 8 N m ⁇ 2 ) resulting in a finely dispersed extract.
  • Oily phase 1 Alkyl benzoates 10% Dimeticone 3% Archidyl glycosides 3% Myristyl glycoside 2% Oily phase 2: Polyacrylamides 1% Aqueous phase: Demineralized water 71% Glycerol 5% Phenoxyethanol 1%
  • Oily phase 1 and the aqueous phase were heated at 80 centigrade and blended. The mixture was chilled to 60 centigrade. Then oily phase 2 was added, and the mixture was blended. The mixture was chilled to 30 centigrade. 4 percent of the extract described in Example 4 was added and the mixture was blended again.
  • Liquid balm for the scalp The percentage refers to the total quantity (weight/weight). Ethanol 0.5% Urea 5% Propylene glycol 0.5% Carbomer 0.4% Bisabololene 0.1% PEG-60 0.6% D-Panthenol 75% 0.5% Sodium hydroxide 30% 0.4% Plant cell extract of Example 4 1% Water filling up to 100%
  • Phase Ingredient Amount Aqueous phase 1 (W1) Water filling up to 100% Citric acid 0.6% Sodium benzoate 0.5% Aqueous phase 2 (W2) D-Panthenol 75% 0.7% Oily phase 1 (O1) Cetearyl alcohol 4.5% Dicocoylethyl hydroxyethlmonium methosulfate 3% Distearoylethyl hydroxetylmonium methosulfate 1.5% Dicapryryl ether 1% Gycerol stearate 1% Oily phase 2 (O2) Amino dimethicone 0.3% Plant extract (A) Pant cell extract of Example 4 2%
  • Aqueous phase 1 is mixed and heated to 75 centigrade. Shortly before mixing with oily phase 2 aqueous phase 2 (panthenol) is added. Oily phase 1 is heated to 75 centigrade, and shortly before mixing oily phase 2 (aminodimethicone) is added. The combined aqueous and oily phases are mixed and homogenized. The mixture is chilled to 30 centigrade, and phase A (plant extract) is added.
  • Phase Ingredient Amount Aqueous phase 1 (W1) Water filling up to 100% Citric acid 0.6% Glycerol 5% Butylen glycol 5% Galacto arabinane 0.3% Parabens in phenoxyethanol 0.8%
  • Oily phase 1 (O1) Polyglyceryl-3-methylglucose distearate 2.5% Hydrogenated polyisobutene 3% Vegetable oil 4% Dicapryryl ether 3% Behenyl alcohol 2% Dimethicone 0.5% Oily phase 2 (O2) Maize phosphates 1% Dimethicone 0.5% Plant extract (A) Plant cell extract of Example 4 2%
  • Aqueous phase is mixed and heated to 80 centigrade.
  • Oily phase 1 is heated to 80 centigrade, and oily phase 2 is added.
  • the combined aqueous and oily phases are mixed and homogenized.
  • the mixture is chilled to 30 centigrade, and phase A (plant extract) is added, and the blend is mixed again.
  • the test was carried out on stem cells originating from the umbilical cord.
  • the cells were grown in a complex medium containing 10 percent of fetal calf serum. The supernatant with out cell debris was used for the test. Previous to the test, the extract was sterilized by filtration.
  • the epithelium of the hair root bulges into a suprabasal bulge which is the niche of the ceratinozyte stem cells. They consist of clonal subpopulations which regenerate skin and hair follicles. Thus, isolated hair follicles are a suitable model for analyzing the life expectancy of stem cells.
  • Hair follicles were isolated from skin material originating from an esthetic surgery. Then they were placed in a nutrient solution where they bed on and started growing. In this manner, hair follicles could kept alive for about 14 days. Thereafter, the cells begin to die off, and the newly formed hair begins to shrink a control assay of 12 follicles was incubated in the nutrient solution only, Whereas a second series was incubated in a nutrient solution containing 0.2 percent of a liposomal extract of dedifferentiated cells of apples of the cultivar Uttwiler Spaetlauber. On the 16th, 18th and 20th day the length of the hair follicles was measured.
  • the ex vivo test showed that, as excepted, the follicles of the control assay had lost about 6 percent of its length already on the 16th day. A similar shrinking could be asserted on the 18th day. Then, on the 20th day a considerable dying of 52 percent was measurable. The follicles treated with the extract remained longer in the growth phase. On the 16th day, an increase in length of 8 percent could still be measured. Not until the 18th day a slight shrinking arised. The dying on the 20th day was clearly lesser than in the control assay.
  • Example 10 shows that a liposomal extract of dedifferentiated cells of apples of the cultivar Uttwiler Spaetlauber is able to prolong the expectancy of life of cerationocyte stem cells.
  • the tumor suppressor gene p53 In normal skin the tumor suppressor gene p53 is upregulated by several types of stress, e.g. DNA damage (induced by UV radiation, IR radiation, or chemical agents, such as hydrogen peroxide), oxidative stress, or osmotic shock.
  • DNA damage induced by UV radiation, IR radiation, or chemical agents, such as hydrogen peroxide
  • oxidative stress oxidative stress
  • osmotic shock oxidative stress
  • the protein p53 plays an important role in the cell cycle as transcription regulator. In old skin this gene is no more upregulated but rather down regulated by stress.
  • Fibroblasts were stressed for 2 hours with culture medium containing 600 ⁇ mole of H 2 O 2 .
  • the cells were incubated for 72 hours with a medium containing, or not containing (control), 2% of PhytoCellTecTM Malus Domestica .
  • mRNA was extracted and transcribed into 33 P-labeled cDNA via reverse-transcription.
  • These labeled cDNA targets were hybridized to an “old skin” specific minichip. This minichip contained about 150 genes specific for skin aging. The content of labeled genes on the minichip was measured.
  • H 2 O 2 -stressed fibroblasts p53 was downregulated.
  • H 2 O 2 -stressed cells treated with 2% PhytoCellTecTM Malus Domestica showed an upregulation of p53.

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US13/443,995 US8580320B2 (en) 2007-04-27 2012-04-11 Cosmetic preparation and method for preparing the same
US14/075,777 US9155916B2 (en) 2007-04-27 2013-11-08 Cosmetic preparation and method for preparing the same

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CH00701/07A CH715456B1 (de) 2007-04-27 2007-04-27 Kosmetisches Produkt zur topischen Anwendung für den Schutz und die Erneuerung von Hautstammzellen, welches sich von dedifferenzierten Pflanzenzellen ableitet.
CH00701/07 2007-04-27

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Cited By (16)

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CN104711215A (zh) * 2015-04-09 2015-06-17 广州赛莱拉干细胞科技股份有限公司 一种苹果干细胞的培养方法及培养的苹果干细胞
EP3566751A1 (fr) 2012-02-21 2019-11-13 Lumene Oy Compositions cosmétiques contenant une préparation de culture cellulaire de mûres arctiques
CN114588100A (zh) * 2020-12-04 2022-06-07 韩国百鸥思特公司 大量含有愈伤组织代谢产物的愈伤组织溶解产物及其制备方法
CN114828820A (zh) * 2019-12-19 2022-07-29 莱雅公司 非诱发去分化薰衣草植物细胞、其提取物及其美容用途

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US20100173024A1 (en) * 2008-12-01 2010-07-08 LifeSpan Extension, LLC Methods and compositions for altering health, wellbeing, and lifespan
WO2010067212A2 (fr) * 2008-12-12 2010-06-17 Labo Cosprophar Ag Complexe de cellules souches végétales actives et composition cosmétique
WO2010067212A3 (fr) * 2008-12-12 2011-04-07 Labo Cosprophar Ag Complexe de cellules souches végétales actives et composition cosmétique
JP2011026265A (ja) * 2009-07-28 2011-02-10 Hokkaido Mitsui Chemicals Inc トリテルペン含有抽出物、フラボノイド含有抽出物の製造方法
WO2011019668A1 (fr) * 2009-08-09 2011-02-17 Meltology, Llc Formulation cosméceutique topique et procédés de préparation et d’utilisation afférents
US20110159121A1 (en) * 2009-12-24 2011-06-30 LifeSpan Extension, LLC Methods and compositions for identifying, producing and using plant-derived products for modulating cell function and aging
WO2011079212A3 (fr) * 2009-12-24 2011-11-03 LifeSpan Extension, LLC Procédés et compositions destinés à identifier, produire et utiliser des produits dérivés de plantes pour modulation de la fonction cellulaire et du vieillissement
WO2011079212A2 (fr) * 2009-12-24 2011-06-30 LifeSpan Extension, LLC Procédés et compositions destinés à identifier, produire et utiliser des produits dérivés de plantes pour modulation de la fonction cellulaire et du vieillissement
CN102477411B (zh) * 2010-11-30 2016-05-18 株式会社爱茉莉太平洋 含有苹果干细胞提取物的脂肪来源干细胞的干细胞性增进用组合物
CN102477411A (zh) * 2010-11-30 2012-05-30 株式会社爱茉莉太平洋 含有苹果干细胞提取物的脂肪来源干细胞的干细胞性增进用组合物
CN102477410A (zh) * 2010-11-30 2012-05-30 株式会社爱茉莉太平洋 含有双歧杆菌提取物的脂肪来源干细胞的干细胞性增进用以及皮肤细胞增殖用组合物
CN102477410B (zh) * 2010-11-30 2016-08-10 株式会社爱茉莉太平洋 含有双歧杆菌提取物的脂肪来源干细胞的干细胞性增进用以及皮肤细胞增殖用组合物
JP2017200922A (ja) * 2011-03-27 2017-11-09 アーチ ケミカルズ、インコーポレイテッド 幼弱細胞と関連するｄｎａメチル化パターンの採用を細胞にもたらす後成的ｄｎａメチル化の調節
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JP2014512348A (ja) * 2011-03-27 2014-05-22 アーチ ケミカルズ、インコーポレイテッド 幼弱細胞と関連するｄｎａメチル化パターンの採用を細胞にもたらす後成的ｄｎａメチル化の調節
WO2012171106A1 (fr) * 2011-06-13 2012-12-20 Levy Phillip Compositions cosmétiques pour la peau comprenant un extrait de malus domestica et un extrait de bourgeon d'argania spinosa pour améliorer l'apparence de la peau
US20150147360A1 (en) * 2011-06-13 2015-05-28 Phillip Levy Skin cosmetic compositions comprising malus domestica extract and argania spinosa sprout extract for improving skin appearance
US10149816B2 (en) * 2011-06-13 2018-12-11 Phillip Levy Skin cosmetic compositions comprising Malus domestica extract and Argania spinosa sprout extract for improving skin appearance
JP2015505312A (ja) * 2012-01-05 2015-02-19 ロレアルＬ′Ｏｒｅａｌ 脱分化植物細胞の化粧的使用
US20140356310A1 (en) * 2012-01-05 2014-12-04 L'oreal Cosmetic use of dedifferentiated plant cells
US10463603B2 (en) * 2012-01-05 2019-11-05 L'oreal Cosmetic use of dedifferentiated plant cells
WO2013120620A2 (fr) 2012-02-16 2013-08-22 Svr Recherche Combinaison de n-cocoyl alanine et d'un extrait de cellules dedifferenciees de malus domestica
EP3566751A1 (fr) 2012-02-21 2019-11-13 Lumene Oy Compositions cosmétiques contenant une préparation de culture cellulaire de mûres arctiques
CN102670444A (zh) * 2012-05-23 2012-09-19 成都鹏翔生物科技有限公司 一种肌底液及其制备方法
CN104711215A (zh) * 2015-04-09 2015-06-17 广州赛莱拉干细胞科技股份有限公司 一种苹果干细胞的培养方法及培养的苹果干细胞
CN114828820A (zh) * 2019-12-19 2022-07-29 莱雅公司 非诱发去分化薰衣草植物细胞、其提取物及其美容用途
CN114588100A (zh) * 2020-12-04 2022-06-07 韩国百鸥思特公司 大量含有愈伤组织代谢产物的愈伤组织溶解产物及其制备方法

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CH715456B1 (de) 2020-04-30
EP1985280B1 (fr) 2016-07-13
KR20080096411A (ko) 2008-10-30
KR101470632B1 (ko) 2014-12-09
EP1985280A3 (fr) 2009-11-11
US9155916B2 (en) 2015-10-13
US8580320B2 (en) 2013-11-12
US20120195948A1 (en) 2012-08-02
US20140072619A1 (en) 2014-03-13

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Owner name: MIBELLE AG, SWITZERLAND

Free format text: ASSIGNMENT OF ASSIGNORS INTEREST;ASSIGNORS:BLUM, PETER;SCHURCH, CORNELIA;SCHMID, DANIEL;AND OTHERS;REEL/FRAME:020869/0865

Effective date: 20080402

STCB Information on status: application discontinuation

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