JP6029651B2 - ゲル浸透クロマトグラフィーを使用してウイルスを精製する方法 - Google Patents

ゲル浸透クロマトグラフィーを使用してウイルスを精製する方法 Download PDF

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JP6029651B2
JP6029651B2 JP2014506699A JP2014506699A JP6029651B2 JP 6029651 B2 JP6029651 B2 JP 6029651B2 JP 2014506699 A JP2014506699 A JP 2014506699A JP 2014506699 A JP2014506699 A JP 2014506699A JP 6029651 B2 JP6029651 B2 JP 6029651B2
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virus
elution buffer
gel permeation
buffered saline
permeation chromatography
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JP2014516515A (ja
JP2014516515A5 (enExample
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コフィー，　マシュー　シー．
マシュー シー． コフィー，
アリソン ヘイゲルマン，
アリソン ヘイゲルマン，
ロクソナ カパディア，
ロクソナ カパディア，
サラ セル，
サラ セル，
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オンコリティクス バイオテク，インコーポレーテッド
オンコリティクス バイオテク，インコーポレーテッド
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12051—Methods of production or purification of viral material

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Immunology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicinal Preparation (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
JP2014506699A 2011-04-29 2012-04-27 ゲル浸透クロマトグラフィーを使用してウイルスを精製する方法 Active JP6029651B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161480561P 2011-04-29 2011-04-29
US61/480,561 2011-04-29
PCT/CA2012/000406 WO2012145837A1 (en) 2011-04-29 2012-04-27 Methods of purifying viruses using gel permeation chromatography

Publications (3)

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JP2014516515A JP2014516515A (ja) 2014-07-17
JP2014516515A5 JP2014516515A5 (enExample) 2015-06-18
JP6029651B2 true JP6029651B2 (ja) 2016-11-24

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US (1) US20120273424A1 (enExample)
EP (1) EP2702147B1 (enExample)
JP (1) JP6029651B2 (enExample)
KR (2) KR20140024406A (enExample)
CN (1) CN103492557B (enExample)
AU (1) AU2012248082B2 (enExample)
CA (1) CA2832367A1 (enExample)
DK (1) DK2702147T3 (enExample)
EA (1) EA201391605A1 (enExample)
ES (1) ES2822199T3 (enExample)
IL (1) IL228491A (enExample)
MX (1) MX337348B (enExample)
SG (1) SG194449A1 (enExample)
TW (1) TW201307561A (enExample)
WO (1) WO2012145837A1 (enExample)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2630012T3 (es) 2010-12-02 2017-08-17 Oncolytics Biotech Inc. Formulaciones virales liofilizadas
EA201390810A1 (ru) * 2010-12-02 2013-09-30 Онколитикс Байотек Инк. Жидкие вирусные составы
BR112015031541A2 (pt) 2013-06-17 2017-07-25 De Staat Der Nederlanden Vert Door De Mini Van Vws Miniie Van Volksgezondheid Welzijn En Sport métodos para o impedimento da agregação de componentes virais
CN112384615A (zh) 2018-07-04 2021-02-19 普罗拜奥根股份公司 用于纯化包膜病毒的方法
CN111812313B (zh) * 2020-06-22 2021-10-08 北京生物制品研究所有限责任公司 铝佐剂吸附型新型冠状病毒灭活疫苗中抗原的解离方法
CN114544815B (zh) * 2022-03-01 2023-10-27 中牧实业股份有限公司 一种山羊痘病毒的定量检测方法
CN116718524B (zh) * 2023-06-26 2025-11-07 西安交通大学 一种基于细胞膜色谱技术的病毒颗粒检测方法

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US5096885A (en) * 1988-04-15 1992-03-17 Genentech, Inc. Human growth hormone formulation
ATE148165T1 (de) * 1989-07-24 1997-02-15 Bayer Ag Stabilisierung von hochgereinigten proteinen
BR9507657A (pt) * 1994-05-16 1997-09-09 Merck & Co Inc Proteina isolada e purificada de papilomavirus recombinante capsideos particulas semelhantes a virus processos a virus processos para produzir a proteina para tratar ou prevenir a infecçao por papilomavirus para produzir uma proteina de capsideo de papilomavirus recombinante montada em um capsideo ou particula semelhante a virus e para induzir uma resposta imune em um animal vertebrado composiçoes farmacêuticas kit para a detecçao de papilomavirus ou anticorpos para papilomavirus e sistema sinte
CA2723040A1 (en) * 1998-02-17 1999-08-19 Schering Corporation Compositions comprising viruses and methods for concentrating virus preparations
JP4087712B2 (ja) 2001-03-16 2008-05-21 オンコリティクス バイオテック， インコーポレイティッド 細胞培養物からウイルスを抽出する方法
ATE427990T1 (de) * 2002-04-30 2009-04-15 Oncolytics Biotech Inc Verbesserte reinigungsmethode fur viren
US7901921B2 (en) * 2004-10-22 2011-03-08 Oncolytics Biotech Inc. Viral purification methods
EP1878791A1 (en) * 2006-07-11 2008-01-16 Bia Separations D.O.O. Method for influenza virus purification
JP5326155B2 (ja) * 2006-08-07 2013-10-30 一般財団法人化学及血清療法研究所 痘瘡生ワクチンの製造方法
DK2132315T3 (en) * 2007-03-12 2015-10-19 Oncolytics Biotech Inc Reoviruses with modified sequences
CN102089324B (zh) * 2007-11-12 2014-04-16 特罗科隆科学有限公司 用于对流行性感冒进行治疗以及诊断的组合物以及方法
US8227577B2 (en) * 2007-12-21 2012-07-24 Hoffman-La Roche Inc. Bivalent, bispecific antibodies
EA201390810A1 (ru) * 2010-12-02 2013-09-30 Онколитикс Байотек Инк. Жидкие вирусные составы
ES2630012T3 (es) * 2010-12-02 2017-08-17 Oncolytics Biotech Inc. Formulaciones virales liofilizadas

Also Published As

Publication number Publication date
IL228491A0 (en) 2013-12-31
JP2014516515A (ja) 2014-07-17
CA2832367A1 (en) 2012-11-01
EP2702147B1 (en) 2020-07-15
WO2012145837A1 (en) 2012-11-01
AU2012248082B2 (en) 2015-08-20
US20120273424A1 (en) 2012-11-01
CN103492557A (zh) 2014-01-01
EP2702147A1 (en) 2014-03-05
KR102151890B1 (ko) 2020-09-03
MX337348B (es) 2016-02-29
IL228491A (en) 2017-05-29
CN103492557B (zh) 2015-07-29
EP2702147A4 (en) 2014-11-05
KR20190026059A (ko) 2019-03-12
SG194449A1 (en) 2013-12-30
ES2822199T3 (es) 2021-04-29
EA201391605A1 (ru) 2014-02-28
DK2702147T3 (da) 2020-09-28
AU2012248082A1 (en) 2013-05-09
KR20140024406A (ko) 2014-02-28
MX2013012627A (es) 2014-05-13
TW201307561A (zh) 2013-02-16

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