SU1255902A1 - Method of quantitative determining of quinine hydrochloride - Google Patents

Abstract

This invention relates to analytical chemistry, in particular to a method for the determination of quinine hydrochloride, a drug. The purpose of the image is the retention — the increase in the detection sensitivity. The last suspension is the treatment of 1 ml of 1-10 M solution of quinine hydrochloride with 1 ml of sodium salicylate solution, 1 ml of I lO M iron nitrate solution, 0.4 ml of 1N. Acetic acid solution, you. The reaction product is extracted with 5 ml of chloroform, the extract is separated and photometrized. The sensitivity of the method is 0.01-0.025 mg / ml. 6 tab. a 3

Description

solution of acetic acid M quinine solution

This invention relates to analytical chemistry, in particular to methods for the extraction-photometric determination of quinine hydrochloride in a preparation and in dosage forms.

The purpose of the invention is to increase the sensitivity; way.

Example. Determination of quinine hydrochloride in the preparation,

In a separating funnel with a capacity of 25 ml, 1 ml of a 5-10 M solution of sodium salidylate, I ml of a solution of iron nitrate, O, 4 MP 1 nt, 1 ml of 1 hydrochloride are placed and the volume is made up to 5 ml with water. The reaction product is extracted with 5 ml of chloroform for 2 minutes. After complete separation of the liquid after i min. the extractant is separated and photometric. The optical breeze was measured. In a cuvette from a 1 cm layer with a No. 5 light filter. In parallel, a standard quinine hygrochloride solution was analyzed. The comparison solution is chloroform.

The content of quinine hydrochloride in the preparation is determined by the formula 0 00396

Ds.

optical density of the extract in the analysis of the test solution of the drug;

the optical density of the extract when analyzing the standard sample solution 0.00396 quinine hydrochloride content in 1 ml of the standard sample solution. In determining the moisture content of moisture, a preparation of 8–10% „of the preparation was dried at 100–105 ° С in a drying cabinet,

- Table 1 presents the results of the determination of quinine hydrochloride in the preparation at optimal ratios of reagents.

With a confidence factor

0.95, the relative determination error is 0.90.

Example 2. Definition of quinine

hydrochloride in dosage form

composition:

Quinine hydrochloride6.0

1 bilone acid 6.0

Cocoa butter30,0

X where D

 ,

100%

(one)

d ..

0

five

-2

Two balls weighing 1.0 are heated in a water bath with 5 ml of water until the base is melted and filtered into a 100 ml volumetric flask. Extraction with water is carried out a further A times 5 ml each time, the filter is transferred into a volumetric flask and the volume is brought to the mark with distilled water. For the study, take 1.5 ml of the solution and then proceed in the manner described.

The content of quinine hydrochloride in one bead in-grams was determined by the formula (1)

The results of determining the model 5 samples of quinine hydrochloride in the dosage form - balls (-6) are given in table 2.

The results show that the determination error is within the tolerance limits,

PRI and MER 3. Definition of quinine hydrochloride in the product.

In a separatory funnel with a capacity of

 2

25 ml are placed in 1 ml of 510 M sodium salicylate solution, 1 ml of 1–10 W of iron nitrate solution, 0.4 ml of 1 N solution of acetic acid, 1 ml of quinine hydrochloride solution and brought to 5 mp with water. The product is extracted with 5 ml of chloroform for 2 minutes. After complete separation of the liquid, after 1 min, the extract is separated and photometric. Optical density is measured on FEK-56M in a cuvette with a thickness of 1 cm layer with a No. 5 light filter. In parallel, an analysis of a solution of a standard sample of quinine hydrochloride is carried out. The comparison solution is chloroform.

Quinine dihydrochloride was used as a standard sample.

The quinine hydrochloride content in the preparation is determined by the formula.

Table 3 presents the results of quinine determination in the preparation.

The confidence probability of 0.95, the relative determination error is ± 0.80,

The results of determination of quinine dt1-hydrochloride in the dosage form: 50% solution for injection is given in Table A.

Quinine hydrochloride determination results at an optimal minimum of 0.008 M and a maximum of 0.012 M. the concentration of iron nitrate are given in table.5.

Quinine hydrochloride determination results at optimum minimum

five

0

five

SO

55

0.05 M and a maximum 0.1 M concentration of sodium salicylate are given in Table 6.

of the analyzed sample with a color reagent, followed by extraction of the obtained compound with chloroform and photometry of the extract, is only an increase in sensitivity of the method to 0–0.025 ng / mp (in the well-known) 3 µg / ml, used as color reagents

Claims (1)

a mixture of 0.008-0.012 M nitrate solution The formula of the invention for iron and a 0.05-0.1 M solution of sodium salicylate, taken in an equal volume. 10,1482 20,1482 30,1482 Table 1 Table 2 0.80 0.1508 0.76 0.1427 0.77 0.1452 0.1334-1630 TableD 0,4850--0,5150 7,920,120100,007,92100,00 7,920,120100,007,98100,75 7,920,120100,007,92100,00 7,920,121100,867,92100.00 7,920,120100,007,94100,25 7,920,123102,507,92100.00 7,920,122101,668,12102,52 7,920,120100,007,92100,00 Table3 , 90. , 86. TabliDab