CA2917430C - Methods and compositions for detecting bacterial contamination - Google Patents

Methods and compositions for detecting bacterial contamination Download PDF

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Publication number
CA2917430C
CA2917430C CA2917430A CA2917430A CA2917430C CA 2917430 C CA2917430 C CA 2917430C CA 2917430 A CA2917430 A CA 2917430A CA 2917430 A CA2917430 A CA 2917430A CA 2917430 C CA2917430 C CA 2917430C
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Prior art keywords
seq
primer
sequence
amplification
sample
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CA2917430A
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English (en)
French (fr)
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CA2917430A1 (en
Inventor
James Jian Quan Wang
Xiangmin Cui
Hiunam CHAN
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DCH MOLECULAR DIAGNOSTICS Inc
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DCH MOLECULAR DIAGNOSTICS Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/15—Modifications characterised by incorporating a consensus or conserved sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2917430A 2013-07-25 2014-07-23 Methods and compositions for detecting bacterial contamination Active CA2917430C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361858495P 2013-07-25 2013-07-25
US61/858,495 2013-07-25
PCT/US2014/047914 WO2015013465A2 (en) 2013-07-25 2014-07-23 Methods and compositions for detecting bacterial contamination

Publications (2)

Publication Number Publication Date
CA2917430A1 CA2917430A1 (en) 2015-01-29
CA2917430C true CA2917430C (en) 2020-10-06

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Family Applications (1)

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CA2917430A Active CA2917430C (en) 2013-07-25 2014-07-23 Methods and compositions for detecting bacterial contamination

Country Status (11)

Country Link
US (2) US10392670B2 (cg-RX-API-DMAC10.html)
EP (1) EP3024947B1 (cg-RX-API-DMAC10.html)
JP (3) JP2016525359A (cg-RX-API-DMAC10.html)
KR (1) KR102245110B1 (cg-RX-API-DMAC10.html)
CN (2) CN105473737B (cg-RX-API-DMAC10.html)
AU (1) AU2014293075A1 (cg-RX-API-DMAC10.html)
CA (1) CA2917430C (cg-RX-API-DMAC10.html)
MX (1) MX2016000997A (cg-RX-API-DMAC10.html)
SG (1) SG11201600550WA (cg-RX-API-DMAC10.html)
TW (1) TWI697560B (cg-RX-API-DMAC10.html)
WO (1) WO2015013465A2 (cg-RX-API-DMAC10.html)

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CN105734164B (zh) * 2016-05-06 2019-05-17 苏州大学 一种检测细菌性脑膜炎病原体的多重pcr试剂盒
CN106434890B (zh) * 2016-08-30 2020-02-21 上海生物信息技术研究中心 快速恒温检测小肠结肠炎耶尔森氏菌的方法、引物及试剂盒
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CN112575097B (zh) * 2020-10-12 2022-05-10 南开大学 一种用于检测蜡样芽孢杆菌的液相芯片及应用
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CN112226528B (zh) * 2020-12-14 2021-03-16 臻和(北京)生物科技有限公司 一种用于生物组织样本检测细菌污染的质检方法
CN116745434A (zh) * 2021-01-20 2023-09-12 保障生物系统控股有限公司 基因组序列的改进的检测及其探针分子
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Also Published As

Publication number Publication date
JP2016525359A (ja) 2016-08-25
WO2015013465A2 (en) 2015-01-29
EP3024947B1 (en) 2020-10-28
CN110592244A (zh) 2019-12-20
EP3024947A4 (en) 2017-03-15
SG11201600550WA (en) 2016-02-26
KR102245110B1 (ko) 2021-04-27
AU2014293075A1 (en) 2015-12-17
CA2917430A1 (en) 2015-01-29
MX2016000997A (es) 2016-04-19
US10392670B2 (en) 2019-08-27
CN105473737A (zh) 2016-04-06
WO2015013465A3 (en) 2015-04-23
US20200080134A1 (en) 2020-03-12
US11274351B2 (en) 2022-03-15
TWI697560B (zh) 2020-07-01
JP2022023068A (ja) 2022-02-07
KR20160034318A (ko) 2016-03-29
CN105473737B (zh) 2019-10-25
US20160355871A1 (en) 2016-12-08
EP3024947A2 (en) 2016-06-01
TW201525145A (zh) 2015-07-01
HK1219298A1 (en) 2017-03-31
JP2019187431A (ja) 2019-10-31

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