SU1168832A1 - Method of quantitatuve determining of spasmolytine - Google Patents

Abstract

THE METHOD OF THE QUALITY DETERMINATION OF SPASMOLITINA by treating an analyte sample with an organic reagent, extracting the obtained compound with chloroform followed by photometric the obtained extract: characterized in that, in order to improve the accuracy and selectivity of the method, 0.5 M mixture of the reagent mixture is used, and 0.5 M mixture of the mixture of the reagent mixture is used. 2 M pyrocatechin solution, taken in a volume ratio of 0.8-1.0: 1.0: 2.2. (L with

Description

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The invention relates to analytical chemistry, in particular to methods for the extraction-photometric determination of derivatives of diphenylacetic acid, in particular, of the spasmolythin-p-diethyl ethyl ethyl ester of 1,1-diphenylacetic acid hydrochloride.

The purpose of the invention is to increase the accuracy and selectivity of the method.

Example 1. Determination of spasmolitin in a preparation of 0.1250 g of the preparation (exact weight) is dissolved in water in a 25 ml volumetric flask. A mixture of 0.5 M solution of sulfate pyrcate catechin, taken in bulk the ratio of 5.8-1.8: 1.0-2.2 and distilled water to 5 ml and then proceed as described in the construction of the calibration curve.

The spasmolitin content is found on the calibration curve.

Construction of the calibration curve.

To the separatory funnel, 0.52, 5 ml of a 1% spasmolitin standard solution is added, a mixture of 0.5 M copper sulfate solution and 2 M pyrocatechin solution, taken in a volume ratio of 0.8-1.8: 1, is added. 0-2.2 and the volume is made up with water to 5 ml and extracted with 5 ml of chloroform. The organic phase is separated, filtered through a folded filter containing 0.5 g of non-aqueous sodium sulfate. The colored extracts are photometrized with FEC-56 M, 0 cm or 2 filter against idling. According to the crawled data, a calibration curve is constructed.

The results of the quantitative determination of spasmolitin in the preparation are presented in Table 1.

Table 1

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Extension table.1

Example 2. Determination of antispasmodic in a one percent solution. Take 1 MP of 1% spasmolitin solution, add a mixture of 0.5 M copper sulfate solution and 2 M pyrocatechol solution, taken in a volume ratio of 0.8-1.8: 1.0-2.2 and water to volume 5 mp and then proceed as described when building the calibration curve.

The content of spasmolitin in the solution is found on the calibration curve ..

The results of the quantitative determination of spasmolitin in a 1% solution are presented in Table 2.

table 2

The results of spasmolitin determination at the maximum and minimum ratios of copper sulfate and pyrocate-chin are presented in Table 3.

Data showing the increase in selectivity and accuracy of the proposed

Table 3

method compared with the known, I presented in table 4 and 5.

Table 4

Table 5

Claims (2)

00 SCHU> METHOD FOR QUANTITATIVE DETERMINATION OF SPASMOLITHIN by treating a sample of an analyte with an organic reagent, extracting the resulting compound with chloroform, followed by photometric analysis of the obtained extract, characterized in that, in order to increase the accuracy and selectivity of the method, a mixture of 0.5 M copper sulfate solution and 2 M solution of pyrocatechol taken in a volume ratio of 0.8-1.0: 1.0: 2.2. □ 5