US20070148741A1 - Mashing process - Google Patents

Mashing process Download PDF

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Publication number
US20070148741A1
US20070148741A1 US10/583,676 US58367604A US2007148741A1 US 20070148741 A1 US20070148741 A1 US 20070148741A1 US 58367604 A US58367604 A US 58367604A US 2007148741 A1 US2007148741 A1 US 2007148741A1
Authority
US
United States
Prior art keywords
xylanase
endoglucanase
seq
beta
enzyme
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
US10/583,676
Other languages
English (en)
Inventor
Rikke Festersen
Anders Nielsen
Christel Joergensen
Lars Christensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes AS
Original Assignee
Novozymes AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34684454&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=US20070148741(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Novozymes AS filed Critical Novozymes AS
Assigned to NOVOZYMES A/S reassignment NOVOZYMES A/S ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: CHRISTENSEN, LARS LEHMANN HYLLING, VIKSOE-NIELSEN, ANDERS, JOERGENSEN, CHRISTEL THEA, FESTERSEN, RIKKE MONICA
Publication of US20070148741A1 publication Critical patent/US20070148741A1/en
Priority to US12/493,587 priority Critical patent/US8679791B2/en
Priority to US14/191,868 priority patent/US9279165B2/en
Abandoned legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C21METALLURGY OF IRON
    • C21CPROCESSING OF PIG-IRON, e.g. REFINING, MANUFACTURE OF WROUGHT-IRON OR STEEL; TREATMENT IN MOLTEN STATE OF FERROUS ALLOYS
    • C21C7/00Treating molten ferrous alloys, e.g. steel, not covered by groups C21C1/00 - C21C5/00
    • C21C7/04Removing impurities by adding a treating agent
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/189Enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01055Alpha-N-arabinofuranosidase (3.2.1.55)
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A23L29/35Degradation products of starch, e.g. hydrolysates, dextrins; Enzymatically modified starches
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C5/00Other raw materials for the preparation of beer
    • C12C5/004Enzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00Preparation of wort
    • C12C7/04Preparation or treatment of the mash
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12CBEER; PREPARATION OF BEER BY FERMENTATION; PREPARATION OF MALT FOR MAKING BEER; PREPARATION OF HOPS FOR MAKING BEER
    • C12C7/00Preparation of wort
    • C12C7/14Lautering, i.e. clarifying wort
    • C12C7/16Lautering, i.e. clarifying wort by straining
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477Hemicellulases not provided in a preceding group
    • C12N9/248Xylanases
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008Endo-1,4-beta-xylanase (3.2.1.8)

Definitions

  • M the moisture content of the grist, in % (m/m)
  • endoglucanases were studied by analyzing degradation products upon incubation with barley beta-glucan. Eppendorf tubes with 0.1 and 10 microgram/ml purified enzyme and 5 mg/ml barley beta-glucan (Megazyme, low viscosity) in 50 mM sodium acetate, 0.01% Triton X-100 at pH 5.5 were incubated in an Eppendorf thermomixer at 50° C. with agitation.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Animal Husbandry (AREA)
  • Metallurgy (AREA)
  • Materials Engineering (AREA)
  • Nutrition Science (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Distillation Of Fermentation Liquor, Processing Of Alcohols, Vinegar And Beer (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Soy Sauces And Products Related Thereto (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Medicines Containing Plant Substances (AREA)
US10/583,676 2003-12-19 2004-12-17 Mashing process Abandoned US20070148741A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US12/493,587 US8679791B2 (en) 2003-12-19 2009-06-29 Mashing process
US14/191,868 US9279165B2 (en) 2003-12-19 2014-02-27 Mashing process

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200301895 2003-12-19
DKPA200301895 2003-12-19
PCT/DK2004/000880 WO2005059084A1 (en) 2003-12-19 2004-12-17 Mashing process

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2004/000880 A-371-Of-International WO2005059084A1 (en) 2003-12-19 2004-12-17 Mashing process

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/493,587 Continuation US8679791B2 (en) 2003-12-19 2009-06-29 Mashing process

Publications (1)

Publication Number Publication Date
US20070148741A1 true US20070148741A1 (en) 2007-06-28

Family

ID=34684454

Family Applications (3)

Application Number Title Priority Date Filing Date
US10/583,676 Abandoned US20070148741A1 (en) 2003-12-19 2004-12-17 Mashing process
US12/493,587 Active 2026-07-05 US8679791B2 (en) 2003-12-19 2009-06-29 Mashing process
US14/191,868 Active US9279165B2 (en) 2003-12-19 2014-02-27 Mashing process

Family Applications After (2)

Application Number Title Priority Date Filing Date
US12/493,587 Active 2026-07-05 US8679791B2 (en) 2003-12-19 2009-06-29 Mashing process
US14/191,868 Active US9279165B2 (en) 2003-12-19 2014-02-27 Mashing process

Country Status (9)

Country Link
US (3) US20070148741A1 (de)
EP (2) EP1706477B1 (de)
CN (4) CN102220192B (de)
BR (1) BRPI0417686B1 (de)
DK (1) DK1706477T3 (de)
MX (1) MXPA06006748A (de)
PL (1) PL1706477T3 (de)
RU (1) RU2376347C2 (de)
WO (1) WO2005059084A1 (de)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012131475A1 (en) * 2011-04-01 2012-10-04 Natural Brew Inc. Producing beer using a wort concentrate
JP2013236580A (ja) * 2012-05-14 2013-11-28 Sapporo Breweries Ltd 植物原料液及び飲料並びにこれらに関する方法
US9279165B2 (en) 2003-12-19 2016-03-08 Novozymes A/S Mashing process
US20160215244A1 (en) * 2013-09-05 2016-07-28 Novozymes A/S Method for Production of Brewers Wort

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CA2586779A1 (en) * 2004-06-03 2005-12-15 Novozymes A/S Mashing process and enzyme composition useful therein
EP2336309A3 (de) 2006-03-20 2011-08-10 Novozymes A/S Polypeptide mit Endoglucanase Aktivität und dafür codierende Polynucleotide.
UA98618C2 (ru) * 2006-05-19 2012-06-11 Хейнекен Сеплай Чейн Б.В. Способ изготовления напитка на основе дрожжевого брожения
US8765199B2 (en) 2006-06-15 2014-07-01 Novozymes A/S Mashing process
BRPI0819869B1 (pt) * 2007-12-12 2019-07-16 Novozymes A/S Processo enzimático para a produção de um mosto de cervejeiro a partir de cereal não maltado, e, uso de um processo para produção de cerveja
CN101918525A (zh) * 2007-12-12 2010-12-15 诺维信公司 糖化方法
DE102008060446A1 (de) * 2008-12-04 2010-06-10 Krones Ag Verfahren zum Ermitteln der Filtrierbarkeit von Bier
US20120135465A1 (en) * 2009-04-08 2012-05-31 Bergsma Martien H Endoglucanase For Reducing The Viscosity Of A Plant Materials Slurry
WO2010129287A2 (en) 2009-04-27 2010-11-11 The Board Of Trustees Of The University Of Illinois Hemicellulose-degrading enzymes
EA025703B1 (ru) * 2009-05-07 2017-01-30 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС Ферментный комплекс для получения пивоваренного затора
ES2574054T3 (es) 2009-11-06 2016-06-14 Novozymes, Inc. Polipéptidos con actividad de xilanasa y polinucleótidos que los codifican
CN102869780A (zh) * 2009-12-21 2013-01-09 诺维信公司 用于自含木素纤维素材料产生发酵产物的方法
US20120276585A1 (en) * 2009-12-21 2012-11-01 Cofco Corporation Method for producing fermentation products from lignocellulose-containing material
EP2655609A4 (de) * 2010-12-21 2015-06-03 Codexis Inc Endoglucanasevarianten
BR112013020264A2 (pt) * 2011-02-09 2016-09-20 Novozymes As composição de eliminação de boboto, uso da composição de eliminação de borboto, e, micróbio geneticamente modificado
EP3061816A1 (de) * 2011-09-14 2016-08-31 DuPont Nutrition Biosciences ApS Verwendung von enzymen mit endo-1,4-xylanaseactivität zur verminderung von geschmacksfehlern in produkte
JP6003077B2 (ja) * 2012-02-15 2016-10-05 株式会社豊田中央研究所 糖加水分解酵素の細胞外発現の増強剤及びその利用
RU2014150072A (ru) 2012-05-11 2016-07-10 Новозимс А/С Способ пивоварения
WO2014005499A1 (en) * 2012-07-02 2014-01-09 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
AU2012387042A1 (en) * 2012-08-03 2015-02-19 Dupont Nutrition Biosciences Aps Enzymes
CA2880776A1 (en) * 2012-08-03 2014-02-06 Dupont Nutrition Biosciences Aps Method
EP2880157B1 (de) * 2012-08-03 2019-03-06 DuPont Nutrition Biosciences ApS Xylanasen zur solubilisierung von arabinoxylanhaltigem material
US10240137B2 (en) 2013-03-14 2019-03-26 Agrivida, Inc. Use of dimerization domains for temperature regulation of enzyme activity
ES2757053T3 (es) 2014-07-10 2020-04-28 Novozymes As Polipéptidos con actividad de xilanasa y polinucleótidos que codifican los mismos
CA2950491A1 (en) * 2014-07-10 2016-01-14 Novozymes A/S Process for producing ethanol from starch using a gh5 xylanase
EP3017706A1 (de) 2014-11-05 2016-05-11 Dupont Nutrition Biosciences ApS Enzyme zum Mälzen
WO2016095856A1 (en) * 2014-12-19 2016-06-23 Novozymes A/S Compositions comprising polypeptides having xylanase activity and polypeptides having arabinofuranosidase activity
ES2935639T3 (es) 2015-11-26 2023-03-08 Novozymes As Proceso de molienda
NZ748105A (en) 2016-05-02 2020-08-28 Carlsberg Breweries As Beverages containing barley beta-glucan
CN114507700A (zh) 2016-11-25 2022-05-17 诺维信公司 Gh10木聚糖酶、gh62阿拉伯呋喃糖苷酶、研磨方法以及其他应用
WO2018127486A1 (en) 2017-01-03 2018-07-12 Novozymes A/S Enzymatic dehusking of pulses
WO2019121930A1 (en) 2017-12-20 2019-06-27 Dsm Ip Assets B.V. Animal feed compositions and uses thereof
EP3530743A1 (de) 2018-02-21 2019-08-28 Cambridge Glycoscience Ltd Verfahren zur herstellung
CA3109239A1 (en) 2018-08-15 2020-02-20 Cambridge Glycoscience Ltd Novel compositions, their use, and methods for their formation
US20220054600A1 (en) 2018-09-17 2022-02-24 Dsm Ip Assets B.V. Animal feed compositions and uses thereof
JP2022545650A (ja) 2019-08-16 2022-10-28 ケンブリッジ グリコサイエンス エルティーディー バイオマスを処理してオリゴ糖および関連組成物を生成する方法
CN110760399A (zh) * 2019-11-29 2020-02-07 江南大学 一种阿拉伯呋喃糖苷酶在啤酒生产中的应用
WO2021116437A2 (en) 2019-12-12 2021-06-17 Cambridge Glycoscience Ltd Low sugar multiphase foodstuffs

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US4746517A (en) * 1985-12-03 1988-05-24 Gist-Brocades S.A. Production of beer
US6197564B1 (en) * 1993-03-10 2001-03-06 Novo Nordisk A/S Enzymes with xylanase activity from Aspergillus aculeatus
US6562340B1 (en) * 1993-12-17 2003-05-13 Genencor International, Inc. Enzyme feed additive and animal feed including it

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US4110163A (en) * 1973-02-28 1978-08-29 Novo Industri A/S Beta-glucanase
US4746517A (en) * 1985-12-03 1988-05-24 Gist-Brocades S.A. Production of beer
US6197564B1 (en) * 1993-03-10 2001-03-06 Novo Nordisk A/S Enzymes with xylanase activity from Aspergillus aculeatus
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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9279165B2 (en) 2003-12-19 2016-03-08 Novozymes A/S Mashing process
WO2012131475A1 (en) * 2011-04-01 2012-10-04 Natural Brew Inc. Producing beer using a wort concentrate
GB2503633A (en) * 2011-04-01 2014-01-01 Natural Brew Inc Producing beer using a wort concentrate
GB2503633B (en) * 2011-04-01 2019-04-17 Natural Brew Inc Producing beer using a wort concentrate
JP2013236580A (ja) * 2012-05-14 2013-11-28 Sapporo Breweries Ltd 植物原料液及び飲料並びにこれらに関する方法
US20160215244A1 (en) * 2013-09-05 2016-07-28 Novozymes A/S Method for Production of Brewers Wort

Also Published As

Publication number Publication date
EP1706477A1 (de) 2006-10-04
US20090269818A1 (en) 2009-10-29
CN102220192A (zh) 2011-10-19
CN102220193A (zh) 2011-10-19
BRPI0417686A (pt) 2007-03-20
EP2258829A2 (de) 2010-12-08
PL1706477T3 (pl) 2017-06-30
CN102220191A (zh) 2011-10-19
BRPI0417686B1 (pt) 2019-12-10
MXPA06006748A (es) 2006-09-04
US9279165B2 (en) 2016-03-08
CN102220191B (zh) 2014-05-21
RU2376347C2 (ru) 2009-12-20
US8679791B2 (en) 2014-03-25
CN1918277A (zh) 2007-02-21
RU2006126098A (ru) 2008-01-27
CN102220192B (zh) 2013-04-17
WO2005059084A1 (en) 2005-06-30
EP2258829A3 (de) 2013-03-27
US20140170712A1 (en) 2014-06-19
DK1706477T3 (en) 2017-01-23
EP1706477B1 (de) 2016-10-05

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