SE432949B - Forfarande och reagens for bestemning av urinsyra - Google Patents
Forfarande och reagens for bestemning av urinsyraInfo
- Publication number
- SE432949B SE432949B SE7803056A SE7803056A SE432949B SE 432949 B SE432949 B SE 432949B SE 7803056 A SE7803056 A SE 7803056A SE 7803056 A SE7803056 A SE 7803056A SE 432949 B SE432949 B SE 432949B
- Authority
- SE
- Sweden
- Prior art keywords
- liter
- acid
- nad
- lower alkyl
- group
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 25
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 title claims description 18
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 title claims description 18
- 229940116269 uric acid Drugs 0.000 title claims description 18
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 16
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 claims description 33
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 18
- 239000000654 additive Substances 0.000 claims description 15
- OFOBLEOULBTSOW-UHFFFAOYSA-N Malonic acid Chemical compound OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims description 14
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 claims description 14
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 claims description 14
- WFKAJVHLWXSISD-UHFFFAOYSA-N isobutyramide Chemical compound CC(C)C(N)=O WFKAJVHLWXSISD-UHFFFAOYSA-N 0.000 claims description 13
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 9
- 125000005907 alkyl ester group Chemical group 0.000 claims description 9
- 125000000217 alkyl group Chemical group 0.000 claims description 9
- 102000005369 Aldehyde Dehydrogenase Human genes 0.000 claims description 8
- 108020002663 Aldehyde Dehydrogenase Proteins 0.000 claims description 8
- 102000016938 Catalase Human genes 0.000 claims description 7
- 108010053835 Catalase Proteins 0.000 claims description 7
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Natural products NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 7
- 230000000996 additive effect Effects 0.000 claims description 7
- 150000001875 compounds Chemical class 0.000 claims description 7
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 claims description 7
- 108010092464 Urate Oxidase Proteins 0.000 claims description 6
- 229940047889 isobutyramide Drugs 0.000 claims description 6
- SIOXPEMLGUPBBT-UHFFFAOYSA-N picolinic acid Chemical compound OC(=O)C1=CC=CC=N1 SIOXPEMLGUPBBT-UHFFFAOYSA-N 0.000 claims description 6
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 5
- 125000005843 halogen group Chemical group 0.000 claims description 5
- 150000001408 amides Chemical class 0.000 claims description 4
- MGFYIUFZLHCRTH-UHFFFAOYSA-N nitrilotriacetic acid Chemical compound OC(=O)CN(CC(O)=O)CC(O)=O MGFYIUFZLHCRTH-UHFFFAOYSA-N 0.000 claims description 4
- NOIXNOMHHWGUTG-UHFFFAOYSA-N 2-[[4-[4-pyridin-4-yl-1-(2,2,2-trifluoroethyl)pyrazol-3-yl]phenoxy]methyl]quinoline Chemical compound C=1C=C(OCC=2N=C3C=CC=CC3=CC=2)C=CC=1C1=NN(CC(F)(F)F)C=C1C1=CC=NC=C1 NOIXNOMHHWGUTG-UHFFFAOYSA-N 0.000 claims description 3
- 239000012620 biological material Substances 0.000 claims description 3
- 150000003217 pyrazoles Chemical class 0.000 claims description 3
- 229910019142 PO4 Inorganic materials 0.000 claims description 2
- 239000008139 complexing agent Substances 0.000 claims description 2
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
- 150000003222 pyridines Chemical class 0.000 claims description 2
- 229950006238 nadide Drugs 0.000 claims 4
- 241001122767 Theaceae Species 0.000 claims 1
- 235000006408 oxalic acid Nutrition 0.000 claims 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 1
- 210000002966 serum Anatomy 0.000 description 33
- 238000006243 chemical reaction Methods 0.000 description 24
- 230000008033 biological extinction Effects 0.000 description 11
- WTKZEGDFNFYCGP-UHFFFAOYSA-N Pyrazole Chemical compound C=1C=NNC=1 WTKZEGDFNFYCGP-UHFFFAOYSA-N 0.000 description 9
- XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 9
- 239000000523 sample Substances 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 4
- 229940039748 oxalate Drugs 0.000 description 4
- 239000005515 coenzyme Substances 0.000 description 3
- 208000017169 kidney disease Diseases 0.000 description 3
- 210000004185 liver Anatomy 0.000 description 3
- -1 monoethyl ester Chemical class 0.000 description 3
- KPWDGTGXUYRARH-UHFFFAOYSA-N 2,2,2-trichloroethanol Chemical compound OCC(Cl)(Cl)Cl KPWDGTGXUYRARH-UHFFFAOYSA-N 0.000 description 2
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 238000003149 assay kit Methods 0.000 description 2
- 230000002860 competitive effect Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 125000001424 substituent group Chemical group 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- DFUAIALZXKLUQU-UHFFFAOYSA-N 4,5-dibromo-1h-pyrazole Chemical compound BrC=1C=NNC=1Br DFUAIALZXKLUQU-UHFFFAOYSA-N 0.000 description 1
- WVGCPEDBFHEHEZ-UHFFFAOYSA-N 4-bromo-1h-pyrazole Chemical compound BrC=1C=NNC=1 WVGCPEDBFHEHEZ-UHFFFAOYSA-N 0.000 description 1
- BADSZRMNXWLUKO-UHFFFAOYSA-N 4-chloro-1h-pyrazole Chemical compound ClC=1C=NNC=1 BADSZRMNXWLUKO-UHFFFAOYSA-N 0.000 description 1
- IHNXHUNMFYXQCG-UHFFFAOYSA-N 4-ethyl-1h-pyrazole Chemical compound CCC=1C=NNC=1 IHNXHUNMFYXQCG-UHFFFAOYSA-N 0.000 description 1
- LLNQWPTUJJYTTE-UHFFFAOYSA-N 4-iodopyrazole Chemical compound IC=1C=NNC=1 LLNQWPTUJJYTTE-UHFFFAOYSA-N 0.000 description 1
- YAXKXMVDJJMGRY-UHFFFAOYSA-N 4-propyl-1h-pyrazole Chemical compound CCCC=1C=NNC=1 YAXKXMVDJJMGRY-UHFFFAOYSA-N 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical group [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- WKBOTKDWSSQWDR-UHFFFAOYSA-N Bromine atom Chemical compound [Br] WKBOTKDWSSQWDR-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 201000005569 Gout Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 101100381510 Mus musculus Bcl10 gene Proteins 0.000 description 1
- ZYVXHFWBYUDDBM-UHFFFAOYSA-N N-methylnicotinamide Chemical compound CNC(=O)C1=CC=CN=C1 ZYVXHFWBYUDDBM-UHFFFAOYSA-N 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 150000001447 alkali salts Chemical class 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- 229910052794 bromium Inorganic materials 0.000 description 1
- 125000004432 carbon atom Chemical group C* 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000010580 coupled enzyme reaction Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000001177 diphosphate Substances 0.000 description 1
- XPPKVPWEQAFLFU-UHFFFAOYSA-J diphosphate(4-) Chemical compound [O-]P([O-])(=O)OP([O-])([O-])=O XPPKVPWEQAFLFU-UHFFFAOYSA-J 0.000 description 1
- 235000011180 diphosphates Nutrition 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- RIKMMFOAQPJVMX-UHFFFAOYSA-N fomepizole Chemical compound CC=1C=NNC=1 RIKMMFOAQPJVMX-UHFFFAOYSA-N 0.000 description 1
- 229960004285 fomepizole Drugs 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 description 1
- 150000003891 oxalate salts Chemical class 0.000 description 1
- 235000015277 pork Nutrition 0.000 description 1
- 238000007430 reference method Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- ZNCPFRVNHGOPAG-UHFFFAOYSA-L sodium oxalate Chemical compound [Na+].[Na+].[O-]C(=O)C([O-])=O ZNCPFRVNHGOPAG-UHFFFAOYSA-L 0.000 description 1
- 229940039790 sodium oxalate Drugs 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
- 229940075420 xanthine Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/62—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving uric acid
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2718588A DE2718588C3 (de) | 1977-04-26 | 1977-04-26 | Verfahren und Reagens zur Bestimmung von Harnsäure |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| SE7803056L SE7803056L (sv) | 1978-10-27 |
| SE432949B true SE432949B (sv) | 1984-04-30 |
Family
ID=6007336
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE7803056A SE432949B (sv) | 1977-04-26 | 1978-03-16 | Forfarande och reagens for bestemning av urinsyra |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US4247630A (xx) |
| JP (1) | JPS53135390A (xx) |
| AT (1) | AT354642B (xx) |
| AU (1) | AU511846B2 (xx) |
| BE (1) | BE866414A (xx) |
| CA (1) | CA1098013A (xx) |
| CH (1) | CH643661A5 (xx) |
| DE (1) | DE2718588C3 (xx) |
| DK (1) | DK149476C (xx) |
| FR (1) | FR2389135A1 (xx) |
| GB (1) | GB1578176A (xx) |
| IT (1) | IT1095369B (xx) |
| NL (1) | NL179145C (xx) |
| SE (1) | SE432949B (xx) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6031472B2 (ja) * | 1978-12-14 | 1985-07-22 | 協和醗酵工業株式会社 | 酸性ウリカ−ゼ |
| DE3025170A1 (de) * | 1980-07-03 | 1982-01-28 | C.H. Boehringer Sohn, 6507 Ingelheim | Verfahren und reagenz zur bestimmung von harnsaeure |
| DE3743405A1 (de) * | 1987-05-14 | 1988-11-24 | Boehringer Mannheim Gmbh | Verfahren zur bestimmung von fructosamin |
| IT1247946B (it) * | 1991-05-17 | 1995-01-05 | Instrumentation Lab Srl | Stabilizzazione in forma liquida dell'enzima urato ossidasi |
| US5265301A (en) * | 1992-11-02 | 1993-11-30 | Lawrence Irwin F | Drain cleaning apparatus |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3411887A (en) * | 1964-06-15 | 1968-11-19 | Miles Lab | Diagnostic composition |
| US3335069A (en) * | 1964-12-14 | 1967-08-08 | Miles Lab | Composition and method for determining uric acid |
| US3536448A (en) * | 1967-07-28 | 1970-10-27 | Miles Lab | Uric acid detection |
| DE2237940C3 (de) * | 1972-08-02 | 1980-12-11 | Boehringer Mannheim Gmbh, 6800 Mannheim | Verfahren zur quantitativen Bestimmung von Harnsäure |
| JPS53711B2 (xx) * | 1973-01-16 | 1978-01-11 | ||
| JPS5013356A (xx) * | 1973-06-08 | 1975-02-12 | ||
| US3956069A (en) * | 1974-04-29 | 1976-05-11 | Abbott Laboratories | Enzymatic assays for glucose, creatine phosphokinase or plasma ammonia |
| JPS5123294A (en) * | 1974-08-19 | 1976-02-24 | Seishin Seiyaku Kk | Nyosanno anteikaho |
| DE2450726C3 (de) * | 1974-10-25 | 1981-02-19 | Rainer Prof. Dr.Med. Haeckel | Verfahren zur enzymatischen quantitativen Bestimmung von Wasserstoffperoxid |
-
1977
- 1977-04-26 DE DE2718588A patent/DE2718588C3/de not_active Expired
-
1978
- 1978-01-31 AT AT65778A patent/AT354642B/de not_active IP Right Cessation
- 1978-02-03 CA CA296,266A patent/CA1098013A/en not_active Expired
- 1978-03-02 AU AU33786/78A patent/AU511846B2/en not_active Expired
- 1978-03-16 SE SE7803056A patent/SE432949B/sv not_active IP Right Cessation
- 1978-03-24 IT IT7821633A patent/IT1095369B/it active
- 1978-03-30 NL NLAANVRAGE7803366,A patent/NL179145C/xx not_active IP Right Cessation
- 1978-03-31 US US05/892,360 patent/US4247630A/en not_active Expired - Lifetime
- 1978-03-31 FR FR7809688A patent/FR2389135A1/fr active Granted
- 1978-04-20 JP JP4713878A patent/JPS53135390A/ja active Granted
- 1978-04-20 DK DK172678A patent/DK149476C/da active IP Right Grant
- 1978-04-21 CH CH434578A patent/CH643661A5/de not_active IP Right Cessation
- 1978-04-21 GB GB15854/78A patent/GB1578176A/en not_active Expired
- 1978-04-26 BE BE187138A patent/BE866414A/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| SE7803056L (sv) | 1978-10-27 |
| DK172678A (da) | 1978-10-27 |
| ATA65778A (de) | 1979-06-15 |
| JPS5643240B2 (xx) | 1981-10-09 |
| CA1098013A (en) | 1981-03-24 |
| DK149476B (da) | 1986-06-23 |
| GB1578176A (en) | 1980-11-05 |
| NL179145B (nl) | 1986-02-17 |
| US4247630A (en) | 1981-01-27 |
| NL179145C (nl) | 1986-07-16 |
| JPS53135390A (en) | 1978-11-25 |
| FR2389135A1 (fr) | 1978-11-24 |
| AT354642B (de) | 1979-01-25 |
| DE2718588A1 (de) | 1978-11-09 |
| BE866414A (fr) | 1978-10-26 |
| IT1095369B (it) | 1985-08-10 |
| CH643661A5 (de) | 1984-06-15 |
| DE2718588B2 (de) | 1979-03-08 |
| IT7821633A0 (it) | 1978-03-24 |
| DK149476C (da) | 1986-12-01 |
| AU3378678A (en) | 1979-09-06 |
| FR2389135B1 (xx) | 1981-02-13 |
| AU511846B2 (en) | 1980-09-11 |
| DE2718588C3 (de) | 1979-11-08 |
| NL7803366A (nl) | 1978-10-30 |
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