SU1343356A1 - Method of determining vitamin "i" in biological materials - Google Patents

Description

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The invention relates to a technique for quantifying the content of vitamin U (methylmethionine sulphony, S-methylmethionine, MMS), which is widely used in product quality control in the feed industry.

The purpose of the invention is to increase the efficiency of the method.

Example. A portion of the 2 g premix was placed in a 100 cm volumetric flask, 60 cm of water was poured in and shaken on a rocking chair for 5 minutes. Then the contents of the flask are brought to the mark with water, mixed and filtered,

Take 5-10 cm of filta-rata in a 25-cm volumetric flask, add 2.5 cm of 1 N hydrochloric acid solution and 5 cm of a 2% aqueous solution of ammonium reinecate, mix and leave for 2 hours in the refrigerator. After deposition of the precipitate, the solution is filtered on a shot of Shott No. 2 into a Bunsen flask using a vacuum pump. The crystals that remained on the filter were washed three times with 5 cm portions of cooled 0.1 N hydrochloric acid. The Bunsen flask was changed, the precipitate was dissolved in 70% ethyl alcohol solution, the tide was taken in three doses of 5 cm, each time carefully stirring the layer with the precipitate.

The obtained filtrate is quantitatively transferred to a measuring tube with a capacity of 15 cm, brought up to the mark with ethanol solution, mixed and the optical density is measured on a spectrophotometer at a wavelength of 520 nm in cuvettes with a 10 mm thick light absorbing layer relative to a 0% ethanol solution.

The calculation of the content of vitamin U (x) is carried out according to a calibration graph, built on a standard solution with a concentration of vitamin 1 mg / cm using the formula

343356

(kg / t)

K V

-Y

P

t 10 fc

five

10 where K is the mass of the vitamin found on the calibration schedule, mg: V is the volume of the aqueous extract,

cm ; 0 V is the volume of filtrate taken

for analysis,

m is the mass of the premix weight, g; conversion factors of milligrams to kilograms (numerator) and 5 grams to tons (denominator),

The results of testing the proposed and well-known methods for determining vitamin U are given in Table 1 and 2 (vitamin U was added to 0 premix 30.0 kg / ton).

Analysis of the experimental data shows that the vitamin content in the premixes, as determined by the proposed method, with a concentration of ammonium reinecat ammonium solution of 2.0 - 2.5%, is 29.2 + 0.6 - 30.01 +0 , 5% kg / t, and at its concentration of 1.0-1.5% is 15.5 ± 0.5 -23, 5 to, 6 kg / t; when determining 0 in a known manner - 25, Oil, O kg / t

with deviations down to 2.4–7.5 kg / t in relation to the amount injected (Table 1).

Due to the limited solubility of ammonium reinecat in water, its concentration of more than 2.5% has not been tested.

The most optimal concentration of ethanol is 50-75%, at which the hardly soluble set of vitamin-di-dienecate 0 dissolves quickly. In this case, the content of vitamin U was 29.310.3 -29, 71 0.6 kg / t, and ethanol concentrations of 40 and 96%, the amount of vitamins was 26.4iO, 6 and 25.9 ± 0.4 kg / t, t, e, respectively, was underestimated (Table 2). Vitamin U was determined 16% less by a known method compared to the entered.

The content of vitamin U, kg / t, according to the method

31,627,6

28,222,5

29,224,7

29,126,7

28,

Total: 15.5 tO, 5 23.5 ± 0.6 29.5 ± 0.4 30.0 ± 0.5 29.2 tO, 6 25.0 tl, 0

Total: 26.410.6 29.6iO, 5 29.4iO, 5 29.3tO, 3 29.7 + 0.6 25.910.4 25.2il, 0

Compiled by I.Tareyev Editor V. Danko Tehred..Dvdyk

Order 4818/46 Circulation 776 Subscription

VNIIPI USSR State Committee

for inventions and discoveries 113035, Moscow, Zh-35, Raushsk nab., 4/5

Production and printing company, Uzhgorod, Projecto st., 4

table 2

Proofreader L. Patay

Claims (1)

METHOD FOR DETERMINING VITAMIN U IN BIOLOGICAL MATERIALS, including extraction, precipitation of vitamin U with a solution of ammonium reinecate, followed by colorimetry of substances formed during processing, characterized in that, in order to increase the efficiency of the method, the extraction is carried out for 1 N. hydrochloric acid solution, after precipitation, the resulting crystals are washed with 0.1 N. hydrochloric acid solution and dissolved in a 50-70% ethanol solution, and the resulting crystal solution was subjected to colorimetry. (L with so with SP Ci I 343356