SG11201906213UA - Modulating expression of polypeptides via new gene switch expression systems - Google Patents

Modulating expression of polypeptides via new gene switch expression systems

Info

Publication number
SG11201906213UA
SG11201906213UA SG11201906213UA SG11201906213UA SG11201906213UA SG 11201906213U A SG11201906213U A SG 11201906213UA SG 11201906213U A SG11201906213U A SG 11201906213UA SG 11201906213U A SG11201906213U A SG 11201906213UA SG 11201906213U A SG11201906213U A SG 11201906213UA
Authority
SG
Singapore
Prior art keywords
international
rule
expression
polypeptides
blacksburg
Prior art date
Application number
SG11201906213UA
Inventor
Rutul R Shah
Thomas D Reed
Cheryl G Bolinger
Original Assignee
Intrexon Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Intrexon Corp filed Critical Intrexon Corp
Publication of SG11201906213UA publication Critical patent/SG11201906213UA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/461—Cellular immunotherapy characterised by the cell type used
    • A61K39/4611—T-cells, e.g. tumor infiltrating lymphocytes [TIL], lymphokine-activated killer cells [LAK] or regulatory T cells [Treg]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/463—Cellular immunotherapy characterised by recombinant expression
    • A61K39/4631—Chimeric Antigen Receptors [CAR]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/463—Cellular immunotherapy characterised by recombinant expression
    • A61K39/4635—Cytokines
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643—Vertebrate antigens
    • A61K39/4644—Cancer antigens
    • A61K39/464402—Receptors, cell surface antigens or cell surface determinants
    • A61K39/464403—Receptors for growth factors
    • A61K39/464404—Epidermal growth factor receptors [EGFR]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/46—Cellular immunotherapy
    • A61K39/464—Cellular immunotherapy characterised by the antigen targeted or presented
    • A61K39/4643—Vertebrate antigens
    • A61K39/4644—Cancer antigens
    • A61K39/464402—Receptors, cell surface antigens or cell surface determinants
    • A61K39/464411—Immunoglobulin superfamily
    • A61K39/464412—CD19 or B4
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/54—Interleukins [IL]
    • C07K14/5443—IL-15
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/7051—T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K39/46
    • A61K2239/10—Indexing codes associated with cellular immunotherapy of group A61K39/46 characterized by the structure of the chimeric antigen receptor [CAR]
    • A61K2239/23—On/off switch
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K39/46
    • A61K2239/38—Indexing codes associated with cellular immunotherapy of group A61K39/46 characterised by the dose, timing or administration schedule
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K39/46
    • A61K2239/46—Indexing codes associated with cellular immunotherapy of group A61K39/46 characterised by the cancer treated
    • A61K2239/47—Brain; Nervous system
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/90—Vectors containing a transposable element

Abstract

O C (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (43) International Publication Date 19 July 2018 (19.07.2018) WIP0 1 PCT omit °nolo oil! nit ow (10) International Publication Number WO 2018/132494 Al (51) International Patent Classification: Cl 2N 15/10 (2006.01) G01N 33/68 (2006.01) Cl 2N 15/63 (2006.01) (21) International Application Number: PCT/US2018/013196 (22) International Filing Date: 10 January 2018 (10.01.2018) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/444,775 10 January 2017 (10.01.2017) US 62/464,958 28 February 2017 (28.02.2017) US (71) Applicant: INTREXON CORPORATION [US/US]; 1750 Kraft Drive, Blacksburg, Virginia 24060 (US). (72) Inventors: SHAH, Rutul R.; 1750 Kraft Dr., Blacksburg, Virginia 24060 (US). REED, Thomas D.; 1750 Kraft Dr., Blacksburg, Virginia 24060 (US). BOLINGER, Cheryl G.; 1750 Kraft Dr., Blacksburg, Virginia 24060 (US). (74) Agent: MATA, David; Wilson Sonsini Goodrich & Rosati, 650 Page Mill Road, Palo Alto, California 94304 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). (54) Title: MODULATING EXPRESSION OF POLYPEPTIDES VIA NEW GENE SWITCH EXPRESSION SYSTEMS CDS CD4 Ali Veledimex (nM) FIG. 13C (57) : Disclosed herein are polynucleotides encoding ligand-inducible gene switch polypeptides, and systems comprising gene switch polypeptides for modulating the expression of a heterologous gene and an interleukin in a host cell. The compositions, methods and systems described herein facilitate ligand dependent expression of polypeptides including but not limited to cytokines and antigen binding polypeptides. [Continued on next page] WO 2018/132494 Al MIDEDIMOMOIDEIREEMOMOIIIMMIEMOHMEHOIMIE Declarations under Rule 4.17: as to applicant's entitlement to apply for and be granted a patent (Rule 4.17(11)) as to the applicant's entitlement to claim the priority of the earlier application (Rule 4.17(iii)) Published: with international search report (Art. 21(3)) before the expiration of the time limit for amending the claims and to be republished in the event of receipt of amendments (Rule 48.2(h)) with sequence listing part of description (Rule 5.2(a))
SG11201906213UA 2017-01-10 2018-01-10 Modulating expression of polypeptides via new gene switch expression systems SG11201906213UA (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762444775P 2017-01-10 2017-01-10
US201762464958P 2017-02-28 2017-02-28
PCT/US2018/013196 WO2018132494A1 (en) 2017-01-10 2018-01-10 Modulating expression of polypeptides via new gene switch expression systems

Publications (1)

Publication Number Publication Date
SG11201906213UA true SG11201906213UA (en) 2019-08-27

Family

ID=61094600

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201906213UA SG11201906213UA (en) 2017-01-10 2018-01-10 Modulating expression of polypeptides via new gene switch expression systems

Country Status (11)

Country Link
US (2) US11692194B2 (en)
EP (1) EP3568474A1 (en)
JP (2) JP7288401B2 (en)
KR (1) KR20190102259A (en)
CN (1) CN110462040A (en)
AU (1) AU2018207281A1 (en)
CA (1) CA3049652A1 (en)
IL (2) IL310711A (en)
SG (1) SG11201906213UA (en)
WO (1) WO2018132494A1 (en)
ZA (1) ZA201904623B (en)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3105335B1 (en) * 2014-02-14 2019-10-02 Board Of Regents, The University Of Texas System Chimeric antigen receptors and methods of making
CA3047313A1 (en) 2016-12-16 2018-06-21 B-Mogen Biotechnologies, Inc. Enhanced hat family transposon-mediated gene transfer and associated compositions, systems, and methods
US11278570B2 (en) 2016-12-16 2022-03-22 B-Mogen Biotechnologies, Inc. Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods
KR20200096758A (en) * 2017-10-18 2020-08-13 인트렉손 코포레이션 Polypeptide composition comprising a spacer
WO2019160956A1 (en) * 2018-02-13 2019-08-22 Novartis Ag Chimeric antigen receptor therapy in combination with il-15r and il15
JP2021527427A (en) 2018-06-21 2021-10-14 ビー−モーゲン・バイオテクノロジーズ，インコーポレーテッド Transposon-mediated gene transfer and related compositions, systems, and methods of the enhanced hAT family
SG11202100205UA (en) * 2018-07-10 2021-02-25 Precigen Inc Ror-1 specific chimeric antigen receptors and uses thereof
CN110832071A (en) * 2018-09-14 2020-02-21 上海原能细胞医学技术有限公司 Cells comprising an immunomodulator on their surface and uses thereof
WO2020052645A1 (en) * 2018-09-14 2020-03-19 上海原能细胞医学技术有限公司 Cell containing immunomodulator on surface and use thereof
US20230045048A1 (en) * 2018-12-14 2023-02-09 Proviva Therapeutics (Hong Kong) Limited Il-15 compositions and methods of use thereof
CA3134539A1 (en) * 2019-04-05 2020-10-08 Nektar Therapeutics Method for enhancing cellular immunotherapy
KR20220005541A (en) * 2019-04-30 2022-01-13 아타라 바이오테라퓨틱스 인크. Antigen specific CD19-targeted CAR-T cells
EP3753566A1 (en) * 2019-06-21 2020-12-23 Medizinische Hochschule Hannover All-in one viral vector for car and therapeutic effector molecule
AU2020345943A1 (en) * 2019-09-10 2022-03-31 Obsidian Therapeutics, Inc. CA2-IL15 fusion proteins for tunable regulation
CN113646435A (en) * 2019-12-09 2021-11-12 南克维斯特公司 Screening of cell clones expressing polygene transgenes by antibiotic-independent positive selection
EP4072596A4 (en) * 2019-12-12 2024-03-06 Senti Biosciences Inc Method and compositions for regulated armoring of cells
EP4107272A2 (en) * 2020-02-19 2022-12-28 Wuxi Biologics Ireland Limited Enhanced expression system and methods of use thereof
EP4247850A1 (en) 2020-11-20 2023-09-27 Simcere Innovation, Inc. Armed dual car-t compositions and methods for cancer immunotherapy
CA3203531A1 (en) * 2020-12-30 2022-07-07 Simon Olivares Recombinant vectors comprising polycistronic expression cassettes and methods of use thereof
WO2022235811A2 (en) * 2021-05-04 2022-11-10 Editas Medicine, Inc. Engineered cells for therapy
US20230265457A1 (en) * 2021-10-25 2023-08-24 Genvivo, Inc. Compositions and methods for therapeutic delivery

Family Cites Families (118)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4985461A (en) 1985-10-21 1991-01-15 Rohm And Haas Company Insecticidal N'-substituted-N,N'-diacylhydrazines
US5117057A (en) 1985-10-21 1992-05-26 Rohm And Haas Company Insecticidal N' substituted-N-N'-disubstituted-hydrazines
GB8601597D0 (en) 1986-01-23 1986-02-26 Wilson R H Nucleotide sequences
FR2593630B1 (en) 1986-01-27 1988-03-18 Maurice Francois DRAIN RESISTANCE ACTIVE MATRIX DISPLAY SCREEN AND METHODS OF MAKING SAME
US5225443A (en) 1986-05-01 1993-07-06 Rohm And Haas Company Insecticidal N'-substituted-N'-substituted N,N'-diacylhydrazines
US6218525B1 (en) * 1988-02-25 2001-04-17 The General Hospital Corporation Nucleic acid encoding CD28
US5858358A (en) 1992-04-07 1999-01-12 The United States Of America As Represented By The Secretary Of The Navy Methods for selectively stimulating proliferation of T cells
US6905680B2 (en) 1988-11-23 2005-06-14 Genetics Institute, Inc. Methods of treating HIV infected subjects
US6352694B1 (en) 1994-06-03 2002-03-05 Genetics Institute, Inc. Methods for inducing a population of T cells to proliferate using agents which recognize TCR/CD3 and ligands which stimulate an accessory molecule on the surface of the T cells
US6534055B1 (en) 1988-11-23 2003-03-18 Genetics Institute, Inc. Methods for selectively stimulating proliferation of T cells
US20040049014A1 (en) * 1988-12-28 2004-03-11 Protein Design Labs, Inc. Humanized immunoglobulins
US5585362A (en) 1989-08-22 1996-12-17 The Regents Of The University Of Michigan Adenovirus vectors for gene therapy
US5283173A (en) 1990-01-24 1994-02-01 The Research Foundation Of State University Of New York System to detect protein-protein interactions
WO1991014695A1 (en) 1990-03-22 1991-10-03 The Salk Institute For Biological Studies Insect retinoid receptor compositions and methods
CA2043775A1 (en) 1990-06-14 1991-12-15 Dat P. Le Dibenzoylakylcyanohydrazines
EP0557459B1 (en) 1990-11-13 1997-10-22 Immunex Corporation Bifunctional selectable fusion genes
IL100643A (en) 1991-01-25 1996-10-31 Nippon Kayaku Kk Hydrazine derivatives and pesticidal compositions comprising such derivatives as effective component
US5530028A (en) 1992-11-23 1996-06-25 Rohm And Haas Company Insecticidal N'-substituted-N,N'-diacylhydrazines
US6013836A (en) 1992-02-28 2000-01-11 Rohm And Haas Company Insecticidal N'-substituted-N,N'-disubstitutedhydrazines
US5350674A (en) 1992-09-04 1994-09-27 Becton, Dickinson And Company Intrinsic factor - horse peroxidase conjugates and a method for increasing the stability thereof
EP0804590A1 (en) 1993-05-21 1997-11-05 Targeted Genetics Corporation Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene
US7175843B2 (en) 1994-06-03 2007-02-13 Genetics Institute, Llc Methods for selectively stimulating proliferation of T cells
ES2237764T3 (en) 1995-03-03 2005-08-01 Syngenta Participations Ag CONTROL OF GENE EXPRESSION IN PLANTS BY TRANSACTIVATION MEDIATED BY A RECEIVER IN THE PRESENCE OF A CHEMICAL BINDING.
AU720201B2 (en) 1995-03-08 2000-05-25 Scripps Research Institute, The Antigen presenting system and methods for activation of T-cells
US5785682A (en) * 1995-03-22 1998-07-28 Abbott Laboratories Pre-filled syringe drug delivery system
US7067318B2 (en) 1995-06-07 2006-06-27 The Regents Of The University Of Michigan Methods for transfecting T cells
US6692964B1 (en) 1995-05-04 2004-02-17 The United States Of America As Represented By The Secretary Of The Navy Methods for transfecting T cells
US5885827A (en) 1996-01-23 1999-03-23 The Regents Of The Universtiy Of California Eukaryotic high rate mutagenesis system
EP0910652B1 (en) 1996-04-05 2014-10-08 The Salk Institute For Biological Studies Hormone-mediated methods for modulating expression of exogenous genes in mammalian systems, and products related thereto
AU723355B2 (en) 1996-05-23 2000-08-24 Scripps Research Institute, The MHC class II antigen presenting systems and methods for activating CD4+ T cells
WO1998040510A1 (en) 1997-03-11 1998-09-17 Regents Of The University Of Minnesota Dna-based transposon system for the introduction of nucleic acid into dna of a cell
WO1999002683A1 (en) 1997-07-10 1999-01-21 The Salk Institute For Biological Studies Modified lepidopteran receptors and hybrid multifunctional proteins for use in regulation of transgene expression
US6333318B1 (en) 1998-05-14 2001-12-25 The Salk Institute For Biological Studies Formulations useful for modulating expression of exogenous genes in mammalian systems, and products related thereto
US6790662B1 (en) 1999-03-12 2004-09-14 Ortho-Mcneil Pharmaceutical, Inc. Method of isolating CD8+ cells, and related hybridoma cells antibodies and polypeptides
CA2375490A1 (en) * 1999-06-18 2000-12-28 Ariad Gene Therapeutics, Inc. Chimeric oca-b transcription factors
WO2001029058A1 (en) 1999-10-15 2001-04-26 University Of Massachusetts Rna interference pathway genes as tools for targeted genetic interference
AU1100201A (en) 1999-10-28 2001-05-08 Board Of Trustees Of The Leland Stanford Junior University Methods of in vivo gene transfer using a sleeping beauty transposon system
US6326193B1 (en) 1999-11-05 2001-12-04 Cambria Biosciences, Llc Insect control agent
US6797514B2 (en) 2000-02-24 2004-09-28 Xcyte Therapies, Inc. Simultaneous stimulation and concentration of cells
WO2001062895A2 (en) 2000-02-24 2001-08-30 Xcyte Therapies, Inc. Simultaneous stimulation and concentration of cells
US6867041B2 (en) 2000-02-24 2005-03-15 Xcyte Therapies, Inc. Simultaneous stimulation and concentration of cells
US7572631B2 (en) 2000-02-24 2009-08-11 Invitrogen Corporation Activation and expansion of T cells
CA2404253C (en) 2000-03-22 2014-05-13 Rohm And Haas Company Novel ecdysone receptor-based inducible gene expression system
US20040033600A1 (en) 2001-03-21 2004-02-19 Palli Subba Reddy Ecdysone receptor-based inducible gene expression system
WO2001096584A2 (en) 2000-06-12 2001-12-20 Akkadix Corporation Materials and methods for the control of nematodes
US8105825B2 (en) 2000-10-03 2012-01-31 Intrexon Corporation Multiple inducible gene regulation system
DK1534738T3 (en) 2001-02-20 2012-10-01 Intrexon Corp Novel substitution mutant receptors and their use in an inducible gene expression system based on a nuclear receptor
US20040071671A1 (en) 2001-02-20 2004-04-15 Leturcq Didier J. Cell therapy method for the treatment of tumors
CA2441444C (en) 2001-02-20 2013-09-03 Rheogene Holdings, Inc. Novel ecdysone receptor/invertebrate retinoid x receptor-based inducible gene expression system
WO2002066612A2 (en) 2001-02-20 2002-08-29 Rheogene, Inc. Novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system
CA2438119C (en) 2001-02-20 2014-12-16 Rheogene Holdings, Inc. Chimeric retinoid x receptors and their use in a novel ecdysone receptor-based inducible gene expression system
JP2005507247A (en) 2001-09-26 2005-03-17 レオジーン・ホールディングス，インコーポレーテッド Whitefly ecdysone receptor nucleic acids, polypeptides, and uses thereof
MXPA04002810A (en) 2001-09-26 2005-06-06 Rheogene Holdings Inc Leafhopper ecdysone receptor nucleic acids, polypeptides, and uses thereof.
US7745140B2 (en) 2002-01-03 2010-06-29 The Trustees Of The University Of Pennsylvania Activation and expansion of T-cells using an engineered multivalent signaling platform as a research tool
AU2003231048A1 (en) 2002-04-22 2003-11-03 Regents Of The University Of Minnesota Transposon system and methods of use
US7375093B2 (en) 2002-07-05 2008-05-20 Intrexon Corporation Ketone ligands for modulating the expression of exogenous genes via an ecdysone receptor complex
WO2004033685A1 (en) 2002-10-09 2004-04-22 Avidex Ltd Single chain recombinant t cell receptors
US7304161B2 (en) 2003-02-10 2007-12-04 Intrexon Corporation Diaclhydrazine ligands for modulating the expression of exogenous genes in mammalian systems via an ecdysone receptor complex
US7304162B2 (en) 2003-02-21 2007-12-04 Intrexon Corporation Oxadiazoline ligands for modulating the expression of exogenous genes via an ecdysone receptor complex
US7456315B2 (en) 2003-02-28 2008-11-25 Intrexon Corporation Bioavailable diacylhydrazine ligands for modulating the expression of exogenous genes via an ecdysone receptor complex
US8802643B1 (en) 2003-05-30 2014-08-12 University Of South Florida Method for the treatment of malignancies
US7985739B2 (en) 2003-06-04 2011-07-26 The Board Of Trustees Of The Leland Stanford Junior University Enhanced sleeping beauty transposon system and methods for using the same
NZ570709A (en) 2003-06-13 2010-04-30 Univ Pennsylvania Nucleic acid sequences encoding and compositions comprising IgE signal peptide and/or IL-15 and methods for using the same
US7935510B2 (en) 2004-04-30 2011-05-03 Intrexon Corporation Mutant receptors and their use in a nuclear receptor-based inducible gene expression system
WO2005118788A2 (en) 2004-05-27 2005-12-15 The Trustees Of The University Of Pennsylvania Novel artificial antigen presenting cells and uses therefor
ATE475669T1 (en) 2004-06-29 2010-08-15 Immunocore Ltd CELLS EXPRESSING A MODIFIED T-CELL RECEPTOR
US7847064B2 (en) * 2004-07-09 2010-12-07 Donald Danforth Plant Science Center Methods and compositions for regulating gene expression in plant cells
EP1809321B1 (en) 2004-10-08 2012-03-14 Government of the United States of America, Represented by the Secretary, Department of Health and Human Services Adoptive immunotherapy with enhanced t lymphocyte survival
PL1899364T3 (en) 2005-05-17 2020-08-24 University Of Connecticut Compositions and methods for immunomodulation in an organism
EP1777294A1 (en) * 2005-10-20 2007-04-25 Institut National De La Sante Et De La Recherche Medicale (Inserm) IL-15Ralpha sushi domain as a selective and potent enhancer of IL-15 action through IL-15Rbeta/gamma, and hyperagonist (IL15Ralpha sushi -IL15) fusion proteins
ES2579762T3 (en) 2006-03-01 2016-08-16 Janssen Pharmaceutica N.V. Cancer treatment by combining lympho - reducing agent with CLTs and cytokines
NZ574093A (en) * 2006-06-12 2011-03-31 Symphogen As Pan-cell surface receptor- specific therapeutics
ES2603418T3 (en) 2006-10-04 2017-02-27 Janssen Pharmaceutica Nv Preparation of inactivated artificial antigen presenting cells and their use in cell therapies
EP2126105A4 (en) 2007-02-20 2010-11-03 Anaptysbio Inc Somatic hypermutation systems
AU2008233051B2 (en) 2007-03-30 2014-04-10 Memorial Sloan-Kettering Cancer Center Constitutive expression of costimulatory ligands on adoptively transferred T lymphocytes
ES2466916T3 (en) 2007-06-27 2014-06-11 Admune Therapeutics Llc IL-15 and IL-15R alpha complexes and uses thereof
JP5726522B2 (en) 2007-07-04 2015-06-03 マックス−デルブリュック−ツェントルム フューア モレキュラーレ メディツィン Transposon system-a hyperactive variant of the sleeping beauty transposase protein
EP2205249B1 (en) 2007-09-28 2018-11-07 Intrexon Corporation Therapeutic gene-switch constructs and bioreactors for the expression of biotherapeutic molecules, and uses thereof
JP2010539993A (en) * 2007-10-08 2010-12-24 イントレキソン コーポレーション Use for the treatment of genetically engineered dendritic cells and cancer
MX2010009996A (en) * 2008-03-14 2010-10-20 Intrexon Corp Steroidal ligands and their use in gene switch modulation.
CA2739902A1 (en) 2008-10-08 2010-04-15 Intrexon Corporation Engineered cells expressing multiple immunomodulators and uses thereof
CA2760446C (en) * 2009-04-30 2018-01-02 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Inducible interleukin-12
JP5508625B2 (en) 2009-12-14 2014-06-04 トヨタ自動車株式会社 Vehicle control apparatus and vehicle control method
TWI688395B (en) 2010-03-23 2020-03-21 英翠克頌公司 Vectors conditionally expressing therapeutic proteins, host cells comprising the vectors, and uses thereof
US9331524B1 (en) 2010-07-03 2016-05-03 Best Energy Reduction Technologies, Llc Method, system and apparatus for monitoring and measuring power usage
WO2012122025A2 (en) * 2011-03-04 2012-09-13 Intrexon Corporation Vectors conditionally expressing protein
EA028804B1 (en) * 2011-03-25 2018-01-31 Гленмарк Фармасьютикалс С.А. Hetero-dimeric immunoglobulins
EP2694549B1 (en) * 2011-04-08 2018-08-15 The United States of America, as represented by The Secretary, Department of Health and Human Services Anti-epidermal growth factor receptor variant iii chimeric antigen receptors and use of same for the treatment of cancer
US20130071414A1 (en) 2011-04-27 2013-03-21 Gianpietro Dotti Engineered cd19-specific t lymphocytes that coexpress il-15 and an inducible caspase-9 based suicide gene for the treatment of b-cell malignancies
WO2014159415A1 (en) 2013-03-12 2014-10-02 Bio-Rad Laboratories, Inc. Colloidal coomassie stain
KR102238226B1 (en) 2013-05-14 2021-04-09 보드 오브 리전츠, 더 유니버시티 오브 텍사스 시스템 Human application of engineered chimeric antigen receptor (car) t-cells
EP3004168A4 (en) 2013-05-24 2017-03-01 Board of Regents, The University of Texas System Chimeric antigen receptor-targeting monoclonal antibodies
JP6720075B2 (en) * 2013-05-31 2020-07-08 メルク・シャープ・アンド・ドーム・コーポレーションＭｅｒｃｋ Ｓｈａｒｐ ＆ Ｄｏｈｍｅ Ｃｏｒｐ． Combination therapy for cancer
KR20160087903A (en) * 2013-11-29 2016-07-22 유씨비 바이오파마 에스피알엘 Synthetic promoters for cho cells, methods of producing synthetic promoters using transcription factor binding site modules
EP3083666A4 (en) 2013-12-18 2017-09-20 Intrexon Corporation Single chain il-12 nucleic acids, polypeptids, and uses thereof
DK3105246T3 (en) * 2014-02-10 2021-06-14 Merck Patent Gmbh Targeted TGF-beta inhibition
US9814215B2 (en) 2014-03-11 2017-11-14 Little Big Cat, Inc. Pet toy with telescoping wand with metallic wire
EP3593812A3 (en) 2014-03-15 2020-05-27 Novartis AG Treatment of cancer using chimeric antigen receptor
CA2947466A1 (en) * 2014-05-01 2015-11-05 University Of Washington In vivo gene engineering with adenoviral vectors
DK3143134T3 (en) 2014-05-15 2021-01-04 Nat Univ Singapore Modified, natural killer cells and their uses
CN106536564B (en) * 2014-06-02 2021-08-31 美国卫生和人力服务部 Chimeric antigen receptor targeting CD-19
US11030531B2 (en) 2014-06-06 2021-06-08 Trustees Of Boston University DNA recombinase circuits for logical control of gene expression
US10335460B2 (en) 2014-07-29 2019-07-02 Novartis Ag IL-15 and IL-15Rα heterodimer dose escalation regimens for treating conditions
TWI751102B (en) 2014-08-28 2022-01-01 美商奇諾治療有限公司 Antibodies and chimeric antigen receptors specific for cd19
WO2016048903A1 (en) 2014-09-22 2016-03-31 Intrexon Corporation Improved therapeutic control of heterodimeric and single chain forms of interleukin-12
CA2964785A1 (en) 2014-11-05 2016-05-12 Board Of Regents, The University Of Texas System Gene modified immune effector cells and engineered cells for expansion of immune effector cells
US9888673B2 (en) 2014-12-10 2018-02-13 Regents Of The University Of Minnesota Genetically modified cells, tissues, and organs for treating disease
WO2016126608A1 (en) 2015-02-02 2016-08-11 Novartis Ag Car-expressing cells against multiple tumor antigens and uses thereof
JP6775515B2 (en) 2015-03-11 2020-10-28 ボード・オブ・リージエンツ，ザ・ユニバーシテイ・オブ・テキサス・システム Transposase polypeptide and its use
CN113502270A (en) * 2015-06-10 2021-10-15 南克维斯特公司 Modified NK-92cells for the treatment of cancer
AU2016326575A1 (en) 2015-09-25 2018-04-12 Altor Bioscience Corporation Interleukin-15 superagonist significantly enhances graft-versus-tumor activity
WO2017062953A1 (en) 2015-10-10 2017-04-13 Intrexon Corporation Improved therapeutic control of proteolytically sensitive, destabilized forms of interleukin-12
US11020429B2 (en) * 2015-11-05 2021-06-01 Juno Therapeutics, Inc. Vectors and genetically engineered immune cells expressing metabolic pathway modulators and uses in adoptive cell therapy
EP3387010A1 (en) 2015-11-11 2018-10-17 Intrexon Corporation Compositions and methods for expression of multiple biologically active polypeptides from a single vector for treatment of cardiac conditions and other pathologies
CN106967685B (en) * 2016-01-13 2020-06-02 北京马力喏生物科技有限公司 Transgenic lymphocytes co-expressing anti-EGFRvIII chimeric antigen receptor and immune checkpoint inhibitory molecules and uses thereof
WO2017177063A1 (en) 2016-04-06 2017-10-12 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Use of heterodimeric il-15 in adoptive cell transfer
US9877990B2 (en) * 2016-04-08 2018-01-30 Krystal Biotech, Inc. Compositions and methods for the treatment of wounds, disorders, and diseases of the skin
IL270990B2 (en) 2017-06-07 2024-02-01 Precigen Inc Expression of novel cell tags

Also Published As

Publication number Publication date
AU2018207281A1 (en) 2019-07-18
JP2020513777A (en) 2020-05-21
IL267865A (en) 2019-09-26
IL310711A (en) 2024-04-01
JP2023065438A (en) 2023-05-12
CA3049652A1 (en) 2018-07-19
ZA201904623B (en) 2023-12-20
EP3568474A1 (en) 2019-11-20
US20180291384A1 (en) 2018-10-11
US11692194B2 (en) 2023-07-04
CN110462040A (en) 2019-11-15
US11946054B2 (en) 2024-04-02
KR20190102259A (en) 2019-09-03
JP7288401B2 (en) 2023-06-07
US20200283778A1 (en) 2020-09-10
IL267865B1 (en) 2024-03-01
WO2018132494A1 (en) 2018-07-19

Similar Documents

Publication Publication Date Title
SG11201906213UA (en) Modulating expression of polypeptides via new gene switch expression systems
SG11201907713WA (en) Nucleic acid based data storage
SG11201901563UA (en) De novo synthesized nucleic acid libraries
SG11201906264YA (en) Improved serum albumin binders
SG11201909724RA (en) Systems and processes for conversion of crude oil
SG11201908796XA (en) Improved antigen binding receptors
SG11201808196UA (en) Neoantigens and methods of their use
SG11201811025VA (en) Systems and methods for automated annotation and screening of biological sequences
SG11201810600WA (en) Fxr (nr1h4) modulating compounds
SG11201906341XA (en) Improved serum albumin binders
SG11201908319WA (en) Methods and systems for printing biological material
SG11201908784TA (en) Improved antigen binding receptor formats
SG11201901221YA (en) Methods for detecting aav
SG11201407508RA (en) Encoding and decoding based on blending of sequences of samples along time
SG11201909011PA (en) Niraparib compositions
SG11201906468TA (en) Chimeric antigen receptors against axl or ror2 and methods of use thereof
SG11201807881VA (en) Compositions and methods for making antibodies based on use of an expression-enhancing locus
SG11201906142QA (en) Conversion of crude oil to aromatic and olefinic petrochemicals
SG11201407818PA (en) High-capacity storage of digital information in dna
SG11201900746RA (en) Engineered antibodies and other fc-domain containing molecules with enhanced agonism and effector functions
SG11201407343XA (en) Subfamily e simian adenoviruses a1302, a1320, a1331 and a1337 and uses thereof
SG11201906152RA (en) Conversion of crude oil to aromatic and olefinic petrochemicals
SG11201907671XA (en) Systems and methods for cracking hydrocarbon streams such as crude oils utilizing catalysts which include zeolite mixtures
SG11201908456VA (en) Linker units and molecular constructs comprising same
SG11201809789SA (en) Dna monoclonal antibodies targeting checkpoint molecules