CA2832367A1 - Methods of purifying viruses using gel permeation chromatography - Google Patents
Methods of purifying viruses using gel permeation chromatography Download PDFInfo
- Publication number
- CA2832367A1 CA2832367A1 CA2832367A CA2832367A CA2832367A1 CA 2832367 A1 CA2832367 A1 CA 2832367A1 CA 2832367 A CA2832367 A CA 2832367A CA 2832367 A CA2832367 A CA 2832367A CA 2832367 A1 CA2832367 A1 CA 2832367A1
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- CA
- Canada
- Prior art keywords
- virus
- elution buffer
- gel permeation
- permeation chromatography
- buffered saline
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/424—Elution mode
- B01D15/426—Specific type of solvent
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
- C12N2720/00011—Details
- C12N2720/12011—Reoviridae
- C12N2720/12051—Methods of production or purification of viral material
Landscapes
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicinal Preparation (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
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| PCT/CA2012/000406 WO2012145837A1 (en) | 2011-04-29 | 2012-04-27 | Methods of purifying viruses using gel permeation chromatography |
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| CA2832367A1 true CA2832367A1 (en) | 2012-11-01 |
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| ES2630012T3 (es) | 2010-12-02 | 2017-08-17 | Oncolytics Biotech Inc. | Formulaciones virales liofilizadas |
| EA201390810A1 (ru) * | 2010-12-02 | 2013-09-30 | Онколитикс Байотек Инк. | Жидкие вирусные составы |
| BR112015031541A2 (pt) | 2013-06-17 | 2017-07-25 | De Staat Der Nederlanden Vert Door De Mini Van Vws Miniie Van Volksgezondheid Welzijn En Sport | métodos para o impedimento da agregação de componentes virais |
| CN112384615A (zh) | 2018-07-04 | 2021-02-19 | 普罗拜奥根股份公司 | 用于纯化包膜病毒的方法 |
| CN111812313B (zh) * | 2020-06-22 | 2021-10-08 | 北京生物制品研究所有限责任公司 | 铝佐剂吸附型新型冠状病毒灭活疫苗中抗原的解离方法 |
| CN114544815B (zh) * | 2022-03-01 | 2023-10-27 | 中牧实业股份有限公司 | 一种山羊痘病毒的定量检测方法 |
| CN116718524B (zh) * | 2023-06-26 | 2025-11-07 | 西安交通大学 | 一种基于细胞膜色谱技术的病毒颗粒检测方法 |
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| US5096885A (en) * | 1988-04-15 | 1992-03-17 | Genentech, Inc. | Human growth hormone formulation |
| ATE148165T1 (de) * | 1989-07-24 | 1997-02-15 | Bayer Ag | Stabilisierung von hochgereinigten proteinen |
| BR9507657A (pt) * | 1994-05-16 | 1997-09-09 | Merck & Co Inc | Proteina isolada e purificada de papilomavirus recombinante capsideos particulas semelhantes a virus processos a virus processos para produzir a proteina para tratar ou prevenir a infecçao por papilomavirus para produzir uma proteina de capsideo de papilomavirus recombinante montada em um capsideo ou particula semelhante a virus e para induzir uma resposta imune em um animal vertebrado composiçoes farmacêuticas kit para a detecçao de papilomavirus ou anticorpos para papilomavirus e sistema sinte |
| CA2723040A1 (en) * | 1998-02-17 | 1999-08-19 | Schering Corporation | Compositions comprising viruses and methods for concentrating virus preparations |
| JP4087712B2 (ja) | 2001-03-16 | 2008-05-21 | オンコリティクス バイオテック， インコーポレイティッド | 細胞培養物からウイルスを抽出する方法 |
| ATE427990T1 (de) * | 2002-04-30 | 2009-04-15 | Oncolytics Biotech Inc | Verbesserte reinigungsmethode fur viren |
| US7901921B2 (en) * | 2004-10-22 | 2011-03-08 | Oncolytics Biotech Inc. | Viral purification methods |
| EP1878791A1 (en) * | 2006-07-11 | 2008-01-16 | Bia Separations D.O.O. | Method for influenza virus purification |
| JP5326155B2 (ja) * | 2006-08-07 | 2013-10-30 | 一般財団法人化学及血清療法研究所 | 痘瘡生ワクチンの製造方法 |
| DK2132315T3 (en) * | 2007-03-12 | 2015-10-19 | Oncolytics Biotech Inc | Reoviruses with modified sequences |
| CN102089324B (zh) * | 2007-11-12 | 2014-04-16 | 特罗科隆科学有限公司 | 用于对流行性感冒进行治疗以及诊断的组合物以及方法 |
| US8227577B2 (en) * | 2007-12-21 | 2012-07-24 | Hoffman-La Roche Inc. | Bivalent, bispecific antibodies |
| EA201390810A1 (ru) * | 2010-12-02 | 2013-09-30 | Онколитикс Байотек Инк. | Жидкие вирусные составы |
| ES2630012T3 (es) * | 2010-12-02 | 2017-08-17 | Oncolytics Biotech Inc. | Formulaciones virales liofilizadas |
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Also Published As
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| IL228491A0 (en) | 2013-12-31 |
| JP2014516515A (ja) | 2014-07-17 |
| EP2702147B1 (en) | 2020-07-15 |
| WO2012145837A1 (en) | 2012-11-01 |
| AU2012248082B2 (en) | 2015-08-20 |
| US20120273424A1 (en) | 2012-11-01 |
| CN103492557A (zh) | 2014-01-01 |
| EP2702147A1 (en) | 2014-03-05 |
| KR102151890B1 (ko) | 2020-09-03 |
| JP6029651B2 (ja) | 2016-11-24 |
| MX337348B (es) | 2016-02-29 |
| IL228491A (en) | 2017-05-29 |
| CN103492557B (zh) | 2015-07-29 |
| EP2702147A4 (en) | 2014-11-05 |
| KR20190026059A (ko) | 2019-03-12 |
| SG194449A1 (en) | 2013-12-30 |
| ES2822199T3 (es) | 2021-04-29 |
| EA201391605A1 (ru) | 2014-02-28 |
| DK2702147T3 (da) | 2020-09-28 |
| AU2012248082A1 (en) | 2013-05-09 |
| KR20140024406A (ko) | 2014-02-28 |
| MX2013012627A (es) | 2014-05-13 |
| TW201307561A (zh) | 2013-02-16 |
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| EEER | Examination request |
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| FZDE | Discontinued |
Effective date: 20201007 |