RU2310462C1 - Method for treating and preventing inflammatory diseases of reproductive organs in cows - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: one should inject into dorsal lumbar muscles from the right and from the left against vertebral column per a half-dosage of medicinal preparation that contains the emulsion out of tissues and organs of Black Sea mussels, natural liquid bee honey, 10%-lidocaine hydrochloride solution, potassium iodide, ACD f-2 and formalin at 0.04-0.06 ml/kg body weight at the following ratio of components, g (weight%): emulsion out of tissues and organs of Black Sea mussels 6.0 (41.0), natural liquid bee honey 3.5 (24.0), 10%-lidocaine hydrochloride solution 2.5 (17.1), potassium iodide 2.0 (13.8), ACD f-2 0.5 (3.4), formalin 0.1 (0.7). The innovation enables to activate protective functions of animal body.

EFFECT: higher accuracy and efficiency.

Description

The present invention relates to veterinary medicine, specifically to methods of pathogenetic therapy with tissue and drug-tissue preparations, activating the activity of protective devices of a sick animal organism, for example, cows with delayed afterbirths, postpartum endometritis, mastitis, etc.

Known technical solution according to the patent of the Russian Federation No. 2064791, 08/10/1996

We have not found any reports in the patent and periodic literature on the preparation and use of a preparation containing an emulsion from tissues and organs of the Black Sea mussels, natural bee honey, and medications with painkillers.

The purpose of the invention is to increase therapeutic efficacy due to the complex effect of various biostimulants of animal and vegetable origin on the maximum number of protective devices of a sick body.

This goal is achieved by the fact that in the dorsal muscles of the lumbar of the cow to the left and right of the spinal column, 1/2 part of the dose is administered at the rate of 0.04-0.06 ml / kg of its body weight, a drug containing substances in the following ratio of ingredients in . (wt.%): emulsion from tissues and organs of the Black Sea mussels - 6.0 (41.0), natural bee honey - 3.5 (24.0), 10% solution of lidocaine hydrochloride - 2.5 ( 17.1), potassium iodide - 2.0 (13.8), ASD f-2 - 0.5 (3.4), formalin - 0.1 (0.7).

The technology of preparation of the drug:

- fresh Black Sea mussels are placed in a closed glass container in a refrigerator with a temperature of minus 2-4 ° C for 5-6 days, then kept in a refrigerator at a temperature of plus 2-4 ° C, for 10-12 days their bodies are removed from open shells, minced twice with a meat grinder, unmilled pieces are ground in a porcelain mortar, all the minced meat is mixed with liquid in a glass bowl, where the mussels were located. The resulting mass in a ratio of 1: 1.5 is mixed with 0.9% sodium chloride solution and left for 3-4 hours at room temperature, then it is subjected to heat treatment in a water bath at a temperature of 58-59 ° C, hot filtered through two layers of sterile gauze, kept under sterile conditions, after 24 hours the filtrate is subjected to heat treatment 3-5 times in a water bath at a temperature of 58-59 ° C for an hour, checked for sterility;

- the remaining ingredients are added to the cooled filtrate in compliance with sterility, checked for sterility;

- the resulting preparation is packaged in 14.6 ml in sterile penicillin vials, they are closed with rubber stoppers and metal run-ins and subjected to hourly heat treatment in a water bath at a temperature of 58-59 ° C 3-4 times with a daily interval, checked for sterility;

- keep the drug in closed vials, in a dark, dry room at a temperature of plus 2-4 ° C for up to six months.

Pharmacodynamics of the components of the drug:

- when in unusual conditions for life at a low temperature in the organs and in high-calorie muscles of mollusks containing many vitamins and minerals, biostimulants with different composition and strength are formed. After the donor is introduced into the body, they activate the activity of its protective devices, hormonal, nervous, and buffer systems, and contribute to the normalization of physiological regulation of functions in a sick animal organism [4,6];

- Bee honey, natural liquid (fresh) - a biogenic stimulator of plant flower nectar processed by bees. After the donor is introduced with the drug into the body, it acts as a biostimulant, is a prolongator of the action of biostimulants of animal origin and is a valuable energy material. As a result of such a multifaceted action, it expands and enhances the action of various biostimulants, increases the perception of them with protective devices, its energetic substances increase their tone and performance, the essential substances of fresh honey irritate the nerve endings and, through them, activate the work of protective, buffering, nervous, hormonal and other systems [3];

- a solution of lidocaine hydrochloride has a strong local anesthetic effect, in addition, it affects the normalization of cardiac activity due to the stabilizing effect on the myocardial cellular systems [8,9];

- potassium iodide is readily soluble in water (1: 0.75), does not contain free iodine, does not irritate tissues at the injection site, does not have a bactericidal effect. After absorption in the body of the animal changes with the formation of various iodine compounds. They are part of the thyroid hormones that regulate metabolism, especially protein, activate the activity of protective devices, act bactericidal, anti-inflammatory, promote resorption and neutralization of unoxidized metabolic products [7];

- ASD f-2 is a product of the distillation of meat and bone meal, while its proteins, fats and carbohydrates are gradually broken down to low molecular weight compounds, similar in structure to their structure and action with cell metabolites, contains inorganic and organic nitrogenous substances, choline esters of carboxylic acids. As a result, f-2 ASD has a high biological activity, has a neurotropic cholinomimetic effect on the central and peripheral nervous systems, stimulates the functioning of the hormonal and digestive systems, improves the absorption of nutrients, stimulates the activity of tissue enzymes, accelerates tissue regeneration in a diseased organ or its area, has a strong antiseptic and antimicrobial effect [4, 5, 7];

- formalin is a 40% aqueous solution of formaldehyde with the addition of up to 1% methyl alcohol and formic acid, which protect formalin from polymerization, in the preparation acts as a preservative [1, volume 2, p. 500; 7].

After the drug is introduced into the body of an animal, formaldehyde oxidizes quite quickly to formic acid, partially turns into hexamethylenetetramine, and in the form of these compounds is excreted from the body by the kidneys, gastric glands, and bronchi, acting antiseptically [7].

After administration of the drug at a dose of 0.04-0.06 ml / kg of animal body weight, animal and vegetable biostimulants, its medicinal substances, first, against the background of anesthesia, activate the activity of local, then general protective devices, buffer systems, hormonal and nervous regulation

During experimental verification of therapeutic efficacy in cows with a body weight of 300-350 kg, the drug was injected into the lumbar muscles to the left and to the right of the spinal column, 7.5-9.0 ml. The clinical signs of acute postpartum endometritis disappeared 5-8 days earlier than in cows to which the drug was not administered, the placenta was separated within 30-40 minutes, and in control cows it was often separated by surgery.

We believe that the drug will be widely used in the prevention and treatment of inflammatory diseases of the reproductive organs in cows and other animal species.

Literature

1. Veterinary Encyclopedic Dictionary. M .: Kolos, 1951, volume 2, p. 436, 500.

2. Golikov A.N., Parshutin G.V. Physiology of farm animals. M .: Kolos, 1980, p.205-233.

3. Yorish N.P. Beekeeping products and their use. M .: Rosselkhozizdat, 1976, p.22-50, 86-102.

4. Degtyarev V.I., Parakin V.K., Luzyanin D.Kh. Biogenic stimulants in animal husbandry. Rostov Book Publishing House, 1960, p. 4-9.

5. Deryabin Z.I. Determination of the biological activity of the drug ASD f-2. Veterinary Medicine, 1980, p. 58-59.

6. The life of animals. M .: Education, 1988, volume 2, p. 86-89.

7. Brains I.E. Pharmacology. M .: Selkhozizdat, 1954, p.145, 219, 323, 326, 328, 474.

8. Mashkovsky M.D. Medicines M., 1987, volume 1, p. 128, 329, 539, 584.

9. Pharmacopoeia article 42-3180-95. A solution of lidocaine hydrochloride 1%, 2% and 10% for injection.

Claims (1)

A method for the prevention and treatment of inflammatory diseases of the reproductive organs in cows, including intramuscular administration of a drug containing ASD f-2, characterized in that 1/2 dose is administered in the dorsal muscles of the lumbar of a cow to the left and right of the spinal column at a rate of 0.04 -0.06 ml / kg body weight, a medicinal product additionally containing an emulsion from tissues and organs of the Black Sea mussels, natural bee honey, 10% solution of lidocaine hydrochloride, potassium iodide and formalin in the following ratio ingredients, g (wt.%): emulsion from tissues and organs of the Black Sea mussels - 6.0 (41.0), natural bee honey - 3.5 (24.0), 10% solution of lidocaine hydrochloride - 2, 5 (17.1), potassium iodide - 2.0 (13.8), ASD f-2 - 0.5 (3.4), formalin - 0.1 (0.7).