BR112015012811A2 - genes dgat compreendendo domínios de homologia de plecstrina e uso em micro-organismos recombinantes - Google Patents

genes dgat compreendendo domínios de homologia de plecstrina e uso em micro-organismos recombinantes

Info

Publication number
BR112015012811A2
BR112015012811A2 BR112015012811A BR112015012811A BR112015012811A2 BR 112015012811 A2 BR112015012811 A2 BR 112015012811A2 BR 112015012811 A BR112015012811 A BR 112015012811A BR 112015012811 A BR112015012811 A BR 112015012811A BR 112015012811 A2 BR112015012811 A2 BR 112015012811A2
Authority
BR
Brazil
Prior art keywords
dgat
genes
domains
plecstrin
present
Prior art date
Application number
BR112015012811A
Other languages
English (en)
Inventor
Coppersmith Jennifer
Seshadri Rekha
Howard Richardson Toby
Original Assignee
Exxonmobil Res & Eng Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exxonmobil Res & Eng Co filed Critical Exxonmobil Res & Eng Co
Publication of BR112015012811A2 publication Critical patent/BR112015012811A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6463—Glycerides obtained from glyceride producing microorganisms, e.g. single cell oil
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/0102—Diacylglycerol O-acyltransferase (2.3.1.20)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

abstract the present invention provides novel diacylglycerol acyltransferase (dgat) genes comprising pleckstrin homology (ph) domains. the present invention also provides for recombinant cells, such as algae, transformed with acyltransferase genes, such as dgat, comprising ph domains, and methods of using such recombinant cells to produce increased triglyceride levels. _______________ tradução do resumo resumo patente de invenção: "genes dgat compreendendo domí-nios de homologia de plecstrina e uso em micro-organismos recombinantes". a presente invenção proporciona novos genes de diacilgli-cerol aciltransferase (dgat) que compreendem domínios de homolo-gia de plecstrina (ph). a presente invenção também proporciona célu-las recombinantes, tais como algas, transformadas com genes de acil-transferase, tais como dgat, compreendendo domínios ph, e méto-dos de uso de tais células recombinantes para produzir o aumento dos níveis de triglicerídeos.
BR112015012811A 2012-12-06 2012-12-06 genes dgat compreendendo domínios de homologia de plecstrina e uso em micro-organismos recombinantes BR112015012811A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US13/707,287 US8835149B2 (en) 2012-12-06 2012-12-06 DGAT genes comprising pleckstrin homology domains and methods of use for triglyceride production in recombinant microorganisms
PCT/US2012/068272 WO2014088583A1 (en) 2012-12-06 2012-12-06 Dgat genes comprising pleckstrin homology domains and use in recombinant microorganisms

Publications (1)

Publication Number Publication Date
BR112015012811A2 true BR112015012811A2 (pt) 2017-11-14

Family

ID=50881337

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015012811A BR112015012811A2 (pt) 2012-12-06 2012-12-06 genes dgat compreendendo domínios de homologia de plecstrina e uso em micro-organismos recombinantes

Country Status (6)

Country Link
US (1) US8835149B2 (pt)
EP (1) EP2929040A4 (pt)
AU (1) AU2012396236B2 (pt)
BR (1) BR112015012811A2 (pt)
CA (1) CA2893926A1 (pt)
WO (1) WO2014088583A1 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6613731B2 (ja) 2015-09-02 2019-12-04 株式会社デンソー トリグリセリド生産性が改良された真核微細藻類遺伝子改変株及びその利用
JP6580912B2 (ja) * 2015-09-11 2019-09-25 花王株式会社 脂質の製造方法
CN108660079B (zh) * 2017-03-31 2021-11-23 财团法人食品工业发展研究所 微芒藻属(micractinium sp.)及其用途
EP3720869A4 (en) 2017-12-08 2021-11-24 Synthetic Genomics, Inc. IMPROVEMENT OF ALGAE LIPID PRODUCTIVITY BY GENETIC MODIFICATION OF A PROTEIN CONTAINING A TPR DOMAIN
CN114258398A (zh) * 2019-06-13 2022-03-29 总医院公司 工程化的人内源性病毒样颗粒及使用其递送至细胞的方法
CN110699334B (zh) * 2019-11-08 2021-07-30 山东禹王生态食业有限公司 一种酰基转移酶及其应用
CN111206023A (zh) * 2020-02-07 2020-05-29 北京大学 一种高效提高微藻甘油三酯含量的代谢工程方法

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5447858A (en) 1984-04-13 1995-09-05 Mycogen Plant Sciences, Inc. Heat shock promoter and gene
US5753475A (en) 1985-01-17 1998-05-19 Calgene, Inc. Methods and compositions for regulated transcription and expression of heterologous genes
US5614395A (en) 1988-03-08 1997-03-25 Ciba-Geigy Corporation Chemically regulatable and anti-pathogenic DNA sequences and uses thereof
US5639952A (en) 1989-01-05 1997-06-17 Mycogen Plant Science, Inc. Dark and light regulated chlorophyll A/B binding protein promoter-regulatory system
US5563328A (en) 1992-08-19 1996-10-08 Board Of Regents, University Of Nebraska-Lincoln Promoters from chlorella virus genes providing for expression of genes in prokaryotic and eukaryotic hosts
US5464758A (en) 1993-06-14 1995-11-07 Gossen; Manfred Tight control of gene expression in eucaryotic cells by tetracycline-responsive promoters
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5661017A (en) 1993-09-14 1997-08-26 Dunahay; Terri Goodman Method to transform algae, materials therefor, and products produced thereby
CZ372297A3 (cs) 1995-05-26 1998-03-18 Zeneca Limited Přepínání genové exprese využívající receptoru pro ekdyson
US6027900A (en) 1996-04-12 2000-02-22 Carnegie Institution Of Washington Methods and tools for transformation of eukaryotic algae
JP2000511050A (ja) 1996-05-31 2000-08-29 メディジーン・アクチェンゲゼルシャフト 機能性分子表面の生成、スクリーニング、および展開のための新規の合成タンパク質の構造の鋳型
US5846774A (en) 1997-03-21 1998-12-08 Bionebraska, Inc. Chlorella virus promoters
BR9915519A (pt) 1998-11-20 2001-07-17 Dow Agrosciences Llc Sequencias reguladoras úteis para a expressão gênica em tecido embrionário de plantas
WO2000062601A1 (en) 1999-04-15 2000-10-26 Northeastern University Agrobacterium-mediated genetic transformation of multicellular marine algae, resultant strains and their products
US20030041353A1 (en) 2001-04-18 2003-02-27 Henry Daniell Mutiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants
CA2483337C (en) 2002-04-23 2015-10-27 The Scripps Research Institute Expression of polypeptides in chloroplasts, and compositions and methods for expressing same
US7135290B2 (en) 2003-04-12 2006-11-14 Solazyme, Inc. Methods and compositions for evolving hydrogenase genes
AU2004256442A1 (en) 2003-07-01 2005-01-20 Biolex, Inc. Chloroplast transformation of duckweed
US7267976B2 (en) 2003-07-02 2007-09-11 E.I. Du Pont De Nemours And Company Acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous yeasts
US7198937B2 (en) 2004-11-04 2007-04-03 E. I. Du Pont De Nemours And Company Mortierella alpina diacylglycerol acyltransferase for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
US7273746B2 (en) 2004-11-04 2007-09-25 E.I. Dupont De Nemours And Company Diacylglycerol acyltransferases for alteration of polyunsaturated fatty acids and oil content in oleaginous organisms
WO2007133558A2 (en) 2006-05-09 2007-11-22 The Scripps Research Institute Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast
US7642405B2 (en) 2006-05-17 2010-01-05 James Weifu Lee Switchable photosystem-II designer algae for photobiological hydrogen production
AU2008237350B2 (en) 2007-04-03 2013-02-07 Tabletop Energy Methods for transforming eukaryotic algae
MX2010006169A (es) 2007-12-04 2010-06-23 Univ Ohio State Res Found Enfoques moleculares para la optimizacion de la produccion de biocombustibles.
ES2610482T3 (es) 2007-12-21 2017-04-27 National Research Council Of Canada Genes de la diacilglicerol aciltransferasa 2 y proteínas codificadas por los mismos procedentes de algas
WO2009124070A1 (en) 2008-03-31 2009-10-08 Kuehnle Agrosystems, Inc. Nuclear based expression of genes for production of biofuels and process co-products in algae
WO2009149470A1 (en) 2008-06-06 2009-12-10 Aurora Biofuels, Inc. Vcp-based vectors for algal cell transformation
US20100255550A1 (en) 2008-09-15 2010-10-07 Board Of Trustees Of Michigan State University Regulatory Factors Controlling Oil Biosynthesis In Microalgae And Their Use
US8394621B2 (en) 2008-10-23 2013-03-12 Matrix Genetrics, LLC Modified photosynthetic microorganisms for producing triglycerides
US8362318B2 (en) 2008-12-18 2013-01-29 Board Of Trustees Of Michigan State University Enzyme directed oil biosynthesis in microalgae
US8835137B2 (en) 2008-12-23 2014-09-16 Matrix Genetics, Llc Modified photosynthetic microorganisms with reduced glycogen and their use in producing carbon-based products
WO2011026008A1 (en) 2009-08-28 2011-03-03 Phycal Llc Biofuel from recombinant oleginous algae using sugar carbon sources
CN102630140A (zh) 2009-09-15 2012-08-08 蓝宝石能源公司 用于转化栅藻和杜氏藻叶绿体基因组的系统
US8980613B2 (en) 2010-04-06 2015-03-17 Matrix Genetics, Llc Modified photosynthetic microorganisms for producing lipids
WO2011156520A2 (en) 2010-06-09 2011-12-15 Green Pacific Biologicals, Inc. Compositions and methods for increasing oil production and secretion
CA2804925A1 (en) 2010-06-25 2011-12-29 Basf Plant Science Company Gmbh Acyltransferases from thraustochytrium species and uses thereof in fatty acid production
EP2635105B1 (en) 2010-11-04 2017-12-20 Ben-Gurion University of The Negev Research and Development Authority Acyl-coa: diacylglycerol acyltransferase 1-like gene (ptdgat1) and uses thereof
US9523096B2 (en) 2010-12-20 2016-12-20 Matrix Genetics, Llc Modified photosynthetic microorganisms for producing lipids
CN102492672A (zh) 2011-11-26 2012-06-13 宁波大学 一种催化二酰甘油酰基化的酶
EP2909304A4 (en) 2012-10-16 2016-09-21 Exxonmobil Res & Eng Co DGAT GENE AND METHOD FOR USE IN TRIGLYCERIDE PRODUCTION IN RECOMBINANT MICROORGANISMS

Also Published As

Publication number Publication date
WO2014088583A1 (en) 2014-06-12
EP2929040A1 (en) 2015-10-14
AU2012396236B2 (en) 2015-10-15
US8835149B2 (en) 2014-09-16
EP2929040A4 (en) 2016-11-09
CA2893926A1 (en) 2014-06-12
AU2012396236A1 (en) 2015-06-25
US20140162329A1 (en) 2014-06-12

Similar Documents

Publication Publication Date Title
BR112015012811A2 (pt) genes dgat compreendendo domínios de homologia de plecstrina e uso em micro-organismos recombinantes
IN2014DN10996A (pt)
BR112014031424A2 (pt) tratamento de células pluripotentes
EP2279243A4 (en) COMBINED CHEMICAL AND GENETIC APPROACHES FOR GENERATING INDUCED PLURIPOTENTER STEM CELLS
BR112016014488A2 (pt) Meio para cultura celular
BR112014007852A2 (pt) nucleosídeos, nucleotídeos e ácidos nucleicos modificados e usos dos mesmos
BR112015012295A8 (pt) Uso de um inibidor de wee1, e, kit para identificar um paciente com câncer
BR112016019940A2 (pt) edição de genoma sem nucleases
BR112013007862A2 (pt) ácidos nucleicos manipulados e métodos de uso dos mesmos.
BR112013023757A2 (pt) método para redução de viscosidade no processo de sacarificação
BR112015030491A8 (pt) método de modular a expressão de um ácido nucleico alvo em uma célula, bem como método de alterar um ácido nucleico de dna alvo em uma célula
GB2509040A (en) Stabilization of resistive memory
BR112016012870A8 (pt) composições e métodos para melhorar a produção de alface
BR112013005634A2 (pt) canal-rodopsina 2 mutante
EA201792402A2 (ru) Молекулы полинуклеотидов для регуляции генов у растений
CO6470854A2 (es) Variantes de celulasa con expresión, actividad y/o estabilidad mejoradas, y uso de las mismas
BR112013009083A2 (pt) anticorpos humanos de oncostatin m e métodos de uso
BR102012019673A8 (pt) Reator tubular
SG10201804952QA (en) Glucosylceramide synthase inhibitors
BR112013026093A2 (pt) mistura de combustível renovável de baixo ponto de escoamento
CA2863600A1 (en) Modulators of acyl-coa lysocardiolipin acyltransferase 1 ( alcat1) and uses thereof
IN2015DN02381A (pt)
BR112014011066A8 (pt) artigo absorvente com elemento de estabilização
MY180183A (en) Methods and compositions for preventing norleucine misincorporation into proteins
BR112015020667A2 (pt) método de preparar inibidores de glucosilceramida sintase

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 7A ANUIDADE.

B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements
B350 Update of information on the portal [chapter 15.35 patent gazette]