TWI697560B - 用於偵測細菌污染之方法及組合物 - Google Patents

用於偵測細菌污染之方法及組合物 Download PDF

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TWI697560B
TWI697560B TW103125440A TW103125440A TWI697560B TW I697560 B TWI697560 B TW I697560B TW 103125440 A TW103125440 A TW 103125440A TW 103125440 A TW103125440 A TW 103125440A TW I697560 B TWI697560 B TW I697560B
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seq
primer
sequence
complement
probe
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TW103125440A
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Chinese (zh)
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TW201525145A (zh
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建全 王
相民 崔
曉嵐 陳
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美商Dch分子診斷公司
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/15—Modifications characterised by incorporating a consensus or conserved sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
TW103125440A 2013-07-25 2014-07-24 用於偵測細菌污染之方法及組合物 TWI697560B (zh)

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US201361858495P 2013-07-25 2013-07-25
US61/858,495 2013-07-25

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TW201525145A TW201525145A (zh) 2015-07-01
TWI697560B true TWI697560B (zh) 2020-07-01

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US (2) US10392670B2 (cg-RX-API-DMAC10.html)
EP (1) EP3024947B1 (cg-RX-API-DMAC10.html)
JP (3) JP2016525359A (cg-RX-API-DMAC10.html)
KR (1) KR102245110B1 (cg-RX-API-DMAC10.html)
CN (2) CN105473737B (cg-RX-API-DMAC10.html)
AU (1) AU2014293075A1 (cg-RX-API-DMAC10.html)
CA (1) CA2917430C (cg-RX-API-DMAC10.html)
MX (1) MX2016000997A (cg-RX-API-DMAC10.html)
SG (1) SG11201600550WA (cg-RX-API-DMAC10.html)
TW (1) TWI697560B (cg-RX-API-DMAC10.html)
WO (1) WO2015013465A2 (cg-RX-API-DMAC10.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12529106B2 (en) 2023-06-09 2026-01-20 Inti Taiwan, Inc. Assays and methods to assist in vitro fertilization

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015013465A2 (en) 2013-07-25 2015-01-29 Dch Molecular Diagnostics, Inc. Methods and compositions for detecting bacterial contamination
US20160244812A1 (en) * 2013-10-07 2016-08-25 Mitsui Chemicals, Inc. Primer set for pcr for bacterial dna amplification, kit for detection and/or identification of bacterial species, and, method of detection and/or identification of bacterial species
US9953415B2 (en) * 2014-12-03 2018-04-24 Tata Consultancy Services Limited System and method for quantification of Escherichia coli bacteria in water
CA2995468A1 (en) * 2015-08-12 2017-02-16 Circulogene Theranostics, Llc Method of preparing cell free nucleic acid molecules by in situ amplification
CN105368961A (zh) * 2015-12-15 2016-03-02 天津脉络生物科技有限公司 一种沙雷菌定量检测荧光pcr试剂盒及其应用
KR102326769B1 (ko) * 2016-03-25 2021-11-17 카리우스, 인코포레이티드 합성 핵산 스파이크-인
CN105734164B (zh) * 2016-05-06 2019-05-17 苏州大学 一种检测细菌性脑膜炎病原体的多重pcr试剂盒
CN106434890B (zh) * 2016-08-30 2020-02-21 上海生物信息技术研究中心 快速恒温检测小肠结肠炎耶尔森氏菌的方法、引物及试剂盒
US20190062809A1 (en) * 2017-08-24 2019-02-28 Clinical Micro Sensors, Inc. (dba GenMark Diagnostics, Inc.) Electrochemical detection of bacterial and/or fungal infections
EP3706574A4 (en) * 2017-11-06 2021-08-25 The Regents of The University of Michigan COMPOSITIONS AND METHODS FOR DETECTION OF MICRO-ORGANISMS
JP2021502825A (ja) * 2017-11-13 2021-02-04 ライフ テクノロジーズ コーポレイション 尿路微生物検出のための組成物、方法、およびキット
US12291745B2 (en) * 2018-01-30 2025-05-06 Biofire Defense, Llc Methods and systems for validation of a nucleic acid amplification assay
CN108148834B (zh) * 2018-03-14 2018-11-13 北京理工大学 用于检测空间环境中的真菌的引物组合、试剂盒及方法
WO2019200134A2 (en) * 2018-04-13 2019-10-17 National Agricultural Genotyping Center Universal lactic acid bacteria quantification kits, methods, compositions and apparatuses therefor
US11473133B2 (en) * 2018-09-24 2022-10-18 Second Genome, Inc. Methods for validation of microbiome sequence processing and differential abundance analyses via multiple bespoke spike-in mixtures
CN109337967A (zh) * 2018-09-27 2019-02-15 华中科技大学鄂州工业技术研究院 一种实验室的微生物污染鉴别方法
FR3097560B1 (fr) * 2019-06-24 2021-06-25 Oreal Méthode de diagnostic d’une exposition chronique à la pollution
CN111209895B (zh) * 2020-02-14 2022-03-25 李淑媛 静脉药物配制样品的细菌污染检测系统、方法及存储介质
CN112575097B (zh) * 2020-10-12 2022-05-10 南开大学 一种用于检测蜡样芽孢杆菌的液相芯片及应用
WO2022081815A1 (en) * 2020-10-14 2022-04-21 Pickens Peter Vincent Optimized detection of blood-borne microbes
JP7553323B2 (ja) 2020-10-30 2024-09-18 株式会社Kri スタフィロコッカス細菌の同定方法
CN112226528B (zh) * 2020-12-14 2021-03-16 臻和(北京)生物科技有限公司 一种用于生物组织样本检测细菌污染的质检方法
CN116745434A (zh) * 2021-01-20 2023-09-12 保障生物系统控股有限公司 基因组序列的改进的检测及其探针分子
WO2023034618A1 (en) * 2021-09-03 2023-03-09 Micronoma, Inc. Methods of identifying cancer-associated microbial biomarkers
CN114480596A (zh) * 2022-02-11 2022-05-13 宁波市中心血站 一种微流控血液及血制品细菌污染检测方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0692540A2 (en) * 1994-06-17 1996-01-17 Becton, Dickinson and Company Oligonucleotide primers and probes for detection of bacteria
WO2002010444A1 (en) 2000-07-28 2002-02-07 University Of Sydney A method of detecting microorganisms

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
WO1991001384A1 (en) 1989-07-11 1991-02-07 Gen-Probe, Incorporated Nucleic acid sequence amplification methods utilizing a transcription complex
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5270184A (en) 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
EP0728218B1 (en) 1993-11-12 2008-01-02 PHRI Properties, Inc. Hybridization probes for nucleic acid detection, universal stems, methods and kits
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
US5641658A (en) 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US5705628A (en) 1994-09-20 1998-01-06 Whitehead Institute For Biomedical Research DNA purification and isolation using magnetic particles
US5705365A (en) 1995-06-07 1998-01-06 Gen-Probe Incorporated Kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5710029A (en) 1995-06-07 1998-01-20 Gen-Probe Incorporated Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
US20100267012A1 (en) * 1997-11-04 2010-10-21 Bergeron Michel G Highly conserved genes and their use to generate probes and primers for detection of microorganisms
EP2327797B1 (en) 1997-04-01 2015-11-25 Illumina Cambridge Limited Method of nucleic acid sequencing
AU9220498A (en) 1997-09-04 1999-03-22 Bayer Corporation Oligonucleotide probes bearing quenchable fluorescent labels, and methods of usethereof
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
EP1020534B2 (en) 1998-11-09 2011-01-05 Eiken Kagaku Kabushiki Kaisha Process for synthesizing nucleic acid
ATE262595T1 (de) 1999-09-13 2004-04-15 Nugen Technologies Inc Methoden und zusammensetzungen zur linearen isothermalen amplifikation von polynukleotidsequenzen
CA2937907C (en) * 1999-09-28 2017-08-22 Geneohm Sciences Canada Inc. Nucleic acids, methods and kits for the detection of campylobacter
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
US7001724B1 (en) 2000-11-28 2006-02-21 Applera Corporation Compositions, methods, and kits for isolating nucleic acids using surfactants and proteases
AU2003272438B2 (en) 2002-09-20 2009-04-02 New England Biolabs, Inc. Helicase dependent amplification of nucleic acids
EP1565576A2 (en) 2002-11-20 2005-08-24 Pharmacia Italia S.p.A. Method for the detection of microorganisms in pharmaceutical products
CA2508720A1 (en) 2002-12-03 2004-06-17 Dartmouth College Method to measure a t cell response and its uses to qualify antigen-presenting cells
EP1473370A3 (en) * 2003-04-24 2005-03-09 BioMerieux, Inc. Genus, group, species and/or strain specific 16S rDNA Sequences
CA2562221A1 (en) * 2005-10-21 2007-04-21 Canadian Blood Services A method for detection of staphylococcus epidermidis
JP2010524454A (ja) 2007-04-19 2010-07-22 モレキュラー ディテクション インコーポレーテッド 抗生物質耐性細菌の検出および分析のための方法、組成物、およびキット
BR112012009023A2 (pt) * 2009-10-22 2019-09-24 Biotools Biotechnologial & Medical Laboratories S A método pra detecção. diferenciação e identificação de eubactérias específica de táxons em uma amostra biológica por meio de técnicas de análise de sequenciamento e kit
WO2012071135A2 (en) 2010-11-23 2012-05-31 Biovec Transfusion, Llc Methods for removing pathogens from a platelet preparation
WO2015013465A2 (en) 2013-07-25 2015-01-29 Dch Molecular Diagnostics, Inc. Methods and compositions for detecting bacterial contamination

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0692540A2 (en) * 1994-06-17 1996-01-17 Becton, Dickinson and Company Oligonucleotide primers and probes for detection of bacteria
WO2002010444A1 (en) 2000-07-28 2002-02-07 University Of Sydney A method of detecting microorganisms

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
Dergousoff SJ, Chilton NB, Association of Different Genetic Types of Francisella-Like Organisms with the Rocky Mountain Wood Tick (Dermacentor andersoni) and the American Dog Tick (Dermacentor variabilis) in Localities Near Their Northern Distributional Limits Appl Environ Microbiol. 2012 Feb; 78(4): 965–971. *
Ishaq SL, Wright ADG, Insight into the bacterial gut microbiome of the North American moose (Alces alces). BMC Microbiol. 2012; 12: 212. *
Ishaq SL, Wright ADG, Insight into the bacterial gut microbiome of the North American moose (Alces alces). BMC Microbiol. 2012; 12: 212. ^&rn^
Jakobsson T, Forsum U, Changes in the predominant human Lactobacillus flora during in vitro fertilization. Ann Clin Microbiol Antimicrob. 2008; 7: 14. *
Jakobsson T, Forsum U, Changes in the predominant human Lactobacillus flora during in vitro fertilization. Ann Clin Microbiol Antimicrob. 2008; 7: 14. ^&rn^
Kajiwara H, Toda M, Mine T, Nakada H, Yamamoto T, Isolation of Fucosyltransferase-Producing Bacteria from Marine Environments. Microbes Environ. 2012 Dec; 27(4): 515–518. *
Mohammadi T, Pietersz RN, Vandenbroucke-Grauls CM, Savelkoul PH, Reesink HW. Detection of bacteria in platelet concentrates: comparison of broad-range real-time 16S rDNA polymerase chain reaction and automated culturing. Transfusion. 2005 May; 45(5): 731-6. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US12529106B2 (en) 2023-06-09 2026-01-20 Inti Taiwan, Inc. Assays and methods to assist in vitro fertilization

Also Published As

Publication number Publication date
JP2016525359A (ja) 2016-08-25
WO2015013465A2 (en) 2015-01-29
EP3024947B1 (en) 2020-10-28
CN110592244A (zh) 2019-12-20
EP3024947A4 (en) 2017-03-15
SG11201600550WA (en) 2016-02-26
KR102245110B1 (ko) 2021-04-27
AU2014293075A1 (en) 2015-12-17
CA2917430A1 (en) 2015-01-29
MX2016000997A (es) 2016-04-19
US10392670B2 (en) 2019-08-27
CN105473737A (zh) 2016-04-06
WO2015013465A3 (en) 2015-04-23
US20200080134A1 (en) 2020-03-12
US11274351B2 (en) 2022-03-15
JP2022023068A (ja) 2022-02-07
KR20160034318A (ko) 2016-03-29
CN105473737B (zh) 2019-10-25
US20160355871A1 (en) 2016-12-08
EP3024947A2 (en) 2016-06-01
TW201525145A (zh) 2015-07-01
HK1219298A1 (en) 2017-03-31
CA2917430C (en) 2020-10-06
JP2019187431A (ja) 2019-10-31

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