TW201307561A - 使用凝膠滲透層析法純化病毒之方法 - Google Patents

使用凝膠滲透層析法純化病毒之方法 Download PDF

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Publication number
TW201307561A
TW201307561A TW101115116A TW101115116A TW201307561A TW 201307561 A TW201307561 A TW 201307561A TW 101115116 A TW101115116 A TW 101115116A TW 101115116 A TW101115116 A TW 101115116A TW 201307561 A TW201307561 A TW 201307561A
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Taiwan
Prior art keywords
virus
buffer
gel permeation
permeation chromatography
buffered saline
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TW101115116A
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English (en)
Chinese (zh)
Inventor
麥修C 卡非
艾利森 哈格曼
羅克斯那 卡帕迪亞
莎拉 瑟爾
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腫瘤防治生化科技公司
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Publication of TW201307561A publication Critical patent/TW201307561A/zh

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/42Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424Elution mode
    • B01D15/426Specific type of solvent
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02Separating microorganisms from their culture media
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011Details
    • C12N2720/12011Reoviridae
    • C12N2720/12051Methods of production or purification of viral material

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicinal Preparation (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
TW101115116A 2011-04-29 2012-04-27 使用凝膠滲透層析法純化病毒之方法 TW201307561A (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201161480561P 2011-04-29 2011-04-29

Publications (1)

Publication Number Publication Date
TW201307561A true TW201307561A (zh) 2013-02-16

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TW101115116A TW201307561A (zh) 2011-04-29 2012-04-27 使用凝膠滲透層析法純化病毒之方法

Country Status (15)

Country Link
US (1) US20120273424A1 (enExample)
EP (1) EP2702147B1 (enExample)
JP (1) JP6029651B2 (enExample)
KR (2) KR20140024406A (enExample)
CN (1) CN103492557B (enExample)
AU (1) AU2012248082B2 (enExample)
CA (1) CA2832367A1 (enExample)
DK (1) DK2702147T3 (enExample)
EA (1) EA201391605A1 (enExample)
ES (1) ES2822199T3 (enExample)
IL (1) IL228491A (enExample)
MX (1) MX337348B (enExample)
SG (1) SG194449A1 (enExample)
TW (1) TW201307561A (enExample)
WO (1) WO2012145837A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2630012T3 (es) 2010-12-02 2017-08-17 Oncolytics Biotech Inc. Formulaciones virales liofilizadas
EA201390810A1 (ru) * 2010-12-02 2013-09-30 Онколитикс Байотек Инк. Жидкие вирусные составы
BR112015031541A2 (pt) 2013-06-17 2017-07-25 De Staat Der Nederlanden Vert Door De Mini Van Vws Miniie Van Volksgezondheid Welzijn En Sport métodos para o impedimento da agregação de componentes virais
CN112384615A (zh) 2018-07-04 2021-02-19 普罗拜奥根股份公司 用于纯化包膜病毒的方法
CN111812313B (zh) * 2020-06-22 2021-10-08 北京生物制品研究所有限责任公司 铝佐剂吸附型新型冠状病毒灭活疫苗中抗原的解离方法
CN114544815B (zh) * 2022-03-01 2023-10-27 中牧实业股份有限公司 一种山羊痘病毒的定量检测方法
CN116718524B (zh) * 2023-06-26 2025-11-07 西安交通大学 一种基于细胞膜色谱技术的病毒颗粒检测方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5096885A (en) * 1988-04-15 1992-03-17 Genentech, Inc. Human growth hormone formulation
ATE148165T1 (de) * 1989-07-24 1997-02-15 Bayer Ag Stabilisierung von hochgereinigten proteinen
BR9507657A (pt) * 1994-05-16 1997-09-09 Merck & Co Inc Proteina isolada e purificada de papilomavirus recombinante capsideos particulas semelhantes a virus processos a virus processos para produzir a proteina para tratar ou prevenir a infecçao por papilomavirus para produzir uma proteina de capsideo de papilomavirus recombinante montada em um capsideo ou particula semelhante a virus e para induzir uma resposta imune em um animal vertebrado composiçoes farmacêuticas kit para a detecçao de papilomavirus ou anticorpos para papilomavirus e sistema sinte
CA2723040A1 (en) * 1998-02-17 1999-08-19 Schering Corporation Compositions comprising viruses and methods for concentrating virus preparations
JP4087712B2 (ja) 2001-03-16 2008-05-21 オンコリティクス バイオテック, インコーポレイティッド 細胞培養物からウイルスを抽出する方法
ATE427990T1 (de) * 2002-04-30 2009-04-15 Oncolytics Biotech Inc Verbesserte reinigungsmethode fur viren
US7901921B2 (en) * 2004-10-22 2011-03-08 Oncolytics Biotech Inc. Viral purification methods
EP1878791A1 (en) * 2006-07-11 2008-01-16 Bia Separations D.O.O. Method for influenza virus purification
JP5326155B2 (ja) * 2006-08-07 2013-10-30 一般財団法人化学及血清療法研究所 痘瘡生ワクチンの製造方法
DK2132315T3 (en) * 2007-03-12 2015-10-19 Oncolytics Biotech Inc Reoviruses with modified sequences
CN102089324B (zh) * 2007-11-12 2014-04-16 特罗科隆科学有限公司 用于对流行性感冒进行治疗以及诊断的组合物以及方法
US8227577B2 (en) * 2007-12-21 2012-07-24 Hoffman-La Roche Inc. Bivalent, bispecific antibodies
EA201390810A1 (ru) * 2010-12-02 2013-09-30 Онколитикс Байотек Инк. Жидкие вирусные составы
ES2630012T3 (es) * 2010-12-02 2017-08-17 Oncolytics Biotech Inc. Formulaciones virales liofilizadas

Also Published As

Publication number Publication date
IL228491A0 (en) 2013-12-31
JP2014516515A (ja) 2014-07-17
CA2832367A1 (en) 2012-11-01
EP2702147B1 (en) 2020-07-15
WO2012145837A1 (en) 2012-11-01
AU2012248082B2 (en) 2015-08-20
US20120273424A1 (en) 2012-11-01
CN103492557A (zh) 2014-01-01
EP2702147A1 (en) 2014-03-05
KR102151890B1 (ko) 2020-09-03
JP6029651B2 (ja) 2016-11-24
MX337348B (es) 2016-02-29
IL228491A (en) 2017-05-29
CN103492557B (zh) 2015-07-29
EP2702147A4 (en) 2014-11-05
KR20190026059A (ko) 2019-03-12
SG194449A1 (en) 2013-12-30
ES2822199T3 (es) 2021-04-29
EA201391605A1 (ru) 2014-02-28
DK2702147T3 (da) 2020-09-28
AU2012248082A1 (en) 2013-05-09
KR20140024406A (ko) 2014-02-28
MX2013012627A (es) 2014-05-13

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