TWI832364B - 重組蛋白之有效選擇性 - Google Patents

重組蛋白之有效選擇性 Download PDF

Info

Publication number
TWI832364B
TWI832364B TW111129504A TW111129504A TWI832364B TW I832364 B TWI832364 B TW I832364B TW 111129504 A TW111129504 A TW 111129504A TW 111129504 A TW111129504 A TW 111129504A TW I832364 B TWI832364 B TW I832364B
Authority
TW
Taiwan
Prior art keywords
seq
cell
gene
protein
cells
Prior art date
Application number
TW111129504A
Other languages
English (en)
Chinese (zh)
Other versions
TW202246506A (zh
Inventor
狄柏麗 德薛潘德
達瑞亞 布拉科夫
剛 陳
詹姆斯 凡德爾
Original Assignee
美商再生元醫藥公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 美商再生元醫藥公司 filed Critical 美商再生元醫藥公司
Publication of TW202246506A publication Critical patent/TW202246506A/zh
Application granted granted Critical
Publication of TWI832364B publication Critical patent/TWI832364B/zh

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
TW111129504A 2014-08-19 2015-08-17 重組蛋白之有效選擇性 TWI832364B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462039416P 2014-08-19 2014-08-19
US62/039,416 2014-08-19

Publications (2)

Publication Number Publication Date
TW202246506A TW202246506A (zh) 2022-12-01
TWI832364B true TWI832364B (zh) 2024-02-11

Family

ID=55347784

Family Applications (3)

Application Number Title Priority Date Filing Date
TW111129504A TWI832364B (zh) 2014-08-19 2015-08-17 重組蛋白之有效選擇性
TW104126657A TWI719945B (zh) 2014-08-19 2015-08-17 重組蛋白之有效選擇性
TW110101888A TWI777374B (zh) 2014-08-19 2015-08-17 重組蛋白之有效選擇性

Family Applications After (2)

Application Number Title Priority Date Filing Date
TW104126657A TWI719945B (zh) 2014-08-19 2015-08-17 重組蛋白之有效選擇性
TW110101888A TWI777374B (zh) 2014-08-19 2015-08-17 重組蛋白之有效選擇性

Country Status (27)

Country Link
US (4) US9732357B2 (enExample)
EP (2) EP3183359B1 (enExample)
JP (4) JP7115851B2 (enExample)
KR (2) KR102556383B1 (enExample)
CN (2) CN113293139B (enExample)
AR (1) AR102333A1 (enExample)
AU (3) AU2015305565B9 (enExample)
CA (1) CA2957036A1 (enExample)
CY (1) CY1124079T1 (enExample)
DK (1) DK3183359T3 (enExample)
EA (1) EA035340B1 (enExample)
ES (1) ES2849728T3 (enExample)
HR (1) HRP20210301T1 (enExample)
HU (1) HUE054069T2 (enExample)
IL (3) IL287020B (enExample)
LT (1) LT3183359T (enExample)
MA (1) MA40371B1 (enExample)
MX (2) MX384990B (enExample)
MY (1) MY191745A (enExample)
PL (1) PL3183359T3 (enExample)
PT (1) PT3183359T (enExample)
RS (1) RS61371B1 (enExample)
SG (2) SG11201701121UA (enExample)
SI (1) SI3183359T1 (enExample)
SM (1) SMT202100054T1 (enExample)
TW (3) TWI832364B (enExample)
WO (1) WO2016028838A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR102333A1 (es) 2014-08-19 2017-02-22 Regeneron Pharma Selectividad eficiente de proteínas recombinadas
WO2019157395A1 (en) 2018-02-08 2019-08-15 Applied Stemcells, Inc Methods for screening variant of target gene
CN114585597A (zh) 2019-10-23 2022-06-03 佳能株式会社 制造陶瓷制品的方法和陶瓷制品

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009066919A2 (en) * 2007-11-23 2009-05-28 Korea Research Institute Of Bioscience And Biotechnology Gpt gene as a marker for selecting plant transformant

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4634665A (en) 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US6077835A (en) 1994-03-23 2000-06-20 Case Western Reserve University Delivery of compacted nucleic acid to cells
KR970029803A (ko) 1995-11-03 1997-06-26 김광호 반도체 메모리장치의 프리차지 회로
US5972650A (en) 1997-06-26 1999-10-26 Brigham And Women's Hospital Tetracycline repressor regulated mammalian cell transcription and viral replication switch
US6281005B1 (en) 1999-05-14 2001-08-28 Copernicus Therapeutics, Inc. Automated nucleic acid compaction device
WO2001053312A1 (en) * 1999-12-23 2001-07-26 Hyseq, Inc. Novel nucleic acids and polypeptides
EP1392859B1 (en) 2001-01-16 2006-05-10 Regeneron Pharmaceuticals, Inc. Isolating cells expressing secreted proteins
US7192737B2 (en) 2002-03-29 2007-03-20 Xoma Technology Ltd. Methods and materials for increasing expression of recombinant polypeptides
US8673589B2 (en) 2002-05-29 2014-03-18 Regeneron Pharmaceuticals, Inc. Inducible eukaryotic expression system
US7384744B2 (en) * 2002-11-29 2008-06-10 Boehringer Ingelheim Pharma Gmbh & Co., Kg Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products
JP2005027599A (ja) 2003-07-09 2005-02-03 Nara Institute Of Science & Technology 植物由来遺伝子を用いた遺伝子導入植物の選抜法
EP1586585A1 (de) * 2004-04-14 2005-10-19 F. Hoffmann-La Roche Ag Expressionssystem zur Herstellung von IL-15/Fc-Fusionsproteinen und ihre Verwendung
AR064200A1 (es) 2006-12-07 2009-03-18 Dow Agrosciences Llc Genes marcadores seleccionables
ES2522615T3 (es) 2007-06-04 2014-11-17 Regeneron Pharmaceuticals, Inc. Regiones de expresión y estabilidad potenciadas
ES2570853T3 (es) * 2007-12-07 2016-05-20 Zymogenetics Inc Moléculas de anticuerpo humanizadas específicas para IL-31
US20110091495A1 (en) * 2008-04-22 2011-04-21 Rutgers, The State University Of New Jersey Hcv e2 construct compositions and methods
US8927249B2 (en) * 2008-12-09 2015-01-06 Halozyme, Inc. Extended soluble PH20 polypeptides and uses thereof
EP2674495A1 (en) 2012-06-14 2013-12-18 Sanofi CHO expression system
AR102333A1 (es) 2014-08-19 2017-02-22 Regeneron Pharma Selectividad eficiente de proteínas recombinadas

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009066919A2 (en) * 2007-11-23 2009-05-28 Korea Research Institute Of Bioscience And Biotechnology Gpt gene as a marker for selecting plant transformant

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
期刊 Scocca JR et al., " Sequence of a cDNA That Specifies the Uridine Diphosphate IV-Acetylo-glucosamine:Dolichol Phosphate N- Acetylglucosamine- 1 -phosphateTransferase from Chinese Hamster Ovary Cells" J Biol Chem., vol.265,no.33, the American Society for Biochemistry and Molecular Biology, 1990 Nov 25, p:20621-6. *

Also Published As

Publication number Publication date
HRP20210301T1 (hr) 2021-04-02
AU2015305565B9 (en) 2020-05-21
TW202124716A (zh) 2021-07-01
KR20220080215A (ko) 2022-06-14
MA40371B1 (fr) 2021-03-31
JP7115851B2 (ja) 2022-08-09
SMT202100054T1 (it) 2021-03-15
AU2023200752A1 (en) 2023-03-09
BR112017003074A2 (pt) 2018-03-06
MY191745A (en) 2022-07-13
DK3183359T3 (da) 2021-02-01
EP3901274A1 (en) 2021-10-27
MA40371A (fr) 2017-06-28
AU2023200752B2 (en) 2024-02-29
IL287020B (en) 2022-09-01
CN107075549A (zh) 2017-08-18
TW201615833A (zh) 2016-05-01
TWI777374B (zh) 2022-09-11
US10457959B2 (en) 2019-10-29
CA2957036A1 (en) 2016-02-25
IL295361B1 (en) 2023-04-01
EA201790241A1 (ru) 2017-06-30
IL250531A0 (en) 2017-03-30
IL287020A (en) 2021-12-01
US20200063159A1 (en) 2020-02-27
CN113293139A (zh) 2021-08-24
IL295361A (en) 2022-10-01
AU2020205336A1 (en) 2020-08-06
IL250531B (en) 2021-10-31
JP2017525368A (ja) 2017-09-07
US9732357B2 (en) 2017-08-15
SI3183359T1 (sl) 2021-03-31
AU2020205336B2 (en) 2022-11-10
SG11201701121UA (en) 2017-03-30
US20160053280A1 (en) 2016-02-25
MX2017002203A (es) 2017-05-22
JP2024166420A (ja) 2024-11-28
US20210340564A1 (en) 2021-11-04
IL295361B2 (en) 2023-08-01
ES2849728T3 (es) 2021-08-20
CN113293139B (zh) 2025-04-29
EP3183359A4 (en) 2018-04-04
CN107075549B (zh) 2021-04-20
JP7260510B2 (ja) 2023-04-18
JP2023076667A (ja) 2023-06-01
LT3183359T (lt) 2021-02-25
PL3183359T3 (pl) 2021-08-02
JP7561232B2 (ja) 2024-10-03
KR102406976B1 (ko) 2022-06-10
MX384990B (es) 2025-03-14
EP3183359B1 (en) 2020-12-16
AU2015305565B2 (en) 2020-04-30
JP2020174681A (ja) 2020-10-29
MX2021009154A (es) 2021-09-10
PT3183359T (pt) 2021-02-03
EP3183359A1 (en) 2017-06-28
SG10201707859RA (en) 2017-10-30
US20170335341A1 (en) 2017-11-23
CY1124079T1 (el) 2022-05-27
RS61371B1 (sr) 2021-02-26
EA035340B1 (ru) 2020-05-29
AR102333A1 (es) 2017-02-22
KR102556383B1 (ko) 2023-07-17
KR20170055481A (ko) 2017-05-19
US11085053B2 (en) 2021-08-10
TWI719945B (zh) 2021-03-01
WO2016028838A1 (en) 2016-02-25
TW202246506A (zh) 2022-12-01
HUE054069T2 (hu) 2021-08-30
AU2015305565A1 (en) 2017-03-09

Similar Documents

Publication Publication Date Title
JP7561232B2 (ja) 組み換え型タンパク質の効率的な選択性
EP3262168B1 (en) Host cell protein modification
JP2022529063A (ja) 安定的な標的組み込み
HK40055450A (en) Efficient selectivity of recombinant proteins
HK1240271A1 (en) Efficient selectivity of recombinant proteins
HK1240271B (en) Efficient selectivity of recombinant proteins
US20040209323A1 (en) Protein expression by codon harmonization and translational attenuation
BR112017003074B1 (pt) Vetor, e, métodos de produção de uma proteína recombinante de interesse e de emprego de tunicamicina como um marcador de seleção
TW202525834A (zh) 構建生產去岩藻糖基化蛋白質的細胞的方法及其應用
Cartwright CHO Cell Genetic Instability: From Transfection to Stable Cell Line