RU2339366C1 - Method for non-specific resistance stimulation in animals - Google Patents

Abstract

FIELD: medicine, veterinary medicine.

SUBSTANCE: invention relates to the field of veterinary medicine. The method consists in administration of tissue preparation, obtained from healthy animals' organs after their refrigeration storage at 2-4°C, disintegration, and thermal treatment. The organs are minced; then the tissue mix is thoroughly stirred with fresh city water in proportion 1:2 and filtered to give emulsion, which is processed by thermal treatment in water bath at temperature 58-59°C. After cooling formalin, Dorogov's antiseptic-stimulator fraction 2 (ASD f-2), and bee honey are added to the sterile emulsion. To obtain such preparation, heart muscles, spleen, and thymus are taken in proportion of 15, 10, and 5 weight parts respectively; formalin, ASD f-2, and bee honey are added in such proportion, that their contents in the finished preparation by weight (g) and by per cent (wt) were: tissue emulsion - 10 g, 68.6%; ASD f-2 - 1.5 g, 10.3%; formalin - 0.07 g, 0.5%; natural bee honey - 3.0 g, 20.6%. Before administration to animal the preparation is warmed to 37-39°C, stirred and injected intramuscularly in dose of 0.02-0.07 ml/kg body weight, by 1/2 part of dose on both sides of backbone in withers region, once or twice with 7-9 days interval.

EFFECT: development of effective and innocuous method for non-specific resistance stimulation.

Description

The present invention relates to veterinary medicine, specifically to methods of enhancing the activity of non-specific resistance of an animal organism and organs of the circulatory system with tissue preparations.

According to the patent of the Russian Federation No. 2254856 of June 27, 2005, a method for the prevention and treatment of animal diseases is known, which includes introducing into the lumbar muscles to the left and right of the spinal column at 1/2 of the dose at the rate of 0.02-0.05 ml / kg bovine body weight tissue complex preparation containing 10.0 g (68.7%) of a tissue emulsion from 2.5 g of a pregnant or postpartum uterus, 1.5 g of the thyroid, 1.5 g of the parathyroid, 1.5 g thymus, 2.0 g of the pancreas, 2.5 g of the liver of cattle up to 4-5 years of age, after a 5-8-day treatment in to a lodilnik at a temperature of plus 2-4 ° C, twice minced with a meat grinder, dilution of minced meat with tap water in a ratio of 1: 2, 3-4-hour heat treatment with a daily interval of the filtrate in a water bath at 58-59 ° C, 3.0 g (20.6%) fresh bee honey, 1.5 g (10.3%) ASD f-2, 0.06 g (0.4%) formalin, bottle packaging, heat treatment in a water bath for one hour at 58-59 ° С, sterility test, storage at plus 2-4 ° С; after heating to 37-39 ° C and shaking, the drug is administered into the patient's body once or twice with an interval of 7-8 days.

The disadvantages of the known method and preparation are as follows:

- when placed for the period of canning in the refrigerator at plus 2-4 ° C of the original parenchymal organs, lysis and neutralization of certain substances in a humid environment by others takes place and their effect does not appear after administration of the drug to the patient;

- the process of formation of biostimulants and other proteolysis substances during the preservation of organs in the refrigerator was uneven and insufficiently completed in their deep parts due to insufficient air flow.

The purpose of the invention is to increase the therapeutic efficacy of the drug by increasing the content of biostimulants and other active substances during the preservation of the original organs in the refrigerator with uniform tissue aeration and in the process of preparing the drug from them.

This goal is achieved in that the tissue preparation for stimulating non-specific resistance of the animal organism, containing components in the following ratio in g (wt.%): 10.0 g (68.6%) of tissue emulsion of the heart, spleen, thymus gland of cattle up to five years of age, 3.0 g (20.6%) of fresh bee honey, 1.5 g (10.3%) ASD f-2, 0.07 g (0.5%) of formalin are administered intramuscularly to the patient in the posterior region slopes of the withers to the left and to the right of the vertebral column at 1/2 of the dose at the rate of 0.02-0.07 ml / kg body weight of large horned about cattle with cardiac abnormalities, decreased productivity, growth rate, development and other indications.

Preparation technology: 15 parts by weight of the heart muscles, 10 parts by weight of the spleen, 5 parts by weight of the thymus (goiter) gland of cattle are cut with a sharp knife into small pieces of the same size or cut as deep as possible of the same size into sections and place the organs separately in glass containers with a large supply of air, updated repeatedly. They are placed in a refrigerator with a temperature of plus 2-4 ° C; after 6-8 days, liquids are drained from them, the organs are jointly chopped 2-3 times with a meat grinder, the forcemeat is freed from excess tissue fluid, the forcemeat is diluted with a double amount by weight of 0.9% sodium chloride solution, left at room temperature for 2 -3 hours, stirring the contents after 10-15 minutes; after filtration through 2-3 layers of gauze, the liquid from the container is poured into the mincemeat filtrate obtained in the same way and subjected to a daily interval of three to four hours heat treatment in a water bath at 58-59 ° C, setting the filtrate at these intervals in a thermostat with a temperature of 37-39 ° C, checked for sterility, add 10 g of bee honey, 10 g of natural honey, 1.5 g of ASD f-2, 0.07 g of formalin to 10.0 g of tissue fluid from organs, packaged in vials, they are hermetically closed, subjected to 2-3 times with a daily interval of term eskoy treatment in a water bath at 58-59 ° C, checked for sterility, retain the drug at a temperature of 2-4 ° C, before injecting the drug to the patient in vials warmed to 37-39 ° C and mixed thoroughly.

A brief description of the structure, work, use of the body:

- the heart is a hollow muscular organ of a conical shape that has two atria, two ventricles; the right atrium to the right ventricle pumps venous blood, the left - arterial blood, while the right in the left half of the heart contracts in one rhythm, the time of their reduction is called systole, relaxation - diastole, rest time - pause.

The muscle layer of the heart is the most powerful, especially in the left ventricle, built of cross-striated myofibrils surrounded by sarcolems.

The heart muscle is able to be excited from electrical, chemical, thermal and other stimuli and at this time has refractoriness, i.e. inability to respond with stimulation to artificial or natural stimuli of pacemakers (sinoatreoventricular node, Ashof-Tavar node, etc.), but if an extraordinary excitation occurs in the sinus node at the moment when the refractory period is over, an extraordinary reduction occurs - extrasystole, and the pause lasts as much how much time and the usual pause.

When working in the heart, electrical phenomena (biopotentials) occur due to the difference in electric charges between the excited and unexcited parts of the organ.

The work of the heart is subordinated to the influence of mediators of the vagus and sympathetic nerve, while the first slows and weakens, the second accelerates and strengthens the work of the heart. In addition to the nervous regulation of the activity of the heart, humoral hormones and metabolic products are also regulated (1, vol. 2, p. 36-54, 350-352; vol. 1, p. 523-524);

- spleen - a hematopoietic organ, consists of a skeleton, including a superficial capsule and a network of trabeculae interwoven into the body, consisting of cells of connective tissue mixed with elastic and smooth muscle fibers and pulp, filling the spaces between the trabeculae, built of reticular (hematopoietic) tissue, blood vessels and centers of reproduction of lymphocytes and other blood elements.

Lymphocytes are formed in the spleen, possibly monocytes, damaged and old erythrocytes are destroyed with the release of iron, foreign blood cells, microorganisms are destroyed and neutralized, it is a blood depot and, when reduced, throws plasma and blood cells into the bloodstream (1, t. 2- th, p. 344-345);

- The thymus (goiter) gland is an endocrine gland, especially developed in animals up to 4-5 years old; it consists of a glandular epithelium located in lobules, among which special bodies of concentrically layered cells are laid, with the complete removal of the gland, young animals lag behind in growth, development , weakness, exhaustion develops, their resistance to the harmful factors of the external and internal is reduced among them (1, vol. 1, p. 342-343; 2, p. 230).

When an animal gets into unusual conditions, for example, a meat processing plant, where the smell of blood is everywhere, it experiences stress, accompanied by a significant increase in the content of many substances that regulate the complex functioning of the heart, an increase in the accumulation of hormones, adrenaline, the release of blood from the spleen into blood vessels, and the production of thymus

When pieces of the muscles of the heart, spleen, thymus gland and uniform aeration are preserved in the refrigerator, the vast majority of their hormones and other substances remain unchanged, but the substances of the struggle of different cells and parts of the organs for life are formed, which, when ingested, will have a significant impact on the activity hearts, etc., parts of the circulatory system according to the principle of the first contact with the affected areas;

- natural fresh bee honey contains energetic material, is to some extent a prolongator of the action of biostimulants, hormones, and other substances of tissue emulsion, moreover, honey is a vegetable biostimulator processed by bees (3). After the drug is introduced into the patient’s body, it is first locally, then the whole body supplies with carbohydrates, irritates the nerve endings of tissues and nerve plexuses with essential oils, we normalize the course of processes in the body of a sick animal;

- ASD f-2 is obtained by distillation of meat and bone meal in special apparatuses, in which the protein, fats and carbohydrates are gradually broken down to low molecular weight components that are similar in chemical structure to cell metabolites, contains inorganic and organic nitrogen compounds, choline esters of carboxylic acids. Due to such a variety of chemicals, f-2 ASD has a very high biological activity, has a neurotropic cholinomimetic effect on the central and vegetative jugular systems, stimulates the motor and secretory function of the gastrointestinal tract, improves the assimilation of nutrients by the body, activates the activity of tissue enzymes, improves trophic tissue, increases the level and quality of metabolic processes in various pathological areas, accelerates tissue regeneration in them , Has a strong antiseptic and antimicrobial effect (5);

- formalin is an aqueous solution containing 35-37% formaldehyde, up to 8% methyl alcohol and a small amount of formic acid, which protect formalin from polymerization (1, t, 2nd, p. 500).

After it enters the patient’s body with the drug, formalin oxidizes quite quickly to formic acid, partially turns into hexamethylenetetramine and is excreted in the form of these compounds by the kidneys, gastric glands, and bronchi, acting antiseptically (6).

Thus, a tissue preparation after it enters the patient’s body acts extremely diverse on many of its organs, but above all on the organs of the circulatory system and organs that have pathological processes.

During preliminary experimental checks of the safety and effectiveness of the proposed drug, the following was established:

- the drug is completely safe due to the lack of chemicals, the formalin content is negligible;

- after administration in a therapeutic dose, the body weight of white mice increased after 10 days by 5.0-6.0 more than in mice to which the drug was not administered, the body weight in experimental calves a month after injection of the drug to 18% more than in control calves analogues;

- the general clinical condition in calves after administration of the drug was excellent.

We believe that the proposed drug, which contains mainly natural, rather than chemical substances, will find wide application not only as a therapeutic, but also as a general strengthening and activating body function substance.

Contraindication: traumatic cattle reticulopericarditis.

Literature

1. Veterinary Encyclopedic Dictionary. Moscow, Kolos, 1951, vols. 1 and 2, pages are indicated in the text.

2. Polisov A.N., Parshutin G.V. Physiology of farm animals. M., "Spike", p. 35-64, 230.

3. Yorish N.P. Beekeeping products and their use. Rosselkhozizdat, 1967, p.22-50, 86-102.

4. Degtyarev V.I., Parakin V.K., Luzyanin D.Kh. Biogenic stimulants in animal husbandry. Rostov book publishing house, 1960, from 4-9.

5. Deryabin Z.I. Determination of the biological activity of the drug ASD f-2. Veterinary Medicine, 1980, p. 58-59.

6. Brains I.E. Pharmacology. Moscow, Selkhozizdat, 1954, p. 476.

Claims (1)

A method of stimulating non-specific resistance of animals, comprising administering to the animal a tissue preparation obtained from organs of healthy animals after preserving the organs in the refrigerator at a temperature of 2-4 ° C., grinding and heat treatment, after which the organs are ground, and then the resulting tissue mixture is thoroughly mixed with fresh tap water in a ratio of 1: 2, filtered, the resulting emulsion is subsequently subjected to heat treatments in a water bath at a temperature of 58-59 ° C; after cooling, formalin, ASD f-2 and natural bee honey are added to the sterile emulsion, characterized in that the heart muscles, spleen and thymus gland, which are taken in the amount of 15, 10 and 5 parts by weight, are used as starting material. respectively, and formalin, ASD f-2 and natural bee honey are added to the emulsion so that the finished product had the following ratio of components in quantity, g and wt.%: tissue emulsion - 10 g, 68.6%; ASD f-2 - 1.5 g, 10.3%; formalin - 0.07 g, 0.5%; natural bee honey - 3.0 g, 20.6%; before administration to an animal, the preparation is heated to 37-39 ° C, mixed and injected intramuscularly in 1/2 of the dose at the rate of 0.02-0.07 ml / kg of body weight on both sides of the vertebral column in the withers once or 2 times at intervals 7-9 days