UA41292C2 - Біологічний спосіб одержання 7-амінодезацетоксицефалоспоранової кислоти (7-адса) - Google Patents

Біологічний спосіб одержання 7-амінодезацетоксицефалоспоранової кислоти (7-адса)

Info

Publication number
UA41292C2
UA41292C2 UA94005434A UA94005434A UA41292C2 UA 41292 C2 UA41292 C2 UA 41292C2 UA 94005434 A UA94005434 A UA 94005434A UA 94005434 A UA94005434 A UA 94005434A UA 41292 C2 UA41292 C2 UA 41292C2
Authority
UA
Ukraine
Prior art keywords
adipoyl
adca
mentioned
obtaining
acid
Prior art date
Application number
UA94005434A
Other languages
English (en)
Russian (ru)
Inventor
Майкл Дж. Кондер
Лорілі Крофорд
Лорили Крофорд
Філліс К. Макейда
Филлис К. Макейда
Джон А. Рембоусек
Original Assignee
Дсм Н.В.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Дсм Н.В. filed Critical Дсм Н.В.
Publication of UA41292C2 publication Critical patent/UA41292C2/uk

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/02—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by desacylation of the substituent in the 7 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
    • C12P37/04—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin by acylation of the substituent in the 6 position
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/933—Penicillium
    • Y10S435/935—Penicillium chrysogenum

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Cephalosporin Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)

Abstract

Біологічний спосіб одержання 7-амінодезацетоксицефалоспоранової кислоти (7-ADCA) включає наступні стадії: а) підтримка штаму Penicillium chrysogenum, який продукує ізопеніцилін N, в культуральному середовищі, здатному забезпечувати його зростання. Додавання в зазначене культуральне середовище адипінат-вмісної сировини, що містить адипінову кислоту або одну або декілька її солей і складних ефірів, які можуть засвоюватися і утилізуватися зазначеним штамом Penicillium chrysogenum з продукуванням адипоіл-6-амінопеніцилінової кислоти (адипоіл-6-АРА), за допомогою чого продукується зазначена адипоіл-6-АРА; б) взаємодія зазначеної адипоіл-7-ADCA з адипоілацилазою, за допомогою чого усувається адипоільний бічний ланцюг і утворюється 7-ADCA продукту. Зазначений продукт далі виділяють.
UA94005434A 1991-09-11 1992-09-11 Біологічний спосіб одержання 7-амінодезацетоксицефалоспоранової кислоти (7-адса) UA41292C2 (uk)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US75787991A 1991-09-11 1991-09-11
US07/933,469 US5318896A (en) 1991-09-11 1992-08-28 Recombinant expandase bioprocess for preparing 7-aminodesacetoxy cephalosporanic acid (7-ADCA)
PCT/US1992/007711 WO1993005158A1 (en) 1991-09-11 1992-09-11 Novel bioprocess for preparing 7-adca

Publications (1)

Publication Number Publication Date
UA41292C2 true UA41292C2 (uk) 2001-09-17

Family

ID=27116462

Family Applications (1)

Application Number Title Priority Date Filing Date
UA94005434A UA41292C2 (uk) 1991-09-11 1992-09-11 Біологічний спосіб одержання 7-амінодезацетоксицефалоспоранової кислоти (7-адса)

Country Status (25)

Country Link
US (1) US5318896A (uk)
EP (2) EP0843013A3 (uk)
JP (2) JP3027847B2 (uk)
KR (2) KR0132440B1 (uk)
CN (2) CN1066488C (uk)
AT (1) ATE173017T1 (uk)
AU (1) AU657787B2 (uk)
BG (1) BG62177B1 (uk)
CA (1) CA2077921C (uk)
CZ (1) CZ287357B6 (uk)
DE (1) DE69227494T2 (uk)
DK (1) DK0532341T3 (uk)
ES (1) ES2126588T3 (uk)
FI (1) FI108148B (uk)
HU (1) HU217171B (uk)
IL (1) IL103076A (uk)
MX (1) MX9205175A (uk)
NO (1) NO316742B1 (uk)
NZ (1) NZ244236A (uk)
RO (1) RO115886B1 (uk)
RU (1) RU2178808C2 (uk)
SK (1) SK282387B6 (uk)
TW (1) TW270149B (uk)
UA (1) UA41292C2 (uk)
WO (1) WO1993005158A1 (uk)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3789880T2 (de) * 1986-01-31 1994-09-08 Beecham Group Plc Isolierung und Expression von bei der Biosynthese von Beta-Laktamen beteiligten Genen.
US6709859B1 (en) * 1986-01-31 2004-03-23 Beecham Group P.L.C. Chromosomal DNA Fragments Encoding Enzymes for Encoding B-Lactam Biosynthesis Enzymes, and Vector and Transformants for Their Expression
HU219370B (en) * 1991-10-15 2001-03-28 Gist Brocades Bv Novel bioprocesses for preparing 7-aca and 7-adac
JP3574453B2 (ja) * 1993-07-30 2004-10-06 ギスト ブロカデス ベスローテン フェンノートシャップ ３‐（カルボキシエチルチオ）プロピオニル‐７‐ａｄｃａを経由した７‐ａｄｃａの効果的な製造法
DE69434023D1 (de) * 1993-07-30 2004-10-28 Dsm Ip Assets Bv Verfahren zur effizienten Herstellung von 7-ADCA via 2-(carboxyethylthio)acetyl-7-ADCA und 3-(carboxymethylthio)propionyl-7-ADCA
US5731165A (en) * 1995-06-02 1998-03-24 Gist-Brocades, B.V. Process for the production of 7-ADCA via expandase activity on penicillin G
WO1997020053A2 (en) * 1995-11-27 1997-06-05 Gist-Brocades B.V. Improved process for the production of semi-synthetic cephalosporins via expandase activity on penicillin g
AU2508897A (en) * 1996-04-04 1997-10-29 Gist-Brocades B.V. An enzyme with high adipoyl-coenzyme a synthetase activity and uses thereof
WO1998000382A1 (fr) 1996-07-02 1998-01-08 Toray Industries, Inc. Procedes de preparation de cetones optiquement actives
JP2000514301A (ja) 1996-07-16 2000-10-31 ギスト ブロカデス ベスローテン フェンノートシャップ アクレモニウム・クリソゲヌムを用いたセファロスポリンの新規な調製方法
ATE327340T1 (de) * 1997-02-20 2006-06-15 Dsm Ip Assets Bv Fermentative herstellung von wertstoffen in industriellem umfang durch verwendung von chemisch definierten media
WO1998048037A1 (en) * 1997-04-22 1998-10-29 Dsm N.V. A METHOD FOR CONTROLLING THE SOLUBILITY OF A β-LACTAM NUCLEUS
DE69828940T2 (de) 1997-04-22 2005-12-29 Dsm Ip Assets B.V. Verbessertes verfahren zur fermentativen herstellung von cephalosporin
ZA983387B (en) * 1997-04-22 1999-01-26 Gist Brocades Bv A method for preparing a beta-lactam antibiotic
DE69826604D1 (de) * 1997-04-22 2004-11-04 Gist Brocades Bv Verfahren zur fermentativen herstellung von deacylierten cephalosporinen
PL330760A1 (en) * 1997-04-22 1999-05-24 Gist Brocades Bv Method of obatining decylated cephalosporins by fermentation process
CA2316401A1 (en) * 1997-12-24 1999-07-08 Isis Innovation Limited Modified deacetoxycephalosporin c synthase (daocs) and x-ray structure
PT1066294E (pt) 1998-03-27 2003-03-31 Dsm Nv Novo processo para a producao fermentativa de cefalosporina
US6383773B2 (en) * 1998-04-23 2002-05-07 Massachusetts Institute Of Technology Penicillin conversion
ID27054A (id) 1998-05-19 2001-02-22 Dsm Nv Perbaikan produksi sepalosporin di dalam organisme yang hidup
ES2165276B1 (es) * 1999-06-18 2003-03-01 Antibioticos Sau Procedimiento biotecnologico para la produccion de acido 7-aminocefal osporanico (7-adca) y compuestos de sintesis intermedios particularmente penicilina n y desacetoxicefalosporina c (daoc)
CZ304775B6 (cs) 2000-05-13 2014-10-15 Smithkline Beecham Plc Způsob přípravy klavulanátu draselného
EP1974029A2 (en) 2005-12-28 2008-10-01 DSMIP Assets B.V. Mutant acylases
KR20080106522A (ko) * 2006-02-23 2008-12-08 디에스엠 아이피 어셋츠 비.브이. 개선된 세팔로스포린 제조
EP2123772A1 (en) 2008-04-29 2009-11-25 DSM IP Assets B.V. Beta-lactam antibiotic producing strains
CN102131921A (zh) 2008-08-05 2011-07-20 帝斯曼知识产权资产管理有限公司 生产己二酰基-7-adca的菌株
WO2010015625A1 (en) * 2008-08-05 2010-02-11 Dsm Ip Assets B.V. Adipoyl-7-adca producing strains
CN102119213A (zh) * 2008-08-05 2011-07-06 帝斯曼知识产权资产管理有限公司 生产己二酰基-7-adca的菌株
WO2010115838A1 (en) 2009-04-03 2010-10-14 Dsm Ip Assets B.V. Fermentation process
EP2585608B1 (en) 2010-06-22 2017-07-26 DSM Sinochem Pharmaceuticals Netherlands B.V. Air bubble fermentation process
EP2614066B1 (en) 2010-09-07 2017-11-29 DSM Sinochem Pharmaceuticals Netherlands B.V. Process for the production of cephalosporins
MX343543B (es) 2011-03-03 2016-11-08 Dsm Sinochem Pharm Nl Bv Degradación de compuestos de penicilina.
WO2015091455A1 (en) 2013-12-17 2015-06-25 Dpx Holdings B.V. Bioreactor
CN113699209B (zh) * 2021-08-30 2023-05-02 浙江昂利康制药股份有限公司 一种7-adca回收方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1270852A (fr) * 1960-09-20 1961-09-01 Bayer Ag Procédé d'acylation enzymatique d'acide 6-aminopénicillanique
DE3789880T2 (de) * 1986-01-31 1994-09-08 Beecham Group Plc Isolierung und Expression von bei der Biosynthese von Beta-Laktamen beteiligten Genen.
IL81555A0 (en) * 1986-11-17 1987-09-16 Baldwin Jack Edward Enzymatic process for 3-substituted cephalosporins
ES2058141T3 (es) * 1987-01-17 1994-11-01 Hoechst Ag Utilizacion de gamma-glutamil-transpeptidasa.
US5070020A (en) * 1988-05-09 1991-12-03 Eli Lilly And Company Recombinant dna expression vectors and dna compounds that encode deacetoxycephalosporin c synthetase
US5108918A (en) * 1988-08-11 1992-04-28 Gist-Brocades Method for identifying and using biosynthetic genes for enhanced production of secondary metabolites
IL95766A (en) * 1989-09-27 1996-12-05 Lilly Co Eli Recombinant DNA expression vectors And DNA compounds. Which encode Aspergillus acyltransferase activity
KR0171897B1 (ko) * 1989-12-27 1999-02-01 후지사와 도모끼찌로 7-아미노세펨 화합물 또는 그 염류의 제조 방법
HU219370B (en) * 1991-10-15 2001-03-28 Gist Brocades Bv Novel bioprocesses for preparing 7-aca and 7-adac

Also Published As

Publication number Publication date
MX9205175A (es) 1994-02-28
NO316742B1 (no) 2004-04-26
ES2126588T3 (es) 1999-04-01
FI108148B (fi) 2001-11-30
NZ244236A (en) 1994-03-25
EP0532341A1 (en) 1993-03-17
SK28894A3 (en) 1994-09-07
DE69227494T2 (de) 1999-04-08
IL103076A (en) 1996-10-31
DE69227494D1 (de) 1998-12-10
BG62177B1 (bg) 1999-04-30
WO1993005158A1 (en) 1993-03-18
CN1066488C (zh) 2001-05-30
JP2000060582A (ja) 2000-02-29
EP0532341B1 (en) 1998-11-04
RU2178808C2 (ru) 2002-01-27
EP0843013A3 (en) 1998-06-17
DK0532341T3 (da) 1999-07-19
RO115886B1 (ro) 2000-07-28
JP3027847B2 (ja) 2000-04-04
SK282387B6 (sk) 2002-01-07
NO940848D0 (no) 1994-03-10
KR0132440B1 (ko) 1998-04-14
HU217171B (hu) 1999-12-28
CA2077921A1 (en) 1993-03-12
CZ53294A3 (en) 1994-08-17
BG98643A (bg) 1995-03-31
TW270149B (uk) 1996-02-11
AU657787B2 (en) 1995-03-23
JPH07501931A (ja) 1995-03-02
US5318896A (en) 1994-06-07
EP0843013A2 (en) 1998-05-20
HUT69801A (en) 1995-09-28
ATE173017T1 (de) 1998-11-15
NO940848L (no) 1994-03-10
AU2354292A (en) 1993-03-18
CZ287357B6 (cs) 2000-11-15
CN1332247A (zh) 2002-01-23
FI941135A0 (fi) 1994-03-10
KR940702544A (ko) 1994-08-20
HU9400715D0 (en) 1994-06-28
CN1075336A (zh) 1993-08-18
IL103076A0 (en) 1993-02-21
CA2077921C (en) 1998-11-24
FI941135A (fi) 1994-03-10
JP3072101B2 (ja) 2000-07-31

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