SU1665287A1 - Method of determination of aminazine in a compound preparation - Google Patents

Abstract

The invention relates to analytical chemistry, in particular the quantitative determination of aminazine [2-chloro-10- (3-dimethylaminopropyl) phenothiazine hydrochloride]. The goal is to increase the selectivity of the analysis. For this, the sample is dissolved in water, stirred and filtered, the aliquot is treated with 6 to 20% sodium hydroxide solution, the precipitate is separated and dissolved in ethyl acetate. Aminazin reextract 0.1 n. With the solution of hydrochloric acid, the analyzed reextract is added to the standard solution and treated with a 0.3–1.0% vol. solution of ferric chloride, followed by photometry of the colored solution. Error of determination is ± 3.0 rel.%. 3 tab.

Description

The invention relates to analytical chemistry, and specifically to methods for the quantitative determination of aminazine (2-chloro-10- (3-dimethylaminopropyl) -pheno-azine hydrochloride)

The purpose of the invention is to increase the selectivity of the method

PRI me R 1. The weight of the complex drug aminavita about 2.3 composition: vitamin A 9.1 ± 1.8 mg, vitamin D2 1.0 ± 0.2 mg, vitamin E 20.0 ± 4.0. Vitamin 82 10.0 ± 1.0mg. Vitamin B1 30.5 + 3.0 mg. vitamin C 238.1 ± 23.8 mg, aminazin 50.0 ± 5.0 mg. tetracycline 588.6 + 58.9 mg, flour - up to 2.3 g is weighed with an accuracy of at least 0.0002 g, dissolved in distilled water in a 100 ml volumetric flask, the volume of the solution is brought to the mark with water, mixed and filtered through filter paper . five

The ml of filtrate is transferred to a 100 ml separatory funnel. 2 ml of 10% sodium hydroxide solution are added. The precipitate is extracted with 2 times 15 ml of ethyl acetate

The ethyl acetate solution is transferred to a separatory funnel and reextrated 3 times with 30 ml of 0.1 N. hydrochloric acid solution. The reextract is poured into a 100 ml volumetric flask, adjusted to the 0.1 n mark. solution of hydrochloric acid and mix.

Similarly, come with a standard solution aminazina.

In a flask No. 1 and No. 2 place on 4 ml of the analyzed solution. In a flask No. 1 ml of standard solution is poured, in a flask of isfe 2 - 2 ml 0.1 n. hydrochloric acid solution, in flask number 3 - 6 ml 0.1 n. hydrochloric acid solution. 10 ml are poured into all flasks.

Oo ate yu

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0.5% ferric chloride solution. And then the optical density is measured on a spectrophotometer at a wavelength of 525 nm in a cell with a layer thickness of 10 mm, using the solution in flask No. 3 as a control solution.

Processing results

The content of aminazine X in one dose of the drug in mg is credited according to the formula

5-100-2-a Dr25-a

(D-Di) -4-m (D-Di) -m

Di-optical density of the test solution;

D-Di is the optical density of the standard solution;

D is the total optical density of the test and standard solution;

100.4.2 - dilution, ml;

m is the mass of the sample of the drug, g;

0.5 - aminazine content in 1 ml of standard solution, mg;

a is the dose of the drug, g, and 51.3 mg of aminazine are found.

A standard sample is prepared as follows.

0.05 g (exact weight) of aminazine is dissolved in a 100 ml volumetric flask, the solution is made up to the mark with water, stirred with 1 ml of the standard solution of the standard sample contains 0.5 mg of aminazine.

Table 1 shows the results of the determination of aminazine in aminavit depending on the volume concentration of ferric chloride.

As can be seen from Table 1, the optimal concentration of ferric chloride is 0.3-1.0% by volume.

Table 2 shows the results of the determination of aminazine in aminavit depending on the volume concentration of sodium hydroxide.

As can be seen from Table 2, the optimum concentration of sodium hydroxide is 6-20% by volume.

In tab. Figure 3 shows the results of the determination of aminazine in aminavite, depending on the volume of added 10% sodium hydroxide,

As can be seen from Table 3, the optimal volume of 10% sodium hydroxide is 1.5-5.0 ml.

The described method makes it possible to selectively determine aminazine in the aminovite with the relative error Z.O.

Claims (1)

The method of determining chlorpromazine in a complex preparation, including treating with chlorine iron and photometry of the solution obtained, characterized in that, in order to increase the selectivity of the determination, the sample to be analyzed is dissolved in water, treated with sodium hydroxide solution, the precipitate is separated, dissolved in ethyl acetate, treated with 0.1 n. hydrochloric acid and ferric chloride solution. Table 1 table 2