SG11201903018WA - Early post-transfection isolation of cells (epic) for biologics production - Google Patents
Early post-transfection isolation of cells (epic) for biologics productionInfo
- Publication number
- SG11201903018WA SG11201903018WA SG11201903018WA SG11201903018WA SG11201903018WA SG 11201903018W A SG11201903018W A SG 11201903018WA SG 11201903018W A SG11201903018W A SG 11201903018WA SG 11201903018W A SG11201903018W A SG 11201903018WA SG 11201903018W A SG11201903018W A SG 11201903018WA
- Authority
- SG
- Singapore
- Prior art keywords
- international
- cells
- rule
- bridgewater
- sanofi
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- 229960000074 biopharmaceutical Drugs 0.000 title abstract 2
- 238000002955 isolation Methods 0.000 title abstract 2
- 238000001890 transfection Methods 0.000 title abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 4
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 4
- 238000000034 method Methods 0.000 abstract 2
- 238000001943 fluorescence-activated cell sorting Methods 0.000 abstract 1
- 238000002826 magnetic-activated cell sorting Methods 0.000 abstract 1
- 230000008520 organization Effects 0.000 abstract 1
- 238000010187 selection method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70592—CD52
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/0081—Purging biological preparations of unwanted cells
- C12N5/0087—Purging against subsets of blood cells, e.g. purging alloreactive T cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Optical investigation techniques, e.g. flow cytometry
- G01N15/149—Optical investigation techniques, e.g. flow cytometry specially adapted for sorting particles, e.g. by their size or optical properties
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Toxicology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Tropical Medicine & Parasitology (AREA)
- Hematology (AREA)
- Dispersion Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Epoxy Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662405392P | 2016-10-07 | 2016-10-07 | |
| PCT/US2017/055583 WO2018067964A1 (en) | 2016-10-07 | 2017-10-06 | Early post-transfection isolation of cells (epic) for biologics production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201903018WA true SG11201903018WA (en) | 2019-05-30 |
Family
ID=60302441
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201903018WA SG11201903018WA (en) | 2016-10-07 | 2017-10-06 | Early post-transfection isolation of cells (epic) for biologics production |
| SG10202103545PA SG10202103545PA (en) | 2016-10-07 | 2017-10-06 | Early post-transfection isolation of cells (epic) for biologics production |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10202103545PA SG10202103545PA (en) | 2016-10-07 | 2017-10-06 | Early post-transfection isolation of cells (epic) for biologics production |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US20180119192A1 (zh) |
| EP (1) | EP3523440A1 (zh) |
| CN (1) | CN110050065B (zh) |
| AU (2) | AU2017341028B2 (zh) |
| CA (1) | CA3039598A1 (zh) |
| MX (1) | MX2019004013A (zh) |
| SG (2) | SG11201903018WA (zh) |
| TW (2) | TW202346598A (zh) |
| WO (1) | WO2018067964A1 (zh) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3359665A2 (en) | 2015-10-09 | 2018-08-15 | Genzyme Corporation | Improved flare (flow cytometry attenuated reporter expression) technology for rapid bulk sorting |
| MX2019004013A (es) | 2016-10-07 | 2019-08-14 | Genzyme Corp | Aislamiento temprano de celulas despues de la transfeccion (epic) para la produccion de productos biologicos. |
Family Cites Families (43)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
| US4937190A (en) | 1987-10-15 | 1990-06-26 | Wisconsin Alumni Research Foundation | Translation enhancer |
| US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
| JPH05501054A (ja) | 1989-07-24 | 1993-03-04 | セラジェン・インコーポレーテッド | 内部翻訳開始の防止 |
| US5648267A (en) | 1992-11-13 | 1997-07-15 | Idec Pharmaceuticals Corporation | Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same |
| WO1994026087A2 (en) | 1993-05-14 | 1994-11-24 | Connor Kim C O | Recombinant protein production and insect cell culture and process |
| US5429746A (en) | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
| US7252989B1 (en) | 1994-04-04 | 2007-08-07 | Board Of Regents, The University Of Texas System | Adenovirus supervector system |
| US5955349A (en) | 1996-08-26 | 1999-09-21 | Zymogenetics, Inc. | Compositions and methods for producing heterologous polypeptides in Pichia methanolica |
| US5736383A (en) | 1996-08-26 | 1998-04-07 | Zymogenetics, Inc. | Preparation of Pichia methanolica auxotrophic mutants |
| AU6667798A (en) | 1997-02-25 | 1998-09-09 | Q.B.I. Enterprises Ltd. | Ires sequences with high translational efficiency and expression vectors containing the sequence |
| US7001733B1 (en) | 1998-05-12 | 2006-02-21 | Rigel Pharmaceuticals, Inc. | Methods and compositions for screening for modulations of IgE synthesis, secretion and switch rearrangement |
| US6258559B1 (en) | 1999-03-22 | 2001-07-10 | Zymogenetics, Inc. | Method for producing proteins in transformed Pichia |
| US20050005310A1 (en) | 1999-07-12 | 2005-01-06 | Genentech, Inc. | Expression vectors and methods |
| ES2257303T3 (es) | 1999-07-12 | 2006-08-01 | Genentech, Inc. | Vectores de expresion y procedimientos. |
| WO2001057212A1 (en) | 2000-02-02 | 2001-08-09 | Iconix Pharmaceuticals, Inc. | Rapid, parallel identification of cell lines |
| WO2002070710A1 (en) | 2001-03-01 | 2002-09-12 | University Of California | Method to identify ires elements |
| DE10143237A1 (de) | 2001-09-04 | 2003-03-20 | Icon Genetics Ag | Herstellung künstlicher interner ribosomaler Eingangsstellenelemente (Ires-Elemente) |
| DE10143238A1 (de) | 2001-09-04 | 2003-03-20 | Icon Genetics Ag | Identifizierung eukaryotischer interner Ribosomen-Eingangsstellen (IRES)-Elemente |
| WO2003099996A2 (en) | 2002-05-22 | 2003-12-04 | Biogen Idec Ma Inc. | Detection of secreted polypeptides |
| US7119187B2 (en) | 2002-07-09 | 2006-10-10 | National Health Research Institutes | Internal ribosome entry site of the labial gene for protein expression |
| GB0216648D0 (en) | 2002-07-18 | 2002-08-28 | Lonza Biologics Plc | Method of expressing recombinant protein in CHO cells |
| US20040180378A1 (en) | 2002-07-19 | 2004-09-16 | Eileen Tozer | Fluorescent proteins, nucleic acids encoding them and methods for making and using them |
| US7384744B2 (en) | 2002-11-29 | 2008-06-10 | Boehringer Ingelheim Pharma Gmbh & Co., Kg | Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products |
| US7344886B2 (en) * | 2002-11-29 | 2008-03-18 | Boehringer Ingelheim Pharma Gmbh & Co., Kg | Neomycin-phosphotransferase-genes and methods for the selection of recombinant cells producing high levels of a desired gene product |
| WO2004060910A2 (en) | 2002-12-16 | 2004-07-22 | Wayne State University | Bioactive peptides and unique ires elements from myelin proteolipid protein plp/dm20 |
| PL2332972T3 (pl) | 2003-06-24 | 2018-06-29 | Genzyme Corporation | Nowe promotory beta-aktynowe i RPS21 i ich zastosowania |
| US7776584B2 (en) | 2003-08-01 | 2010-08-17 | Genetix Limited | Animal cell colony picking apparatus and method |
| JPWO2005094886A1 (ja) | 2004-03-31 | 2008-02-14 | キリンファーマ株式会社 | Ｇｐｉアンカー蛋白質アゴニストによる調節性ｔ細胞分化誘導・増殖方法およびそのための医薬組成物 |
| US7078179B2 (en) | 2004-05-04 | 2006-07-18 | Newlink Genetics Corporation | Selectable gene marker system based on expression of N-acetyllactosaminide 3-α galactosyltransferase |
| AU2005300503B2 (en) | 2004-11-08 | 2010-12-16 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
| SI1809750T1 (sl) | 2004-11-08 | 2012-08-31 | Chromagenics Bv | Izbira gostiteljskih celic, ki imajo visok nivo izraĹľanja proteina |
| US20080248468A1 (en) * | 2004-12-14 | 2008-10-09 | Manfred Kubbies | Method for Improved Selection of Rnai Transfectants |
| EP2010668A4 (en) * | 2006-04-10 | 2009-04-29 | Univ California | SYSTEMS AND METHODS FOR EFFICIENT COLLECTION OF UNIQUE CELLS AND COLONIES OF CELLS AND RAPID FORMATION OF STABLE TRANSFECTORS |
| US20090239235A1 (en) | 2006-09-20 | 2009-09-24 | Demaria Christine | Facs- and Reporter Protein-Based System for High Throughput Development of Therapeutic Proteins |
| CA2679393A1 (en) | 2007-03-05 | 2008-09-12 | Newsouth Innovations Pty Limited | Methods for detecting and modulating the sensitivity of tumour cells to anti-mitotic agents |
| DK2443239T3 (en) * | 2009-06-15 | 2016-02-15 | Cellagenics B V | New stringent selection markers |
| US9534246B2 (en) | 2010-07-01 | 2017-01-03 | Glaxo Group Limited | Method for selecting high producing cell lines |
| AU2013243951A1 (en) | 2012-04-02 | 2014-10-30 | Moderna Therapeutics, Inc. | Modified polynucleotides for the production of secreted proteins |
| US20160017319A1 (en) | 2013-03-11 | 2016-01-21 | Audrey Nommay | Method of screening cell clones |
| EP3359665A2 (en) | 2015-10-09 | 2018-08-15 | Genzyme Corporation | Improved flare (flow cytometry attenuated reporter expression) technology for rapid bulk sorting |
| MX2019004013A (es) | 2016-10-07 | 2019-08-14 | Genzyme Corp | Aislamiento temprano de celulas despues de la transfeccion (epic) para la produccion de productos biologicos. |
-
2017
- 2017-10-06 MX MX2019004013A patent/MX2019004013A/es unknown
- 2017-10-06 WO PCT/US2017/055583 patent/WO2018067964A1/en unknown
- 2017-10-06 SG SG11201903018WA patent/SG11201903018WA/en unknown
- 2017-10-06 CN CN201780075590.6A patent/CN110050065B/zh active Active
- 2017-10-06 EP EP17797462.3A patent/EP3523440A1/en active Pending
- 2017-10-06 TW TW112104729A patent/TW202346598A/zh unknown
- 2017-10-06 AU AU2017341028A patent/AU2017341028B2/en active Active
- 2017-10-06 CA CA3039598A patent/CA3039598A1/en active Pending
- 2017-10-06 TW TW106134586A patent/TWI795373B/zh active
- 2017-10-06 US US15/727,272 patent/US20180119192A1/en not_active Abandoned
- 2017-10-06 SG SG10202103545PA patent/SG10202103545PA/en unknown
-
2019
- 2019-11-14 US US16/683,837 patent/US11685943B2/en active Active
-
2023
- 2023-05-10 US US18/315,332 patent/US20240026410A1/en active Pending
-
2024
- 2024-03-21 AU AU2024201853A patent/AU2024201853A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3523440A1 (en) | 2019-08-14 |
| US20240026410A1 (en) | 2024-01-25 |
| TWI795373B (zh) | 2023-03-11 |
| SG10202103545PA (en) | 2021-05-28 |
| AU2017341028A1 (en) | 2019-05-23 |
| AU2017341028B2 (en) | 2023-12-21 |
| AU2024201853A1 (en) | 2024-04-11 |
| WO2018067964A1 (en) | 2018-04-12 |
| CN110050065A (zh) | 2019-07-23 |
| CA3039598A1 (en) | 2018-04-12 |
| MX2019004013A (es) | 2019-08-14 |
| CN110050065B (zh) | 2024-05-07 |
| TW201827598A (zh) | 2018-08-01 |
| TW202346598A (zh) | 2023-12-01 |
| US11685943B2 (en) | 2023-06-27 |
| US20180119192A1 (en) | 2018-05-03 |
| US20200299743A1 (en) | 2020-09-24 |
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