RU2708992C2 - Аллель-специфическая амплификация с использованием композиции перекрывающихся олигонуклеотидов в качестве не являющегося аллель-специфическим праймера и аллель-специфического блокатора - Google Patents

Аллель-специфическая амплификация с использованием композиции перекрывающихся олигонуклеотидов в качестве не являющегося аллель-специфическим праймера и аллель-специфического блокатора Download PDF

Info

Publication number
RU2708992C2
RU2708992C2 RU2016149307A RU2016149307A RU2708992C2 RU 2708992 C2 RU2708992 C2 RU 2708992C2 RU 2016149307 A RU2016149307 A RU 2016149307A RU 2016149307 A RU2016149307 A RU 2016149307A RU 2708992 C2 RU2708992 C2 RU 2708992C2
Authority
RU
Russia
Prior art keywords
sequence
subsequence
oligonucleotide
target
blocker
Prior art date
Application number
RU2016149307A
Other languages
English (en)
Russian (ru)
Other versions
RU2016149307A (ru
RU2016149307A3 (show.php
Inventor
Дэвид Юй ЧЖАН
Жоцзя У
Цзюэсяо ВАН
Original Assignee
Уильям Марш Райс Юниверсити
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Уильям Марш Райс Юниверсити filed Critical Уильям Марш Райс Юниверсити
Publication of RU2016149307A publication Critical patent/RU2016149307A/ru
Publication of RU2016149307A3 publication Critical patent/RU2016149307A3/ru
Application granted granted Critical
Publication of RU2708992C2 publication Critical patent/RU2708992C2/ru

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/686Polymerase chain reaction [PCR]
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00Methods of screening libraries
    • C40B30/04Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00Reactions demanding special reaction conditions
    • C12Q2527/107Temperature of melting, i.e. Tm
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/163Reactions characterised by the reaction format or use of a specific feature the purpose or use of blocking probe

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
RU2016149307A 2014-05-19 2015-05-19 Аллель-специфическая амплификация с использованием композиции перекрывающихся олигонуклеотидов в качестве не являющегося аллель-специфическим праймера и аллель-специфического блокатора RU2708992C2 (ru)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462000114P 2014-05-19 2014-05-19
US62/000,114 2014-05-19
PCT/US2015/031476 WO2015179339A1 (en) 2014-05-19 2015-05-19 Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides

Publications (3)

Publication Number Publication Date
RU2016149307A RU2016149307A (ru) 2018-06-20
RU2016149307A3 RU2016149307A3 (show.php) 2018-12-10
RU2708992C2 true RU2708992C2 (ru) 2019-12-12

Family

ID=54554607

Family Applications (1)

Application Number Title Priority Date Filing Date
RU2016149307A RU2708992C2 (ru) 2014-05-19 2015-05-19 Аллель-специфическая амплификация с использованием композиции перекрывающихся олигонуклеотидов в качестве не являющегося аллель-специфическим праймера и аллель-специфического блокатора

Country Status (13)

Country Link
US (2) US20170067090A1 (show.php)
EP (3) EP4512908A3 (show.php)
JP (4) JP2017515484A (show.php)
KR (1) KR102343605B1 (show.php)
CN (2) CN106661627B (show.php)
AU (2) AU2015264357B2 (show.php)
BR (1) BR112016027063A2 (show.php)
CA (1) CA2985631A1 (show.php)
DK (1) DK3146080T3 (show.php)
ES (1) ES2861353T3 (show.php)
PH (1) PH12016502542B1 (show.php)
RU (1) RU2708992C2 (show.php)
WO (1) WO2015179339A1 (show.php)

Families Citing this family (55)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP3321378B1 (en) 2012-02-27 2021-11-10 Becton, Dickinson and Company Compositions for molecular counting
ES2711168T3 (es) 2013-08-28 2019-04-30 Becton Dickinson Co Análisis masivo en paralelo de células individuales
WO2015077717A1 (en) 2013-11-25 2015-05-28 The Broad Institute Inc. Compositions and methods for diagnosing, evaluating and treating cancer by means of the dna methylation status
WO2015085147A1 (en) 2013-12-05 2015-06-11 The Broad Institute Inc. Polymorphic gene typing and somatic change detection using sequencing data
JP7060324B2 (ja) 2013-12-20 2022-04-26 ザ・ブロード・インスティテュート・インコーポレイテッド ネオ抗原ワクチンによる併用療法
ES3049406T3 (en) 2014-12-19 2025-12-16 Broad Inst Inc Methods for profiling the t-cell-receptor repertoire
EP3234193B1 (en) 2014-12-19 2020-07-15 Massachusetts Institute of Technology Molecular biomarkers for cancer immunotherapy
ES2836802T3 (es) 2015-02-27 2021-06-28 Becton Dickinson Co Códigos de barras moleculares espacialmente direccionables
ES2934982T3 (es) 2015-03-30 2023-02-28 Becton Dickinson Co Métodos para la codificación con códigos de barras combinatorios
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
EP3347465B1 (en) 2015-09-11 2019-06-26 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
CN108603193B (zh) 2015-12-30 2022-07-22 生物辐射实验室股份有限公司 分裂循环和tape扩增
WO2017193025A1 (en) 2016-05-06 2017-11-09 William Marsh Rice University Stoichiometric nucleic acid purification using randomer capture probe libraries
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
EP3516400B1 (en) 2016-09-26 2023-08-16 Becton, Dickinson and Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
EP3574116A1 (en) * 2017-01-24 2019-12-04 The Broad Institute, Inc. Compositions and methods for detecting a mutant variant of a polynucleotide
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
WO2019079656A1 (en) 2017-10-18 2019-04-25 Day Zero Diagnostics, Inc. SELECTIVE ENRICHMENT OF DNA POPULATION IN MIXED DNA SAMPLE BY TARGETED SUPPRESSION OF DNA AMPLIFICATION
CN111683958A (zh) * 2018-02-20 2020-09-18 威廉马歇莱思大学 使用多重抑制探针置换扩增进行等位基因富集的系统和方法
CN108220444A (zh) * 2018-02-28 2018-06-29 无锡禾盛医疗器械有限公司 一种kras基因突变检测的引物探针组合及其应用
JP7358388B2 (ja) 2018-05-03 2023-10-10 ベクトン・ディキンソン・アンド・カンパニー 反対側の転写物末端における分子バーコーディング
ES2945191T3 (es) 2018-05-03 2023-06-29 Becton Dickinson Co Análisis de muestras multiómicas de alto rendimiento
US11639517B2 (en) 2018-10-01 2023-05-02 Becton, Dickinson And Company Determining 5′ transcript sequences
JP7618548B2 (ja) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
EP3911754A4 (en) * 2019-01-15 2022-10-12 Tangen Biosciences Inc. METHOD FOR REMOVAL OF NON-SPECIFIC AMPLIFICATION PRODUCTS IN NUCLEIC ACID AMPLIFICATION TECHNOLOGIES
EP4242322B1 (en) 2019-01-23 2024-08-21 Becton, Dickinson and Company Oligonucleotides associated with antibodies
WO2020167920A1 (en) 2019-02-14 2020-08-20 Cellular Research, Inc. Hybrid targeted and whole transcriptome amplification
WO2020259678A1 (zh) * 2019-06-26 2020-12-30 南京金斯瑞生物科技有限公司 一种含有阻断物的寡核苷酸
CN114051534B (zh) 2019-07-22 2025-02-21 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長v(d)j情報を得るためのランダムプライミングの使用
WO2021125854A1 (ko) * 2019-12-20 2021-06-24 고려대학교 산학협력단 극소량의 희귀 단일 염기 변이체 검출용 프라이머 및 이를 이용하여 극소량의 희귀 단일 염기 변이체를 특이적이고 민감하게 검출하는 방법
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
CN115335520A (zh) 2020-01-29 2022-11-11 贝克顿迪金森公司 用于通过测序对单细胞进行空间映射的条形码化的孔
EP4111168A1 (en) 2020-02-25 2023-01-04 Becton Dickinson and Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
KR20230006852A (ko) * 2020-05-01 2023-01-11 윌리엄 마쉬 라이스 유니버시티 무보정 및 다중 변이체 대립유전자 빈도 정량화를 위한 정량적 블로커 변위 증폭(qbda) 시퀀싱
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司(Us) 用于免疫组库谱分析的引物
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
KR20230028450A (ko) * 2020-06-26 2023-02-28 윌리엄 마쉬 라이스 유니버시티 앰플리콘 포괄적 풍부화
CN111647650B (zh) * 2020-07-06 2023-06-27 河南赛诺特生物技术有限公司 检测人B-raf基因V600E突变的引物、引物探针组合物及试剂盒
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CN116547390A (zh) * 2020-11-02 2023-08-04 阅尔基因美国公司 定量多重扩增子测序系统
CN116635533A (zh) 2020-11-20 2023-08-22 贝克顿迪金森公司 高表达的蛋白和低表达的蛋白的谱分析
CN112522369A (zh) * 2020-12-08 2021-03-19 合肥欧创基因生物科技有限公司 一种ARMS-TaqMan Blocker体系的Blocker双链设计方法
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析
WO2022146773A1 (en) 2020-12-29 2022-07-07 Nuprobe Usa, Inc. Methods and compositions for sequencing and fusion detection using ligation tail adapters (lta)
WO2022155397A1 (en) 2021-01-15 2022-07-21 Nuprobe Usa, Inc. Cycle multiplexing for highly multiplexed quantitative pcr
WO2022197688A1 (en) 2021-03-16 2022-09-22 Nuprobe Usa, Inc. High sensitivity detection of microsatellite loci by blocker displacement amplification with multiple blockers and its use in microsatellite instability detection
WO2023077121A1 (en) * 2021-11-01 2023-05-04 Nuprobe Usa, Inc. Rna quantitative amplicon sequencing for gene expression quantitation
CN120624641A (zh) * 2025-02-20 2025-09-12 宁波熙宁检测技术有限公司 一种用于检测ezh2基因突变的核酸组合物、试剂盒及检测方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090053720A1 (en) * 2007-08-08 2009-02-26 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' nuclease activity
RU2495046C2 (ru) * 2004-10-18 2013-10-10 Брандейс Юнивесити Реагент и способ предотвращения по меньшей мере одного ошибочного старта в амплификации полимеразной цепной реакции, наборы реагентов и олигонуклеотидов для осуществления амплификации посредством полимеразной цепной реакции

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
US20100009355A1 (en) * 2006-03-14 2010-01-14 Kolodney Michael S Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
CN101889096B (zh) * 2007-10-04 2015-10-21 联邦科学及工业研究组织 核酸扩增
CN105200097A (zh) * 2008-10-20 2015-12-30 霍夫曼-拉罗奇有限公司 改进的等位基因特异性扩增
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
JP5805064B2 (ja) * 2009-03-27 2015-11-04 ライフ テクノロジーズ コーポレーション 対立遺伝子変種を検出するための方法、組成物、およびキット
KR101312241B1 (ko) * 2010-04-27 2013-09-27 사회복지법인 삼성생명공익재단 증폭억제시발체를 이용하는 유전자 돌연변이 검출 방법
WO2011146403A2 (en) * 2010-05-16 2011-11-24 Reniguard Life Sciences, Inc. Identification of polymorphic hepatitis b viruses and kras oncogene mutations and clinical use
GB2506760B (en) * 2011-01-14 2015-07-22 Genefirst Ltd Allele specific primers for EGFR exon 21 specific mutations
EP3382039B1 (en) * 2011-05-04 2023-09-13 Biocept, Inc. Methods for detecting nucleic acid sequence variants
EP2798078B1 (en) * 2011-12-30 2018-02-14 Polowang Biotech Inc. Method and primer set for detecting mutation
CA2871704C (en) * 2012-04-25 2022-10-18 Tho Huu HO Method for competitive allele-specific cdna synthesis and differential amplification of the cdna products
CN103215361A (zh) * 2013-04-18 2013-07-24 深圳联合医学科技有限公司 等位基因变体检测方法、试剂盒及组合物
WO2014177540A1 (en) * 2013-04-29 2014-11-06 Qiagen Marseille A method for dna amplification with a blocking oligonucleotide

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2495046C2 (ru) * 2004-10-18 2013-10-10 Брандейс Юнивесити Реагент и способ предотвращения по меньшей мере одного ошибочного старта в амплификации полимеразной цепной реакции, наборы реагентов и олигонуклеотидов для осуществления амплификации посредством полимеразной цепной реакции
US20090053720A1 (en) * 2007-08-08 2009-02-26 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5'-3' nuclease activity

Also Published As

Publication number Publication date
US20220090168A1 (en) 2022-03-24
EP4512908A2 (en) 2025-02-26
JP2024167406A (ja) 2024-12-03
KR102343605B1 (ko) 2021-12-27
CA2985631A1 (en) 2015-11-26
RU2016149307A (ru) 2018-06-20
EP3146080A1 (en) 2017-03-29
CN114032234A (zh) 2022-02-11
JP2022166131A (ja) 2022-11-01
AU2015264357A1 (en) 2017-01-05
CN106661627B (zh) 2021-11-23
EP3901278B1 (en) 2024-10-23
PH12016502542A1 (en) 2017-04-10
AU2021266291A1 (en) 2021-12-09
BR112016027063A2 (pt) 2017-10-31
DK3146080T3 (en) 2021-04-12
EP4512908A3 (en) 2025-03-26
AU2021266291C1 (en) 2024-12-12
JP7126718B2 (ja) 2022-08-29
AU2015264357B2 (en) 2021-08-12
PH12016502542B1 (en) 2024-01-17
JP2021019607A (ja) 2021-02-18
JP2017515484A (ja) 2017-06-15
CN106661627A (zh) 2017-05-10
EP3901278A1 (en) 2021-10-27
RU2016149307A3 (show.php) 2018-12-10
US20170067090A1 (en) 2017-03-09
AU2021266291B2 (en) 2024-09-19
KR20170003695A (ko) 2017-01-09
ES2861353T3 (es) 2021-10-06
EP3146080B1 (en) 2021-01-06
EP3146080A4 (en) 2017-11-01
WO2015179339A1 (en) 2015-11-26

Similar Documents

Publication Publication Date Title
RU2708992C2 (ru) Аллель-специфическая амплификация с использованием композиции перекрывающихся олигонуклеотидов в качестве не являющегося аллель-специфическим праймера и аллель-специфического блокатора
EP2756101A2 (en) Probe: antiprobe compositions for high specificity dna or rna detection
CN106687589B (zh) Dna扩增技术
US20160010152A1 (en) Dual Probe:Antiprobe Compositions for DNA and RNA Detection
US7618773B2 (en) Headloop DNA amplification
EP3377652B1 (en) A method and a kit for nucleic acid detection
US11180798B2 (en) Modified primers for nucleic acid amplification and detection
US10822647B2 (en) Methods for using long ssDNA polynucleotides as primers (superprimers) in PCR assays
JP2018533943A (ja) 一塩基多型検出用キット及び方法
HK40065933A (en) Allele-specific amplification using overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
HK1236578A1 (en) Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
HK1236578B (zh) 使用重叠非等位基因特异性引物和等位基因特异性阻断剂寡核苷酸的组合物进行等位基因特异性扩增
EP3377651B1 (en) A method and a kit for nucleic acid detection
JP2025506964A (ja) 超特異的な分子の検出、形質導入、及び増幅のためのカスケード核酸プロトコル
KR20210098571A (ko) 반코마이신 내성 a균 검출용 유전자 마커에 특이적으로 결합가능한 프로브, 이를 포함하는 반코마이신 내성 a균 검출용 조성물, 검출용 키트 및 이를 이용한 반코마이신 내성 a균 검출방법