MX337348B - Metodos para purificar virus utilizando cromatografia de permeacion en gel. - Google Patents

Metodos para purificar virus utilizando cromatografia de permeacion en gel.

Info

Publication number
MX337348B
MX337348B MX2013012627A MX2013012627A MX337348B MX 337348 B MX337348 B MX 337348B MX 2013012627 A MX2013012627 A MX 2013012627A MX 2013012627 A MX2013012627 A MX 2013012627A MX 337348 B MX337348 B MX 337348B
Authority
MX
Mexico
Prior art keywords
methods
gel permeation
permeation chromatography
purifying viruses
viruses
Prior art date
Application number
MX2013012627A
Other languages
English (en)
Other versions
MX2013012627A (es
Inventor
Matthew C Coffey
Allison Hagerman
Roxna Kapadia
Sarah Serl
Original Assignee
Oncolytics Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Oncolytics Biotech Inc filed Critical Oncolytics Biotech Inc
Publication of MX2013012627A publication Critical patent/MX2013012627A/es
Publication of MX337348B publication Critical patent/MX337348B/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12051—Methods of production or purification of viral material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Medicinal Preparation (AREA)

Abstract

La presente invención se refiere a tampones de elusión y métodos para purificar virus utilizando la cromatografía de permeación en gel. Los métodos son útiles, por ejemplo, para incrementar la recuperación de un virus con una columna de cromatografía de permeación en gel. Los tampones que se usan en los métodos incluyen al menos un excipiente seleccionado de histidina o sacaros, un catión divalente, un detergente no iónico, y una solución salina amortiguada con fosfato.
MX2013012627A 2011-04-29 2012-04-27 Metodos para purificar virus utilizando cromatografia de permeacion en gel. MX337348B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161480561P 2011-04-29 2011-04-29
PCT/CA2012/000406 WO2012145837A1 (en) 2011-04-29 2012-04-27 Methods of purifying viruses using gel permeation chromatography

Publications (2)

Publication Number Publication Date
MX2013012627A MX2013012627A (es) 2014-05-13
MX337348B true MX337348B (es) 2016-02-29

Family

ID=47067091

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013012627A MX337348B (es) 2011-04-29 2012-04-27 Metodos para purificar virus utilizando cromatografia de permeacion en gel.

Country Status (15)

Country Link
US (1) US20120273424A1 (es)
EP (1) EP2702147B1 (es)
JP (1) JP6029651B2 (es)
KR (2) KR102151890B1 (es)
CN (1) CN103492557B (es)
AU (1) AU2012248082B2 (es)
CA (1) CA2832367A1 (es)
DK (1) DK2702147T3 (es)
EA (1) EA201391605A1 (es)
ES (1) ES2822199T3 (es)
IL (1) IL228491A (es)
MX (1) MX337348B (es)
SG (1) SG194449A1 (es)
TW (1) TW201307561A (es)
WO (1) WO2012145837A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EA201390810A1 (ru) 2010-12-02 2013-09-30 Онколитикс Байотек Инк. Жидкие вирусные составы
KR102023207B1 (ko) * 2010-12-02 2019-11-25 온콜리틱스 바이오테크 인코포레이티드 동결건조 바이러스 제형
US10080793B2 (en) 2013-06-17 2018-09-25 De Staat der Nederlanden, vert, door de minister van VWS, Ministerie van Volksgezonheid, Welzijn en Sport Methods for the prevention of aggregation of viral components
US11999975B2 (en) 2018-07-04 2024-06-04 Probiogen Ag Method for purifying an enveloped virus
CN111812313B (zh) * 2020-06-22 2021-10-08 北京生物制品研究所有限责任公司 铝佐剂吸附型新型冠状病毒灭活疫苗中抗原的解离方法
CN114544815B (zh) * 2022-03-01 2023-10-27 中牧实业股份有限公司 一种山羊痘病毒的定量检测方法
CN116718524B (zh) * 2023-06-26 2025-11-07 西安交通大学 一种基于细胞膜色谱技术的病毒颗粒检测方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5096885A (en) * 1988-04-15 1992-03-17 Genentech, Inc. Human growth hormone formulation
DE69029765T2 (de) * 1989-07-24 1997-05-15 Bayer Ag Stabilisierung von hochgereinigten Proteinen
RO117541B1 (ro) * 1994-05-16 2002-04-30 Merck & Co Inc Procedeu pentru prepararea unui vaccin de papillomavirus uman, pentru administrare la om
JP4358434B2 (ja) * 1998-02-17 2009-11-04 シェーリング コーポレイション ウイルスを含む組成物、およびウイルス調製物を濃縮するための方法
DK2253701T3 (da) 2001-03-16 2014-10-20 Oncolytics Biotech Inc Fremgangsmåde til ekstraktion af en virus fra en cellekultur
NZ535944A (en) 2002-04-30 2007-11-30 Oncolytics Biotech Inc Viral purification method comprising a simple extraction step in which a detergent is directly added to the cell culture, thereafter, cell debris is removed from the extraction mixture by filtration or centrifugation
US7901921B2 (en) * 2004-10-22 2011-03-08 Oncolytics Biotech Inc. Viral purification methods
EP1878791A1 (en) * 2006-07-11 2008-01-16 Bia Separations D.O.O. Method for influenza virus purification
US8030020B2 (en) * 2006-08-07 2011-10-04 Juridicial Foundation The Chemo-Sero-Therapeutic Research Institute Process for preparing live smallpox vaccine
KR101647843B1 (ko) * 2007-03-12 2016-08-11 온콜리틱스 바이오테크 인코포레이티드 변형된 서열을 갖는 레오바이러스
AU2008321016B2 (en) * 2007-11-12 2013-03-28 Theraclone Sciences, Inc. Compositions and methods for the therapy and diagnosis of influenza
US8227577B2 (en) * 2007-12-21 2012-07-24 Hoffman-La Roche Inc. Bivalent, bispecific antibodies
EA201390810A1 (ru) * 2010-12-02 2013-09-30 Онколитикс Байотек Инк. Жидкие вирусные составы
KR102023207B1 (ko) * 2010-12-02 2019-11-25 온콜리틱스 바이오테크 인코포레이티드 동결건조 바이러스 제형

Also Published As

Publication number Publication date
DK2702147T3 (da) 2020-09-28
EP2702147A1 (en) 2014-03-05
CN103492557A (zh) 2014-01-01
JP6029651B2 (ja) 2016-11-24
KR102151890B1 (ko) 2020-09-03
EP2702147B1 (en) 2020-07-15
KR20190026059A (ko) 2019-03-12
US20120273424A1 (en) 2012-11-01
CN103492557B (zh) 2015-07-29
AU2012248082A1 (en) 2013-05-09
KR20140024406A (ko) 2014-02-28
IL228491A0 (en) 2013-12-31
TW201307561A (zh) 2013-02-16
CA2832367A1 (en) 2012-11-01
EP2702147A4 (en) 2014-11-05
WO2012145837A1 (en) 2012-11-01
SG194449A1 (en) 2013-12-30
MX2013012627A (es) 2014-05-13
JP2014516515A (ja) 2014-07-17
ES2822199T3 (es) 2021-04-29
IL228491A (en) 2017-05-29
AU2012248082B2 (en) 2015-08-20
EA201391605A1 (ru) 2014-02-28

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