TR200002909T2 - Rekombinant potivirüs yapısı ve kullanımı - Google Patents

Rekombinant potivirüs yapısı ve kullanımı

Info

Publication number
TR200002909T2
TR200002909T2 TR2000/02909T TR200002909T TR200002909T2 TR 200002909 T2 TR200002909 T2 TR 200002909T2 TR 2000/02909 T TR2000/02909 T TR 2000/02909T TR 200002909 T TR200002909 T TR 200002909T TR 200002909 T2 TR200002909 T2 TR 200002909T2
Authority
TR
Turkey
Prior art keywords
potivirus
construct
recombinant
substitution
plants
Prior art date
Application number
TR2000/02909T
Other languages
English (en)
Inventor
Gal-On Amit
Original Assignee
State Of Israel/Ministry Of Agriculture;
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by State Of Israel/Ministry Of Agriculture; filed Critical State Of Israel/Ministry Of Agriculture;
Publication of TR200002909T2 publication Critical patent/TR200002909T2/tr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/60—Isolated nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C12N9/506—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses derived from RNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/34011—Potyviridae
    • C12N2770/34022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Mevcut bulus; bitki çapraz korumasi için faydali olan, rekombinant potivirüs bulastirici bir nükleik asit, tercihen cDNA veya RNA yapisi ile ilgili olup, bahsedilen yapi; HC-Pro geni korunan FRNK kutusu dizisinin, bir substitüsyonu, tercihen Arg'nin bir substitüsyonunu içermesiyle karakterize edilen tam uzunlukta bir klondan olusmaktadir. Tercihen, bahsedilen yapi; ayrica, afid transmissibilitesini etkili olarak durduran bir substitüsyon içermektedir. Bunun yaninda, mevcut bulus; yukarida bahsedilen yapidan faydalanarak, potivirüsün ilimli bir türünün üretimi için bir metot ve viral enfeksiyondan bitkileri korumak için ve bahsedilen yapiyi kullanarak, bitkilerde, yabanci nükleik asit (genlerinin) kisa süreli verilmesi için bir metot ile ilgilidir. Tercihen mevcut bulus; ZYMV enfeksiyonuna karsi, curcurbitlerin çapraz korunmasi için bir metot ile ilgilidir. Bundan baska, mevcut bulus; bitkileri, mekanik asilama veya bombardiman yoluyla, rekombinant potivirüs ..(devami var)
TR2000/02909T 1998-04-07 1999-03-30 Rekombinant potivirüs yapısı ve kullanımı TR200002909T2 (tr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IL12399498 1998-04-07

Publications (1)

Publication Number Publication Date
TR200002909T2 true TR200002909T2 (tr) 2001-02-21

Family

ID=11071404

Family Applications (1)

Application Number Title Priority Date Filing Date
TR2000/02909T TR200002909T2 (tr) 1998-04-07 1999-03-30 Rekombinant potivirüs yapısı ve kullanımı

Country Status (7)

Country Link
EP (1) EP1068330A2 (tr)
JP (1) JP2002536956A (tr)
CN (1) CN1303433A (tr)
AU (1) AU3052599A (tr)
CA (1) CA2327445A1 (tr)
TR (1) TR200002909T2 (tr)
WO (1) WO1999051749A2 (tr)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU1602301A (en) 1999-11-12 2001-06-06 University Of South Carolina Method for enhancing resistance in plants
CN1575336A (zh) * 2000-11-28 2005-02-02 病毒基因有限公司 给受试者送递干扰素的系统和方法
IL151882A0 (en) * 2001-01-24 2003-04-10 Virogene Ltd Vectors capable of imparting herbicide resistance and viral cross protection
JP2002238574A (ja) * 2001-02-16 2002-08-27 Japan International Research Center For Agricultural Services パパイア奇形葉モザイクウイルスの全長ゲノムｒｎａ
KR101103705B1 (ko) * 2007-12-26 2012-01-11 서울여자대학교 산학협력단 고감염성 고추모틀바이러스 핵산분자 및 이로부터 유래된 식물 바이러스 벡터
CN102277376B (zh) * 2010-06-10 2013-09-25 叶锡东 提供作物广谱病毒抗性的包含木瓜环斑病毒辅助组分蛋白酶基因的基因转移载体及其应用
EP2514304A1 (en) 2011-04-20 2012-10-24 Syngenta Participations AG Cucurbita plant resistant to potyvirus
CN102379305B (zh) * 2011-09-28 2013-11-20 中国农业大学 玉米铁氧还蛋白的新用途
CN102796739B (zh) * 2012-08-16 2014-02-19 北京农业生物技术研究中心 TuMV-CP基因片段介导的RNAi载体在培育抗TuMV转基因植物中的应用
CN104531756A (zh) * 2014-10-15 2015-04-22 湖南省烟草公司长沙市公司 一种使烟草获得马铃薯y病毒抗性的方法及vigs载体
CN107964551A (zh) * 2017-12-07 2018-04-27 山东农业大学 西瓜花叶病毒侵染性克隆表达载体的构建与应用
CN108823174B (zh) * 2018-06-28 2021-07-20 山东农业大学 番木瓜环斑病毒西瓜株系弱毒疫苗的研制及其应用
CN111118216B (zh) * 2020-01-18 2022-09-20 广西壮族自治区农业科学院 检测甜椒叶脉斑驳病毒的pcr引物组、试剂盒及其应用
CN116675751B (zh) * 2023-06-08 2024-01-26 山东农业大学 SWEET1g蛋白及其编码基因在抗马铃薯病毒中的应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5491076A (en) * 1993-11-01 1996-02-13 The Texas A&M University System Expression of foreign genes using a replicating polyprotein producing virus vector

Also Published As

Publication number Publication date
EP1068330A2 (en) 2001-01-17
WO1999051749A3 (en) 1999-12-09
WO1999051749A2 (en) 1999-10-14
JP2002536956A (ja) 2002-11-05
AU3052599A (en) 1999-10-25
CN1303433A (zh) 2001-07-11
CA2327445A1 (en) 1999-10-14

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