RU2517063C1 - Water-soluble bactericidal composition - Google Patents

Abstract

FIELD: medicine.

SUBSTANCE: composition contains the bactericidal substance Poviargol in the amount of 2.1-7.0 wt % and zosterin in the amount of 1.1-7.0 wt %.

EFFECT: synergetic action of the ingredients of the composition and promotion of the anti-inflammatory, radio protective, reparative action.

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Description

The invention relates to medicine, veterinary medicine, namely to the field of combustology, dermatology, surgery, medical cosmetology and is used both for prevention and for the treatment of humans and animals.

It is known the use of compositions as wound healing, dermatological agents containing immunomodulators, tissue metabolism stimulators, etc.

Known composition of the ingredients for the syrup-balsam herbamarin, providing a comprehensive therapeutic and prophylactic effect. However, this composition, due to its many components, in addition to zosterol, contributes to allergic reactions, especially in children [RU 2092077, A23L 1/09, A23L 1/30, A61K 35/78, 1997].

A known method of treating mastitis in cows for veterinary medicine, which includes the introduction of a biogenic stimulant in the form of a tissue emulsion. As a biogenic stimulator, a tissue emulsion is used, obtained from the udder, caruncle of the pregnant woman or after childbirth of the uterus, thymus, thoracic and abdominal aorta of cattle up to 4-6 years of age. In this case, the udder, caruncles of the uterus, thymus and the thoracic and abdominal aorta are taken in an amount of 3; 2.5; 2.5 and 2 parts by weight respectively. Then, f-2 ASD, potassium iodide, caffeine, sodium benzoate and formalin are added to the tissue emulsion. The method is characterized by the difficulty of obtaining the starting preparations from animal tissues, their technological processing, the possibility of manifesting strong side effects [RU 2340332, A61K 31/00, A61K 35/26, A61K 35/44, A61K 35/48, 2008].

Aqueous solutions of zosterol are characterized by low storage stability (they bloom, their surfaces are covered with mold), which excludes their use for the treatment of wounds and burns and requires sterilization immediately before use.

In addition, various wound healing silver-containing bactericidal preparations are known, for example protargol, poviargol.

The closest tool to the proposed is a water-soluble bactericidal composition and a method for its preparation, containing the following ingredients, parts by weight: poly-N-vinylpyrrolidone-2 with MM 8 · 10 ³ -1.63 · 10 ⁶ 1; fine metallic silver 0.01-2.33. Also proposed is a method for producing such compositions, which is realized by the interaction of silver nitrate with an aqueous-alcoholic solution of poly-N-vinylpyrididone-2 at the following concentrations of ingredients, wt.%: Poly-N-vinylpyrrolidone-2 0,065-11,00; silver nitrate 0.25-29.73; ethyl alcohol 5.0-38.6; water - the rest is up to 100. The interaction is carried out in the dark at 65-75 ° C in an inert gas atmosphere [RU 2088234, A61K 31/79, A61K 33/38, 1997].

Disadvantages of poviargol: in the presence of high antimicrobial activity, the drug has poorly expressed reparative properties, which can negatively affect the healing time.

The objective of the invention is to provide a means with multifunctional activity, combining high antimicrobial and high reparative activity and accelerating the healing of lesions of integumentary tissues and superficial wounds at all stages of the healing process of the wound, in particular as a consequence of burns of various etiologies, as the most severe skin lesions of thermal, chemical, radiation burn, combined lesions. The tool should have the following distinguishing features when used: a reduced latency period, an acceleration of the completion of the inflammatory period, early periods and a shortening of the period of epithelial formation, a decrease or absence of a period of purulent inflammation and a lack of toxicity and allergenicity.

This problem was solved by obtaining a composition containing the following ingredients: as a biologically active substance, a component related to pectins obtained from marine plants of the “Intestinal” family (Zosteraceae) - polygalactoramnuronan (trade names: zosterin, zosterin-ultra 30, zosterin-ultra 60, isosterite), as a component providing high antimicrobial protection, a silver-containing preparation poviargol; distilled water - up to 100 with the following mass ratios, wt.%:

Zosterin - 1-7;

Poviargolum - 0.1-7;

Distilled water - the rest.

The composition of the claimed composition is confirmed by the standard method.

An example of obtaining this composition: to 94 g of distilled water heated to 70 ° C, add 1 g of zosterol powder and dissolve with stirring for 1.5-2 hours. After cooling to room temperature, 5 g of powder, the substance of the drug poviargol, is added to the resulting solution and stirred for 1 hour until completely dissolved.

The effectiveness of the claimed compositions and prototypes was evaluated in experiments performed on white male rats weighing 200-240 g in the treatment model of radiation injury III-A degree. Radiation burns of I, II, III and III-A degrees affect only the upper layers of the skin and therefore are called superficial, local treatment is conservative. At the heart of ensuring the healing of these burns is the prevention and fight against infection of the wound. The morphological structure of the skin of rats corresponds to the morphological structure of the skin of humans, which allows experimental rats to evaluate the effectiveness of external agents for treating human burns.

The animals were kept in a vivarium under standard conditions: air temperature 20-22 ° C, natural light on bedding from sawdust of deciduous trees. In one cage there were no more than 10 rats at a time. The diet of animals consisted of a standard granular feed, which they received 1 time per day from 10.00 to 13.00. Radiation skin lesions were simulated by irradiating a portion of the depilated skin of the back of rats from a source of soft x-ray radiation, the skin irradiation dose was 58 Gy, and the irradiation field was 1.7 × 1.7 cm ² . As experimental studies have shown, the preparations presented for the study were non-toxic, did not have a local irritant effect and were well tolerated by animals with long-term local use. The results of the experiments showed that irradiation of the skin with soft X-ray at a dose of 58 Gy did not have a significant negative effect on the behavior, appetite, weight and stool of animals. Throughout the entire time of observation of animals in all the studied experimental groups, including the control group, the death of animals was not observed. Changes (from the appearance of redness to the development of radiation ulcers) on the depilated skin of rats after irradiation were noted in all experimental and control groups of animals. At the same time, various drugs had different effects on the repair of radiation damage to the skin, namely: a decrease in the duration of the inflammatory period and a decrease in the formation of a crust (ulcer).

Comparative data on the effectiveness of the treatment of radiation injuries of the skin of animals (rats) with solutions of poviargol, zosterol and their compositions are presented below:

Experienced groups Solution composition Signs of radiation damage to the skin (day) Duration of ulcer healing (24 hours) Duration of the inflammatory period Terms of formation of a crust (ulcers) one 1.0% zosterol 4.8 ± 0.5 12.7 ± 0.2 28.3 ± 2.4 2 5.0% Poviargolum 4.0 ± 0.5 11.8 ± 0.4 49.8 ± 8.0 3 5.0% Poviargolum + 1.0% Zosterol four 0.1% poviargol + 7.0% zosterol Without treatment 5 7.0% poviargol + 1.0% zosterol Without treatment the control 4.7 ± 0.8 14.7 ± 0.8 33.0 ± 2.9

Thus, it can be argued that the claimed solution to the complex drug is new according to the claims. Moreover, the positive effect of the proposed solution is that a multifunctional composition has been created that stimulates the reparative process in the integumentary tissues of mammals, has an anti-inflammatory, radioprotective effect, reduces the degree of burn damage, intended for external use in the form of a cutaneous application.

Literature

1. Pediatrics. 1982, No. 10, p.13. Successes of modern biology. - 1991, v. 3, issue 2, p. 302-316.

2. Zavgorudko T.I. The effect of nitrogen-siliceous thermal waters on the autoimmune reaction in children with chronic tonsillitis treated at the Kultsur resort. Pediatrics, 1982, No. 10, p.13;

3. Zavgorudko V.N. and Zavgorudko T.I. Dynamics of immunological parameters in assessing the effectiveness of treatment at resorts with nitrogen-silicon baths in the Far East. Thes. scientific - prakt. conferences Actual issues of balneology in Siberia and the Far East.

4. Goncharenko E.N., Kudryashov Yu.B. Radiation protection products of natural origin. Successes of modern biology. 1991, vol. 3, issue 2, pp. 302-316.

5. Saxonov P.P., Shashkov B.C., Sergeev P.V. Radiation Pharmacology. M .: Medicine, 1976, p. 214-217.

RF patents:

No. 2092077 Composition of ingredients for the herbamarin syrup

No. 2182448 A method of obtaining a food product with therapeutic and prophylactic properties from caviar of aquatic organisms.

No. 2092077 the composition of the ingredients for the balsam syrup "herbamarin"

No. 2340332 A method for the treatment of mastitis in cows.

Claims (1)

A water-soluble bactericidal composition containing the bactericidal substance povargol, characterized in that it contains a mixture of components in the following ratio of components, wt.%:
Zosterin - 1.1-7.0;
Poviargolum - 2.1-7.0;
Distilled water - the rest.