SG172220A1 - Production of influenza vaccines - Google Patents
Production of influenza vaccines Download PDFInfo
- Publication number
- SG172220A1 SG172220A1 SG2011044385A SG2011044385A SG172220A1 SG 172220 A1 SG172220 A1 SG 172220A1 SG 2011044385 A SG2011044385 A SG 2011044385A SG 2011044385 A SG2011044385 A SG 2011044385A SG 172220 A1 SG172220 A1 SG 172220A1
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- virus
- vietnam
- genes
- strain
- influenza
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C—CHEMISTRY; METALLURGY
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Landscapes
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| US12296108P | 2008-12-16 | 2008-12-16 | |
| PCT/US2009/068321 WO2010077986A2 (en) | 2008-12-16 | 2009-12-16 | Production of viral vaccine |
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| EP2563389B1 (en) * | 2010-04-30 | 2015-07-08 | Temasek Life Sciences Laboratory Limited | Universal vaccine against h5n1 lineages |
| KR101250005B1 (ko) | 2010-12-31 | 2013-04-03 | 연세대학교 산학협력단 | 바이러스 유전자 조작을 통한 생백신 제조방법 |
| CN103608453B (zh) | 2012-03-02 | 2018-07-24 | 思齐乐 | 流感病毒重配 |
| KR20150110494A (ko) * | 2012-12-03 | 2015-10-02 | 노파르티스 아게 | 재배열 인플루엔자 a 바이러스 |
| SG11201507454XA (en) | 2013-03-13 | 2015-10-29 | Novartis Ag | Influenza b virus reassortment |
| CN104073513B (zh) * | 2013-03-25 | 2018-04-20 | 中国科学院上海巴斯德研究所 | 甲型流感病毒疫苗哺乳动物细胞适应株的获得方法和适应位点 |
| SG11201509265SA (en) * | 2013-05-10 | 2015-12-30 | Novartis Ag | Avoiding narcolepsy risk in influenza vaccines |
| CN105483149A (zh) * | 2015-12-25 | 2016-04-13 | 吉林农业大学 | 广谱型抗禽流感重组乳酸菌及其制备方法 |
| CN105950789A (zh) * | 2016-07-14 | 2016-09-21 | 广西壮族自治区兽医研究所 | 一种鉴定h5亚型aiv、n6亚型aiv和aiv的引物组合及其应用 |
| KR101966346B1 (ko) * | 2017-01-13 | 2019-04-05 | 전북대학교 산학협력단 | 시가 독소 Stx2e를 제조하는 방법 및 이를 포함하는 부종병을 예방하기 위한 백신 조성물 |
| WO2019044636A1 (ja) | 2017-08-28 | 2019-03-07 | 一般財団法人阪大微生物病研究会 | リアソータントインフルエンザウイルスの段階的作出方法 |
| KR102182987B1 (ko) * | 2018-06-27 | 2020-11-27 | 대한민국 | 재조합 인플루엔자 a 바이러스 h5n1주 및 이를 포함하는 고병원성 인플루엔자 a 바이러스 백신 조성물 |
| CN109096377B (zh) * | 2018-09-19 | 2021-03-23 | 天康生物股份有限公司 | 禽流感病毒血凝素抗原及表达其的bhk-21细胞株、制备方法和疫苗 |
| CN109134624A (zh) * | 2018-09-19 | 2019-01-04 | 天康生物股份有限公司 | 禽流感病毒血凝素抗原及其制备方法、应用和禽流感疫苗 |
| KR102182999B1 (ko) * | 2019-01-04 | 2020-11-25 | 대한민국 | 재조합 인플루엔자 A 바이러스 및 이를 포함하는 clade 2.3.4.4B에 속하는 H5 혈청형 인플루엔자 A 바이러스에 대한 백신 조성물 |
| KR102182997B1 (ko) * | 2019-01-04 | 2020-11-26 | 대한민국 | H5N6형 재조합 인플루엔자 A 바이러스 및 이를 포함하는 clade 2.3.4.4D에 속하는 H5 혈청형 인플루엔자 A 바이러스에 대한 백신 조성물 |
| WO2021229270A1 (es) | 2020-05-13 | 2021-11-18 | Laboratorio Avi-Mex, S.A. De C.V. | Vacuna recombinante contra covid-19 en vector viral |
| CN111647610B (zh) * | 2020-06-02 | 2021-09-03 | 扬州大学 | 一种互换ha和ns1缺失基因包装信号的h9n2亚型禽流感病毒及其构建方法和应用 |
| WO2021257469A1 (en) * | 2020-06-15 | 2021-12-23 | Icahn School Of Medicine At Mount Sinai | Immunoassay for the detection of anti-sars-cov-2 spike protein antibody in milk samples |
| CN114524862B (zh) * | 2021-12-16 | 2022-12-27 | 中国农业科学院哈尔滨兽医研究所（中国动物卫生与流行病学中心哈尔滨分中心） | 禽流感(h5+h7)三价dna疫苗的构建及其应用 |
Family Cites Families (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4552758A (en) | 1983-12-20 | 1985-11-12 | St. Jude Children's Research Hospital | Human use of avian-human reassortants as vaccines for influenza A virus |
| CA2017507C (en) | 1989-05-25 | 1996-11-12 | Gary Van Nest | Adjuvant formulation comprising a submicron oil droplet emulsion |
| US6001634A (en) | 1989-08-28 | 1999-12-14 | Palese; Peter | Recombinant negative strand RNA viruses |
| US6022726A (en) | 1992-02-03 | 2000-02-08 | Palese; Peter | Genetically engineered attenuated viruses |
| US7344722B1 (en) | 1993-06-29 | 2008-03-18 | The Regents Of The University Of Michigan | Cold-adapted influenza virus |
| GB9326253D0 (en) | 1993-12-23 | 1994-02-23 | Smithkline Beecham Biolog | Vaccines |
| US6146873A (en) | 1994-11-10 | 2000-11-14 | Baxter Aktiengesellschaft | Production of orthomyxoviruses in monkey kidney cells using protein-free media |
| US6254873B1 (en) | 1995-04-17 | 2001-07-03 | The United States Of America As Represented By The Secretary Of The Army | Inactivated dengue virus vaccine |
| US5840565A (en) | 1995-08-22 | 1998-11-24 | The Regents Of The University Of California | Methods for enhancing the production of viral vaccines in PKR-deficient cell culture |
| JP4837827B2 (ja) | 1998-06-12 | 2011-12-14 | マウント シナイ スクール オブ メディシン | 新規なウイルス増殖方法およびそのためのインターフェロン欠損培養基 |
| DE69937999T2 (de) | 1998-06-12 | 2009-01-29 | Avir Green Hills Biotechnology Research Development Trade Ag | Interferon induzierende genetisch veränderte attenuierte viren |
| AT407958B (de) | 1999-02-11 | 2001-07-25 | Immuno Ag | Inaktivierte influenza-virus-vakzine zur nasalen oder oralen applikation |
| US7521220B2 (en) * | 1999-11-26 | 2009-04-21 | Crucell Holland B.V. | Production of vaccines |
| US6803041B2 (en) | 2001-03-20 | 2004-10-12 | Boehringer Ingelheim Vetmedica, Inc. | Equine herpesvirus vaccine |
| US7465456B2 (en) | 2002-04-26 | 2008-12-16 | Medimmune, Llc | Multi plasmid system for the production of influenza virus |
| DK1499348T3 (en) | 2002-04-26 | 2015-01-05 | Medimmune Llc | PROCEDURE FOR PREPARING INFECTIOUS INFLUENZA B-VIRA IN CELL CULTURE |
| US20060110406A1 (en) | 2003-02-25 | 2006-05-25 | Medimmune Vaccines, Inc. | Refrigerator-temperature stable influenza vaccine compositions |
| EP2481819A1 (en) | 2003-02-25 | 2012-08-01 | MedImmune Vaccines, Inc. | Cross flow filtration in the production of stabilized influenza vaccine compositions |
| US7566458B2 (en) | 2003-06-16 | 2009-07-28 | Medimmune, Llc | Influenza hemagglutinin and neuraminidase variants |
| US7037707B2 (en) | 2003-09-04 | 2006-05-02 | St. Jude Children's Research Hospital | Method for generating influenza viruses and vaccines |
| CN1618956A (zh) * | 2003-11-20 | 2005-05-25 | 中国科学院武汉病毒研究所 | 一种禽流感预防用病毒株及其动物感染模型 |
| CA2551489C (en) | 2003-12-23 | 2013-09-03 | Gregory Duke | Multi plasmid system for the production of influenza virus |
| HUE025727T2 (en) | 2004-05-24 | 2016-04-28 | Medimmune Llc | Multi-plasmid system for producing influenza virus |
| JP4980895B2 (ja) | 2004-05-25 | 2012-07-18 | メディミューン，エルエルシー | インフルエンザ赤血球凝集素およびノイラミニダーゼ変異体 |
| DK2497492T3 (en) | 2004-06-01 | 2018-11-26 | Icahn School Med Mount Sinai | Genetically modified swine influenza virus and its applications |
| EP2769735B1 (en) | 2004-10-06 | 2017-04-19 | MedImmune, LLC | Refrigerator-temperature stable influenza vaccine compositions |
| US7510719B2 (en) | 2004-12-08 | 2009-03-31 | Medimmune, Llc | Methods of producing influenza vaccine compositions |
| EP2471552B1 (en) | 2005-03-08 | 2015-05-06 | MedImmune, LLC | Reassortant influenza viruses |
| EP1945778B1 (en) | 2005-10-17 | 2012-12-26 | MedImmune, LLC | Methods of increasing influenza virus replication |
| US20080014217A1 (en) | 2006-07-17 | 2008-01-17 | Emmanuel Jules Hanon | Influenza vaccine |
| WO2008133701A1 (en) | 2006-07-21 | 2008-11-06 | Medimmune, Llc. | Methods and compositions for increasing replication capacity of an influenza virus |
| CN104140958A (zh) | 2006-08-09 | 2014-11-12 | 米迪缪尼有限公司 | 流感血凝素和神经氨酸酶变体 |
| CA2666980A1 (en) | 2006-09-26 | 2008-04-03 | Id Biomedical Corporation Of Quebec | Device and methods of inactivating influenza virus and adventitious agents with ultraviolet light |
| JP2010512748A (ja) | 2006-12-15 | 2010-04-30 | シェーリング−プラウ・リミテッド | インフルエンザウイルスを培養物中で複製するための方法 |
| EP2674486A1 (en) | 2007-06-18 | 2013-12-18 | MedImmune, LLC | Influenza B viruses having alterations in the hemaglutinin polypeptide |
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| WO2010077986A3 (en) | 2010-10-07 |
| US9884105B2 (en) | 2018-02-06 |
| JP2012511914A (ja) | 2012-05-31 |
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