TR199701010T1 - Safra tuzu stimülasyonlu lipazin ekspresyonu için dna. - Google Patents

Safra tuzu stimülasyonlu lipazin ekspresyonu için dna.

Info

Publication number
TR199701010T1
TR199701010T1 TR97/01010T TR9701010T TR199701010T1 TR 199701010 T1 TR199701010 T1 TR 199701010T1 TR 97/01010 T TR97/01010 T TR 97/01010T TR 9701010 T TR9701010 T TR 9701010T TR 199701010 T1 TR199701010 T1 TR 199701010T1
Authority
TR
Turkey
Prior art keywords
polypeptide
pichia pastoris
expression
dna
bile salt
Prior art date
Application number
TR97/01010T
Other languages
English (en)
Inventor
Das Goutam
Original Assignee
Astra Aktiebolag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=20398427&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=TR199701010(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Astra Aktiebolag filed Critical Astra Aktiebolag
Publication of TR199701010T1 publication Critical patent/TR199701010T1/tr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Bu bulus metilotrofik mantar pichia pastoris' de safra tuzu stimülasyonlu lipazin (BSSL) ekspresyonuna yönelik metodlarla kullanilmak üzere DNA molekülleri, rekombinant vektörler ve hücre kültürleriyle ilgilidir. DNA molekülü sunlari ihtiva eder: (a) bir insan BSSL'si veya bunun biyolojik yönden aktif bir varyanti olan bir polipeptidi kodlayan bir bölge; (b) sözü geçen polipeptid kodlayici bölgenin 5'- ucuna bagli bir vaziyette, sözü geçen DNA molekülü ile transforme edilmis pichia pastoris hücrelerinden sözü geçen polipeptidin sekresiyonunu yönlendirme kabiliyetine sahip olan bir sinyal peptidini kodlayan bir bölge; ve (c) islev yapabilecek bir vaziyette (a) ve (b)' de tayin edilen sözü geçen kodlayici bölgelere bagli olan pichia pastoris' in metanol oksidaz promoteri veya fonksiyonel olarak esdeger bir promoter.
TR97/01010T 1995-05-24 1996-03-12 Safra tuzu stimülasyonlu lipazin ekspresyonu için dna. TR199701010T1 (tr)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
SE9501939A SE9501939D0 (sv) 1995-05-24 1995-05-24 DNA molecules for expression of polypeptides

Publications (1)

Publication Number Publication Date
TR199701010T1 true TR199701010T1 (tr) 1998-01-21

Family

ID=20398427

Family Applications (1)

Application Number Title Priority Date Filing Date
TR97/01010T TR199701010T1 (tr) 1995-05-24 1996-03-12 Safra tuzu stimülasyonlu lipazin ekspresyonu için dna.

Country Status (15)

Country Link
EP (1) EP0832257A1 (tr)
JP (1) JP2000510683A (tr)
KR (1) KR19980703238A (tr)
CN (1) CN1185812A (tr)
AU (1) AU5165696A (tr)
CZ (1) CZ297397A3 (tr)
EE (1) EE9700321A (tr)
HU (1) HUP9802388A3 (tr)
IL (1) IL118335A0 (tr)
PL (1) PL322848A1 (tr)
RU (1) RU2157847C2 (tr)
SE (1) SE9501939D0 (tr)
TR (1) TR199701010T1 (tr)
TW (1) TW434314B (tr)
WO (1) WO1996037622A1 (tr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2001255501A1 (en) * 2000-04-21 2001-11-07 Monsanto Technology Llc Blood-pressure reducing polypeptides containing vpp derived from microorganisms
KR100717353B1 (ko) * 2006-04-12 2007-05-11 한국생명공학연구원 피키아 파스토리스 유래의 자동 유도성 ｎｐｓ 프로모터 및이를 이용한 이종 단백질의 제조방법
CN103952386A (zh) * 2014-03-31 2014-07-30 四川农业大学 利用毕赤酵母高效分泌表达重组猪胰腺脂肪酶ppl的方法
RU2697218C1 (ru) * 2018-07-11 2019-08-13 федеральное государственное автономное образовательное учреждение высшего образования "Московский физико-технический институт (национальный исследовательский университет)" Использование сигнальных пептидов митохондриальной локализации для увеличения уровня гетерологической экспрессии белков в P.pastoris и S.cerevisiae
EP3999115A4 (en) * 2019-07-12 2023-11-22 LipUm AB NEW BSSL ANTIBODIES

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69133066T2 (de) * 1990-01-16 2003-03-06 Ct Ingenieria Genetica Biotech Verfahren zur Expression heterologischer Gene in der Hefe Pichia Pastoris, Expressionsverfahren und transformierte Mikroorganismen
IS4130A (is) * 1993-03-01 1994-09-02 Ab Astra Ný fjölpeptíð

Also Published As

Publication number Publication date
RU2157847C2 (ru) 2000-10-20
SE9501939D0 (sv) 1995-05-24
IL118335A0 (en) 1996-09-12
HUP9802388A2 (hu) 1999-02-01
AU5165696A (en) 1996-12-11
TW434314B (en) 2001-05-16
CZ297397A3 (cs) 1998-03-18
EP0832257A1 (en) 1998-04-01
JP2000510683A (ja) 2000-08-22
HUP9802388A3 (en) 2000-10-30
PL322848A1 (en) 1998-02-16
KR19980703238A (ko) 1998-10-15
WO1996037622A1 (en) 1996-11-28
EE9700321A (et) 1998-06-15
CN1185812A (zh) 1998-06-24

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