CN103492557B - 使用凝胶渗透色谱法纯化病毒的方法 - Google Patents

使用凝胶渗透色谱法纯化病毒的方法 Download PDF

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Publication number
CN103492557B
CN103492557B CN201280020376.8A CN201280020376A CN103492557B CN 103492557 B CN103492557 B CN 103492557B CN 201280020376 A CN201280020376 A CN 201280020376A CN 103492557 B CN103492557 B CN 103492557B
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virus
elution buffer
gel permeation
buffered saline
vehicle
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Chinese (zh)
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CN103492557A (zh
Inventor
M.C.科菲
A.黑格尔曼
R.卡帕迪亚
S.塞尔
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Ankolitix Biotechnology Barbados Co
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Oncolytics Biotech Inc
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12051—Methods of production or purification of viral material

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Medicinal Preparation (AREA)
CN201280020376.8A 2011-04-29 2012-04-27 使用凝胶渗透色谱法纯化病毒的方法 Active CN103492557B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161480561P 2011-04-29 2011-04-29
US61/480561 2011-04-29
PCT/CA2012/000406 WO2012145837A1 (en) 2011-04-29 2012-04-27 Methods of purifying viruses using gel permeation chromatography

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CN103492557A CN103492557A (zh) 2014-01-01
CN103492557B true CN103492557B (zh) 2015-07-29

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US (1) US20120273424A1 (enExample)
EP (1) EP2702147B1 (enExample)
JP (1) JP6029651B2 (enExample)
KR (2) KR20140024406A (enExample)
CN (1) CN103492557B (enExample)
AU (1) AU2012248082B2 (enExample)
CA (1) CA2832367A1 (enExample)
DK (1) DK2702147T3 (enExample)
EA (1) EA201391605A1 (enExample)
ES (1) ES2822199T3 (enExample)
IL (1) IL228491A (enExample)
MX (1) MX337348B (enExample)
SG (1) SG194449A1 (enExample)
TW (1) TW201307561A (enExample)
WO (1) WO2012145837A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX373096B (es) * 2010-12-02 2020-04-27 Oncolytics Biotech Inc Formulaciones virales líquidas.
EA201390812A1 (ru) 2010-12-02 2013-11-29 Онколитикс Байотек Инк. Лиофилизированные вирусные составы
PL3010537T3 (pl) * 2013-06-17 2019-03-29 De Staat Der Nederlanden, Vert. Door De Minister Van Vws, Ministerie Van Volksgezondheid, Welzijn En Sport Sposoby zapobiegania agregacji komponentów wirusowych
CN112384615A (zh) 2018-07-04 2021-02-19 普罗拜奥根股份公司 用于纯化包膜病毒的方法
CN111812313B (zh) * 2020-06-22 2021-10-08 北京生物制品研究所有限责任公司 铝佐剂吸附型新型冠状病毒灭活疫苗中抗原的解离方法
CN114544815B (zh) * 2022-03-01 2023-10-27 中牧实业股份有限公司 一种山羊痘病毒的定量检测方法
CN116718524B (zh) * 2023-06-26 2025-11-07 西安交通大学 一种基于细胞膜色谱技术的病毒颗粒检测方法

Citations (4)

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Publication number Priority date Publication date Assignee Title
WO2003093463A1 (en) * 2002-04-30 2003-11-13 Oncolytics Biotech Inc. Improved viral purification methods
CN101098959A (zh) * 2004-10-22 2008-01-02 昂科利蒂克斯生物科技公司 改进的病毒纯化方法
WO2008006780A1 (en) * 2006-07-11 2008-01-17 Bia Separations D.O.O. Method for influenza virus purification
CN100374551C (zh) * 1998-02-17 2008-03-12 先灵公司 含有病毒的组合物以及浓缩病毒制剂的方法

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US5096885A (en) * 1988-04-15 1992-03-17 Genentech, Inc. Human growth hormone formulation
ATE148165T1 (de) * 1989-07-24 1997-02-15 Bayer Ag Stabilisierung von hochgereinigten proteinen
EP0757717B1 (en) * 1994-05-16 2006-05-31 Merck & Co. Inc. Papillomavirus vaccines
CA2437962C (en) 2001-03-16 2005-11-15 Oncolytics Biotech Inc. Method of extracting virus from cell culture
CN101557824B (zh) * 2006-08-07 2012-01-25 财团法人化学及血清疗法研究所 活牛痘疫苗的制备方法
CA2678721C (en) * 2007-03-12 2018-02-13 Oncolytics Biotech Inc. Reoviruses having modified sequences
MX2010005244A (es) * 2007-11-12 2010-10-25 Theraclone Sciences Inc Composiciones y métodos para la terapia y el diagnóstico de influenza.
US8227577B2 (en) * 2007-12-21 2012-07-24 Hoffman-La Roche Inc. Bivalent, bispecific antibodies
MX373096B (es) * 2010-12-02 2020-04-27 Oncolytics Biotech Inc Formulaciones virales líquidas.
EA201390812A1 (ru) * 2010-12-02 2013-11-29 Онколитикс Байотек Инк. Лиофилизированные вирусные составы

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100374551C (zh) * 1998-02-17 2008-03-12 先灵公司 含有病毒的组合物以及浓缩病毒制剂的方法
WO2003093463A1 (en) * 2002-04-30 2003-11-13 Oncolytics Biotech Inc. Improved viral purification methods
CN101098959A (zh) * 2004-10-22 2008-01-02 昂科利蒂克斯生物科技公司 改进的病毒纯化方法
WO2008006780A1 (en) * 2006-07-11 2008-01-17 Bia Separations D.O.O. Method for influenza virus purification

Non-Patent Citations (2)

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Title
Purification of a type-5 recombinant adenovirus encoding human p53 by column chromatography;Bernard G. Huyghe等;《Human Gene Therapy》;19951130;第6卷(第11期);摘要，第1404页左栏第4段-右栏倒数第3段，第1408页左栏第3段 *
柱层析法制备乙型脑炎纯化疫苗的研究;宋宗明等;《微生物学免疫学进展》;20040920;第32卷(第03期);第9-11页 *

Also Published As

Publication number Publication date
KR20140024406A (ko) 2014-02-28
KR102151890B1 (ko) 2020-09-03
JP2014516515A (ja) 2014-07-17
KR20190026059A (ko) 2019-03-12
AU2012248082B2 (en) 2015-08-20
SG194449A1 (en) 2013-12-30
EA201391605A1 (ru) 2014-02-28
AU2012248082A1 (en) 2013-05-09
US20120273424A1 (en) 2012-11-01
EP2702147B1 (en) 2020-07-15
ES2822199T3 (es) 2021-04-29
MX2013012627A (es) 2014-05-13
WO2012145837A1 (en) 2012-11-01
IL228491A (en) 2017-05-29
CN103492557A (zh) 2014-01-01
CA2832367A1 (en) 2012-11-01
TW201307561A (zh) 2013-02-16
IL228491A0 (en) 2013-12-31
JP6029651B2 (ja) 2016-11-24
MX337348B (es) 2016-02-29
EP2702147A4 (en) 2014-11-05
DK2702147T3 (da) 2020-09-28
EP2702147A1 (en) 2014-03-05

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Effective date of registration: 20210508

Address after: Church of Christ, Barbados

Patentee after: Ankolitix Biotechnology (Barbados) Co.

Address before: alberta canada

Patentee before: ONCOLYTICS BIOTECH Inc.

TR01 Transfer of patent right
EE01 Entry into force of recordation of patent licensing contract

Application publication date: 20140101

Assignee: Hangzhou Arnold Biomedical Technology Co.,Ltd.

Assignor: Ankolitix Biotechnology (Barbados) Co.

Contract record no.: X2021990000492

Denomination of invention: Method for purifying virus by gel permeation chromatography

Granted publication date: 20150729

License type: Exclusive License

Record date: 20210816

EE01 Entry into force of recordation of patent licensing contract