RU2312669C2 - Method for treating puerperal diseases in cows - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: into dorsal lumbar muscles being from the left and from the right against vertebral column in area between the last thoracic and the first lumbar vertebrae one should introduce per a half dosage at 0.04-0.06 ml/kg animal's body weight a biogenic stimulant that contains, g (weight%): emulsion out of tissues and organs of fresh water mollusks Anodonta cygnaea 5.0 (37.3), fresh natural bee honey 3.0 (22.4), 10%-lidocaine hydrochloride solution 2.0 (15.0), caffeine-sodium benzoate 1.5 (11.2), potassium iodide 1.5 (11.2), ACD f-2 0.3 (2.2), formalin 0.1 (0.7). The innovation enables to obtain high curative effect.

EFFECT: higher efficiency of therapy.

Description

The invention relates to veterinary medicine, specifically to methods of pathogenetic therapy with tissue and drug preparations, activating the activity of protective devices of a sick animal organism.

A known method of treating diseases of humans and animals with tissue preparations containing biostimulants that are formed in the tissues of an organ during conservation during the struggle of living cells for life (1, 4, 7). It was developed for the first time in 1936 by academician V.P. Filatov.

When preparing a tissue preparation (emulsion), a fresh organ of a clinically and hematologically healthy animal is cleaned of excess tissue, placed in a refrigerator with a temperature of 2-4 °. After 6-8 days, it is crushed 2-3 times with a meat grinder, the crushed parts are ground in a porcelain mortar, mixed with 0.9% sodium chloride solution in a ratio of 1: 2. The mixture is left at room temperature for 2-4 hours, kept in a water bath for 60 hours at 60-80 ° C, boiled for 2-3 minutes, filtered hot through 2 layers of sterile gauze, poured into penicillin vials, they are closed with rubber stoppers and metal run-in, autoclave for 60 minutes at 120 ° C or boil fractionally the first time 75, the second time in a day - 45 minutes.

The drug is stored for up to seven months in a dark place at plus 2-4 ° C, injected subcutaneously or intramuscularly to cattle and horses at a dose of 12.0-14.0, pigs - 5.0-8.0, sheep and goats - 2 , 0-4.0 ml once or twice with an interval of 6-7 days.

We have not found any messages in the patent and periodic literature on the preparation and use of emulsions from tissues and organs of freshwater bivalve mollusk toothless, bee honey and medicines with painkillers.

The purpose of the invention is the creation of a drug with a complex effect of enhancing the protective devices of the body of a sick animal.

This goal is achieved by the fact that in the dorsal lumbar muscles of the animal to the left and right of the spinal column in the area between the last pectoral and first lumbar vertebrae, 1/2 of the dose is administered at the rate of 0.04-0.06 ml / kg of animal body weight biogenic stimulator containing g (wt.%): emulsion from tissues and organs of freshwater mollusk Toothless - 5.0 (37.3), fresh bee honey - 3.0 (22.4), 10% lidocaine solution hydrochloride - 2.0 (15.0), caffeine-benzoate sodium - 1.5 (11.2), potassium iodide - 1.5 (11.2), ASD f-2 - 0.3 (2.2) formalin - 0.1 (0.7).

The technology of preparation of the drug:

- fresh, freshwater bivalve mollusk toothless is placed in an open glass container in a refrigerator with a temperature of 2-4 ° C, on day 16-18 their bodies are removed from the open leaves, minced with a meat grinder, ground parts are ground in a porcelain mortar, minced meat is mixed with a double amount of 0 , 9% sodium chloride solution, the liquid of the mollusk bodies is also poured here. The resulting mixture is left for extraction for 3-5 hours at room temperature, then filtered through 3-4 layers of sterile gauze, the filtrate is subjected to heat treatment in a water bath at 58-59 ° C three to four times a day, checked for sterility;

- caffeine-sodium benzoate is dissolved in a cooled sterile filtrate, the remaining components of the drug are added a 10% solution of lidocaine hydrochloride;

- the resulting mixture of 13.4 ml is packaged in sterile penicillin vials, they are closed with rubber stoppers and metal run-in, 3-5 times with a daily interval are treated for an hour in a water bath at 58-59 ° C, checked for sterility;

- keep the drug in closed vials in a dark place at a temperature of plus 2-4 ° C.

Pharmacodynamics of the components of the drug:

- when in unusual conditions and at a low temperature in the cells of the organs and muscles of the bodies of mollusks containing many vitamins, minerals and many other substances, biostimulants of different strength and direction are formed. When they enter the patient’s body (recipient), they activate the activity of their protective devices, hormonal and nervous systems, and contribute to the restoration of physiological regulation of functions in it (1, 2 4, 6, 7);

- natural fresh bee honey - a biogenic stimulant of plant origin, treated with bees, is to some extent also a prolongator of the action of biostimulants and medicinal substances. When a patient enters the body, it splits, supplies it with energetic material, activates, enhances and expands the activity of biostimulants of tissue preparation and honey, its essential oils irritate local nerve endings and, through them, activate and normalize the work of protective devices (3, 7);

- lidocaine hydrochloride has a pronounced local anesthetic effect, and therefore the pain dominant does not occur in the central nervous system, it also has antiarrhythmic properties due to the stabilizing effect on the myocardial cell membranes and the suppression of the automation of ectopic impulses (8, 9);

- caffeine-sodium benzoate normalizes the functioning of the heart, promotes normalization of blood circulation without increasing blood pressure, increases blood flow to the vascular glomeruli of the kidneys and the formation of urine, from which toxic and toxic metabolic products are removed from the patient’s body, increases the efficiency of the brain, especially the cerebral cortex (7);

- potassium iodide does not contain free iodine, does not irritate the nerve endings at the injection site, does not have a bactericidal effect. After absorption in the body of a sick animal, it changes with the formation of various iodine compounds, which are part of the thyroid hormones that regulate metabolism, especially protein, that activate the activity of protective devices, act bactericidal, anti-inflammatory, and promote resorption and neutralization of unoxidized metabolic products (7);

- ASD f-2 is a product of the distillation of meat and bone meal, while proteins, fats, carbohydrates are gradually broken down to low molecular weight compounds similar in chemical structure to cell metabolites, it contains inorganic and organic nitrogenous substances, choline esters of carboxylic acids. As a result, f-2 ASD has a high biological activity, has a neurotropic cholinomimetic effect on the central and autonomic nervous systems, stimulates the functioning of the digestive system, improves the absorption of nutrients, activates the activity of tissue enzymes, accelerates tissue regeneration, and has a strong antiseptic and antimicrobial effect (4, 5, 7);

- formalin is a 40% aqueous solution of formaldehyde with the addition of methyl alcohol and formic acid, which protect formalin from polymerization; in the preparation, it acts as a preservative.

After it enters the animal’s body, formaldehyde oxidizes quite quickly to formic acid, partially turns into hexa-methylenetetramine, and in the form of these compounds is excreted from the animal’s body by the kidneys, gastric glands, bronchi, acting antiseptically (1, p. 500, 7).

After the drug is introduced into the patient’s (recipient's) organism, biostimulants of animal, plant origin and medicinal substances at the beginning activate the activity of local, then general protective devices and buffer systems, hormonal and nervous regulation, improve the functioning of the intestines and other organs, the body of a sick animal enters the fight with a pathogen. During experimental tests, the following were determined:

- an overdose of the drug up to five times does not affect the clinical condition of white mice weighing 27.0-29.0 g, pain was not observed at the injection site of the drug in mice, their body weight increased by 2-4 g more in 10 days than in mice a control group to which the drug was not administered;

- for the prevention of delay in placenta, the cows were administered 1/2 the dose at the rate of 0.05-0.06 ml / kg body weight of the animal to the left and right of the spinal column one day before the birth, no pain was felt at the injection site of the cow, the delivery took place without complications, the placenta was completely separated after the calf;

- after the injection of the drug, the delayed last came out completely for up to 60 minutes, the animals did not feel pain at the injection site of the drug;

- identical drugs were used in the treatment of postpartum endometritis in control and experimental cows, but experimental cows to the left and right of the vertebral column in the area of the posterior slope of the withers were injected with 1/2 of the dose at the rate of 0.05-0.06 ml / kg body weight, the cure of the disease in them occurred on 10-12 days, in the control - on 15-18 days.

We believe that the proposed drug will find wide application in the prevention and treatment of certain postpartum diseases in cows.

Information sources

1. Veterinary Encyclopedic Dictionary. Moscow, Kolos, 1951, Volume 2, p. 436, 500.

2. Golikov A.P., Parshutin G.V. The physiology of agricultural animals. M., "Kolos", 1980, p.205-233.

3. Yorish N.P. Beekeeping products and their use. M., "Rosselkhozizdat", 1976, S. 22-50, 86-102.

4. Degtyarev V.I., Parakin V.K., Luzyanin D.Kh. Biogenic stimulants in animal husbandry. Rostov book publishing house. 1960, p. 4-9.

5. Deryabin Z.I. Determination of the biological activity of the drug ASD f-2. Veterinary Medicine, 1980, p. 58-59.

6. The life of animals. Edited by Pasternak R.K. M., "Enlightenment", 1988, volume 2, pp. 65-78.

7. Brains I.E. Pharmacology. Moscow, Selkhozizdat, 1954, p.145, 219, 323, 326, 328, 474.

8. Mashkovsky M.D. Medicines M., 1987, volume 1 p. 128, 329, 539, 584.

9. Pharmacopoeia article 42-3180-95. A solution of lidocaine hydrochloride 1%, 2% and 10% for injection.

Claims (1)

A method of treating postpartum diseases of cows, comprising administering a biogenic stimulator in the form of a tissue emulsion, characterized in that 1/2 of the dose is administered in the dorsal lumbar muscles to the left and right of the spinal column in the region between the last pectoral and first lumbar vertebrae at the rate of 0 , 04-0.06 ml / kg of animal body weight a biogenic stimulator containing, g (wt.%): Emulsion from tissues and organs of freshwater mollusk Toothless 5.0 (37.3), fresh bee honey, natural 3.0 (22 , 4), 10% solution of lidocaine hydrochloride 2.0 (15.0), coffee sodium n-benzoate 1.5 (11.2), potassium iodide 1.5 (11.2), ASD f-2 0.3 (2.2), formalin 0.1 (0.7).