SA519410901B1 - نيكلوتيدات معلمة بالشحنة وطرق استخدامها - Google Patents

نيكلوتيدات معلمة بالشحنة وطرق استخدامها

Info

Publication number
SA519410901B1
SA519410901B1 SA519410901A SA519410901A SA519410901B1 SA 519410901 B1 SA519410901 B1 SA 519410901B1 SA 519410901 A SA519410901 A SA 519410901A SA 519410901 A SA519410901 A SA 519410901A SA 519410901 B1 SA519410901 B1 SA 519410901B1
Authority
SA
Saudi Arabia
Prior art keywords
charge
methods
tagged nucleotides
polymerase
polynucleotide strand
Prior art date
Application number
SA519410901A
Other languages
English (en)
Inventor
ماريا كانديلاريا روجيرت باكيجالوبو،
كيتلين بوجليس
سيرجيو بيساجوفيش
ين ناه تيو
سيلفيا جرافنا
جيفيرى مانديل
اكسينجيوان يانج
Original Assignee
.الومينا، إنك
الليومينا سينغافورة بتى. ليمتد
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by .الومينا، إنك, الليومينا سينغافورة بتى. ليمتد filed Critical .الومينا، إنك
Publication of SA519410901B1 publication Critical patent/SA519410901B1/ar

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B70/00—Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/543—Immobilised enzyme(s)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122—Massive parallel sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/113—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being electroactive, e.g. redox labels

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

تم توفير طريقة تتضمن الكشف عن دمج نيكلوتيد معلم labelled nucleotide في جديلة بولي نيكلوتيد ناشئ nascent polynucleotide strand مكملة لجديلة بولي نيكلوتيد قالب template polynucleotide strand بواسطة بوليميراز polymerase، حيث يتم تجميع البوليميراز على قناة موصلة لدعامة صلبة solid support conductive channel بواسطة نطاق ربط والنيكلوتيدات المعلمة labelled nucleotides عبارة عن مركب من الصيغة I: شكل1.
SA519410901A 2018-02-16 2019-12-25 نيكلوتيدات معلمة بالشحنة وطرق استخدامها SA519410901B1 (ar)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201862710461P 2018-02-16 2018-02-16
US201862710327P 2018-02-16 2018-02-16
US201862710333P 2018-02-16 2018-02-16
US201862710362P 2018-02-16 2018-02-16
PCT/US2019/018565 WO2019161381A1 (en) 2018-02-16 2019-02-19 Charge-tagged nucleotides and methods of use thereof

Publications (1)

Publication Number Publication Date
SA519410901B1 true SA519410901B1 (ar) 2022-07-17

Family

ID=67619079

Family Applications (1)

Application Number Title Priority Date Filing Date
SA519410901A SA519410901B1 (ar) 2018-02-16 2019-12-25 نيكلوتيدات معلمة بالشحنة وطرق استخدامها

Country Status (16)

Country Link
US (2) US10851131B2 (ar)
EP (1) EP3752631A4 (ar)
JP (2) JP6938690B2 (ar)
KR (2) KR102330071B1 (ar)
CN (1) CN111164222A (ar)
AU (2) AU2019221691B2 (ar)
BR (1) BR112019027628A2 (ar)
CA (1) CA3067433C (ar)
IL (1) IL271303A (ar)
MX (1) MX2019015011A (ar)
PH (1) PH12019502905A1 (ar)
RU (1) RU2751359C2 (ar)
SA (1) SA519410901B1 (ar)
SG (1) SG11201912273WA (ar)
WO (1) WO2019161381A1 (ar)
ZA (1) ZA201908276B (ar)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX2019015011A (es) 2018-02-16 2021-01-08 Illumina Inc Nucleótidos etiquetados con carga y métodos de uso de los mismos.
KR102429576B1 (ko) * 2020-04-29 2022-08-03 경희대학교 산학협력단 전도성 태그를 이용한 dna 염기서열을 식별하는 방법 및 장치

Family Cites Families (24)

* Cited by examiner, † Cited by third party
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EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
US20090186343A1 (en) * 2003-01-28 2009-07-23 Visigen Biotechnologies, Inc. Methods for preparing modified biomolecules, modified biomolecules and methods for using same
US8637650B2 (en) * 2003-11-05 2014-01-28 Genovoxx Gmbh Macromolecular nucleotide compounds and methods for using the same
CN101460953B (zh) 2006-03-31 2012-05-30 索雷克萨公司 用于合成分析的序列的系统和装置
AU2009251883B2 (en) 2008-03-31 2014-09-11 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
EP2346889B1 (en) 2008-09-03 2015-11-11 Quantumdx Group Limited Design, synthesis and use of synthetic nucleotides comprising ionic reporter groups
US8632975B2 (en) 2009-06-05 2014-01-21 Life Technologies Corporation Nucleotide transient binding for sequencing methods
WO2012009206A2 (en) 2010-07-12 2012-01-19 Pacific Biosciences Of California, Inc. Sequencing reactions with alkali metal cations for pulse width control
US9164053B2 (en) 2011-09-26 2015-10-20 The Regents Of The University Of California Electronic device for monitoring single molecule dynamics
JP5970049B2 (ja) * 2013-11-28 2016-08-17 日本特殊陶業株式会社 スパークプラグおよびその製造方法
CN106715453B (zh) * 2014-03-24 2021-04-30 哥伦比亚大学董事会 用于生产带标签的核苷酸的化学方法
DK3169805T5 (en) 2014-07-15 2018-12-03 Illumina Inc Biochemically activated electronic device
SG11201708854PA (en) * 2015-05-12 2017-11-29 Illumina Inc Field-effect apparatus and methods for sequencing nucelic acids
EP3332033B1 (en) * 2015-08-06 2021-04-21 Pacific Biosciences of California, Inc. Single-molecule nanofet sequencing systems and methods
EP3347366A1 (en) * 2015-09-10 2018-07-18 H. Hoffnabb-La Roche Ag Polypeptide tagged nucleotides and use thereof in nucleic acid sequencing by nanopore detection
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US11225687B2 (en) * 2016-04-04 2022-01-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by synthesis with nucleotide analogues and Raman detection
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MX2019015011A (es) 2018-02-16 2021-01-08 Illumina Inc Nucleótidos etiquetados con carga y métodos de uso de los mismos.

Also Published As

Publication number Publication date
US10851131B2 (en) 2020-12-01
US20210179657A1 (en) 2021-06-17
US11578094B2 (en) 2023-02-14
SG11201912273WA (en) 2020-01-30
AU2022202417A1 (en) 2022-05-05
WO2019161381A9 (en) 2019-11-28
CA3067433A1 (en) 2019-08-22
PH12019502905A1 (en) 2020-09-14
CA3067433C (en) 2022-11-22
KR102330071B1 (ko) 2021-11-23
AU2019221691A1 (en) 2020-01-16
MX2019015011A (es) 2021-01-08
KR20210138136A (ko) 2021-11-18
RU2019141361A (ru) 2021-06-15
JP2020534790A (ja) 2020-12-03
EP3752631A1 (en) 2020-12-23
BR112019027628A2 (pt) 2020-08-25
AU2019221691B2 (en) 2022-04-28
IL271303A (en) 2020-01-30
KR102430323B1 (ko) 2022-08-08
US20200123193A1 (en) 2020-04-23
KR20200028344A (ko) 2020-03-16
JP2022000029A (ja) 2022-01-04
ZA201908276B (en) 2022-07-27
EP3752631A4 (en) 2021-11-10
CN111164222A (zh) 2020-05-15
RU2019141361A3 (ar) 2021-06-15
JP6938690B2 (ja) 2021-09-22
WO2019161381A1 (en) 2019-08-22
RU2751359C2 (ru) 2021-07-13

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