SG11201809242VA - Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor - Google Patents
Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used thereforInfo
- Publication number
- SG11201809242VA SG11201809242VA SG11201809242VA SG11201809242VA SG11201809242VA SG 11201809242V A SG11201809242V A SG 11201809242VA SG 11201809242V A SG11201809242V A SG 11201809242VA SG 11201809242V A SG11201809242V A SG 11201809242VA SG 11201809242V A SG11201809242V A SG 11201809242VA
- Authority
- SG
- Singapore
- Prior art keywords
- used therefor
- molecular complex
- genome sequence
- introduction efficiency
- modification technique
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 238000012986 modification Methods 0.000 title abstract 2
- 230000004048 modification Effects 0.000 title abstract 2
- 230000035772 mutation Effects 0.000 title abstract 2
- 239000002773 nucleotide Substances 0.000 abstract 5
- 125000003729 nucleotide group Chemical group 0.000 abstract 5
- 108020004414 DNA Proteins 0.000 abstract 3
- 102000053602 DNA Human genes 0.000 abstract 3
- 238000012258 culturing Methods 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 150000007523 nucleic acids Chemical group 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
- C12Y305/04—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
- C12Y305/04005—Cytidine deaminase (3.5.4.5)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2016085631 | 2016-04-21 | ||
| PCT/JP2017/016105 WO2017183724A1 (ja) | 2016-04-21 | 2017-04-21 | ゲノム配列改変技術における変異導入効率の向上方法、及びそれに用いる分子複合体 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201809242VA true SG11201809242VA (en) | 2018-11-29 |
Family
ID=60116870
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201809242VA SG11201809242VA (en) | 2016-04-21 | 2017-04-21 | Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US20200377910A1 (de) |
| EP (1) | EP3447139B1 (de) |
| JP (1) | JP7001272B2 (de) |
| KR (1) | KR102116200B1 (de) |
| CN (2) | CN109312329B (de) |
| BR (1) | BR112018071376A2 (de) |
| CA (1) | CA3021281C (de) |
| DK (1) | DK3447139T3 (de) |
| ES (1) | ES2919961T3 (de) |
| SG (1) | SG11201809242VA (de) |
| WO (1) | WO2017183724A1 (de) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK3382019T3 (da) | 2015-11-27 | 2022-05-30 | Univ Kobe Nat Univ Corp | Fremgangsmåde til omdannelse af enkimet plantegenomsekvens, hvori nukleinsyrebase i målrettet DNA-sekvens specifikt omdannes, og molekylært kompleks anvendt deri |
| KR20200103769A (ko) * | 2018-01-23 | 2020-09-02 | 기초과학연구원 | 연장된 단일 가이드 rna 및 그 용도 |
| CN110607320B (zh) * | 2018-11-23 | 2023-05-12 | 电子科技大学 | 一种植物基因组定向碱基编辑骨架载体及其应用 |
| WO2020241869A1 (ja) * | 2019-05-30 | 2020-12-03 | 国立大学法人東京大学 | ２種の核酸塩基変換酵素が融合されたＣａｓタンパク質を利用したゲノム編集システム |
| US20220333133A1 (en) | 2019-09-03 | 2022-10-20 | Voyager Therapeutics, Inc. | Vectorized editing of nucleic acids to correct overt mutations |
| IL292117A (en) * | 2019-10-17 | 2022-06-01 | Pairwise Plants Services Inc | Variants of cas12a nuclei and methods of production and use thereof |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2003251286B2 (en) | 2002-01-23 | 2007-08-16 | The University Of Utah Research Foundation | Targeted chromosomal mutagenesis using zinc finger nucleases |
| JP2013128413A (ja) | 2010-03-11 | 2013-07-04 | Kyushu Univ | Ｐｐｒモチーフを利用したｒｎａ結合性蛋白質の改変方法 |
| JP2015527889A (ja) * | 2012-07-25 | 2015-09-24 | ザ ブロード インスティテュート， インコーポレイテッド | 誘導可能なｄｎａ結合タンパク質およびゲノム撹乱ツール、ならびにそれらの適用 |
| CN111471675A (zh) * | 2014-03-05 | 2020-07-31 | 国立大学法人神户大学 | 特异性转变靶向dna序列的核酸碱基的基因组序列的修饰方法、及其使用的分子复合体 |
| JP6153180B2 (ja) * | 2014-11-04 | 2017-06-28 | 国立大学法人神戸大学 | 脱塩基反応により標的化したｄｎａ配列に特異的に変異を導入する、ゲノム配列の改変方法、並びにそれに用いる分子複合体 |
| WO2017009073A1 (de) * | 2015-07-13 | 2017-01-19 | Huf Hülsbeck & Fürst Gmbh & Co. Kg | Türaussengriff für ein fahrzeug |
| JP6780860B2 (ja) * | 2015-09-09 | 2020-11-04 | 国立大学法人神戸大学 | 標的化したｄｎａ配列の核酸塩基を特異的に変換するゲノム配列の改変方法及びそれに用いる分子複合体 |
| JP6664693B2 (ja) * | 2015-09-09 | 2020-03-13 | 国立大学法人神戸大学 | 標的化したｄｎａ配列の核酸塩基を特異的に変換する、グラム陽性菌のゲノム配列の変換方法、及びそれに用いる分子複合体 |
| IL294014B2 (en) * | 2015-10-23 | 2024-07-01 | Harvard College | Nucleobase editors and their uses |
| DK3382019T3 (da) * | 2015-11-27 | 2022-05-30 | Univ Kobe Nat Univ Corp | Fremgangsmåde til omdannelse af enkimet plantegenomsekvens, hvori nukleinsyrebase i målrettet DNA-sekvens specifikt omdannes, og molekylært kompleks anvendt deri |
-
2017
- 2017-04-21 DK DK17786061.6T patent/DK3447139T3/da active
- 2017-04-21 WO PCT/JP2017/016105 patent/WO2017183724A1/ja active Application Filing
- 2017-04-21 JP JP2018513234A patent/JP7001272B2/ja active Active
- 2017-04-21 EP EP17786061.6A patent/EP3447139B1/de active Active
- 2017-04-21 CN CN201780038364.0A patent/CN109312329B/zh active Active
- 2017-04-21 US US16/094,587 patent/US20200377910A1/en active Pending
- 2017-04-21 ES ES17786061T patent/ES2919961T3/es active Active
- 2017-04-21 BR BR112018071376-7A patent/BR112018071376A2/pt active Search and Examination
- 2017-04-21 CA CA3021281A patent/CA3021281C/en active Active
- 2017-04-21 KR KR1020187032638A patent/KR102116200B1/ko active IP Right Grant
- 2017-04-21 CN CN202311476665.5A patent/CN117925730A/zh active Pending
- 2017-04-21 SG SG11201809242VA patent/SG11201809242VA/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20200377910A1 (en) | 2020-12-03 |
| EP3447139B1 (de) | 2022-06-08 |
| CN109312329A (zh) | 2019-02-05 |
| CN109312329B (zh) | 2023-11-21 |
| ES2919961T3 (es) | 2022-07-29 |
| CA3021281C (en) | 2021-07-27 |
| BR112018071376A2 (pt) | 2019-04-24 |
| JPWO2017183724A1 (ja) | 2019-02-28 |
| EP3447139A1 (de) | 2019-02-27 |
| CN117925730A (zh) | 2024-04-26 |
| EP3447139A4 (de) | 2019-10-02 |
| JP7001272B2 (ja) | 2022-01-19 |
| KR20180132862A (ko) | 2018-12-12 |
| CA3021281A1 (en) | 2017-10-26 |
| WO2017183724A1 (ja) | 2017-10-26 |
| DK3447139T3 (da) | 2022-07-04 |
| KR102116200B1 (ko) | 2020-05-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| SG11201809242VA (en) | Method for increasing mutation introduction efficiency in genome sequence modification technique, and molecular complex to be used therefor | |
| PH12017502281A1 (en) | Thermostable cas9 nucleases | |
| MX2024007924A (es) | Edicion genomica multiplexada. | |
| EA201991442A1 (ru) | ТЕРМОСТАБИЛЬНЫЕ НУКЛЕАЗЫ Cas9 | |
| GB2569734A (en) | RNA-guided nucleic acid modifying enzymes and methods of use thereof | |
| NZ723399A (en) | Methods and compositions for dna profiling | |
| NZ705675A (en) | Engineered transgene integration platform (etip) for gene targeting and trait stacking | |
| EP3382019A4 (de) | Verfahren zur umwandlung einer monokotpflanzengenomsequenz, in der eine nukleinsäurebasis in gezielter dna-sequenz spezifisch umgewandelt wird, und darin verwendeter molekularkomplex | |
| FI3604532T3 (fi) | Uusia crispr-entsyymejä ja järjestelmiä | |
| MX2018000729A (es) | Métodos para amplificar las secuencias de ácido nucleico. | |
| MX367170B (es) | Enriquecimiento de la clasificacion de las celulas activadas por fluorescencia (facs) para generar plantas. | |
| EA201791343A1 (ru) | Композиции и способы для конструирования вирусного генома in vitro | |
| NZ723570A (en) | Compositions and methods for quantifying a nucleic acid sequence in a sample | |
| NZ705742A (en) | Fad2 performance loci and corresponding target site specific binding proteins capable of inducing targeted breaks | |
| NZ600733A (en) | Engineered landing pads for gene targeting in plants | |
| MX2024002327A (es) | Sistemas y metodos para direccion con arn guia puntual (arngp) de adn endogeno y fuente. | |
| MX2021000209A (es) | Metodos y productos para transfeccion de celulas. | |
| RU2016104056A (ru) | Мультиплексная геномная инженерия, направляемая рнк | |
| RU2016133286A (ru) | Способы мутагенеза | |
| GEP20217251B (en) | Methods and compositions for rna-directed target dna modification and for rna-directed modulation of transcription | |
| EP4286523A3 (de) | Verfahren zur verbesserung der effizienz von homologie-gerichteter reparatur (hdr) im zellulären genom | |
| WO2008051530A3 (en) | Polymerase enzymes and reagents for enhanced nucleic acid sequencing | |
| EA201070229A1 (ru) | Нуклеотидные последовательности msca1, влияющие на мужскую фертильность растений, и способ их применения | |
| MY184632A (en) | A method of identifying parentage in freshwater prawn macrobrachium rosenbergii | |
| WO2010080782A3 (en) | Random homozygous gene perburbation (rhgp) with thermal assymetric interlaced (tail)-pcr |