SG179157A1 - A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp. - Google Patents
A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp.Info
- Publication number
- SG179157A1 SG179157A1 SG2012018370A SG2012018370A SG179157A1 SG 179157 A1 SG179157 A1 SG 179157A1 SG 2012018370 A SG2012018370 A SG 2012018370A SG 2012018370 A SG2012018370 A SG 2012018370A SG 179157 A1 SG179157 A1 SG 179157A1
- Authority
- SG
- Singapore
- Prior art keywords
- scenedesmus
- dunaliella
- transformation
- algae
- chloroplast genome
- Prior art date
Links
- 241000195663 Scenedesmus Species 0.000 title abstract 3
- 230000009466 transformation Effects 0.000 title abstract 2
- 241001560459 Dunaliella sp. Species 0.000 title 1
- 210000003763 chloroplast Anatomy 0.000 title 1
- 241000195493 Cryptophyta Species 0.000 abstract 3
- 241000195634 Dunaliella Species 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 239000013598 vector Substances 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 241000894007 species Species 0.000 abstract 1
- 238000000844 transformation Methods 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
- C12N15/8207—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated by mechanical means, e.g. microinjection, particle bombardment, silicon whiskers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8214—Plastid transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
- C12N9/242—Fungal source
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US24273509P | 2009-09-15 | 2009-09-15 | |
| PCT/US2010/048828 WO2011034863A1 (en) | 2009-09-15 | 2010-09-14 | A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG179157A1 true SG179157A1 (en) | 2012-05-30 |
Family
ID=43758979
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG2012018370A SG179157A1 (en) | 2009-09-15 | 2010-09-14 | A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp. |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20120252054A1 (https=) |
| EP (1) | EP2477478B1 (https=) |
| CN (1) | CN102630140A (https=) |
| AU (1) | AU2010295739B2 (https=) |
| CA (1) | CA2773675A1 (https=) |
| IN (1) | IN2012DN01694A (https=) |
| SG (1) | SG179157A1 (https=) |
| WO (1) | WO2011034863A1 (https=) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TW201412988A (zh) | 2009-06-17 | 2014-04-01 | Danisco Us Inc | 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造 |
| CA2816306A1 (en) | 2010-10-27 | 2012-05-03 | Danisco Us Inc. | Isoprene synthase variants for improved production of isoprene |
| BR112013018316A2 (pt) | 2010-12-22 | 2018-09-11 | Danisco Us Inc | composições e métodos para produção aprimorada de isopreno usando dois tipos de enzimas ispg |
| JP2014502844A (ja) | 2010-12-22 | 2014-02-06 | ダニスコ・ユーエス・インク | 組み換え細胞による五炭糖の生物学的生産 |
| EP2655625B1 (en) | 2010-12-23 | 2017-01-18 | ExxonMobil Research and Engineering Company | Polypeptides having lipolytic activity, recombinant microorganisms containing exogeneous polypeptides having lipolytic activity and production of fatty acids and fatty acid derivatives thereby |
| SG194691A1 (en) | 2011-04-29 | 2013-12-30 | Danisco Us Inc | Production of mevalonate, isoprene, and isoprenoids using genes encodingpolypeptides having thiolase, hmg-coa synthase and hmg-coa reductase enzymatic activities |
| BR112013027555A2 (pt) | 2011-04-29 | 2021-08-10 | Danisco Us Inc. | células recombinantes capazes de maior produção de isopreno, mevalonato e isoprenoides e método para produzir isopreno, mevalonato e isoprenoides |
| BR112014002661A2 (pt) | 2011-08-04 | 2019-09-24 | Danisco Us Inc | produção de isopreno, precursores de isoprenoide, e isoprenoides com o uso de acetoacetil-coa sintase |
| BR112014008278A2 (pt) | 2011-10-07 | 2017-04-18 | Danisco Us Inc | utilização de fosfocetolase na produção de mevalonato, precursores isoprenoides e isopreno |
| JP2015510392A (ja) | 2011-12-23 | 2015-04-09 | ダニスコ・ユーエス・インク | 低減したｉｓｐａ活性を有する宿主細胞を用いるイソプレン産生増強法 |
| US8865442B2 (en) | 2011-12-23 | 2014-10-21 | Danisco Us Inc. | Production of isoprene under reduced oxygen inlet levels |
| US9315831B2 (en) | 2012-03-30 | 2016-04-19 | Danisco Us Inc. | Direct starch to fermentable sugar as feedstock for the production of isoprene, isoprenoid precursor molecules, and/or isoprenoids |
| US8895277B2 (en) | 2012-05-02 | 2014-11-25 | Danisco Us Inc. | Legume isoprene synthase for production of isoprene |
| US9163263B2 (en) | 2012-05-02 | 2015-10-20 | The Goodyear Tire & Rubber Company | Identification of isoprene synthase variants with improved properties for the production of isoprene |
| EP2909304A4 (en) | 2012-10-16 | 2016-09-21 | Exxonmobil Res & Eng Co | DGAT GENE AND METHOD FOR USE IN TRIGLYCERIDE PRODUCTION IN RECOMBINANT MICROORGANISMS |
| EP2925870A4 (en) * | 2012-12-03 | 2016-07-13 | Adi Zaltsman | PLANT WITH NITROGEN STAINING BY IMPROVING PROKARYOTIC SIGNAL PATHS |
| EP2929030B1 (en) | 2012-12-04 | 2018-10-31 | ExxonMobil Research and Engineering Company | Tetraselmis promoters and terminators for use in eukaryotic cells |
| WO2014088563A1 (en) | 2012-12-05 | 2014-06-12 | Exxonmobil Research And Engineering Company | Nannochloropsis kozak consensus sequence |
| US8835149B2 (en) | 2012-12-06 | 2014-09-16 | Exxonmobil Research And Engineering Company | DGAT genes comprising pleckstrin homology domains and methods of use for triglyceride production in recombinant microorganisms |
| US20160002672A1 (en) | 2012-12-21 | 2016-01-07 | Danisco Us Inc. | Production of isoprene, isoprenoid, and isoprenoid precursors using an alternative lower mevalonate pathway |
| SG11201508186XA (en) | 2013-04-10 | 2015-11-27 | Danisco Us Inc | Phosphoketolases for improved production of acetyl coenzyme a-derived metabolites, isoprene, isoprenoid precursors, and isoprenoids |
| US10648004B2 (en) | 2014-02-20 | 2020-05-12 | Danisco Us Inc. | Recombinant microorganisms for the enhanced production of mevalonate, isoprene, isoprenoid precursors, isoprenoids, and acetyl-CoA-derived products |
| FR3041351B1 (fr) * | 2015-09-17 | 2020-01-24 | Eranova | Procede de preparation d'une poudre d'algues a teneur reduite en proteines et composition bioplastique formulee a partir d'une telle poudre |
| WO2018098001A1 (en) | 2016-11-27 | 2018-05-31 | Triton Algae Innovations, Inc. | Method of purification of recombinant osteopontin from microalgae |
| WO2018203947A2 (en) | 2017-02-06 | 2018-11-08 | Zymergen Inc. | Engineered biosynthetic pathways for production of tyramine by fermentation |
| CN106755537B (zh) * | 2017-03-02 | 2021-04-02 | 云南纳博生物科技有限公司 | 一种准确检测叶绿体转化同质化程度的方法 |
| US11452967B2 (en) | 2017-07-17 | 2022-09-27 | Zymergen Inc. | Metal-organic framework materials |
| JP2020536993A (ja) | 2017-10-05 | 2020-12-17 | ザイマージェン インコーポレイテッド | 光学的に透明なポリイミド |
| CN107988129B (zh) * | 2017-11-27 | 2020-07-14 | 浙江大学 | 改良螺旋藻关键酶基因提高生长固碳速率的方法 |
| WO2019113387A1 (en) | 2017-12-07 | 2019-06-13 | Zymergen Inc. | Engineered biosynthetic pathways for production of (6e)-8-hydroxygeraniol by fermentation |
| JP2021506316A (ja) | 2017-12-21 | 2021-02-22 | ザイマージェン インコーポレイテッド | ネペタラクトールオキシドレダクターゼ、ネペタラクトールシンターゼ、及びネペタラクトンを産生する能力がある微生物 |
| US11718857B2 (en) * | 2019-08-09 | 2023-08-08 | Alliance For Sustainable Energy, Llc | Broad host range genetic tools for engineering microalgae |
| EP4061936A1 (en) | 2019-11-20 | 2022-09-28 | Corbion Biotech, Inc. | Sucrose invertase variants |
| CN110747225B (zh) * | 2019-12-24 | 2020-04-28 | 中国科学院烟台海岸带研究所 | 一种斜生栅藻叶绿体同源重组空载体及其应用 |
| CN115807021A (zh) * | 2022-08-01 | 2023-03-17 | 深圳大学 | 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法 |
| CN115820780A (zh) * | 2022-11-24 | 2023-03-21 | 云南爱尔发生物技术股份有限公司 | 一种利用褪黑素联合乙烯利促进栅藻积累叶黄素的方法 |
| CN118475621A (zh) | 2023-06-19 | 2024-08-09 | 杭州恩和生物科技有限公司 | 生产7-脱氢胆固醇的融合多肽及其使用方法 |
| CN118691958B (zh) * | 2024-06-11 | 2025-01-10 | 广东锐创生态科技有限公司 | 一种基于机器视觉的水产区域监控分析方法及相关装置 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070065900A1 (en) * | 2005-09-19 | 2007-03-22 | Dicosimo Deana J | Process for chromosomal expression of foreign genes in the cytochrome-C peroxidase (cytCP) region of a methylotrophic microbial host cell |
| WO2007133558A2 (en) * | 2006-05-09 | 2007-11-22 | The Scripps Research Institute | Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast |
| US7947478B2 (en) * | 2006-06-29 | 2011-05-24 | The Regents Of The University Of California | Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria |
| CN101479378A (zh) * | 2006-06-29 | 2009-07-08 | 加利福尼亚大学董事会 | 使用经遗传工程改造的微观藻类、蓝细菌或细菌生产短链挥发烃类 |
| CN103298942A (zh) * | 2007-04-03 | 2013-09-11 | 桌面能源公司 | 用于转化真核藻类的方法 |
| AU2008299020A1 (en) * | 2007-09-11 | 2009-03-19 | Sapphire Energy, Inc. | Molecule production by photosynthetic organisms |
| US20090176272A1 (en) * | 2007-09-12 | 2009-07-09 | Kuehnle Agrosystems, Inc. | Expression of nucleic acid sequences for production of biofuels and other products in algae and cyanobacteria |
| CA2698627A1 (en) * | 2007-10-05 | 2009-04-09 | Sapphire Energy, Inc. | System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts |
| WO2010051489A1 (en) * | 2008-10-31 | 2010-05-06 | Sapphire Energy, Inc. | Animal feedstock comprising genetically modified algae |
| EP2381761A4 (en) * | 2008-12-31 | 2012-06-20 | Sapphire Energy Inc | GENETICALLY MODIFIED ALGAE RESISTANT TO HERBICIDES |
| BRPI1008958A2 (pt) * | 2009-03-11 | 2015-09-01 | Sapphire Energy Inc | Produção de biocombustível em procariotas e eucariotas |
| WO2010111707A1 (en) * | 2009-03-27 | 2010-09-30 | Sapphire Energy, Inc. | Variant isoprenoid producing enzymes and uses thereof |
-
2010
- 2010-09-14 US US13/496,149 patent/US20120252054A1/en not_active Abandoned
- 2010-09-14 CA CA2773675A patent/CA2773675A1/en not_active Abandoned
- 2010-09-14 AU AU2010295739A patent/AU2010295739B2/en not_active Ceased
- 2010-09-14 IN IN1694DEN2012 patent/IN2012DN01694A/en unknown
- 2010-09-14 CN CN2010800525438A patent/CN102630140A/zh active Pending
- 2010-09-14 WO PCT/US2010/048828 patent/WO2011034863A1/en not_active Ceased
- 2010-09-14 EP EP10817726.2A patent/EP2477478B1/en not_active Not-in-force
- 2010-09-14 SG SG2012018370A patent/SG179157A1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP2477478B1 (en) | 2016-11-30 |
| CA2773675A1 (en) | 2011-03-24 |
| US20120252054A1 (en) | 2012-10-04 |
| EP2477478A4 (en) | 2013-02-13 |
| EP2477478A1 (en) | 2012-07-25 |
| WO2011034863A1 (en) | 2011-03-24 |
| IN2012DN01694A (https=) | 2015-06-05 |
| AU2010295739A1 (en) | 2012-03-08 |
| AU2010295739B2 (en) | 2016-02-25 |
| CN102630140A (zh) | 2012-08-08 |
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