SG91238A1 - Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme - Google Patents

Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme

Info

Publication number
SG91238A1
SG91238A1 SG9601901A SG1996001901A SG91238A1 SG 91238 A1 SG91238 A1 SG 91238A1 SG 9601901 A SG9601901 A SG 9601901A SG 1996001901 A SG1996001901 A SG 1996001901A SG 91238 A1 SG91238 A1 SG 91238A1
Authority
SG
Singapore
Prior art keywords
nucleic acids
proteolytic enzyme
methods
extracting nucleic
pcr amplification
Prior art date
Application number
SG9601901A
Other languages
English (en)
Inventor
Thomas Joseph Cummins
Tobias Davis Ekeze
Original Assignee
Johnson & Johnson Clin Diag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson & Johnson Clin Diag filed Critical Johnson & Johnson Clin Diag
Publication of SG91238A1 publication Critical patent/SG91238A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/803—Physical recovery methods, e.g. chromatography, grinding
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • AIDS & HIV (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Amplifiers (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
SG9601901A 1989-10-18 1990-10-12 Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme SG91238A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/423,071 US5231015A (en) 1989-10-18 1989-10-18 Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme

Publications (1)

Publication Number Publication Date
SG91238A1 true SG91238A1 (en) 2002-09-17

Family

ID=23677577

Family Applications (1)

Application Number Title Priority Date Filing Date
SG9601901A SG91238A1 (en) 1989-10-18 1990-10-12 Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme

Country Status (11)

Country Link
US (2) US5231015A (ja)
EP (1) EP0428197B1 (ja)
JP (1) JP2533969B2 (ja)
KR (1) KR910008135A (ja)
AT (1) ATE147108T1 (ja)
CA (1) CA2025845A1 (ja)
DE (1) DE69029563T2 (ja)
FI (1) FI905145A0 (ja)
HK (1) HK113997A (ja)
IE (1) IE903743A1 (ja)
SG (1) SG91238A1 (ja)

Families Citing this family (118)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5231015A (en) * 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme
JPH04200400A (ja) * 1990-11-30 1992-07-21 Sanwa Kagaku Kenkyusho Co Ltd ヒト生体由来の試料からのウイルスゲノムの抽出法及び該ウイルスゲノムの検出法
EP0488243A1 (en) * 1990-11-30 1992-06-03 Sanwa Kagaku Kenkyusho Co., Ltd. Method of extracting virus genome from sample derived from living body infected with the virus and detecting the genome
GB9100551D0 (en) * 1991-01-10 1991-02-20 Amersham Int Plc Method and reagent for eliminating analytical interference in enzymatic analysis from substances used for extraction of intracellular metabolites
EP0534640B1 (en) * 1991-09-23 1996-10-02 Pfizer Inc. Process for detecting specific mRNA and DNA in cells
AU664050B2 (en) * 1991-12-18 1995-11-02 Becton Dickinson & Company Process for lysing mycobacteria
DE4212555A1 (de) * 1992-04-15 1993-10-21 Boehringer Mannheim Gmbh Verfahren zum Nachweis von Nukleinsäuren
US5629154A (en) * 1993-11-12 1997-05-13 Geron Corporation Telomerase activity assays
US5837453A (en) * 1992-05-13 1998-11-17 Geron Corporation Telomerase activity assays
EP0698082A4 (en) * 1993-05-06 1997-12-17 Abbott Lab A BUFFER FOR DIRECT LYSIS AND DETECTING HIV-1 PLASMA VIREMIA
CA2121659C (en) * 1993-05-11 2000-11-28 Michael C. Little Sample processing method for mycobacteria
SG63589A1 (en) * 1993-05-14 1999-03-30 Johnson & Johnson Clin Diag Diagnostic compositions elements methods and test kits for amplification and detection of two or more dna's using primers having matched melting temperatures
US5863726A (en) * 1993-11-12 1999-01-26 Geron Corporation Telomerase activity assays
US5804380A (en) * 1993-11-12 1998-09-08 Geron Corporation Telomerase activity assays
KR100230909B1 (ko) * 1993-11-29 1999-12-01 다니엘 엘. 캐시앙 광범위의 유기체로부터 핵산 추출 방법
JP3062250B2 (ja) * 1994-03-10 2000-07-10 ジェン−プローブ・インコーポレイテッド イオン性界面活性剤による、酵素により媒介される反応に対する阻害の抑制方法
US5622822A (en) 1994-09-13 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using polyethyleneimine and an anionic phosphate ester surfactant and amplification of same
US5705366A (en) 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
US5582988A (en) 1994-09-15 1996-12-10 Johnson & Johnson Clinical Diagnostics, Inc. Methods for capture and selective release of nucleic acids using weakly basic polymer and amplification of same
US5538870A (en) * 1994-09-21 1996-07-23 Boehringer Mannheim Corporation Method for preparing nucleic acids for analysis and kits useful therefor
WO1997015687A1 (en) * 1995-06-07 1997-05-01 Geron Corporation Telomerase activity assays
US5989813A (en) * 1995-07-13 1999-11-23 Molecular Innovations, Inc. Detection of amplified nucleic acid sequences using bifunctional haptenization and dyed microparticles
DE19644302A1 (de) * 1995-11-28 1997-06-05 Boehringer Mannheim Gmbh Verfahren zum Nachweis von Telomerase-Aktivität
US5942425A (en) * 1996-03-12 1999-08-24 Walters; Adriann H. Method to access nucleic acids from cells
CA2263492C (en) * 1996-08-23 2006-10-17 Scimed Life Systems, Inc. Stent delivery system having stent securement apparatus
ES2126513B1 (es) * 1997-03-14 1999-12-01 Consejo Superior Investigacion Procedimiento para la deteccion de hongos y bacterias en conductos de aire acondicionado.
US6368871B1 (en) * 1997-08-13 2002-04-09 Cepheid Non-planar microstructures for manipulation of fluid samples
CA2312102C (en) * 1997-12-24 2007-09-04 Cepheid Integrated fluid manipulation cartridge
EP0953635B1 (en) * 1998-04-28 2012-08-29 Ortho-Clinical Diagnostics, Inc. Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
CN1167794C (zh) 1998-09-08 2004-09-22 宝酒造株式会社 Dna的合成方法
US7914994B2 (en) 1998-12-24 2011-03-29 Cepheid Method for separating an analyte from a sample
US6431476B1 (en) 1999-12-21 2002-08-13 Cepheid Apparatus and method for rapid ultrasonic disruption of cells or viruses
AU3377500A (en) * 1999-02-26 2000-09-14 Myriad Genetics, Inc. Method for extracting dna from dried specimens
US6469159B1 (en) 1999-04-26 2002-10-22 Robert T. Belly Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
DE60014399T2 (de) * 1999-05-04 2005-12-08 Ortho-Clinical Diagnostics, Inc. Schnelle und wirksame dns-immobilisierung aus einer probe ohne die verwendung von zelllysereagenzien
US9073053B2 (en) 1999-05-28 2015-07-07 Cepheid Apparatus and method for cell disruption
US20040200909A1 (en) * 1999-05-28 2004-10-14 Cepheid Apparatus and method for cell disruption
US8815521B2 (en) 2000-05-30 2014-08-26 Cepheid Apparatus and method for cell disruption
EP1208189B1 (en) 1999-05-28 2004-10-06 Cepheid Device and method for analysing liquid samples
US6664104B2 (en) 1999-06-25 2003-12-16 Cepheid Device incorporating a microfluidic chip for separating analyte from a sample
US6383783B1 (en) 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
DE19954181A1 (de) * 1999-11-10 2001-05-31 Eppendorf Geraetebau Netheler Verwendung nichtionogener Tenside zur Durchführung enzymatischer Reaktionen
AU4312201A (en) * 1999-12-10 2001-06-18 Genespan Corporation Isolation and purification of nucleic acids
US7262006B1 (en) 2000-05-01 2007-08-28 Ortho-Clinical Diagnostics, Inc. Rapid and efficient capture of DNA from sample without using cell lysing reagent
KR20020029477A (ko) * 2000-10-13 2002-04-19 박제철 한 단계에 의한 유전자 추출방법
KR20020029476A (ko) * 2000-10-13 2002-04-19 박제철 한 단계에 의해 유전자를 추출하기 위한 라이시스버퍼시약
KR100426544B1 (ko) * 2000-12-08 2004-04-13 바이오코아 주식회사 혈청 또는 혈장으로부터 바이러스의 핵산을 추출하는 방법및 그 방법에 사용되는 핵산 추출용 조성물
US7074333B2 (en) 2001-01-19 2006-07-11 Millipore Corporation Recovery of linear nucleic acids by salt dilution and/or reduced pressure prior to continuous pressure differential ultrafiltration
FR2822477B1 (fr) * 2001-03-23 2003-08-08 Inst Nat Sante Rech Med Methode de genotypage sans extraction de l'adn
US20040106109A1 (en) * 2001-10-02 2004-06-03 Belly Robert T Detection of ras mutations
US20040038213A1 (en) * 2002-08-06 2004-02-26 Kwon Jai W. Genotyping by in situ PCR amplification of a polynucleotide in a tissue biopsy
KR100481357B1 (ko) * 2002-10-28 2005-04-07 주식회사 바이오세움 바이러스 핵산 추출용 조성물
WO2005011867A2 (en) 2003-07-31 2005-02-10 Handylab, Inc. Processing particle-containing samples
US20050042660A1 (en) * 2003-07-31 2005-02-24 Hall Gerald Edward Devices and methods for isolating RNA
JP2007503958A (ja) 2003-09-03 2007-03-01 ライフパッチ インターナショナル，インコーポレイテッド 個人診断装置と関連手法
US20050130177A1 (en) 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
US7727710B2 (en) 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface
US7939249B2 (en) 2003-12-24 2011-05-10 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
US8852862B2 (en) 2004-05-03 2014-10-07 Handylab, Inc. Method for processing polynucleotide-containing samples
WO2006004611A2 (en) * 2004-06-25 2006-01-12 Invitrogen Corporation Separation of nucleic acid
US20060099605A1 (en) * 2004-11-11 2006-05-11 Hall Gerald E Jr Devices and methods for isolating RNA
EP2333561A3 (en) 2005-03-10 2014-06-11 Gen-Probe Incorporated System for performing multi-formatted assays
EP2348320B1 (en) 2005-03-10 2024-05-01 Gen-Probe Incorporated Methods and systems for detecting multiple fluorescent emission signals
US8628918B2 (en) * 2005-05-09 2014-01-14 Affymetrix, Inc. Multiplex capture of nucleic acids
US8632970B2 (en) 2005-05-09 2014-01-21 Affymetrix, Inc. Multiplex capture of nucleic acids
EP1880025B1 (en) 2005-05-12 2011-03-16 Affymetrix, Inc. Multiplex branched-chain dna assays
US20060269929A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods and kits for DNA purification on polymeric membranes at low ionic strength
US20060270843A1 (en) * 2005-05-26 2006-11-30 Hall Gerald E Jr Methods for isolation of nucleic acids
CN104673905B (zh) * 2005-06-20 2016-09-07 领先细胞医疗诊断有限公司 检测单个细胞中的核酸和鉴定异质大细胞群中罕见细胞的方法
US7927798B2 (en) * 2005-10-05 2011-04-19 Panomics, Inc. Detection of nucleic acids from whole blood
US7998708B2 (en) 2006-03-24 2011-08-16 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US10900066B2 (en) 2006-03-24 2021-01-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
EP2001990B1 (en) 2006-03-24 2016-06-29 Handylab, Inc. Integrated system for processing microfluidic samples, and method of using same
US11806718B2 (en) 2006-03-24 2023-11-07 Handylab, Inc. Fluorescence detector for microfluidic diagnostic system
EP2019687B1 (en) 2006-03-29 2014-03-19 Merial Limited Vaccine against streptococci
EP2091647A2 (en) 2006-11-14 2009-08-26 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
WO2008060604A2 (en) 2006-11-14 2008-05-22 Handylab, Inc. Microfluidic system for amplifying and detecting polynucleotides in parallel
US9186677B2 (en) 2007-07-13 2015-11-17 Handylab, Inc. Integrated apparatus for performing nucleic acid extraction and diagnostic testing on multiple biological samples
US8287820B2 (en) 2007-07-13 2012-10-16 Handylab, Inc. Automated pipetting apparatus having a combined liquid pump and pipette head system
US8182763B2 (en) 2007-07-13 2012-05-22 Handylab, Inc. Rack for sample tubes and reagent holders
US8105783B2 (en) 2007-07-13 2012-01-31 Handylab, Inc. Microfluidic cartridge
US8324372B2 (en) 2007-07-13 2012-12-04 Handylab, Inc. Polynucleotide capture materials, and methods of using same
US8545857B2 (en) * 2008-10-31 2013-10-01 The Invention Science Fund I, Llc Compositions and methods for administering compartmentalized frozen particles
US9060931B2 (en) 2008-10-31 2015-06-23 The Invention Science Fund I, Llc Compositions and methods for delivery of frozen particle adhesives
US8788211B2 (en) 2008-10-31 2014-07-22 The Invention Science Fund I, Llc Method and system for comparing tissue ablation or abrasion data to data related to administration of a frozen particle composition
US9050251B2 (en) 2008-10-31 2015-06-09 The Invention Science Fund I, Llc Compositions and methods for delivery of frozen particle adhesives
US8551506B2 (en) * 2008-10-31 2013-10-08 The Invention Science Fund I, Llc Compositions and methods for administering compartmentalized frozen particles
US8568363B2 (en) 2008-10-31 2013-10-29 The Invention Science Fund I, Llc Frozen compositions and methods for piercing a substrate
US8725420B2 (en) 2008-10-31 2014-05-13 The Invention Science Fund I, Llc Compositions and methods for surface abrasion with frozen particles
US9072799B2 (en) 2008-10-31 2015-07-07 The Invention Science Fund I, Llc Compositions and methods for surface abrasion with frozen particles
US8721583B2 (en) 2008-10-31 2014-05-13 The Invention Science Fund I, Llc Compositions and methods for surface abrasion with frozen particles
US9072688B2 (en) * 2008-10-31 2015-07-07 The Invention Science Fund I, Llc Compositions and methods for therapeutic delivery with frozen particles
US8731840B2 (en) * 2008-10-31 2014-05-20 The Invention Science Fund I, Llc Compositions and methods for therapeutic delivery with frozen particles
US8603495B2 (en) 2008-10-31 2013-12-10 The Invention Science Fund I, Llc Compositions and methods for biological remodeling with frozen particle compositions
US8762067B2 (en) 2008-10-31 2014-06-24 The Invention Science Fund I, Llc Methods and systems for ablation or abrasion with frozen particles and comparing tissue surface ablation or abrasion data to clinical outcome data
US9060926B2 (en) 2008-10-31 2015-06-23 The Invention Science Fund I, Llc Compositions and methods for therapeutic delivery with frozen particles
US9060934B2 (en) 2008-10-31 2015-06-23 The Invention Science Fund I, Llc Compositions and methods for surface abrasion with frozen particles
US8545855B2 (en) 2008-10-31 2013-10-01 The Invention Science Fund I, Llc Compositions and methods for surface abrasion with frozen particles
US8731842B2 (en) 2008-10-31 2014-05-20 The Invention Science Fund I, Llc Compositions and methods for biological remodeling with frozen particle compositions
US8731841B2 (en) 2008-10-31 2014-05-20 The Invention Science Fund I, Llc Compositions and methods for therapeutic delivery with frozen particles
US9050070B2 (en) 2008-10-31 2015-06-09 The Invention Science Fund I, Llc Compositions and methods for surface abrasion with frozen particles
US20100111857A1 (en) 2008-10-31 2010-05-06 Boyden Edward S Compositions and methods for surface abrasion with frozen particles
US9050317B2 (en) 2008-10-31 2015-06-09 The Invention Science Fund I, Llc Compositions and methods for therapeutic delivery with frozen particles
US8409376B2 (en) 2008-10-31 2013-04-02 The Invention Science Fund I, Llc Compositions and methods for surface abrasion with frozen particles
US8793075B2 (en) 2008-10-31 2014-07-29 The Invention Science Fund I, Llc Compositions and methods for therapeutic delivery with frozen particles
US8518031B2 (en) 2008-10-31 2013-08-27 The Invention Science Fund I, Llc Systems, devices and methods for making or administering frozen particles
US8551505B2 (en) 2008-10-31 2013-10-08 The Invention Science Fund I, Llc Compositions and methods for therapeutic delivery with frozen particles
WO2011028887A2 (en) * 2009-09-03 2011-03-10 Becton, Dickinson And Company Methods and compositions for direct chemical lysis
CN107365847A (zh) 2010-10-21 2017-11-21 领先细胞医疗诊断有限公司 用于原位检测核酸的超灵敏方法
US8563298B2 (en) 2010-10-22 2013-10-22 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
US8409807B2 (en) 2010-10-22 2013-04-02 T2 Biosystems, Inc. NMR systems and methods for the rapid detection of analytes
CA3155334A1 (en) 2010-10-22 2012-04-26 T2 Biosystems, Inc. NMR SYSTEMS AND METHODS FOR RAPID ANALYTE DETECTION
EP2652147B1 (en) * 2010-12-17 2017-01-25 Wisconsin Alumni Research Foundation One-step method of elution of dna from blood samples
ES2617599T3 (es) 2011-04-15 2017-06-19 Becton, Dickinson And Company Termociclador microfluídico de exploración en tiempo real y métodos para termociclado sincronizado y detección óptica de exploración
EP2773892B1 (en) 2011-11-04 2020-10-07 Handylab, Inc. Polynucleotide sample preparation device
US9562271B2 (en) 2012-04-20 2017-02-07 T2 Biosystems, Inc. Compositions and methods for detection of Candida species
EP3913068B1 (en) 2015-10-12 2023-08-02 Advanced Cell Diagnostics, Inc. In situ detection of nucleotide variants in high noise samples, and compositions and methods related thereto
JP7523776B2 (ja) 2016-01-21 2024-07-29 ティー２ バイオシステムズ，インコーポレーテッド 細菌を迅速に検出するｎｍｒ法及びシステム

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989000577A1 (en) * 1987-07-22 1989-01-26 Imperial Cancer Research Technology Ltd. Digestion method

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4677054A (en) * 1983-08-08 1987-06-30 Sloan-Kettering Institute For Cancer Research Method for simple analysis of relative nucleic acid levels in multiple small samples by cytoplasmic dot hybridization
US4750982A (en) * 1985-03-21 1988-06-14 Lifecodes Corp. Process and apparatus for purifying and concentrating DNA from crude mixtures containing DNA
CA1284931C (en) * 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
US4900677A (en) * 1986-09-26 1990-02-13 E. I. Du Pont De Nemours And Company Process for rapid isolation of high molecular weight DNA
DE68913658T3 (de) * 1988-11-11 2005-07-21 Stratagene, La Jolla Klonierung von Immunglobulin Sequenzen aus den variablen Domänen
US5334499A (en) * 1989-04-17 1994-08-02 Eastman Kodak Company Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction
US5231015A (en) * 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1989000577A1 (en) * 1987-07-22 1989-01-26 Imperial Cancer Research Technology Ltd. Digestion method

Also Published As

Publication number Publication date
EP0428197A2 (en) 1991-05-22
JP2533969B2 (ja) 1996-09-11
FI905145A0 (fi) 1990-10-18
DE69029563D1 (de) 1997-02-13
EP0428197A3 (en) 1991-05-29
JPH03133379A (ja) 1991-06-06
EP0428197B1 (en) 1997-01-02
CA2025845A1 (en) 1991-04-19
US5231015A (en) 1993-07-27
ATE147108T1 (de) 1997-01-15
IE903743A1 (en) 1991-04-24
US5543305A (en) 1996-08-06
DE69029563T2 (de) 1997-05-22
HK113997A (en) 1997-08-29
KR910008135A (ko) 1991-05-30

Similar Documents

Publication Publication Date Title
SG91238A1 (en) Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme
RU97113529A (ru) Модифицированная термостабильная днк-полимераза
US9481872B2 (en) Nucleic acid-free thermostable enzymes and methods of production thereof
GR3023862T3 (en) High temperature reverse transcriptases
SE8801070D0 (sv) Method for immobilizing a dna sequence on a solid support
DK0620282T3 (da) Fremgangsmåde til ekstraktion, amplificering og påvisning af en nukleinsyre fra helblod
ATE154072T1 (de) Gereinigtes thermostabiles enzym und verfahren zur amplifikation, zum nachweis und/oder zur clonierung von nukleinsäuresequenzen unter verwendung dieses enzyms
EP1350841A3 (en) Thermostable or thermoactive DNA polymerase with attenuated 3'-5' exonuclease activity
EP1396540A3 (en) Direct cloning of PCR amplified nucleic acids
ES2136730T3 (es) Adn polimerasas que presentan una extension de cebado de termoestabilidad, de longitud y de eficacia mejoradas.
GR3035477T3 (en) Thermostable nucleic acid polymerase.
CA2127188A1 (en) Reagents and Methods for Coupled High Temperature Reverse Transcription and Polymerase Chain Reactions
CA2270023A1 (en) Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
CA2121659A1 (en) Sample Processing Method for Mycobacteria
WO2002088387A3 (en) Amplification process
Sakano et al. Sequential processing of precursor tRNA molecules in Escherichia coli.
DE69737024D1 (en) Thermostabiles nukleinsäure-polymerase von thermococcus gorgonarius
DE69222897D1 (de) Verfahren zur Beherrschung der Verunreinigung von Nukleinsäure-Amplifizierungsreaktionen
WO1990009457A3 (en) Oligonucleotide primer pairs for sequence independent gene amplification and methods which employ them
Siedlecki et al. Evidence implying DNA polymerase β function in excision repair
Cummins et al. Methods of extracting deoxyribonucleic acids without using a proteolytic enzyme
EP1004676A4 (en) METHODS FOR DNA REPRODUCTION AND MATERIAL FOR THIS
MX9707885A (es) Metodo para amplificar secuencias especificas de acido nucleico.