RU2758468C2 - Композиция, содержащая биосовместимый матрикс, способ получения композиции - Google Patents
Композиция, содержащая биосовместимый матрикс, способ получения композиции Download PDFInfo
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| RU2793889C1 (ru) * | 2022-04-22 | 2023-04-07 | Федеральное государственное бюджетное образовательное учреждение высшего образования ФГБОУ ВО "Пензенский государственный университет" | Способ активизации репаративных процессов в ранах у пациентов с синдромом диабетической стопы |
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| US11602579B2 (en) | 2017-09-20 | 2023-03-14 | Novadip Biosciences | Biomaterial comprising adipose-derived stem cells and method for producing the same |
| CN118001466A (zh) * | 2017-09-20 | 2024-05-10 | 诺瓦迪普生物科学股份公司 | 包含脂肪干细胞的生物材料及其制备方法 |
| CN108359637B (zh) * | 2018-02-14 | 2021-09-28 | 浙江生创精准医疗科技有限公司 | 一种宫血干细胞快速扩增的方法 |
| KR20210035776A (ko) | 2018-03-06 | 2021-04-01 | 에피본, 인크. | 주사가능한 기성품 연골, 힘줄 및 인대 복구 조성물 및 사용 방법 |
| JP7353652B2 (ja) * | 2018-04-25 | 2023-10-02 | 北海道公立大学法人 札幌医科大学 | 生体移植用細胞シート及びその製造方法 |
| SG11202101902QA (en) * | 2018-09-20 | 2021-04-29 | Novadip Biosciences | Biomaterial comprising adipose-derived stem cells and gelatin and method for producing the same |
| CN109224129B (zh) * | 2018-09-30 | 2021-09-24 | 四川大学华西医院 | 一种皮肤缺损修复材料 |
| MY198403A (en) * | 2019-06-12 | 2023-08-28 | Cryocord Sdn Bhd | Production of Therapeutics Potential Mesenchymal Stem Cells |
| CN114007655A (zh) * | 2019-06-14 | 2022-02-01 | 旗舰创业创新第六有限责任公司 | 用于细胞疗法的环状rna |
| WO2020262916A1 (ko) * | 2019-06-24 | 2020-12-30 | (주)메디노 | 말초 동맥 질환의 치료용 조성물 |
| CN112760293B (zh) * | 2021-01-08 | 2023-04-18 | 广东工业大学 | 一种无异源血清3d培养msc干细胞制备高活性外泌体的方法 |
| JP2024546382A (ja) * | 2021-12-24 | 2024-12-20 | イファ ユニバーシティ－インダストリー コラボレーション ファウンデーション | 皮膚由来幹細胞の培養方法及びその用途 |
| JP2023158764A (ja) * | 2022-04-19 | 2023-10-31 | 地方独立行政法人東京都立産業技術研究センター | 皮膚組織創傷癒合模倣材 |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090180965A1 (en) * | 2008-01-04 | 2009-07-16 | Boston Scientific Scimed, Inc. | Methods for repair and regeneration of bone marrow |
| RU2419441C2 (ru) * | 2003-08-07 | 2011-05-27 | Хилор Лтд. | Применение адипоцитов, преадипоцитов и стволовых клеток для индукции или ускорения заживления раны и фармацевтическая композиция, содержащая их |
| EP2374485A1 (en) * | 2010-04-09 | 2011-10-12 | Luisa Benassi | New dermal substitute and therapeutical application thereof |
| WO2014113704A2 (en) * | 2013-01-18 | 2014-07-24 | Escape Therapeutics, Inc. | Enhanced differentiation of mesenchymal stem cells |
| WO2014150784A1 (en) * | 2013-03-15 | 2014-09-25 | Allosource | Cell repopulated collagen matrix for soft tissue repair and regeneration |
Family Cites Families (10)
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| US8394371B2 (en) * | 2002-02-11 | 2013-03-12 | Neocutis Sa | Compositions for the treatment of skin conditions, disorders or diseases and methods of making and using the same |
| JP2004016109A (ja) * | 2002-06-17 | 2004-01-22 | Nagoya Industrial Science Research Inst | 軟骨細胞様細胞、及びその作製方法 |
| US20040136968A1 (en) * | 2002-09-27 | 2004-07-15 | Verigen Ag | Autologous cells on a support matrix for tissue repair |
| US20040101959A1 (en) * | 2002-11-21 | 2004-05-27 | Olga Marko | Treatment of tissue with undifferentiated mesenchymal cells |
| CN101472622B (zh) * | 2006-04-19 | 2014-11-26 | 国立大学法人名古屋大学 | 用于牙周软组织再生的组合物及其制备方法 |
| US9382514B2 (en) | 2008-06-20 | 2016-07-05 | Escape Therapeutics, Inc. | Compositions comprising mesenchymal stem cell-derived fibroblasts |
| JP2012065742A (ja) * | 2010-09-22 | 2012-04-05 | Atree Co Ltd | コラーゲン／アパタイト配向性材料、及びコラーゲン／アパタイト配向性材料の製造方法 |
| US20120087983A1 (en) * | 2010-09-24 | 2012-04-12 | Jointechlabs, Inc. | Orthopedic application of encapsulated stem cells |
| KR101138091B1 (ko) * | 2011-08-31 | 2012-04-24 | 세원셀론텍(주) | 중간엽 줄기세포 기본 배양 배지 조성방법, 중간엽 줄기세포 기본 배양 배지 및 이를 이용하여 배양분화된 세포치료제 |
| JP6081276B2 (ja) * | 2013-04-10 | 2017-02-15 | クアーズテック株式会社 | 細胞培養担体 |
-
2015
- 2015-07-31 EP EP15179418.7A patent/EP3090764A1/en not_active Withdrawn
-
2016
- 2016-05-09 AU AU2016259737A patent/AU2016259737A1/en not_active Abandoned
- 2016-05-09 CA CA2985272A patent/CA2985272C/en active Active
- 2016-05-09 US US15/571,460 patent/US20180126039A1/en not_active Abandoned
- 2016-05-09 ES ES16725382T patent/ES2892974T3/es active Active
- 2016-05-09 BR BR112017024013-0A patent/BR112017024013A2/pt not_active Application Discontinuation
- 2016-05-09 JP JP2017557916A patent/JP6966332B2/ja not_active Expired - Fee Related
- 2016-05-09 CN CN201680037547.6A patent/CN107810014B/zh active Active
- 2016-05-09 MX MX2017014245A patent/MX390493B/es unknown
- 2016-05-09 EP EP16725382.2A patent/EP3294356B1/en active Active
- 2016-05-09 RU RU2017139096A patent/RU2758468C2/ru not_active Application Discontinuation
- 2016-05-09 WO PCT/EP2016/060352 patent/WO2016180789A1/en not_active Ceased
- 2016-05-09 KR KR1020177034751A patent/KR20180008526A/ko not_active Ceased
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2017
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- 2017-11-06 IL IL255480A patent/IL255480B/en unknown
-
2020
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-
2021
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-
2022
- 2022-09-30 AU AU2022241581A patent/AU2022241581A1/en not_active Abandoned
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2419441C2 (ru) * | 2003-08-07 | 2011-05-27 | Хилор Лтд. | Применение адипоцитов, преадипоцитов и стволовых клеток для индукции или ускорения заживления раны и фармацевтическая композиция, содержащая их |
| US20090180965A1 (en) * | 2008-01-04 | 2009-07-16 | Boston Scientific Scimed, Inc. | Methods for repair and regeneration of bone marrow |
| EP2374485A1 (en) * | 2010-04-09 | 2011-10-12 | Luisa Benassi | New dermal substitute and therapeutical application thereof |
| WO2014113704A2 (en) * | 2013-01-18 | 2014-07-24 | Escape Therapeutics, Inc. | Enhanced differentiation of mesenchymal stem cells |
| WO2014150784A1 (en) * | 2013-03-15 | 2014-09-25 | Allosource | Cell repopulated collagen matrix for soft tissue repair and regeneration |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2793889C1 (ru) * | 2022-04-22 | 2023-04-07 | Федеральное государственное бюджетное образовательное учреждение высшего образования ФГБОУ ВО "Пензенский государственный университет" | Способ активизации репаративных процессов в ранах у пациентов с синдромом диабетической стопы |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107810014A (zh) | 2018-03-16 |
| KR20180008526A (ko) | 2018-01-24 |
| MX2017014245A (es) | 2018-06-28 |
| US20180126039A1 (en) | 2018-05-10 |
| WO2016180789A1 (en) | 2016-11-17 |
| CA2985272C (en) | 2023-10-17 |
| JP2018515211A (ja) | 2018-06-14 |
| CA2985272A1 (en) | 2016-11-17 |
| AU2022241581A1 (en) | 2022-10-27 |
| AU2020207792A1 (en) | 2020-08-06 |
| JP2021184828A (ja) | 2021-12-09 |
| ES2892974T3 (es) | 2022-02-07 |
| BR112017024013A2 (pt) | 2018-07-17 |
| RU2017139096A (ru) | 2019-06-10 |
| AU2016259737A1 (en) | 2017-11-30 |
| ZA201707478B (en) | 2020-05-27 |
| EP3294356A1 (en) | 2018-03-21 |
| MX390493B (es) | 2025-03-20 |
| CN107810014B (zh) | 2021-05-28 |
| IL255480A (en) | 2018-01-31 |
| JP6966332B2 (ja) | 2021-11-17 |
| EP3090764A1 (en) | 2016-11-09 |
| RU2017139096A3 (https=) | 2019-07-29 |
| IL255480B (en) | 2021-07-29 |
| EP3294356B1 (en) | 2021-07-07 |
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Legal Events
| Date | Code | Title | Description |
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| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20191115 |
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| FZ9A | Application not withdrawn (correction of the notice of withdrawal) |
Effective date: 20191224 |