RU2311913C2 - Method for activating the activity of protective means in sick animals - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: the present innovation deals with introducing a biogenous stimulant as tissue emulsion into dorsal lumbar muscles of a sick animal from the right and from the left against vertebral column in area between the last thoracic and the first lumbar vertebrae per one half of a dosage at the rate of about 0.04-0.06 ml/kg body weight. The suggested biogenous stimulant contains substances at the following ratio of the components: emulsion out of tissues and organs of fresh-water mollusks of Pelecypoda type - Anodonta cygnaea - 5.5 ml, fresh natural bee honey 4.5 g, ACD f-2 0.3 ml, potassium iodide 1.9 g, caffeine sodium benzoate 1.9 g, formalin 0.1 ml. The method activates local and general protective means of a sick animal being highly efficient at prophylaxis and treatment of animal diseases.

EFFECT: higher efficiency of activation.

Description

The present invention relates to veterinary medicine, specifically to methods of pathogenetic therapy with tissue preparations, activating the activity of protective devices of a sick animal organism.

A known method of treating diseases of humans and animals with tissue preparations containing biostimulants that are formed in tissues during the struggle of living cells for life (1, 4, 7). Developed and put into practice for the first time in 1936 by academician V.P. Filatov.

When preparing tissue preparations (emulsions), a fresh organ of a clinically and hematologically healthy animal is cleaned of excess tissue, placed in a refrigerator with a temperature of plus 2-4 ° C. After 6-8 days, it is crushed 2-3 times with a meat grinder, ground in a porcelain mortar, adding 2.0 ml of 0.9% sodium chloride solution to 1.0 g of minced meat. The mixture is left at room temperature for 2-4 hours, kept in a water bath for 60 hours at 60-80 ° C, boiled for 2-3 minutes, filtered hot through two layers of sterile gauze, poured into penicillin vials, which are closed with rubber stoppers and metal run-in, autoclave for 60 minutes at 120 ° C or boil fractionally for the first time for 75 minutes, for the second time in a day - 45 minutes.

The drug is kept for up to 7 months in a dark place at a temperature of 2-4 ° C, injected into cattle and horses subcutaneously or intramuscularly at a dose of 12.0-14.0, pigs - 5.0-8.0, sheep and goats - 2.0-4.0 ml once or twice with an interval of 6-7 days.

We have not found any reports in the periodic and patent literature on the preparation and use of emulsions from tissues and organs of freshwater bivalve lamellum-gill molluscs.

The purpose of the invention is the activation of the protective devices of an organism of a sick animal with biostimulants of tissues and organs of freshwater platelet-gill mollusk-toothless mollusk, essential and energetic substances of fresh bee honey, ASD f-2 biostimulants, sodium caffeine-benzoate.

This goal is achieved by the fact that in the dorsal lumbar muscles of a sick animal to the left and right of the spinal column in the area between the last pectoral and first lumbar vertebrae, 1/2 of the dose is administered at the rate of 0.04-0.06 ml / kg of its weight body mollusk-honey biogenic stimulant containing substances in the following ratio of components, g (wt.%): emulsion from tissues and organs of freshwater bivalve mollusk-toothless 5.5 ml (38.8), honey bee fresh natural 4.0 ml ( 28.2), ASD f-2 1.6 ml (11.3), potassium iodide 1.5 g (10.5), caffeine nat RI-benzoate 1.5 g (10.5), formalin 0.1 ml (0.7).

The technology of preparation of the drug:

- fresh freshwater bivalve toothless shells in an open glass container are placed in a refrigerator with a temperature of 1-3 ° C, on day 16-18 their bodies are removed from the open leaves, chopped with a meat grinder twice, ground parts are ground in a mortar, minced meat is mixed with a double amount of 0 , 9% sodium chloride solution, leave for 3-4 hours at room temperature, then subjected to an hour-long heat treatment in a water bath at a temperature of 58-59 ° C, the hot mixture is filtered through three layers of sterile gauze, the filtrate is exposed 3-4 times after 24 hours ayut heat treated in a water bath with a temperature of 58-59 ° C, checked for sterility;

- in the chilled sterile filtrate, natural fresh bee honey, ASD f-2, potassium iodide, caffeine sodium benzoate, formalin are dissolved;

- the resulting preparation of 14.2 ml is packaged in sterile penicillin bottles, they are closed with rubber stoppers and metal run-ins, they are subjected to heat treatment in a water bath at a temperature of 58-59 ° C 3-4 times with a daily interval, and they are checked for sterility;

- keep the drug in closed vials in a dark place at a temperature of plus 1-3 ° C until six months.

The mechanism of action of the drug:

- when in unusual conditions and at a low temperature in high-calorie muscles and body organs of toothless shells, in vivo cleans by pumping water through themselves containing vitamins A, B, C, D, trace elements iodine, iron, zinc, hormones, metabolic products substances, different protein biostimulants are formed. When they get into the body of a sick animal, they activate the activity of its protective devices, hormonal and nervous systems, and contribute to the restoration of physiological regulation of functions in it (1, 4, 6, 7);

- natural fresh bee honey - a biogenic stimulant of plant origin, processed by bees, is to some extent a proliferator. When a sick animal enters the body, it breaks down, supplies it with energetic material, activates, enhances and expands the scope of activity of biogenic stimulants, its essential oils, through irritation of local nerve endings, help normalize the functioning of protective devices, central nervous system, reduce the activity of pain dominant (3, 7) ;

- ASD f-2 is a product of the distillation of meat and bone meal, while proteins, fats, carbohydrates are gradually broken down to low molecular weight compounds similar in chemical structure to cell metabolites, it contains inorganic and organic nitrogenous substances, choline esters of carboxylic acids. As a result, the f-2 ASD has a high biological activity, has a neurotropic cholinomimetic effect on the central and autonomic nervous systems, stimulates the functioning of the digestive organs, improves the assimilation of nutrients by the donor organism, activates the activity of tissue enzymes, accelerates tissue regeneration, and has a strong antiseptic and antimicrobial effect ( 4, 5, 7);

- potassium iodide - it is soluble in water (1: 0.75), does not contain free iodine, does not irritate the nerve endings of tissues at the injection site, does not have a bactericidal effect. After absorption in the body, it changes with the formation of various iodine compounds, which are part of the thyroid hormones that activate metabolism and protective devices, act bactericidal, anti-inflammatory, and promote resorption and neutralization of unoxidized products (7);

- sodium caffeine-benzoate - normalizes the functioning of the heart, restores normal blood circulation without increasing blood pressure, increases the filtration of urine in the vascular glomeruli of the kidneys and the removal of toxic and harmful products from the body of a sick animal, leading to a decrease in the content of toxic substances in the blood, normalization of the central nervous system, especially cerebral cortex (7);

- formalin is a 40% aqueous solution of formaldehyde with the addition of up to 1% methyl alcohol and formic acid, which protect formalin from polymerization.

After the donor is introduced into the body, formaldehyde oxidizes quite quickly to formic acid, partially turns into hexamethylene tetramine, and in the form of these compounds is excreted from the body by the kidneys, gastric glands, and bronchi, acting antiseptically. The drug is a preservative (7).

After injecting the drug into the donor’s organism, biostimulants of animal and vegetable origin, the drug first activates the local, then the general protective devices of the sick animal’s organism, activates the hormonal and nervous regulation of functions, motor and other activities of the organs of the body, it’s as if released by biostimulants and drugs from a state of forced rest, due to the action of a pathogenic cause, the body “shakes itself,” as it were, and enters into a struggle with it.

Preliminary test data on the effects of biostimulants and other substances of the drug gave quite encouraging results, therefore, the proposed drug will be widely used in veterinary medicine in the prevention and treatment of animal diseases.

Literature

1. Veterinary Encyclopedic Dictionary. Moscow, Kolos, 1951, Volume 2, p. 436, 500.

2. Golikov A.N., Parshutin G.V. Physiology of farm animals. M., "Kolos", 1980, p.205-233.

3. Yorish N.P. Beekeeping products and their use. M., "Rosselkhozizdat", 1976, S. 22-50, 86-102.

4. Degtyarev V.I., Parakin V.K., Luzyanin D.Kh. Biogenic stimulants in animal husbandry. Rostov Book Publishing House, 1960, p. 4-9.

5. Deryabin Z.I. Determination of the biological activity of the drug ASD f-2. "Veterinary Medicine", 1980, p. 58-59.

6. The life of animals edited by Pasternak RK M., "Enlightenment", 1988, volume 2, pp. 65-78.

7. Brains I.E. Pharmacology. M., Selkhozizdat, 1954, p.145, 219, 323, 326, 328, 474.

Claims (1)

Method revitalization guards sick animal organism, comprising administering to a biogenic stimulants tissue emulsions, characterized in that the dorsal lumbar muscle of the animal patient the left and right sides of the spinal column in the area between the last and first thoracic lumbar vertebrae administered in _^1/2 nd part of the dose at the rate of 0.04-0.06 ml / kg of body weight mollusk-honey biogenic stimulator containing substances in the following ratio of components: emulsion from tissues and freshwater organs toothless bivalve mollusk - 5.5 ml, fresh bee honey - 4.5 g, f-2 ASD - 0.3 ml, potassium iodide - 1.9 g, sodium caffeine-benzoate - 1.9 g, formalin 0 , 1 ml.