SK5592000A3 - Determination of cellular growth abnormality - Google Patents
Determination of cellular growth abnormality Download PDFInfo
- Publication number
- SK5592000A3 SK5592000A3 SK559-2000A SK5592000A SK5592000A3 SK 5592000 A3 SK5592000 A3 SK 5592000A3 SK 5592000 A SK5592000 A SK 5592000A SK 5592000 A3 SK5592000 A3 SK 5592000A3
- Authority
- SK
- Slovakia
- Prior art keywords
- cells
- sample
- mcm5
- target polypeptide
- tissue
- Prior art date
Links
- 230000005856 abnormality Effects 0.000 title claims abstract description 28
- 230000010261 cell growth Effects 0.000 title claims abstract description 14
- 210000004027 cell Anatomy 0.000 claims abstract description 193
- 210000001519 tissue Anatomy 0.000 claims abstract description 101
- 101001017545 Homo sapiens DNA replication licensing factor MCM5 Proteins 0.000 claims abstract description 77
- 102100034001 DNA replication licensing factor MCM5 Human genes 0.000 claims abstract description 72
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 71
- 229920001184 polypeptide Polymers 0.000 claims abstract description 66
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 66
- 101001018431 Homo sapiens DNA replication licensing factor MCM7 Proteins 0.000 claims abstract description 54
- 210000003679 cervix uteri Anatomy 0.000 claims abstract description 46
- 102100033711 DNA replication licensing factor MCM7 Human genes 0.000 claims abstract description 41
- 210000000481 breast Anatomy 0.000 claims abstract description 28
- 101000963174 Homo sapiens DNA replication licensing factor MCM3 Proteins 0.000 claims abstract description 24
- 101000583807 Homo sapiens DNA replication licensing factor MCM2 Proteins 0.000 claims abstract description 23
- 102100039606 DNA replication licensing factor MCM3 Human genes 0.000 claims abstract description 22
- 230000004543 DNA replication Effects 0.000 claims abstract description 20
- 101000615280 Homo sapiens DNA replication licensing factor MCM4 Proteins 0.000 claims abstract description 18
- 102100021389 DNA replication licensing factor MCM4 Human genes 0.000 claims abstract description 17
- 101001018484 Homo sapiens DNA replication licensing factor MCM6 Proteins 0.000 claims abstract description 17
- 102100033720 DNA replication licensing factor MCM6 Human genes 0.000 claims abstract description 16
- 210000004369 blood Anatomy 0.000 claims abstract description 15
- 239000008280 blood Substances 0.000 claims abstract description 15
- 239000012530 fluid Substances 0.000 claims abstract description 13
- 210000002700 urine Anatomy 0.000 claims abstract description 12
- 210000001072 colon Anatomy 0.000 claims abstract description 9
- 210000003491 skin Anatomy 0.000 claims abstract description 7
- 210000004072 lung Anatomy 0.000 claims abstract description 6
- 108020004999 messenger RNA Proteins 0.000 claims abstract description 6
- 210000003932 urinary bladder Anatomy 0.000 claims abstract description 6
- 238000000034 method Methods 0.000 claims description 90
- 206010028980 Neoplasm Diseases 0.000 claims description 62
- 238000009739 binding Methods 0.000 claims description 46
- 230000027455 binding Effects 0.000 claims description 43
- 230000002159 abnormal effect Effects 0.000 claims description 38
- 238000012216 screening Methods 0.000 claims description 37
- 230000009870 specific binding Effects 0.000 claims description 35
- 239000012188 paraffin wax Substances 0.000 claims description 27
- 239000000427 antigen Substances 0.000 claims description 21
- 102000036639 antigens Human genes 0.000 claims description 21
- 108091007433 antigens Proteins 0.000 claims description 21
- 230000002062 proliferating effect Effects 0.000 claims description 16
- 238000004458 analytical method Methods 0.000 claims description 12
- 230000000977 initiatory effect Effects 0.000 claims description 12
- -1 Dbf4 Proteins 0.000 claims description 10
- 230000001954 sterilising effect Effects 0.000 claims description 10
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 210000005170 neoplastic cell Anatomy 0.000 claims description 8
- 210000003238 esophagus Anatomy 0.000 claims description 6
- 101000945740 Homo sapiens Cell division cycle 7-related protein kinase Proteins 0.000 claims description 5
- 102000052165 human CDC7 Human genes 0.000 claims description 5
- 210000002784 stomach Anatomy 0.000 claims description 5
- 101100005729 Caenorhabditis elegans cdc-14 gene Proteins 0.000 claims description 4
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 claims description 4
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 claims description 4
- 210000002307 prostate Anatomy 0.000 claims description 4
- 101000583797 Homo sapiens Protein MCM10 homolog Proteins 0.000 claims description 3
- 102100030962 Protein MCM10 homolog Human genes 0.000 claims description 3
- 238000012360 testing method Methods 0.000 abstract description 24
- 210000002966 serum Anatomy 0.000 abstract description 21
- 201000009030 Carcinoma Diseases 0.000 abstract description 20
- 238000001514 detection method Methods 0.000 abstract description 15
- 238000001574 biopsy Methods 0.000 abstract description 11
- 206010039491 Sarcoma Diseases 0.000 abstract description 5
- 210000001165 lymph node Anatomy 0.000 abstract description 5
- 230000001394 metastastic effect Effects 0.000 abstract description 4
- 206010061289 metastatic neoplasm Diseases 0.000 abstract description 4
- 210000001635 urinary tract Anatomy 0.000 abstract description 4
- 210000000867 larynx Anatomy 0.000 abstract description 3
- 206010066476 Haematological malignancy Diseases 0.000 abstract description 2
- 102100027047 Cell division control protein 6 homolog Human genes 0.000 abstract 1
- 101000914465 Homo sapiens Cell division control protein 6 homolog Proteins 0.000 abstract 1
- 210000000621 bronchi Anatomy 0.000 abstract 1
- 238000010186 staining Methods 0.000 description 115
- 239000003656 tris buffered saline Substances 0.000 description 56
- 239000000523 sample Substances 0.000 description 38
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 36
- 238000009595 pap smear Methods 0.000 description 35
- 108090000623 proteins and genes Proteins 0.000 description 33
- 108050006400 Cyclin Proteins 0.000 description 32
- 102000009339 Proliferating Cell Nuclear Antigen Human genes 0.000 description 32
- 102000004169 proteins and genes Human genes 0.000 description 30
- 210000000981 epithelium Anatomy 0.000 description 29
- 108010051862 Methylmalonyl-CoA mutase Proteins 0.000 description 22
- 102100030979 Methylmalonyl-CoA mutase, mitochondrial Human genes 0.000 description 22
- 201000011510 cancer Diseases 0.000 description 19
- 238000012758 nuclear staining Methods 0.000 description 19
- 239000012634 fragment Substances 0.000 description 18
- 239000010410 layer Substances 0.000 description 18
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 17
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 17
- 239000002953 phosphate buffered saline Substances 0.000 description 17
- 108020004414 DNA Proteins 0.000 description 16
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 16
- 229940098773 bovine serum albumin Drugs 0.000 description 16
- 241000283973 Oryctolagus cuniculus Species 0.000 description 15
- 230000036210 malignancy Effects 0.000 description 15
- 230000000284 resting effect Effects 0.000 description 15
- 210000002919 epithelial cell Anatomy 0.000 description 14
- 108010077544 Chromatin Proteins 0.000 description 13
- 210000003483 chromatin Anatomy 0.000 description 13
- 238000010790 dilution Methods 0.000 description 13
- 239000012895 dilution Substances 0.000 description 13
- 241000283707 Capra Species 0.000 description 12
- 102100030960 DNA replication licensing factor MCM2 Human genes 0.000 description 12
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 12
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 12
- 108020004707 nucleic acids Proteins 0.000 description 12
- 102000039446 nucleic acids Human genes 0.000 description 12
- 150000007523 nucleic acids Chemical class 0.000 description 12
- 210000004881 tumor cell Anatomy 0.000 description 12
- 206010008342 Cervix carcinoma Diseases 0.000 description 11
- 101000697544 Homo sapiens SCL-interrupting locus protein Proteins 0.000 description 11
- 102100028029 SCL-interrupting locus protein Human genes 0.000 description 11
- 201000010881 cervical cancer Diseases 0.000 description 11
- 102000013415 peroxidase activity proteins Human genes 0.000 description 11
- 108040007629 peroxidase activity proteins Proteins 0.000 description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 10
- 230000004663 cell proliferation Effects 0.000 description 10
- 230000002380 cytological effect Effects 0.000 description 10
- 238000002474 experimental method Methods 0.000 description 10
- 238000012744 immunostaining Methods 0.000 description 10
- 230000003902 lesion Effects 0.000 description 10
- 208000022361 Human papillomavirus infectious disease Diseases 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 210000004969 inflammatory cell Anatomy 0.000 description 9
- 230000003211 malignant effect Effects 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 210000002536 stromal cell Anatomy 0.000 description 9
- 238000005406 washing Methods 0.000 description 9
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 8
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 8
- 238000003556 assay Methods 0.000 description 8
- 239000003153 chemical reaction reagent Substances 0.000 description 8
- 238000002360 preparation method Methods 0.000 description 8
- 230000035755 proliferation Effects 0.000 description 8
- 239000008096 xylene Substances 0.000 description 8
- 206010006187 Breast cancer Diseases 0.000 description 7
- 206010008263 Cervical dysplasia Diseases 0.000 description 7
- 206010009900 Colitis ulcerative Diseases 0.000 description 7
- 201000006704 Ulcerative Colitis Diseases 0.000 description 7
- SXEHKFHPFVVDIR-UHFFFAOYSA-N [4-(4-hydrazinylphenyl)phenyl]hydrazine Chemical compound C1=CC(NN)=CC=C1C1=CC=C(NN)C=C1 SXEHKFHPFVVDIR-UHFFFAOYSA-N 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 210000002510 keratinocyte Anatomy 0.000 description 7
- 210000004940 nucleus Anatomy 0.000 description 7
- 239000008188 pellet Substances 0.000 description 7
- 238000012546 transfer Methods 0.000 description 7
- 230000001052 transient effect Effects 0.000 description 7
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 208000026310 Breast neoplasm Diseases 0.000 description 6
- 208000011231 Crohn disease Diseases 0.000 description 6
- 206010058314 Dysplasia Diseases 0.000 description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
- 206010025323 Lymphomas Diseases 0.000 description 6
- 230000002238 attenuated effect Effects 0.000 description 6
- 210000003855 cell nucleus Anatomy 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 230000000968 intestinal effect Effects 0.000 description 6
- 238000002372 labelling Methods 0.000 description 6
- 210000004698 lymphocyte Anatomy 0.000 description 6
- 230000010076 replication Effects 0.000 description 6
- 230000035945 sensitivity Effects 0.000 description 6
- 239000008399 tap water Substances 0.000 description 6
- 235000020679 tap water Nutrition 0.000 description 6
- 238000001262 western blot Methods 0.000 description 6
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 5
- 229920004890 Triton X-100 Polymers 0.000 description 5
- 239000011324 bead Substances 0.000 description 5
- 210000001124 body fluid Anatomy 0.000 description 5
- 239000010839 body fluid Substances 0.000 description 5
- 238000012303 cytoplasmic staining Methods 0.000 description 5
- 230000002255 enzymatic effect Effects 0.000 description 5
- 238000011156 evaluation Methods 0.000 description 5
- 102000053540 human MCM5 Human genes 0.000 description 5
- 230000003053 immunization Effects 0.000 description 5
- 238000003018 immunoassay Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 230000004044 response Effects 0.000 description 5
- 239000011534 wash buffer Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical compound CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 description 4
- 208000022559 Inflammatory bowel disease Diseases 0.000 description 4
- 101150053401 ORC2 gene Proteins 0.000 description 4
- 230000018199 S phase Effects 0.000 description 4
- 238000013459 approach Methods 0.000 description 4
- 230000000903 blocking effect Effects 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 230000022131 cell cycle Effects 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 230000004069 differentiation Effects 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 238000001035 drying Methods 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 210000002950 fibroblast Anatomy 0.000 description 4
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 4
- 239000012520 frozen sample Substances 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 230000002757 inflammatory effect Effects 0.000 description 4
- 229910052747 lanthanoid Inorganic materials 0.000 description 4
- 150000002602 lanthanoids Chemical class 0.000 description 4
- 230000005291 magnetic effect Effects 0.000 description 4
- 230000007170 pathology Effects 0.000 description 4
- 238000003752 polymerase chain reaction Methods 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 206010000830 Acute leukaemia Diseases 0.000 description 3
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 3
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 3
- 206010009944 Colon cancer Diseases 0.000 description 3
- 230000006820 DNA synthesis Effects 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 241000283074 Equus asinus Species 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 3
- 201000004681 Psoriasis Diseases 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 229930182558 Sterol Natural products 0.000 description 3
- 108010090804 Streptavidin Proteins 0.000 description 3
- 208000009956 adenocarcinoma Diseases 0.000 description 3
- 125000003275 alpha amino acid group Chemical group 0.000 description 3
- 150000001413 amino acids Chemical class 0.000 description 3
- 238000002306 biochemical method Methods 0.000 description 3
- 230000030944 contact inhibition Effects 0.000 description 3
- 210000000805 cytoplasm Anatomy 0.000 description 3
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 3
- 229960003964 deoxycholic acid Drugs 0.000 description 3
- 238000010494 dissociation reaction Methods 0.000 description 3
- 230000005593 dissociations Effects 0.000 description 3
- 210000004696 endometrium Anatomy 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 210000002615 epidermis Anatomy 0.000 description 3
- 238000000605 extraction Methods 0.000 description 3
- 210000003608 fece Anatomy 0.000 description 3
- 238000000265 homogenisation Methods 0.000 description 3
- 238000002649 immunization Methods 0.000 description 3
- 238000010166 immunofluorescence Methods 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 230000004054 inflammatory process Effects 0.000 description 3
- 210000004962 mammalian cell Anatomy 0.000 description 3
- 210000004877 mucosa Anatomy 0.000 description 3
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000002271 resection Methods 0.000 description 3
- 239000011780 sodium chloride Substances 0.000 description 3
- 206010041823 squamous cell carcinoma Diseases 0.000 description 3
- 210000000130 stem cell Anatomy 0.000 description 3
- 150000003432 sterols Chemical class 0.000 description 3
- 235000003702 sterols Nutrition 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 230000008961 swelling Effects 0.000 description 3
- 210000003437 trachea Anatomy 0.000 description 3
- 230000002485 urinary effect Effects 0.000 description 3
- GEYOCULIXLDCMW-UHFFFAOYSA-N 1,2-phenylenediamine Chemical compound NC1=CC=CC=C1N GEYOCULIXLDCMW-UHFFFAOYSA-N 0.000 description 2
- MPVDXIMFBOLMNW-ISLYRVAYSA-N 7-hydroxy-8-[(E)-phenyldiazenyl]naphthalene-1,3-disulfonic acid Chemical compound OC1=CC=C2C=C(S(O)(=O)=O)C=C(S(O)(=O)=O)C2=C1\N=N\C1=CC=CC=C1 MPVDXIMFBOLMNW-ISLYRVAYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 206010005003 Bladder cancer Diseases 0.000 description 2
- 208000013165 Bowen disease Diseases 0.000 description 2
- 208000019337 Bowen disease of the skin Diseases 0.000 description 2
- 101150061050 CIN1 gene Proteins 0.000 description 2
- 108010047041 Complementarity Determining Regions Proteins 0.000 description 2
- 108091035707 Consensus sequence Proteins 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 229910052693 Europium Inorganic materials 0.000 description 2
- 101001126977 Homo sapiens Methylmalonyl-CoA mutase, mitochondrial Proteins 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 2
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 2
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 2
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 2
- 108010067060 Immunoglobulin Variable Region Proteins 0.000 description 2
- 102000017727 Immunoglobulin Variable Region Human genes 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 208000001126 Keratosis Diseases 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 206010054949 Metaplasia Diseases 0.000 description 2
- 102100034256 Mucin-1 Human genes 0.000 description 2
- 108010008707 Mucin-1 Proteins 0.000 description 2
- 206010033128 Ovarian cancer Diseases 0.000 description 2
- 241001631646 Papillomaviridae Species 0.000 description 2
- 208000009608 Papillomavirus Infections Diseases 0.000 description 2
- 229930040373 Paraformaldehyde Natural products 0.000 description 2
- 102000004142 Trypsin Human genes 0.000 description 2
- 108090000631 Trypsin Proteins 0.000 description 2
- 208000025865 Ulcer Diseases 0.000 description 2
- 208000007097 Urinary Bladder Neoplasms Diseases 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 150000001298 alcohols Chemical class 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 239000002981 blocking agent Substances 0.000 description 2
- 210000001185 bone marrow Anatomy 0.000 description 2
- 208000024207 chronic leukemia Diseases 0.000 description 2
- 239000002299 complementary DNA Substances 0.000 description 2
- 230000009260 cross reactivity Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 239000000539 dimer Substances 0.000 description 2
- 208000035475 disorder Diseases 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- OGPBJKLSAFTDLK-UHFFFAOYSA-N europium atom Chemical compound [Eu] OGPBJKLSAFTDLK-UHFFFAOYSA-N 0.000 description 2
- 239000013604 expression vector Substances 0.000 description 2
- 230000002550 fecal effect Effects 0.000 description 2
- 230000004927 fusion Effects 0.000 description 2
- 230000000762 glandular Effects 0.000 description 2
- 102000052464 human MCM3 Human genes 0.000 description 2
- 102000052599 human MCM7 Human genes 0.000 description 2
- 235000003642 hunger Nutrition 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 230000004807 localization Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000003550 marker Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000015689 metaplastic ossification Effects 0.000 description 2
- 230000003278 mimic effect Effects 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 230000001613 neoplastic effect Effects 0.000 description 2
- 230000037311 normal skin Effects 0.000 description 2
- 101150095319 orc1 gene Proteins 0.000 description 2
- 210000001672 ovary Anatomy 0.000 description 2
- 210000000496 pancreas Anatomy 0.000 description 2
- 229920002866 paraformaldehyde Polymers 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- 230000035935 pregnancy Effects 0.000 description 2
- 230000000750 progressive effect Effects 0.000 description 2
- 201000005825 prostate adenocarcinoma Diseases 0.000 description 2
- 238000003259 recombinant expression Methods 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 210000002163 scaffold cell Anatomy 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 230000037351 starvation Effects 0.000 description 2
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 239000012588 trypsin Substances 0.000 description 2
- 208000022158 tubulovillous adenoma Diseases 0.000 description 2
- 241000701447 unidentified baculovirus Species 0.000 description 2
- 201000005112 urinary bladder cancer Diseases 0.000 description 2
- 239000001993 wax Substances 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
- 108091006112 ATPases Proteins 0.000 description 1
- 208000010507 Adenocarcinoma of Lung Diseases 0.000 description 1
- 208000036832 Adenocarcinoma of ovary Diseases 0.000 description 1
- 102000057290 Adenosine Triphosphatases Human genes 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 208000031295 Animal disease Diseases 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 208000007879 Atypical Squamous Cells of the Cervix Diseases 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 102100026189 Beta-galactosidase Human genes 0.000 description 1
- 206010055113 Breast cancer metastatic Diseases 0.000 description 1
- 101150070189 CIN3 gene Proteins 0.000 description 1
- 208000009458 Carcinoma in Situ Diseases 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 230000033616 DNA repair Effects 0.000 description 1
- 241000283073 Equus caballus Species 0.000 description 1
- 108091060211 Expressed sequence tag Proteins 0.000 description 1
- 241000724791 Filamentous phage Species 0.000 description 1
- 230000010190 G1 phase Effects 0.000 description 1
- 230000004707 G1/S transition Effects 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 208000017604 Hodgkin disease Diseases 0.000 description 1
- 208000021519 Hodgkin lymphoma Diseases 0.000 description 1
- 208000010747 Hodgkins lymphoma Diseases 0.000 description 1
- 241000701806 Human papillomavirus Species 0.000 description 1
- 102000011782 Keratins Human genes 0.000 description 1
- 108010076876 Keratins Proteins 0.000 description 1
- 102000009875 Ki-67 Antigen Human genes 0.000 description 1
- 108010020437 Ki-67 Antigen Proteins 0.000 description 1
- 206010023825 Laryngeal cancer Diseases 0.000 description 1
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 1
- 102000042265 MCM family Human genes 0.000 description 1
- 108091077637 MCM family Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 206010061309 Neoplasm progression Diseases 0.000 description 1
- 208000015914 Non-Hodgkin lymphomas Diseases 0.000 description 1
- 238000000636 Northern blotting Methods 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 102000016304 Origin Recognition Complex Human genes 0.000 description 1
- 108010067244 Origin Recognition Complex Proteins 0.000 description 1
- 206010061328 Ovarian epithelial cancer Diseases 0.000 description 1
- 206010061535 Ovarian neoplasm Diseases 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 108700020962 Peroxidase Proteins 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 108010004729 Phycoerythrin Proteins 0.000 description 1
- 108010039918 Polylysine Proteins 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 208000006994 Precancerous Conditions Diseases 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 206010060862 Prostate cancer Diseases 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 1
- 206010038389 Renal cancer Diseases 0.000 description 1
- 102000006382 Ribonucleases Human genes 0.000 description 1
- 108010083644 Ribonucleases Proteins 0.000 description 1
- 241000277331 Salmonidae Species 0.000 description 1
- 208000000453 Skin Neoplasms Diseases 0.000 description 1
- 208000014604 Specific Language disease Diseases 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- 108091008874 T cell receptors Proteins 0.000 description 1
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 description 1
- 208000024313 Testicular Neoplasms Diseases 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000269370 Xenopus <genus> Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 210000002255 anal canal Anatomy 0.000 description 1
- 230000002494 anti-cea effect Effects 0.000 description 1
- 201000007201 aphasia Diseases 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 210000000270 basal cell Anatomy 0.000 description 1
- 210000002469 basement membrane Anatomy 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 108010005774 beta-Galactosidase Proteins 0.000 description 1
- 210000000013 bile duct Anatomy 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 238000012925 biological evaluation Methods 0.000 description 1
- 210000005068 bladder tissue Anatomy 0.000 description 1
- 238000004159 blood analysis Methods 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 201000008274 breast adenocarcinoma Diseases 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 230000018486 cell cycle phase Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 239000013553 cell monolayer Substances 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 208000016420 cervical intraepithelial neoplasia grade 2/3 Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 150000005829 chemical entities Chemical class 0.000 description 1
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 1
- ZCDOYSPFYFSLEW-UHFFFAOYSA-N chromate(2-) Chemical compound [O-][Cr]([O-])(=O)=O ZCDOYSPFYFSLEW-UHFFFAOYSA-N 0.000 description 1
- 239000003593 chromogenic compound Substances 0.000 description 1
- 101150005988 cin2 gene Proteins 0.000 description 1
- 239000007979 citrate buffer Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 201000010897 colon adenocarcinoma Diseases 0.000 description 1
- 208000029742 colonic neoplasm Diseases 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 238000004624 confocal microscopy Methods 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 210000001608 connective tissue cell Anatomy 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 238000002574 cystoscopy Methods 0.000 description 1
- 230000000120 cytopathologic effect Effects 0.000 description 1
- 101150093523 dbf4 gene Proteins 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000000881 depressing effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- BFMYDTVEBKDAKJ-UHFFFAOYSA-L disodium;(2',7'-dibromo-3',6'-dioxido-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl)mercury;hydrate Chemical compound O.[Na+].[Na+].O1C(=O)C2=CC=CC=C2C21C1=CC(Br)=C([O-])C([Hg])=C1OC1=C2C=C(Br)C([O-])=C1 BFMYDTVEBKDAKJ-UHFFFAOYSA-L 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 230000005274 electronic transitions Effects 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000009841 epithelial lesion Effects 0.000 description 1
- 229940089063 epitol Drugs 0.000 description 1
- 210000005002 female reproductive tract Anatomy 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 210000001280 germinal center Anatomy 0.000 description 1
- 230000001744 histochemical effect Effects 0.000 description 1
- 238000007825 histological assay Methods 0.000 description 1
- 102000048557 human MCM2 Human genes 0.000 description 1
- 102000050409 human MCM4 Human genes 0.000 description 1
- 102000047273 human MCM6 Human genes 0.000 description 1
- 210000005260 human cell Anatomy 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
- 206010020718 hyperplasia Diseases 0.000 description 1
- 230000002390 hyperplastic effect Effects 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000012760 immunocytochemical staining Methods 0.000 description 1
- 238000003125 immunofluorescent labeling Methods 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 230000036046 immunoreaction Effects 0.000 description 1
- 239000003547 immunosorbent Substances 0.000 description 1
- 201000004933 in situ carcinoma Diseases 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 229940055742 indium-111 Drugs 0.000 description 1
- APFVFJFRJDLVQX-AHCXROLUSA-N indium-111 Chemical compound [111In] APFVFJFRJDLVQX-AHCXROLUSA-N 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 208000020082 intraepithelial neoplasia Diseases 0.000 description 1
- 206010073095 invasive ductal breast carcinoma Diseases 0.000 description 1
- 201000010985 invasive ductal carcinoma Diseases 0.000 description 1
- XMBWDFGMSWQBCA-YPZZEJLDSA-N iodane Chemical compound [125IH] XMBWDFGMSWQBCA-YPZZEJLDSA-N 0.000 description 1
- 229940044173 iodine-125 Drugs 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 239000000990 laser dye Substances 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 201000005249 lung adenocarcinoma Diseases 0.000 description 1
- 201000005296 lung carcinoma Diseases 0.000 description 1
- 210000005004 lymphoid follicle Anatomy 0.000 description 1
- 210000005075 mammary gland Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 208000016044 mixed lobular and ductal breast carcinoma Diseases 0.000 description 1
- 238000010369 molecular cloning Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 229940126619 mouse monoclonal antibody Drugs 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 230000017074 necrotic cell death Effects 0.000 description 1
- 230000009826 neoplastic cell growth Effects 0.000 description 1
- 210000000440 neutrophil Anatomy 0.000 description 1
- 210000002445 nipple Anatomy 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 208000013371 ovarian adenocarcinoma Diseases 0.000 description 1
- 201000006588 ovary adenocarcinoma Diseases 0.000 description 1
- 210000000277 pancreatic duct Anatomy 0.000 description 1
- 230000005298 paramagnetic effect Effects 0.000 description 1
- 239000011236 particulate material Substances 0.000 description 1
- 238000003909 pattern recognition Methods 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 229920000656 polylysine Polymers 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012342 propidium iodide staining Methods 0.000 description 1
- 201000001514 prostate carcinoma Diseases 0.000 description 1
- 239000011241 protective layer Substances 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 238000000163 radioactive labelling Methods 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 201000010174 renal carcinoma Diseases 0.000 description 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000012536 storage buffer Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 229960001005 tuberculin Drugs 0.000 description 1
- 230000005751 tumor progression Effects 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 210000000626 ureter Anatomy 0.000 description 1
- 238000002562 urinalysis Methods 0.000 description 1
- 238000005353 urine analysis Methods 0.000 description 1
- 210000004291 uterus Anatomy 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 238000003260 vortexing Methods 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57407—Specifically defined cancers
- G01N33/57411—Specifically defined cancers of cervix
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
- G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
- G01N33/57496—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites involving intracellular compounds
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Pathology (AREA)
- Biotechnology (AREA)
- Oncology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Hospice & Palliative Care (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
- Measuring And Recording Apparatus For Diagnosis (AREA)
- Electrotherapy Devices (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Liquid Crystal Substances (AREA)
- Eye Examination Apparatus (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB9722217.8A GB9722217D0 (en) | 1997-10-21 | 1997-10-21 | Determination of cellular growth abnormality |
| GBGB9724134.3A GB9724134D0 (en) | 1997-11-14 | 1997-11-14 | Determination of cellular growth abnormality |
| GBGB9804156.9A GB9804156D0 (en) | 1998-02-26 | 1998-02-26 | Determination of cellular growth abnormality |
| GBGB9810560.4A GB9810560D0 (en) | 1998-05-15 | 1998-05-15 | Determination of cellular growth abnormality |
| GBGB9817075.6A GB9817075D0 (en) | 1998-08-05 | 1998-08-05 | Determination of cellular growth abnormality |
| PCT/GB1998/003153 WO1999021014A1 (fr) | 1997-10-21 | 1998-10-21 | Determination d'anomalies de la croissance cellulaire |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SK5592000A3 true SK5592000A3 (en) | 2000-10-09 |
Family
ID=27517431
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SK559-2000A SK5592000A3 (en) | 1997-10-21 | 1998-10-21 | Determination of cellular growth abnormality |
Country Status (23)
| Country | Link |
|---|---|
| EP (1) | EP1025444B1 (fr) |
| JP (2) | JP2000511291A (fr) |
| CN (1) | CN1282422A (fr) |
| AT (1) | ATE290213T1 (fr) |
| AU (1) | AU751754B2 (fr) |
| BR (1) | BR9813127A (fr) |
| CA (1) | CA2305872C (fr) |
| CO (1) | CO5080817A1 (fr) |
| DE (1) | DE69829198T2 (fr) |
| DK (1) | DK1025444T3 (fr) |
| ES (1) | ES2239407T3 (fr) |
| GB (1) | GB2332515B (fr) |
| HU (1) | HUP0004134A2 (fr) |
| IL (1) | IL135602A0 (fr) |
| IS (1) | IS5456A (fr) |
| MA (1) | MA26557A1 (fr) |
| NO (1) | NO20002044L (fr) |
| NZ (1) | NZ503996A (fr) |
| PE (1) | PE20000121A1 (fr) |
| PL (1) | PL340057A1 (fr) |
| PT (1) | PT1025444E (fr) |
| SK (1) | SK5592000A3 (fr) |
| WO (1) | WO1999021014A1 (fr) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6361954B1 (en) | 1996-05-02 | 2002-03-26 | Cold Spring Harbor Laboratory | Methods of immunoassay for human CDC6 |
| GB0018140D0 (en) * | 2000-07-24 | 2000-09-13 | Medical Res Council | Screening for abnormalities |
| DE10063179A1 (de) * | 2000-12-18 | 2002-06-20 | Bayer Ag | Verfahren zur spezifischen Detektion von Tumorzellen und ihren Vorstufen in Gebärmutterhalsabstrichen durch simultane Messung von mindestens zwei verschiedenen molekularen Markern |
| DE10063112A1 (de) | 2000-12-18 | 2002-06-20 | Bayer Ag | Verfahren zur Erhöhung der klinischen Spezifität bei der Detektion von Tumoren und ihren Vorstufen durch simultane Messung von mindestens zwei verschiedenen molekularen Markern |
| CN1554025A (zh) | 2001-03-12 | 2004-12-08 | Īŵ���ɷ�����˾ | 患病状态的细胞为基础的检测和鉴别 |
| EP1369694A1 (fr) | 2002-04-09 | 2003-12-10 | MTM Laboratories AG | Procédé de discrimination entre des métaplasies et des lésions néoplastiques ou préneoplastiques |
| US7361511B2 (en) * | 2002-08-20 | 2008-04-22 | Millenium Pharmaceuticals, Inc. | Compositions, kits, and methods for identification, assessment, prevention, and therapy of cervical cancer |
| EP1422526A1 (fr) | 2002-10-28 | 2004-05-26 | MTM Laboratories AG | Procédé ameliorée de diagnostic des dysplasies |
| WO2004074301A2 (fr) * | 2003-02-14 | 2004-09-02 | Smithkline Beecham Corporation | Acides nucleiques a expression differentielle qui sont en correlation avec l'expression ksp |
| US7361460B2 (en) | 2003-04-11 | 2008-04-22 | Digene Corporation | Approach to molecular diagnosis of human papillomavirus-related diseases |
| EP1688503A4 (fr) * | 2003-10-20 | 2007-10-31 | Sysmex Corp | Methode de traitement de cellules |
| KR100882249B1 (ko) * | 2004-03-24 | 2009-02-06 | 트리패스 이미징, 인코포레이티드 | 자궁경부 질환의 검사 방법 및 조성물 |
| US7595380B2 (en) * | 2005-04-27 | 2009-09-29 | Tripath Imaging, Inc. | Monoclonal antibodies and methods for their use in the detection of cervical disease |
| GB0518877D0 (en) * | 2005-09-15 | 2005-10-26 | Medical Res Council | Markers and methods |
| US7632498B2 (en) | 2005-12-19 | 2009-12-15 | Tripath Imaging, Inc. | MCM6 and MCM7 monoclonal antibodies and methods for their use in the detection of cervical disease |
| EP2479285B1 (fr) | 2006-01-05 | 2014-05-14 | The Ohio State University Research Foundation | Procédés à base de micro ARN et compositions pour le diagnostic et le traitement des cancers solides |
| EP1996731A2 (fr) | 2006-03-20 | 2008-12-03 | The Ohio State University Research Foundation | Empreintes digitales micro-arn pendant une mégacaryocytopoïese |
| DE102006020852A1 (de) | 2006-05-04 | 2007-11-15 | Robert Bosch Gmbh | Gasdruckbehälter für gasbetriebene Kraftfahrzeuge |
| EP2653561B1 (fr) | 2007-08-03 | 2016-03-02 | The Ohio State University Research Foundation | Régions ultraconservées codant de l'ARNnm |
| GB0811567D0 (en) | 2008-06-24 | 2008-07-30 | Cytosystems Ltd | Assay |
| CN102803511A (zh) | 2009-11-23 | 2012-11-28 | 俄亥俄州立大学 | 用于影响肿瘤细胞生长、迁移和侵袭的材料和方法 |
| GB0921873D0 (en) * | 2009-12-15 | 2010-01-27 | Cytosystems Ltd | Assay |
| JP5931897B2 (ja) | 2010-11-12 | 2016-06-08 | ジ・オハイオ・ステイト・ユニバーシティ・リサーチ・ファウンデイションＴｈｅ Ｏｈｉｏ Ｓｔａｔｅ Ｕｎｉｖｅｒｓｉｔｙ Ｒｅｓｅａｒｃｈ Ｆｏｕｎｄａｔｉｏｎ | マイクロｒｎａ−２１、ミスマッチ修復および結腸直腸癌に関連する物質および方法 |
| US10758619B2 (en) | 2010-11-15 | 2020-09-01 | The Ohio State University | Controlled release mucoadhesive systems |
| WO2012090479A1 (fr) * | 2010-12-28 | 2012-07-05 | Oncotherapy Science, Inc. | Mcm7 comme gène cible pour la thérapie anticancéreuse et le diagnostic du cancer |
| AU2012323924A1 (en) | 2011-10-14 | 2014-05-29 | The Ohio State University | Methods and materials related to ovarian cancer |
| WO2013090556A1 (fr) | 2011-12-13 | 2013-06-20 | The Ohio State University | Procédés et compositions se rapportant à mir-21 et mir-29a, à l'inhibition d'exosome, et à la métastase cancéreuse |
| CA2860549A1 (fr) * | 2012-01-05 | 2013-07-11 | Christophe Cazaux | Signature pour le diagnostic de l'agressivite et de l'instabilite genetique du cancer du poumon |
| WO2013110053A1 (fr) | 2012-01-20 | 2013-07-25 | The Ohio State University | Signatures de marqueurs biologiques du cancer du sein concernant le pouvoir envahissant et le pronostic |
| US11306364B2 (en) | 2012-02-18 | 2022-04-19 | Duke University | TERT promoter mutations in gliomas and a subset of tumors |
| US10711310B2 (en) * | 2013-02-18 | 2020-07-14 | Duke University | Tert promoter mutations in gliomas and a subset of tumors |
| GB201412731D0 (en) * | 2014-07-17 | 2014-09-03 | Immunocore Ltd & Adaptimmune Ltd | Peptides |
| AU2016261279B2 (en) * | 2015-05-10 | 2020-12-10 | Ventana Medical Systems, Inc. | Compositions and methods for simultaneous inactivation of alkaline phosphatase and peroxidase enzymes during automated multiplex tissue staining assays |
| US11519916B2 (en) | 2015-06-08 | 2022-12-06 | Arquer Diagnostics Limited | Methods for analysing a urine sample |
| GB201509907D0 (en) * | 2015-06-08 | 2015-07-22 | Urosens Ltd | Antibodies |
| ES2911415T3 (es) | 2015-06-08 | 2022-05-19 | Arquer Diagnostics Ltd | Métodos y kits |
| GB201511196D0 (en) | 2015-06-25 | 2015-08-12 | Cytosystems Ltd | Monoclonal antibodies |
| JP6245299B2 (ja) * | 2016-04-27 | 2017-12-13 | バクスアルタ ゲーエムベーハー | 組換え安定細胞クローン、その産生およびその使用 |
| CN106636343B (zh) * | 2016-11-04 | 2021-03-19 | 深圳大学 | 一种检测口腔癌的标志物及其试剂盒 |
| GB201820867D0 (en) * | 2018-12-20 | 2019-02-06 | Arquer Diagnostics Ltd | Detection method |
| CN112946253B (zh) * | 2019-12-11 | 2022-11-08 | 广东菲鹏生物有限公司 | 用于免疫诊断试剂中固相载体的封闭阶段处理剂及应用和产品 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK144485A (da) * | 1984-03-30 | 1985-10-01 | Syntex Inc | Monoklonale antistoffer |
| US5851821A (en) * | 1996-05-02 | 1998-12-22 | Cold Spring Harbor Laboratory | DNA Replication-regulating genes |
| EP0812594A1 (fr) * | 1996-06-13 | 1997-12-17 | BOEHRINGER INGELHEIM INTERNATIONAL GmbH | Composés interférant avec la réplication de l'ADN dans les cellules proliférant rapidement pour l'utilisation dans la thérapie des cancers, et méthodes pour identifier ces composés |
| US5858683A (en) * | 1996-08-30 | 1999-01-12 | Matritech, Inc. | Methods and compositions for the detection of cervical cancer |
-
1998
- 1998-10-21 HU HU0004134A patent/HUP0004134A2/hu unknown
- 1998-10-21 NZ NZ503996A patent/NZ503996A/en not_active IP Right Cessation
- 1998-10-21 ES ES98949123T patent/ES2239407T3/es not_active Expired - Lifetime
- 1998-10-21 PE PE1998000991A patent/PE20000121A1/es not_active Application Discontinuation
- 1998-10-21 PT PT98949123T patent/PT1025444E/pt unknown
- 1998-10-21 SK SK559-2000A patent/SK5592000A3/sk unknown
- 1998-10-21 AT AT98949123T patent/ATE290213T1/de active
- 1998-10-21 GB GB9823069A patent/GB2332515B/en not_active Expired - Lifetime
- 1998-10-21 EP EP98949123A patent/EP1025444B1/fr not_active Expired - Lifetime
- 1998-10-21 PL PL98340057A patent/PL340057A1/xx unknown
- 1998-10-21 MA MA25308A patent/MA26557A1/fr unknown
- 1998-10-21 WO PCT/GB1998/003153 patent/WO1999021014A1/fr active IP Right Grant
- 1998-10-21 IL IL13560298A patent/IL135602A0/xx unknown
- 1998-10-21 AU AU95502/98A patent/AU751754B2/en not_active Expired
- 1998-10-21 CO CO98061237A patent/CO5080817A1/es unknown
- 1998-10-21 CA CA002305872A patent/CA2305872C/fr not_active Expired - Lifetime
- 1998-10-21 BR BR9813127-3A patent/BR9813127A/pt not_active Application Discontinuation
- 1998-10-21 JP JP11523482A patent/JP2000511291A/ja active Pending
- 1998-10-21 CN CN98812478A patent/CN1282422A/zh active Pending
- 1998-10-21 DE DE69829198T patent/DE69829198T2/de not_active Expired - Lifetime
- 1998-10-21 DK DK98949123T patent/DK1025444T3/da active
-
2000
- 2000-04-17 IS IS5456A patent/IS5456A/is unknown
- 2000-04-18 NO NO20002044A patent/NO20002044L/no not_active Application Discontinuation
-
2003
- 2003-01-15 JP JP2003007249A patent/JP3774196B2/ja not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| WO1999021014A1 (fr) | 1999-04-29 |
| DE69829198D1 (de) | 2005-04-07 |
| DK1025444T3 (da) | 2005-06-27 |
| MA26557A1 (fr) | 2004-12-20 |
| IS5456A (is) | 2000-04-17 |
| BR9813127A (pt) | 2000-08-15 |
| DE69829198T2 (de) | 2006-01-12 |
| CN1282422A (zh) | 2001-01-31 |
| HUP0004134A2 (en) | 2001-03-28 |
| PL340057A1 (en) | 2001-01-15 |
| PT1025444E (pt) | 2005-07-29 |
| AU9550298A (en) | 1999-05-10 |
| ES2239407T3 (es) | 2005-09-16 |
| ATE290213T1 (de) | 2005-03-15 |
| IL135602A0 (en) | 2001-05-20 |
| CO5080817A1 (es) | 2001-09-25 |
| AU751754B2 (en) | 2002-08-29 |
| GB2332515A (en) | 1999-06-23 |
| GB2332515B (en) | 1999-12-15 |
| JP2003240786A (ja) | 2003-08-27 |
| GB9823069D0 (en) | 1998-12-16 |
| NZ503996A (en) | 2002-04-26 |
| EP1025444B1 (fr) | 2005-03-02 |
| JP2000511291A (ja) | 2000-08-29 |
| NO20002044L (no) | 2000-06-07 |
| PE20000121A1 (es) | 2000-02-17 |
| EP1025444A1 (fr) | 2000-08-09 |
| JP3774196B2 (ja) | 2006-05-10 |
| CA2305872A1 (fr) | 1999-04-29 |
| CA2305872C (fr) | 2008-02-19 |
| NO20002044D0 (no) | 2000-04-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7056690B2 (en) | Detection of dysplastic or neoplastic cells using anti-MCM2 antibodies | |
| CA2305872C (fr) | Determination d'anomalies de la croissance cellulaire | |
| US11155878B2 (en) | Method for discrimination of metaplasias from neoplastic or preneoplastic lesions | |
| ES2381644T3 (es) | Métodos y composiciones para la detección de enfermedades de cuello uterino | |
| EP1388734B1 (fr) | Méthode de diagnostic en utilisant une solution d'échantillon | |
| JP5711196B2 (ja) | 卵巣癌を判定するためのｈｅ４及び他の生化学マーカーの使用 | |
| US20030219726A1 (en) | Detection of abnormalities leading to cervical malignancy | |
| CA2823460A1 (fr) | Procede diagnostique | |
| JP4190031B2 (ja) | 子宮頸細胞の検定評価 | |
| JP2003526777A (ja) | ＡＧＵＳ・ＰａｐスミアにおけるＭＮ／ＣＡ９タンパク質の発現を用いた診断法 | |
| JP2007502995A (ja) | 卵管特異的糖タンパク質レベルを検出することによる婦人科新生物の診断方法 | |
| CZ20001502A3 (cs) | Způsob stanovení přítomnosti abnormálně proliferujících buněk | |
| MXPA00003892A (en) | Determination of cellular growth abnormality | |
| JP2014534432A (ja) | 乳癌用の予測バイオマーカ | |
| Foster et al. | Identification of Possible Molecular Markers to Predict the Malignant Tendency of the Prostate Intraepithelial Neoplasia (PIN) Lesions |