HUP9802388A2 - DNS-molekula epesó-stimulált lipáz (BSSL) kifejezésére - Google Patents
DNS-molekula epesó-stimulált lipáz (BSSL) kifejezéséreInfo
- Publication number
- HUP9802388A2 HUP9802388A2 HU9802388A HUP9802388A HUP9802388A2 HU P9802388 A2 HUP9802388 A2 HU P9802388A2 HU 9802388 A HU9802388 A HU 9802388A HU P9802388 A HUP9802388 A HU P9802388A HU P9802388 A2 HUP9802388 A2 HU P9802388A2
- Authority
- HU
- Hungary
- Prior art keywords
- bssl
- expression
- enzyme
- bile salt
- stimulated lipase
- Prior art date
Links
- 102100035687 Bile salt-activated lipase Human genes 0.000 title abstract 6
- 108010087173 bile salt-stimulated lipase Proteins 0.000 title abstract 6
- 108020004414 DNA Proteins 0.000 title abstract 3
- 102000053602 DNA Human genes 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 239000013604 expression vector Substances 0.000 abstract 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 abstract 2
- 108091026890 Coding region Proteins 0.000 abstract 1
- 102000004316 Oxidoreductases Human genes 0.000 abstract 1
- 108090000854 Oxidoreductases Proteins 0.000 abstract 1
- 241000235648 Pichia Species 0.000 abstract 1
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 1
- 108010051210 beta-Fructofuranosidase Proteins 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 abstract 1
- 230000029087 digestion Effects 0.000 abstract 1
- 235000013350 formula milk Nutrition 0.000 abstract 1
- 239000001573 invertase Substances 0.000 abstract 1
- 235000011073 invertase Nutrition 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
- 210000005253 yeast cell Anatomy 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A találmány tárgyát epesó-stiműlált lipáz (BSSL) enzim transzfőrmáltmikrőőrganizműsőkban való kifejezésére szőlgáló eljárás, a BSSL-t vagybiőlógiailag aktív váltőzatát kódőló DNS-mőlekűlák, és ezekettartalmazó vektőrők, valamint a BSSL kifejezésére alkalmas expressziósvektőrral transzfőrmált sejtek képezik. A BSSL termelését a kódőló szekvencián kívül egy metanől őxidázprőmőtert és Saccharőmyces cerevisiae eredetű invertáz szignálpeptidszekvenciákat kódőló szakaszőkat tartalmazó DNS-t magába főglalóexpressziós vektőrral transzfőrmált Pichia pastőralis metilőtrófélesztősejttel végzik. A termelt enzimet előnyösen csecsemőtápszerekben zsíremésztéstelősegítő alkőtóként alkalmazzák. ŕ
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
SE9501939A SE9501939D0 (sv) | 1995-05-24 | 1995-05-24 | DNA molecules for expression of polypeptides |
Publications (2)
Publication Number | Publication Date |
---|---|
HUP9802388A2 true HUP9802388A2 (hu) | 1999-02-01 |
HUP9802388A3 HUP9802388A3 (en) | 2000-10-30 |
Family
ID=20398427
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HU9802388A HUP9802388A3 (en) | 1995-05-24 | 1996-03-12 | A dna molecule for expression of bile salt-stimulated lipase (bssl) |
Country Status (15)
Country | Link |
---|---|
EP (1) | EP0832257A1 (hu) |
JP (1) | JP2000510683A (hu) |
KR (1) | KR19980703238A (hu) |
CN (1) | CN1185812A (hu) |
AU (1) | AU5165696A (hu) |
CZ (1) | CZ297397A3 (hu) |
EE (1) | EE9700321A (hu) |
HU (1) | HUP9802388A3 (hu) |
IL (1) | IL118335A0 (hu) |
PL (1) | PL322848A1 (hu) |
RU (1) | RU2157847C2 (hu) |
SE (1) | SE9501939D0 (hu) |
TR (1) | TR199701010T1 (hu) |
TW (1) | TW434314B (hu) |
WO (1) | WO1996037622A1 (hu) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2001255501A1 (en) * | 2000-04-21 | 2001-11-07 | Monsanto Technology Llc | Blood-pressure reducing polypeptides containing vpp derived from microorganisms |
KR100717353B1 (ko) * | 2006-04-12 | 2007-05-11 | 한국생명공학연구원 | 피키아 파스토리스 유래의 자동 유도성 nps 프로모터 및이를 이용한 이종 단백질의 제조방법 |
CN103952386A (zh) * | 2014-03-31 | 2014-07-30 | 四川农业大学 | 利用毕赤酵母高效分泌表达重组猪胰腺脂肪酶ppl的方法 |
RU2697218C1 (ru) * | 2018-07-11 | 2019-08-13 | федеральное государственное автономное образовательное учреждение высшего образования "Московский физико-технический институт (национальный исследовательский университет)" | Использование сигнальных пептидов митохондриальной локализации для увеличения уровня гетерологической экспрессии белков в P.pastoris и S.cerevisiae |
BR112022000391A2 (pt) * | 2019-07-12 | 2022-03-03 | Lipum Ab | Novos anticorpos bssl |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ATE220721T1 (de) * | 1990-01-16 | 2002-08-15 | Ct Ingenieria Genetica Biotech | Verfahren zur expression heterologischer gene in der hefe pichia pastoris, expressionsvektoren und transformierte mikroorganismen |
IS4130A (is) * | 1993-03-01 | 1994-09-02 | Ab Astra | Ný fjölpeptíð |
-
1995
- 1995-05-24 SE SE9501939A patent/SE9501939D0/xx unknown
-
1996
- 1996-03-12 EP EP96908415A patent/EP0832257A1/en not_active Withdrawn
- 1996-03-12 KR KR1019970706642A patent/KR19980703238A/ko not_active Application Discontinuation
- 1996-03-12 EE EE9700321A patent/EE9700321A/xx unknown
- 1996-03-12 CN CN96194083A patent/CN1185812A/zh active Pending
- 1996-03-12 CZ CZ972973A patent/CZ297397A3/cs unknown
- 1996-03-12 AU AU51656/96A patent/AU5165696A/en not_active Abandoned
- 1996-03-12 WO PCT/SE1996/000318 patent/WO1996037622A1/en not_active Application Discontinuation
- 1996-03-12 TR TR97/01010T patent/TR199701010T1/xx unknown
- 1996-03-12 RU RU97117367/13A patent/RU2157847C2/ru not_active IP Right Cessation
- 1996-03-12 PL PL96322848A patent/PL322848A1/xx unknown
- 1996-03-12 JP JP08535592A patent/JP2000510683A/ja not_active Abandoned
- 1996-03-12 HU HU9802388A patent/HUP9802388A3/hu unknown
- 1996-04-10 TW TW085104253A patent/TW434314B/zh active
- 1996-05-20 IL IL11833596A patent/IL118335A0/xx unknown
Also Published As
Publication number | Publication date |
---|---|
AU5165696A (en) | 1996-12-11 |
SE9501939D0 (sv) | 1995-05-24 |
PL322848A1 (en) | 1998-02-16 |
WO1996037622A1 (en) | 1996-11-28 |
RU2157847C2 (ru) | 2000-10-20 |
EP0832257A1 (en) | 1998-04-01 |
IL118335A0 (en) | 1996-09-12 |
JP2000510683A (ja) | 2000-08-22 |
TW434314B (en) | 2001-05-16 |
CN1185812A (zh) | 1998-06-24 |
CZ297397A3 (cs) | 1998-03-18 |
HUP9802388A3 (en) | 2000-10-30 |
KR19980703238A (ko) | 1998-10-15 |
EE9700321A (et) | 1998-06-15 |
TR199701010T1 (xx) | 1998-01-21 |
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