SU1252713A1 - Method of quantitative determining of pentamine - Google Patents

Abstract

This invention relates to analytical chemistry. The purpose of the invention is to improve the accuracy and reduce the time of determination by replacing the chemical reagent with a mixture of cobalt sulfate and ammonium rohanide at a certain ratio of components. A precise sample of pentamine is placed in a 25 ml volumetric flask. The flask is poured water to the mark. 3 ml of the solution obtained, 1 ml of a 0.2 M solution of cobalt sulfate, and 1 ml of a 4 M solution of ammonium rodanide are added to a separatory funnel. The resulting mixture was extracted for 1 minute and then its optical density was measured. 2 tab. . § (L o tn

Description

This invention relates to an analytical X1H (and, in particular, to extraction-on-photometric determination of pentamine.

The purpose of the invention is to increase accuracy and reduce the time of determination.

The goal is achieved by replacing the x} tmic reagent with a mixture of cobalt sulfate and ammonium rohanide at a certain ratio of components.

Example 1. A precise weight (about 0.02 g) of pentamine is placed in a 25 ml volumetric flask, the contents of the flask are dissolved in water and adjusted to the mark with water. 3 ml of the resulting solution, 1 ml 0.2 M are added to a separatory funnel. cobalt sulfate solution and 1 ml of 4M ammonium rohanide solution. The resulting mixture was extracted for 1 min., After 1 min, the optical density was measured using a FEK-56M photoelectrocolorimeter with a red light filter in a cuvette with a layer thickness of 1 cm.

The proposed method allows the quantitative determination of pentamine to be within 780-7800 µg / 5 ml.

Example 2. Determination of pentamine in the solution for injection of 5% - 1 ml.

1 MP of 5% pentamine solution is placed in a 100 ml volumetric flask, the contents of the flask are dissolved.

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In parallel, the measurement of the optical density of the dichloroethane extract of the standard pentamine solution was carried out.

The content of pentamine in percentage (x) is calculated by the formula

L, 0.002 100% V

Yes

V,

where a is the sample of the test substance, rj

optical density of the studied extract; optical density of the extract using a standard solution;

d, d

V v

- the multiplicity of breeding;

0.002 is the content of pentamine in 1 ml of the standard solution (the standard solution of pentamine is prepared by dissolving a preparation that meets the requirements of the State Pharmacopoeia, X ed.).

The results of the quantitative determination of pentamine are shown in Table 1.

Table 1

in water and adjusted to the mark with water. 3 ml of the solution obtained is introduced into a separatory funnel and then proceeds as in Example 1.

The results of the quantitative determination of pentamine in solution for

injections of 5% - 1.0 in table.2.

ml are given

table 2

Relative method error 0.71%.

Claims (1)

Invention Formula A method for quantitative determination of pentamine by sequential processing of an analyzed sample with a chemical reagent and an organic solvent followed by separation and photometry of the organic layer, characterized in that, in order to improve accuracy and reduce the time of determination, a mixture of cobalt sulfate and ammonium rohanide is used at the next mole The relative ratio of components, respectively: (0.16-3.2): {0.3-7.2).