RU2646498C2 - Способы детектирования 5Т4-положительных циркулирующих опухолевых клеток и способы диагностики 5Т4-положительного рака у млекопитающего - Google Patents

Способы детектирования 5Т4-положительных циркулирующих опухолевых клеток и способы диагностики 5Т4-положительного рака у млекопитающего Download PDF

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RU2646498C2
RU2646498C2 RU2014127474A RU2014127474A RU2646498C2 RU 2646498 C2 RU2646498 C2 RU 2646498C2 RU 2014127474 A RU2014127474 A RU 2014127474A RU 2014127474 A RU2014127474 A RU 2014127474A RU 2646498 C2 RU2646498 C2 RU 2646498C2
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marker
blood sample
cells
absence
circulating tumor
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Ханс-Питер ГЕРБЕР
Дена Марринуччи
Стивен ПАЙРИ-ШЕПЕРД
Эрик ТАКЕР
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Пфайзер Инк.
Эпик Сайенсиз, Инк.
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    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5011—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing antineoplastic activity
    • G—PHYSICS
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
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    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/575—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/5758—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumours, cancers or neoplasias, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides or metabolites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
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RU2014127474A 2012-01-24 2013-01-22 Способы детектирования 5Т4-положительных циркулирующих опухолевых клеток и способы диагностики 5Т4-положительного рака у млекопитающего RU2646498C2 (ru)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261590066P 2012-01-24 2012-01-24
US61/590,066 2012-01-24
US201361753665P 2013-01-17 2013-01-17
US61/753,665 2013-01-17
PCT/IB2013/050547 WO2013111054A1 (en) 2012-01-24 2013-01-22 Methods for detecting 5t4-positive circulating tumor cells and methods of diagnosis of 5t4-positive cancer in a mammalian subject

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RU2014127474A RU2014127474A (ru) 2016-03-20
RU2646498C2 true RU2646498C2 (ru) 2018-03-05

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US (1) US10254286B2 (2)
EP (1) EP2807485B1 (2)
JP (1) JP6254951B2 (2)
KR (1) KR101716555B1 (2)
CN (1) CN104094116B (2)
AU (2) AU2013213301A1 (2)
BR (1) BR112014018192A8 (2)
CA (1) CA2861437C (2)
ES (1) ES2724232T3 (2)
IL (1) IL233543B (2)
IN (1) IN2014DN07019A (2)
MX (1) MX361058B (2)
RU (1) RU2646498C2 (2)
WO (1) WO2013111054A1 (2)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2845800C1 (ru) * 2024-12-24 2025-08-25 Федеральное государственное бюджетное образовательное учреждение высшего образования "Красноярский государственный медицинский университет имени профессора В.Ф.Войно-Ясенецкого" Министерства здравоохранения Российской Федерации Способ дифференциальной диагностики рака мочевого пузыря, рака почки и рака легкого

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2803804B2 (ja) 1992-08-21 1998-09-24 株式会社長澤製作所 ボタン錠
EP3045918B1 (en) 2009-10-21 2017-12-06 The Scripps Research Institute Method of using non-rare cells to detect rare cells
US8956859B1 (en) 2010-08-13 2015-02-17 Aviex Technologies Llc Compositions and methods for determining successful immunization by one or more vaccines
EA201691352A1 (ru) * 2013-12-30 2016-11-30 Дзе Скриппс Рисерч Инститьют Диагностика рака легкого с помощью циркулирующих опухолевых клеток
EA201691496A1 (ru) 2014-01-27 2016-12-30 Эпик Сайенсиз, Инк. Диагностика биомаркеров рака предстательной железы с помощью циркулирующих опухолевых клеток
EA201691682A1 (ru) * 2014-02-21 2017-02-28 Эпик Сайенсиз, Инк. Способы анализирования редких циркулирующих в крови клеток
WO2015155345A1 (en) * 2014-04-11 2015-10-15 Medimmune Limited Antibodies and antibody-drug conjugates
EA201692223A1 (ru) * 2014-05-09 2017-04-28 Дзе Скриппс Рисерч Инститьют Композиции и способы жидкостной биопсии меланомы
CA2959072C (en) * 2014-08-25 2025-12-09 Creatv Microtech, Inc. Use of circulating cell biomarkers in the blood for detection and diagnosis of diseases and methods of isolating them
CN113552350B (zh) * 2015-04-21 2024-10-11 基因泰克公司 用于前列腺癌分析的组合物和方法
KR102771841B1 (ko) * 2015-11-06 2025-02-24 벤타나 메디컬 시스템즈, 인코포레이티드 대표 진단법
KR20170099738A (ko) 2016-02-23 2017-09-01 노을 주식회사 접촉식 염색 패치 및 그 제조 방법
KR102406596B1 (ko) * 2016-02-23 2022-06-10 노을 주식회사 패치를 이용하는 ctc 진단 방법 및 장치
KR102478639B1 (ko) 2016-02-23 2022-12-19 노을 주식회사 표지 물질을 저장하는 패치, 이를 이용하는 조직 진단 방법 및 장치
US10371610B2 (en) 2016-02-23 2019-08-06 Noul Co., Ltd. Contact-type patch, staining method using the same, and manufacturing method thereof
KR102045069B1 (ko) * 2016-02-23 2019-11-15 노을 주식회사 패치를 이용하는 ctc 진단 방법 및 장치
SG11201909218RA (en) 2017-04-03 2019-11-28 Hoffmann La Roche Antibodies binding to steap-1
EP3646010A4 (en) * 2017-06-27 2020-11-11 Qcdx, Llc QUANTITATIVE LIQUID BIOPSY DIAGNOSTIC SYSTEM AND METHOD
CN107918013A (zh) * 2017-09-25 2018-04-17 浙江天科高新技术发展有限公司 化学发光酶免测定循环肿瘤细胞中K‑Ras蛋白的方法及试剂盒
JP7678424B2 (ja) 2018-05-10 2025-05-16 学校法人順天堂 画像解析方法、装置、コンピュータプログラム、及び深層学習アルゴリズムの生成方法
WO2020086882A1 (en) * 2018-10-24 2020-04-30 Northwestern University Tumor cell aggregation inhibitors' for treating cancer
JP7381003B2 (ja) * 2019-04-26 2023-11-15 学校法人順天堂 疾患解析を支援する方法、装置、及びコンピュータプログラム、並びにコンピュータアルゴリズムを訓練する方法、装置、及びプログラム
EP4445141A1 (en) 2021-12-07 2024-10-16 Epic Sciences, Inc. Enhanced characterization of breast cancer

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007089911A2 (en) * 2006-01-30 2007-08-09 The Scripps Research Institute Methods for detection of circulating tumor cells and methods of diagnosis of cancer in a mammalian subject
WO2010070276A1 (en) * 2008-12-15 2010-06-24 Oxford Biomedica (Uk) Limited Method

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4391904A (en) 1979-12-26 1983-07-05 Syva Company Test strip kits in immunoassays and compositions therein
US5869053A (en) 1988-03-04 1999-02-09 Cancer Research Campaign Technology, Ltd. 5T4 antigen from human trophoblasts
WO1989007947A1 (en) 1988-03-04 1989-09-08 Cancer Research Campaign Technology Limited Improvements relating to antigens
EP1717248A1 (en) 1997-06-04 2006-11-02 Oxford Biomedica (UK) Limited Tumor targeted vector
JP2003515323A (ja) 1999-11-18 2003-05-07 オックスフォード バイオメディカ（ユーケイ）リミテッド 抗 体
GB0126378D0 (en) 2001-11-02 2002-01-02 Oxford Biomedica Ltd Antigen
US7305112B2 (en) 2002-10-15 2007-12-04 The Scripps Research Institute Method of converting rare cell scanner image coordinates to microscope coordinates using reticle marks on a sample media
PE20100251A1 (es) * 2004-09-10 2010-04-10 Wyeth Corp Anticuerpos anti-5t4 humanizados y conjugados anticuerpo anti-5t4/calicheamicina
MY148763A (en) 2006-03-10 2013-05-31 Wyeth Corp Anti-5t4 antibodies and uses thereof
US20100233694A1 (en) 2007-04-16 2010-09-16 On-O-ity, Inc Devices and methods for diagnosing, prognosing, or theranosing a condition by enriching rare cells
AU2010229924B2 (en) 2009-03-24 2016-07-21 Plus Therapeutics Inc. Devices and methods of cell capture and analysis
ES2405605T3 (es) 2009-03-27 2013-05-31 Wyeth Llc Células iniciadoras de tumores y procedimientos de uso de las mismas
JP2013504064A (ja) 2009-09-03 2013-02-04 ザ スクリプス リサーチ インスティチュート 循環腫瘍細胞を分類する方法
EP3045918B1 (en) 2009-10-21 2017-12-06 The Scripps Research Institute Method of using non-rare cells to detect rare cells
WO2011127219A1 (en) * 2010-04-06 2011-10-13 Caris Life Sciences Luxembourg Holdings Circulating biomarkers for disease
EP2591363B1 (en) 2010-07-07 2017-09-20 The Regents Of The University Of Michigan Diagnosis and treatment of breast cancer
DK2694111T3 (en) 2011-04-01 2016-10-10 Wyeth Llc Antibody pharmaceutical conjugates

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007089911A2 (en) * 2006-01-30 2007-08-09 The Scripps Research Institute Methods for detection of circulating tumor cells and methods of diagnosis of cancer in a mammalian subject
WO2010070276A1 (en) * 2008-12-15 2010-06-24 Oxford Biomedica (Uk) Limited Method

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
MYERS K.A. et al. Metastasis-associated 5T4 antigen disrupts cell-cell contacts and induces cellular motility in epithelial cells// International Journal of Cancer Volume 68, Issue 1, pages 84-92, 27 September 1996 [он лайн] [найдено 10.11.2015] найдено в Интернет: 3.0.CO;2-6/epdf"http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1097-0215(19960927)68:1<84::AID-IJC15>3.0.CO;2-6/epdf. *
ZHAO Y et al. 5T4 oncotrophoblast glycoprotein: janus molecule in life and a novel potential target against tumors// Cell Mol Immunol. 2007 Apr;4(2):99-104 [он лайн] [найдено 10.11.2015] найденов Интернет: http://www.cm.ustc.edu.cn/4/2/99.pdf . *
ZHAO Y et al. 5T4 oncotrophoblast glycoprotein: janus molecule in life and a novel potential target against tumors// Cell Mol Immunol. 2007 Apr;4(2):99-104 [он лайн] [найдено 10.11.2015] найденов Интернет: http://www.cm.ustc.edu.cn/4/2/99.pdf . MYERS K.A. et al. Metastasis-associated 5T4 antigen disrupts cell-cell contacts and induces cellular motility in epithelial cells// International Journal of Cancer Volume 68, Issue 1, pages 84-92, 27 September 1996 [он лайн] [найдено 10.11.2015] найдено в Интернет: 3.0.CO;2-6/epdf"http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1097-0215(19960927)68:1<84::AID-IJC15>3.0.CO;2-6/epdf. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2845800C1 (ru) * 2024-12-24 2025-08-25 Федеральное государственное бюджетное образовательное учреждение высшего образования "Красноярский государственный медицинский университет имени профессора В.Ф.Войно-Ясенецкого" Министерства здравоохранения Российской Федерации Способ дифференциальной диагностики рака мочевого пузыря, рака почки и рака легкого

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IL233543B (en) 2018-02-28
WO2013111054A1 (en) 2013-08-01
MX2014008928A (es) 2014-10-24
US20150017661A1 (en) 2015-01-15
CN104094116B (zh) 2017-05-31
KR101716555B1 (ko) 2017-03-14
CA2861437A1 (en) 2013-08-01
IL233543A0 (en) 2014-08-31
CN104094116A (zh) 2014-10-08
RU2014127474A (ru) 2016-03-20
IN2014DN07019A (2) 2015-04-10
ES2724232T3 (es) 2019-09-09
MX361058B (es) 2018-11-23
AU2013213301A1 (en) 2014-07-17
BR112014018192A8 (pt) 2021-03-02
JP2015507190A (ja) 2015-03-05
CA2861437C (en) 2018-08-21
JP6254951B2 (ja) 2017-12-27
KR20140100580A (ko) 2014-08-14
HK1202923A1 (en) 2015-10-09
AU2018220154A1 (en) 2018-09-13
EP2807485A1 (en) 2014-12-03
US10254286B2 (en) 2019-04-09
EP2807485B1 (en) 2019-03-13

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