SG11201407917PA - Highly efficient method for establishing artificial pluripotent stem cell - Google Patents

Highly efficient method for establishing artificial pluripotent stem cell

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Publication number
SG11201407917PA
SG11201407917PA SG11201407917PA SG11201407917PA SG11201407917PA SG 11201407917P A SG11201407917P A SG 11201407917PA SG 11201407917P A SG11201407917P A SG 11201407917PA SG 11201407917P A SG11201407917P A SG 11201407917PA SG 11201407917P A SG11201407917P A SG 11201407917PA
Authority
SG
Singapore
Prior art keywords
episomal vector
ebna
kyoto
cell
ips cell
Prior art date
Application number
SG11201407917PA
Other languages
English (en)
Inventor
Shinya Yamanaka
Keisuke Okita
Original Assignee
Univ Kyoto
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Kyoto filed Critical Univ Kyoto
Publication of SG11201407917PA publication Critical patent/SG11201407917PA/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1135—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against oncogenes or tumor suppressor genes
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/14—Type of nucleic acid interfering N.A.
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/13—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells
    • C12N2506/1307—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from connective tissue cells, from mesenchymal cells from adult fibroblasts
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    • C12N2710/00011—Details
    • C12N2710/00022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Oncology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Transplantation (AREA)
  • Virology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
SG11201407917PA 2012-05-23 2013-05-23 Highly efficient method for establishing artificial pluripotent stem cell SG11201407917PA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261650694P 2012-05-23 2012-05-23
PCT/JP2013/064409 WO2013176233A1 (ja) 2012-05-23 2013-05-23 効率的な人工多能性幹細胞の樹立方法

Publications (1)

Publication Number Publication Date
SG11201407917PA true SG11201407917PA (en) 2015-01-29

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SG11201407917PA SG11201407917PA (en) 2012-05-23 2013-05-23 Highly efficient method for establishing artificial pluripotent stem cell

Country Status (11)

Country Link
US (1) US10519425B2 (zh)
EP (1) EP2853592B1 (zh)
JP (1) JP6381442B2 (zh)
KR (1) KR102066761B1 (zh)
CN (1) CN104471060B (zh)
AU (1) AU2013264708B2 (zh)
CA (1) CA2874259C (zh)
ES (1) ES2725827T3 (zh)
HK (1) HK1208492A1 (zh)
SG (1) SG11201407917PA (zh)
WO (1) WO2013176233A1 (zh)

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EP2853592B1 (en) 2012-05-23 2019-02-20 Kyoto University Highly efficient method for establishing artificial pluripotent stem cell
WO2015006725A2 (en) 2013-07-12 2015-01-15 Cedars-Sinai Medical Center Generation of induced pluripotent stem cells from normal human mammary epithelial cells
CN103923920B (zh) * 2014-04-04 2016-09-21 清华大学 一种增强人类细胞中非整合性基因表达的方法
WO2016008982A1 (en) * 2014-07-16 2016-01-21 Fundación Pública Andaluza Progreso Y Salud Methods for generating induced pluripotent stem cells
EP3402496A4 (en) * 2016-01-12 2019-06-19 Lonza Walkersville, Inc. METHODS AND VECTORS FOR PRODUCING INDUCED STEM CELLS NOT CONTAINING THE VECTOR
KR102312123B1 (ko) 2016-04-22 2021-10-13 고쿠리츠 다이가쿠 호진 교토 다이가쿠 도파민 생산 신경전구세포의 제조방법
CN106086071B (zh) * 2016-06-13 2019-08-09 广州市搏克生物技术有限公司 一种非病毒iPSCs诱导方法及其诱导组合物、试剂盒及其获得的iPSCs
CN105861447B (zh) * 2016-06-13 2017-12-19 广州市搏克生物技术有限公司 一种非病毒iPSCs诱导组合物及其试剂盒
US10221395B2 (en) * 2016-06-16 2019-03-05 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
US11572545B2 (en) 2016-06-16 2023-02-07 Cedars-Sinai Medical Center Efficient method for reprogramming blood to induced pluripotent stem cells
CN106244558B (zh) * 2016-08-31 2020-01-21 四川新生命干细胞科技股份有限公司 一种人单个核细胞重编程为诱导多能干细胞的方法
JP2018082639A (ja) * 2016-11-21 2018-05-31 学校法人千葉工業大学 体細胞から人工多能性幹細胞を製造する方法及び人工多能性幹細胞を未分化のまま培養するための培地
US20200277627A1 (en) * 2017-09-13 2020-09-03 Biontech Rna Pharmaceuticals Gmbh Rna replicon for reprogramming somatic cells
EP3818158A1 (en) * 2018-07-02 2021-05-12 Shi-Lung Lin In-vitro induction of adult stem cell expansion and derivation
CN109913494A (zh) * 2019-02-12 2019-06-21 北京呈诺医学科技有限公司 一种新的ips细胞的诱导方法
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CN114645023B (zh) * 2022-05-18 2022-09-20 上海爱萨尔生物科技有限公司 将外周血单个核细胞重编程为诱导多能干细胞的系统和方法

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CN104471060B (zh) 2021-11-05
US10519425B2 (en) 2019-12-31
HK1208492A1 (zh) 2016-03-04
EP2853592B1 (en) 2019-02-20
US20150175973A1 (en) 2015-06-25
CA2874259A1 (en) 2013-11-28
CN104471060A (zh) 2015-03-25
AU2013264708A1 (en) 2015-01-15
JPWO2013176233A1 (ja) 2016-01-14
EP2853592A4 (en) 2016-01-13
ES2725827T3 (es) 2019-09-27
EP2853592A1 (en) 2015-04-01
CA2874259C (en) 2021-02-09
AU2013264708B2 (en) 2019-03-07
KR20150014512A (ko) 2015-02-06
JP6381442B2 (ja) 2018-08-29
KR102066761B1 (ko) 2020-01-15
WO2013176233A1 (ja) 2013-11-28

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